CN113081377A - Yak electrical stimulation semen collection method - Google Patents
Yak electrical stimulation semen collection method Download PDFInfo
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- CN113081377A CN113081377A CN202110387621.XA CN202110387621A CN113081377A CN 113081377 A CN113081377 A CN 113081377A CN 202110387621 A CN202110387621 A CN 202110387621A CN 113081377 A CN113081377 A CN 113081377A
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- 210000000582 semen Anatomy 0.000 title claims abstract description 113
- 230000000638 stimulation Effects 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 40
- 210000000664 rectum Anatomy 0.000 claims abstract description 12
- 210000004706 scrotum Anatomy 0.000 claims abstract description 12
- 230000004936 stimulating effect Effects 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000004140 cleaning Methods 0.000 claims abstract description 8
- 230000001105 regulatory effect Effects 0.000 claims abstract description 8
- 230000000630 rising effect Effects 0.000 claims abstract description 8
- 210000005036 nerve Anatomy 0.000 claims abstract description 6
- 238000003780 insertion Methods 0.000 claims abstract description 5
- 230000037431 insertion Effects 0.000 claims abstract description 5
- 230000001276 controlling effect Effects 0.000 claims abstract description 4
- 239000012153 distilled water Substances 0.000 claims abstract description 4
- 239000000344 soap Substances 0.000 claims abstract description 4
- 229940099259 vaseline Drugs 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 12
- 210000003899 penis Anatomy 0.000 claims description 7
- 230000003187 abdominal effect Effects 0.000 claims description 5
- 208000026350 Inborn Genetic disease Diseases 0.000 claims description 3
- 208000024556 Mendelian disease Diseases 0.000 claims description 3
- 210000003423 ankle Anatomy 0.000 claims description 3
- 230000007547 defect Effects 0.000 claims description 3
- 230000001850 reproductive effect Effects 0.000 claims description 3
- 238000010008 shearing Methods 0.000 claims description 3
- 241001416153 Bos grunniens Species 0.000 description 52
- 241001465754 Metazoa Species 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 244000144972 livestock Species 0.000 description 5
- 230000008569 process Effects 0.000 description 4
- 241000283690 Bos taurus Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 230000002550 fecal effect Effects 0.000 description 2
- 230000009027 insemination Effects 0.000 description 2
- 230000001734 parasympathetic effect Effects 0.000 description 2
- 230000033458 reproduction Effects 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 230000002889 sympathetic effect Effects 0.000 description 2
- 210000005239 tubule Anatomy 0.000 description 2
- 241000272517 Anseriformes Species 0.000 description 1
- 241001247317 Bos mutus Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 239000003708 ampul Substances 0.000 description 1
- 210000003403 autonomic nervous system Anatomy 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000012173 estrus Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000004377 microelectronic Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004118 muscle contraction Effects 0.000 description 1
- 210000001640 nerve ending Anatomy 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000037195 reproductive physiology Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000016160 smooth muscle contraction Effects 0.000 description 1
- 210000000278 spinal cord Anatomy 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
- 210000001177 vas deferen Anatomy 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
- A61D19/021—Apparatus for collecting seminal fluids; Artificial vaginas
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Reproductive Health (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Electrotherapy Devices (AREA)
Abstract
The invention relates to the technical field of semen collection, and discloses an electrical stimulation semen collection method for yaks, which comprises the following steps: preparing before semen collection: fixing the yaks in a roadway or a semen collection platform; cleaning the scrotum and the periphery of the scrotum of the male yak by using distilled water, and simultaneously cleaning the inside of the scrotum; electrical stimulation semen collection: switching on a power supply of the electrical stimulation semen collector, and regulating the voltage to 10V by a voltage regulating knob; inserting the stimulating electrode bar coated with vaseline or soap water into rectum of yak to an insertion depth of 40-45 cm; poking the inching switch to stimulate the sacral nerve of the male yak, simultaneously controlling the output voltage to enable the initial voltage to be 10V, the voltage rising amplitude to be 2V each time, the time for continuously outputting the voltage under each level of voltage to be 6-10s, the interval time to be 3-8s, circularly executing once, then rising the voltage by each level, and generally when the voltage rises to 24-30V, the semen of the yak can be collected. The method is simple to operate, and can efficiently collect the yak semen.
Description
Technical Field
The invention belongs to the technical field of semen collection, and particularly relates to an electric stimulation semen collection method for yaks.
Background
Artificial insemination is one of the important means of modern biotechnology, can exert excellent breeding value of male livestock to the maximum extent, improve the reproduction rate, and is widely applied to animal husbandry.
In the prior art, the common methods for animal semen collection mainly comprise a massage method and a false negative method, the two methods are commonly applied to semen collection of livestock such as cattle, sheep, pigs, chickens and the like, but the two semen collection methods are difficult to apply to most wild animals with strong wildness, violent temperament and small galleries and easy fright. With the development of the technology, people invent that the electrical stimulation method is applied to the semen collection work of male geese, and then the electrical stimulation method is widely applied to the artificial semen collection of other wild animals. Therefore, the electrical stimulation semen collection method has important significance for protecting and utilizing wild animal resources.
The problem of yak semen collection cannot be solved all the time due to the influences of factors such as strong wildness of wild yaks, hard living geographic environment and the like. In the prior art, after domestication of yaks in a Qinghai Datong breeding cattle farm in China, yak semen is successfully collected by adopting a pseudo-vaginal method and is prepared into tubule frozen semen. The artificial vagina method requires that yaks are well domesticated before artificial semen collection, and meanwhile, female yaks in estrus are matched, so that early work is very complicated. Therefore, an effective collection method for semen collection of wild yaks is needed.
Disclosure of Invention
The invention aims to provide an electric stimulation semen collection method for yaks, which is used for solving the technical problem that no method capable of effectively collecting semen of yaks exists in the prior art.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for electrically stimulating and semen collecting of yaks, which comprises the following steps:
preparing before semen collection:
selecting healthy, non-hereditary diseases, non-reproductive defects and adult yaks aged 4-8 years;
fixing the yak in a roadway or a semen collection platform, and fastening the head and the back ankle of the yak by using a rope;
shearing the abdominal hair of the male yaks by using a hair scissors, cleaning the scrotum and the periphery of the scrotum of the male yaks by using distilled water, simultaneously cleaning the inside of the scrotum, and then wiping water;
electrical stimulation semen collection:
switching on a power supply of the electrical stimulation semen collector, and regulating the voltage to 10V by a voltage regulating knob;
inserting the stimulating electrode bar coated with vaseline or soap water into rectum of yak to an insertion depth of 40-45 cm;
poking the inching switch to stimulate the sacral nerve of the male yak, simultaneously controlling the output voltage to enable the initial voltage to be 10V, the voltage rising amplitude to be 2V each time, the time for continuously outputting the voltage under each level of voltage to be 6-10s, the interval time to be 3-8s, circularly executing once, then rising the voltage by each level, and generally when the voltage rises to 24-30V, the semen of the yak can be collected.
Further, before a power switch of the electrical stimulation semen collector is turned on, a control switch of the electrical stimulation semen collector is turned off, the control mode is selected to be manual, and the voltage gear is selected to be 50V.
Further, the output voltage of the stimulating electrode is not higher than 46V.
Further, the interval time is 5 s.
Further, the semen collection frequency of the yaks does not exceed 2 times per week.
Further, the semen collection frequency of the yaks is 1 time per week.
Further, when the liquid discharged from the penis of the electrically stimulated male yak is not milky white, the non-milky white liquid is discarded until the liquid discharged from the penis is milky white, and semen collection is performed.
Further, when the electrode of the stimulation electrode bar inserted into the rectum of the yak feels that excrement is generated, the stimulation electrode bar is pulled out and the excrement is cleaned, and then the stimulation electrode bar is inserted into the rectum of the yak again to perform electric stimulation.
Has the advantages that: the yak electrical stimulation semen collection method only needs to do simple preparation work before semen collection, then operates and controls the electrical stimulation semen collection device to collect semen of the male yaks, and the process is simple, rapid and repeatable. For the male yaks with high seed value, especially the yaks with strong wildness and difficult access, effective semen collection can be realized by an electrical stimulation method without anaesthetizing the wild yaks. The obtained yak semen can be used for artificial insemination, semen cryopreservation, reproductive physiology research, descendant determination and the like, and has important significance for protecting and utilizing wild yak resources.
Detailed Description
The present invention will be further described with reference to preferred embodiments thereof. The examples are given solely for the purpose of illustration and are not intended to limit the scope of the invention.
Examples
First, it should be noted that the working principle of adopting the electrical stimulation method for semen collection in this embodiment is as follows: the autonomic nervous system innervates the male with an erection and ejaculation, which are innervated by parasympathetic and sympathetic nerves of the sacral spinal cord, respectively. The yak ejaculation can be divided into two processes: the first is the expulsion of semen into the urogenital tract, which is controlled by the sympathetic system, and the second is the ejection of semen out of the urogenital tract, which is parasympathetic. The electric stimulation semen collection of the yak is realized by directly stimulating the ejaculation center at the waist recommendation part by adopting an electrode. The electric stimulation semen collection method is based on physiological principles such as the reproductive physiological process and characteristics of the yak, combines the modern microelectronic technology, sets working mode and operating parameters by a pulse current generator, generates cooperative spectrum pulse under the control of a host CPU, transmits the cooperative spectrum pulse to an electrode (load) inserted into the rectum of the yak by a cable to generate an electric (current) field, and directly stimulates the nerve endings of a sperm line of the yak and the ampulla of the vas deferens by generating the pulse electric (current) field which stimulates the yak regularly from weak to strong to simulate the natural semen collection process, so that the nerve excitation is transmitted to the semen center of the yak to cause the excitation of the semen center of the yak, the muscle contraction, the penis erection and the smooth muscle contraction to collect the semen of the yak, and the aim of collecting the semen of the yak is achieved.
Next, it should be noted that the structure of the electrical stimulation semen collector adopted in this embodiment is: the electro-stimulation semen collector comprises a main machine (the main machine is formed by assembling a 12V direct-current battery, a pulse current generator, an automatic operation control button and a manual operation control table), pulse current output current, electrodes, a charging power supply, a handheld control button (namely a manual switch) and a semen collection funnel.
Specifically, this embodiment provides a yak electrical stimulation semen collection method, including:
preparing before semen collection:
selecting healthy, non-hereditary diseases, non-reproductive defects and adult yaks aged 4-8 years;
fixing the yak in a roadway or a semen collection platform, and fastening the head and the back ankle of the yak by using a rope;
it should be noted that the semen collection site selected in this embodiment is a site that is dry in terrain, wide, sanitary, and quiet in environment, and is close to a production laboratory. The electrical stimulation semen collection fixing frame can be designed and installed in a semen collection field according to the size and safety of the yak body, so that the semen collection bull is properly fixed, the safety of people and livestock is ensured, and semen collection is facilitated.
Shearing the abdominal hair of the male yaks by using a hair scissors, cleaning the scrotum and the periphery of the scrotum of the male yaks by using distilled water, simultaneously cleaning the inside of the scrotum, and then wiping water;
the method is characterized in that the subtraction of the abdominal hair of the yak has the following effects: the semen collection device is convenient for observing the discharged semen, collecting the semen and preventing the dust in the abdominal hair from polluting the semen.
Electrical stimulation semen collection:
switching on a power supply of the electrical stimulation semen collector, and regulating the voltage to 10V by a voltage regulating knob;
wherein, the process of switching on the power supply of the electrical stimulation semen collector comprises the following steps: one end of a power line is inserted into a socket of an instrument panel, the other end of the power line is connected with a 220V alternating current power supply, and a stimulation electrode bar is connected with two plugs and inserted into and output.
As a preferred embodiment, before the power switch of the electrical stimulation semen collector is turned on, the control switch of the electrical stimulation semen collector is turned off, the control mode is selected to be manual, and the voltage gear is selected to be 50V. Specifically, when the worker operates, the control switch is switched to the intermediate off state, and the control mode is selected as manual control.
Inserting the stimulating electrode bar coated with vaseline or soap water into rectum of yak to an insertion depth of 40-45 cm; preferably, the insertion depth can be adjusted according to the rectum of different yaks, and here, only one of the range intervals is provided, and the present embodiment is not limited thereto.
Poking the inching switch to stimulate the sacral nerve of the male yak, simultaneously controlling the output voltage to enable the initial voltage to be 10V, the voltage rising amplitude to be 2V each time, the time for continuously outputting the voltage under each level of voltage to be 6-10s, the interval time to be 3-8s, preferably 5s, circularly executing once, then rising the voltage by gear, and usually when the voltage rises to 24-30V, the semen of the yak can be collected.
When a worker toggles the electric switch to manually control the switch to keep the switch at the position of the jog switch, the duration time of the jog position is the time of continuously outputting voltage under each level of voltage, after fingers are loosened, the jog switch can automatically rebound to the middle closing position, at the moment, current cannot pass through the electrode bar, namely the interval time, and the yak is in the electrical stimulation intermission period.
Of course, it can be understood that the collection of the yak semen when the voltage is raised to 24-30V is only one embodiment of the present invention, and the interval is the output voltage required for collecting the yak semen under normal conditions. However, in practical application, due to the difference of the collected objects, some yaks may realize semen discharge at about 12V, and some yaks may need about 40V to discharge semen, and the yaks can be specifically adjusted according to the difference of the collected objects without limitation.
As an alternative embodiment, further, in order to avoid harming the body of the yak, the output voltage of the stimulation shocker should be limited within a range not higher than 46V, and the interval time should be limited within a range not lower than 3 s.
As an alternative embodiment, the sperm collection frequency of said yak is not more than 2 times per week. Preferably, the sperm collection frequency of the yaks is 1 time per week. The reason is that: after the same yak is used for semen collection twice a week, the volume and the quality of the semen of the second time are poorer than those of the semen of the first time, and meanwhile, the yak recovers the health and the speed of producing the semen is slower.
As an alternative embodiment, when the liquid discharged from the penis of the electrically stimulated male yak is non-opalescent, the non-opalescent liquid is discarded until the liquid discharged from the penis is opalescent, and semen collection is performed. Wherein the non-opalescent liquid is prostatic liquid, is generally transparent liquid, is mixed with a small amount of sperms, does not meet the requirement of sperm collection, and therefore, should be discarded.
As an alternative embodiment, when the electrode inserted into the rectum of the yak feels fecal, the stimulation electrode rod is pulled out and the fecal is cleaned, and then the stimulation electrode rod is inserted into the rectum of the yak again for electrical stimulation.
Application example
The method for collecting the semen of the yak is applied to a Longri livestock farm in Sichuan province, 30 times of semen collection is carried out on the yak in the livestock farm, and more than 2.5 million of tubule frozen semen of the yak is produced. The detailed semen collection and analysis records are as follows:
TABLE 12019 years yak semen collection record analysis table
TABLE 2-12020 years yak semen collection record analysis table
TABLE 2-22020 years yak semen collection record analysis table
TABLE 2-32020 years yak semen collection record analysis table
As can be seen from the tables 1 and 2-1 to 2-3, the yak electrical stimulation semen collection method successfully collects a plurality of yak semen with the semen volume of more than or equal to 5ml in 2019 and 2020, and the qualified probability of successfully freezing and storing each semen is up to more than 90% in 2020. Therefore, by adopting the yak electrical stimulation semen collection method, the yak semen can be efficiently obtained and frozen for storage for subsequent use.
Finally, it should be noted that: the above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. An electric stimulation semen collection method for yaks, which is characterized by comprising the following steps:
preparing before semen collection:
selecting healthy, non-hereditary diseases, non-reproductive defects and adult yaks aged 4-8 years;
fixing the yak in a roadway or a semen collection platform, and fastening the head and the back ankle of the yak by using a rope;
shearing the abdominal hair of the male yaks by using a hair scissors, cleaning the scrotum and the periphery of the scrotum of the male yaks by using distilled water, simultaneously cleaning the inside of the scrotum, and then wiping water;
electrical stimulation semen collection:
switching on a power supply of the electrical stimulation semen collector, and regulating the voltage to 10V by a voltage regulating knob;
inserting the stimulating electrode bar coated with vaseline or soap water into rectum of yak to an insertion depth of 40-45 cm;
poking the inching switch to stimulate the sacral nerve of the male yak, simultaneously controlling the output voltage to enable the initial voltage to be 10V, the voltage rising amplitude to be 2V each time, the time for continuously outputting the voltage under each level of voltage to be 6-10s, the interval time to be 3-8s, circularly executing once, then rising the voltage by each level, and generally when the voltage rises to 24-30V, the semen of the yak can be collected.
2. The yak electrical stimulation semen collection method according to claim 1, wherein before a power switch of the electrical stimulation semen collection device is turned on, a control switch of the electrical stimulation semen collection device is turned off, a control mode is selected to be manual, and a voltage gear is selected to be 50V.
3. The yak electrical stimulation semen collection method according to claim 2, wherein the output voltage of the stimulation electrode device is not higher than 46V.
4. The method for electrically stimulating and semen collecting of yaks as claimed in claim 3, wherein the time interval is 5 s.
5. The method for electrically stimulating and semen collection of yaks as claimed in claim 1, wherein the semen collection frequency of said male yaks is not more than 2 times per week.
6. The method for electrically stimulating and semen collection of yaks as claimed in claim 5, wherein the semen collection frequency of the male yaks is 1 time per week.
7. The method for electrically stimulating the yak to collect the semen according to claim 1, wherein when the liquid discharged from the penis of the electrically stimulated male yak is not milky white, the non-milky white liquid is discarded until the liquid discharged from the penis is milky white, and the semen collection is performed.
8. The yak electrical stimulation semen collection method according to claim 1, wherein when the electrode with the stimulation electrode bar inserted into the rectum of the yak feels that excrement is available, the stimulation electrode bar is pulled out and the excrement is cleaned, and then the stimulation electrode bar is inserted into the rectum of the yak again for electrical stimulation.
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| CN202110387621.XA CN113081377A (en) | 2021-04-09 | 2021-04-09 | Yak electrical stimulation semen collection method |
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| CN202110387621.XA CN113081377A (en) | 2021-04-09 | 2021-04-09 | Yak electrical stimulation semen collection method |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4564024A (en) * | 1984-07-02 | 1986-01-14 | Wohler Jr Wilson H | Electro-ejaculator probe |
| CN202714947U (en) * | 2012-07-09 | 2013-02-06 | 赵伟刚 | Automatic electrical stimulation semen collector |
| CN103126784A (en) * | 2013-03-06 | 2013-06-05 | 成都大熊猫繁育研究基地 | Rectum-massage electrical stimulating semen collecting method capable of improving semen collecting efficiency of pandas |
| CN104546218A (en) * | 2013-10-22 | 2015-04-29 | 云南省种畜繁育推广中心 | Electro-ejaculation method and application of oxen |
| CN107224339A (en) * | 2017-05-25 | 2017-10-03 | 中国热带农业科学院热带作物品种资源研究所 | One stud ram electro-ejaculation method |
| CN107822735A (en) * | 2017-11-02 | 2018-03-23 | 四川省龙日种畜场 | A kind of yak seminal fluid collecting method |
-
2021
- 2021-04-09 CN CN202110387621.XA patent/CN113081377A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4564024A (en) * | 1984-07-02 | 1986-01-14 | Wohler Jr Wilson H | Electro-ejaculator probe |
| CN202714947U (en) * | 2012-07-09 | 2013-02-06 | 赵伟刚 | Automatic electrical stimulation semen collector |
| CN103126784A (en) * | 2013-03-06 | 2013-06-05 | 成都大熊猫繁育研究基地 | Rectum-massage electrical stimulating semen collecting method capable of improving semen collecting efficiency of pandas |
| CN104546218A (en) * | 2013-10-22 | 2015-04-29 | 云南省种畜繁育推广中心 | Electro-ejaculation method and application of oxen |
| CN107224339A (en) * | 2017-05-25 | 2017-10-03 | 中国热带农业科学院热带作物品种资源研究所 | One stud ram electro-ejaculation method |
| CN107822735A (en) * | 2017-11-02 | 2018-03-23 | 四川省龙日种畜场 | A kind of yak seminal fluid collecting method |
Non-Patent Citations (2)
| Title |
|---|
| "《动物繁殖学》", 30 June 2013, 中国海洋大学出版社, pages: 180 - 181 * |
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