CN107242552A - With the health-care food composition for alleviating physical fatigue and strengthen immunity function - Google Patents
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- CN107242552A CN107242552A CN201710551022.0A CN201710551022A CN107242552A CN 107242552 A CN107242552 A CN 107242552A CN 201710551022 A CN201710551022 A CN 201710551022A CN 107242552 A CN107242552 A CN 107242552A
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- ZRHANBBTXQZFSP-UHFFFAOYSA-M potassium;4-amino-3,5,6-trichloropyridine-2-carboxylate Chemical compound [K+].NC1=C(Cl)C(Cl)=NC(C([O-])=O)=C1Cl ZRHANBBTXQZFSP-UHFFFAOYSA-M 0.000 description 1
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- 229920001864 tannin Polymers 0.000 description 1
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- 229960004559 theobromine Drugs 0.000 description 1
- 229960000278 theophylline Drugs 0.000 description 1
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- 239000005495 thyroid hormone Substances 0.000 description 1
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- 235000015961 tonic Nutrition 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/15—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a kind of health-care food composition for having and alleviating physical fatigue and strengthen immunity function, its formula includes:The parts by weight of Guarana extract 12;The parts by weight of American ginseng extract 3;The parts by weight of taurine 83;The parts by weight of L isoleucines 5;The parts by weight of L leucines 1.25;The parts by weight of L valines 2.5;The parts by weight of L tryptophans 0.02;The parts by weight of L phenylalanines 0.02;The parts by weight of L threonines 0.02;The parts by weight of L methionine 0.02;The parts by weight of L lysine hydrochlorides 1;The parts by weight of L asparatates 1;The parts by weight of glycine 12;The parts by weight of L alanine 12;80 parts of navy bean extract.Preparation containing foregoing health-care food composition can be beverage, capsule, tablet, chewable tablets, granule, oral liquid, soft capsule, paste, stream juice and semi-fluid juice.Health products of the present invention are low sugar formula, intake of the human body to sugar can be reduced, while can suppress absorption of the people to starchy material in diet, so as to reduce the risk of the diseases such as blood glucose, blood pressure, obesity.
Description
Technical field
The present invention relates to field of health care food, have more particularly, to one kind and alleviate physical fatigue and strengthen immunity function
Health-care food composition.
Background technology
With the development of the society, the development of urbanization process, the diet of people and habits and customs there occurs it is huge
Transformation, it is incident be some headed by fatigue, hypoimmunity, based on three high (hyperglycaemia, hypertension, highs fat of blood) all
City's type disease.The range of age of morbidity crowd constantly expands, and towards rejuvenation trend development;Crowd's quantity increases year by year.I
The fatiguability crowd of state, which is concentrated mainly on, is engaged in scientific research, TI industries occurrences in human life and reporter, performers, marketer, country
Middle and high layer civil servant, the middle and high layer-management person of enterprise, the incidence of disease is up to 50%.The incidence of disease of maturity-onset diabetes is before the more than ten years
1% rises to current 2.5%, and with annual 1 ‰ speed increase, the death rate has risen to the 3rd, only comes tumour, the heart
After vascular diseases, as one of chronic killer for seriously endangering people's health.At present, the intervention of China's diabetes is also rested on
Drug administration, insulin injection stage.Therefore, some fatigue-relievings, strengthen immunity are developed, while diabetic can be given
The health food taken has great social effect.At present, in the market has many similar products, but still has deficiency, has
A little products with the addition of many sugar and additive to improve mouthfeel, be not suitable for being afraid of sugared crowd, and side effect is more;Some products
Function is single, and specific aim is poor, works slow, security is poor.
The content of the invention
It is an object of the invention to provide a kind of health food group for having and alleviating physical fatigue and strengthen immunity function
Compound, it is with the theoretical basic foundation for product design of traditional methods to keep in good health, and it is main for the addition of the natural plants with bioactivity
Raw material, abundant aminoacid ingredient needed by human is added according to modern nutrition theory, also containing Guarana extract, the West
Conopsea extraction, navy bean extract and vitamin ingredients, it is compounded with multiple nutritional components, with alleviation physical fatigue and enhancing
The function of immunity.Wherein Guarana extract and American ginseng extract are Ministry of Public Health's approvals available for the natural of health food
Plant, this formula has reasonable mixture ratio, and active constituent content is high, and physical fatigue and strengthen immunity work(are alleviated with significant
Can, with the good market competitiveness.Meanwhile, the present invention is low sugar formula, can reduce intake of the human body to sugar, and functional component is white
Semen Phaseoli Vulgaris extract can suppress absorption of the people to starchy material in diet, so as to reduce the diseases such as blood glucose, blood pressure, obesity
The risk of disease.
The problem of to solve to mention in background technology, embodiments of the present invention provide a kind of with alleviation physical fatigue
And the health-care food composition of strengthen immunity function, its formula includes:
With the health-care food composition for alleviating physical fatigue and strengthen immunity function, its formula includes:
With the health-care food composition for alleviating physical fatigue and strengthen immunity function, its formula includes:
With the health-care food composition for alleviating physical fatigue and strengthen immunity function, its formula includes:
With in the health-care food composition for alleviating physical fatigue and strengthen immunity function, the formula also includes:
Vitamin B1 0.06-0.09 parts by weight;
Vitamin B6 0.05-0.09 parts by weight.
For example:The parts by weight of vitamin B1 0.08;The parts by weight of vitamin B6 0.08.
The formula is low sugar formula, is compounded with xylitol, maltitol, antierythrite, but is not limited to above-mentioned three
Person's health sugar.
The present invention's has in the health-care food composition for alleviating physical fatigue and strengthen immunity function, the American Ginseng
The extracting method of extract solution is as follows:
S1, American Ginseng pre-treatment:0.5~1.0cm tablet is cut into after American Ginseng is cleaned;
S2, extraction:100kg American Ginsengs are thrown in 3 tons of multi-function extractors, add 10 times of amount (w/r) 50% edible ethanols, plus
Heat backflow 3hr, collects extract solution, and residue adds the practical alcohol reflux 3hr of 10 times of amounts 50%, collects extract solution, residue adds 8
50% edible ethanol is measured again, and backflow 1hr collects extract solution;
S3, sedimentation I:Merge No. three extract solutions, put in settling vat, settle 24 hours, take supernatant liquid filtering;
S4, concentration I:Decompression during above-mentioned filtered fluid is placed in recovery tower below 90 DEG C, is recycled to no alcohol taste;
S5, sedimentation II:Appropriate 95% ethanol is added in above-mentioned filtered fluid, and is stirred 1 hour, 24 are settled in settling vat
By supernatant liquid filtering after hour;
S6 concentrates II:Filter in recovery tower, below 90 DEG C, be recovered under reduced pressure above-mentioned.Being concentrated into relative density is
1.10~1.20 leaching thick pastes.
S7, drying:Above-mentioned thick medicinal extract is put into clean Stainless Steel disk, in vacuum drying chamber, below 90 DEG C, decompression
Vacuum drying;
S8, crushing:Dried object is crushed with pulverizer, 100 mesh sieves is crossed and produces American ginseng extract solid powder.
The present invention's has in the health-care food composition for alleviating physical fatigue and strengthen immunity function, the Guarana
The extraction process of extraction process is:
S1, Guarana seed is weighed in proportion, extracted 3 times with the alcohol refluxs of 6~10 times of 90% concentration of amount, 3 are extracted every time
Hour, implication in proportion is herein:1 part of Guarana seed, 90% Ethanol Treatment is measured with 6-10 times;
S2, filtering, merging filtrate are recovered under reduced pressure after ethanol, are concentrated under reduced pressure into 60 DEG C, are obtained medicinal extract;
S3, medicinal extract is spray-dried, wherein, EAT is 150-180 DEG C, and leaving air temp is 60-90 DEG C, so that
Obtain being spray-dried fine powder, produce Guarana extraction.
Preparation containing previously described health-care food composition can be beverage, capsule, tablet, chewable tablets, granule,
Oral liquid, soft capsule, paste, stream juice and semi-fluid juice.
The method for preparing previously described preparation is the common process of the formulation.
The described compound basis for alleviating physical fatigue and the health food of strengthen immunity function:
1. American Ginseng:For Araliaceae American Ginseng Panax quinquefolium L. dry root.American Ginseng is extracted
The main powerful composition of thing is compound and the trace elements such as American ginseng saponin.Wherein major physiological active material is American Ginseng soap
Glycosides.Initial stage in 18th century, China began to use American Ginseng, so far the existing history of more than 200 years.
American Ginseng has strengthen immunity, builds up health, anti anoxia, alleviates the effect such as physical fatigue, is that the Ministry of Public Health determines
The article available for health food, there are people quite a lot to carry with American Ginseng or its preparation to alleviate body in daily life
Power fatigue.Research report American Ginseng can promote the secretion of some cell factors further to play immunoregulation effect.It is to exempting from
The immunocompromised that epidemic disease suppresses to cause has significant protective effect.American Ginseng has strengthening by means of tonics, blood-nourishing yin-nourishing, the work(of strengthening the spleen and replenishing qi
Effect, and with extensive biological activity, principle active component is compound and the trace elements such as Radix Panacis Quinquefolii polysaccharide, saponin, can
Panimmunity competent cell is acted on, promotes the secretion of some cell factors further to play immunoregulation effect.It is to exempting from
The immunocompromised that epidemic disease inhibitor is caused has significant protective effect, thus to clinical tumor radiotherapy patient, chronic radiation patient
And a variety of causes causes immunocompromised patient to play effectively auxiliary therapeutic action.
2. functional component Guarana is derived from the natural plants of Amazon, containing caffeine, theophylline, theobromine,
Tannin, gleditsia sinensis alkali, a variety of catechols and other micro substances, are considered as by American Indian of Amazon Basin vouchsafe him over the past thousands of years
Youth, beautiful and good health and a long life sources.Surface is studied, Guarana is, as a kind of composition for alleviating physical fatigue, can to use
In fat-reducing, the fillip of nervous system, the raising of memory, promoting circulation of blood, convergence, prevent Adipogenesis and reduce fat, it is establishing-Yang, thin
Born of the same parents' Conservative restoration (DNA as caused by nitrite is destroyed) and antioxidation.Surface Guarana is studied in very low dosage
(1.2mg/L) can also suppress cell membrane lipid peroxidating process, and (2%) does not produce obvious toxicity work yet under high dosage
With this is to keeping vitality and extension life-span to have highly important meaning.During the natural caffeine contained in Guarana is
Cepbalocathartic in pivot stimulant, can improve intracellular cAMP content, there be its effect during low, middle intake:Excited cerebral cortex,
Inspire enthusiasm, improve its notice, self-confidence and operating efficiency and enthusiasm;Strengthen alertness and reduce tired sense, improve
The vigilant and lasting ability to work of maintenance;Strengthen recognition capability, shorten quickly with selecting the reaction time, and instantaneous note can be improved
Recall power
3. functional component taurine is not only involved in maintaining organismic internal environment stable state, and in maincenter, digestion, reproduction and interior point
Secrete etc. system physiological function normally play have in important adjustment effect, body the maintenance of nearly all normal physiological function and
Regulation is required for the participation of taurine, and its effect can be mentioned in the same breath with the effect of hydrone.《Chinese medicine pandect》In mention, taurine
With anticonvulsant action and analgesic activity.Taurine can adjust central neurotransmitter release and activity, can improve body resist
The ability of radical damage, thyroid hormone metabolism, the regulation to cardiac muscle cell's calcium ion can be influenceed, can be improved or stable body
The concentration of the function amino acid of endoskeleton muscular tissue, therefore taurine has wide in terms of anti-sports fatigue, strengthen immunity
Prospect.
4. functional component navy bean extract extracts from navy bean, one extracted using special abstraction technique from navy bean
Activated feedstock is planted, also referred to as " Starch blockers " or " carbohydrate blocking agent ", because it can suppress alpha-amylase to shallow lake
The digestion of powder, so that absorption of the human body to carbohydrate heat is reduced, when combining appropriate diet and exercise program,
It can promote body weight control.Moreover, being shown according to preliminary research, it can adjust blood glucose and maintain normal level.
5. functional component B B-complex (vitamin B1, B6), regulation metabolism in vivo.Vitamin is to maintain healthy institute
Required class organic compound.This kind of material is in vivo neither constitute the raw material of bodily tissue, nor the source of energy,
But a class Auto-regulator, its important function in metabolism.This kind of material due to that can not synthesize in vivo or synthetic quantity is not enough,
So while requirement is seldom, but must be often by food supply.
- vitamin b1 hydrochloride, is the vitamin purified earliest by people, and material generation is participated in as the coenzyme of many enzymes
Thank, be to participate in the most a kind of vitamin of metabolism in vivo, the metabolism with protein, fat, carbohydrate has substantial connection.Dimension
Hold the eubolism of human body, and nervous system normal physiological function.
- pyridoxine hydrochloride, is the closely related nutrients of a kind of synthesis with vivo protein, the metabolism of amino acid
Matter, it can promote absorption of the human body to vitamin B2, B12 and iron, zinc.
The present invention has the advantages that:
Health products of the present invention are tried using caffeine and total saposins, taurine, total amino acid as functional component through security toxicity
Test, strengthen immunity and function of relieving physical fatigue experiment, hygiene and quality stability experiment show, health products safety
It is nontoxic, with significant strengthen immunity and alleviation physical fatigue healthcare function.Further, since the present invention is low sugar formula, together
When can suppress absorption of the people to starchy material in diet, so as to reduce the wind of the diseases such as blood glucose, blood pressure, obesity
Danger.
Embodiment
In order that the technical means, the inventive features, the objects and the advantages of the present invention are easy to understand, tie below
Specific embodiment is closed, the present invention is expanded on further.
The invention provides a kind of health-care food composition for having and alleviating physical fatigue and strengthen immunity function, it is matched somebody with somebody
Side includes:Guarana extract 8-13 parts by weight;American ginseng extract 1.5-3.6 parts by weight;Taurine 80-86 parts by weight;L-
Isoleucine 4-6 parts by weight;L-Leu 0.8-2 parts by weight;Valine 1-3 parts by weight;L-Trp 0.01-0.06 weights
Measure part;L-phenylalanine 0.01-0.05 parts by weight;L- threonine 0.01-0.04 parts by weight;L-Methionine 0.01-0.07 weight
Part;L lysine HCL 0.5-1.9 parts by weight;ASPARTIC ACID 0.3-1.6 parts by weight;Glycine 8-14 parts by weight;L-
Alanine 9-13 parts by weight;Navy bean extract 76-83 parts by weight.
It is as the optimization of C/C composites to above-mentioned formula:With the health food for alleviating physical fatigue and strengthen immunity function
Composition, its formula includes:Guarana extract 8.5-12.8 parts by weight;American ginseng extract 1.6-3.4 parts by weight;Taurine
80.5-85 parts by weight;ILE 4.3-5.9 parts by weight;L-Leu 0.9-1.9 parts by weight;Valine 1.2-2.8 weights
Measure part;L-Trp 0.01-0.05 parts by weight;L-phenylalanine 0.01-0.04 parts by weight;L-threonine 0.01-0.03 weight
Part;L-Methionine 0.01-0.06 parts by weight;L lysine HCL 0.55-1.8 parts by weight;ASPARTIC ACID 0.4-1.6
Parts by weight;Glycine 8.6-13 parts by weight;ALANINE 9.5-12.9 parts by weight;Navy bean extract 75-82.5 parts by weight.
It is as the further optimization to above-mentioned formula, with the health care for alleviating physical fatigue and strengthen immunity function
Food compositions, its formula includes:Guarana extract 8.9-12.5 parts by weight;American ginseng extract 1.9-3.2 parts by weight;
Taurine 81-84 parts by weight;ILE 4.5-5.8 parts by weight;L-Leu 1-1.8 parts by weight;Valine 1.6-2.6
Parts by weight;L-Trp 0.01-0.04 parts by weight;L- phenylalanine 0.01-0.03 parts by weight;L-threonine 0.01-0.025
Parts by weight;L-Methionine 0.01-0.05 parts by weight;L lysine HCL 0.6-1.5 parts by weight;ASPARTIC ACID 0.6-
1.3 parts by weight;Glycine 9-12.5 parts by weight;ALANINE 0-12.5 parts by weight;Navy bean extract 79-81 parts by weight.
Embodiment 1:Beverage preparation
The following is the content of each functional component in functional component optimum formula, 100ml beverage is made into following ratios:
Drink formula in the present embodiment is in addition to above-mentioned raw materials, in addition to B family vitamin, wherein B1 0.8mg/100ml;
B60.8mg/100ml;, and navy bean extract 0.8g/100ml.
By Guarana extract, American ginseng extract, taurine, ILE, L-Leu, Valine, L- colors
Propylhomoserin, L-phenylalanine, L-threonine, L-Methionine, L lysine HCL, ASPARTIC ACID, glycine, ALANINE
And other auxiliary material (acid, essence etc.) mixed dissolutions → UHT135 DEG C of 3~5 seconds sterilization → blending → carbonating → 4~10 DEG C
Filling and capping → labeling, stamp → packaging and storage.
Obtained product is matched according to embodiment 1, the alleviation physical fatigue and strengthen immunity effect embodied is more preferable.
The health food active constituent content and quality with function of physical fatigue alleviation produced according to this technological process
It is all very stable.The security and function test that the health products are carried out through authoritative department Shanghai Disease Prevention and Control Centre, knot
Fruit shows that the health products safety non-toxic alleviates physical fatigue and strengthen immunity effect with significant.Specific content of the test with
As a result it is as follows:
First, safety testing
The toxicity safety test in the I and II stages of the health products shows:The product safety is nontoxic.Shanghai City prevention from suffering from the diseases control
I and II stage toxicity safety test of the center processed to the health products shows:
(1) chmice acute Oral toxicity is tested, and male and female its mouse oral LD50 values are more than 20000mg/kg b.wt, show this
Health products belong to nontoxic level.
(2) mice bone marrow micronucleus:Dosage reaches 10000mg/kg b.wt, does not detect small to male and female
The cause micronucleus effect of mouse Polychromatic erythrocyte.
(3) mouse inbred strain:Dosage reaches 10000mg/kg b.wt, and the mutagenesis to male mice sperm is not detected
Activity.
(4) Salmonella reversion test:Flat board incorporation methods, dosage reach 5mg/ ware, plus and be not added with S9, to standard testing bacterium TA97,
TA98, TA100 and TA102 do not detect obvious mutagenic activity.Therefore, the health products do not have teratogenesis, mutagenesis and carcinogenicity.
(5) 30 days feeding trials, sample with 100,200 and 400mg/kg b.w t dosage (equivalent to the 25 of recommended dose,
During 50 and 100 times of actual intake), feed rat 30 days, rat grows and internal organs, hematology, ferroheme, red thin
The biochemistry pathological index such as born of the same parents, leucocyte does not detect obvious illeffects, has no obvious harmful effect.The sample feeds examination in 30 days
That tests is maximum it was observed that illeffects dosage (NOAEL values) is 4000mg/kg/d
2nd, function of physical fatigue alleviation is tested
According to the Ministry of Public Health《Health food is examined and assessment technique specification》(2003 editions) are to alleviating physical fatigue health food
Function assessment evaluation criterion, Shanghai Disease Prevention and Control Centre shows by zoopery effect of the health products, of the invention
Health food has significant alleviation physical fatigue and strengthen immunity function.
2.1 experimental animal:ICR kind small white mouses, credit number:SCXK (Shanghai) 2007-0005,18-22 grams of body weight, female,
The cleaning grade animal (raising of our station SPF Animal Houses) provided by Shanghai Slac Experimental Animal Co., Ltd..Experimental animal
Raise 20-25 DEG C of room temperature, relative humidity 40-70%, experimental animal credit number:SYXK (Shanghai) 2007-0008, animal feeding
Material, by Suzhou, double lion experimental animal feed technology Services Co., Ltd provide, registration card number:The E that revives raises new word (2002) 006.
2.2 experimental method:
1) dose design:This sample human body recommended dose is daily 120ml/60kg, and this experiment sets basic, normal, high three agent
Amount group, respectively 10,20,60ml/kg, equivalent to human body recommended dose 5,10,30 times, separately set distilled water blank control group.
2) sample treatment:Because sample recommended dose is excessive, more than animal Greatest load, therefore sample preparation is directly weighed
The test liquid 0.25 that is obtained in method, 0.5,1.5ml add distilled water to 20ml, the confession of as basic, normal, high three dosage groups respectively
Test solution.
3) sample approach is given:Gavage, gavage capacity 0.4ml/20g body weight.
4) tested material is given:Separately sampled product 1000,2000,6000mg, plus distilled water to 200m1 be configured to each group by
Test solution (deposits in 4 DEG C of refrigerators, prepare once within every 3 days), and solvent control group gives sub- distilled water, and each group is daily with 20m1/kgb.wt.
The oral gavage of amount, continuous 30d.
5) influence of the tested material to the weight of animals:Kun Ming mice 160 is taken, by only weighing, by body weight stratified random
It is divided into 16 groups, every group 10.Using every four groups as one experimental group, continuous nursing 30 days is according to dosage designed, each group animal claims weekly
Weigh sb..
6) swimming time is determined:The oral gavage of last is given after tested material 30min, puts the body weight sheet lead of root of the tail portion load 5%
Mouse in swimming trunk, the depth of water be more than 30cm, 25 DEG C of water temperature, 1.0 DEG C of scholar, record mouse since swimming start to death time,
It is used as mouse swimming time.
7) Plasma lactate:Take above-mentioned three dosage groups and each control animals each one according to dosage designed after continuous feed 30 days
Group, surveys blood lactase acid and 30 minutes after last oxygen supply, in 30 DEG C of temperature water went swimming 10 minutes, takes out, and surveys blood lactase acid, and hot blast dries
It is dry, it is quiet 20 minutes after, then survey blood lactase acid.
8) determination of urea nitrogen:Take above-mentioned three dosage groups and each control animals according to dosage designed after continuous feed 30 days each
One group, in 30 minutes after last gavage, mouse is put into 30 DEG C of water went swimmings 90 minutes, taken out, hot-air seasoning makes peace and quiet, taken
Mouse blood, centrifugation takes serum to survey urea nitrogen.
9) liver glycogen is determined:Small white mouse 40 is taken, five groups, every group 10 are randomly divided into.According to dosage design continuous nursing 30
My god, in 30 minutes after last gavage, mouse liver is taken out, hepatic glycogen is surveyed.
2.3 result
2.3.1 the influence to body weight is shown in Table 1
Influence of the table 1 to mouse weight
As seen from the above table:Sample has no significant effect to the weight of animals.
2.3.2 the influence to the mice burden swimming time is shown in Table 2
Influence of the sample of table 2 to the mice burden swimming time。
● * P < 0.05 (Analysis of variance) compared with sugared control group
As seen from the above table, sample 60ml/kg dosage groups have significant difference compared with control group.
2.3.3 the influence to blood lactase acid after mouse movement is shown in Table 3
The influence of blood lactase acid TG-AUC after the mouse movement of table 3
● * P < 0.05 (Analysis of variance) compared with sugared control group
As seen from the above table, sample 60ml/kg dosage groups animal blood lactase acid TG-AUC has significantly compared with sugared control group
Sex differernce.
2.3.4 the influence to serum urea nitrogen after mouse movement is shown in Table 4
Influence of the sample of table 4 to serum urea nitrogen after mouse movement
● * P < 0.05 (Analysis of variance) compared with sugared control group
As seen from the above table, sample 60ml/kg dosage has significant difference compared with control group.
2.3.5 the influence to hepatic glycogen after mouse movement is shown in Table 4
Influence of the sample of table 4 to hepatic glycogen after mouse movement
As seen from the above table, sample 60ml/kg dosage groups have significant difference compared with control group.
2.4 summarize:After orally administration mouse various dose sample day fills it up with board energy drink 30 days, with alleviating, muscle power is tired
Labor function.
3rd, strengthen immunity functional experiment is studied
According to the Ministry of Public Health《Health food is examined and assessment technique specification》(2003 editions) are to alleviating physical fatigue health food
Function assessment evaluation criterion, Shanghai Disease Prevention and Control Centre shows by zoopery effect of the health products, of the invention
Health food has significant strengthen immunity function.
3.1 experimental animal:Cleaning grade ICR kind small white mouses, credit number:SCXK (Shanghai) 2007-0005,17-23 grams of body weight,
Female, the cleaning grade animal provided by Shanghai Slac Experimental Animal Co., Ltd. (raising of this center SPF Animal Houses).It is real
Test 20-25 DEG C of animal feeding room temperature, relative humidity 40-70%, experimental animal credit number:SYXK (Shanghai) 2007-0008, is moved
Thing breeding feed, by Suzhou, double lion experimental animal feed technology Services Co., Ltd provide, registration card number:The E that revives raises new word (2002)
006。
3.2 experimental method:
Dose design:This sample human body recommended dose is daily 120ml/60kg, and this experiment sets basic, normal, high three dosage
Group, be distributed as 10,20,60ml/kg, be respectively equivalent to human body recommended dose 5,10,30 times, control group is with distilled water gavage.
Give sample approach:Gavage, gavage capacity 0.4ml/20g body weight.
3.3 experimental result
3.3.1 influence of three tested materials to mouse weight:It is layered at random by body weight, is divided into 16 groups, every group 10, every four groups
For an experimental group, continuous nursing 30 days is according to dosage designed, phase and experiment end are weighed once respectively in an experiment.
Influence of the sample (immune one group) to the weight of animals (gram)
As seen from the above table, each dosage group of sample is compared with control group, difference that there are no significant.
Influence of the sample (immune two groups) to the weight of animals (gram)
As seen from the above table, each dosage group of sample is compared with control group, difference that there are no significant.
Influence of the sample (immune three groups) to the weight of animals (gram)
As seen from the above table, each dosage group of sample is compared with control group, difference that there are no significant.
Influence of the sample (immune four groups) to the weight of animals (gram)
As seen from the above table, each dosage group of sample is compared with control group, difference that there are no significant.
3.1.1. serolysin test:
Animal is continuously to sample after 30 days, after animal being immunized 5 days with 2% (V/V) SRBC, and eye socket blood sampling, cervical dislocation is put to death,
Separate serum.On 96 hole Microhemagglutination plates plus 25 μ l physiological saline, then in first row add 25 μ l serum respectively, each row makees later
Two-fold dilution, adds the μ L of 1%SRBC 100 per hole, is placed 3 hours for 37 DEG C after vibration, observation knot after blood cell control sinks
Really.
Serolysin test
● P < 0.05 (Analysis of variance) compared with control group
Through statistical analysis:Sample 60ml/kg dosage groups have significant difference compared with control group.
3.1.2. antibody tormation detection experiment:
Continuously to sample after 30 days, mouse cervical dislocation of the de- fiber sheep red blood cell (SRBC) after immune 5 days is put to death, spleen is taken out
It is placed in the plate for filling Hank's liquid, splenocyte suspension is made.Agarose is made into 1% aqueous solution, 45 DEG C of water-bath is boiled
30min, is mixed with the double Hanks liquid of equivalent pH 7.2-7.4, dispenses small test tube, every pipe 0.5m1, then adds into pipe 50 μ l
10%SRBC, splenocyte suspension 20u 1 do two Duplicate Samples, after rapid mixing, are poured on agarose thin layer slide, treat agar
After solidification, slide level is buckled and is placed on horse, is put into CO2gas incubator and is incubated 1.5h, complement is then added to glass
In horse groove, continue to incubate 1.5h, count hemolysis plaque number.As a result counted with variance analysis.
Antibody tormation detection experiment
● P < 0.05 (Analysis of variance) compared with control group
Through statistical analysis:Sample 60ml/kg dosage groups have significant difference compared with control group.
3.1.3 the measure of thymus index, spleen index:
Animal is continuously to sample after 30 days:Weighed per mouse, cervical dislocation is put to death, and takes thymus gland, spleen to weigh, thymus gland is calculated respectively
Index and spleen index.
Thymus index, spleen index
● P < 0.05 (Analysis of variance) compared with control group
Through statistical analysis:Each dosage group of sample is compared with control group, difference that there are no significant.
3.1.4. mouse carbonic clearance test
Mouse tail vein injection 1:The india ink of 3 dilutions, treats that prepared Chinese ink injects, timing immediately.Inject 2 after prepared Chinese ink, 10 points
Clock, takes the μ l of blood 20 from vena ophthalmica clump respectively, and is added into 2ml Na2CO3 solution, with spectrophotometer in 600nm wavelength
Place surveys OD values, and blank control is made with Na2CO3 solution.According to the weight of animals, liver weight, spleen re-computation phagocytic index, as a result with variance
Analysis is counted.
Carbonic clearance is tested
● P < 0.05 (Analysis of variance) compared with control group
Through statistical analysis:Sample 60ml/kg dosage groups have significant difference compared with control group.
3.1.5.ConA the mouse spleen lymphocyte conversion test induced
Animal is continuously to sample after 30 days, and cervical dislocation is put to death, and takes spleen, and splenocyte suspension is made, and adjustment cell concentration is
2*106/ml, cell suspension point holes is added into 24 well culture plates, per hole 1ml, a hole adds 50 μ l ConA liquid (equivalent to 5 μ
G/ml), 5%CO2,37 DEG C of culture 72h are put in another hole as control.Culture terminates preceding 4h, and supernatant is gently sucked per hole
0.7ml, adds the nutrient solutions of PRM 1640 that 0.7ml is free of calf serum, while adding MTT (5mg/ml) 50 μ g/ holes, continues
4h is cultivated, after culture terminates, 1ml acid isopropyl alcohol is added per hole, mixing is played in suction, is made after purple crystal is completely dissolved, with 570nm
Wavelength carries out colorimetric, is as a result analyzed with variance analysis.
The mouse spleen lymphocyte conversion test of ConA inductions
● P < 0.05 (Analysis of variance) compared with control group
Through statistical analysis:Sample 60ml/kg dosage groups have significant difference compared with control group.
3.1.6.DTH determine
Sensitization:Animal is continuously to sample after 30 days, per mouse belly unhairing, scope about 3*3cm, by the 1% μ l of DNFB solution 50
It is uniform to smear sensitization.
DTH generation and measure:After 5 days, uniformly it is applied to mouse right ear (two sides) with the μ l of 1%DNFB solution 10 and carries out
Attack.After 24 hours, cervical dislocation puts to death mouse, and diameter 8mm left and right auricle is removed with card punch, weighs and calculates swelling
Degree.
DHT measurement results
● P < 0.05 (Analysis of variance) compared with control group
Through statistical analysis:Sample 60ml/kg dosage groups have significant difference compared with control group.
3.1.7.NK cytoactive detection
Animal is continuously to sample after 30 days:Cervical dislocation is put to death, and takes spleen, splenocyte suspension (effector cell) is made, biography is taken
For rear 24h, YAC-1 cells add 1640 complete culture solutions, and adjustment cell concentration is 1*105/ml (target cell), takes target cell
With each 100 μ of effector cell effect targets ratio 50:1) U-shaped 96 well culture plate, is added, target cell Spontaneous release hole adds target cell and culture
Each 100 μ l of liquid, maximum release aperture adds target cell and each 100 μ l of 1%NP40, and above-mentioned items are all provided with three multiple holes, in 37 DEG C, 5%
Cultivated 4 hours in CO2 incubators, per in the well culture plate of hole 100 μ l horizontalizations bottom of Aspirate supernatant 96, while adding LDH matrix liquids
100 μ l, react 3min, and the μ l of HCL 30 that 1mol/L is added per hole are terminated, and OD value is determined at 490mm with ELIASA
(OD)。
NK cytoactive detections
● P < 0.05 (Analysis of variance) compared with control group
Through statistical analysis:Sample 60ml/kg dosage groups have significant difference compared with control group.
3.2.9 Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell experiment
20% chicken erythrocyte suspension 1ml is injected intraperitoneally per mouse continuously to sample after 30 days for animal, is spaced 30 minutes, and cervical vertebra takes off
Mortar is put to death, and is fixed on mouse plate, cuts off abdominal skin, and injecting normal saline 2ml rotates mouse plate 1 minute, suctions out abdominal cavity washing lotion
1ml, point drips and to incubate 30 minutes on 2 slides, 37 DEG C of incubators are wet, rinsed, dried with physiological saline, with 1:1 acetone methanol solution
Fixed, 4%Giemsa-phosphate buffer solution is dyed 3 minutes, then is dried with distilled water rinsing, uses oil mirror microscopy, calculating is gulped down
Bite % and phagocytic index.
Macrophage inflammatory protein-1α
● P < 0.05 (Analysis of variance) compared with control group
Through statistical analysis:Sample 60ml/kg dosage groups have significant difference compared with control group.
3.3 conclusion
After mouth gives sample 30 days, sample can substantially increase NK cytoactives, improve macrophages phagocytic capacity and body
Liquid is immunized and Cell-mediated Immunity, therefore sample has strengthen immunity function.
4th, opinion is summarized
To sum up, the sample Loaned swimming test is positive, and urea nitrogen and blood lactase acid result of the test are positive, and sample can substantially increase
NK cytoactives, raising macrophages phagocytic capacity and humoral immunity and Cell-mediated Immunity, according to function of health food
Evaluation criterion, the sample has the effect for alleviating physical fatigue and strengthen immunity.
It will be understood by those skilled in the art that the respective embodiments described above are to realize the specific embodiment of the present invention,
And in actual applications, can to it, various changes can be made in the form and details, without departing from the spirit and scope of the present invention.
Claims (9)
1. a kind of have the health-care food composition for alleviating physical fatigue and strengthen immunity function, it is characterised in that it is formulated
Including:
2. according to claim 1 have the health-care food composition for alleviating physical fatigue and strengthen immunity function, its
It is characterised by, its formula includes:
3. according to claim 1 have the health-care food composition for alleviating physical fatigue and strengthen immunity function, its
It is characterised by, its formula includes:
4. according to claim 1 have the health-care food composition for alleviating physical fatigue and strengthen immunity function, its
It is characterised by, its formula includes:
。
5. according to claim 1 have the health-care food composition for alleviating physical fatigue and strengthen immunity function, its
It is characterised by, the formula also includes:
Vitamin B1 0.06-0.09 parts by weight;
Vitamin B6 0.05-0.09 parts by weight.
6. combined according to the health food that physical fatigue and strengthen immunity function are alleviated in any described the having of claim 1-5
Thing, it is characterised in that the extracting method of the American Ginseng extract solution is as follows:
S1, American Ginseng pre-treatment:0.5~1.0cm tablet is cut into after American Ginseng is cleaned;
S2, extraction:100kg American Ginsengs are thrown in 3 tons of multi-function extractors, are added 10 times of amount (w/r) 50% edible ethanols, are heated back
3hr is flowed, extract solution is collected, residue adds the practical alcohol reflux 3hr of 10 times of amounts 50%, collects extract solution, residue adds 8 times of amounts
50% edible ethanol, backflow 1hr collects extract solution;
S3, sedimentation I:Merge No. three extract solutions, put in settling vat, settle 24 hours, take supernatant liquid filtering;
S4, concentration I:Decompression during above-mentioned filtered fluid is placed in recovery tower below 90 DEG C, is recycled to no alcohol taste;
S5, sedimentation II:Appropriate 95% ethanol is added in above-mentioned filtered fluid, and is stirred 1 hour, is settled 24 hours in settling vat
Afterwards by supernatant liquid filtering;
S6 concentrates II:Filter in recovery tower, below 90 DEG C, be recovered under reduced pressure above-mentioned.Be concentrated into relative density for 1.10~
1.20 soak thick paste.
S7, drying:Above-mentioned thick medicinal extract is put into clean Stainless Steel disk, in vacuum drying chamber, below 90 DEG C, reduced vacuum
Dry;
S8, crushing:Dried object is crushed with pulverizer, 100 mesh sieves is crossed and produces American ginseng extract solid powder.
7. according to claim 1 have the health-care food composition for alleviating physical fatigue and strengthen immunity function, its
It is characterised by, the extraction process of the Guarana extraction process is:
S1, Guarana seed is weighed in proportion, extracted 3 times, extracted 3 hours every time with the alcohol refluxs of 6~10 times of 90% concentration of amount,
Implication in proportion is herein:1 part of Guarana seed, 90% Ethanol Treatment is measured with 6-10 times;
S2, filtering, merging filtrate are recovered under reduced pressure after ethanol, are concentrated under reduced pressure into 60 DEG C, are obtained medicinal extract;
S3, medicinal extract is spray-dried, wherein, EAT is 150-180 DEG C, and leaving air temp is 60-90 DEG C, so as to obtain
Fine powder is spray-dried, Guarana extraction is produced.
8. the preparation containing health-care food composition as described in claim 1-5 is any, it is characterised in that said preparation be beverage,
Capsule, tablet, chewable tablets, granule, oral liquid, soft capsule, paste, stream juice and semi-fluid juice.
9. a kind of method of the preparation prepared described in claim 8, it is characterised in that this method is the common process of the formulation.
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