CN107224339A - One stud ram electro-ejaculation method - Google Patents
One stud ram electro-ejaculation method Download PDFInfo
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- CN107224339A CN107224339A CN201710381079.0A CN201710381079A CN107224339A CN 107224339 A CN107224339 A CN 107224339A CN 201710381079 A CN201710381079 A CN 201710381079A CN 107224339 A CN107224339 A CN 107224339A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
- A61D19/021—Apparatus for collecting seminal fluids; Artificial vaginas
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- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Reproductive Health (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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- Orthopedics, Nursing, And Contraception (AREA)
- Percussion Or Vibration Massage (AREA)
Abstract
The present invention is that the present invention discloses a stud ram electro-ejaculation method, belongs to livestock and poultry cultivation technical field.Described ram electro-ejaculation method is concretely comprised the following steps：1. Baoding of ram；2. cropping around penis；3. ram penis and testis are wiped successively with potassium permanganate solution and physiological saline；4. ram glans penis is exported and is fastened with the sterile gauze bar of wetting from ram foreskin by human assistance；5. carry out rectal distention stimulation using electro-ejaculation instrument and massage；6. collect seminal fluid.The present invention ram electro-ejaculation method it is simple to operate, quick, repeatable, for Seed practical value it is high herd or drylot feeding ram, the inaccessible ram of wild nature strong man, the ram without mounting ability or standby male young sheep can be used electrical stimulation method smoothly collect seminal fluid.
Description
Technical field
The invention belongs to livestock and poultry cultivation technical field, and in particular to a stud ram electro-ejaculation method.
Background technology
With the fast development of modern Mutton Sheep Industry, specialization, scale, intensive and standardization sheep raising production meet the tendency of
And give birth to.Technology of artificial insemination is the breeding management means gradually emerged during modern sheep raising produces.Artificial insemination not only improves outstanding
The breeding efficiency of stud ram, obtains higher economic benefit, moreover it is possible to strengthen the preservation of Breeds resources so that some improved seeds can
To continue development.The semen collection of high-quality is the precondition of technology of artificial insemination, at present, and the semen collection method of ram mainly has
Pseudopyloric metaplasia and electrostimulation.Pseudopyloric metaplasia requirement before stud ram artificial semenpicking to carrying out good instruction, in addition it is also necessary to which heat is female
The cooperation of sheep, previous work is relatively complicated.Separately there are some rams or the inaccessible ram of wild nature strong man without mounting ability, tame and docile
Change instruction work more difficult, artificial semenpicking difficulty.Electro-ejaculation method is proven, and can smoothly obtain ram seminal fluid.But mesh
Preceding visible report is to use that ram is implemented to carry out electro-ejaculation after anaesthetizing mostly, and suitable security risk is caused to stud ram.
The content of the invention
It is an object of the invention to provide a stud ram electro-ejaculation method, described semen collection method is applied to herd or give up
Raise the semen collection of ram, the inaccessible ram of wild nature strong man, the ram without mounting ability or standby male young sheep.Described
Semen collection method can exempt instruction domestication work of the Pseudopyloric metaplasia semen collection early stage to stud ram.Described semen collection method is without to ram
Anaesthetized, security risk and the influence to seminal fluid caused by reduction anesthesia.Described semen collection method is noninvasive during semen collection
Wound, obvious pain is not resulted in ram, simple to operate, quick, repeatable simple to operate, quick, repeatable.
The purpose of the present invention is realized by following technical schemes：One stud ram electro-ejaculation method, it is characterised in that
The concrete operation step of methods described is as follows：
(1) ram is exposed testis and urethral orifice by work；
(2) hair at ram belly and penis is trimmed, successively with 0.1%-0.2% potassium permanganate solution and physiology salt
Water wipes the dunghill of ram belly and urethral orifice, dries；
(3) electro-ejaculation：Hold with a firm grip respectively the penis end of testis root and the foreskin chamber of urethral orifice, both hands simultaneously to
Contraction is had an effect, and ram glans penis is exported from ram foreskin, and is fixed with the sterile gauze bar of wetting, prevents retraction foreskin
It is interior；Fixed glans penis, keeps glans penis and processus urethrae outside foreskin chamber, prepares to collect seminal fluid；By electro-ejaculation instrument
Electrode bar inserts ram rectum, while massaging testis and regulation electro-ejaculation instrument；
(4) connect electro-ejaculation instrument power supply, stimulated, initial voltage be 0.3V to 0.4V, electric current 8mA to 10mA,
Each conduction time is 5 to 6s, and interval time, usual 3 to 10 circulations can collect seminal fluid for 8 to 10s；Such as at 10
Seminal fluid is not yet collected after stimulation cycle can bring the voltage up to 0.6V to 0.8V, and electric current 15 is to 20mA, each conduction time
For 5 to 6s, interval time is that 15 to 20s, usual 5 to 8 circulations can collect seminal fluid, and accumulative stimulation time is no more than
10min；
(5) while step (4) electro-ejaculation, the personnel for collecting seminal fluid take advantage of a situation erection when being powered stimulation
Penis imports sterile centrifugation tube and seminal fluid is inscribed, while the sterile gauze bar of fixed glans penis is suitably loosened；The people stimulated
Member moderately massages testis and spermatic cord when powering off interval with hand, is massaged while stimulating electrode is touched in rectum to dorsal part；
(6) need seminal fluid being diluted according to produce reality or constant temperature is preserved, unbinding cloth.
Further, when the step (3) if the electrode sense of touch of middle insertion ram rectum first should pierce electricity to when having excrement
The electrode bar for swashing semen collection instrument is extracted, and excrement is removed totally, then again again by the electricity of electro-ejaculation instrument with sterile gauze
The rectum that pole rod is inserted into ram is stimulated.
Further, portion is recommended for the waist of ram in the position that electrode bar and massage are stimulated in the step (5).
Further, described ram electro-ejaculation method is applied to sheep and goat.
Further, if the accumulative ram for stimulating more than 10min not ejaculate, assert that this electro-ejaculation fails, rest
Repeat within 1-2 days；Ram semen collection frequency is advisable for 2-3 times weekly, and semen collection ram is supplemented the nutrients.
The beneficial effects of the invention are as follows：
The present invention ram electro-ejaculation method it is simple to operate, quick, repeatable, for Seed practical value it is high herd or
Electro photoluminescence can be used in drylot feeding ram, the inaccessible ram of wild nature strong man, the ram without mounting ability or standby male young sheep
Method smoothly collects seminal fluid.The ram seminal fluid that methods described is obtained can be used for artificial insemination, semen cryopreservation, breeding physiology
Learn research, progeny testing or import and export test alive quarantine etc., without implementing to anaesthetize to ram, can reduce to greatest extent to kind of a public affairs
The influence of sheep.
Embodiment
Present disclosure is further described below in conjunction with currently preferred specific embodiment.Example is only
For explaining the present invention, the scope of the present invention is not intended to limit.
Specific embodiment, selects 8 adult healthy Hainan black goat stud rams, and electro-ejaculation instrument used is
BATERSUARG times of special double MDW-1 types, stimulating electrodeConcrete operation step is as follows：
(1) 3 personnel implements binding of lying on one's side to stud ram, wherein 2 personnel are public with coarse cloth bar Baoding wide 2cm respectively
Hoof before and after sheep, 1 personnel Baoding stud ram neck and back.
(2) hair at stud ram belly and penis is cut short with cropping scissors, first wiped with 0.1% potassium permanganate solution
The dunghill of ram belly and urethral orifice is wiped, then is wiped with physiological saline.
(3) 2 personnel implement electro-ejaculation, and a people is located at stud ram veutro, the penis of a tight testis root of holding
End, another hand holds the foreskin chamber of urethral orifice, and both hands to contraction simultaneously to having an effect, and human assistance is by ram glans penis from public affairs
Export, and fixed with the sterile gauze bar of wetting in sheep foreskin, prevented in retraction foreskin.This latter hand connects by sterile centrifugation tube
Seminal fluid, another hand fixes glans penis with the wide sterile gauze bars of 1cm, keeps glans penis and processus urethrae outside foreskin chamber,
Collect seminal fluid.Another people inserts electrodes into stud ram rectum depth 15cm positioned at stud ram afterbody, and is responsible for testis massage and electricity
Stimulate semen collection instrument switch-mode regulation.
(4) electro-ejaculation instrument power supply is connected, is stimulated, initial voltage is 0.3V, electric current 10mA, each conduction time
For 5s, interval time is 10s, and usual 3-10 circulation can collect seminal fluid.To not yet being collected after 10 stimulation cycles
The Hainan black goat stud ram of seminal fluid, brings the voltage up to 0.6V, electric current 15-20mA, each conduction time is 5s, interval time
For 20s, usual 5-8 circulation can collect seminal fluid.
(5) while step (4) electro-ejaculation, the personnel for collecting seminal fluid take advantage of a situation erection when being powered stimulation
Penis imports sterile centrifugation tube and seminal fluid is inscribed, while the sterile gauze bar of fixed glans penis is suitably loosened, is easy to row's essence.Carry out
The personnel of stimulation moderately massage testis and spermatic cord when powering off interval with hand, while stimulating electrode is pressed in rectum to dorsal part touching
Rub, also can the row's of stimulation essence.
(6) after ram ejaculation, the sterile gauze bar of fixed glans penis is withdrawn from erect penile condition, and by electrode from straight
Extracted in intestines, carry out disinfection wiping to anus, seminal fluid is diluted on request or constant temperature is preserved, unbinding cloth.
Hainan black goat stud ram seminal fluid on-site measurement ejaculation amount that above method semen collection is arrived, sperm concentration, sperm are lived
Power, the results are shown in Table 1.
| Ram number | Ejaculation amount | Cloud is moved | Sperm concentration | Sperm motility |
| 1# | 1.2ml | √ | +++ | ＞ 90% |
| 2# | 1.0ml | √ | ++ | ＞ 90% |
| 3# | 1.5ml | √ | +++ | ＞ 90% |
| 4# | 2.0ml | √ | + | ＞ 60% |
| 5# | 2.0ml | √ | ++ | ＞ 80% |
| 6# | 1.0ml | √ | + | ＞ 80% |
| 7# | 1.2ml | √ | ++ | ＞ 90% |
| 8# | 1.5ml | √ | +++ | ＞ 90% |
The Hainan black goat stud ram electro-ejaculation result of table 1
As shown in Table 1, ram electro-ejaculation method of the present invention, successful acquisition to Hainan more than 1ml volumes
Black goat stud ram seminal fluid, and by primary microscopy and semen quality evaluation, except two ram sperm density relatively low (density+),
One ram sperm vigor is relatively low (vigor 60%-70%), and remaining seminal fluid reaches the mark of artificial insemination or semen cryopreservation
It is accurate.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.
Claims (5)
1. a stud ram electro-ejaculation method, it is characterised in that the concrete operation step of methods described is as follows：
(1) ram is exposed testis and urethral orifice by work；
(2) hair at ram belly and penis is trimmed, is wiped successively with 0.1%-0.2% potassium permanganate solution and physiological saline
The dunghill of ram belly and urethral orifice is wiped, is dried；
(3) electro-ejaculation：Hold with a firm grip respectively the penis end of testis root and the foreskin chamber of urethral orifice, both hands are simultaneously to contraction
Have an effect, ram glans penis is exported from ram foreskin, and fixed with the sterile gauze bar of wetting, prevented in retraction foreskin；Gu
Determine glans penis, keep glans penis and processus urethrae outside foreskin chamber, prepare to collect seminal fluid；By the electrode bar of electro-ejaculation instrument
Ram rectum is inserted, while massaging testis and regulation electro-ejaculation instrument；
(4) connect electro-ejaculation instrument power supply, stimulated, initial voltage be 0.3V to 0.4V, electric current 8mA to 10mA, every time
Conduction time is 5 to 6s, and interval time, usual 3 to 10 circulations can collect seminal fluid for 8 to 10s；Such as followed in 10 stimulations
Seminal fluid is not yet collected after ring can bring the voltage up to 0.6V to 0.8V, electric current 15 to 20mA, each conduction time be 5 to
6s, interval time, usual 5 to 8 circulations can collect seminal fluid for 15 to 20s, add up stimulation time and be no more than 10min；
(5) while step (4) electro-ejaculation, the personnel for collecting seminal fluid take advantage of a situation the penis of erection when being powered stimulation
Import sterile centrifugation tube and seminal fluid is inscribed, while the sterile gauze bar of fixed glans penis is suitably loosened；The personnel stimulated exist
Testis and spermatic cord are moderately massaged with hand during power-off interval, is massaged while stimulating electrode is touched in rectum to dorsal part；
(6) need seminal fluid being diluted according to produce reality or constant temperature is preserved, unbinding cloth.
2. ram electro-ejaculation method according to claim 1, it is characterised in that when insertion is public in the step (3)
If the electrode sense of touch of sheep rectum is to when having excrement, first the electrode bar of electro-ejaculation instrument should be extracted, and by the sterile yarn of excrement
Cloth is removed totally, and the rectum that the electrode bar of electro-ejaculation instrument then is inserted into ram again again is stimulated.
3. ram electro-ejaculation method according to claim 1, it is characterised in that in the step (5) electrode bar and
Portion is recommended for the waist of ram in the position that massage is stimulated.
4. ram electro-ejaculation method according to claim 1, it is characterised in that described ram electro-ejaculation side
Method is applied to sheep and goat.
5. ram electro-ejaculation method according to claim 1, it is characterised in that if accumulative stimulate more than 10min not
The ram of ejaculation, assert that this electro-ejaculation fails, rest is repeated for 1-2 days；Ram semen collection frequency is is for 2-3 times weekly
Preferably, and to semen collection ram supplement the nutrients.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710381079.0A CN107224339A (en) | 2017-05-25 | 2017-05-25 | One stud ram electro-ejaculation method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710381079.0A CN107224339A (en) | 2017-05-25 | 2017-05-25 | One stud ram electro-ejaculation method |
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| Publication Number | Publication Date |
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| CN107224339A true CN107224339A (en) | 2017-10-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710381079.0A Pending CN107224339A (en) | 2017-05-25 | 2017-05-25 | One stud ram electro-ejaculation method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113081377A (en) * | 2021-04-09 | 2021-07-09 | 四川省龙日种畜场 | Yak electrical stimulation semen collection method |
| CN115381589A (en) * | 2022-10-10 | 2022-11-25 | 杭州野生动物世界有限公司 | Semen collection method for tiger south China through electrical stimulation |
-
2017
- 2017-05-25 CN CN201710381079.0A patent/CN107224339A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113081377A (en) * | 2021-04-09 | 2021-07-09 | 四川省龙日种畜场 | Yak electrical stimulation semen collection method |
| CN115381589A (en) * | 2022-10-10 | 2022-11-25 | 杭州野生动物世界有限公司 | Semen collection method for tiger south China through electrical stimulation |
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Application publication date: 20171003 |
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