CN107267615A - G形夹用于改进的等位基因特异性pcr的用途 - Google Patents

G形夹用于改进的等位基因特异性pcr的用途 Download PDF

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Publication number
CN107267615A
CN107267615A CN201710514760.8A CN201710514760A CN107267615A CN 107267615 A CN107267615 A CN 107267615A CN 201710514760 A CN201710514760 A CN 201710514760A CN 107267615 A CN107267615 A CN 107267615A
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China
Prior art keywords
variant
nucleic acid
oligonucleotides
target sequence
nucleotides
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CN201710514760.8A
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English (en)
Chinese (zh)
Inventor
V.博德普迪
N.舍恩布伦纳
A.湛
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication of CN107267615A publication Critical patent/CN107267615A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201710514760.8A 2011-09-23 2012-09-12 G形夹用于改进的等位基因特异性pcr的用途 Pending CN107267615A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161538650P 2011-09-23 2011-09-23
US61/538650 2011-09-23
CN201280045391.8A CN103797132A (zh) 2011-09-23 2012-09-12 G形夹用于改进的等位基因特异性pcr的用途

Related Parent Applications (1)

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CN201280045391.8A Division CN103797132A (zh) 2011-09-23 2012-09-12 G形夹用于改进的等位基因特异性pcr的用途

Publications (1)

Publication Number Publication Date
CN107267615A true CN107267615A (zh) 2017-10-20

Family

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CN201710514760.8A Pending CN107267615A (zh) 2011-09-23 2012-09-12 G形夹用于改进的等位基因特异性pcr的用途
CN201280045391.8A Pending CN103797132A (zh) 2011-09-23 2012-09-12 G形夹用于改进的等位基因特异性pcr的用途

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Country Status (7)

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US (2) US9476091B2 (https=)
EP (1) EP2758546B1 (https=)
JP (1) JP6050820B2 (https=)
CN (2) CN107267615A (https=)
CA (1) CA2847943C (https=)
ES (1) ES2656961T3 (https=)
WO (1) WO2013041194A1 (https=)

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CN107267615A (zh) 2011-09-23 2017-10-20 霍夫曼-拉罗奇有限公司 G形夹用于改进的等位基因特异性pcr的用途
US9382581B2 (en) 2012-12-13 2016-07-05 Roche Molecular Systems, Inc. Primers with modified phosphate and base in allele-specific PCR
EP2971075B1 (en) * 2013-03-13 2018-08-15 Roche Diagnostics GmbH Methods and compositions for detecting mutations in the human pi3kca (pik3ca) gene
JP2019514372A (ja) * 2016-04-20 2019-06-06 ジェービーエス サイエンス インコーポレイテッド Ｃｔｎｎｂ１およびｈｔｅｒｔにおける突然変異を検出するためのキットおよび方法、ならびにｈｃｃ検出および疾患管理におけるそれらの使用
CN112359112A (zh) * 2020-11-11 2021-02-12 凯杰生物工程（深圳）有限公司 一种用于检测calr基因突变的试剂和试剂盒

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WO2003100035A2 (en) * 2002-04-01 2003-12-04 Isis Pharmaceuticals, Inc. Method for rapid detection and identification of viral bioagents
CN1737162A (zh) * 2005-03-16 2006-02-22 南京中医药大学附属医院(江苏省中医院) 表皮生长因子受体(egfr)基因测序检测方法
CN102186996A (zh) * 2008-10-20 2011-09-14 霍夫曼-拉罗奇有限公司 改进的等位基因特异性扩增

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Also Published As

Publication number Publication date
EP2758546B1 (en) 2017-11-22
CA2847943C (en) 2017-08-29
CA2847943A1 (en) 2013-03-28
ES2656961T3 (es) 2018-03-01
US20160369330A1 (en) 2016-12-22
CN103797132A (zh) 2014-05-14
JP6050820B2 (ja) 2016-12-21
JP2014527823A (ja) 2014-10-23
US9476091B2 (en) 2016-10-25
EP2758546A1 (en) 2014-07-30
US20130078630A1 (en) 2013-03-28
WO2013041194A1 (en) 2013-03-28

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Application publication date: 20171020