CN107279190A - Lilac daphne and Genkwanin prevent and treat the application of honeybee Mites - Google Patents
Lilac daphne and Genkwanin prevent and treat the application of honeybee Mites Download PDFInfo
- Publication number
- CN107279190A CN107279190A CN201710582271.6A CN201710582271A CN107279190A CN 107279190 A CN107279190 A CN 107279190A CN 201710582271 A CN201710582271 A CN 201710582271A CN 107279190 A CN107279190 A CN 107279190A
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- China
- Prior art keywords
- genkwanin
- lilac daphne
- honeybee
- pulvis
- powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- JPMYFOBNRRGFNO-UHFFFAOYSA-N genkwanin Chemical compound C=1C(OC)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(O)C=C1 JPMYFOBNRRGFNO-UHFFFAOYSA-N 0.000 title claims abstract description 113
- SDTOABMYDICPQU-UHFFFAOYSA-N Genkwanin Natural products C=1C(C)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(O)C=C1 SDTOABMYDICPQU-UHFFFAOYSA-N 0.000 title claims abstract description 112
- DZUKXCCSULKRJA-UHFFFAOYSA-N Isopratol Natural products C=1C(OC)=CC=C(C(C=2)=O)C=1OC=2C1=CC=C(O)C=C1 DZUKXCCSULKRJA-UHFFFAOYSA-N 0.000 title claims abstract description 112
- 235000004338 Syringa vulgaris Nutrition 0.000 title claims abstract description 87
- 241001558516 Varroa destructor Species 0.000 title claims abstract description 21
- 244000297179 Syringa vulgaris Species 0.000 title 1
- 241001104043 Syringa Species 0.000 claims abstract description 86
- 239000000843 powder Substances 0.000 claims abstract description 57
- 241000256844 Apis mellifera Species 0.000 claims abstract description 55
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims abstract description 33
- 238000000227 grinding Methods 0.000 claims abstract description 22
- 239000000203 mixture Substances 0.000 claims abstract description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 30
- 238000002156 mixing Methods 0.000 claims description 19
- 238000002360 preparation method Methods 0.000 claims description 16
- 239000005864 Sulphur Substances 0.000 claims description 15
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 claims description 14
- 238000004821 distillation Methods 0.000 claims description 14
- 238000010348 incorporation Methods 0.000 claims description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 10
- 241000317929 Chalcididae Species 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 241000238876 Acari Species 0.000 abstract description 17
- 230000000694 effects Effects 0.000 abstract description 11
- 241000256837 Apidae Species 0.000 abstract description 5
- 102000002322 Egg Proteins Human genes 0.000 abstract description 5
- 108010000912 Egg Proteins Proteins 0.000 abstract description 5
- 210000004681 ovum Anatomy 0.000 abstract description 5
- 241000196324 Embryophyta Species 0.000 abstract description 3
- 241001245280 Tropilaelaps Species 0.000 abstract description 2
- 239000000419 plant extract Substances 0.000 abstract description 2
- 241000934856 Daphne Species 0.000 description 62
- 239000003814 drug Substances 0.000 description 13
- 238000012360 testing method Methods 0.000 description 10
- 229940079593 drug Drugs 0.000 description 8
- 230000003071 parasitic effect Effects 0.000 description 7
- 230000002265 prevention Effects 0.000 description 7
- 210000000952 spleen Anatomy 0.000 description 7
- QXAITBQSYVNQDR-ZIOPAAQOSA-N amitraz Chemical group C=1C=C(C)C=C(C)C=1/N=C/N(C)\C=N\C1=CC=C(C)C=C1C QXAITBQSYVNQDR-ZIOPAAQOSA-N 0.000 description 5
- 229960002587 amitraz Drugs 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 108010023832 Florigen Proteins 0.000 description 4
- 230000000895 acaricidal effect Effects 0.000 description 4
- 239000000642 acaricide Substances 0.000 description 4
- 230000001261 florigenic effect Effects 0.000 description 4
- 239000011261 inert gas Substances 0.000 description 4
- 238000007873 sieving Methods 0.000 description 4
- 241000256845 Apis dorsata Species 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 239000012141 concentrate Substances 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 206010019233 Headaches Diseases 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000857 drug effect Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 231100000869 headache Toxicity 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 230000003449 preventive effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 125000004203 4-hydroxyphenyl group Chemical group [H]OC1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 241001124076 Aphididae Species 0.000 description 1
- 241000256848 Apis florea Species 0.000 description 1
- 241001076188 Apis laboriosa Species 0.000 description 1
- 244000267222 Brasenia schreberi Species 0.000 description 1
- 235000006506 Brasenia schreberi Nutrition 0.000 description 1
- 241001164374 Calyx Species 0.000 description 1
- 241001310717 Daphne genkwa Species 0.000 description 1
- 241000046691 Daphne odora Species 0.000 description 1
- 241000221079 Euphorbia <genus> Species 0.000 description 1
- 241000628997 Flos Species 0.000 description 1
- 240000000249 Morus alba Species 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- 241000409991 Mythimna separata Species 0.000 description 1
- 241000488583 Panonychus ulmi Species 0.000 description 1
- 241000255969 Pieris brassicae Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 241000489523 Veratrum Species 0.000 description 1
- 241000736816 Xanthorhiza Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- -1 acetyl compound Chemical class 0.000 description 1
- 150000001409 amidines Chemical class 0.000 description 1
- 239000010425 asbestos Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- OTAFHZMPRISVEM-UHFFFAOYSA-N chromone Chemical compound C1=CC=C2C(=O)C=COC2=C1 OTAFHZMPRISVEM-UHFFFAOYSA-N 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002481 ethanol extraction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000000915 fish venom Substances 0.000 description 1
- 235000005679 goldenseal Nutrition 0.000 description 1
- 231100000731 ichthyotoxin Toxicity 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- LGZXYFMMLRYXLK-UHFFFAOYSA-N mercury(2+);sulfide Chemical compound [S-2].[Hg+2] LGZXYFMMLRYXLK-UHFFFAOYSA-N 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000024241 parasitism Effects 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 229910052895 riebeckite Inorganic materials 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000000528 statistical test Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Mites (Tropilaelaps spp.) are the important evil mites of Asia Apidae (Apidae), the invention discloses the application that lilac daphne and Genkwanin prevent and treat honeybee Mites, mite killing composition lilac daphne and Genkwanin are plant tissue and plant extracts, it is more environmentally friendly to bee product, it is pollution-free, to honeybee into honeybee, young honeybee, ovum, queen bee safe；Make that lilac daphne pulvis and Genkwanin powder granularity are thinner using ultramicro grinding, utilization rate of active components is higher, it is more excellent than the effect of sublimed sulfur in the effect of preventing and treating honeybee Mites.
Description
Technical field
The present invention relates to honeybee diseases prevention and control field, and in particular to lilac daphne and Genkwanin prevent and treat the application of honeybee Mites.
Background technology
Mites (Tropilaelaps spp.) are the important evil mites of Asia Apidae (Apidae).Its breed it is fast,
The phoresy phase is short, is a kind of honeybee epizoa bigger than varoa mite (Varroa destructor) harmfulness.Mites are original
Host is apis dorsata (Apis dorsata), but also can alternate hose, the apis mellifera of infection Apidae
(A.Mellifera), black apis dorsata (A.laboriosa) and apis florea (A.Florea).
The most common medicament for being used to prevent and treat honeybee Mites domestic at present has two classes：One class is powder product, mainly there is liter
Magnificent sulphur powder and compound distillation sulphur powder；One class is aqua product, mainly there is Amitraz.
Wherein, using distillation sulphur powder and compound distillation sulphur powder preventing and treating Mites, medicinal powder need to be equably sprinkling upon to honeybee road and frame
Liang Shang, can also be applied directly to cover on sub- spleen, but have problems with：
1) powder granularity is thicker, and utilization ratio of drug is not high；
2) it is used for a long time, preventive effect can decrease, pulvis used in amounts is increased；
3) there is certain toxic action to honeybee, be mainly manifested in that ovum is not hatched, queen bee does not lay eggs.
Mites are prevented and treated using aqua product Amitraz, all honeybee spleens in beehive are proposed need to one by one that carrying out spraying controls mite,
But there is problems with：
1) spray operation is carried out one by one, it is time-consuming；
2) prevention effect is poor relative to powder product；
3) Amitraz is bigger to people's toxicity, and residual can influence bee product quality.European Union has been disabled on honeybee.
Lilac daphne, alias：Medicine water shield, flos genkwa, noisy fish fry, silently headache flower, flower, skin of having a headache, asbestos skin, puffed wheat flower, mud
Qiu Shu, the yellow root of Beijing euphorbia, another name for Sichuan Province mulberry, ichthyotoxin, Latin literary fame：Daphne genkwa Sieb.et Zucc. Thymelaeceaes, Daphne fallen leaves are filled
Wood, high 0.3~1 meter, multi-branched；Bark brown, it is hairless；Sprig cylinder, thin thin, many tool wrinkles, sprout yellow green after drying
Or puce, it is close by faint yellow thread pubescence, old branch puce or aubergine, it is hairless.Leaf is to life, dilute alternate, papery, it is avette or
Ovum shape lanceolar is to ellipticity Long Circle, and tip is suddenly sharp or short tapering, base portion wide wedge shape or blunt round, edge full edge, above it is green
Color, dark brown after drying, below light green, yellowish-brown after drying is close by thin,tough silk shape yellow pubescence when young, then only vein base portion when old
Scattered thin,tough silk shape yellow pubescence, style is short or nothing, column cap head, Chinese red.Flesh texture, white, ellipse is contained in harboring
The bottom of calyx cylinder, 3~May of florescence, 6~July of fruiting period.The main bud using lilac daphne.
Genkwanin, chemical name 4H-Benzopyran-4-one, 5-hydroxy-2- (4-hydroxyphenyl), system
Name 5,4'- dihydroxy -7- methoxy flavones, molecular formula C16H12O5.Molecular weight 284.26.1932 by Chinese medicine scholar once
Wide side is isolated from winter daphne tannin plant lilac daphne first.Genkwanin is faint yellow acicular crystal, 293~296 DEG C of fusing point；Its is complete
200 DEG C of acetyl compound fusing point；Permethylated 156 DEG C of compound fusing point.Genkwanin is soluble in methanol, ethanol and acetone, is insoluble in
Water, chloroform, benzene and petroleum ether.
The effect of lilac daphne and Genkwanin also has been reported that the activity of pesticide-germicide, such as CN100466911C based on medicinal,
Surfactant, emulsifying agent, bleeding agent, synergist etc. are added using lilac daphne active component plant acaricide is made, for preventing and treating
On the industrial crops such as fruit tree, vegetables；CN101336648B, breast is compounded by kuh-seng concentrate, black false hellebore concentrate, lilac daphne concentrate
Oil, is made insecticide, to the mythimna separata on crops fruit tree, vegetables, aphid, heart-eating worm, red spider, cabbage caterpillar, sharp wood louse, tetranychid
Deng with obvious preventive effect function.
Lilac daphne and Genkwanin are used for the preventing and treating of honeybee pest and disease damage, especially there is not been reported for the preventing and treating of Mites.
The content of the invention
It is an object of the invention to：Overcoming the existing defect for preventing and treating the most common medicament presence of honeybee Mites, there is provided one kind
Lilac daphne and Genkwanin prevent and treat the application of honeybee Mites, and compared with prior art, this kind of method can solve poison of the medicament to honeybee
Evil influence, improves the prevention effect of Mites, the quality of bee product is not influenceed.
The present invention is achieved through the following technical solutions：
The present invention provides a kind of application process of lilac daphne in honeybee chalcid fly acarid control.Especially, lilac daphne is prepared into Yuan
Pollen agent is used for honeybee chalcid fly acarid control.
The preparation method of the lilac daphne pulvis comprises the following steps：
1) just crush：Lilac daphne adds pulverizer and carries out preliminary crushing；
2) sieve：The lilac daphne just crushed is crossed into 60 mesh sieves, floccule is removed；
3) ultramicro grinding：The lilac daphne pulvis of powder size D90≤23 micron is obtained by ultramicro grinding.
Wherein, the preparation method of the lilac daphne pulvis also includes distillation sulphur powder blend step.Lilac daphne in the lilac daphne pulvis
Mass content be 10~90%, the mass content 90%~10% of sublimed sulfur.The preparation method of the lilac daphne pulvis include with
Lower step：
1) just crush：Lilac daphne adds pulverizer and carries out preliminary crushing；
2) sieve：The lilac daphne just crushed is crossed into 60 mesh sieves, floccule is removed；
3) distillation sulphur powder mixing：Lilac daphne adds distillation sulphur powder mixing after sieving；
4) ultramicro grinding：The lilac daphne pulvis of powder size D90≤23 micron is obtained by ultramicro grinding.
Lilac daphne is through first crushing, ethanol extraction, filtering and concentrating, extraction, distillation recovery, extraction concentration, column chromatography, condensing crystallizing
Genkwanin can be acquired etc. process.Genkwanin be lilac daphne preventing and treating honeybee Mites application key component, therefore the present invention
Additionally provide a kind of application of Genkwanin in honeybee chalcid fly acarid control.Especially Genkwanin pulvis is in honeybee chalcid fly acarid control
Application.
The preparation method of the Genkwanin pulvis comprises the following steps：
1) Genkwanin premix：Genkwanin, citric acid, talcum powder are sequentially added into mixing apparatus, incorporation time is many
In 60min；
2) Genkwanin powder：Above-mentioned Genkwanin premix, white carbon, talcum powder are sequentially added into mixing apparatus, mixed
Time is no less than 60min, obtains Genkwanin powder；
3) Genkwanin pulvis：Above-mentioned Genkwanin powder is subjected to ultramicro grinding, the Yuan of powder size D90≤23 micron is obtained
Florigen pulvis.
The mass content of Genkwanin is 1~10% in the Genkwanin pulvis.
Sublimed sulfur is also added with the Genkwanin pulvis.Genkwanin pulvis preparation method containing sublimed sulfur includes following
Step：
1) Genkwanin premix：Genkwanin, citric acid, sublimed sulfur are sequentially added into mixing apparatus, incorporation time is many
In 60min；
2) Genkwanin powder：Above-mentioned Genkwanin premix, white carbon, sublimed sulfur are sequentially added into mixing apparatus, mixed
Time is no less than 60min, obtains Genkwanin powder；
3) Genkwanin pulvis：Above-mentioned Genkwanin powder is subjected to ultramicro grinding, the Yuan of powder size D90≤23 micron is obtained
Florigen pulvis.
The Genkwanin powder containing sublimed sulfur, wherein, the mass content of Genkwanin is 1~10%, the matter of sublimed sulfur
Measure content 83%~74%.
The dispensing mode of the lilac daphne pulvis and Genkwanin pulvis is Sa Feng roads and grid beam, can also be applied directly to capping
On spleen.
The present invention compared with prior art, with advantages below and beneficial effect：
1) lilac daphne and Genkwanin are applied to the excellent effect of preventing and treating honeybee Mites；
2) powder size D90≤23 micron of pulvis, its fine size, utilization rate of active components is high；
3) lilac daphne and Genkwanin are used in honeybee chalcid fly acarid control, to honeybee into honeybee, young honeybee, ovum, queen bee safe；
4) mite killing composition lilac daphne and Genkwanin are plant tissue and plant extracts, more environmentally friendly to bee product, pollution-free.
Embodiment
All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive
Speciality and/or step beyond, can combine in any way, unless specifically stated otherwise, can be equivalent by other or with class
Replaced like the alternative features of purpose, i.e. unless specifically stated otherwise, be each characterized as one in a series of equivalent or similar characteristics
Individual embodiment.
Used reagent is mainly as follows in the present invention：Control group acaricide, trade name sublimed sulfur, main component is to rise
Magnificent sulphur (manufacturer is Sichuan Wangshi Bee Health Co., Ltd.).Prevention effect medicament is checked, main component is double first
Amidine (manufacturer is Sichuan Wangshi Bee Health Co., Ltd.).
Embodiment 1：
The preparation of lilac daphne pulvis 1：
1) just crush：Lilac daphne is added into pulverizer and carries out preliminary crushing；
2) sieve：The lilac daphne just crushed is crossed into 60 mesh sieves, floccule is removed；
3) ultramicro grinding：The lilac daphne pulvis 1 of powder size D90≤23 micron is obtained by ultramicro grinding.
Embodiment 2：
The preparation of lilac daphne pulvis 2：
1) just crush：Lilac daphne adds pulverizer and carries out preliminary crushing；
2) sieve：The lilac daphne just crushed is crossed into 60 mesh sieves, floccule is removed；
3) distillation sulphur powder mixing：Lilac daphne (mass content 10%) adds distillation sulphur powder (mass content 90%) and mixed after sieving
Close；
4) ultramicro grinding：Under inert gas shielding, the lilac daphne of powder size D90≤23 micron is obtained by ultramicro grinding
Pulvis 2.
Embodiment 3：
The preparation of lilac daphne pulvis 3：
1) just crush：Lilac daphne adds pulverizer and carries out preliminary crushing；
2) sieve：The lilac daphne just crushed is crossed into 60 mesh sieves, floccule is removed；
3) distillation sulphur powder mixing：Lilac daphne (mass content 90%) adds distillation sulphur powder (mass content 10%) and mixed after sieving
Close；
4) ultramicro grinding：Under inert gas shielding, the lilac daphne of powder size D90≤23 micron is obtained by ultramicro grinding
Pulvis 3.
Embodiment 4：
The preparation of lilac daphne pulvis 4：
1) just crush：Lilac daphne adds pulverizer and carries out preliminary crushing；
2) sieve：The lilac daphne just crushed is crossed into 60 mesh sieves, floccule is removed；
3) distillation sulphur powder mixing：Lilac daphne (mass content 50%) adds distillation sulphur powder (mass content 50%) and mixed after sieving
Close；
4) ultramicro grinding：Under inert gas shielding, the lilac daphne of powder size D90≤23 micron is obtained by ultramicro grinding
Pulvis 4.
Embodiment 5：
The preparation of 1% Genkwanin pulvis：
1) Genkwanin premix：
Mass content ratio：Genkwanin, 20%；Citric acid, 20%；Talcum powder, 60%.
Genkwanin, citric acid, talcum powder are sequentially added into mixing apparatus, incorporation time is no less than 60min；
2) Genkwanin powder：
Mass content ratio：Genkwanin premix, 5%；White carbon, 15%；Talcum powder, 80%.
Above-mentioned Genkwanin premix, white carbon, talcum powder are sequentially added into mixing apparatus, incorporation time is no less than
60min, obtains Genkwanin powder；
3) Genkwanin pulvis：
Above-mentioned Genkwanin powder is subjected to ultramicro grinding, the Genkwanin pulvis of powder size D90≤23 micron is obtained.
Embodiment 6：
The preparation of 2% Genkwanin pulvis：
1) Genkwanin premix：
Mass content ratio：Genkwanin (commercially available, Lanzhou water Lai Si bio tech ltd), 20%；Citric acid,
10%；Talcum powder, 70%.
Genkwanin, citric acid, talcum powder are sequentially added into mixing apparatus, incorporation time is no less than 60min；
2) Genkwanin powder：
Mass content ratio：Genkwanin premix, 10%；White carbon, 15%；Talcum powder, 75%.
Above-mentioned Genkwanin premix, white carbon, talcum powder are sequentially added into mixing apparatus, incorporation time is no less than
60min, obtains Genkwanin powder；
3) Genkwanin pulvis：
Above-mentioned Genkwanin powder is subjected to ultramicro grinding, the Genkwanin pulvis of powder size D90≤23 micron is obtained.
Embodiment 7：
The preparation of 10% Genkwanin pulvis：
1) Genkwanin premix：
Mass content ratio：Genkwanin (is extracted) in lilac daphne, and 20%；Citric acid, 2%；Sublimed sulfur, 78%.
Genkwanin, citric acid, talcum powder are sequentially added into mixing apparatus, incorporation time is no less than 60min；
2) Genkwanin powder：
Mass content ratio：Genkwanin premix, 50%；White carbon, 15%；Sublimed sulfur, 35%.
Above-mentioned Genkwanin premix, white carbon, talcum powder are sequentially added into mixing apparatus, incorporation time is no less than
60min, obtains Genkwanin powder；
3) Genkwanin pulvis：
Above-mentioned Genkwanin powder is subjected to ultramicro grinding under inert gas shielding, powder size D90≤23 micron are obtained
Genkwanin pulvis.
Embodiment 8：
According to《Honeybee acaricide evaluating drug effect field test guideline》, the lilac daphne pulvis and Yuan provided the present invention
Florigen pulvis carries out the prevention effect evaluation of Mites.
Test method：
Bee colony is tested to be provided by Sichuan Wangshi Bee Health Co., Ltd.'s experiment bee farm, selection group gesture consistent (6 spleen),
The consistent bee colony of Mites parasitism level is divided into 8 groups, respectively every group 20 groups, test group 1 (lilac daphne pulvis 1), (Yuan of experimental group 2
Pollen agent 2), experimental group 3 (lilac daphne pulvis 3), experimental group 4 (1% Genkwanin pulvis group), (the 2% Genkwanin pulvis of experimental group 5
Group), experimental group 6 (10% Genkwanin pulvis group), positive controls 7 (sublimed sulfur group) and negative control group 8 (not medication), honeybee kind
For apis mellifera.
Administrated method：Bee colony once, honeybee spleen is sprayed after a cycle medication three times, 21 days every medication in 7 days using Amitraz.
After on-test, daily check collect bottom plate fall mite, before on-test, the 22nd day and the 43rd be preordained primary group (point
Ji Lu ovum, larva, honeybee lid, the quantity of adult honeybee (sufficient frame)), before on-test, the 22nd day and the 43rd day investigation capping
Interior Mites parasitic rate.
Mites parasitic rate in capping：100 capping are chosen out in different sub- spleen regions with tweezers at random out of bee colony,
The bee larva in capping room or pupa are pressed from both sides out, statistics has the capping room number of Mites.
Mites parasitic rate (%)=(the capping room sum for having capping room number/inspection of mite) × 100% in capping
Fall the calculating of mite rate：
Fall mite quantity：Observation once falls mite plate daily after administration, collects and counts mite quantity (a), statistical test terminates
When always fall mite number (∑ a)
Statistics finally falls mite quantity：During off-test, each test group bee colony with excessive Amitraz spray spleen, is united respectively respectively
Meter finally falls mite quantity (b).This quantity (b) plus each bee colony are always fallen into mite number, and (∑ a), as the total mite number of bee colony, is calculated each respectively
Test group bee colony falls mite rate.
Result of the test：
Fall mite rate result see the table below：
Parasitic rate result see the table below：
| Project | Mean parasitized rate % before medication | 22nd day average parasitic rate % after medication | 43rd day average parasitic rate % after medication |
| Lilac daphne pulvis 1 | 5.1 | 1.1 | 0.9 |
| Lilac daphne pulvis 2 | 6.2 | 1.4 | 1.6 |
| Lilac daphne pulvis 3 | 5.8 | 0.9 | 0.8 |
| 1% Genkwanin pulvis | 5.4 | 1.9 | 1.8 |
| 2% Genkwanin pulvis | 4.9 | 0.8 | 0.7 |
| 10% Genkwanin pulvis | 5.7 | 0.5 | 0.4 |
| Sublimed sulfur | 5.3 | 1.5 | 1.4 |
| Negative control | 6.1 | 7.2 | 3.8 |
Group's gesture investigation result see the table below：
Conclusion：
According to《Honeybee acaricide evaluating drug effect field test guideline》, the lilac daphne pulvis and Yuan provided the present invention
Florigen pulvis carry out Mites prevention effect evaluation, as a result for：Lilac daphne pulvis 1, lilac daphne pulvis 2, lilac daphne pulvis 3 and 1%,
2%th, 3% Genkwanin pulvis be averaged mite rate and parasitic rate is superior to from the point of view of sublimed sulfur pulvis, group's gesture situation of bee colony, as a result
Also superior to sublimed sulfur pulvis.Therefore, it is good to the prevention effect of Mites using lilac daphne pulvis and Genkwanin pulvis, and to honeybee
Safety.
It is described above, be only presently preferred embodiments of the present invention, any formal limitation not done to the present invention, it is every according to
According to the present invention technical spirit above example is made any simple modification, equivalent variations, each fall within the present invention protection
Scope.
Claims (10)
1. lilac daphne and Genkwanin prevent and treat the application of honeybee Mites, it is characterised in that：Lilac daphne answering in honeybee chalcid fly acarid control
With.
2. lilac daphne according to claim 1 and Genkwanin prevent and treat the application of honeybee Mites, it is characterised in that the lilac daphne
Being prepared into lilac daphne pulvis is used for honeybee chalcid fly acarid control.
3. lilac daphne according to claim 2 and Genkwanin prevent and treat the application of honeybee Mites, it is characterised in that the lilac daphne
The preparation method of pulvis comprises the following steps：
1) just crush：Lilac daphne adds pulverizer and carries out preliminary crushing；
2) sieve：The lilac daphne just crushed is crossed into 60 mesh sieves；
3) ultramicro grinding：The lilac daphne pulvis of powder size D90≤23 micron is obtained by ultramicro grinding.
4. lilac daphne according to claim 3 and Genkwanin prevent and treat the application of honeybee Mites, it is characterised in that the lilac daphne
The preparation method of pulvis also includes distillation sulphur powder blend step.
5. lilac daphne according to claim 4 and Genkwanin prevent and treat the application of honeybee Mites, it is characterised in that the lilac daphne
The mass content of lilac daphne is 10~90%, the mass content 90%~10% of sublimed sulfur in pulvis.
6. lilac daphne and Genkwanin prevent and treat the application of honeybee Mites, it is characterised in that：The Genkwanin extracted in lilac daphne is small in honeybee
Application in honeybee acarid control.
7. lilac daphne according to claim 6 and Genkwanin prevent and treat the application of honeybee Mites, it is characterised in that Genkwanin powder
Application of the agent in honeybee chalcid fly acarid control.
8. lilac daphne according to claim 7 and Genkwanin prevent and treat the application of honeybee Mites, it is characterised in that the lilac daphne
The preparation method of plain pulvis comprises the following steps：
1) Genkwanin premix：Genkwanin, citric acid, talcum powder are sequentially added into mixing apparatus, incorporation time is no less than
60min；
2) Genkwanin powder：Above-mentioned Genkwanin premix, white carbon, talcum powder are sequentially added into mixing apparatus, incorporation time
No less than 60min, Genkwanin powder is obtained；
3) Genkwanin pulvis：Above-mentioned Genkwanin powder is subjected to ultramicro grinding, the Genkwanin of powder size D90≤23 micron is obtained
Pulvis.
9. lilac daphne according to claim 7 and Genkwanin prevent and treat the application of honeybee Mites, it is characterised in that the lilac daphne
The preparation method of plain pulvis comprises the following steps：
1) Genkwanin premix：Genkwanin, citric acid, sublimed sulfur are sequentially added into mixing apparatus, incorporation time is no less than
60min；
2) Genkwanin powder：Above-mentioned Genkwanin premix, white carbon, sublimed sulfur are sequentially added into mixing apparatus, incorporation time
No less than 60min, Genkwanin powder is obtained；
3) Genkwanin pulvis：Above-mentioned Genkwanin powder is subjected to ultramicro grinding, the Genkwanin of powder size D90≤23 micron is obtained
Pulvis.
10. lilac daphne and Genkwanin according to claim 8 or claim 9 prevents and treats the application of honeybee Mites, it is characterised in that described
The mass content of Genkwanin is 1~10% in Genkwanin pulvis.
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