CN107279458A - It is a kind of that there is zinc-rich pea small peptide of enhancing immunity of organisms and preparation method thereof - Google Patents
It is a kind of that there is zinc-rich pea small peptide of enhancing immunity of organisms and preparation method thereof Download PDFInfo
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- CN107279458A CN107279458A CN201710572232.8A CN201710572232A CN107279458A CN 107279458 A CN107279458 A CN 107279458A CN 201710572232 A CN201710572232 A CN 201710572232A CN 107279458 A CN107279458 A CN 107279458A
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- zinc
- small peptide
- rich
- pea small
- organisms
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 75
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical group [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 title claims abstract description 61
- 239000011701 zinc Substances 0.000 title claims abstract description 61
- 229910052725 zinc Inorganic materials 0.000 title claims abstract description 61
- 230000036039 immunity Effects 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 230000002708 enhancing effect Effects 0.000 title claims abstract description 10
- 108010084695 Pea Proteins Proteins 0.000 claims abstract description 10
- 235000019702 pea protein Nutrition 0.000 claims abstract description 10
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- 230000000694 effects Effects 0.000 claims description 17
- WHMDKBIGKVEYHS-IYEMJOQQSA-L Zinc gluconate Chemical compound [Zn+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O WHMDKBIGKVEYHS-IYEMJOQQSA-L 0.000 claims description 10
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- 238000010828 elution Methods 0.000 claims description 8
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- CANRESZKMUPMAE-UHFFFAOYSA-L Zinc lactate Chemical compound [Zn+2].CC(O)C([O-])=O.CC(O)C([O-])=O CANRESZKMUPMAE-UHFFFAOYSA-L 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
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- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims description 3
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
- A23P10/35—Encapsulation of particles, e.g. foodstuff additives with oils, lipids, monoglycerides or diglycerides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/011—Hydrolysed proteins; Derivatives thereof from plants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/0004—Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions
- A61K49/0008—Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure
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- A—HUMAN NECESSITIES
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- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/4841—Filling excipients; Inactive ingredients
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
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- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
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- Nutrition Science (AREA)
- Veterinary Medicine (AREA)
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- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Botany (AREA)
- Endocrinology (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Diabetes (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Pathology (AREA)
- Rheumatology (AREA)
- Toxicology (AREA)
- Urology & Nephrology (AREA)
- Inorganic Chemistry (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Cosmetics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of zinc-rich pea small peptide that there is enhancing immunity of organisms to act on and preparation method thereof, belong to biological technical field.It is characterized in that using pea protein as starting material, purify, be concentrated under reduced pressure by chelating enzymolysis, ultrafiltration, resin column, being spray-dried and obtain zinc-rich pea small peptide.Can be as primary raw material, adding other raw materials and auxiliary material, routinely the preparation that accordingly some are arranged is made in formulation method.The present invention using enzymolysis and rich micro- synchronous method carried out, with rich micro- efficiency high, the organic rate of element is high the characteristics of, zinc-rich pea small peptide can significantly increase immunity of organisms.
Description
Technical field
The present invention relates to biological technical field, and in particular to it is a kind of have enhancing immunity of organisms zinc-rich pea small peptide and
Its preparation method.
Background technology
Immunity is the defense mechanism of human body itself, is that human bioequivalence and any foreign matter for eliminating external intrusion are (viral, thin
Bacterium etc.), processing aging, damage, death, the own cells of denaturation and identification and processing vivo mutations cell and virus are infected thin
The ability of born of the same parents.Immunology Today thinks that immunity is to perform this in human bioequivalence and the physiological reaction for excluding " dissident ", human body
Function is immune system.The body of hypoimmunity is easy to infected or cancer stricken, and a variety of causes prevents immune system from just
Protective effect is often played, in the case, easily causes the infection such as bacterium, virus, fungi, therefore hypoimmunity is most direct
Performance is exactly liable to illness.Because often ill, aggravated the consumption of body, thus typically have a delicate constitution, it is malnutritive, spiritual
Dispirited, fatigue and weak, appetite reduction, sleep-disorder etc. are showed, sick, having an injection to take medicine has become homely food.Every time it is sick all
It is lot more time to recover, and usually recurrent exerbation.If things go on like this body and intelligence development can be caused bad, also easily induced
Major disease.Underlying causes are that hypoimmunity or immunity are unsound.When immune function of human body imbalance, or immune system is not
When perfecting, following point will recurrent exerbation：Flu recurrent exerbation, tonsillitis recurrent exerbation, asthma recurrent exerbation, bronchus
Scorching recurrent exerbation, pneumonia recurrent exerbation, diarrhoea recurrent exerbation, so necessarily can't neglect.Improving immunity is simply
It is usually to note in zinc supplementation, daily diet eat as the abundant food of the zinc such as walnut, yolk, raw oyster, marine product more more.
Pea is sweet, mild-natured, returns spleen, stomach, with gas in benefit, antidiarrheal dysentery, adjust battalion to defend, diuresis, subduing inflammation, solution mammary calculus
Malicious the effect of.To tinea pedis, carbuncle swells, alactation, taste discomfort, hiccup vomiting, trusted subordinate's distending pain, it is thirsty let out the illnesss such as dysentery, have one
Fixed dietary function.Pea is a kind of nourishment food, and protein content is 22%.Contained choline contributes to anti-stop in pea
Arteries and veins is hardened；Contained phytohemagglutinin is similar with the effect of agglutinin contained by lima bean, French beans, the red blood cell of energy aggegation human body,
Promote mitosis；The lymphocyte of tumour patient can be activated, lymphotoxin is produced, has nonspecific to various zooblasts
Injury effect.Therefore, pea be particularly suitable for hypoimmunity crowd eat.
Peptide, is derived from albumen, the multi-functional compounds better than albumen, is current international most popular research topic and great
The function factor of development prospect.Peptide is that the polymer formed, egg are connected by covalent peptide bonds by two or more amino acid
The endless fully hydrolyzed product of white matter is also peptide, and usually by the peptide of 2 peptides~10, (, by 2 Amino acid profiles, n peptides are by n amino for 2 peptides
Acid constitute) or relative molecular mass be less than 2000D peptide be referred to as oligopeptides or small peptide (such as glutathione), by more than 10 amino
The referred to as polypeptide of acid composition.Small peptide can be obtained by protein through modes such as enzymolysis, have advantages below compared with protein：1. it is small
Peptide is the courier for embodying information, with a variety of body metabolisms and physiological regulation function：There are immune promotion, hormone control, antibacterial, resist
Virus, lowering blood pressure and blood fat etc. are acted on；2. small peptide activity is higher, in the case of micro and low concentration, can play higher life
Reason is acted on；3. small peptide molecular weight is small, easy to digest to absorb, and edible safety is high.
Trace element is also known as trace element, although content in human body is simultaneously few, but the existence and health with people cease
Manner of breathing is closed, and the life of people is served vital.Their excess intake, deficiency, imbalance or shortage all can different journeys
Degree ground causes the exception of Human Physiology or occurs disease.Trace element most prominent effect be it is closely related with vitality, only
Size or less amount just can play huge physiological action only as match end.It is worth noting that these trace elements are logical
Directly or indirectly it must be supplied in the case of often by soil, but most people tend not to obtain enough micro members by diet
Element.This every kind of trace element has its special physiological function.Although they are minimum in people's in-vivo content, they are to maintaining people
Some conclusive metabolism in body are very necessary.Once having lacked these required trace elements, human body is just
Disease, or even threat to life occurs.Compare it is clear that about 30% disease is directly microelement deficiencies or uneven institute
Cause.
Zinc is the essential trace elements of the human body, but can not be synthesized in vivo, can only be provided by external food.Zinc is human body
The constituent of many important enzymes, synthesis in protein and nucleic acid, safeguards the integrality of red blood cell and in hematopoiesis
All play an important role, be the key element of enhancing development, the especially development to children's cerebral nervous system is most important.
Zinc can improve immunocompetence, and zinc-deficiency can cause some immunologic functions reduction in body, and the easy repeated infection of such as respiratory tract, skin is easy
Mossy tinea etc..The flowers of life for wanting let us is normally opened, and we should just absorb enough Zn-ef ficiencies.
Zinc-rich pea small peptide can not only further improve the absorptivity of Zn-ef ficiency, and can also make one body two class things of intake
Matter (small peptide and trace element), effect that is respective and cooperateing with performance raising immunity of organisms.
The content of the invention
The first object of the present invention is to provide a kind of zinc-rich pea small peptide that there is enhancing immunity of organisms to act on, this hair
The second bright purpose is to provide the zinc-rich pea small peptide and prepared for strengthening the food, health food and medicine of immunity of organisms
In application.
The purpose of the object of the invention first is realized in：
A kind of zinc-rich pea small peptide that there is enhancing immunity of organisms to act on, it is characterised in that use following processing step system
It is standby：
The preparation of zinc-rich pea small peptide：Zinc chloride (or zinc gluconate, or zinc lactate) is dissolved in into deionized water to be configured to
Concentration is 10~15mg/ml solution, in solution and pea protein ratio 8~12:1 ratio adds pea protein, and stirring is equal
It is even, heating boil 10~15 minutes, be cooled to about 30~50 DEG C, with watery hydrochloric acid adjust pH value 1.0~3.0, add 0.5~
2% pepsin, is incubated 1~3 hour, adjusts pH value 7.0~9.0, adds 0.5~2% trypsase, is incubated 3~5 hours,
Boil 10~15 minutes, centrifuge, precipitation is discarded, supernatant passes through the milipore filter ultrafiltration that relative molecular mass is 3KD, permeate
It is added on DA201-C large pore resin absorption columns, with water elution, discards water lotion, then with 50~90% (V/V) ethanol elutions, receive
Collect ethanol eluate, ethanol is recovered under reduced pressure and continues to be concentrated into relative density for 1.10~1.15 (60 DEG C), (air intake is spray-dried
Temperature is 180~200 DEG C, and leaving air temp is 90~100 DEG C), spray powder is collected, zinc-rich pea small peptide is produced.After testing, zinc-rich
Pea small peptide peptide content is 60%~90%, the accumulation rate ＞ 95% of zinc.
The zinc-rich pea small peptide prepared by the above method, for being prepared into following preparations：
(1) zinc-rich pea small peptide is pressed:Corn oil:Beeswax:Lecithin is 10:35:4:1 mixed material, ground, system
It is standby into soft capsule；
(2) zinc-rich pea small peptide is pressed:Polyethylene glycol 400:Propane diols ratio is 10:38:2 mixed materials, it is ground, prepare
Into soft capsule；
(3) zinc-rich pea small peptide, adds customary adjuvant, is prepared into oral liquid, capsule, tablet, granule.
But the invention is not restricted to above-mentioned formulation.Auxiliary material used in preparation process can be replaced with other conventional auxiliary materials, such as
Starch, dextrin, Icing Sugar, honey, glycerine, sodium carboxymethylcellulose etc.；Zinc-rich pea small peptide can also be shared with other medical substances,
The effect of enhancing immunity of organisms is reached jointly.
The second object of the present invention is to provide the purposes of above-mentioned zinc-rich pea small peptide, and it has the work of enhancing immunity of organisms
With it can be used for preparing food, health food and the medicine for improving immunity.
The beneficial effects of the invention are as follows：Zinc-rich pea small peptide of the present invention, prepares simplicity, with low cost；Pea small peptide with it is micro-
Secondary element collaboration plays the effect for improving immunity of organisms, while having that toxic side effect is small, having no drug resistance, to be difficult residual etc. excellent
Point, reaches the pure natural requirement of pollution-free food, can obtain good Social benefit and economic benefit.
The test of pesticide effectiveness
For the effect of the further raising immunity of checking zinc-rich pea small peptide of the present invention, inventor has carried out pharmacodynamics examination
Checking, test method and result are as follows：
(1) to the influence of mouse swimming time
1. test medicine and animal
Zinc-rich pea small peptide, self-control, small peptide containing pea (with total phthaleinometer) is 82.7%, containing zinc 3.08%.Adopt before use
Finite concentration decoction, gastric infusion are made into aseptic deionized water.
Pea small peptide, self-control, small peptide containing pea (with total phthaleinometer) 81.9%.It is made into before use using aseptic deionized water
Finite concentration decoction, gastric infusion.
Zinc gluconate (food-grade, purity ＞ 98%).Finite concentration decoction is made into using aseptic deionized water before use,
Gastric infusion.
Kunming mouse, SPF grades, female, 18~22g of body weight, purchased from Shanghai Slac Experimental Animal Co., Ltd.,
Animal quality certification number：2015000515110.
2. test method and result
Mouse is taken, 4 groups is randomly divided into, every group 10, is respectively：Zinc-rich pea small peptide group (30mg/kg), pea small peptide group
(30mg/kg), zinc gluconate group (6.5mg/kg), blank control group, continuous gavage give 7d, 2 times/d, and in last dose
Each group mouse tail is born a heavy burden by its body weight 1/10 after 1h, a diameter 50cm is then put into respectively, depth of water 40cm, water temperature (16 ±
1) each group animal swimming time is observed in DEG C container, and to sink under water as observation index.It the results are shown in Table 1.
Influence (X ± s) of the table 1 to mouse swimming time
Note：Compared with blank control group, * P<0.O5, * * P<0.O1.
Above result of the test shows：The swimming time of zinc-rich pea small peptide group mouse has aobvious with blank control group comparing difference
Write sex differernce (P<0.O1)；The swimming time of pea small peptide group and zinc gluconate group mouse is compared with blank control group to be had significantly
Sex differernce (P<0.05).Illustrate that zinc-rich pea small peptide can significantly extend mouse swimming time.
(2) to the influence of mouse immune organ weight
1. test medicine and animal be ibid.
2. test method and result
Mouse is taken, 4 groups is randomly divided into, every group 10, is respectively：Zinc-rich pea small peptide group (30mg/kg), pea small peptide group
(30mg/kg), zinc gluconate group (6.5mg/kg), blank control group, continuous gavage give 10d, 2 times/d, and are given in last
Each group mouse is taken off into cervical vertebra after medicine 1h to put to death, thymus gland is taken and spleen is weighed respectively, draw organ index.It the results are shown in Table 2.
Influence (X ± s) of the table 2 to mouse immune organ weight
Note：Compared with blank control group, * P<0.O5, * * P<0.O1.
Above result of the test shows：The thymus gland and index and spleen index and blank control group of zinc-rich pea small peptide group mouse are poor
It is different to have significant difference (P<0.O1)；The thymus gland and index and spleen index and blank control of pea small peptide group and zinc gluconate group mouse
Group, which compares, significant difference (P<0.05).Illustrate that zinc-rich pea small peptide can significantly improve thymus gland and index and spleen index.
Embodiment
Embodiment is set forth below, the present invention is further illustrated, each embodiment is merely to illustrate the present invention, is not intended to limit this hair
It is bright：
Embodiment 1
The preparation of zinc-rich pea small peptide：Zinc chloride (or zinc gluconate, or zinc lactate) is dissolved in into deionized water to be configured to
Concentration is 12mg/ml solution, in solution and pea protein ratio 10:1 ratio adds pea protein, stirs, and heats
Boil 15 minutes, be cooled to about 45 DEG C, pH value 2.0 is adjusted with watery hydrochloric acid, add 1.5% pepsin, be incubated 2 hours, adjust
PH value 8.0 is saved, 1.0% trypsase is added, 4 hours is incubated, boils 15 minutes, is centrifuged, precipitation is discarded, and supernatant passes through relative
Molecular mass is 3KD milipore filter ultrafiltration, and permeate is added on DA201-C large pore resin absorption columns, with water elution, discards washing
Liquid, then with 75% (V/V) ethanol elution, collect ethanol eluate, ethanol is recovered under reduced pressure and continue to be concentrated into relative density be
1.12 (60 DEG C), spray drying (EAT is 180~200 DEG C, and leaving air temp is 90~100 DEG C), collect spray powder, produce
Zinc-rich pea small peptide.
Embodiment 2
The preparation of zinc-rich pea small peptide：Zinc chloride (or zinc gluconate, or zinc lactate) is dissolved in into deionized water to be configured to
Concentration is 14mg/ml solution, in solution and pea protein ratio 8:1 ratio adds pea protein, stirs, heating is boiled
Boiling 10 minutes, is cooled to about 50 DEG C, and pH value 3.0 is adjusted with watery hydrochloric acid, adds 2% pepsin, is incubated 3 hours, adjusts pH
Value 7.5, adds 2% trypsase, is incubated 5 hours, boils 10 minutes, centrifuges, and precipitation is discarded, and supernatant passes through average molecular matter
The milipore filter ultrafiltration for 3KD is measured, permeate is added on DA201-C large pore resin absorption columns, with water elution, discard water lotion, then
With 60% (V/V) ethanol elution, ethanol eluate is collected, it is 1.15 (60 that ethanol, which is recovered under reduced pressure, and continues to be concentrated into relative density
DEG C), spray drying (EAT is 180~200 DEG C, and leaving air temp is 90~100 DEG C) collects spray powder, produces zinc-rich pea
Beans small peptide.
Embodiment 3
Zinc-rich pea small peptide 100g obtained by the method for Example 1, by zinc-rich pea small peptide:Corn oil:Beeswax:Lecithin
Ratio is 10:35:4:1 mixed material, ground 30 minutes, 100 are pressed with gelatin, purified water, glycerine, iron oxide black:100:40:
1 ratio makes soft capsule hide glue gelatin, 40~50 DEG C of control glue box temperature, 35~45 DEG C of heat insulation tank, sprinkler body temperature 30~40
DEG C, be pressed into soft capsule, per intragranular tolerant heavy 0.45g, 17 DEG C~27 DEG C 3~5h of cold air drying sizings, wash ball, drying, select ball,
Packaging, is prepared into soft capsule finished product.
Embodiment 4
Zinc-rich pea small peptide 100g obtained by the method for Example 1, by zinc-rich pea small peptide:Polyethylene glycol 400:Propane diols
Ratio is 10:38:2 mixed materials, ground 30 minutes, 100 are pressed with gelatin, purified water, glycerine, iron oxide black:100:40: 1
Ratio making soft capsule hide glue gelatin, 40~50 DEG C of control glue box temperature, 35~45 DEG C of heat insulation tank, 30~40 DEG C of sprinkler body temperature,
Soft capsule is pressed into, per the tolerant heavy 0.45g of intragranular, 17 DEG C~27 DEG C 3~5h of cold air drying sizings wash ball, drying, select ball, bag
Dress, is prepared into soft capsule finished product.
Embodiment 5
Zinc-rich pea small peptide 100g obtained by the method for Example 2, plus starch to total amount are 200g, are well mixed, fluidisation
Bed granulation, loads capsule, and packaging is prepared into capsule finished product.
Claims (9)
1. a kind of zinc-rich pea small peptide that there is enhancing immunity of organisms to act on, it is characterised in that use following processing step system
It is standby：
The preparation of zinc-rich pea small peptide：It is 10 that zinc chloride or zinc gluconate or zinc lactate, which are dissolved in deionized water to be configured to concentration,
~15mg/ml solution, in solution and pea protein ratio 8~12:1 ratio adds pea protein, stirs, heating is boiled
Boiling 10~15 minutes, is cooled to about 30~50 DEG C, and pH value 1.0~3.0 is adjusted with watery hydrochloric acid, adds 0.5~2% pepsin,
Insulation 1~3 hour, adjusts pH value 7.0~9.0, adds 0.5~2% trypsase, is incubated 3~5 hours, boils 10~15 points
Clock, centrifugation, precipitation is discarded, and supernatant is by the milipore filter ultrafiltration that relative molecular mass is 3KD, and it is big that permeate is added on DA201-C
On macroporous adsorbent resin post, with water elution, water lotion is discarded, then with the ethanol elution of volume ratio 50~90%, collects ethanol eluate,
It is 1.10~1.15 that ethanol and continuing, which is recovered under reduced pressure, and is concentrated into 60 DEG C to determine relative densities, spray drying EAT is 180~
200 DEG C, leaving air temp is 90~100 DEG C, collects spray powder, produces zinc-rich pea small peptide.
2. according to claim 1 have the zinc-rich pea small peptide for improving immunity of organisms effect, it is characterised in that zinc-rich
Pea small peptide peptide content is 60%~90%, and the accumulation rate of zinc is more than 95%.
3. according to claim 1 have the zinc-rich pea small peptide for improving immunity of organisms effect, it is characterised in that chlorination
Zinc can be replaced with zinc gluconate, zinc lactate.
4. according to claim 1 have the zinc-rich pea small peptide for improving immunity of organisms effect, it is characterised in that described
The concentration of salting liquid is 10~15mg/ml.
5. according to claim 1 have the zinc-rich pea small peptide for improving immunity of organisms effect, it is characterised in that described
Salting liquid and pea protein ratio are 8~10:1.
6. according to claim 1 have the zinc-rich pea small peptide for improving immunity of organisms effect, it is characterised in that described
The retention relative molecular mass of milipore filter is 3000Da.
7. according to claim 1 have the zinc-rich pea small peptide for improving immunity of organisms effect, it is characterised in that described
Spray drying EAT is 180~200 DEG C, and leaving air temp is 90~100 DEG C.
8. according to claim 1 have the zinc-rich pea small peptide for improving immunity of organisms effect, it is characterised in for preparing
Into following preparations：
（1）By zinc-rich pea small peptide:Corn oil:Beeswax:Lecithin is 10:35:4:1 mixed material, it is ground, it is prepared into
Soft capsule；
（2）By zinc-rich pea small peptide:Polyethylene glycol 400:Propane diols ratio is 10:38:2 mixed materials, it is ground, it is prepared into soft
Capsule；
（3）Zinc-rich pea small peptide, adds customary adjuvant, is prepared into oral liquid, capsule, tablet, granule.
9. the zinc-rich pea small peptide described in claim 1 is preparing the application in being used to improve the medicine of immunity of organisms.
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Application publication date: 20171024 |
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