CN107227317A - A kind of cichory root extractive from fermentative and its preparation method and application - Google Patents
A kind of cichory root extractive from fermentative and its preparation method and application Download PDFInfo
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- CN107227317A CN107227317A CN201710661511.1A CN201710661511A CN107227317A CN 107227317 A CN107227317 A CN 107227317A CN 201710661511 A CN201710661511 A CN 201710661511A CN 107227317 A CN107227317 A CN 107227317A
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- fermentative
- cichory root
- bacillus pumilus
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- root extractive
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/20—Biochemical treatment
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/0003—Compounds of unspecified constitution defined by the chemical reaction for their preparation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
Abstract
The invention discloses a kind of cichory root extractive from fermentative and its preparation method and application.This method uses bacillus pumilus using cichory root as raw materialBacillus pumilusFermented, then be made after extracting aroma component in zymotic fluid.Described bacillus pumilusBacillus pumilusIt is stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center；Preserving number is CGMCC NO.3411；Preservation date：On November 05th, 2009.Contain a variety of aroma components in described cichory root extractive from fermentative, be equably sprayed at when using as bed material on pipe tobacco, addition is 5~20g/ casees.Perfuming result of the test of smokeing panel test shows that the extractive from fermentative and cigarette perfume more harmony cover miscellaneous gas, reduce excitant, increase cigarette flavor matter, improve comfort level, be effectively improved cigarette quality.
Description
Technical field
The present invention relates to a kind of extractive from fermentative, and in particular to a kind of cichory root extractive from fermentative, meanwhile, the present invention is also related to
And the preparation method and application of cichory root extractive from fermentative, belong to cigarette additive technical field.
Background technology
Essence and flavoring agent plays key effect to the taste of cigarette product.Current most spices is all by chemistry conjunction
Obtained into method, but not only cost is high, synthesis is difficult for the spices of chemical synthesis, and one can be caused to be fixed to environment and health
Ring.There is extraction cost height, the problems such as raw material is limited again in the spices directly extracted by natural animal-plant.Utilize microbial technique
The spices that fermenting plant material is prepared belongs to natural perfume material rank, and while product quality is improved, can save
Resource, reduces cost.
There is bacillus pumilus to improve the research of quality of tobacco at present, tobacco leaf fermented using bacillus pumilus,
However, it still has excitement to throat, it can not also increase flue gas sweet tea sense, effect is not ideal enough.
Witloof is a kind of brushy herbaceos perennial, root meat, short and thick.In Yunnan Province of China, Sichuan（Chengdu）
And there is introducing and planting in Guangdong etc..Witloof is medical and edible dual purpose plant, and the modulated romaine lettuce of chicory leaf, root contains synanthrin and aromatic substance,
With clearing heat and detoxicating, inducing diuresis to remove edema, the effects such as stomach invigorating, coffee, beverage, tea, health products, food, Chinese medicine are currently widely used for
In.But fermentation extraction in the prior art, is carried out to the nutriment and fragrance of witloof using microorganism, and it is fragrant as flavouring essence for tobacco
There is presently no relevant report for the method that material is applied in cigarette.
The content of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a kind of cichory root extractive from fermentative, applied
In cigarette, to lift the flavouring essence quality of cigarette product.
It is a further object of the present invention to provide a kind of method for preparing the witloof cichory root extractive from fermentative.
The present invention also aims to provide application of the cichory root extractive from fermentative in production of cigarettes.
The purpose of the present invention is achieved by following technical proposals.
* unless otherwise indicated, the percentage employed in the present invention is mass percent.
A kind of cichory root tobacco aromaticss, it is characterised in that：After cichory root is crushed, bacillus pumilus is usedBacillus pumilusFermented, fermentation gains are required tobacco aromaticss；Described bacillus pumilusBacillus pumilusIt is stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center；Preserving number is CGMCC
NO.3411；Preservation date：On November 05th, 2009.Bacillus pumilusBacillus pumilusFrom flue-cured tobacco surface separation and
.
The preparation method of above-mentioned cichory root extractive from fermentative, it is characterised in that：Comprise the following steps：
（1）Bacillus pumilus CGMCC NO.3411 activation cultures are taken, bacillus pumilus activation bacterium solution is obtained；Actication of culture
Condition of culture is 30 DEG C~38 DEG C, 100~180r/min shaken cultivations, and incubation time is 6~24h；
（2）After cichory root is crushed, the distilled water of 10~20 times of amounts is added, sterilizing, which is made, treats fermented sample；
（3）The bacterium solution activated is inoculated in and treated in fermented sample, inoculum concentration is 0.5%-2.5%；Fermented and cultured bar after inoculation
Part is with activation culture condition；Incubation time is 6~24h；
（4）Zymotic fluid takes supernatant after filtering, centrifugation, is concentrated under reduced pressure into medicinal extract under the conditions of temperature is 45 DEG C~65 DEG C, i.e.,
Cichory root extractive from fermentative for needed for.
Application of the above-mentioned cichory root extractive from fermentative in production of cigarettes, it is characterised in that：In cigarette primary processing feeding stage,
Described cichory root extractive from fermentative is equably sprayed on pipe tobacco, addition is 5~20g/ case pipe tobaccos.
Compared with prior art, the present invention has advantages below：
（1）, the present invention initiatively using cichory root as raw material, utilize bacillus pumilusBacillus pumilusFermented
Essence spice for cigarette is prepared, and is added in bed material form in cigarette.
（2）, be made after spices, the active ingredient and its distinctive fragrance in cichory root with pipe tobacco it is compound after, can
Effective increasing smoke concentration, increases sweet sense, and reducing throat stimulates.
（3）, present invention process it is simple, operation is easy, and cost is low, suitable for popularization and application, to promoting cigarette industry health to send out
Exhibition has positive effect.
Embodiment
It is clearly and completely described below in conjunction with the technical scheme in embodiment, it is clear that described embodiment is only
Only it is the example to a part of example of the invention, rather than whole.Based on the embodiment in the present invention, ordinary skill people
The every other embodiment that member is obtained under the premise of creative work is not paid, belongs to the scope of protection of the invention.Should
Work as understanding, the specific embodiments described herein are merely illustrative of the present invention, be not intended to limit the present invention.In embodiment not
Particular technique or condition person are indicated, is entered according to the technology or condition described by document in the art or according to product description
OK.Agents useful for same or the unreceipted production firm person of instrument, being can be by buying the conventional products obtained.LB used
(Luria-Bertani) culture medium：1% peptone, 1% sodium chloride, 0. 5% yeast extract.
Embodiment 1
The preparation method of the cichory root extractive from fermentative of the present embodiment, comprises the following steps：
（1）Take bacillus pumilus CGMCC NO.3411 to carry out activation culture in LB fluid nutrient mediums, obtain short and small gemma bar
Bacterium activates bacterium solution；Strain activation and culture condition is 37 DEG C, 120r/min shaken cultivations, and incubation time is 12h；
（2）After cichory root is crushed, 100g cichory root powder is obtained, the distilled water of 15 times of amounts is added, sterilized 30 points at 121 DEG C
Clock, is made and treats fermented sample；
（3）By step（1）The middle bacterium solution activated is inoculated in step（2）In treat in fermented sample, inoculum concentration is 1%；After inoculation
The shaken cultivation 12h under the conditions of 37 DEG C, 120 r/min；
（4）Zymotic fluid takes supernatant after filtering, centrifugation, medicinal extract is concentrated under reduced pressure under the conditions of temperature is 50 DEG C, needed for being
Cichory root extractive from fermentative, preserve it is stand-by.
（5）To step（4）The cichory root extractive from fermentative of gained carries out aroma component detection, testing result using GC-MS
Show, the higher flavor matter of content mainly has palmitic acid, furfural, linoleic acid, 3 methylvaleric acid, 2- second in the fermentation gains
Acyl group furans, caproic acid, 2- acetyl pyrroles, furfuryl alcohol, 2- methyltetrahydrofuran -3- ketone, 2- cyclopentene -1,4- diketone, phenylacetaldehyde,
4- vinyl guaiacols, pyridine, eugenol, 2- acetylpyridines, methyl hexadecanoate, pyrazine, vanillic aldehyde etc..
（6）By step（4）The cichory root extractive from fermentative of gained is equably sprayed on pipe tobacco as bed material, and addition is
8g/ casees, and using the tobacco leaf without spices as control.The pipe tobacco for the treatment of group and control group is put into climatic chamber inner equilibrium
After 24h, cigarette, sample and the equal random number of control is made, the group of smokeing panel test being made up of the expert that smokes panel test carries out sensory evaluating smoking.Perfuming
Result of the test of smokeing panel test shows that the perfume additive harmony cigarette perfume enriches flue gas concentration, and reducing throat stimulates, the soft exquisiteness of flue gas,
Sweet sense increase.
Embodiment 2
The preparation method of the cichory root extractive from fermentative of the present embodiment, comprises the following steps：
（1）Take bacillus pumilus CGMCC NO.3411 to carry out activation culture in LB fluid nutrient mediums, obtain short and small gemma bar
Bacterium activates bacterium solution；Strain activation and culture condition is 37 DEG C, 120r/min shaken cultivations, and incubation time is 12h；
（2）After cichory root is crushed, 100g cichory root powder is obtained, the distilled water of 20 times of amounts is added, sterilized 30 points at 121 DEG C
Clock, is made and treats fermented sample；
（3）By step（1）The middle bacterium solution activated is inoculated in step（2）In treat in fermented sample, inoculum concentration is 2%；After inoculation
The shaken cultivation 12h under the conditions of 37 DEG C, 120 r/min；
（4）Zymotic fluid takes supernatant after filtering, centrifugation, medicinal extract is concentrated under reduced pressure under the conditions of temperature is 50 DEG C, needed for being
Cichory root extractive from fermentative, preserve it is stand-by.
（5）To step（4）The cichory root extractive from fermentative of gained carries out aroma component detection, testing result table using GCMS
Bright, the higher flavor matter of content mainly has palmitic acid, furfural, linoleic acid, 3 methylvaleric acid, 2- acetyl in the fermentation gains
Base furans, caproic acid, 2- acetyl pyrroles, furfuryl alcohol, 2- methyltetrahydrofuran -3- ketone, 2- cyclopentene -1,4- diketone, phenylacetaldehyde, 4-
Vinyl guaiacol, pyridine, eugenol, 2- acetylpyridines, methyl hexadecanoate, pyrazine, vanillic aldehyde etc..
（6）By step（4）The cichory root extractive from fermentative of gained is equably sprayed on pipe tobacco as bed material, and addition is
8g/ casees, and using the tobacco leaf without spices as control.The pipe tobacco for the treatment of group and control group is put into climatic chamber inner equilibrium
After 24h, cigarette, sample and the equal random number of control is made, the group of smokeing panel test being made up of the expert that smokes panel test carries out sensory evaluating smoking.Perfuming
Result of the test of smokeing panel test shows that the perfume additive harmony cigarette perfume, flue gas concentration increase, lingual surface residual is few, and pleasant impression is comfortably clean.
Embodiment 3
The preparation method of the cichory root extractive from fermentative of the present embodiment, comprises the following steps：
（1）Take bacillus pumilus CGMCC NO.3411 to carry out activation culture in LB fluid nutrient mediums, obtain short and small gemma bar
Bacterium activates bacterium solution；Strain activation and culture condition is 33 DEG C, 160r/min shaken cultivations, and incubation time is 18h；
（2）After cichory root is crushed, 100g cichory root powder is obtained, the distilled water of 15 times of amounts is added, sterilized 30 points at 121 DEG C
Clock, is made and treats fermented sample；
（3）By step（1）The middle bacterium solution activated is inoculated in step（2）In treat in fermented sample, inoculum concentration is 1%；After inoculation
The shaken cultivation 18h under the conditions of 37 DEG C, 160 r/min；
（4）Zymotic fluid takes supernatant after filtering, centrifugation, medicinal extract is concentrated under reduced pressure under the conditions of temperature is 50 DEG C, needed for being
Cichory root extractive from fermentative, preserve it is stand-by.
（5）To step（4）The cichory root extractive from fermentative of gained carries out aroma component detection, testing result table using GCMS
Bright, the higher flavor matter of content mainly has palmitic acid, furfural, linoleic acid, 3 methylvaleric acid, 2- acetyl in the fermentation gains
Base furans, caproic acid, 2- acetyl pyrroles, furfuryl alcohol, 2- methyltetrahydrofuran -3- ketone, 2- cyclopentene -1,4- diketone, phenylacetaldehyde, 4-
Vinyl guaiacol, pyridine, eugenol, 2- acetylpyridines, methyl hexadecanoate, pyrazine, vanillic aldehyde etc..
（6）By step（4）The cichory root extractive from fermentative of gained is equably sprayed on pipe tobacco as bed material, and addition is
8g/ casees, and using the tobacco leaf without spices as control.The pipe tobacco for the treatment of group and control group is put into climatic chamber inner equilibrium
After 24h, cigarette, sample and the equal random number of control is made, the group of smokeing panel test being made up of the expert that smokes panel test carries out sensory evaluating smoking.Perfuming
Result of the test of smokeing panel test shows that the perfume additive harmony cigarette perfume, fine and smooth alcohol and flue gas enrich flue gas concentration, increase sweet tea sense, reduce
Residual, improves cigarette odor-absorbing.
Embodiment 4
The preparation method of the cichory root extractive from fermentative of the present embodiment, comprises the following steps：
（1）Take bacillus pumilus CGMCC NO.3411 to carry out activation culture in LB fluid nutrient mediums, obtain short and small gemma bar
Bacterium activates bacterium solution；Strain activation and culture condition is 33 DEG C, 160r/min shaken cultivations, and incubation time is 18h；
（2）After cichory root is crushed, 100g cichory root powder is obtained, the distilled water of 15 times of amounts is added, sterilized 30 points at 121 DEG C
Clock, is made and treats fermented sample；
（3）By step（1）The middle bacterium solution activated is inoculated in step（2）In treat in fermented sample, inoculum concentration is 2%；After inoculation
The shaken cultivation 18h under the conditions of 33 DEG C, 160 r/min；
（4）Zymotic fluid takes supernatant after filtering, centrifugation, medicinal extract is concentrated under reduced pressure under the conditions of temperature is 50 DEG C, needed for being
Cichory root extractive from fermentative, preserve it is stand-by.
（5）To step（4）The cichory root extractive from fermentative of gained carries out aroma component detection, testing result table using GCMS
Bright, the higher flavor matter of content mainly has palmitic acid, furfural, linoleic acid, 3 methylvaleric acid, 2- acetyl in the fermentation gains
Base furans, caproic acid, 2- acetyl pyrroles, furfuryl alcohol, 2- methyltetrahydrofuran -3- ketone, 2- cyclopentene -1,4- diketone, phenylacetaldehyde, 4-
Vinyl guaiacol, pyridine, eugenol, 2- acetylpyridines, methyl hexadecanoate, pyrazine, vanillic aldehyde etc..
（6）By step（4）The cichory root extractive from fermentative of gained is equably sprayed on pipe tobacco as bed material, and addition is
8g/ casees, and using the tobacco leaf without spices as control.The pipe tobacco for the treatment of group and control group is put into climatic chamber inner equilibrium
After 24h, cigarette, sample and the equal random number of control is made, the group of smokeing panel test being made up of the expert that smokes panel test carries out sensory evaluating smoking.Perfuming
Result of the test of smokeing panel test shows that the perfume additive harmony cigarette perfume, increasing smoke concentration increases back sweet tea sense, and soft fine and smooth flue gas is remaining
Taste is totally comfortable.
Embodiment 5
The preparation method of the cichory root extractive from fermentative of the present embodiment, comprises the following steps：
（1）Take bacillus pumilus CGMCC NO.3411 to carry out activation culture in LB fluid nutrient mediums, obtain short and small gemma bar
Bacterium activates bacterium solution；Strain activation and culture condition is 30 DEG C, 160r/min shaken cultivations, and incubation time is 24h；
（2）After cichory root is crushed, 100g cichory root powder is obtained, the distilled water of 15 times of amounts is added, sterilized 30 points at 121 DEG C
Clock, is made and treats fermented sample；
（3）By step（1）The middle bacterium solution activated is inoculated in step（2）In treat in fermented sample, inoculum concentration is 1%；After inoculation
The shaken cultivation 24h under the conditions of 30 DEG C, 160 r/min；
（4）Zymotic fluid takes supernatant after filtering, centrifugation, medicinal extract is concentrated under reduced pressure under the conditions of temperature is 50 DEG C, needed for being
Cichory root extractive from fermentative, preserve it is stand-by.
（5）To step（4）The cichory root extractive from fermentative of gained carries out aroma component detection, testing result table using GCMS
Bright, the higher flavor matter of content mainly has palmitic acid, furfural, linoleic acid, 3 methylvaleric acid, 2- acetyl in the fermentation gains
Base furans, caproic acid, 2- acetyl pyrroles, furfuryl alcohol, 2- methyltetrahydrofuran -3- ketone, 2- cyclopentene -1,4- diketone, phenylacetaldehyde, 4-
Vinyl guaiacol, pyridine, eugenol, 2- acetylpyridines, methyl hexadecanoate, pyrazine, vanillic aldehyde etc..
（6）By step（4）The cichory root extractive from fermentative of gained is equably sprayed on pipe tobacco as bed material, and addition is
8g/ casees, and using the tobacco leaf without spices as control.The pipe tobacco for the treatment of group and control group is put into climatic chamber inner equilibrium
After 24h, cigarette, sample and the equal random number of control is made, the group of smokeing panel test being made up of the expert that smokes panel test carries out sensory evaluating smoking.Perfuming
Result of the test of smokeing panel test shows that the perfume additive harmony cigarette perfume, increasing smoke concentration, soft fine and smooth flue gas, pleasant impression is totally comfortable,
Promote the production of body fluid Hui Tian.
Embodiment 6
The preparation method of the cichory root extractive from fermentative of the present embodiment, comprises the following steps：
（1）Take bacillus pumilus CGMCC NO.3411 to carry out activation culture in LB fluid nutrient mediums, obtain short and small gemma bar
Bacterium activates bacterium solution；Strain activation and culture condition is 30 DEG C, 160r/min shaken cultivations, and incubation time is 24h；
（2）After cichory root is crushed, 100g cichory root powder is obtained, the distilled water of 20 times of amounts is added, sterilized 30 points at 121 DEG C
Clock, is made and treats fermented sample；
（3）By step（1）The middle bacterium solution activated is inoculated in step（2）In treat in fermented sample, inoculum concentration is 2%；After inoculation
The shaken cultivation 24h under the conditions of 30 DEG C, 160 r/min；
（4）Zymotic fluid takes supernatant after filtering, centrifugation, medicinal extract is concentrated under reduced pressure under the conditions of temperature is 50 DEG C, needed for being
Cichory root extractive from fermentative, preserve it is stand-by.
（5）To step（4）The cichory root extractive from fermentative of gained carries out aroma component detection, testing result table using GCMS
Bright, the higher flavor matter of content mainly has palmitic acid, furfural, linoleic acid, 3 methylvaleric acid, 2- acetyl in the fermentation gains
Base furans, caproic acid, 2- acetyl pyrroles, furfuryl alcohol, 2- methyltetrahydrofuran -3- ketone, 2- cyclopentene -1,4- diketone, phenylacetaldehyde, 4-
Vinyl guaiacol, pyridine, eugenol, 2- acetylpyridines, methyl hexadecanoate, pyrazine, vanillic aldehyde etc..
（6）By step（4）The cichory root extractive from fermentative of gained is equably sprayed on pipe tobacco as bed material, and addition is
8g/ casees, and using the tobacco leaf without spices as control.The pipe tobacco for the treatment of group and control group is put into climatic chamber inner equilibrium
After 24h, cigarette, sample and the equal random number of control is made, the group of smokeing panel test being made up of the expert that smokes panel test carries out sensory evaluating smoking.Perfuming
Result of the test of smokeing panel test shows that the perfume additive harmony cigarette perfume, increasing smoke concentration assigns cigarette and naturally bakes fragrant and sweet gas, fragrant
Gas is fine and smooth, fine and soft long, and pleasant impression is clean, and promote the production of body fluid Hui Tian.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God is with principle, and any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.
Claims (3)
1. a kind of cichory root extractive from fermentative, it is characterised in that：Using cichory root as raw material, fermented, fermented with bacillus pumilus
Gains produce product after filtering, concentration；Described bacillus pumilusBacillus pumilusIt is stored in Chinese micro- life
Thing culture presevation administration committee common micro-organisms center；Preserving number is CGMCC NO.3411；Preservation date：In November, 2009
05 day.
2. the preparation method of cichory root extractive from fermentative described in claim 1, it is characterised in that：Comprise the following steps：
（1）Bacillus pumilus CGMCC NO.3411 activation cultures are taken, bacillus pumilus activation bacterium solution is obtained；Actication of culture
Condition of culture is 30 DEG C~38 DEG C, 100~180r/min shaken cultivations, and incubation time is 6~24h；
（2）After cichory root is crushed, the distilled water of 10~20 times of amounts is added, sterilizing, which is made, treats fermented sample；
（3）By step（1）The middle bacterium solution activated, which is inoculated in, to be treated in fermented sample, and inoculum concentration is 0.5%-2.5%；Hair after inoculation
Ferment condition of culture is with activation culture condition；Incubation time is 6~24h；
（4）Zymotic fluid takes supernatant after filtering, centrifugation, is concentrated under reduced pressure into medicinal extract under the conditions of temperature is 45 DEG C~65 DEG C, i.e.,
Cichory root extractive from fermentative for needed for.
3. application of the cichory root extractive from fermentative in production of cigarettes described in claim 1, it is characterised in that：In cigarette primary processing plus
In the material stage, described cichory root extractive from fermentative is equably sprayed on pipe tobacco, addition is 5~20g/ case pipe tobaccos.
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