CN107271321A - Dietary fiber automatic detection device - Google Patents
Dietary fiber automatic detection device Download PDFInfo
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- CN107271321A CN107271321A CN201710573127.6A CN201710573127A CN107271321A CN 107271321 A CN107271321 A CN 107271321A CN 201710573127 A CN201710573127 A CN 201710573127A CN 107271321 A CN107271321 A CN 107271321A
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- Prior art keywords
- condenser pipe
- crucible
- dietary fiber
- liquid
- regulating valve
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- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 37
- 238000001514 detection method Methods 0.000 title claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 35
- 230000001105 regulatory effect Effects 0.000 claims abstract description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 238000005213 imbibition Methods 0.000 claims abstract description 14
- 239000002699 waste material Substances 0.000 claims abstract description 13
- 238000007789 sealing Methods 0.000 claims abstract description 9
- 238000010438 heat treatment Methods 0.000 claims description 12
- 230000001681 protective effect Effects 0.000 claims description 10
- 238000007664 blowing Methods 0.000 claims description 8
- 239000011521 glass Substances 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 230000000694 effects Effects 0.000 claims description 4
- 239000003292 glue Substances 0.000 claims description 2
- 229920002943 EPDM rubber Polymers 0.000 claims 1
- 238000009833 condensation Methods 0.000 abstract description 12
- 230000005494 condensation Effects 0.000 abstract description 12
- 238000013461 design Methods 0.000 abstract description 7
- 238000012546 transfer Methods 0.000 abstract description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 14
- 238000000034 method Methods 0.000 description 12
- 238000000967 suction filtration Methods 0.000 description 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 9
- 238000001556 precipitation Methods 0.000 description 7
- 238000001035 drying Methods 0.000 description 6
- 239000000706 filtrate Substances 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 235000018102 proteins Nutrition 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 230000008901 benefit Effects 0.000 description 5
- 239000000835 fiber Substances 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000007599 discharging Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- KBPLFHHGFOOTCA-UHFFFAOYSA-N 1-Octanol Chemical compound CCCCCCCCO KBPLFHHGFOOTCA-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 229910052571 earthenware Inorganic materials 0.000 description 2
- 238000012869 ethanol precipitation Methods 0.000 description 2
- 239000006260 foam Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 102000004139 alpha-Amylases Human genes 0.000 description 1
- 108090000637 alpha-Amylases Proteins 0.000 description 1
- 229940024171 alpha-amylase Drugs 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000013404 process transfer Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 230000004580 weight loss Effects 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N5/00—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid
- G01N5/04—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid by removing a component, e.g. by evaporation, and weighing the remainder
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a kind of dietary fiber automatic detection device.It includes condenser pipe, sealing ring, middle support, heat block, regulating valve knob, depression bar, heater handle, display screen, switch, socket, waste liquid port, water inlet and delivery port, multiple condenser pipes are mounted side by side in the frame, condensation bottom of the tube is provided with sealing ring, middle support is provided with below condenser pipe, heat block is provided with below middle support, regulating valve knob is disposed with below heat block, heater handle, heater left handle is provided with depression bar, display screen is provided with the right side of condenser pipe, cabinet side rear portion is provided with switch, socket, waste liquid port, water inlet, delivery port, water inlet, delivery port is directly connected with condenser pipe, waste liquid port is connected by suction pumps and imbibition magnetic valve with crucible holder.Reasonable in design of the present invention, employ integrally-built design, it is to avoid issuable error during sample transfer.
Description
Technical field
The present invention relates to dietary fiber detection technique field, and in particular to a kind of dietary fiber automatic detection device.
Background technology
With the increasingly raising of people's living standard, people increasingly pay attention to health diet.With nutrition and correlation
The deep development of science, the nutritive value of dietary fiber is constantly found and paid close attention to, and the 7th class is regarded as by nutrition educational circles supplement
Nutrient, and traditional six class nutrients --- protein, fat, carbohydrate, vitamin, mineral matter and water are arranged side by side.
It is domestic at present only to detect that coarse-fibred instrument (is used for the crude fiber content of grain, feed and other agricultural byproducts
Determine), not specifically designed for the detecting instrument of dietary fiber.The invention is domestic First dietary fiber automatic detection device,
According to the enzymatic gravimetric method in GB/T5009.88-2014, total dietary fiber in food, soluble dietary fiber and insoluble can be determined
Property dietary fiber.The invention simultaneously, which can also take into account, does coarse-fibred detection.
Enzymatic gravimetric method principle in GB/T5009.88-2014 is as follows：Dry sample is taken, with alpha amylase, protease and Portugal
Polyglycoside enzyme enzymolysis, digestion, goes sample liquid ethanol precipitation after isolating protein and starch, enzymolysis, filtering, residue ethanol and acetone
Washing, material is weighed as total dietary fiber residue after drying；Another materials directly is filtered after above-mentioned three kinds of enzymes enzymolysis, residue
With hot wash, weigh after drying, produce insoluble diedairy fiber residue；Filtrate is with 95% ethanol precipitation, the mistake of 4 times of volumes
Weighed after filter, drying, obtain soluble dietary fiber residue.After residue drying obtained as above is weighed, protein and ash are determined respectively
Point.The residue of total dietary fiber, insoluble diedairy fiber and soluble dietary fiber deducts protein, ash content and blank
Calculate total, insoluble and soluble dietary fiber content in sample.
The method of domestic universal detection dietary fiber is to require to carry out manual behaviour according to GB/T5009.88-2014 at present
Make what is detected, i.e., with test tools such as beaker, crucibles, be accomplished manually the operation such as sample enzymolysis, precipitation, suction filtration, washing, during which also
Shifted in needing sample from beaker to crucible, cumbersome, the requirement to operating personnel is higher.Because human factor may be made
Reliability and repeatability into final detection result be not good.And the invention is complete by all one of the detecting step needed for dietary fiber
Into sample avoids human factor to greatest extent without transfer, all steps by intelligence system control, standard operation flow
Influence, makes detection simpler, accurate, reliable.
All it is split type although also there is the device of detection dietary fiber in the world, sample needs in detection process
Transfer, easily causes detection error.The invention employs totally enclosed structure type, collection enzymolysis, heating, vibration suction filtration, washing
And rinse in one, the innovative technology of stirring is controlled with independent heating and air pressure, while 6 samples can be made, using Large Copacity earthenware
Crucible overall process sample is without transfer.Big screen LCD shows and policer operation process that programming system timing constant temperature is controlled,
So as to reach accurately and rapidly purpose, it is ensured that the reliability and accuracy of test data, while unharmful substance is excessive.
Therefore it is also unique in the world.
For the country is without dietary fiber special detection device at present, detection need to be operated manually, complex steps, stability and can
By the present situation, invention dietary fiber automatic detection device such as property is not good.And the relative structure split type with foreign countries, employ entirety
The design of structure, it is to avoid issuable error during sample transfer.
The content of the invention
In view of the shortcomings of the prior art, the present invention seeks to be to provide a kind of dietary fiber automatic detection dress
Put, reasonable in design, employ integrally-built design, it is to avoid issuable error during sample transfer.
To achieve these goals, the present invention is to realize by the following technical solutions：Dietary fiber automatic detection is filled
Put, including condenser pipe, sealing ring, middle support, heat block, regulating valve knob, depression bar, heater handle, display screen, switch, insert
Seat, waste liquid port, water inlet and delivery port, multiple condenser pipes are mounted side by side in the frame, and condensation bottom of the tube is provided with sealing ring, cold
It is provided with to be provided with below heat block, heat block below middle support, middle support below solidifying pipe and is disposed with crucible holder, regulating valve
Knob, heater handle, heater left handle are provided with the right side of depression bar, condenser pipe and are provided with display screen, cabinet side rear portion
Switch, socket, waste liquid port, water inlet, delivery port are provided with, water inlet, delivery port are directly connected with condenser pipe, and waste liquid port passes through
Suction pumps and imbibition magnetic valve are connected with crucible holder.
Preferably, described condenser pipe, which is provided with the top of six, condenser pipe, is provided with protective case and spring, condenser pipe bottom
Portion is provided with protective case and EPT rubber packing circle.
Preferably, described depression bar one end is provided with handle, the depression bar other end is engaged by hook with hook seat, depression bar with
Axle is connected, and axle is connected by regulating block with 304 joints, and 304 joints are engaged with spring, and condenser pipe is provided with spring.
Preferably, described regulating valve knob two ends connect crucible holder and eight logical glass tubes respectively, air blowing magnetic valve, blow
Air pump, imbibition magnetic valve and suction pumps are also connected with eight logical glass tubes, are provided with above regulating valve knob on crucible holder, crucible holder
It is placed with above crucible, crucible and is provided with condenser pipe.When imbibition magnetic valve obtains electric, now imbibition magnetic valve is opened, suction pumps
Condensation liquid in pipe is suctioned out, it is residual in liquid when liquid is drawn onto to a certain degree by the way that regulation regulating valve knob is threaded into maximum
Slag may block screen pack.Air blowing magnetic valve is now opened, the size of air pressure is adjusted by regulating valve knob, blower pump is by sky
Air-blowing enters in pipeline, and residue is dispelled.Such iterative cycles, most liquid and residue are kept completely separate at last.When sample is digested, beat
Blower pump magnetic valve is driven, blower pump blows air into crucible through crucible holder, now by regulating valve knob, control is blown into air
Flow, makes the liquid in crucible is not counter to emit, and sample is vibrated under the influence of air pressure in liquid, plays a part of stirring.
The advantage of pneumatic stirring：Avoid because agitator affixes sample, and cause sample loss, measurement is produced error.In addition grasp
Make simple, instead of artificial oscillating agents, safer, example reaction is more abundant.
The invention has the advantages that：
1st, shifted without sample, all enzymolysis and washing suction filtration are directly completed on instrument, reduce systematic error；
2nd, 1~6 sample is once tested simultaneously, improves testing efficiency；
3rd, novel air pressure vibration agitating mode, replaces artificial oscillating agents, makes reaction more abundant；
4th, whole test process is by intelligence system control, and standard process, reliability is higher.
Brief description of the drawings
Describe the present invention in detail with reference to the accompanying drawings and detailed description；
Fig. 1 is structural representation of the invention；
Fig. 2 is Fig. 1 side view；
Fig. 3 is internal structure schematic diagram of the invention；
Fig. 4 is the structural representation for condensing jacking system of the invention；
Fig. 5 is Fig. 4 A portions mplifying structure schematic diagram；
Fig. 6 is the structural representation (only having drawn two regulating valve knobs in figure to illustrate) of the pneumatic system of the present invention；
Fig. 7 is software module figure of the invention；
Fig. 8 is flow chart of the invention.
Embodiment
To be easy to understand the technical means, the inventive features, the objects and the advantages of the present invention, with reference to
Embodiment, is expanded on further the present invention.
Reference picture 1-8, present embodiment uses following technical scheme：Dietary fiber automatic detection device, including it is cold
Solidifying pipe 1, sealing ring 2, middle support 3, heat block 4, regulating valve knob 5, depression bar 6, heater handle 7, display screen 8, switch 9, insert
Seat 10, waste liquid port 11, water inlet 12 and delivery port 13, multiple condenser pipes 1 are mounted side by side in framework 17, and the bottom of condenser pipe 1 is set
Sealing ring 2 is equipped with, the lower section of condenser pipe 1 is provided with middle support 3, and the middle lower section of support 3 is provided with heat block 4, and the lower section of heat block 4 is successively
Crucible holder 25, regulating valve knob 5, heater handle 7 are provided with, the left side of heater handle 7 is provided with depression bar 6, the right side of condenser pipe 1
Display screen 8 is provided with, cabinet side rear portion is provided with switch 9, socket 10, waste liquid port 11, water inlet 12, delivery port 13, intake
Mouth 12, delivery port 13 are directly connected with condenser pipe 1, and waste liquid port 11 is connected by suction pumps and imbibition magnetic valve with crucible holder.
Described condenser pipe 1 is provided with six, and the top of condenser pipe 1 is provided with protective case and spring, and the bottom of condenser pipe 1 is set
There are protective case and EPT rubber packing circle 2.
Described one end of depression bar 6 is provided with handle 18, and the other end of depression bar 6 is engaged by hook 19 with hook seat 20, depression bar 6 with
Axle 21 is connected, and axle 21 is connected by regulating block 22 with 304 joints 23, and 304 joints 23 are engaged with spring 24, is set on spring 24
There is condenser pipe 1.
The described two ends of regulating valve knob 5 connect crucible holder and eight logical glass tubes 26, air blowing magnetic valve 14, blower pump respectively
16th, imbibition magnetic valve 27 and suction pumps 28 are also connected with eight logical glass tubes 26, and the top of regulating valve knob 5 is provided with crucible holder, earthenware
Crucible 25 is placed with crucible seat, the top of crucible 25 is provided with condenser pipe 1.
Heating system：Heating system is located at the bottom of equipment, including heater, heater handle, crucible.Wherein heater
For semicircle, positioned at the rear of crucible, the movable of heater is controlled by heater handle, heater and crucible are adjusted with this
Distance, it is ensured that optimal heating effect.
The pneumatic system of present embodiment is by six independent regulating valve knobs, six crucible holders, blower pump, air blowings
Pump magnetic valve, suction pumps, suction pumps solenoid valve block into.Six regulating valve knobs are connected to the lower section of crucible holder, for adjusting stream
The size of amount.Blower pump is connected with blower pump magnetic valve, then is connected by main connecting tube and regulating valve knob.Suction pumps and imbibition
Pump magnetic valve is connected, then is connected by main connecting tube and regulating valve knob.Suction pumps and suction pumps magnetic valve are also connected with discharging tube,
Discharging tube is connected with waste liquid port, can discharge waste liquid through discharging tube.When imbibition magnetic valve obtains electric, now imbibition magnetic valve is beaten
Open, suction pumps will condense liquid in pipe and suction out, by the way that regulation regulating valve knob is threaded into maximum, when liquid is drawn onto to a certain degree
When, residue may block screen pack in liquid.Air blowing magnetic valve is now opened, the big of air pressure is adjusted by regulating valve knob
Small, blower pump is blowed air into pipeline, and residue is dispelled.Such iterative cycles, most liquid and residue are kept completely separate at last.
When sample is digested, blower pump magnetic valve is opened, blower pump blows air into crucible through crucible holder, now by regulating valve knob,
Control is blown into the flow of air, makes the liquid in crucible is not counter to emit, and sample is vibrated under the influence of air pressure in liquid, rises
To the effect of stirring.The advantage of pneumatic stirring：Avoid because agitator affixes sample, and cause sample loss, produce measurement
Raw error.In addition it is simple to operate, artificial oscillating agents are instead of, safer, example reaction is more abundant.
The condensation jacking system of present embodiment includes condenser pipe, condensation tube frame, lifting depression bar.Six condenser pipes
It is mounted side by side in the frame.Protective case is installed at top, and installs spring, and buffering and the work compressed are played when being closed with crucible
With.Bottom, which is installed, installs EPT rubber packing circle in lower protective case, lower protective case, it is ensured that gas is not during crucible heating response
Leakage.Condenser pipe top is equipped with condensed water inlet and outlet.The water inlet connection condensed water water supply connector of first condenser pipe, end
The delivery port connection condensed water water out adapter of the condenser of tail, the water inlet pipe phase of the outlet pipe of remaining condenser pipe and adjacent condenser pipe
Even, six condenser pipes are all linked together successively.It is worth noting that the anterior of condenser pipe is all provided with an addition funnel,
Reagent can easily be added.In continuous mode, such as sample residue is bonded in rinse condenser pipe inwall, can also be distilled by funnel
Water is rinsed back crucible, it is to avoid dietary fiber weight loss causes measurement result error.
Condense tube frame and lifting depression bar and slider-rocker mechanism is constituted by internal connecting rod and frame guide rail.Pressure pressure
Bar, condensation tube frame is vertically moved down, and condenser pipe compresses crucible.Depression bar end is provided with latch hook, can hook the hook seat in cabinet, will
Condenser pipe position is fixed.Latch hook is decontroled, lifts depression bar, condenser pipe is moved up, returns to origin-location, and separate with crucible.
Reference picture 7, intelligence control system：Including display screen, driving plate, controller, heater, condensation tube sensor, electricity
Magnet valve, blow pump, suction pumps and control software.By controller, each part works in order under the program pre-set.Each portion
The working condition of part feeds back to operator by display screen, points out operator to be operated.Such as sensor in condenser pipe will
The real time temperature of condenser pipe is shown on a display screen, allows operator to understand current condensation situation at a glance, then
Operational order is sent by display screen, accomplishes human-computer interaction.
The detection means according to the enzymatic gravimetric method in GB/T5009.88-2014, for determine total dietary fiber in food,
Soluble dietary fiber and insoluble diedairy fiber.Whole device includes heating system, pneumatic system, condensation jacking system and intelligence
Can control system.Sample is under the monitoring of intelligence control system, according to the program set, completes enzymolysis, precipitation, suction filtration, washes
The all operationss such as wash.Heating process Discrete control, every section all can be set heating-up temperature and soaking time.Blow pump and suction pumps pass through
Magnetic valve can make crucible and crucible holder produce air pressure vibration, be easy to sample in crucible fully to react, replace through drive circuit control
Artificial oscillating agents, improve efficiency and security.The addition funnel of condenser pipe front portion design can in test easily
Various reagents are added, and the sample residue being bonded in rinse condenser pipe inwall easily can be rinsed into back crucible, it is to avoid sample weight
Amount loss.Condenser pipe real time temperature is shown on display screen by condensation tube sensor, makes operator very clear to condensation effect.
Intelligence control system monitors heater, blows pump and blow the parts such as pump magnetic valve, suction pumps and suction pumps magnetic valve, makes its orderly work
Make, and working condition is fed back into operator by display screen, operator sends operational order by display screen, realize man-machine mutual
It is dynamic.
Reference picture 8, the operating procedure using this Instrument measuring dietary fiber is as follows：
Drying crucible is cleaned 1. taking, is weighed and record data, then weighs 1.000 ± 0.002g of sample, every kind of sample is parallel
Difference≤0.002 ɡ, and clean free of losses is transferred in crucible, is claimed 2g or so diatomite, is placed on title sample paper, it is stand-by.(every kind of sample
Product two and of poor quality≤0.002g, blank two)
2. connecting instrument turnover water swivel, condensed water is opened, plugs in and line and opens master switch.
3. exhaust pipeline moisture content, method：6 valves are first closed, suction pumps button A4 is then opened, valve is outwarded winding one by one and is blotted
Valve is closed after residual water, air valve button A5 is opened simultaneously in the case of 6 valve Close Alls, then closes suction pumps button, is being blown
Open in 6 each crucible holders of valve inspection whether have residual water while gas, it is necessary to ensure that all anhydrous.
4. close lower valve, the crucible equipped with sample is put into crucible holder center, top against protective case endoporus under condenser pipe,
Pressure handle is simultaneously locked.
5. automatic mode is clicked on display screen puts program setting again, can design temperature and soaking time.According to the form below is set, and
Point " preservation ".
Covered 6. opening on instrument, add buffer solution 20ml one by one in condenser pipe top funnel, then added one by one with pipettor
Enter Thermostable aamylase 50ul, then add n-octyl alcohol 2ml one by one.A5 blower pumps, single fine tuning valve are clicked on a display screen
Knob, by sample in each crucible without precipitation, while foam not on emit and be defined, then add into buffer solution 20ml that (each sample delays
Fliud flushing total amount is 40ml).
7. a computer heating control, into the first stage mode, clicks on (R1~R6) heating key for needing to test.Instrument starts to add
Heat is light to draw intermediate heater handle to ensure each heater all contact crucibles.
8. to alarm, screen display " prompting：First stage enzymolysis has terminated, and please determine to enter next stage " points " really
Program enters next step calmly ".Cooled down.
9. to alarm, screen display " prompting：Second stage cooling has terminated, and please determine to enter next stage " now
Open and covered on instrument, protease (50mg/ml) 100ul is added one by one with pipettor in condenser pipe top funnel.Point " it is determined that " journey
Sequence enters next step second and digested, again fine tuning valve knob, by sample without precipitation, foam not on emit and be defined.
10. to alarm, screen display：" prompting phase III (for the second time) enzymolysis has terminated, and please determine to enter lower single order
Section " is now opened to be covered on instrument, is added 3mol/L acetic acid solution 5ml one by one with pipettor in condenser pipe top funnel, is adjusted PH
For 4.5 (adding indicator 250ul), then addition 100ul amyloglucosidases one by one.Point " it is determined that " program enter next stepping
Enter third time to digest.
11. to alarm, screen display " prompting：Fourth stage (third time) enzymolysis has terminated, and please determine to enter lower single order
Section ".
12. total dietary fiber is determined
12.1. added one by one in condenser pipe upper funnel and be pre-heated to 60 DEG C of 95% ethanol solution 180ml, it is single careful
Slowly fine tuning valve knob is stirred evenly, and is then shut off valve, is waited after valve Close All, closing air pump, point " it is determined that " program entrance
Next step " precipitation at room temperature ".
12.2. to alarm.Screen display " prompting：5th stage (precipitation) terminated, and please determine to enter next program "
Add stand-by 2 grams of diatomite in condenser pipe upper funnel, addition during addition slowly, as far as possible diatomite should not be bonded on funnel,
Swept away again with a small amount of 95% ethanol solution and be bonded at diatomite on funnel, opened the single fine tuning valve of blower pump, carefully slowly blow even sample
Then product and diatomite first close valve without precipitation, wait after valve Close All, turn off air pump.Go out glue on the right of instrument
The mouth of pipe, puts receiving bottle, carries out suction filtration operation.
12.3. suction filtration：Suction pumps A4 is first clicked on screen, then single opening lower valve, suction filtration ethanol solution.And respectively
It is single that filtrate is first received into 150ml in conical flask.Then pour into former condenser tube, note from single all return of upper hopper
Meaning：Conical flask must be shaken when backing into makes filtrate without precipitating and wash triangular flask with the ethanol of 10ml 95% and pour into condensation in the lump
Pipe.Whole valves are opened after the completion of single suction filtration simultaneously, most filtrate is taken out.
12.4. wash：78% ethanol is used respectively, and 95% ethanol and acetone 15ml add washing from condenser pipe upper funnel
Residue is each twice.(same to liquid feeding, suction filtration operation)
12.5. dry：Crucible is dried together with residue in 105 DEG C of baking ovens and stayed overnight.
12.6. cooling is weighed：Crucible is placed in drier and cools down 1h, is weighed and record data.(be sample residue,
Crucible, diatomite gross mass)
12.7. the measure of protein and ash content：Sample residue after weighing, respectively by GB/T5009.5-2010 regulation
Nitrogen is determined (gently to scrape all residuals in crucible with spatula, be transferred in digest tube and do protein determination, add in digest tube
Several glass marbles.)；Determine ash content by GB/T5009.4-2016, i.e., be ashed at 525 DEG C 5h (be not required to transfer sample, can be filling
The crucible of residue is ashed in the lump), take out and cooled down in drier after furnace temperature is down to below 100 DEG C, being precisely weighed quality (is
Sample ash content, crucible, diatomite gross mass).
13. insoluble diedairy fiber is determined
13.1. operated by step 1~11.
13.2. washed twice with 70 DEG C of hot distilled water 10ml, merging filtrate (standby to survey soluble dietary fiber).
13.3. operated by step 12.4~12.7.
14. soluble dietary fiber is determined
14.1. the crucible for cleaning drying is taken to weigh and record data.
14.2. 2g or so diatomite is claimed to be transferred in the crucible weighed.
14.3. the crucible of containing diatomite is operated by step 4.
14.4. the merging filtrate of 13.2 operations is moved into condenser pipe from upper funnel is single.
14.5. operated by step 12.1~12.7.
In summary, the dietary fiber detection means of present embodiment employs totally enclosed structure type, collects enzyme
Solution, heating, suction filtration, wash and rinse in one, instead of artificial operation, make detection process simpler, reliable, accurate.Use air pressure
Vibrate the novel method of stirring, it is ensured that sample fully reacts in crucible.6 samples can be made simultaneously, efficiency is added.Using great Rong
Amount crucible overall process sample is not shifted, and reduces error.In operation, it can be controlled with Segmented timed constant temperature, and have large-size screen monitors
Curtain LCDs monitoring display, so as to reach detection accurately and rapidly purpose.
The general principle and principal character and advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the simply explanation described in above-described embodiment and specification is originally
The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (5)
1. dietary fiber automatic detection device, it is characterised in that including condenser pipe (1), sealing ring (2), middle support (3), heating
Block (4), regulating valve knob (5), depression bar (6), heater handle (7), display screen (8), switch (9), socket (10), waste liquid port
(11), water inlet (12) and delivery port (13), multiple condenser pipes (1) are mounted side by side in framework (17), and condenser pipe (1) bottom is set
It is equipped with below sealing ring (2), condenser pipe (1) and is provided with middle support (3), is provided with heat block (4) below middle support (3), heats
It is disposed with the left of crucible holder, regulating valve knob (5), heater handle (7), heater handle (7) and is provided with below block (4)
Display screen (8) is provided with the right side of depression bar (6), condenser pipe (1), cabinet side rear portion is provided with switch (9), socket (10), waste liquid
Mouth (11), water inlet (12), delivery port (13), water inlet (12), delivery port (13) with condenser pipe (1) by being directly connected；It is useless
Liquid mouthful (11) is connected by suction pumps and imbibition magnetic valve with crucible holder.
2. dietary fiber automatic detection device according to claim 1, it is characterised in that described condenser pipe (1) is set
Have and be provided with protective case and spring at the top of six, condenser pipe (1), condenser pipe (1) bottom is provided with protective case and ethylene-propylene-diene monomer
Glue sealing ring.
3. dietary fiber automatic detection device according to claim 1, it is characterised in that described depression bar (6) one end is set
Handle (18) is equipped with, depression bar (6) other end passes through hook (19) and hook seat 20) it is engaged, depression bar (6) is connected with axle (21), axle
(21) it is connected by regulating block (22) with 304 joints (23), 304 joints (23) are engaged with spring (24), spring is set on (24)
It is equipped with condenser pipe (1).
4. dietary fiber automatic detection device according to claim 1, it is characterised in that described regulating valve knob (5)
Two ends connect crucible holder and eight logical glass tubes (26) respectively, air blowing magnetic valve (14), blower pump (16), imbibition magnetic valve (27) and
Suction pumps (28) are also connected with eight logical glass tubes (26), are provided with crucible holder, crucible holder and are placed with above regulating valve knob (5)
Condenser pipe (1) is provided with above crucible (25), crucible (25)；When imbibition magnetic valve obtains electric, now imbibition magnetic valve is opened, and is inhaled
Liquid pump will condense liquid in pipe and suction out, by the way that regulation regulating valve knob is threaded into maximum, when liquid is drawn onto to a certain degree, liquid
Interior residue may block screen pack；Air blowing magnetic valve is now opened, the size of air pressure, blower pump are adjusted by regulating valve knob
Blow air into pipeline, residue is dispelled.Such iterative cycles, most liquid and residue are kept completely separate at last；In sample enzymolysis
When, blower pump magnetic valve is opened, blower pump blows air into crucible through crucible holder, and now by regulating valve knob, control is blown into
The flow of air, makes the liquid in crucible is not counter to emit, and sample is vibrated under the influence of air pressure in liquid, plays stirring
Effect.
5. dietary fiber automatic detection device according to claim 1, it is characterised in that before described condenser pipe (1)
Portion is all provided with an addition funnel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710573127.6A CN107271321B (en) | 2017-07-14 | 2017-07-14 | Dietary fiber automatic checkout device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710573127.6A CN107271321B (en) | 2017-07-14 | 2017-07-14 | Dietary fiber automatic checkout device |
Publications (2)
| Publication Number | Publication Date |
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| CN107271321A true CN107271321A (en) | 2017-10-20 |
| CN107271321B CN107271321B (en) | 2020-07-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710573127.6A Expired - Fee Related CN107271321B (en) | 2017-07-14 | 2017-07-14 | Dietary fiber automatic checkout device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108020483A (en) * | 2018-01-25 | 2018-05-11 | 杭州神农牧业有限公司 | Neutral detergent fiber measurement device |
| CN112586939A (en) * | 2020-11-27 | 2021-04-02 | 江苏萨普莱斯信息科技有限公司 | Heat-preservation rubber and plastic display device for sale and use method thereof |
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| CN202049082U (en) * | 2011-04-14 | 2011-11-23 | 济南海能仪器有限公司 | Crude fiber test instrument |
| CN202418130U (en) * | 2011-12-07 | 2012-09-05 | 常州霍克展示器材制造有限公司 | Rapid connecting structure for rod member and base |
| CN103364527A (en) * | 2012-03-27 | 2013-10-23 | 中国农业大学 | Device for rapidly determining fiber content of feed |
| CN103698543A (en) * | 2013-12-25 | 2014-04-02 | 上海纤检仪器有限公司 | Automatic detection equipment of dietary fiber |
| CN106706464A (en) * | 2015-11-13 | 2017-05-24 | 上海纤检仪器有限公司 | Milk fat analyzer |
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2017
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202049082U (en) * | 2011-04-14 | 2011-11-23 | 济南海能仪器有限公司 | Crude fiber test instrument |
| CN202418130U (en) * | 2011-12-07 | 2012-09-05 | 常州霍克展示器材制造有限公司 | Rapid connecting structure for rod member and base |
| CN103364527A (en) * | 2012-03-27 | 2013-10-23 | 中国农业大学 | Device for rapidly determining fiber content of feed |
| CN103698543A (en) * | 2013-12-25 | 2014-04-02 | 上海纤检仪器有限公司 | Automatic detection equipment of dietary fiber |
| CN106706464A (en) * | 2015-11-13 | 2017-05-24 | 上海纤检仪器有限公司 | Milk fat analyzer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108020483A (en) * | 2018-01-25 | 2018-05-11 | 杭州神农牧业有限公司 | Neutral detergent fiber measurement device |
| CN112586939A (en) * | 2020-11-27 | 2021-04-02 | 江苏萨普莱斯信息科技有限公司 | Heat-preservation rubber and plastic display device for sale and use method thereof |
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| Publication number | Publication date |
|---|---|
| CN107271321B (en) | 2020-07-10 |
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