CN107207573A - 超敏aba受体 - Google Patents

超敏aba受体 Download PDF

Info

Publication number
CN107207573A
CN107207573A CN201580058071.XA CN201580058071A CN107207573A CN 107207573 A CN107207573 A CN 107207573A CN 201580058071 A CN201580058071 A CN 201580058071A CN 107207573 A CN107207573 A CN 107207573A
Authority
CN
China
Prior art keywords
arg
ser
val
glu
thr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201580058071.XA
Other languages
English (en)
Inventor
S·卡特勒
M·L·努西奥
Q·奎
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
Original Assignee
University of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California filed Critical University of California
Publication of CN107207573A publication Critical patent/CN107207573A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8291—Hormone-influenced development
    • C12N15/8293—Abscisic acid [ABA]

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

提供了超敏PYR/PYL多肽、组合物和方法。

Description

超敏ABA受体
相关申请的交叉参考
本专利申请要求2014年8月26日提交的美国临时专利申请号62/042,095和2014年12月30日提交的美国临时专利申请号62/098,025的优先权，上述文献通过引用纳入本文用于所有目的。
联邦资助的研究与开发下作出发明的权利声明
本发明是在国家科学基金会(National Science Foundation)授予的基金号1258175下由政府资助完成。政府享有本发明的一定权利。
发明背景
脱落酸(ABA)是一种植物激素，其调节与非生物胁迫响应相关联的信号转导，Cutler,S.R.等,Annu.Rev.Plant Biol.61,651–679(2010))。ABA信号转导途径已通过多种方法被开发以改善植物胁迫响应和相关的产量性状(Wang，Y.等Plant J.43，413–424(2005))。ABA对于植物的直接应用改善了它们的水利用效率(Rademacher,W.，Maisch,R.,Liessegang,J.和Jung,J.(1987)。作物植物的水消耗和产量形成受脱落酸的合成类似物的影响(Water consumption and yield formation in crop plants under the influenceof synthetic analogues of abscisic acid)。《用于农业和景观用途的植物生长调节物》(Plant growth regulators for agricultural and amenity use)。BCPC专题论文(BCPCMonograph),(36),53-66)；出于该原因，ABA激动剂的发现(Okamoto,M.等，Proc.Natl.Acad.Sci.U.S.A.110，12132–12137(2013)；Park,S.-Y.等.Science 324，1068–1071(2009))获得了越来越多的关注，因为该分子可能有利于提高作物产量。对于活化ABA途径的一个补充方法涉及通过遗传方法增加植物对于ABA的敏感性。例如，增加植物的ABA敏感性的法尼基转移酶β亚基基因的条件反义物改善了中度干旱下油菜和拟南芥的产量(Wang等，2005)。
近期发现，ABA通过结合至称作PYR/PYL蛋白的受体的可溶家族引发多种它的细胞响应。PYR/PYL蛋白属于称为START超家族的配体结合蛋白的大家族(Iyer,L.M.等，Proteins Struct.Funct.Bioinforma.43，134–144，2001；Ponting,C.P.和Aravind,L.(1999)。Trends Biochem.Sci.24,130–132 1999)。这些蛋白质包含保守的三维结构，该结构由七个反平行β片层组成，其环绕中央α螺旋，以形成"螺旋-柄"基序；这些结构元件一起形成用于结合ABA或其它激动剂的配体结合口袋。
结构和功能研究已发现一系列的构象变化和PYR/PYL受体与II型C蛋白磷酸酶(PP2C)之间的关键接触，这是通过受体的ABA介导的PP2C抑制所必需的。例如，当ABA或另一激动剂在PYR/PYL蛋白的配体结合口袋中结合时，其使构象变化稳定化，这允许该受体结合并抑制PP2C家族，其通常遏制ABA信号转导(Weiner等，2010)。具体而言，ABA结合导致侧接所述配体结合口袋的柔性"门(gate)"环的大型重排。ABA结合后，该门环采取封闭的构象，其通过该环与ABA之间的若干直接接触而被稳定化。该门的激动剂结合的封闭形式允许PYR/PYL蛋白停靠进入并抑制PP2C的活性位点。所得的抑制进而允许SnRK2类的下游激酶的活化，其对于ABA响应中涉及的转录因子、离子通道和其它蛋白质的活性的调节具有响应性(Weiner，J.J.等(2010)Curr.Opin.Plant Biol.13，495–5022010)。因此，该受体的封闭的门构象的稳定化在其活化中起一定作用，并且PYR/PYL受体是控制调节不同的ABA响应的信号转导级联的顶点的开关的分子。
除了门关闭在受体活化中所起的作用之外，也出现其它结构重排。例如，PYR1、PYL1和PYL2是溶液中的同源二聚体，但以单体形式结合至PP2C。该同源二聚体接口(interface)与PP2C结合接口重叠，因此完整受体同源二聚体无法结合并抑制PP2C。因此，二聚体形成对于ABA信号转导是拮抗的，并且受体二聚体-断裂是受体活化的必要步骤。此外，包含位于PP2C的中央保守色氨酸"锁(lock)"残基的识别模块插入在ABA-结合受体中形成的小孔。色氨酸锁残基的突变完全破坏受体介导的PP2C活性的失活，这证明了锁残基插入受体的孔中的作用。
野生型或突变ABA受体在转基因拟南芥(Arabidopsis thaliana)、番茄(Solanumlycopersicum)和水稻(Oryza sativa)中的过表达能改善干旱耐受(Gonzalez-Guzman，M.等(2014).J.Exp.Bot.eru219，2014；Kim等，J.Exp.Bot.63，1013–1024 2012；Santiago等，Plant J.60，575–588(2009))。相对于其野生型对应物具有增加的敏感性的ABA受体在植物中表达时能够引发更大的ABA响应。与此相一致的是，Pizzio等，Plant Physiol.163，441–455(2013)描述了PYL4突变A194T突变体，其相较于野生型PYL4需要较低浓度的ABA来引发测得到的ABA响应。当该突变体在转基因拟南芥中过表达时，该植物具有相对于野生型对照和PYL4过表达对照而言增加的对于ABA的敏感性(Pizzio等，Plant Physiol.163，441–455(2013))。并且，35S::PYL4A194T系显示优于野生型或35S::PYL4过表达系的干旱耐受和水利用。A194T突变位于PYL4的羧基末端，其是受体与受体之间在长度和组成方面高度可变的受体的部分。这一保守性的缺失导致难以预计突变改变ABA敏感性所利用的机制。
发明内容
已经鉴定导致PYR/PYL受体蛋白对于ABA是超敏(hypersensitive)的该受体蛋白中的突变。在一些实施方式中，提供编码所述蛋白质的核酸(例如，分离的核酸)。在一些实施方式中，所述核酸包含编码突变的PYR/PYL受体多肽的多核苷酸，所述突变的PYR/PYL受体多肽含有应于SEQ ID NO:1所示的PYR1中的氨基酸F61、V81、I110、E141和A160的氨基酸取代，其中，所述突变的PYR/PYL受体相较于缺乏所述取代的对照PYR/PYL受体对于脱落酸具有增加的敏感性。
在一些实施方式中，所述PYR/PYL受体多肽包含对应于氨基酸F61的氨基酸取代。在一些实施方式中，所述氨基酸取代选自L和M。
在一些实施方式中，所述PYR/PYL受体多肽包含对应于氨基酸V81的氨基酸取代。在一些实施方式中，所述氨基酸取代选自I和Y。
在一些实施方式中，所述PYR/PYL受体多肽包含对应于氨基酸I110的氨基酸取代。在一些实施方式中，所述氨基酸取代选自C和S。
在一些实施方式中，所述PYR/PYL受体多肽包含对应于氨基酸E141的氨基酸取代。在一些实施方式中，所述氨基酸取代选自C、I、L、M、N、T、V、W和Y。
在一些实施方式中，所述PYR/PYL受体多肽包含对应于氨基酸A160的氨基酸取代。在一些实施方式中，所述氨基酸取代选自C、I和V。
在一些实施方式中，所述PYR/PYL受体多肽包含对应于如下内容的氨基酸取代：
F61L和A160C；
F61M和A160V；
F61M、I110S和A160V；或
F61L、V81I、I110C和A160V。
在一些实施方式中，所述突变的PYR/PYL受体多肽基本相同于(例如，至少50％、55％、60％、65％、70％、75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％，或99％相同于)SEQ ID NO:1-119或SEQ ID NO:124-154中任一项(例如，124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153，或154)，155-361中任一项(例如，155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223、224、225、226、227、228、229、230、231、232、233、234、235、236、237、238、239、240、241、242、243、244、245、246、247、248、249、250、251、252、253、254、255、256、257、258、259、260、261、262、263、264、265、266、267、268、269、270、271、272、273、274、275、276、277、278、279、280、281、282、283、284、285、286、287、288、289、290、291、292、293、294、295、296、297、298、299、300、301、302、303、304、305、306、307、308、309、310、311、312、313、314、315、316、317、318、319、320、321、322、323、324、325、326、327、328、329、330、331、332、333、334、335、336、337、338、339、340、341、342、343、344、345、346、347、348、349、350、351、352、353、354、355、356、357、358、359、360，或361)或包含SEQ ID NO:120-123中任一项。
还提供植物(例如转基因或非转基因植物)，其含有如上文或本文他处所述的编码PYR/PYL受体多肽的多核苷酸，例如，含有对应于SEQ ID NO:1所示的PYR1中的氨基酸F61、V81、I110、E141和A160的氨基酸取代。在一些实施方式中，所述植物相较于缺乏所述多肽的对照植物具有增加的对于ABA的敏感性。在一些实施方式中，所述多核苷酸操作性地连接至异源性启动子。在一些实施方式中，所述多核苷酸操作性地连接至原始(非异源)启动子。在一些实施方式中，所述PYR/PYL受体多肽包含对应于如下内容的氨基酸取代：F61L和A160C；F61M和A160V；F61M、I110S和A160V；或F61L、V81I、I110C和A160V。在一些实施方式中，相较于所述植物的原始PYR/PYL受体多肽，编码的PYR/PYL受体多肽仅具有一个(或在一些实施方式中，仅2、3或4个)氨基酸取代。在一些实施方式中，所述植物的原始PYR/PYL受体多肽编码序列已被修饰(例如，通过CRISPR)以包含1、2、3或4个取代。在一些实施方式中，所述PYR/PYL受体多肽包含对应于氨基酸F61的氨基酸取代。在一些实施方式中，所述氨基酸取代选自L和M。在一些实施方式中，所述PYR/PYL受体多肽包含对应于氨基酸V81的氨基酸取代。在一些实施方式中，所述氨基酸取代选自I和Y。在一些实施方式中，所述PYR/PYL受体多肽包含对应于氨基酸I110的氨基酸取代。在一些实施方式中，所述氨基酸取代选自C和S。在一些实施方式中，所述PYR/PYL受体多肽包含对应于氨基酸E141的氨基酸取代。在一些实施方式中，所述氨基酸取代选自C、I、L、M、N、T、V、W和Y。在一些实施方式中，所述PYR/PYL受体多肽包含对应于氨基酸A160的氨基酸取代。在一些实施方式中，所述氨基酸取代选自C、I和V。在一些实施方式中，所述PYR/PYL受体多肽包含对应于如下内容的氨基酸取代：F61L和A160C；F61M和A160V；F61M、I110S和A160V；或F61L、V81I、I110C和A160V。
还提供植物(例如，包括但不限于玉米植物)，其含有原位突变的PYR/PYL受体多肽，该突变的PYR/PYL受体多肽含有对应于SEQ ID NO:1所示的PYR1中的氨基酸F61、V81、I110、E141和A160的氨基酸取代，其中，所述突变的PYR/PYL受体多肽相较于缺乏所述取代的对照PYR/PYL受体对于脱落酸具有增加的敏感性。在一些实施方式中，所述PYR/PYL受体多肽包含对应于氨基酸F61的氨基酸取代。在一些实施方式中，所述氨基酸取代选自L和M。在一些实施方式中，所述PYR/PYL受体多肽包含对应于氨基酸V81的氨基酸取代。在一些实施方式中，所述氨基酸取代选自I和Y。在一些实施方式中，所述PYR/PYL受体多肽包含对应于氨基酸I110的氨基酸取代。在一些实施方式中，所述氨基酸取代选自C和S。在一些实施方式中，所述PYR/PYL受体多肽包含对应于氨基酸E141的氨基酸取代。在一些实施方式中，所述氨基酸取代选自C、I、L、M、N、T、V、W和Y。在一些实施方式中，所述PYR/PYL受体多肽包含对应于氨基酸A160的氨基酸取代。在一些实施方式中，所述氨基酸取代选自C、I和V。在一些实施方式中，所述PYR/PYL受体多肽包含对应于如下内容的氨基酸取代：F61L和A160C；F61M和A160V；F61M、I110S和A160V；或F61L、V81I、I110C和A160V。在一些实施方式中，所述突变的PYR/PYL受体多肽基本相同于(例如，至少50％、55％、60％、65％、70％、75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％，或99％相同于)SEQ ID NO:1-119或SEQ ID NO:124-154中任一项(例如，124、125、126、127、128、129、130、131、132、133、134、135、136、137、138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153，或154)，155-361中任一项(例如，155、156、157、158、159、160、161、162、163、164、165、166、167、168、169、170、171、172、173、174、175、176、177、178、179、180、181、182、183、184、185、186、187、188、189、190、191、192、193、194、195、196、197、198、199、200、201、202、203、204、205、206、207、208、209、210、211、212、213、214、215、216、217、218、219、220、221、222、223、224、225、226、227、228、229、230、231、232、233、234、235、236、237、238、239、240、241、242、243、244、245、246、247、248、249、250、251、252、253、254、255、256、257、258、259、260、261、262、263、264、265、266、267、268、269、270、271、272、273、274、275、276、277、278、279、280、281、282、283、284、285、286、287、288、289、290、291、292、293、294、295、296、297、298、299、300、301、302、303、304、305、306、307、308、309、310、311、312、313、314、315、316、317、318、319、320、321、322、323、324、325、326、327、328、329、330、331、332、333、334、335、336、337、338、339、340、341、342、343、344、345、346、347、348、349、350、351、352、353、354、355、356、357、358、359、360，或361)或包含SEQ ID NO:120-123中任一项。
还提供包含启动子的表达盒，所述启动子操作性地连接至如上文或本文他处所述的编码PYR/PYL受体多肽的多核苷酸，例如，含有对应于SEQ ID NO:1所示的PYR1中的氨基酸F61、V81、I110、E141和A160的氨基酸取代，其中，将所述表达盒引入所述植物导致该植物相较于缺乏所述表达盒的对照植物对于脱落酸具有增加的敏感性。
在一些实施方式中，该启动子对于所述多核苷酸是异源的。在一些实施方式中，所述启动子是诱导型的。在一些实施方式中，所述启动子是胁迫诱导型启动子。
还提供表达载体，其含有如上文或本文他处所述的表达盒。
还提供植物，其含有如上文或本文他处所述的表达盒，其中，所述植物相较于缺乏所述表达盒的对照植物对于脱落酸具有增加的敏感性。还提供来自所述植物的植物细胞。
还提供来自本文所述的含有超敏PYR/PYL受体多肽的植物的种子、花、叶、果、经加工食物或食物成分。
还提供产生对于脱落酸具有增加的敏感性的植物的方法。在一些实施方式中，该方法包括：将编码本文所述的超敏PYR/PYL受体多肽的表达盒引入多个植物；和，从所述多个植物中选择表达所述多核苷酸的植物。
在一些实施方式中，该方法包括：将突变引入编码本文所述的超敏PYR/PYL多肽的多核苷酸，例如，其中，所述突变导致编码对应于SEQ ID NO:1所示的PYR1中的氨基酸F61、V81、I110、E141和A160的氨基酸取代的多核苷酸。在一些实施方式中，所述引入在植物细胞的基因组中原位发生。在一些实施方式中，所述引入包括规律成簇的间隔短回文重复(CRISPR)/Cas基因组编辑。
本文提供用于产生对于脱落酸具有增加的敏感性的植物(例如，玉米植物)的方法和试剂，所述方法包括将突变引入编码PYR/PYL多肽的多核苷酸，其中采用RNA引导的基因组修饰方法将所述突变原位引入所述植物的基因组。
在一个方面中，本文提供导向核糖核酸(gRNA)。在某些实施方式中，所述gRNA包括CRISPR核糖核酸(crRNA)，其基本相同于SEQ ID NO:363、364、365、366、367或369；和，反式作用核糖核酸(tracRNA)，其中，所述PYR/PYL突变靶位点包含编码PYL-E的V89或PYL-E的E149的核酸。
在所述gRNA的一些实施方式中，该PYR/PYL突变靶位点包括编码PYL-E的V89的核酸。在一些实施方式中，所述PYR/PYL突变靶位点具有SEQ ID NO:362的序列。
在一些实施方式中，所述PYR/PYL突变靶位点包括编码PYL-E的E149的核酸。在某些实施方式中，所述PYR/PYL突变靶位点具有SEQ ID NO:368的序列。
在某些实施方式中，所述tracRNA连接至gRNA的3’端。在具体实施方式中，所述tracRNA由具有基本相同于SEQ ID NO:370的序列的核苷酸编码。
在另一方面中，本文提供分离的核酸，其包括编码本文所述的任一gRNA的多核苷酸。
在另一方面中，本文提供表达盒，其包括RNA聚合酶启动子，该启动子操作性地连接至编码本文所述的任一gRNA的多核苷酸。在某些实施方式中，所述RNA聚合酶启动子是RNA聚合酶III(polIII)启动子。在具体实施方式中，所述polIII启动子是U3启动子或U6启动子。在一些实施方式中，所述表达盒具有SEQ ID NO:371-373中任一项的序列。
在另一方面中，本文提供表达载体，其包括表达盒，其中所述表达盒包括操作性地连接至编码本文所述的任一gRNA的多核苷酸的RNA聚合酶启动子。
在另一方面中，本文提供表达载体，其包括第一表达盒和第二表达盒。在某些实施方式中，所述第一表达盒是包括操作性地连接至编码本文所述的任一gRNA的多核苷酸的RNA聚合酶启动子的表达盒，并且，第二表达盒是含有操作性地连接至编码CRISPR相关联的内切核酸酶9(Cas9)的多核苷酸的启动子的表达盒。在一些实施方式中，所述表达载体包括第三表达盒，其中，所述第三表达盒是包括操作性地连接至编码本文所述的任一gRNA的多核苷酸的RNA聚合酶启动子的表达盒，并且所述第三表达盒不同于所述第一表达盒。
在一些实施方式中，所述表达载体包括第一、第二和第三表达盒，其中所述第一表达盒是包括操作性地连接至编码CRISPR相关联的内切核酸酶9(Cas9)的多核苷酸的启动子的表达盒，所述第二表达盒具有基本相同于SEQ ID NO:371或SEQ ID NO:372的序列，并且所述第三表达盒具有基本相同于SEQ ID NO:373的序列。在某些实施方式中，操作性地连接至编码Cas9的多核苷酸的启动子是泛素-1启动子(prUbi-10)。
在另一方面中，本文提供细胞，其包括上文所述或本文他处所述的任何表达载体。
还提供植物，其包括如上文或本文他处所述的表达载体。在一些实施方式中，所述植物是玉米植物。
还提供来自上文所述或本文他处的植物的植物细胞。
在另一方面中，本文提供来自上文所述或本文他处的植物的种子、花、叶、果、经加工食物或食物成分。在某些实施方式中，将所述表达载体引入上文所述或本文他处所述的植物导致该植物相较于缺乏所述表达盒的对照植物对于脱落酸具有增加的敏感性。
在另一方面中，本文提供产生在基因组PYR/PYL突变靶位点处具有突变的植物的方法。在一些实施方式中，所述方法包括将表达载体引入植物细胞，该表达载体包括编码gRNA和Cas9的多核苷酸，如上文或本文他处所述，和，含有所述突变的至少一种修复核酸(repair nucleic acid)。在某些实施方式中，通过在基因组PYR/PYLR突变靶位点中的Cas9切割事件之后同源重组将所述突变引入基因组PYR/PYLR突变靶位点。在一些实施方式中，所述方法还包括选择具有所述突变的植物细胞；由此产生所述植物。在一些实施方式中，所述引入在植物细胞的基因组中原位发生。在一些实施方式中，所述突变通过将所述表达载体和至少一种修复核酸引入植物胚细胞来引入，其中，所述植物胚的基因组包含PYR/PYL突变靶位点，并且其中，所述修复核酸包含所述突变，并通过在PYL-E突变靶位点的Cas9切割事件之后的同源重组将所述突变在PYR/PYL突变靶位点处引入。
在一些实施方式中，所述修复核酸具有基本相同于SEQ ID NO:375-387任一项所示序列的序列。在某些实施方式中，所述修复核酸具有基本相同于SEQ ID NO:377的序列。在一些实施方式中，所述修复核酸具有基本相同于SEQ ID NO:387的序列的序列。在其它实施方式中，引入两种修复核酸，并且其中，所述修复核酸具有基本相同于SEQ ID NO:377和SEQ ID NO:379的序列。在具体实施方式中，所述植物是玉米植物。
在另一方面中，本文提供试剂盒，其包括表达载体，所述表达载体包括编码gRNA的多核苷酸和编码Cas9的多核苷酸，如上文或本文他处所述，和至少一种修复核酸，其中，所述修复核酸包含PYL-E突变，并且能够通过Cas9切割事件之后的同源重组将PYL-E突变原位引入植物细胞基因组。在一些实施方式中，所述至少一种修复核酸具有基本相同于SEQ IDNO::374-386的序列。
在另一方面中，本文提供分离的核酸，其含有编码含有对应于PYL-E中的氨基酸V89的氨基酸取代的突变的PYR/PYL受体多肽的多核苷酸，其中，所述氨基酸取代是A(SEQID NO:389)。在一些实施方式中，所述突变的PYR/PYL受体多肽还包含对应于氨基酸E149的氨基酸取代。在某些实施方式中，所述对应于氨基酸E149的氨基酸取代是L(SEQ ID NO:390)。
而在另一方面中，本文提供分离的核酸，其含有编码包含突变的PYR/PYL受体多肽和融合伴侣多肽的融合蛋白的多核苷酸，其中，所述突变的PYR/PYL受体多肽包含对应于PYL-E中的氨基酸V89的氨基酸取代，其中，所述氨基酸取代是A。在某些实施方式中，所述突变的PYR/PYL受体多肽还包含对应于氨基酸E149的氨基酸取代。在具体实施方式中，所述对应于氨基酸E149的氨基酸取代是L。
在一些实施方式中，所述融合伴侣多肽包括转录活化结构域或转录调节结构域。在某些实施方式中，所述转录活化结构域是VP16或VP64。在某些实施方式中，所述融合蛋白还包含核定位信号序列。在一些实施方式中，所述突变的PYR/PYL受体多肽相较于缺乏所述取代的对照PYR/PYL受体多肽对于脱落酸具有增加的敏感性。
本文提供含有如上文或本文他处所述的多核苷酸的细胞。在某些实施方式中，所述多核苷酸是异源性多肽。在一些实施方式中，所述细胞是非植物真核细胞。
而在另一实施方式中，本文提供植物，其含如上文或本文他处所述的多核苷酸。在某些实施方式中，所述植物是玉米植物。
在另一个实施方式中，本文提供表达盒，其含有操作性地连接至如上文或本文他处所述的多核苷酸的启动子。在一些实施方式中，该启动子对于所述多核苷酸是异源的。在某些实施方式中，所述启动子是诱导型的。在一些实施方式中，所述启动子是胁迫诱导型启动子。
在另一个实施方式中，本文提供含有如上文或本文他处所述的表达盒的表达载体。
在另一方面中，本文提供植物，其包括如上文或本文他处所述的表达盒。在另一方面中，本文提供来自如上文或本文他处所述的植物的植物细胞。而在另一方面中，本文提供来自如上文或本文他处所述的植物的种子、花、叶、果、经加工食物或食物成分。
本文他处描述本发明的其他方面。
定义
术语"PYR/PYL受体多肽"指这样的蛋白质，其部分特点为存在聚酮环化酶结构域2(PF10604)、聚酮环化酶结构域1(PF03364)和Bet V I结构域(PF03364)中的一个或多个或全部，它们野生型形式中介导脱落酸(ABA)和ABA类似物信号转导。本领域已知多种PYR/PYL受体多肽序列。在一些实施方式中，PYR/PYL受体多肽包含基本相同于PYR1(SEQ ID NO:1)、PYL1(SEQ ID NO:2)、PYL2(SEQ ID NO:3)、PYL3(SEQ ID NO:4)、PYL4(SEQ ID NO:5)、PYL5(SEQ ID NO:6)、PYL6(SEQ ID NO:7)、PYL7(SEQ ID NO:8)、PYL8(SEQ ID NO:9)、PYL9(SEQID NO:10)、PYL10(SEQ ID NO:11)、PYL11(SEQ ID NO:12)、PYL12(SEQ ID NO:13)，或PYL13(SEQ ID NO:14)或SEQ ID NO:15-119中任一项的多肽。
"野生型PYR/PYL受体多肽"指天然产生的PYR/PYL受体多肽，其介导脱落酸(ABA)和ABA类似物信号转导。
"突变的PYR/PYL受体多肽"指这样的PYR/PYL受体多肽：其是来自天然产生的(即，野生型)PYR/PYL受体多肽的变体。本文所用的，突变的PYR/PYL受体多肽，相对于对应野生型PYR/PYL受体多肽包含一个、两个、三个、四个或更多个氨基酸取代，同时保留该受体的ABA-响应性。在该内容中，"突变的"多肽可通过用于产生非野生型核苷酸序列的任何方法产生。在一些实施方式中，突变的PYR/PYL受体多肽是高敏的，这表示该突变受体多肽通过ABA的活化强于通过相同浓度的ABA活化的对应同源野生型受体(或至少相较于在本文所述的突变位置处具有野生型氨基酸的其它情况相同的PYR/PYL多肽)，或者，相比对应同源野生型受体的活化，所述突变受体多肽通过较低(例如，低一半或更低)浓度的ABA活化，或同时满足两者。在一些实施方式中，所述突变受体多肽可在HAB1酵母双杂交试验通过视检确定响应0.25μM或更少的ABA。
"氨基酸取代"指，用与天然产生的残基不同的氨基酸残基替代给定位置处的天然产生的氨基酸残基(例如，野生型PYR/PYL受体多肽中存在的天然产生的氨基酸残基)。例如，野生型PYR1受体多肽序列(SEQ ID NO:1)的位置60处的天然产生的氨基酸残基是组氨酸(H60)；因此，H60处的氨基酸取代指用非组氨酸的任何氨基酸残基替代该天然产生的组氨酸。
"对应于[特定序列]中的氨基酸残基[X]"的氨基酸残基，或"对应于[特定序列]中的氨基酸取代[X]"的氨基酸取代指，与特定序列的等同的氨基酸比对(align)的感兴趣的多肽中的氨基酸。一般而言，本文所述的对应于特定PYR/PYL受体多肽序列的位置的氨基酸可以采用比对算法例如BLAST来确定。在本发明的一些实施方式中，氨基酸位置的"对应性"通过比对至含有SEQ ID NO:1的PYR/PYL受体多肽的区域来确定，如本文进一步详细讨论。当PYR/PYL受体多肽序列与SEQ ID NO:1不同时(例如，氨基酸变化或氨基酸添加或缺失)，与PYR/PYL受体的超敏活性相关联的具体的突变可能不位于其在SEQ ID NO:1中的相同位置编号中。例如，PYL2(SEQ ID NO:3)的氨基酸位置V85比对PYR1(SEQ ID NO:1)的氨基酸位置V81，这可以在两个序列的比对中容易地说明。在该示例中，SEQ ID NO:3中的氨基酸位置85对应于SEQ ID NO:1中的位置81。对应位置的示例示于图2。
当如下述比对最大对应性时，如果在两条序列中核苷酸或氨基酸残基的序列分别是相同的，两条核酸序列或多肽被称为“相同的”。在两条或更多条核酸或多肽序列内容中的术语“相同的”或“相同性”百分比指就比较窗的最大对应性进行比较和比对时，相同或有特定百分数的相同氨基酸残基或核苷酸的两条或更多条序列或子序列，如使用下列序列比较算法之一或通过手工比对和目测测量。序列相同性百分比涉及蛋白和肽使用时，认为不同的残基位置通常由于保守氨基酸取代而不同，其中氨基酸残基被具有相似化学性质(如电荷或疏水性)的其他氨基酸残基取代，因此不改变分子的功能性质。当序列由于保守取代而不同时，序列相同性百分比可上调以根据该取代的保守性质校正。进行该调整的方法为本领域技术人员公知。通常其包括将保守取代作为部分评分而不是完全错配，从而增加序列相同性百分比。因此例如，相同氨基酸得分为1，非保守取代得分为0，保守取代得分为0-1。保守取代的评分按照，例如Meyers和Miller的算法，Computer Applic.Biol.Sci.4:11-11(1988)，例如，如在程序PC/基因(美国加利福尼亚州芒廷维尤的智慧遗传公司(Intelligenetics，Mountain View，California，USA))中执行的进行计算。
在两种核酸或多肽的内容中使用的短语“基本相同”或“基本等同”是指一条序列与参比序列相比具有至少50％的序列相同性。或者，百分比相同性可以是50％-100％之间的任意整数。在一些实施方式中，如果采用本文所述的方法(优选是采用标准参数的BLAST，如下所述)确定序列与参比序列具有至少50％、55％、60％、65％、70％、75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％，或99％的序列相同性，则该序列基本相同于参比序列。本发明的实施方式提供编码基本相同于SEQ ID NO:1-119或SEQ ID NO:155-361中任一项的多肽的核酸。
对于序列比较，一般将一条序列用作与测试序列比较的参比序列。使用序列比较算法时，测试和参比序列均输入计算机，必要时指定子序列坐标，指定序列算法程序参数。可使用默认的程序参数，或者可指定另外的参数。然后，该序列比较算法基于程序参数计算出测试序列相对于参比序列的序列相同性百分数。
本文所用的“比较窗”包括参考选自20-600，通常约50-200，更常见约100-150数量的毗连位置的区段，对两个序列进行最优比对后，可将该区段的序列与相同数量的毗连位置的参比序列作比较。比对序列的比较方法是本领域熟知的。可进行最优序列比对以作比较，例如，通过Smith和水man的局部同源性算法，Adv.Appl.Math.2:482(1981)；通过Needleman和Wunsch的同源性比对算法，J.Mol.Biol.48:443(1970)；通过Pearson和Lipman的相似性搜索法，Proc.Nat’l.Acad.Sci.USA 85:2444(1988)；通过计算机执行这些算法(遗传学计算组(Genetics Computer Group)的威斯康星遗传学软件包(WisconsinGenetics软件Package)中的GAP、BESTFIT、FASTA和TFASTA，575科学大道(Science Dr.)，威斯康星州麦迪逊(Madison，WI))，或通过手工比对和目测。
适合测定序列相同性百分数和序列相似性百分数的算法是BLAST和BLAST 2.0算法，分别描述于Altschul等(1990)J.Mol.Biol.215:403-410和Altschul等(1977)NucleicAcids Res.25:3389-3402。进行BLAST分析的软件可通过国家生物技术信息中心(NationalCenter for BiotechnologyIn形成)(NCBI)网站从公共渠道获得。此算法包括：首先通过鉴定查询序列中长度为W的短字来鉴定高评分序列对(HSP)，与数据库序列中长度相同的字比对时它们能匹配或满足一些正值的阈值评分T。T称为相邻字评分阈值(Altschul等，同上)。这些初始相邻字命中用作启动搜索的种子，以便找到含有它们的较长HSP。然后，沿各个序列在两个方向上延伸该字命中，直到提高累积的比对评分。就核苷酸序列而言，采用参数M(一对匹配残基的奖励评分；总是>0)和N(错配残基的罚分；总是<0)计算累积评分。就氨基酸序列而言，用评分矩阵计算累积评分。出现以下情况时中止字命中在各个方向上的延伸：累积比对评分比其最大获得值降低X；由于一个或多个负评分残基比对的累积，累积评分变为零或零以下；或者达到任一序列的末端。BLAST算法参数W、T和X决定比对的灵敏度和速度。BLASTN程序(用于核苷酸序列)采用的默认值如下：字长(W)28，期望值(E)10，M＝1，N＝-2，以及比较两条链。就氨基酸序列而言，BLASTP程序使用默认参数，字长(W)3、期望值(E)10、和BLOSUM62评分矩阵(参见Henikoff和Henikoff(1989)Proc Natl Acad Sci USA 89:10915(1989))。
BLAST算法也对两条序列间的相似性进行统计学分析(参见例如，Karlin和Altschul，Proc.Nat’l.Acad.Sci.USA 90:5873-5787(1993))。BLAST算法提供的一种相似性测量是最小概率和(P(N))，它表明两条核苷酸或氨基酸序列之间偶尔发生匹配的概率。例如，如果测试核酸与参比核酸的比较中最小概率和小于约0.01，更优选小于约10-5，最优选小于约10-20，则认为该核酸与参比序列相似。
本文所用的术语“启动子”是指在细胞中能够驱动编码序列转录的多核苷酸序列。因此，在本发明的多核苷酸构建体中使用的启动子包括参与调节或调整基因转录的周期和/或速率的顺式作用的转录控制元件和调节序列。例如，启动子可以是包括参与转录调节的增强子、启动子、转录终止子、复制起点、染色体整合序列、5'和3'未翻译区域或内含子序列的顺式作用转录控制元件。顺式作用序列通常与蛋白或其它生物分子相互作用来进行(打开/关闭、调节、调整等)基因转录。"植物启动子"是能够起始植物细胞中的转录的启动子。"组成型启动子"是能够起始几乎全部组织类型中的转录的启动子，而"组织特异性启动子"仅起始一种或少数几种具体的组织类型中的转录。
如果来源于外来物种，或者来源于相同物种但是从其原始形式修饰，则多核苷酸序列对于生物体或次生多核苷酸序列来说是“异源的”。例如，当启动子与异源编码序列所谓操作性地连接时，其表示该编码序列衍生自一个物种而该启动子序列衍生自另一个不同的物种；或者，如果两者都衍生自相同物种，则该编码序列并不与该启动子天然相关(例如，是遗传工程改造的编码序列，例如，来自相同物种中的不同基因，或来自不同生态型或变种的等位基因)。
“表达盒”是指当导入宿主细胞时，分别导致RNA或多肽的转录和/或翻译的核酸构建体。没有或不能被翻译的反义和正义构建体明确地包含在该定义中。在转基因的表达和内源性基因的抑制的情况下(例如，通过反义或正义抑制)，本领域技术人员会意识到插入的多核苷酸序列不需要是相同的，但可以是仅仅与其衍生的基因的序列基本相同的。如本文解释，这些基本相同的变体由特定核酸序列的参考特定覆盖。
术语"宿主细胞"指能够复制和/或转录和/或翻译异源性多核苷酸的任何细胞。因此，"宿主细胞"指任何原核细胞(包括但不限于大肠杆菌)或真核细胞(包括但不限于酵母细胞、哺乳动物细胞、禽细胞、两栖动物细胞、植物细胞、鱼细胞和昆虫细胞)，无论位于体外还是体内。例如，宿主细胞可位于转基因动物或转基因植物中。原核细胞(包括但不限于大肠杆菌)或真核细胞(包括但不限于酵母细胞、哺乳动物细胞、禽细胞、两栖动物细胞、植物细胞、鱼细胞和昆虫细胞)。宿主细胞可以例如用该异源性多核苷酸转化。
术语"植物"包括完整植物、芽滋养型器官和/或结构(例如，叶、茎和块茎)、根、花和花器官(例如，苞片、萼片、花瓣、雄蕊、心皮、花药)，胚珠(包括卵和中央细胞)、种子(包括受精卵、胚、胚乳，和种皮)，果(例如，成熟卵巢)，幼苗，植物组织(例如，维管组织、基本组织等)，细胞(例如，保卫细胞、卵细胞、毛状体等)，及其后裔。本发明所述方法可用的植物种类通常宽泛到可使用转化技术的高等和低等植物，包括被子植物(单子叶和双子叶植物)、裸子植物、蕨类和多细胞藻类。其包括各种倍性水平的植物，包括非整倍体、多倍体、二倍体、单倍体和半合子。
附图的简要说明
图1提供酵母双杂交试验中的信号，其中ABA浓度显示在上部，并且突变体相同性显示在左侧。
图2提供对于多种不同PYR/PYL蛋白，在本文所述的五个位置处的对应天然产生的氨基酸。
图3提供多种PYR/PYL蛋白的中间部分的比对。(SEQ ID NO:142-154)
图4提供酵母双杂交试验中的信号，并且包括PYR1中的多重突变。
图5描述生物弹射转化载体pZmPYLE-V89A，其携带用于玉米优化的Cas9和ZmPYLE-V89A gRNA的表达盒，以介导位于ZmPYL-E靶序列(5’-CGCGA CGTCA ACGTC AAGAC-3’)的切割。
图6提供二元载体pZmPYLE-E149L的示意图，其用于采用土壤杆菌介导的转化的递送。
图7提供质粒载体pZmPYLE-V89A-E149L的示意图，其携带用于2种不同gRNA和Cas9的表达盒。
图8提供二元植物转化载体23190的示意图，其携带用于介导ZmPYL-E E149L诱变的Cas9、gRNA和选择性标志物基因PMI的表达盒。
图9A-B提供二元植物转化载体23136和23189的示意图，其携带用于介导ZmPYL-DE169L诱变的Cas9、gRNA和选择性标志物基因PMI的表达盒。
图10A-10B提供二元植物植物转化载体22981和23191的示意图，其携带介导ZmPYL-F E164L诱变的Cas9、gRNA和选择性标志物基因PMI的表达盒。
图11提供二元植物植物转化载体23192的示意图，其携带用于介导ZmPYL-E E148L的Cas9、gRNA和选择性标志物基因PMI的表达盒。
图12提供终点试验实施例的示意图，以检测导致E164L氨基酸残基突变的ZmPYL-F中的特定DNA序列变化(GA至CT)。
发明详述
I.绪论
为了鉴定造成增加的受体ABA敏感性的突变，我们采用PYR1突变体集合，其包含位于ABA紧密附近的所有可能的单一氨基酸取代残基，筛选了降低诱导可检测的PYR1和HAB1相互作用所需的ABA浓度的突变体。基于这些结果，我们描述了显著增加受体ABA敏感性的高度保守残基中的突变。
已发现导致超敏形式的PYR/PYL受体的PYR/PYL受体多肽中的突变，即，突变的受体对于ABA的响应性高于对应野生型PYR/PYL多肽。
本文所述的一种或多种超敏突变PYR/PYl受体多肽在植物中的表达将导致植物具有增加的ABA-敏感性，且在一些实施方式中，导致更高的胁迫耐受或与ABA-响应性相关的其它表型。
本文还提供用于产生对于脱落酸具有增加的敏感性的植物(例如，玉米植物)的方法和试剂，所述方法包括将突变引入编码PYR/PYL多肽的多核苷酸，其中采用RNA引导的基因组修饰方法将所述突变原位引入所述植物的基因组。
II.超敏PYR/PYL受体多肽
多种野生型(天然产生的)PYR/PYL多肽序列是本领域已知的。尽管PYR1起初在拟南芥中被鉴定为脱落酸(ABA)受体，实际上，PYR1是在拟南芥中属于相同蛋白质家族的至少14蛋白质(PYR/PYL蛋白)的组的成员，其还介导ABA信号转导。该蛋白质家族也存在于其它植物中(参见例如，序列表)，并且部分表征为存在聚酮环化酶结构域2(PF10604)、聚酮环化酶结构域1(PF03364)和Bet V I结构域(PF03364)中的一个或多个或全部。START/Bet v 1超家族结构域描述于，例如，Radauer，BMC Evol.Biol.8:286(2008)。在一些实施方式中，野生型PYR/PYL受体多肽包含SEQ ID NO:1-119中的任一项。在一些实施方式中，野生型PYR/PYL受体多肽基本相同于(例如，至少50％、55％、60％、65％、70％、75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％，或99％相同于)SEQ ID NO:1-119中的任一项。
PYR/PYL受体蛋白具有保守的START-结构域配体结合口袋，其通过称作"门"和"闩"的两个环侧接(Melcher,K.等，Nature 462(2009))。ABA在配体结合口袋处结合至PYR/PYL受体蛋白，并且，ABA结合诱导环的关闭，以将ABA封闭在配体结合口袋内。PYR/PYL受体多肽的配体结合口袋包含与PYR/PYL配体(例如，ABA)或该配体与PYR/PYL受体结合时的配体-接触水分子紧密靠近(例如，约内)的氨基酸残基。由25个残基组成该PYR1配体结合口袋。该配体结合口袋的残基在其它PYR/PYL家族成员中也是高度保守的。
PYR/PYL受体蛋白直接结合至2型蛋白磷酸酶(PP2C)，因此还包含PP2C结合接口。在PP2C、PYR/PYL受体和ABA以三元复合物的形式结合在一起时，PYR/PYL受体多肽的PP2C结合接口包含与PP2C紧密靠近(例如，约以内)的氨基酸残基。由25个残基组成PYR1PP2C结合接口。PP2C结合接口的残基在其它PYR/PYL家族成员中也是高度保守的。
超敏PYR/PYL受体多肽是来自天然产生的(即，野生型)PYR/PYL受体多肽的非天然产生的变体，其中，所述变体(突变体)PYR/PYL受体多肽能够在脱落酸的存在下以高于相同浓度的ABA的存在下的对照PYR/PYL受体多肽的程度结合至和/或抑制PP2C的活性。相较于野生型PYR/PYL受体多肽，本文所述的超敏活性PYR/PYL受体多肽包含一个或多个氨基酸取代。在一些实施方式中，超敏PYR/PYL受体多肽基本相同于(例如，至少50％、55％、60％、65％、70％、75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％，或99％相同于)SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118，或119中任一项，并且包含本文所述的1、2、3、4或更多个突变(例如，氨基酸取代)。在一些实施方式中，超敏PYR/PYL受体多肽包含SEQ ID NO:120、121、122或123且包含本文所述的1、2、3、4或更多个突变(例如，氨基酸取代)：
CxSxxxxxxxAPxxxxWxxxxxFxxPxxxxxFxxxC(SEQ ID NO:120)
GxxRxVxxxSxxPAxxSxExLxxxD(SEQ ID NO:121)
GGxHRLxNYxS(SEQ ID NO:122)
ESxxVDxPxGxxxxxTxxFxxxxxxxNLxxL(SEQ ID NO:123)。
如实施例中所示，已发现可在PYR/PYL受体多肽的数个位置中的任意处进行突变以导致对于ABA的超敏性。这些位置是(对应于其在拟南芥PYR1(SEQ ID NO:1)中的位置)：F61、V81、I110、E141和A160。在一些实施方式中，突变的PYR/PYL受体多肽包含对应于这些位置的一个或多个(例如，一个、两个、三个或四个)氨基酸取代。例如，在一些实施方式中，所述突变的PYR/PYL受体多肽包含至少如下对应突变：
F61L和A160C；
F61M和A160V；
F61M、I110S和A160V；或
F61L、V81I、I110C和A160V。
SEQ ID NO:1；拟南芥野生型PYR1
对于位置F61(对应于SEQ ID NO:1中的位置)，超敏突变将包括F61L或F61M。对于位置V81(对应于SEQ ID NO:1中的位置)，超敏突变将包括V81I或V81Y。对于位置I110(对应于SEQ ID NO:1中的位置)，超敏突变将包括I110C或I110S。由于一些原始PYR/PYL多肽在对应于SEQ ID NO:1的I110的位置处具有缬氨酸，在其中位置I110突变的一些实施方式中，原始氨基酸将是缬氨酸，后续突变成C或S。对于位置E141(对应于SEQ ID NO:1中的位置)，超敏突变将包括E141C、E141I、E141L、E141M、E141N、E141T、E141V、E141W，或E141Y。对于位置A160(对应于SEQ ID NO:1中的位置)，超敏突变将包括A160C、A160I或A160V。由于一些原始PYR/PYL多肽在对应于SEQ ID NO:1的A160位置处具有缬氨酸，在其中位置A160突变的一些实施方式中，原始氨基酸将是缬氨酸，后续突变成C或I。
本文所述的任何突变可在任何野生型PYR/PYL多肽，例如，在SEQ ID NO:1-119的任何多肽或基本相同于SEQ ID NO:1-119中任一项或含有SEQ ID NO:120-123中任一项的多肽中进行。通过比对将待突变的PYR/PYL受体多肽序列与如SEQ ID NO:1所示的PYR1受体多肽序列相比对，类似的氨基酸取代可在，例如，不同于PYR1的PYR/PYL受体中进行。作为非限制性示例，类似于SEQ ID NO:1所示的PYR1中的氨基酸取代V81I的PYL2中的氨基酸取代可通过如下方式确定：将PYL2(SEQ ID NO:3)和PYR1(SEQ ID NO:1)的氨基酸序列比对并鉴定与PYR1(SEQ ID NO:1)的氨基酸位置V81比对的PYL2中的位置V85。PYR/PYL受体中类似的氨基酸位置示于图2和3。例如，SEQ ID NO:155-361代表包含本文所述的超敏突变的玉米PYR/PYL多肽。应理解，所述多肽可进一步突变(例如，具有保守突变，例如，在活性位点之外)而基本不影响活性。因此，在一些实施方式中，本文所述的超敏多肽包含基本(例如，至少70％、75％、80％、85％、90％、95％、98％)相同于SEQ ID NO:155-361之一的完整序列的序列。
PYR/PYL受体活性中一个或多个氨基酸取代使该受体对ABA超敏的程度可定量检测，例如，通过检测ABA和含有一个或多个氨基酸取代的PYR/PYL受体的存在下的磷酸酶活性，并将该磷酸酶活性与对照PYR/PYL受体做比较。对照PYR/PYL受体通常是与该突变的PYR/PYL多肽最相似的野生型PYR/PYL多肽。在一些实施方式中，例如，当起始蛋白质不是野生型PYR/PYL多肽时，对照PYR/PYL多肽可基本相同(例如，至少90、95或98％相同)于测试PYR/PYL多肽(即，疑似高敏的)且在对应位置具有野生型氨基酸。例如，如果突变PYR/PYL受体具有F61X突变，其中X是任何非F氨基酸，则对照将在相同位置具有F61但将在其它位置相同于该突变PYR/PYL受体。如果突变PYR/PYL受体具有V81X突变，其中X是任何非V氨基酸，则对照将在相同位置具有V81但将在其它位置相同于该突变PYR/PYL受体。如果突变PYR/PYL受体具有I110X突变，其中X是任何非I、非V氨基酸，则对照将在相同位置具有I110或V但将在其它位置相同于该突变PYR/PYL受体。如果突变PYR/PYL受体具有E141X突变，其中X是任何非E氨基酸，则对照将在相同位置具有E141但将在其它位置相同于该突变PYR/PYL受体。如果突变PYR/PYL受体具有A160X突变，其中X是任何非A、非V氨基酸，则对照将在相同位置具有A160或缬氨酸但将在其它位置相同于该突变PYR/PYL受体。
在一些实施方式中，突变的PYR/PYL受体多肽包含本文所述的两个或更多个氨基酸取代。在一些实施方式中，所述两个或更多个氨基酸取代对应于，SEQ ID NO:1所示的PYR1中的F61X、V81X、I110X、E141X和A160X，其中X是本文中所示导致超敏性的氨基酸。
提供本发明的实施方式用于上述蛋白质和/或核酸序列、编码所述多肽，在本发明方法和组合物中(例如，具有原位PYR/PYL修饰的植物、转基因植物、表达盒等)。编码植物野生型PYR/PYL受体的多核苷酸序列的分离(例如，从其中PYR/PYL序列尚未经鉴定的植物)可通过多种技术完成。例如，基于本文公开的PYR/PYL编码序列(例如，列于序列表中)的寡核苷酸探针可用以在cDNA或基因组DNA文库中鉴定所需野生型PYR/PYL基因。为了构建基因组文库，通过随机片段化，例如，采用限制性内切核酸酶，产生基因组DNA的大区段，然后将其与载体DNA连接以形成多联体，其可被包装进入合适的载体。为了制备cDNA文库，mRNA从所需组织(例如具体植物物种的叶)分离，然后由所述mRNA制备包含感兴趣的基因转录本的cDNA文库。或者，cDNA可从由其中表达PYR/PYL基因的其它组织提取的mRNA制备。
然后，cDNA或基因组文库可基于本文所述的PYR/PYL基因的序列，采用探针筛选。可采用探针来与基因组DNA或cDNA序列杂交，以分离相同或不同植物物种中的同源基因。或者，可采用针对多肽产生的抗体以筛选mRNA表达文库。
或者，编码PYR/PYL的核酸可采用扩增技术从核酸样品扩增。例如，可采用聚合酶链式反应(PCR)技术来直接从基因组DNA，从cDNA，从基因组文库或cDNA文库扩增PYR/PYL的编码序列。PCR和其它体外扩增方法也可以是有用的，例如，克隆编码待表达的PYR/PYL的多核苷酸序列，以使核酸用作探针用于检测样品中所需mRNA的存在，以供核酸测序，或用于其它目的。PCR的一般概述参见《PCR方案：方法与应用指导》(PCR Protocols:A Guide toMethods and Applications)(Innis,M.,Gelfand,D.,Sninsky,J.和White,T.,编),学术出版社(Academic Press)，圣迭戈(1990)。用于从植物组织鉴定序列的合适的引物和探针来自本文提供的序列与其它相关基因的比较。
在一些实施方式中，多种植物的部分或完整基因组已经测序且具有经鉴定的开放阅读框。通过BLAST搜索，可以鉴定多种植物中的野生型PYR/PYL的编码序列。
III.制备超敏PYR/PYL受体多肽的方法
在另一方面中，本发明提供用于制备含有一个或多个氨基酸取代的ABA超敏PYR/PYL受体多肽的方法。在一些实施方式中，所述方法包括诱变野生型PYR/PYL受体并确定该诱变的PYR/PYL受体是否对ABA超敏。
突变的PYR/PYL受体多肽可通过如下方式构建：使编码对应野生型PYR/PYL受体多肽(例如，SEQ ID NO:1-119中任一项，具有SEQ ID NO:120-123中任一项的野生型PYR/PYL多肽，或本发明的突变PYR/PYL受体多肽来源的对应变体)的DNA序列突变，例如采用定点或随机诱变。编码所述野生型PYR/PYL受体多肽的核酸分子可通过本领域技术人员熟知的多种聚合酶链式反应(PCR)技术来突变。(参见例如，《PCR策略》(PCR Strategies)(M.A.Innis,D.H.Gelfand,和J.J.Sninsky编,1995,学术出版社，加州圣迭戈)，第14章；《PCR方案：方法与应用指导》(PCR Protocols:A Guide to Methods and Applications)(M.A.Innis,D.H.Gelfand,J.J.Sninsky,和T.J.White编,学术出版社,纽约州,1990)。
作为非限制性示例，诱变可采用定点诱变进行，其中对DNA模板制造点突变、插入或缺失。用于定点诱变的试剂盒是市售可得的，例如QuikChange定点诱变试剂盒(Stratagene)。简言之，待诱变的DNA模板根据生产商的说明，采用高保真DNA聚合酶(例如，Pfu TurboTM)和包含所需突变的寡核苷酸引物，通过PCR扩增。寡核苷酸的纳入产生突变的质粒，其随后可被转化进入合适的细胞(例如，细菌或酵母细胞)用于后续筛选，以确认DNA的诱变。
作为另一非限制性示例，诱变可通过易错PCR扩增(ePCR)完成，其修改了PCR反应条件(例如，采用易错聚合酶，不同的镁或锰浓度，或提供不平衡的dNTP比)以促进DNA复制中的错误率增加。用于ePCR诱变的试剂盒是市售可得的，例如PCR诱变试剂盒(Stratagene)和PCR随机诱变试剂盒(Clontech)。简言之，将DNA聚合酶(例如，Taq聚合酶)、盐(例如，MgCl2、MgSO4或MnSO4)、不平衡比例的dNTP、反应缓冲液，和DNA模板合并，然后根据生产商说明进行标准PCR扩增。在ePCR扩增之后，反应产物被克隆进入合适的载体以构建诱变的文库，其然后可被转化进入合适的细胞(例如，酵母细胞)供于后续筛选(例如，通过双杂交筛选)，如下文所述。
或者，诱变可通过重组(即DNA改组)完成。简言之，改组的突变文库通过利用体外同源重组的DNA改组，通过母DNA的随机片段化随后采用PCR重组装，来获得随机引入的点突变。进行DNA改组的方法是本领域已知的(参见例如，Stebel,S.C.等，Methods Mol Biol352:167-190(2007))。
任选地，可进行多轮诱变以提高分离的突变蛋白质的效率。因此，在一些实施方式中，可将从ePCR分离并经后续筛选的PYR/PYL突变体汇集，并用作用于后续几轮诱变的模板。
在一些实施方式中，所述变体通过使植物种子或细胞的植物接触诱变剂，通过表型或基因型选择携带本文所述的超敏PYR/PYL多肽的植物或细胞来产生。诱变剂的示例包括，例如，化学诱变剂(例如，EMS)或放射性诱变剂。具有所需突变的变体可基于基因型的表型(例如，通过采用TILLING技术)来选择。
在一些实施方式中，所述方法包括原位诱变野生型PYR/PYL受体并确定该诱变的PYR/PYL受体是否对ABA超敏。突变的PYR/PYL受体多肽可通过如下方式构建：使编码对应野生型PYR/PYL受体多肽(例如，SEQ ID NO:1-119中任一项，具有SEQ ID NO:120-123中任一项的野生型PYR/PYL多肽，或本发明的突变PYR/PYL受体多肽来源的对应变体)的DNA序列突变，例如采用定点或随机诱变。
IV.超敏PYR/PYL受体多肽的筛选
本文所述的突变PYR/PYL受体超敏性可以若干可替代的方式检测。若在酵母中表达，当合适的酵母细胞在ABA存在下生长时，大多数野生型PYR/PYL受体将仅结合至2型蛋白磷酸酶(PP2C)HAB1(或其它PP2C)。因此，在一些实施方式中，超敏性可通过如下方式来检测：确定在酵母中表达的PYR/PYL突变受体以大于酵母中表达的对照PYR/PYL受体的程度结合至酵母中的PP2C并使PP2C失活的能力。在一些实施方式中，所述超敏突变PYR/PYL受体包含突变，相较于相同浓度的ABA存在下的野生型或其它对照PYR/PYL受体，所述突变导致突变的受体在磷酸酶试验中于ABA的存在下以至少约10％、至少约20％、至少约30％、至少约40％、至少约50％、至少约60％、至少约70％、至少约80％或更多抑制PP2C活性。可采用低nM至第μM范围的若干测试浓度来推断IC50值，并且，超敏突变的IC50值显著低于合适的野生型对照。
或者，可采用基于细胞或基于植物的筛选方法。例如，可采用天然表达野生型PYR/PYL受体多肽或重组表达野生型或突变的PYR/PYL受体多肽的细胞。在一些实施方式中，所用细胞是植物细胞、动物细胞、细菌细胞、真菌细胞，包括但不限于酵母细胞、昆虫细胞，或哺乳动物细胞。一般而言，所述筛选方法涉及：比较ABA存在下，突变的PYR/PYL受体多肽的活性与野生型PYR/PYL受体多肽的活性，例如，通过比较野生型和突变PYR/PYL受体-表达细胞或植物中的ABA-调节的基因表达。
一个示例性的试验涉及测试在ABA存在下，突变的PYR/PYL受体能否结合至2型蛋白磷酸酶(PP2C)(例如，与ABI1(HAB1)同源)。结合试验可涉及：使突变的PYR/PYl受体多肽与PP2C接触，并允许接触足够的时间以供PYR/PYL受体与PP2C形成结合复合物。形成的任何结合复合物可采用任何多种已建立的分析技术检测。蛋白质结合试验包括但不限于，检测共沉淀或非变性SDS-聚丙烯酰胺凝胶上的共迁移，以及Western印迹上的共迁移的方法(参见例如，Bennet,J.P.和Yamamura,H.I.(1985)"神经递质、激素或药物受体结合方法(Neurotransmitter,Hormone or Drug Receptor Binding Methods)"，刊于《神经递质受体结合》(Neurotransmitter Receptor Binding)(Yamamura,H.I.等编)，第61-89页。其它结合试验涉及质谱或NMR技术的利用，以鉴定结合至PYR/PYL多肽的分子。用于此类试验的PYR/PYL多肽蛋白质可以是天然表达、克隆或合成的。
在一些实施方式中，可采用哺乳动物或酵母双杂交方法(参见例如，Bartel,P.L.等.Methods Enzymol，254:241(1995))来鉴定在细胞中一起表达时相互作用或结合的多肽或其它分子。在一些实施方式中，在PYR/PYL多肽和PP2C多肽之间的双杂交试验中鉴定超敏PYR/PYL多肽，其中，所述PYR/PYL多肽和PP2C在ABA存在下结合。
在另一个示例性试验中，突变的PYR/PYL受体多肽的基础活性水平(即，在不存在ABA时的活性水平)可采用酶促磷酸酶试验来测定，其中，PYR/PYL受体和PP2C在ABA存在下孵育。在该类型试验中，在ABA存在下，磷酸酶活性的降低程度高于对照PYR/PYL受体的情况指示超敏PYR/PYL受体。磷酸酶活性的降低可采用本领域已知的任何检测试剂来确定并定量，例如，比色检测试剂例如对硝基苯磷酸盐。
通过任何前述筛选方法初始鉴定的超敏PYR/PYL受体多肽还可经测试以验证该超敏PYR/PYL受体多肽的表面活性和/或确定其它生物效应。在一些情况中，测试该PYR/PYL受体多肽的影响植物胁迫(例如，干旱耐受和/或高盐耐受)、种子发芽，或受ABA影响的另一表型的能力。多种此类试验和表型是本领域已知的，并可根据本发明方法应用。
V.重组表达载体
一旦获得编码突变的PYR/PYL受体多肽多核苷酸，其还可用以制备用于在转基因植物中通过异源性启动子引导来表达突变的PYR/PYL受体多肽的表达盒。突变的PYR/PYL多核苷酸的增加的表达可用于，例如，产生选择性地活化PYR/PYL受体的植物，因此增强胁迫耐受。
可采用本领域熟知的多种手段中的任一种来驱动植物中突变的PYR/PYL活性或表达。可靶标任何器官，例如芽滋养型器官/结构(例如叶、茎和块茎)、根、花和花的器官/结构(例如苞、萼片、花瓣、雄蕊、心皮、花药和胚珠)、种子(包括胚、胚乳，和种皮)和果。或者，突变的PYR/PYL多核苷酸可在一种或多种器官内的某些细胞和/或组织类型中特异性表达(例如，采用保卫细胞特异性启动子的叶中的保卫细胞)。或者，该突变的PYR/PYL多核苷酸可组成型表达(例如，采用CaMV 35S启动子)。
为了在上述技术中采用突变的PYR/PYL受体多肽的多核苷酸序列，制备适于转化植物细胞的重组DNA载体。用于转化广泛的高等植物物种的技术是熟知的且描述于科技文献中。参见例如，Weising等Ann.Rev.Genet.22:421-477(1988)。编码突变的PYR/PYL受体多肽的DNA序列优选联合转录和翻译起始调节序列，其将引导序列在转化植物的意图的组织中从基因的转录。
例如，植物启动子片段可用于引导突变的PYR/PYL多核苷酸在再生植物的所有组织中的直接表达。所述启动子在本文中称作"组成型"启动子，且在大多数环境条件和发育或细胞分化状态下具有活性。组成型启动子的示例包括花椰菜花叶病病毒(CaMV)35S转录起始区域、源自根癌土壤杆菌(Agrobacterium tumafaciens)T-DNA的1'-或2'-启动子，和来自本领域技术人员已知的多种植物基因的其它转录起始区域。
或者，所述植物启动子可引导突变的PYR/PYL受体蛋白在特定组织中的表达(组织特异性启动子)或在其它情况下可以是在更多准确环境控制下的表达(诱导型启动子)。发育控制下的组织特异性启动子的示例包括仅在某些组织(例如叶或保卫细胞)中起始转录的启动子(包括但不限于描述于以下文献中的那些：WO 2005/085449；美国专利号6,653,535；Li等，Sci China C Life Sci.2005年4月；48(2):181-6；Husebye等，Plant Physiol，2002年4月，第128卷，第1180-1188页；和Plesch等.,Gene,第249卷，第1期，2000年5月16日，第83-89(7)页)。可通过诱导型启动子影响转录的环境条件的示例包括厌氧条件、升高的温度，或光照。
如果希望正确的蛋白质表达，应在编码区域的3'端包括聚腺苷酸化区域。聚腺苷酸化区域可源自天然产生的PYR/PYL基因，来自多种其它植物基因，或来自T-DNA。
含有所述序列(例如，启动子或PYR/PYL编码区域)的载体将通常包含标志物基因，其提供植物细胞上选择性表型。例如，该标志物可编码杀生物剂(biocide)抗性，特别是抗生素抗性，例如对卡那霉素、G418、博来霉素、潮霉素的抗性，或除草剂抗性，例如对氯磺隆或巴斯达(Basta)的抗性。
在一些实施方式中，所述突变的PYR/PYL核酸序列在植物细胞中重组表达。可制备适于转化植物细胞的多种不同表达构建体，例如表达盒和载体。用于转化广泛的高等植物物种的技术是熟知的且描述于科技文献中。参见例如，Weising等Ann.Rev.Genet.22:421-477(1988)。编码PYR/PYL蛋白质的DNA序列可联合顺式作用(启动子)和反式作用(增强子)转录调节序列，以引导在转化植物的意图的组织中的时间控制、组织类型和水平转录。还可采用翻译控制元件。
本发明的实施方式还提供操作性地连接至启动子的突变的PYR/PYL核酸，其在一些实施方式中，能够驱动植物中PYR/PYL编码序列的转录。所述启动子可以，例如，源自植物或病毒来源。所述启动子可以是，例如，组成型活性、诱导型或组织特异性启动子。在本发明的重组表达盒、转基因、载体的构建中，可选择不同启动子并用于差异性地引导基因表达，例如，在植物或动物的一些或全部组织中。
组成型启动子
片段可用于导向突变的PYR/PYL核酸在全部转化细胞或组织中的表达，例如再生植物的那些。术语"组成型调节元件"表示这样的调节元件，其提供相对独立于其中表达所述组成型调节元件的细胞或组织类型的操作性连接的核酸分子上的表达水平。植物中表达的组成型调节元件一般广泛表达于大量细胞和组织类型中。驱动生理条件下的连续表达的启动子称作"组成型"启动子，并且在大多数环境条件和发育或细胞分化状态下具有活性。
可用于转基因植物中的异位表达的多种组成型调节元件是本领域熟知的。例如，花椰菜花叶病病毒35S(CaMV 35S)启动子，是成熟表征的组成型调节元件，其在所有植物组织中产生高水平的表达(Odell等，Nature 313:810-812(1985))。CaMV 35S启动子可能是特别有用的，这归因于其在多种不同的植物物种中的活性(Benfey和Chua，Science 250:959-966(1990)；Futterer等，Physiol.Plant 79:154(1990)；Odell等，同上，1985)。其中固有启动子元件已重复的串联35S启动子提供高于未经修饰的35S启动子的表达水平(Kay等，Science 236:1299(1987))。其它有用的组成型调节元件包括，例如，花椰菜花叶病病毒19S启动子；玄参花叶病病毒启动子；和，胭脂碱合酶(nos)基因启动子(Singer等，PlantMol.Biol.14:433(1990)；An，Plant Physiol.81:86(1986))。
包括供于在单子叶植物中充分表达的那些的额外的组成型调节元件也是本领域已知的，例如，pEmu启动子和基于稻肌动蛋白-1 5'区域的启动子(Last等，Theor.Appl.Genet.81:581(1991)；Mcelroy等，Mol.Gen.Genet.231:150(1991)；Mcelroy等，Plant Cell 2:163(1990))。联合来自不同基因的元件的嵌合调节元件也可用于异位表达编码突变的PYR/PYL受体蛋白的核酸分子(Comai等，Plant Mol.Biol.15:373(1990))。
组成型启动子的其它示例包括：源自根癌土壤杆菌(Agrobacteriumtumafaciens)的T-DNA的1'-或2'-启动子(参见例如，Mengiste(1997)同上；O'Grady(1995)Plant Mol.Biol.29:99-108)；肌动蛋白启动子，例如拟南芥(Arabidopsis)肌动蛋白基因启动子(参见例如，Huang(1997)Plant Mol.Biol.1997 33:125-139)；醇脱氢酶(Adh)基因启动子(参见例如，Millar(1996)Plant Mol.Biol.31:897-904)；来自拟南芥的ACT11(Huang等Plant Mol.Biol.33:125-139(1996))，来自拟南芥的Cat3(GenBank编号U43147，Zhong等，Mol.Gen.Genet.251:196-203(1996))，来自欧洲油菜(Brassica napus)的编码硬脂酰-酰基载体蛋白去饱和酶的基因(Genbank编号X74782，Solocombe等PlantPhysiol.104:1167-1176(1994))，来自玉米的GPc1(GenBank编号X15596，Martinez等J.Mol.Biol 208:551-565(1989))，来自玉米的Gpc2(GenBank编号U45855，Manjunath等，Plant Mol.Biol.33:97-112(1997))，本领域技术人员已知的来自多种植物基因的其它转录起始区域。还参见Holtorf Plant Mol.Biol.29:637-646(1995)。
诱导型启动子
或者，植物启动子可引导突变的PYR/PYL多核苷酸在变化的环境条件或发育条件的影响下的表达。可通过诱导型启动子影响转录的环境条件的示例包括厌氧条件、升高的温度、干旱或光照。所述启动子本文中称作"诱导型"启动子。在一些实施方式中，诱导型启动子通过一种或多种环境应激源，包括但不限于，干旱、冷冻和高盐诱导。例如，本发明可纳入干旱特异性启动子，例如玉米的干旱诱导型启动子(例如，玉米rab17干旱诱导型启动子(Vilardell等(1991)Plant Mol.Biol.17:985-993；Vilardell等(1994)PlantMol.Biol.24:561-569))；或者来自马铃薯(Kirch(1997)Plant Mol.Biol.33:897-909)或来自拟南芥(例如，rd29A启动子(Kasuga等(1999)Nature Biotechnology17:287-291)的冷冻、干旱和高盐诱导型启动子。其它环境胁迫诱导型启动子包括来自如下基因的启动子：稻中的Rab21、Wsi18、Lea3、Uge1、Dip1和R1G1B(Yi等(2010)Planta 232:743-754)。
在一些实施方式中，植物启动子是胁迫诱导型启动子(例如，干旱-、冷冻-或盐诱导型启动子)，其包含脱水-响应性元件(DRE)和/或ABA-响应性元件(ABRE)，包括但不限于rd29A启动子。
或者，可采用在接触植物激素，例如植物生长素，之后呈诱导型的植物启动子来表达突变的PYR/PYL多核苷酸。例如，本发明可采用大豆中的植物生长素-响应性元件E1启动子片段(AuxREs)(橹豆L.)(Liu(1997)Plant Physiol.115:397-407)；植物生长素-响应性拟南芥GST6启动子(还响应水杨酸和过氧化氢)(Chen(1996)Plant J.10:955 966)；来自烟草的植物生长素诱导型parC启动子(Sakai(1996)37:906 913)；植物生物素响应元件(Streit(1997)Mol.Plant Microbe Interact.10:933 937)；和，响应胁迫激素脱落酸的启动子(Sheen(1996)Science 274:1900 1902)。
可应用至植物的接触化学品试剂(例如除草剂或抗生素)之后呈诱导型的植物启动子也可用于表达突变的PYR/PYL多核苷酸。例如，可采用通过苯磺酰胺除草安全剂活化的玉米In2-2启动子(De Veylder(1997)Plant Cell Physiol.38:568 577)；不同除草安全剂的应用诱导不同基因表达模式，包括在根、排水器和芽顶端分生组织中的表达。PYR/PYL编码序列还可受例如四环素诱导型启动子的控制，例如，如所述用于包含燕麦L(oat)精氨酸脱羧酶基因的转基因烟草植物(Masgrau(1997)Plant J.11:465-473)；或，水杨酸-响应性元件(Stange(1997)Plant J.11:1315-1324；Uknes等，Plant Cell 5:159-169(1993)；Bi等，Plant J.8:235-245(1995))。
有用的诱导型调节元件的示例包括铜诱导型调节元件(Mett等，Proc.Natl.Acad.Sci.USA 90:4567-4571(1993)；Furst等，Cell 55:705-717(1988))；四环素和氯-四环素诱导型调节元件(Gatz等，Plant J.2:397-404(1992)；等，Mol.Gen.Genet.243:32-38(1994)；Gatz，Meth.Cell Biol.50:411-424(1995))；蜕化素诱导型调节元件(Christopherson等，Proc.Natl.Acad.Sci.USA89:6314-6318(1992)；Kreutzweiser等，Ecotoxicol.Environ.Safety 28:14-24(1994))；热激诱导型调节元件(Takahashi等，Plant Physiol.99:383-390(1992)；Yabe等，Plant Cell Physiol.35:1207-1219(1994)；Ueda等，Mol.Gen.Genet.250:533-539(1996))；和，lac操作子元件，其与组成型表达lac遏制物联用以提供，例如，IPTG诱导型表达(Wilde等，EMBO J.11:1251-1259(1992))。可用于本发明的转基因植物的诱导型调节元件还可以是，例如，源自菠菜亚硝酸盐还原酶基因的亚硝酸盐诱导型启动子(Back等，Plant Mol.Biol.17:9(1991))或光诱导型启动子，例如与RuBP羧酶小亚基或LHCP基因家族相关联(Feinbaum等，Mol.Gen.Genet.226:449(1991)；Lam和Chua，Science 248:471(1990))。
组织特异性启动子
或者，植物启动子可引导突变的PYR/PYL多核苷酸在特定组织中的表达(组织特异性启动子)。组织特定启动子是转录控制元件，其仅在具体细胞或组织中于植物发育过程中的特定时间具有活性，例如在滋养型组织或再生组织中。
受发育控制的组织特异性启动子的示例包括仅(或主要仅)在某些组织，例如滋养型组织，例如，根或叶，或再生组织，例如果、胚珠、种子、花粉、雌蕊(pistol)、花或任何胚组织，或表皮或叶肉中起始转录的启动子。再生组织特异性启动子可具有，例如，胚珠特异性、胚特异性、胚乳特异性、珠被特异性、种子和种皮特异性、花粉特异性、花瓣特异性、萼片特异性或它们的一些组合。在一些实施方式中，所述启动子具有细胞-类型特异性，例如，保卫细胞特异性。
表皮特异性启动子包括，例如，拟南芥LTP1启动子(Thoma等(1994)PlantPhysiol.105(1):35-45)，CER1启动子(Aarts等(1995)Plant Cell 7:2115-27)，和CER6启动子(Hooker等(2002)Plant Physiol 129:1568-80)，和直系同源番茄LeCER6(Vogg等(2004)J.Exp Bot.55:1401-10)。
保卫细胞特异性启动子包括，例如，DGP1启动子(Li等(2005)Science China CLife Sci.48:181-186)。
其它组织特异性启动子包括种子启动子。合适的种子特异性启动子源自如下基因：来自玉米的MAC1(Sheridan(1996)Genetics 142:1009-1020)；来自玉米的Cat3(GenBank编号L05934，Abler(1993)Plant Mol.Biol.22:10131-1038)；来自拟南芥的vivparous-1(Genbank编号U93215)；来自拟南芥的atmyc1(Urao(1996)PlantMol.Biol.32:571-57；Conceicao(1994)Plant 5:493-505)；来自欧洲油菜的(napA)(GenBank编号J02798，Josefsson(1987)JBL 26:12196-1301)；和，来自欧洲油菜的油菜籽蛋白基因家族(Sjodahl(1995)Planta 197:264-271)。
特别在滋养型组织，例如叶、茎、根和块根中具有活性的多种启动子也可用以表达编码突变的PYR/PYL受体多肽的多核苷酸。例如，可采用控制马铃薯糖蛋白(马铃薯块茎的主要贮藏蛋白)的启动子，参见例如，Kim(1994)Plant Mol.Biol.26:603-615；Martin(1997)Plant J.11:53-62。也可使用显示根中高活性的来自土壤杆菌rhizo基因的ORF13启动子(Hansen(1997)Mol.Gen.Genet.254:337-343。其他有用的滋养型组织特异性启动子包括：编码来自大芋头(Colocasia esculenta L.Schott)球茎蛋白质家族的球蛋白的基因tarin的tarin启动子(Bezerra(1995)Plant Mol.Biol.28:137-144)；在芋头球茎发育过程中具有活性的仙茅甜蛋白启动子(de Castro(1992)Plant Cell 4:1549-1559)和烟草根特异性基因TobRB7的启动子，其表达位于根分生组织和未成熟中央柱状区域中(Yamamoto(1991)Plant Cell 3:371-382)。
也可采用叶特异性启动子，例如二磷酸核酮糖羧化酶(RBCS)启动子。例如，番茄RBCS1、RBCS2和RBCS3A基因表达于叶和光-生长型幼苗，仅RBCS1和RBCS2表达于发育中的番茄果(Meier(1997)FEBS Lett.415:91-95)。可采用几乎仅在叶片和叶鞘的叶肉细胞中以高水平表达的二磷酸核酮糖羧化酶启动子，根据Matsuoka所述(1994)Plant J.6:311-319。另一叶特异性启动子是集光叶绿素a/b结合蛋白质基因启动子，参见例如，Shiina(1997)Plant Physiol.115:477-483；Casal(1998)Plant Physiol.116:1533-1538。拟南芥myb相关的基因启动子(Atmyb5)(描述于Li(1996)FEBS Lett.379:117-121)是叶特异性的。Atmyb5启动子表达于发育中的叶毛状体、托叶和幼莲座状叶和茎叶边缘上的表皮细胞，和未成熟种子中。Atmyb5mRNA在受精和胚发育16细胞阶段之间出现，并且持续超过心期(heart stage)。也可采用玉米中鉴定的叶启动子，根据Busk(1997)Plant J.11:1285-1295所述。
另一类可用的滋养型组织特异性启动子是分生组织(根尖和芽顶点)启动子。例如，可采用"SHOOTMERISTEMLESS"和"SCARECROW"启动子，其在发育中的芽或根顶端分生组织中具有活性，描述于Di Laurenzio(1996)Cell86:423-433；和，Long(1996)Nature 379:66-69。另一可用的启动子是控制3-羟基-3-甲基戊二酰辅酶A还原酶HMG2基因的表达的启动子，其表达限制于分生组织和花(柱头分泌区、成熟花粉粒、雌蕊维管组织，和受精的胚珠)组织(参见例如，Enjuto(1995)Plant Cell.7:517-527)。还可采用来自玉米和其它物种的kn1相关的基因，其显示分生组织特异性表达，参见例如，Granger(1996)PlantMol.Biol.31:373-378；Kerstetter(1994)Plant Cell 6:1877-1887；Hake(1995)Philos.Trans.R.Soc.Lond.B.Biol.Sci.350:45-51。例如，拟南芥KNAT1启动子(参见例如，Lincoln(1994)Plant Cell 6:1859-1876)。
本领域技术人员应理解，组织特异性启动子可驱动操作性连接的序列在非靶标组织的组织中表达。因此，本文所用的组织特异性启动子是优先驱动靶标组织中的表达，但还可导致其它组织中的一些表达的那些。
在另一个实施方式中，所述突变的PYR/PYL多核苷酸通过转座元件表达。这允许组成型活性多肽的组成型但周期性且稀少的表达。本发明还提供源自病毒的组织特异性启动子的应用，包括，例如，烟草花叶病毒亚基因组启动子(Kumagai(1995)Proc.Natl.Acad.Sci.USA 92:1679-1683；稻东格鲁杆状病毒(RTBV)，其仅在受感染的稻植物中的韧皮部细胞中表达，其中，其启动子驱动强韧皮部特异性报告基因表达；木薯脉花叶病毒(CVMV)启动子，其在维管元件、叶叶肉细胞和根尖中活性最高(Verdaguer(1996)PlantMol.Biol.31:1129-1139)。
VI.含有超敏突变的植物的生成
在另一方面中，本发明提供转基因植物，其含有用于在所述植物中表达本文所述的超敏PYR/PYL受体蛋白的重组表达盒。在一些实施方式中，产生的转基因植物包含源自不同于所述转基因植物的物种的物种的多核苷酸的完全或部分序列。应理解，转基因植物涵盖其中引入所述表达盒的植物或植物细胞，以及包含所述表达盒的所述植物或植物细胞的后裔，包括具有稳定地整合至染色体中的表达盒的后裔。
可通过多种常规技术将含有由异源性启动子驱动的PYR/PYL编码序列的重组表达载体引入所需植物宿主的基因组。例如，可利用如植物细胞原生质体的电穿孔和微注射等技术将DNA构建体直接引入植物细胞的基因组DNA，或者可利用生物射弹方法如DNA粒子轰击将DNA构建体直接引入植物组织。或者，DNA构建体可与合适的T-DNA侧接区域组合并引入常规的根癌土壤杆菌(Agrobacterium tumefaciens)宿主载体。当细胞被根癌土壤杆菌感染时，该细菌宿主的病毒性功能将引导构建体和毗邻标志物插入植物细胞DNA。尽管本发明涵盖组成型活性PYR/PYL受体的瞬时表达，一般而言，本发明的构建的表达将来自表达盒插入植物基因组，例如，从而至少一些植物后代还包含该整合的表达盒。
微注射技术也可用于该目的。这些技术是本领域熟知的，并且在文献中有详细描述。利用聚乙二醇沉淀的DNA构建体的引入描述于Paszkowski等EMBO J.3:2717-2722(1984)。电穿孔技术描述于Fromm等Proc.Natl.Acad.Sci.USA82:5824(1985)。射弹转化技术描述于Klein等Nature 327:70-73(1987)。
科技文献中描述由根癌土壤杆菌介导的转化技术，包括二元载体的卸装和使用。参见例如，Horsch等Science 233:496-498(1984)，和Fraley等Proc.Natl.Acad.Sci.USA80:4803(1983)。
上述转化技术中任一种衍生的转化植物细胞可培养再生为完整植物，其具有转化的基因型和所需的表型，例如增强的非生物胁迫抗性。此类再生技术有赖于组织培养生长培养基中某些植物激素的控制，通常有赖于和所需核苷酸序列一同引入的抗微生物剂和/或除草剂标志物。从培养的原生质体进行植物再生的内容描述于Evans等收录于《植物细胞培养手册》(Handbook of Plant Cell Culture)第124-176页的"原生质体分离与培养(Protoplasts Isolation and Culture)"，麦克米兰出版公司(Macmillian PublishingCompany)，纽约，1983；和《植物的再生、植物原生质体》(Binding,Regeneration ofPlants,Plant Protoplasts)，第-73-73页，CRC出版社，博卡拉顿，1985。再生可还获自植物愈伤组织、外植体、器官或其部分。所述再生技术一般描述于Klee等Ann.Rev.of PlantPhys.467-486(1987)。
本领域技术人员认识到，表达盒稳定整合入转基因植物中并证实为可操作后，可通过有性杂交将其引入其它植物。可使用任意数量的标准育种技术，这取决于待杂交物种。
本发明的表达盒可用以对基本任何植物提供非生物胁迫抗性。因此，本发明具有对于广泛植物的应用，包括来自以下属的物种：天门冬属(Asparagus)、颠茄属(Atropa)、燕麦属(Avena)、芸苔属(Brassica)、柑橘属(Citrus)、西瓜属(Citrullus)、辣椒属(Capsicum)、黄瓜属(Cucumis)、南瓜属(Cucurbita)、胡萝卜属(Daucus)、草莓属(Fragaria)、大豆属(Glycine)、棉属(Gossypium)、向日葵属(Helianthus)、萱草属(Heterocallis)、大麦属(Hordeum)、天仙子属(Hyoscyamus)、莴苣属(Lactuca)、亚麻属(Linum)、黑麦草属(Lolium)、番茄属(Lycopersicon)、苹果属(Malus)、木薯属(Manihot)、唇形科牛至属(Majorana)、苜蓿属(Medicago)、烟草属(Nicotiana)、稻属(Oryza)、黍属(Panieum)、狼尾草属(Pannesetum)、鳄梨属(Persea)、豌豆属(Pisum)、梨属(Pyrus)、李属(Prunus)、萝卜属(Raphanus)、黑麦属(Secale)、千里光属(Senecio)、白芥属(Sinapis)、茄属(Solanum)、高粱属(Sorghum)、胡卢巴属(Trigonella)、小麦属(Triticum)、葡萄属(Vitis)、豇豆属(Vigna)和玉蜀黍属(Zea)。在一些实施方式中，所述植物选自下组：水稻、玉米、小麦、大豆、棉、油菜、草皮草和苜蓿。在一些实施方式中，所述植物是观赏植物。在一些实施方式中，所述植物是产蔬菜或产果植物。
本领域技术人员应理解，可采用多种植物物种作为模型来预测在其它植物中的转基因表达的表型作用。例如，已公认烟草(Nicotiana)和拟南芥植物是转基因表达(尤其在其它双子叶植物中的)有用模型。
在一些实施方式中，相较于除了超敏PYR/PYL受体多肽的表达以外其它情况相同的植物，本发明植物具有增强的ABA介导的表型，例如增强的种子休眠。本领域技术人员应理解，ABA是充分研究的植物激素，并且ABA介导许多特性变化，其中任何一项均可被监测以确定表型变化。在一些实施方式中，增强的ABA介导的表型通过改变的种子发芽时间或改变的胁迫(例如，干旱、冷冻和/或盐)耐受来显示。
非生物胁迫抗性可根据多种熟知技术中的任一种测试。例如，对于干旱耐受，可使植物在其中向植物提供少于最优水分的条件下生长。干旱抗性可通过多种标准检测法包括膨压、生长、产量等中的任一种来确定。在一些实施方式中，如果在确定的时程(例如，5，6，7，8，9，10，11，12，13，14天或更久，例如3、4、5周或更久的时程)中，相较于非转基因对照植物，转基因植物中的膨胀损失减少至少约10％、15％、20％、25％、30％、40％、50％、60％、70％、80％、90％或更多，则表达本文所述的突变的PYR/PYL受体的转基因植物具有增强的干旱耐受。
在一些实施方式中，所述增强的ABA介导的表型是对中等或高盐度的增强的耐受。盐度耐受可通过多种标准检测法中的任一种确定，包括发芽、生长、产量或植物存活、叶损伤、叶绿素早熟损失等.在一些实施方式中，如果在确定的时程(例如，5、6、7、8、9、10、11、12、13、14天或更久，例如，3、4、5周或更久)中，相较于非转基因对照植物，转基因植物在中等-盐或高-盐条件(例如，约50mM、100mM、150mM、200mM、250mM、300mM NaCl或更高)的存活增加至少约10％、15％、20％、25％、30％、40％、50％、60％、70％、80％、90％或更多，则表达本文所述的突变的PYR/PYL受体的转基因植物具有增强的盐耐受。
目前，采用CRISPR/Cas9基因组编辑方法，植物基因操作可在非转基因生物体中准确调整。在该细菌抗病毒和转录调节系统中，两个小RNA–CRISPR-RNA(crRNA)和反式作用crRNA(tracrRNA)的复合物–将核酸酶(Cas9)引导至与crRNA互补的特定DNA序列(Jinek，M.等Science 337，816–821(2012))。这些RNA与Cas9的结合涉及RNA的特异性序列和二级结构。这两个RNA组分可被简化成单一元件，单一导向-RNA(sgRNA)，其从包含由用户定义的靶序列的盒转录(Jinek，M.等Science 337，816–821(2012))。该系统已用于人、斑马鱼、果蝇、小鼠、线虫、细菌、酵母和植物中的基因组编辑(Hsu，P.D.等，Cell 157，1262–1278(2014))。在该系统中，核酸酶在sgRNA编程的靶区域处产生双链断裂。这些可通过非同源重组修复，其常产生失活性突变。所述断裂还可通过同源重组修复，其允许系统用于基因靶向的基因替代(Li，J.-F.等Nat.Biotechnol.31，688–691，2013；Shan，Q.等Nat.Biotechnol.31，686–688，2013)。本申请所述的超敏突变可采用CAS9/CRISPR系统引入植物。
因此，在一些实施方式中，作为产生转基因植物的替代，可原位改变植物或植物细胞中的原始PYR/PYR编码序列，以产生携带编码本文所述的超敏PYR/PYL多肽的多核苷酸的植物或植物细胞。例如，在一些实施方式中，采用CRISPR技术以将一种或多种核苷酸变化原位引入PYR/PYL编码序列，以改变合适的密码子，以产生对应于SEQ ID NO:1的F61X、V81X、I110X或V163X的变化。CRISPR/Cas系统已经改良用于原核和真核系统，供于基因组编辑和转录调节。“CRISPR/Cas”系统指，用于抵抗外来核酸的普遍类型的细菌系统。CRISPR/Cas系统存在于大量真细菌和古生菌生物体。CRISPR/Cas系统包括I、II和III型亚型。野生型II型CRISPR/Cas系统利用RNA介导的与导向和活化RNA复合的核酸酶Cas9，以识别和切割外来核酸。Cas9同系物存在于多种真细菌中，包括，但不限于如下分类的细菌：放线菌(Actinobacteria)、产水菌(Aquificae)、拟杆菌-绿菌(Bacteroidetes-Chlorobi)、衣原体-疣微菌(Chlamydiae-Verrucomicrobia)、绿弯菌(Chlroflexi)、蓝细菌(Cyanobacteria)、厚壁菌(Firmicutes)、变形菌(Proteobacteria)、螺旋体(Spirochaetes)和热袍菌(Thermotogae)。示例性的Cas9蛋白是酿脓链球菌(Streptococcus pyogenes)Cas9蛋白。其它Cas9蛋白及其同系物描述于，例如，Chylinksi等，RNA Biol.2013年5月；10(5):726–737；Nat.Rev.Microbiol.2011年6月；9(6):467-477；Hou等,Proc Natl Acad Sci U S A.2013年9月；24；110(39):15644-9；Sampson等,Nature.2013年5月；9；497(7448):254-7；和Jinek等,Science.2012年8月；17；337(6096):816-21。
因此，在一个方面中，提供利用CRISPR/CAS9以将至少一种本文所述的突变引入植物细胞的方法。在一些实施方式中，提供改变植物中编码PYR/PYL多肽的(例如，原始)核酸的方法。在一些实施方式中，所述方法包括将工程改造的、非天然产生的规律成簇的间隔短回文重复(CRISPR)—CRISPR相关联的(Cas)(CRISPR-Cas)系统引入包含且表达具有编码PYR/PYL多肽的靶核酸的DNA分子的植物细胞。在一些实施方式中，所述CRISPR-Cas系统包含一种或多种载体，其包含：a)在植物细胞中可操作的第一调节元件，其操作性地连接至编码与靶序列杂交的CRISPR-Cas系统导向RNA的至少一个核苷酸序列，和b)在植物细胞中可操作的第二调节元件，其操作性地连接至编码II型Cas9蛋白的核苷酸序列，其中组分(a)和(b)位于系统的相同或不同载体上，由此，该导向RNA靶向靶序列，并且Cas9蛋白切割DNA分子，由此至少一种本文所述的超敏突变被引入编码该PYR/PYL多肽的靶核酸。在一些实施方式中，所述PRY/PYL多肽选自SEQ ID NO:1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、49、50、51、52、53、54、55、56、57、58、59、60、61、62、63、64、65、66、67、68、69、70、71、72、73、74、75、76、77、78、79、80、81、82、83、84、85、86、87、88、89、90、91、92、93、94、95、96、97、98、99、100、101、102、103、104、105、106、107、108、109、110、111、112、113、114、115、116、117、118或119中的任一种或基本相同的多肽。在一些实施方式中，所述植物选自以下属：天门冬属(Asparagus)、颠茄属(Atropa)、燕麦属(Avena)、芸苔属(Brassica)、柑橘属(Citrus)、西瓜属(Citrullus)、辣椒属(Capsicum)、黄瓜属(Cucumis)、南瓜属(Cucurbita)、胡萝卜属(Daucus)、草莓属(Fragaria)、大豆属(Glycine)、棉属(Gossypium)、向日葵属(Helianthus)、萱草属(Heterocallis)、大麦属(Hordeum)、天仙子属(Hyoscyamus)、莴苣属(Lactuca)、亚麻属(Linum)、黑麦草属(Lolium)、番茄属(Lycopersicon)、苹果属(Malus)、木薯属(Manihot)、唇形科牛至属(Majorana)、苜蓿属(Medicago)、烟草属(Nicotiana)、稻属(Oryza)、黍属(Panieum)、狼尾草属(Pannesetum)、鳄梨属(Persea)、豌豆属(Pisum)、梨属(Pyrus)、李属(Prunus)、萝卜属(Raphanus)、黑麦属(Secale)、千里光属(Senecio)、白芥属(Sinapis)、茄属(Solanum)、高粱属(Sorghum)、胡卢巴属(Trigonella)、小麦属(Triticum)、葡萄属(Vitis)、豇豆属(Vigna)和玉蜀黍属(Zea)。在一些实施方式中，所述植物选自下组：水稻、玉米、小麦和大豆。在一些实施方式中，引入靶核酸的超敏突变是(对应于其在拟南芥PYR1(SEQ ID NO:1)中的位置)：F61、V81、I110、E141或A160或其组合。在一些实施方式中，无其它突变被引入所述靶核酸。还提供获自上述方法的植物或植物细胞。所述植物将包含编码所述超敏PYR/PYL多肽的非天然产生的核酸序列。
VII.PYR/PYL融合蛋白
在一些实施方式中，本文所述的超敏PYR/PYL多肽以融合蛋白形式提供，即，与一种或多种融合伴侣的翻译融合物。在一些实施方式中，超敏PYR/PYL多肽与转录活化或调节结构域融合。所述结构域的非限制性示例是VP16或VP64。所述融合蛋白还可包含核定位信号序列，从而该融合蛋白在真核宿主细胞中翻译时定位至细胞核。还提供编码所述融合蛋白的多核苷酸以及包含和表达所述多核苷酸的宿主细胞。在所述情况中，所述多核苷酸对于该宿主细胞是异源性的，即，不是天然产生的，例如转化进入所述细胞。
所述融合蛋白可用于，例如，在和与ABA INSENSITIVE 1(ABI1)或对于结合ABA的PYR/PYL蛋白具有特异性结合亲和性的其它蛋白质融合的序列特异性DNA结合结构域共表达时，控制所述细胞中的真核基因表达。示例性的序列特异性DNA结合结构域包括但不限于：锌指蛋白质、TALENS、转录因子DNA结合结构域，和失活的Cas9(dCas9)的RNA-导向的DNA结合结构域。当两种融合蛋白在ABA存在下于细胞中共表达时，这两种融合蛋白将共定位，归因于ABA1与ABA结合型PYR/PYL蛋白质的结合，由此产生靶标启动子附近的转录活化或调节结构域，由此调节基因表达。系统及其在基因调节中的应用的示例描述于，例如，Konermann等，Nature 500:472-476(2013)和Liang等，Science第4卷第164期(2011)。
RNA引导的基因组修饰
在一个方面中，本文提供在本文所述的植物细胞基因组中的PYR/PYL突变靶位点原位引入突变的方法。例如，在一些实施方式中，所述PYR/PYL突变靶位点包含编码PYL-E的V89或PYL的E149的核酸。在某些实施方式中，所述方法包括引入所述植物细胞：1)CRISPR核糖核酸(crRNA)，其包括基本相同于SEQ ID NO:363、364、365、366、367或369的序列；2)反式作用核糖核酸(tracRNA)；3)核酸酶(例如，Cas9)；和，4)可经历包含所述突变的同源重组的修复核酸。根据主题方法，所述crRNA和tracRNA将核酸酶导向至植物细胞基因组中的PYR/PYL突变靶位点。在其招募后，该核酸酶(例如，Cas9)在PYR/PYL突变靶位点处产生双链断裂。该PYR/PYL突变靶位点处的双链断裂促进包含所述突变的修复核酸与包括所述PYR/PYL突变靶位点的植物细胞基因组区域的同源重组，由此在PYR/PYL突变靶位点处引入所述突变。
突变可采用主题方法引入任何合适的植物细胞。在一些实施方式中，所述植物细胞是植物胚。在某些实施方式中，所述植物细胞是玉米植物细胞。
可采用本领域已知的任何合适的方法将所述方法的各组分引入植物细胞。在某些实施方式中，所述crRNA和tracRNA以包含编码所述crRNA和/或traRNA的多核苷酸(即，DNA)的表达盒的形式引入所述细胞。在一些实施方式中，所述表达盒包括RNA聚合酶启动子，其操作性地连接至编码所述crRNA和/或traRNA的多核苷酸，由此允许crRNA和/或traRNA的转录。在一些实施方式中，所述Cas9以包含操作性地连接至编码Cas9的多核苷酸的启动子的表达载体的形式引入所述细胞。可采用任何合适的启动子，包括但不限于，本文所述的启动子。在某些实施方式中，所述启动子是泛素-1启动子(例如，prUbi-10)。可将DNA构建体(例如，本文所述的表达盒和载体)直接引入植物组织，例如，采用射弹方法，例如DNA粒子轰击。
各crRNA和tracRNA，核酸酶可分开或作为一个表达载体的部分一起引入感兴趣的细胞(例如，玉米植物细胞)。在某些实施方式中，所述crRNA和tracRNA融合在一起，以产生导向核糖核酸(gRNA)。在一些实施方式中，所述gRNA包括(5’→3’)连接至tracRNA的crNA。在某些实施方式中，所述crRNA、tracRNA和核酸酶(例如，Cas9)以包含在一个表达载体中的核酸盒的形式一起引入。主题方法的各组分在下文中详细讨论。
导向RNA
在提供的一个方面中，本文提供包含CRISPR核糖核酸(crRNA)和反式作用RNA(tracRNA)的导向RNA(gRNA)。
主题gRNA的crRNA包含与PYR/PYL突变靶位点的序列互补的核苷酸序列，且包括基本相同于SEQ ID NO:363、364、365、366、367或369的序列。在某些实施方式中，所述crRNA具有基本相同于SEQ ID NO:363、364、365、366、367或369的序列。本文提供的主题crRNA特别可用于在PYR/PYL突变靶位点产生突变，其包括编码对应于PYL-E的V89(SEQ ID NO:363、364、365、366、367)和E149(SEQ ID NO:369)的氨基酸的核酸。本文所用的“PYR/PYL突变靶位点”指编码PYR/PYL受体的多核苷酸的区域，其包括其中通过主题方法引入了突变的位点。该crRNA与PYR/PYL突变靶位点以序列特异性方式，通过杂交至PYR/PYL突变靶位点中的序列(例如，PYR/PYL突变靶位点的互补链)来相互作用，并且，与该gRNA的tracRNA一起，招募Cas9内切核酸酶至PYR/PYL突变靶位点。通过该gRNA招募至PYR/PYL突变靶位点的Cas9内切核酸酶在PYR/PYL突变靶位点中引入双链断裂。本文所述的任何突变可发生在任何野生型PYR/PYL多肽中。类似的氨基酸取代可以，例如，通过比对待突变的PYR/PUL受体多肽序列和PYL-E受体多肽序列，在PYR/PYL受体而不是PYL-E中产生。PYR/PYL受体中的类似的氨基酸位置示于图2和3。
在一些实施方式中，所述PYR/PYL突变靶位点具有SEQ ID NO:362的序列，其包括编码PYL-E的V89的核酸。在一些实施方式中，所述PYR/PYL突变靶位点具有SEQ ID NO:368的序列，其包括编码PYL-E的E149的核酸。
在一些实施方式中，crRNA的长度是10个核苷酸(nt)-100个核苷酸(nt)。在一些实施方式中，所述crRNA的长度是15、16、17、18、19、20、21、22、23、24、25、26、27、28、29、30nt，并且包括基本相同于SEQ ID NO:362、363、364、365、366或368的序列。在一些实施方式中，所述crRNA的长度是至少17nt。在一些实施方式中，所述crRNA的长度是17nt-18nt、17nt-19nt、17nt-20nt、17nt-21nt、17nt-22nt、17nt-23nt、17nt-24nt、17nt-25nt、17nt-30nt、17nt-35nt、17nt-40nt、17nt-45nt、17nt-50nt、17nt-55nt、17nt-60nt、17nt-65nt、17nt-70nt、17nt-75nt、17nt-80nt、17nt-85nt、17nt-90nt、17nt-95nt，或17nt-100nt。在一些实施方式中，所述crRNA的长度是12nt-25nt、13nt-25nt、14nt-25nt、15nt-25nt、16nt-25nt、17nt-25nt、18nt-25nt、19nt-25nt、20nt-25nt、21nt-25nt，或22nt-25nt。
在一些实施方式中，所述crRNA长度是17nt，在一些实施方式中，所述crRNA长度是18nt。在一些实施方式中，所述crRNA长度是19nt。在一些实施方式中，所述crRNA长度是20nt。在一些实施方式中，所述crRNA长度是21nt。在一些实施方式中，所述crRNA长度是22nt。在一些实施方式中，所述crRNA长度是23nt。在一些实施方式中，所述crRNA长度是24nt。在一些实施方式中，所述crRNA长度是25nt。
在一些实施方式中，所述crRNA长度是至少17nt，在一些实施方式中，所述crRNA长度是至少18nt。在一些实施方式中，所述crRNA长度是至少19nt。在一些实施方式中，所述crRNA长度是至少20nt。在一些实施方式中，所述crRNA长度是至少21nt。在一些实施方式中，所述crRNA长度是至少22nt。在一些实施方式中，所述crRNA长度是至少23nt。在一些实施方式中，所述crRNA长度是至少24nt。在一些实施方式中，所述crRNA长度是至少25nt。
在一些实施方式中，所述导向RNA(gRNA)包括反式作用RNA(tracRNA)。所述主题导向RNA的反式作用RNA与crRNA相互作用以招募核酸酶至PYR/PYL突变靶位点的位置。在其招募至PYR/PYL突变靶位点之后，该核酸酶在该PYR/PYL突变靶位点中产生双链断裂(DSB)。可联合该主题gRNA采用能够招募Cas9至PYR/PYL突变靶位点的任何合适的tracRNA。在一些实施方式中，所述tracRNA由具有基本相同于SEQ ID NO:370的序列的核苷酸编码。在主题方法的某些实施方式中，将该tracRNA和crRNA分开引入植物细胞(例如，在不同表达载体上)。在一些实施方式中，该tracRNA连接至该crRNA并以导向RNA(gRNA)引入所述植物细胞。
在另一方面中，本文提供核酸，其包括编码任何本文所述的主题gRNA的多核苷酸。
在另一方面中，本文提供表达盒，其包括操作性地连接至任何本文所述的主题gRNA的RNA聚合酶启动子。可采用能够驱动编码主题gRNA的核酸的转录的任何合适的RNA聚合酶启动子。在一些实施方式中，所述启动子是诱导型启动子，包括但不限于本文所述的任何诱导型启动子。在其它实施方式中，所述启动子是组成型启动子，包括但不限于任何本文所述的组成型启动子。而在其它实施方式中，所述启动子是组织特异性启动子，包括但不限于，任何本文所述的组织特异性启动子。在某些实施方式中，所述RNA聚合酶启动子是RNA聚合酶III(polIII)启动子。在具体实施方式中，所述polIII启动子是U3启动子或U6启动子。在某些实施方式中，所述表达盒具有SEQ ID NO:371-373中任一项的序列。
包括gRNA和Cas9核酸酶的表达载体
在另一方面中，本文提供表达载体，其包括本文提供的一种或多种导向RNA(gRNA)表达盒，和包括操作性地连接至编码CRISPR相关联的内切核酸酶9(Cas9)的多核苷酸的启动子的表达盒。在一些实施方式中，操作性地连接至Cas9的启动子是泛素-1启动子(prUbi-10)。在一些实施方式中，所述表达包括包含编码具有基本相同于SEQ ID NO:363、364、365、366、367的crRNA的gRNA的多核苷酸的表达盒(参见例如，图5)。在一些实施方式中，所述表达盒载体包括包含编码具有基本相同于SEQ ID NO:369的crRNA的gRNA的多核苷酸的表达盒(参见例如，图6)。在一些实施方式中，所述表达载体包括第一表达盒，其包含编码具有基本相同于SEQ ID NO:363、364、365、366、367的crRNA的gRNA的多核苷酸；第二表达盒，其包含编码具有基本相同于SEQ ID NO:369的crRNA的gRNA的多核苷酸；和，第三表达盒，其包括操作性地连接至编码CRISPR相关联的内切核酸酶9(Cas9)的多核苷酸的启动子(参见例如，图7)。
采用RNA引导的基因组修饰产生PYR/PYL变体植物的方法
本文所述的表达载体可用于，例如，在植物中的基因组PYR/PYL突变靶位点处原位引入突变。因此，在另一方面中，本文提供产生在基因组PYR/PYL突变靶位点具有突变的植物的方法。在一些实施方式中，所述方法包括：将编码本文所述的gRNA和Cas9的表达载体和含有感兴趣的突变的至少一种修复核酸引入植物细胞。根据主题方法，所述crRNA和tracRNA将核酸酶引导至植物细胞基因组中的PYR/PYL突变靶位点。在其招募后，该核酸酶(例如，Cas9)在PYR/PYL突变靶位点处产生双链断裂。该PYR/PYL突变靶位点处的双链断裂促进包含感兴趣的突变的修复核酸与包括所述PYR/PYL突变靶位点的植物细胞基因组区域的同源重组，由此在PYR/PYL突变靶位点处引入所述突变。在某些实施方式中，所述修复核酸具有基本相同于SEQ ID NO:374-378任一项所示序列的序列。在其它实施方式中，该修复核酸具有与基本相同于SEQ ID NO:374-386的序列中任一项的序列互补的序列。在特定实施方式中，所述修复核酸具有基本相同于SEQ ID NO:376的序列。在其它实施方式中，该修复核酸具有与基本相同于SEQ ID NO:376的序列互补的序列。在另一个实施方式中，所述修复核酸具有基本相同于SEQ ID NO:378的序列。在其它实施方式中，该修复核酸具有与基本相同于SEQ ID NO:378的序列互补的序列。而在所述方法的另一实施方式中，引入两种修复核酸，其中所述修复核酸具有基本相同于SEQ ID NO:376和SEQ ID NO:378的序列。而在所述方法的另一实施方式中，引入两种修复核酸，其中所述修复核酸具有与基本相同于SEQID NO:376和SEQ ID NO:378的序列互补的序列。
在某些实施方式中，所述方法还包括选择具有所述突变的植物细胞的步骤。对于突变的选择可通过本领域已知的任何有用的技术进行，包括但不限于PCR扩增，然后测序，毛细管电泳和核酸酶Serveyer试验。在一些实施方式中，所述方法用于玉米植物的生产。
而在另一方面中，本文提供用于产生在本文所述的PYR/PYL核酸中具有突变的植物的试剂盒。在一些实施方式中，所述试剂盒包括任一本文所述的任何主题表达载体和至少一种修复核酸，其中，所述修复核酸包含PYL-E突变且能够通过Cas9切割事件之后的同源重组将PYL-E突变原位引入植物细胞基因组。在某些实施方式中，所述试剂盒包括修复核酸，其具有基本相同于SEQ ID NO:374-386的序列。
实施例
提供以下实施例用于说明而非限制本发明所要求的权利。
受体与靶配体的亲和性通常通过配体结合残基和所述配体之间的非共价相互作用来确定。所述残基中的突变对于受体–配体相互作用的强度可具有负向、正向或中性影响。受体-配体相互作用的亲和性固有地相关于引发生物效应所需的配体浓度，其中，相对于低亲和性配体，高亲和性配体所需浓度浓度较低。在一些情况中，当它们均在相同条件下通过相同浓度的活化配体活化时，相对于野生型受体，对于配体具有增加的亲和性的突变受体可引发更大生物效应。因此，使受体对于配体超敏的突变可用于工程改造相对于野生型对配体引发更强响应的生物体。此外，ABA超敏植物具有增强的ABA响应和改善的干旱耐受(Wang，Y.等Plant J.43，413–424(2005))。基于这些考虑，我们开始系统性地建立特定ABA受体突变，其增加ABA响应性。这通过测试配体结合残基中有所有可能的单一氨基酸取代突变PYR1变体的集合来实现。因此，我们对ABA-接触残基进行了位点-饱和的诱变，我们将它们定义为位于可及X射线坐标内内或ABA或ABA-接触水残基的那些。先前构建该突变体集合，描述于PCT申请号PCT/US2012/043121和Mosquna等，Proc Natl Acad Sci USA108:20838–20843(2011)。该突变体集合通过诱变先前描述的pBD GAL-PYR1模板来制备(Park，S.-Y.等Science 324，1068–1071(2011))。响应ABA，该具体的质粒编码融合蛋白，其结合至共表达的GAL4活化结构域-HAB1融合蛋白(由质粒pACT-HAB1编码)。该结合重建功能性GAL4转录活化物，以及β-半乳糖苷酶报告基因的后续转录，其进而允许在裂解的细胞暴露至底物X-gal时对激动剂促进的受体-PP2C相互作用进行基于比色的检测。将突变克隆分别转化进入包含pACT-HAB1的酿酒酵母(S.cerevisiae)株Y190。根据缺乏Leu和Trp的合成右旋糖(SD)琼脂板(SD–LT)上质粒的存在来选择酵母转化体，并通过采用X-gal染色以监测β-gal报告基因表达水平来检测PP2C相互作用。将个体克隆排列进入96孔板，然后点样至包含0、0.5或5.0μM(+)-ABA的SD–LT平(即单孔)板上。各试验板包含95个突变克隆和一个野生型PYR1阳性对照克隆。点样的细胞在30℃培养48小时，之后它们通过氯仿裂解并用X-gal溶液染色，如先前描述(Park，S.-Y.等(2009)Science 324，1068–1071)。阳性定义为在0.5μM(+)-ABA上显示染色但在缺乏(+)-ABA的板上无染色的那些突变体。在该初始筛选过程后，全部阳性克隆在包含0、0.25、0.5和1μM(+)-ABA的板上重新测试，并经染色测试半乳糖苷酶活性，如上所述。在0.5μM(+)-ABA或更低上显示可检测染色的突变克隆计为超敏突变。图1描述PYR1突变体-HAB1相互作用的结果，在不同ABA浓度下的酵母双杂交试验中测试，其中较深斑点指示增加的相互作用。该数据也汇总如下：
通过组合诱变构建的高度超敏ABA受体
单个鉴定的单超敏突变之间的加和或协同相互作用可增加受体对于ABA的敏感性。为了鉴定可能有利的组合，我们采用组合诱变来构建包含鉴定的最佳单突变体的亚组的组合的受体，然后筛选它们以鉴定具有增加的敏感性的受体。设计与PYR1编码序列的合适区域互补的诱变引物，以允许将以下突变纳入PYR1模板DNA：F61L、F61M、V81I、V81Y、I110C、I110S、E141I、E141L、E141M、E141Y、A160C、A160I、A160V。将等摩尔浓度的这些引物与针对各靶位点的野生型引物的混合物合并(4mol百分比，相对于突变引物池)，然后该引物混合物用于QuickChange Lightning多重定点PCR诱变试剂盒(安捷伦(Agilent)，美国)，采用pBD-PYR1模板DNA。用于反应混合物中的野生型引物的应用原则上允许全部二重、三重、四重和五重突变体组合在诱变反应中合成。将反应产物转化进入感受态大肠杆菌细胞以产生约10,000个克隆的池，其随后用以制备突变文库的质粒DNA。后续将突变质粒的池引入先前描述的pAD-HAB1MAV99报告株(Peterson，F.C.等(2010)Nature Structural&Molecular Biology 17，1109–1113)。在该报告株中，GAL4启动子驱动URA3报告基因在遗传背景中的表达，其中该内源URA3基因被破坏，这允许采用尿嘧啶缺陷型培养基进行正选择。因此，编码可与HAB1相互作用的受体的突变克隆可采用该系统正选择。然后，将包含该突变受体文库的转化酵母细胞铺板至缺乏尿嘧啶且包含50nM ABA的生长培养基，该ABA浓度过低以不允许对照株生长。鉴定了具有尿嘧啶独立型生长的26个集落，其经分离并在缺乏ABA的培养基上重新测试，以去除允许受体与HAB1的配体独立型(即组成型)相互作用的克隆。来自包含非组成型受体的酵母细胞的质粒经分离和测序，其揭示如下4种已经分离的高度超敏组合突变体：
PYR1F61L、A160C，
PYR1F61M、A160V，
PYR1F61M、I110S、A160V，
PYR1F61L、V81I、I110C、A160V。
将这些突变体各自及其对应的单一突变的质粒转化进入先前描述的酵母报告株，Y190pAD-HAB1(Park，S.-Y.等(2009)Science 324，1068–1071)。使转化的酵母细胞在包含一定范围的ABA浓度的选择性培养基上生长，然后细胞经裂解并染色以揭示β-半乳糖苷酶活性，示于图4。
突变超敏受体的增加的亲和性
PYR1和HAB1酵母双杂交株的配体敏感性一般与受体亲和性相关联。为了检测通过我们的功能筛选鉴定的超敏突变是否是该情况，我们进行了等温滴定量热法(ITC)，以检测通过突变受体-ABA结合反应产生的热，并推断配体结合解离常数(Kd)。野生型PYR1的亲和性已在先前采用ITC测量，且估计是97±36μM(Dupeux等2010)。PYR1-A160V突变受体在大肠杆菌BL21(DE3)中以融合至小泛素样蛋白质SUMO的形式表达，采用载体pSUMO(LifeSensors，美国)，其改善了蛋白质在大肠杆菌中的溶解性且包含促进采用固定的金属亲和性色谱(IMAC)的纯化的NH2-末端六组氨酸标签。采用PCR产物(产生自作为模板的pBD-PYR1(A160V)酵母双杂交构建体)将PYR1A160V克隆进入pSUMO，并验证序列。将短柔性接头和烟草蚀纹病毒(TEV)蛋白酶切割位点(序列NH2-GGGSQFGSGGGGGSGSENLYFQS-COOH；SEQ IDNO:411)纳入SUMO标签和受体之间，以允许重组蛋白质通过TEV蛋白酶的切割，其产出PYR1(A160V)加NH2-末端QS附加物。重组SUMO-TEV-PYR1(A160V)蛋白质在大肠杆菌中生成，并通过固定金属亲和性色谱纯化，如先前描述(Okamoto等，Proceedings of the NationalAcademy of Sciences of the United States of America110，第29期(2013):12132–12137)。纯化的融合蛋白根据已建立的方案用重组TEV蛋白酶消化，然后通过将切割反应物通过IMAC柱,将切割的蛋白质与SUMO标签和未切割的蛋白质分离，该柱不保留切割的PYR1(A160V)产物。切割的蛋白质采用Superdex柱(美国通用电气医疗集团)通过凝胶过滤纯化，并采用Amicon滤器(EMD，USA)通过离心浓集法浓缩，如先前描述(Dupeux等，The EMBOJournal 30，No.20(2011):4171–4184)。浓缩蛋白质用于ITC实验，采用TA仪器Nano ITCLow Volume仪，每300秒重复注射2.5μL的600μM(+)-ABA溶液进入包含60μM PYR1(A160V)的反应细胞，持续200分钟。ABA和蛋白质溶解于包含PBS、1mM 2-巯基乙醇和0.012％DMSO的缓冲液。产生的热图采用该仪器配套软件处理，以标准化拟合单一结合位点模型，其产生1.5μM的Kd和1.068的结合化学计量。这些数据显示，相对于野生型PYR1，A160V突变具有增加的ABA亲和性，这与通过酵母双杂交试验指示的增加的敏感性相一致。
靶向的基因组修饰
含有特定基因中诱导的突变的非转基因植物可以多种方式获得。生物体的化学诱变可用以产生随机基因组范围的突变，并且诱变的个体的群可采用高通量突变检测方法扫描以鉴定感兴趣的基因中含有特异性突变的个体。例如，TILLING(定向诱导基因组局部灶点)允许研究者鉴定在基因中非天然产生的诱导的突变，通过采用PCR来从数千诱变的个体扩增感兴趣的基因，并利用异源双链核酸分子特异性的核酸酶，例如芹菜核酸酶CEL1，来鉴定在PCR扩增的区域中含有突变的植物(McCallum，C.M.等(2000).Nat.Biotechnol.18,455–457)。除了内切核酸酶以外，许多技术可用于多态性鉴定，包括对获自诱变的个体的PCR产物的直接测序。
为了鉴定包含具有增加的敏感性的ABA受体的玉米植物，产生了EMS诱变的群，并且，该群中所有ABA受体基因从数千诱变的植物经PCR扩增。采用TILLING法扫描扩增的产物的多态性，并对鉴定的多态性片段测序以确定特异性突变存在。由此，鉴定了含有对应于本申请所述的多态性的突变的个体。
采用该策略获得的最可能的突变体是可通过单核苷酸取代经编码的那些，其可通过查阅密码子表来建立。例如，通过基于该基因序列筛选不同单核苷酸取代，例如UUU→CUU，或UUC→CUC，可在受体同系物中获得带有同源于PYR1中F61L或F61M的突变的受体。对于A160V(GCN→GUN)、V81I(GUU→AUU、GUC→AUC、GUA→AUA)、V81Y(GUU→UUU、GUC→UUC)也是如此。原则上，任何单一突变可通过化学诱变TILLING分离，但在实践中，最有可能获得可能通过单核苷酸取代产生的变化的亚组。提供上述的实例是典型且非详尽的，并且，允许所需突变，例如E141V和I110S，的其它单核苷酸取代也是可行的。
可采用其它突变诱导系统来靶向特定基因中的突变，例如基因组编辑方法，其具有在指定靶位点处增加单一和多重突变的频率的益处(Lozano-Juste,J.和Cutler,S.R.(2014)Trends in Plant Science 19，284–287)。双链DNA断裂(DSB)在基因组中的序列特异性引入导致将DNA修复因子招募至断裂位点处，它们随后通过易错非同源末端接合(NHEJ)或同源重组(HR)途径修复灶点(lesion)。NHEJ修复断裂，但不精确且常在DSB处或周围产生不同的突变。在其中HR机构修复DSB的细胞中，可将具有侧接DSB的同源的序列，包括外源提供的序列，纳入DSB的区域处。因此，DSB可被遗传学家利用以增加确定位点处的突变频率，然而HR和NHEJ的相对作用之间的固有差异可影响靶标基因座处的突变类型。已开发多种技术以在特异性位点产生DSB，包括合成锌指核酸酶(ZFN)、转录活化物样内切核酸酶(TALEN)和最近的规律成簇的间隔短回文重复(CRISPR)/CRISPR相关联的蛋白质9(Cas9)系统。该系统基于抵抗侵入性细菌噬菌体的细菌免疫系统，其中，2个小RNA，CRISPR-RNA(crRNA)和反式作用crRNA(tracrRNA)的复合物引导核酸酶(Cas9)至与crRNA互补的特定DNA序列。采用任何这些系统，研究者可在在表达基因组编辑构建体的细胞中的预定位点处产生DSB。为了产生同源重组，必须提供同源于靶向位点的DNA盒，优选在高浓度，从而有利于HR，或NHEJ。可考虑多种策略用于实现该目的，包括采用土壤杆菌介导的转化或DNA模板的粒子轰击的模板递送，以及近期描述的利用修饰的病毒基因组来提供双链的DNA模板的方法。例如，Baltes等2014(Baltes，N.J.等(2014)Plant Cell 26，151–163)近期显示采用土壤杆菌介导的转化被引入植物细胞的工程改造的双粒病毒组，其可经工程改造以在其中共表达ZFN的细胞中产生DNA重组模板。
在一些方面中，一旦DSB已采用任何数量的技术被产生，可通过核苷酸取代、缺失或插入的同源重组，利用此类位点来促进靶向的遗传变化的分离。例如，ABA受体基因可用基因组编辑技术靶向，并且诱变的植物的后裔植物可采用上文概述的方法筛选，以鉴定增加ABA敏感性的位点处的突变。基因组编辑构建体向生物体中的递送可涉及不稳定瞬时表达构建体或构建体向基因组中的稳定整合，通过土壤杆菌介导的转化递送。在后一种情况中，稳定的转基因植物可用于在植物中表达基因组-编辑构建体以增加靶位点处的突变频率。一旦所需突变体通过多态性扫描(类似于TILLING中所用那些)分离，个体可以是回交的野生型系，以隔离开转基因插入事件。
概念上，这些方法类似于TILLING，并且，用于鉴定确定的突变的方法是相似的，然而，鉴于这些方法的靶向性质，确定位点处的突变频率将更高，且涉及多于单一碱基对的变化的突变可更容易地被鉴定。
由CRISPR-Cas9介导的玉米ABA受体的靶向的氨基酸修饰
1.玉米ABA受体(ZmPYL)靶基因修饰
植物ABA受体的体内修饰例如采用玉米ZmPYL基因来描述。玉米基因组包含多于10个ABA受体(ZmPYL-A至ZmPYL-P)，其与AtPYL1共有若干高度保守氨基酸。为了获得内源ZmPYL-E(GRMZM2G165567)基因中的ABA超敏突变，分别选择两个氨基酸(V89和E149)作为序列特异性诱变成所需突变氨基酸(丙氨酸和亮氨酸(V89A和E149L))的靶标，采用由序列特异性核酸酶介导的同源导向的基因修复和供体DNA模板。目前，存在用于制造序列特异性核酸酶的若干技术平台，包括例如锌指核酸酶、TALE核酸酶、CRISPR-Cas9和工程改造的大范围核酸酶(Puchta和Fauser(2014)，用于植物中基因组工程改造的合成核酸酶：光明未来前景(Synthetic nucleases for genome engineering in plants:prospects for abright future).Plant Journal 78:727–741；Chen和Gao(2014)靶向基因组修饰技术机器在作物改进中的应用(Targeted genome modification technologies and theirapplications in crop improvements).Plant Cell Rep.33:575–583)，它们中的任一个均可用于产生在本文所述的基因组编码序列PYR/PYL多肽中含有原位超敏突变的植物。
如下实施例描述了CRISPR-Cas9系统用于在内源ZmPYL-E基因中产生靶向的基因修饰的应用。CRISPR-Cas9介导的基因修饰需要这些组分：Cas9核酸酶、识别诱变靶标的crRNA(CRISPR RNA)，tracRNA(反式作用RNA)和修复供体DNA模板分子。出于使用便利性的考虑，crRNA和tracRNA经融合并作为单一导向RNA分子(gRNA或sgRNA)递送[Sander和Joung(2014)用于编辑、调节和靶向基因组的CRISPR-Cas系统(CRISPR-Cas systems forediting,regulating and targeting genomes)32:347-355]。
2.Cas9及其在玉米细胞中表达的优化
为了实现玉米细胞中的良好表达，来自酿脓链球菌(Streptococcus pyogenes)SF370的II型Cas9基因用玉米-优选的密码子优化。核定位信号也被纳入Cas9的C末端以改善其对核的靶向。以下是优化的Cas9序列(cBCas9Nu-01，aka，cCas9-01)。为了在玉米细胞中表达Cas9，玉米优化的Cas9基因(cBCas9Nu-01)受玉米泛素-1启动子(prUbi1-10)的控制，随后是终止子序列(tNOS)(图5)。
3.gRNA设计和表达
3.1用于介导V89A修饰的gRNA：结构及其表达
对于靶向的V89A修饰，针对玉米ZmPYL-E基因组靶序列(5’-CGCGA CGTCA ACGTCAAGAC-3’)(SEQ ID NO:362)设计至少17核苷酸(nt)长的crRNA，其后是5’-CGG-3’PAM(前间区序列邻近基序)序列，用于Cas9介导的靶标识别。例如，17-nt(5’-GA CGUCA ACGUCAAGAC-3’)(SEQ ID NO:363)，18-nt(5’-CGA CGUCA ACGUC AAGAC-3’)(SEQ ID NO:364)，19-nt(5’-GCGA CGUCA ACGUC AAGAC-3’)(SEQ ID NO:365)，20-nt(5’-CGCGA CGUCA ACGUCAAGAC-3’)(SEQ ID NO:366)或21-nt(5’-G CGCGA CGUCA ACGUC AAGAC-3’)(SEQ ID NO:367)的gRNA可用于导向ZmPYL-E靶标的Cas9切割。crRNA与tracRNA和Cas9蛋白或mRNA共递送以介导靶位点切割。优选地，crRNA分子与tracRNA分子共价融合成单一导向RNA(gRNA)。gRNA可化学合成或通过体外转录产生。如果修复供体寡核苷酸是共递送的，则体外产生的gRNA可直接用于物理递送，例如，采用Cas9RNA或蛋白质的生物弹射轰击，以介导靶标切割和同源导向的靶标修饰。更优选地，gRNA在植物中从含有RNA聚合酶III(PolIII)启动子例如植物U3和U6启动子例如稻U3和U6启动子(prOsU3和prOsU6)的DNA表达盒产生。对于prOsU3，转录起始以核苷酸A开始，而对于prOsU6，转录起始以核苷酸G开始(Shan等(2013)Nature Biotechnology31:686-688；Xie和Yang(2013)Molecular Plant 6:1975-1983)。例如，为了产生靶向内源ZmPYKL-E序列(5’-CGCGA CGTCA ACGTC AAGAC-3’)(SEQ ID NO:362)的gRNA，将19-nt DNA寡核苷酸(5’-GCGA CGTCA ACGTC AAGAC-3’)(SEQ ID NO:365)或21-nt寡核苷酸(5’-G CGCGA CGTCA ACGTC AAGAC-3’)(SEQ ID NO:367)融合至编码tracRNA支架((5’-gtttt agagctagaa atagc aagtt aaaat aaggctagtc cgtta tcaac ttgaa aaagtggcac cgagt cggtg c-3’)(SEQ ID NO:370)和PolIII终止序列(5’-gtttt agagctagaaatagc aagtt aaaat aaggctagtc cgtta tcaac ttgaa aaagt ggcac cgagt cggtg cttttttttt-3’，Mali等(2013)Science 339:823-826)的DNA序列，并置于稻聚合酶III启动子U3(prOsU3)或U6(prOsU6)的控制下。以下是含有prOsU3和含有与tracRNA融合的19-nt V89AcrRNA(下划线)的gRNA的编码序列的表达盒的序列(SEQ ID NO:371)。该序列伴随Cas9表达盒被克隆进入生物弹射转化载体，以形成载体pZmPYLE-V89A(图5)。
下方序列实例(SEQ ID NO:372)描述含有prOsU6启动子和含有21-nt V89A crRNA(下划线)和tracRNA的gRNA编码序列的表达盒。
3.2用于介导E149L修饰的gRNA：结构及其表达
对于玉米ZmPYL-E的靶向的E149L修饰，玉米基因组靶序列(5’-GCACC CTGGTGATCG AGTCG TTCGT GGTCG-3’)(SEQ ID NO:368)中两个带下划线的碱基需要被转化成CT，以形成突变序列(5’-GCACC CTGGT GATCC TGTCG TTCGT GGTCG-3’)。为此，用于编码20-nt导向RNA(5’-CCTGG TGATC CTGTC GTTCG-3’，xZmPYLE-E149L)(SEQ ID NO:369)、tracRNA支架和PolIII终止序列(5’-gtttt agagctagaa atagc aagtt aaaat aaggctagtc cgttatcaac ttgaa aaagt ggcac cgagt cggtg ctttt ttttt-3’，Mali等(2013)Science 339:823-826)的序列的表达盒置于稻聚合酶III启动子U6(prOsU6)的控制下，示于图6。prOsU6启动子在核苷酸G之后起始转录。在图6中，prOsU6-E149L gRNA表达盒具有如下序列(SEQID NO:373)，其中20bp靶向导向序列(xZmPYLE-E149L或xZmPYLe，SEQ ID NO:369)带下划线。
或者，导向RNA可还从不同聚合酶III启动子如稻U3启动子(prOsU3)表达，其在核苷酸A之后起始转录。prOsU3-E149L gRNA表达盒具有如下序列(SEQ ID NO:374)，其中20bp靶向导向序列(xZmPYLE-E149L或xZmPYLe，SEQ ID NO:369)带下划线。将该prOsU3-E149LgRNA表达盒连同PMI选择性标志物基因盒和prSoUbi4驱动Cas9基因表达盒插入二元载体骨架以形成转化载体23190(图8)。
4.具有ZmPYL-E基因中靶向的基因组序列修饰的突变体的生成
4.1采用生物弹射轰击的ZmPYL-E基因中靶向的突变V89A的生成
对于由同源导向的修复介导的靶基因序列修饰，供体DNA分子需要与Cas9和gRNA共递送。具有侧接Cas9切割位点的至少15个核苷酸且包含意图的突变核苷酸的DNA分子用作修复供体。对于产生V89A突变的靶序列5’-CGCGA CGTCA ACGTC AA/GAC-3’的修饰，单一带下划线的残基T需要转化成C，所以位于位置89的缬氨酸(V89，GTC)改变为丙氨酸(A89，GCC)。鉴于意图的Cas9切割位点(由/指示)是下游9个核苷酸处，优选地，修复DNA分子应包含TCA上游至少15-nt和AA/GAC中的带下划线的A的下游15-nt的序列，如在该序列中(5’-GCAGCCT GCGCGACGCC AACGTCAA/GA CCGGCCTGCC GGC-3’)(SEQ ID NO:375)。更优选地，修复DNA分子应包含TCA上游至少20-nt和AA/GAC中带下划线的A下游至少20-nt的序列，如该序列中概述(5’-GG TCGGCAGCCT GCGCGACGCC AACGTCAA/GA CCGGCCTGCC GGCGACGA-3’)(SEQ ID NO:376)。更优选，修复DNA分子应包含TCA上游多于30-nt和AA/GAC中带下划线的A下游多于30-nt的序列，如该序列中概述(5’-AC CAGCTC GAGG TCGGCAGCCT GCGCGACGCCAACGTCAA/GA CCGGCCTGCC GGCGACGACC AGAACCGA-3’)(SEQ ID NO:377)。最优选地，修复DNA分子应包含TCA上游多于50-nt和AA/GAC中带下划线的A下游多于50-nt的序列，如该序列中所示(5’-GA ACTGCGTCGT GCGCGGGGAC CAGCTC GAGG TCGGCAGCCT GCGCGACGCCAACGTCAA/GA CCGGCCTGCC GGCGACGACC AGAACCGAGC GCCTCGAGCA GCTCGACGA-3’)(SEQ IDNO:378)。应注意，具有对应于SEQ ID NO:375至SEQ ID NO:378的相反链的序列的寡核苷酸也可用于介导靶向的V89A突变。
为产生携带V89A突变的植物，包含TCA上游30-nt和AA/GAC中带下划线的A下游30-nt的序列的上述的修复供体DNA寡核苷酸(5’-AC CAGCTC GAGG TCGGCAGCCT GCGCGACGCCAACGTCAA/GA CCGGCCTGCC GGCGACGACC AGAACCGA-3’)(SEQ ID NO:377)与pZmPYLE-V89A载体(图1)共沉淀至金粒子上，并轰击进入未成熟玉米胚(基因型A188，HiII或其它可用变体)。用于玉米未成熟胚轰击方法、愈伤组织诱导组织再生方法和生根方法已于先前描述，除了本文不需甘露糖选择(Wright等，2001，采用磷甘露糖异构酶基因pmi作为可选择标志物的玉米(Zea mays L.)和小麦(Triticum aestivum L.)的有效生物弹射转化(Efficientbiolistic transformation of maize(Zea mays L.)and wheat(Triticum aestivum L.)using the phosphomannose isomerase gene,pmi,as the selectable marker).PlantCell Reports 20:429–436.)。简言之，未成熟胚在授粉后约9-12天从收获的未成熟穗分离，并在渗压剂培养基上预培养3-5天。然后用DNA载体ZmPYLE-V89A和供体寡核苷酸，采用BioRad PDS-1000生物弹射粒子递送系统，轰击预培养胚。然后，使被轰击的胚在愈伤组织诱导培养基中孵育，然后移至再生培养基上以诱导成芽。然后将芽移至生根培养基。优选但非必需地，还向ZmPYLE-V89A载体添加可选择标志物基因盒如PMI，所以仅包含完整的gRNA或Cas9表达盒的转化细胞将被选择用于再生。然后从再生的植物收获样品，用于基因分型以鉴定在ZmPYL-E基因中包含所需V89A突变的植物。基因分型可采用一种或多种标准突变检测方法例如PCR扩增然后测序、毛细管电泳和核酸酶Surveyer试验来进行。
也可采用其它物理递送方法例如原生质体转化和碳化硅晶须介导的转化，将携带gRNA和Cas9表达盒的ZmPYLE-V89A载体递送进入玉米细胞。修复供体DNA分子还可以作为重组DNA分子的部分例如限制性片段或质粒或T-DNA或病毒复制子的单链或双链分子的形式递送进入细胞，用于采用本领域已知的方法生成转化细胞。或者，可采用土壤杆菌介导的转化将gRNA和Cas9表达载体和修复供体载体转化进入玉米细胞。应注意，对于靶向修饰，无Cas9或gRNA表达载体的整合是需要或甚至优选的。因此，这些载体可通过生物弹射转化或土壤杆菌介导的转化瞬时递送。
4.2采用生物弹射和土壤杆菌介导的转化的ZmPYL-E基因中靶向的突变E149L的生成
类似于用于产生ZmPYLE-V89A突变的上述实施例(部分4.1)，采用生物弹射轰击，采用携带Cas9和gRNA表达盒的DNA载体(例如示于图6和图8的那些)连同包含所需突变的修复供体DNA序列，例如具有该序列的纯化的寡核苷酸形式(ZmPYL-Eb，SEQ ID NO:379，5’-TGACG GGAGG CCGGG CACCC TGGTG ATCCT GTCGT TCGTA GTCGA TGTGC CTGAT GGCAA-3’，表2)，可将靶向的E149L突变(表1)引入ZmPYL-E基因。也可采用其它形式的修复供体寡核苷酸。例如，寡核苷酸可以更长或在互补链中或包含化学修饰(例如硫代磷酸酯或甲基膦酸酯)以增强稳定性或对靶序列的亲和性。化学修饰的寡核苷酸已经描述(Deleavey和Damha，2012，Chemistry&Biology，http://dx.doi.org/10.1016/j.chembiol.2012.07.011)。为了显示所述化学修饰的寡核苷酸的应用，采用具有来自非编码链的序列且还包含硫代磷酸酯连接的寡核苷酸(表2，ZmPYL-Ec-NT-PM，SEQ ID NO:380，5’-T*T*C*GT GTTGC CATCA GGCACATCGA CTACG AACGA CAGGA TCACC AGGGT GCCCG GCCTC CCGTC AATG*C*T*C-3’，*表示核苷酸之间存在硫代磷酸酯连接)来进行实验。
ZmPYL-E基因中的靶向的突变E149L还可采用通过土壤杆菌递送的DNA分子产生。土壤杆菌介导的转化方法已经在他处描述(Ishida等(1996).由根癌土壤杆菌介导的玉米(Zea mays L.)高效转化(High efficiency transformation of maize(Zea mays L.)mediated by Agrobacterium tumefaciens).Nat.Biotechnol.14,745–750；Negrotto等(2000).磷酸甘露糖-异构酶作为通过土壤杆菌转化恢复转基因玉米植物(Zea mays L.)的可选择标志物的磷酸甘露糖-异构酶的应用(Theuse of phosphomannose-isomerase as aselectable marker to recover transgenic maize plants(Zea mays L.)viaAgrobacterium transformation)Plant Cell Rep.19,798–803)。简言之，prOsU6-E149L(SEQ ID NO:373)或prOsU3-E148L(SEQ ID NO:374)gRNA表达盒被克隆进入携带PMI可选择标志物盒和具有玉米优选的密码子Cas9的表达盒的二元载体，形成转化载体pZmPYLE-E149L和23190(图6、图8、表1)。采用土壤杆菌介导的转化，这些载体可用以递送Cas9和gRNA表达盒进入玉米细胞。包含意图的突变序列(5’-TGACG GGAGG CCGGG CACCC TGGTG ATCCTGTCGT TCGTA GTCGA TGTGC CTGAT GGCAA-3’)(SEQ ID NO:379)的修复供体DNA分子从分开的T-DNA分子被共递送进入细胞。然而，其还可被插入二元载体pZmPYLE-E149L或23190中的gRNA和Cas9表达盒旁边的T-DNA区域(图2)。供体修复模板还可以源自另一T-DNA的病毒复制子形式递送(Baltes等2014，用于植物基因组工程改造的DNA复制子(DNA replicons forplant genome engineering).Plant Cell.26:151-163)。PMI标志物用以选择具有完整的Cas9或gRNA表达盒的转基因植物。然而，应注意，转化载体的稳定转化并非必需或甚至优选以产生所需突变，只要筛选足够的植物即可，因为Cas9和gRNA的瞬时表达足以导致染色体靶序列的切割以诱导DNA修复。然而，应注意，对于靶向的修饰，无Cas9或gRNA表达载体的整合是必需或甚至优选的。
4.3ZmPYL-E基因中多重氨基酸修饰的同时生成
应注意，如果用于多重靶序列的gRNA和修复供体同时存在，则可同时修饰多于一个靶标。例如，V89A和E149L突变可同时通过共轰击包含Cas9和两种gRNA的表达盒的载体pZmPYLE-V89A-E149L(图7)，连同两种修复供体DNA模板，V89A寡核苷酸(5’-AC CAGCTCGAGG TCGGCAGCCT GCGCGACGCC AACGTCAA/GA CCGGCCTGCC GGCGACGACC AGAACCGA-3’)(SEQID NO:377)和E149L寡核苷酸ODN-ZmPYL-Eb(5’-TGACG GGAGG CCGGG CACCC TGGTG ATCCTGTCGT TCGTA GTCGA TGTGC CTGAT GGCAA-3’)(SEQ ID NO:379)或ODN-ZmPYL-Ec-NT-PM(SEQ ID NO:380，5’-T*T*C*GT GTTGC CATCA GGCAC ATCGA CTACG AACGA CAGGA TCACCAGGGT GCCCG GCCTC CCGTC AATG*C*T*C-3’，*表示存在核苷酸之间的硫代磷酸酯连接)来获得。
如同关于产生具有单一突变的植物所述，被轰击的胚然后在愈伤组织诱导培养基中孵育，随后移至再生培养基上以诱导成芽。然后将芽移至生根培养基。PMI标志物可用以选择具有完整的Cas9或gRNA表达盒的转基因植物。然而，应再次注意，转化载体的稳定转化并非必需或甚至优选以产生所需突变，只要筛选足够的植物以鉴定具有所需突变的植物即可，因为Cas9和gRNA的瞬时表达足以导致染色体靶序列的切割以诱导DNA修复。然后从再生的植物收获样品，用于基因分型以鉴定在ZmPYL-E基因中包含所需V89A和E149L突变的植物。基因分型可采用一种或多种标准突变检测方法例如PCR扩增然后测序、毛细管电泳和核酸酶Surveyer试验来进行。
4.4在ZmPYL-E基因中的V89位置的其它靶向的突变的生成
可在ZmPYL-E的V89位置引入替代定点变化以获得ABA超敏突变，通过采用与上文关于产生V89A突变所述的方法相似的方法，不同之处在于修复供体寡核苷酸序列需经改变以引入对应突变。例如，可通过采用相同gRNA(SEQ ID NO:363-367)以引导ZmPYL-E靶标的Cas9切割，来引入V89I和V89Y突变。通过任何物理或生物方法例如生物弹射轰击或土壤杆菌介导的转化，可将gRNA和Cas9的表达盒同时递送进入玉米细胞。对于V89I突变的引入，ZmPYL-E基因组靶序列5’-CGCGA CGTCA ACGTC AA/GAC-3’中的单一带下划线的残基G需转化成A，从而位于位置89处的缬氨酸(V89，GTC)改变为异亮氨酸(I89，ATC)。鉴于意图的Cas9切割位点(由/指示)是下游9个核苷酸处，优选地，修复DNA分子应包含GTC上游至少15-nt和AA/GAC中带下划线的A下游15-nt的序列，如该序列所示(5’-GGCAGCCT GCGCGACATCAACGTCAAGA CCGGCCTGCC GGC-3’)(SEQ ID NO:381)。更优选，修复DNA分子应包含ATC上游至少20-nt和AAGAC中带下划线的A下游至少20-nt的序列，如该序列中概述(5’-AGGTCGGCAGCCT GCGCGACATC AACGTCAAGA CCGGCCTGCC GGCGACGA-3’)(SEQ ID NO:382)。更优选地，修复DNA分子应包含ATC上游多于30-nt和AAGAC中带下划线的A下游多于30-nt的序列，如该序列中概述(5’-GAC CAGCTC GAGG TCGGCAGCCT GCGCGACATC AACGTCAAGACCGGCCTGCC GGCGACGACC AGAACCGA-3’)(SEQ ID NO:383)。最优选地，修复DNA分子应包含ATC上游多于50-nt和AAGAC中带下划线的A下游多于50-nt的序列，如该序列中所示(5’-GGAACTGCGTCGT GCGCGGGGAC CAGCTC GAGG TCGGCAGCCT GCGCGACATC AACGTCAAGA CCGGCCTGCCGGCGACGACC AGAACCGAGC GCCTCGAGCA GCTCGACGA-3’)(SEQ ID NO:384)。
对于V89Y突变的引入，玉米基因组靶序列5’-CGCGA CGTCA ACGTC AA/GAC-3’中两个带下划线的残基GT需要转化成TA，从而位于位置89的缬氨酸残基(V89，GTC)改变为酪氨酸(Y89，TAC)。鉴于意图的Cas9切割位点(由/指示)是下游9个核苷酸处，优选地，修复DNA分子应包含GTC上游至少15-nt和AA/GAC中带下划线的A下游15-nt的序列，如该序列中所示(5’-GGCAGCCT GCGCGACTAC AACGTCAAGA CCGGCCTGCC GGC-3’)(SEQ ID NO:385)。更优选地，修复DNA分子应包含TAC上游至少20-nt和AAGAC中带下划线的A下游至少20-nt的序列，如该序列中概述(5’-AGG TCGGCAGCCT GCGCGACTAC AACGTCAAGA CCGGCCTGCC GGCGACGA-3’)(SEQ ID NO:386)。更优选地，修复DNA分子应包含TAC上游多于30-nt和AAGAC中带下划线的A下游多于30-nt的序列，如该序列中概述(5’-GAC CAGCTC GAGG TCGGCAGCCTGCGCGACTAC AACGTCAAGA CCGGCCTGCC GGCGACGACC AGAACCGA-3’)(SEQ ID NO:387)。最优选地，修复DNA分子应包含TAC上游多于50-nt和AA/GAC中带下划线的A下游多于50-nt的序列，如该序列中所示(5’-GGA ACTGCGTCGT GCGCGGGGAC CAGCTC GAGG TCGGCAGCCTGCGCGACTAC AACGTCAAGA CCGGCCTGCC GGCGACGACC AGAACCGAGC GCCTCGAGCA GCTCGACGA-3’)(SEQ ID NO:388)。
类似地，包含V89I(或V89Y)和E149L的双突变体(参见例如，SEQ ID NO:390、391和392)可通过用包含连同寡核苷酸的Cas9和两个gRNA的表达盒的载体转化玉米细胞来获得，以引入对应突变(E149L、V89I或V89Y)，如上文部分4.3中所述。
5.其它ZmPYL基因家族成员ZmPYL-D、ZmPYL-F和ZmPYL-J中的靶向的E169L基因组序列修饰的生成
5.1诱变靶标和gRNA设计
与上文关于内源ZmPYL-E基因所述实施例(实施例4和表1)相似，还选择其它ZmPYL基因家族成员用于靶标的基因组编辑，以替代特定核苷酸，从而对应于ABA受体中的E169的氨基酸残基(ZmPYL-D、ZmPYL-F和ZmPYL-J)改变为超敏形式L169。这些意图的变化汇总于表1。这些实验意在将对应转化的氨基酸残基E(谷氨酸)修饰成同源ZmPYL基因中的L(亮氨酸)。
表1 ZmPYL突变和gRNA序列和转化载体
5.2用于表达靶向ZmPYL-D、ZmPYL-F和ZmPYL-J基因的gRNA的载体构建
与上文关于用于表达内源ZmPYL-E基因的gRNA的构建体23190所述的实施例(实施例4)相似，构建表达Cas9和ZmPYL-D(23136和23189)、ZmPYL-F(22981和23191)和ZmPYL-J(23192)基因的不同gRNA(表1)的转化载体(图9A-9B、10A-10B和11)。不同ZmPYL基因的gRNA靶向序列列于表1(SEQ ID NO:393-397)。在这些载体中，完整gRNA编码区域[约20个核苷酸靶向导向(SEQ ID NO:393-397)，tracRNA支架和PolIII终止序列(5’-gtttt agagctagaaatagc aagtt aaaat aaggctagtc cgtta tcaac ttgaa aaagt ggcac cgagt cggtg cttttttttt-3’)]受稻聚合酶III U3启动子(prOsU3)控制。这些载体还包含PMI选择性标志物基因盒，用于选择稳定转化体。这些载体可用于转化由土壤杆菌介导的转化介导的转化或直接用于粒子轰击。
5.3 ZmPYL-D，ZmPYL-F和ZmPYL-J基因中基因组编辑的新型等位基因(靶向的诱变和等位基因替代突变)介导的CRISPR-Cas的生成
包括靶向的诱变和等位基因替代突变体的新型等位基因可通过CRISPR-Cas系统在修复供体DNA存在下通过土壤杆菌介导的转化或粒子轰击(如上文在实施例4中关于ZmPYL-E所述)生成。此处提供ZmPYL-D、ZmPYL-F和ZmPYL-J基因中靶向的突变的特定实施例，采用转化载体(表1和图9A-B-11)和带有所需突变(表2和SEQ ID NO:398-410)的修复供体寡脱氧核苷酸的生物弹射共递送。采用具有不同长度、链(编码和非编码模板)或修饰(在末端具有和不具有硫代磷酸酯连接修饰)(SEQ ID NO:397-409)的寡脱氧核苷酸(ODN)来介导不同ZmPYL基因中的诱变。
表2修复供体DNA寡核苷酸序列
更具体地，表达Cas9和gRNA的上述的转化载体(表2)与其对应修复供体DNA寡核苷酸(SEQ ID NO:398-410)混合，然后沉淀至金粒子上。然后，用经被覆的金粒子轰击优良自交转化变体NP2222的未成熟玉米胚(DeFramond AJ,等(2013)，玉米事件5307.美国专利号8,466,346)。其它玉米基因型例如A188和HiII可用作轰击靶标组织来源。用于玉米未成熟胚轰击方法、愈伤组织诱导组织再生方法和生根方法已于先前描述，除了本文不需甘露糖选择(Wright等，2001，采用磷甘露糖异构酶基因pmi作为选择性标志物的玉米(Zea maysL.)和小麦(Triticum aestivum L.)的有效生物弹射转化(Efficient biolistictransformation of maize(Zea mays L.)and wheat(Triticum aestivum L.)using thephosphomannose isomerase gene,pmi,as the selectable marker).Plant CellReports 20:429–436.)。例如，对于由CRISP-Cas9介导的ZmPYL-F基因的诱变，未成熟胚在授粉后约9-12天从收获的未成熟穗分离，并在渗压剂培养基上预培养3-5天。然后，预培养胚用伴随寡核苷酸[ODN-ZmPYL-Fa、ODN-ZmPYL-Fb、ODN-ZmPYL-Fc、ODN-ZmPYL-Fd-NT或ODN-ZmPYL-Fd-NT-PM(Seq.ID.NO:402-406)]之一的DNA载体22981，采用BioRad PDS-1000生物弹射粒子递送系统轰击。然后，被轰击的胚在愈伤组织诱导培养基中孵育，然后移至甘露糖选择培养基上。将选择的愈伤组织移至再生培养基上以诱导成芽。然后将芽移至生根培养基。然后从再生的植物收获样品用于基因分型，以鉴定包含导致ZmPYL-F基因中E164L氨基酸变化的所需基因组序列突变的植物。表3列出用于靶向的诱变和不同ZmPYL基因的等位基因替代的不同实验。在一些实验中，gRNA和Cas9表达载体与ZsGreen荧光蛋白质载体12672共转化，用于评估基因递送效率。在一些其它实验中，两个或更多gRNA表达载体与两个或更多个修复供体寡脱氧核苷酸共递送，以同时突变两个或ZmPYL基因(表3)。表3显示高百分比PMI阳性事件的ZmPYL-F(转化体)在gRNA-Cas9靶向的意图的序列(5’-gctcg tgatc gagtccttcg/tggtg gacgt-3’，/指示预计的Cas9切割位置)处包含突变。
表3不同ZmPYL基因的靶向诱变和等位基因替代实验
选择的突变体的ZmPYL-F靶区域测序确认qPCR结果。图13显示由载体22981表达的gRNA-Cas9介导的ZmPYL-F中的靶向的突变的序列比对。
注：*同时具有两个单等位基因和/或双等位基因突变的事件；**这些载体(22980、22978或22982)携带表达对照gRNA(包括NGG序列)的盒用于测试gRNA对于ZmPYL基因的特异性。#TBD，待测定；实验正在进行，目前尚未有可得数据。
5.4编辑的ZmPYL突变体的分子表征
叶样品从再生的植物根脉管中收获，供于分子分析或基因分型，以鉴定在靶序列处包含突变且还包含导致ZmPYL基因中所需氨基酸变化的所需序列突变的植物。靶向的突变体可采用如下方法之一鉴定：(1)靶区域的PCR扩增，如果突变的序列包含限制性位点，则随后进行限制性酶消化和凝胶电泳，(Lloyd A等2005.Proc.Natl.Acad.Sci.USA 102:2232–37；Zhang F等.2010.Proc.Natl.Acad.Sci.USA 107:12028–33)。该方法简单，但需要存在合适的限制性位点，因此无法用于大多数靶标。(2)靶区域的PCR扩增，然后进行Sanger测序或深度测序(Gross，E.等1999.Hum.Genet.105,72–78.Shukla VK等.2009.Nature459:437-41.Townsend JA等.2009.Nature 459:442–45.)；测序方法是确定且灵敏的，但耗时较久，且通量可能会受容量限制。(3)靶区域的PCR扩增，然后进行变性、退火和毛细管电泳(Li-Sucholeik XC等1999.Electrophoresis 20,1224–1232；Larsen LA等.1999.Hum.Mutat.13,318–327)或变性高效液相色谱，以通过异源双链核酸分子分析检测碱基对变化(McCallum CM等.2000.Nature Biotechnology 18,455–457)；这些方法受限于通量，且鉴定的突变需要通过测序进一步验证。(4)靶区域的PCR扩增，然后进行变性、异源双链核酸分子形成/链退火、用错配特异性核酸酶(例如CEL1和T7内切核酸酶)消化和凝胶电泳(Oleykowski，C.A.等1998.Nucleic Acids Res.26,4597–4602.Colbert等.2001.Plant Physiol.126:480-484；Lombardo A等.2007.Nat.Biotechnol.25:1298–306)，例如，采用市售可得的SurveyerTM核酸酶试验试剂盒(转基因组公司(Transgenomic)，美国马里兰州的盖塞斯堡；Qiu,P.等.2004.BioTechniques 36,702-707)。然而，基于凝胶的试验不如高通量DNA测序灵敏，且仅可检测频率为1％或更多的突变。上述鉴定靶位点中可能的突变的所有四种方法均基于新信号的存在(不同于野生型参比序列的凝胶中的新条带或色谱中的新峰)以定量形式进行。
我们已开发可替代的高通量试验方法，用于基于qPCR来鉴定在靶向的序列中具有任何定点突变的植物(先正达(Syngenta)临时专利申请#9207-137PR，案号80484)。该方法检测已用定点核酸酶处理的细胞或组织中的野生型靶位点序列的减少，以定量形式进行，与参比样品做比较。通常，采用基于Taqman的试验用于定量靶序列拷贝数。对于检测具有所需等位基因替代的可能的事件，设计并进行其它高通量终点试验。在该终点试验中，采用来自两种MGB探针的信号，以确定野生型或预期的突变等位基因的存在，如图12所示。具有推定等位基因替代的事件基于Taqman拷贝数试验(野生型靶序列拷贝数)和终点试验(突变体拷贝数)结果来选择。具有推定等位基因替代的推定事件通过扩增的靶标基因座序列的DNA序列分析来进一步确认。表4显示由与寡核苷酸ODN-ZmPYL-Fb(Seq.ID.No.402)共递送的载体22981(图10A-10B)的生物弹射转化实验产生的选定数量的再生的玉米植物的qPCR和终点试验结果。如表4所示，进行转化载体特异性试验以确定是否存在任何转基因插入(cCas9-01和cPMI-09qPCR试验)。还进行qPCR试验(ZmPYL-F切割位点)以确定ZmPYL-F玉米基因组靶位点序列(5’-gctcg tgatc gagtc cttcg/tgg-3’，SEQ ID NO:394)的拷贝数。最终，还采用终点试验(ZmPYL-F E164L)来确定植物是否具有导致E164L氨基酸残基变化的意图的序列突变(从GAG至CTG)。例如，植物MZET151104A015A具有单一拷贝的转基因插入(对于Cas9和PMI基因)，位于靶序列的双等位基因突变，因为ZmPYL-F切割位点拷贝判定是0(在野生型中，拷贝判定应为2)且无E164L突变。另一植物MZET151104A125A具有多于2个拷贝的转化载体(22981)插入且仅一拷贝的ZmPYL-F切割位点是突变的。但植物MZET151104A125A对用于检测ZmPYL-F E164L突变的终点试验呈阳性。因此，该事件是具有ZmPYL-F E164L突变的候选事件。然后，通过对PCR扩增的ZmPYL-F基因组序列进行测序分析，进一步确认表4中的候选事件MZET151104A125A、MZET151104A174A和MZET151104A180A。
表4再生的玉米事件的qPCR和终点试验结果
5.5 ZmPYL基因编辑的突变体的评价
ZmPYL基因编辑的突变体的测试如对于转基因T6PP玉米植物的描述(Nuccio等，2015，Nature Biotechnology，doi:10.1038/nbt.3277)采用控制的胁迫环境(MSE)试验进行。在多位置农艺等同(Ag Eq)试验中采用伴随对照植物生长并采用棋盘平面布局的突变系，进一步测试显示改善的缺水植物响应的突变系。
应理解，本文所述的实施例和实施方式仅用于说明目的，本领域技术人员应了解据此作出的各种修饰或改变，且它们包括在本申请的主旨和权益以及所附权利要求书的范围内。本文引用的所有发表物、专利和专利申请通过引用全文纳入本文以用于所有目的。
序列表
<110> S·卡特勒(Cutler, Sean R.)
加利福尼亚大学董事会(The Regents of the University of California)
<120> 超敏ABA受体
<130> 81906-956354
<150> US 62/042,095
<151> 2014-08-26
<160> 411
<170> FastSEQ for Windows Version 4.0
<210> 1
<211> 191
<212> PRT
<213> 拟南芥(Arabidopsis thaliana)
<220>
<223> 拟南芥(thale cress)PYR/PYL受体, Pyrabactin抗性1,
脱落酸受体 PYR1 (PYR1), ABI1结合蛋白6
(ABIP6), ABA受体11的调节组分 (RCAR11),
At4g17870, T6K21.50
<400> 1
Met Pro Ser Glu Leu Thr Pro Glu Glu Arg Ser Glu Leu Lys Asn Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Leu Val Trp Ser Ile
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser
50 55 60
Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Val Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val Leu Glu Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Ala
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Ala Glu
165 170 175
Ala Met Ala Arg Asn Ser Gly Asp Gly Ser Gly Ser Gln Val Thr
180 185 190
<210> 2
<211> 221
<212> PRT
<213> 拟南芥(Arabidopsis thaliana)
<220>
<223> 拟南芥(thale cress)PYR/PYL受体, 脱落酸受体 PYL1,
PYR1样蛋白1 (PYL1), ABI1结合蛋白6 (ABIP6),
ABA受体9的调节组分 (RCAR12),
At5g46790, MZA15.21
<400> 2
Met Ala Asn Ser Glu Ser Ser Ser Ser Pro Val Asn Glu Glu Glu Asn
1 5 10 15
Ser Gln Arg Ile Ser Thr Leu His His Gln Thr Met Pro Ser Asp Leu
20 25 30
Thr Gln Asp Glu Phe Thr Gln Leu Ser Gln Ser Ile Ala Glu Phe His
35 40 45
Thr Tyr Gln Leu Gly Asn Gly Arg Cys Ser Ser Leu Leu Ala Gln Arg
50 55 60
Ile His Ala Pro Pro Glu Thr Val Trp Ser Val Val Arg Arg Phe Asp
65 70 75 80
Arg Pro Gln Ile Tyr Lys His Phe Ile Lys Ser Cys Asn Val Ser Glu
85 90 95
Asp Phe Glu Met Arg Val Gly Cys Thr Arg Asp Val Asn Val Ile Ser
100 105 110
Gly Leu Pro Ala Asn Thr Ser Arg Glu Arg Leu Asp Leu Leu Asp Asp
115 120 125
Asp Arg Arg Val Thr Gly Phe Ser Ile Thr Gly Gly Glu His Arg Leu
130 135 140
Arg Asn Tyr Lys Ser Val Thr Thr Val His Arg Phe Glu Lys Glu Glu
145 150 155 160
Glu Glu Glu Arg Ile Trp Thr Val Val Leu Glu Ser Tyr Val Val Asp
165 170 175
Val Pro Glu Gly Asn Ser Glu Glu Asp Thr Arg Leu Phe Ala Asp Thr
180 185 190
Val Ile Arg Leu Asn Leu Gln Lys Leu Ala Ser Ile Thr Glu Ala Met
195 200 205
Asn Arg Asn Asn Asn Asn Asn Asn Ser Ser Gln Val Arg
210 215 220
<210> 3
<211> 190
<212> PRT
<213> 拟南芥(Arabidopsis thaliana)
<220>
<223> 拟南芥(thale cress)PYR/PYL受体, 脱落酸受体 PYL2,
PYR1样蛋白2 (PYL2), ABI1结合蛋白6 (ABIP6),
ABA受体14的调节组分 (RCAR14), Bet v I
过敏原家族蛋白, At2g26040, T19L18.15
<400> 3
Met Ser Ser Ser Pro Ala Val Lys Gly Leu Thr Asp Glu Glu Gln Lys
1 5 10 15
Thr Leu Glu Pro Val Ile Lys Thr Tyr His Gln Phe Glu Pro Asp Pro
20 25 30
Thr Thr Cys Thr Ser Leu Ile Thr Gln Arg Ile His Ala Pro Ala Ser
35 40 45
Val Val Trp Pro Leu Ile Arg Arg Phe Asp Asn Pro Glu Arg Tyr Lys
50 55 60
His Phe Val Lys Arg Cys Arg Leu Ile Ser Gly Asp Gly Asp Val Gly
65 70 75 80
Ser Val Arg Glu Val Thr Val Ile Ser Gly Leu Pro Ala Ser Thr Ser
85 90 95
Thr Glu Arg Leu Glu Phe Val Asp Asp Asp His Arg Val Leu Ser Phe
100 105 110
Arg Val Val Gly Gly Glu His Arg Leu Lys Asn Tyr Lys Ser Val Thr
115 120 125
Ser Val Asn Glu Phe Leu Asn Gln Asp Ser Gly Lys Val Tyr Thr Val
130 135 140
Val Leu Glu Ser Tyr Thr Val Asp Ile Pro Glu Gly Asn Thr Glu Glu
145 150 155 160
Asp Thr Lys Met Phe Val Asp Thr Val Val Lys Leu Asn Leu Gln Lys
165 170 175
Leu Gly Val Ala Ala Thr Ser Ala Pro Met His Asp Asp Glu
180 185 190
<210> 4
<211> 209
<212> PRT
<213> 拟南芥(Arabidopsis thaliana)
<220>
<223> 拟南芥(thale cress)PYR/PYL受体, 脱落酸受体 PYL3,
PYR1样蛋白3 (PYL3), ABA受体13的调节组分 (RCAR13), At1g73000, F3N23.20
<400> 4
Met Asn Leu Ala Pro Ile His Asp Pro Ser Ser Ser Ser Thr Thr Thr
1 5 10 15
Thr Ser Ser Ser Thr Pro Tyr Gly Leu Thr Lys Asp Glu Phe Ser Thr
20 25 30
Leu Asp Ser Ile Ile Arg Thr His His Thr Phe Pro Arg Ser Pro Asn
35 40 45
Thr Cys Thr Ser Leu Ile Ala His Arg Val Asp Ala Pro Ala His Ala
50 55 60
Ile Trp Arg Phe Val Arg Asp Phe Ala Asn Pro Asn Lys Tyr Lys His
65 70 75 80
Phe Ile Lys Ser Cys Thr Ile Arg Val Asn Gly Asn Gly Ile Lys Glu
85 90 95
Ile Lys Val Gly Thr Ile Arg Glu Val Ser Val Val Ser Gly Leu Pro
100 105 110
Ala Ser Thr Ser Val Glu Ile Leu Glu Val Leu Asp Glu Glu Lys Arg
115 120 125
Ile Leu Ser Phe Arg Val Leu Gly Gly Glu His Arg Leu Asn Asn Tyr
130 135 140
Arg Ser Val Thr Ser Val Asn Glu Phe Val Val Leu Glu Lys Asp Lys
145 150 155 160
Lys Lys Arg Val Tyr Ser Val Val Leu Glu Ser Tyr Ile Val Asp Ile
165 170 175
Pro Gln Gly Asn Thr Glu Glu Asp Thr Arg Met Phe Val Asp Thr Val
180 185 190
Val Lys Ser Asn Leu Gln Asn Leu Ala Val Ile Ser Thr Ala Ser Pro
195 200 205
Thr
<210> 5
<211> 207
<212> PRT
<213> 拟南芥(Arabidopsis thaliana)
<220>
<223> 拟南芥(thale cress)PYR/PYL受体, 脱落酸受体 PYL4,
PYR1样蛋白4 (PYL4), ABI1结合蛋白2 (ABIP2),
ABA受体10的调节组分 (RCAR10),
At2g38310, T19C21.20
<400> 5
Met Leu Ala Val His Arg Pro Ser Ser Ala Val Ser Asp Gly Asp Ser
1 5 10 15
Val Gln Ile Pro Met Met Ile Ala Ser Phe Gln Lys Arg Phe Pro Ser
20 25 30
Leu Ser Arg Asp Ser Thr Ala Ala Arg Phe His Thr His Glu Val Gly
35 40 45
Pro Asn Gln Cys Cys Ser Ala Val Ile Gln Glu Ile Ser Ala Pro Ile
50 55 60
Ser Thr Val Trp Ser Val Val Arg Arg Phe Asp Asn Pro Gln Ala Tyr
65 70 75 80
Lys His Phe Leu Lys Ser Cys Ser Val Ile Gly Gly Asp Gly Asp Asn
85 90 95
Val Gly Ser Leu Arg Gln Val His Val Val Ser Gly Leu Pro Ala Ala
100 105 110
Ser Ser Thr Glu Arg Leu Asp Ile Leu Asp Asp Glu Arg His Val Ile
115 120 125
Ser Phe Ser Val Val Gly Gly Asp His Arg Leu Ser Asn Tyr Arg Ser
130 135 140
Val Thr Thr Leu His Pro Ser Pro Ile Ser Gly Thr Val Val Val Glu
145 150 155 160
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Lys Glu Glu Thr Cys
165 170 175
Asp Phe Val Asp Val Ile Val Arg Cys Asn Leu Gln Ser Leu Ala Lys
180 185 190
Ile Ala Glu Asn Thr Ala Ala Glu Ser Lys Lys Lys Met Ser Leu
195 200 205
<210> 6
<211> 203
<212> PRT
<213> 拟南芥(Arabidopsis thaliana)
<220>
<223> 拟南芥(thale cress)PYR/PYL受体, 脱落酸受体 PYL5,
PYR1样蛋白5 (PYL5), ABI1结合蛋白3 (ABIP3),
ABA受体8的调节组分 (RCAR8), Bet v I
过敏原家族蛋白, At5g05440, K18I23.25
<400> 6
Met Arg Ser Pro Val Gln Leu Gln His Gly Ser Asp Ala Thr Asn Gly
1 5 10 15
Phe His Thr Leu Gln Pro His Asp Gln Thr Asp Gly Pro Ile Lys Arg
20 25 30
Val Cys Leu Thr Arg Gly Met His Val Pro Glu His Val Ala Met His
35 40 45
His Thr His Asp Val Gly Pro Asp Gln Cys Cys Ser Ser Val Val Gln
50 55 60
Met Ile His Ala Pro Pro Glu Ser Val Trp Ala Leu Val Arg Arg Phe
65 70 75 80
Asp Asn Pro Lys Val Tyr Lys Asn Phe Ile Arg Gln Cys Arg Ile Val
85 90 95
Gln Gly Asp Gly Leu His Val Gly Asp Leu Arg Glu Val Met Val Val
100 105 110
Ser Gly Leu Pro Ala Val Ser Ser Thr Glu Arg Leu Glu Ile Leu Asp
115 120 125
Glu Glu Arg His Val Ile Ser Phe Ser Val Val Gly Gly Asp His Arg
130 135 140
Leu Lys Asn Tyr Arg Ser Val Thr Thr Leu His Ala Ser Asp Asp Glu
145 150 155 160
Gly Thr Val Val Val Glu Ser Tyr Ile Val Asp Val Pro Pro Gly Asn
165 170 175
Thr Glu Glu Glu Thr Leu Ser Phe Val Asp Thr Ile Val Arg Cys Asn
180 185 190
Leu Gln Ser Leu Ala Arg Ser Thr Asn Arg Gln
195 200
<210> 7
<211> 215
<212> PRT
<213> 拟南芥(Arabidopsis thaliana)
<220>
<223> 拟南芥(thale cress)PYR/PYL受体, 脱落酸受体 PYL6,
PYR1样蛋白6 (PYL6), ABI1结合蛋白5 (ABIP5),
ABA受体9的调节组分 (RCAR9), Bet v I
过敏原家族蛋白, At2g40330, T7M7.15
<400> 7
Met Pro Thr Ser Ile Gln Phe Gln Arg Ser Ser Thr Ala Ala Glu Ala
1 5 10 15
Ala Asn Ala Thr Val Arg Asn Tyr Pro His His His Gln Lys Gln Val
20 25 30
Gln Lys Val Ser Leu Thr Arg Gly Met Ala Asp Val Pro Glu His Val
35 40 45
Glu Leu Ser His Thr His Val Val Gly Pro Ser Gln Cys Phe Ser Val
50 55 60
Val Val Gln Asp Val Glu Ala Pro Val Ser Thr Val Trp Ser Ile Leu
65 70 75 80
Ser Arg Phe Glu His Pro Gln Ala Tyr Lys His Phe Val Lys Ser Cys
85 90 95
His Val Val Ile Gly Asp Gly Arg Glu Val Gly Ser Val Arg Glu Val
100 105 110
Arg Val Val Ser Gly Leu Pro Ala Ala Phe Ser Leu Glu Arg Leu Glu
115 120 125
Ile Met Asp Asp Asp Arg His Val Ile Ser Phe Ser Val Val Gly Gly
130 135 140
Asp His Arg Leu Met Asn Tyr Lys Ser Val Thr Thr Val His Glu Ser
145 150 155 160
Glu Glu Asp Ser Asp Gly Lys Lys Arg Thr Arg Val Val Glu Ser Tyr
165 170 175
Val Val Asp Val Pro Ala Gly Asn Asp Lys Glu Glu Thr Cys Ser Phe
180 185 190
Ala Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Ala Lys Leu Ala
195 200 205
Glu Asn Thr Ser Lys Phe Ser
210 215
<210> 8
<211> 211
<212> PRT
<213> 拟南芥(Arabidopsis thaliana)
<220>
<223> 拟南芥(thale cress)PYR/PYL受体, 脱落酸受体 PYL7,
PYR1样蛋白7 (PYL7), ABI1结合蛋白7 (ABIP7),
ABA受体2的调节组分 (RCAR2), At4g01026
<400> 8
Met Glu Met Ile Gly Gly Asp Asp Thr Asp Thr Glu Met Tyr Gly Ala
1 5 10 15
Leu Val Thr Ala Gln Ser Leu Arg Leu Arg His Leu His His Cys Arg
20 25 30
Glu Asn Gln Cys Thr Ser Val Leu Val Lys Tyr Ile Gln Ala Pro Val
35 40 45
His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Lys Tyr
50 55 60
Lys Pro Phe Ile Ser Arg Cys Thr Val Asn Gly Asp Pro Glu Ile Gly
65 70 75 80
Cys Leu Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala Thr Thr Ser
85 90 95
Thr Glu Arg Leu Glu Gln Leu Asp Asp Glu Glu His Ile Leu Gly Ile
100 105 110
Asn Ile Ile Gly Gly Asp His Arg Leu Lys Asn Tyr Ser Ser Ile Leu
115 120 125
Thr Val His Pro Glu Met Ile Asp Gly Arg Ser Gly Thr Met Val Met
130 135 140
Glu Ser Phe Val Val Asp Val Pro Gln Gly Asn Thr Lys Asp Asp Thr
145 150 155 160
Cys Tyr Phe Val Glu Ser Leu Ile Lys Cys Asn Leu Lys Ser Leu Ala
165 170 175
Cys Val Ser Glu Arg Leu Ala Ala Gln Asp Ile Thr Asn Ser Ile Ala
180 185 190
Thr Phe Cys Asn Ala Ser Asn Gly Tyr Arg Glu Lys Asn His Thr Glu
195 200 205
Thr Asn Leu
210
<210> 9
<211> 188
<212> PRT
<213> 拟南芥(Arabidopsis thaliana)
<220>
<223> 拟南芥(thale cress)PYR/PYL受体, 脱落酸受体 PYL8,
PYR1样蛋白8 (PYL8), ABI1结合蛋白1 (ABIP1),
ABA受体3的调节组分 (RCAR3), At5g53160,
MFH8.10
<400> 9
Met Glu Ala Asn Gly Ile Glu Asn Leu Thr Asn Pro Asn Gln Glu Arg
1 5 10 15
Glu Phe Ile Arg Arg His His Lys His Glu Leu Val Asp Asn Gln Cys
20 25 30
Ser Ser Thr Leu Val Lys His Ile Asn Ala Pro Val His Ile Val Trp
35 40 45
Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Lys Tyr Lys Pro Phe Ile
50 55 60
Ser Arg Cys Val Val Lys Gly Asn Met Glu Ile Gly Thr Val Arg Glu
65 70 75 80
Val Asp Val Lys Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu
85 90 95
Glu Leu Leu Asp Asp Asn Glu His Ile Leu Ser Ile Arg Ile Val Gly
100 105 110
Gly Asp His Arg Leu Lys Asn Tyr Ser Ser Ile Ile Ser Leu His Pro
115 120 125
Glu Thr Ile Glu Gly Arg Ile Gly Thr Leu Val Ile Glu Ser Phe Val
130 135 140
Val Asp Val Pro Glu Gly Asn Thr Lys Asp Glu Thr Cys Tyr Phe Val
145 150 155 160
Glu Ala Leu Ile Lys Cys Asn Leu Lys Ser Leu Ala Asp Ile Ser Glu
165 170 175
Arg Leu Ala Val Gln Asp Thr Thr Glu Ser Arg Val
180 185
<210> 10
<211> 187
<212> PRT
<213> 拟南芥(Arabidopsis thaliana)
<220>
<223> 拟南芥(thale cress)PYR/PYL受体, 脱落酸受体 PYL9,
PYR1样蛋白9 (PYL9), ABI1结合蛋白4 (ABIP4),
ABA受体1的调节组分 (RCAR1), At1g01360,
F6F3.16
<400> 10
Met Met Asp Gly Val Glu Gly Gly Thr Ala Met Tyr Gly Gly Leu Glu
1 5 10 15
Thr Val Gln Tyr Val Arg Thr His His Gln His Leu Cys Arg Glu Asn
20 25 30
Gln Cys Thr Ser Ala Leu Val Lys His Ile Lys Ala Pro Leu His Leu
35 40 45
Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Lys Tyr Lys Pro
50 55 60
Phe Val Ser Arg Cys Thr Val Ile Gly Asp Pro Glu Ile Gly Ser Leu
65 70 75 80
Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala Thr Thr Ser Thr Glu
85 90 95
Arg Leu Glu Leu Leu Asp Asp Glu Glu His Ile Leu Gly Ile Lys Ile
100 105 110
Ile Gly Gly Asp His Arg Leu Lys Asn Tyr Ser Ser Ile Leu Thr Val
115 120 125
His Pro Glu Ile Ile Glu Gly Arg Ala Gly Thr Met Val Ile Glu Ser
130 135 140
Phe Val Val Asp Val Pro Gln Gly Asn Thr Lys Asp Glu Thr Cys Tyr
145 150 155 160
Phe Val Glu Ala Leu Ile Arg Cys Asn Leu Lys Ser Leu Ala Asp Val
165 170 175
Ser Glu Arg Leu Ala Ser Gln Asp Ile Thr Gln
180 185
<210> 11
<211> 183
<212> PRT
<213> 拟南芥(Arabidopsis thaliana)
<220>
<223> 拟南芥(thale cress)PYR/PYL受体, 脱落酸受体 PYL10,
PYR1样蛋白10 (PYL10), ABI1结合蛋白8 (ABIP8),
ABA受体4的调节组分 (RCAR4), At4g27920,
T13J8.30
<400> 11
Met Asn Gly Asp Glu Thr Lys Lys Val Glu Ser Glu Tyr Ile Lys Lys
1 5 10 15
His His Arg His Glu Leu Val Glu Ser Gln Cys Ser Ser Thr Leu Val
20 25 30
Lys His Ile Lys Ala Pro Leu His Leu Val Trp Ser Ile Val Arg Arg
35 40 45
Phe Asp Glu Pro Gln Lys Tyr Lys Pro Phe Ile Ser Arg Cys Val Val
50 55 60
Gln Gly Lys Lys Leu Glu Val Gly Ser Val Arg Glu Val Asp Leu Lys
65 70 75 80
Ser Gly Leu Pro Ala Thr Lys Ser Thr Glu Val Leu Glu Ile Leu Asp
85 90 95
Asp Asn Glu His Ile Leu Gly Ile Arg Ile Val Gly Gly Asp His Arg
100 105 110
Leu Lys Asn Tyr Ser Ser Thr Ile Ser Leu His Ser Glu Thr Ile Asp
115 120 125
Gly Lys Thr Gly Thr Leu Ala Ile Glu Ser Phe Val Val Asp Val Pro
130 135 140
Glu Gly Asn Thr Lys Glu Glu Thr Cys Phe Phe Val Glu Ala Leu Ile
145 150 155 160
Gln Cys Asn Leu Asn Ser Leu Ala Asp Val Thr Glu Arg Leu Gln Ala
165 170 175
Glu Ser Met Glu Lys Lys Ile
180
<210> 12
<211> 161
<212> PRT
<213> 拟南芥(Arabidopsis thaliana)
<220>
<223> 拟南芥(thale cress)PYR/PYL受体, 脱落酸受体 PYL11,
PYR1样蛋白11 (PYL11), ABA受体5的调节组分(RCAR5), Bet v I 过敏原家族蛋白,
At5g45860, K15I22.6
<400> 12
Met Glu Thr Ser Gln Lys Tyr His Thr Cys Gly Ser Thr Leu Val Gln
1 5 10 15
Thr Ile Asp Ala Pro Leu Ser Leu Val Trp Ser Ile Leu Arg Arg Phe
20 25 30
Asp Asn Pro Gln Ala Tyr Lys Gln Phe Val Lys Thr Cys Asn Leu Ser
35 40 45
Ser Gly Asp Gly Gly Glu Gly Ser Val Arg Glu Val Thr Val Val Ser
50 55 60
Gly Leu Pro Ala Glu Phe Ser Arg Glu Arg Leu Asp Glu Leu Asp Asp
65 70 75 80
Glu Ser His Val Met Met Ile Ser Ile Ile Gly Gly Asp His Arg Leu
85 90 95
Val Asn Tyr Arg Ser Lys Thr Met Ala Phe Val Ala Ala Asp Thr Glu
100 105 110
Glu Lys Thr Val Val Val Glu Ser Tyr Val Val Asp Val Pro Glu Gly
115 120 125
Asn Ser Glu Glu Glu Thr Thr Ser Phe Ala Asp Thr Ile Val Gly Phe
130 135 140
Asn Leu Lys Ser Leu Ala Lys Leu Ser Glu Arg Val Ala His Leu Lys
145 150 155 160
Leu
<210> 13
<211> 159
<212> PRT
<213> 拟南芥(Arabidopsis thaliana)
<220>
<223> 拟南芥(thale cress)PYR/PYL受体, 脱落酸受体 PYL12,
PYR1样蛋白12 (PYL12), ABA受体6的调节组分(RCAR6), Bet v I 过敏原家族蛋白,
At5g45870, K15I22.7
<400> 13
Met Lys Thr Ser Gln Glu Gln His Val Cys Gly Ser Thr Val Val Gln
1 5 10 15
Thr Ile Asn Ala Pro Leu Pro Leu Val Trp Ser Ile Leu Arg Arg Phe
20 25 30
Asp Asn Pro Lys Thr Phe Lys His Phe Val Lys Thr Cys Lys Leu Arg
35 40 45
Ser Gly Asp Gly Gly Glu Gly Ser Val Arg Glu Val Thr Val Val Ser
50 55 60
Asp Leu Pro Ala Ser Phe Ser Leu Glu Arg Leu Asp Glu Leu Asp Asp
65 70 75 80
Glu Ser His Val Met Val Ile Ser Ile Ile Gly Gly Asp His Arg Leu
85 90 95
Val Asn Tyr Gln Ser Lys Thr Thr Val Phe Val Ala Ala Glu Glu Glu
100 105 110
Lys Thr Val Val Val Glu Ser Tyr Val Val Asp Val Pro Glu Gly Asn
115 120 125
Thr Glu Glu Glu Thr Thr Leu Phe Ala Asp Thr Ile Val Gly Cys Asn
130 135 140
Leu Arg Ser Leu Ala Lys Leu Ser Glu Lys Met Met Glu Leu Thr
145 150 155
<210> 14
<211> 164
<212> PRT
<213> 拟南芥(Arabidopsis thaliana)
<220>
<223> 拟南芥(thale cress)PYR/PYL受体, 脱落酸受体 PYL13,
PYR1样蛋白13 (PYL13), ABA受体7的调节组分(RCAR7), At4g18620, F28A21.30
<400> 14
Met Glu Ser Ser Lys Gln Lys Arg Cys Arg Ser Ser Val Val Glu Thr
1 5 10 15
Ile Glu Ala Pro Leu Pro Leu Val Trp Ser Ile Leu Arg Ser Phe Asp
20 25 30
Lys Pro Gln Ala Tyr Gln Arg Phe Val Lys Ser Cys Thr Met Arg Ser
35 40 45
Gly Gly Gly Gly Gly Lys Gly Gly Glu Gly Lys Gly Ser Val Arg Asp
50 55 60
Val Thr Leu Val Ser Gly Phe Pro Ala Asp Phe Ser Thr Glu Arg Leu
65 70 75 80
Glu Glu Leu Asp Asp Glu Ser His Val Met Val Val Ser Ile Ile Gly
85 90 95
Gly Asn His Arg Leu Val Asn Tyr Lys Ser Lys Thr Lys Val Val Ala
100 105 110
Ser Pro Glu Asp Met Ala Lys Lys Thr Val Val Val Glu Ser Tyr Val
115 120 125
Val Asp Val Pro Glu Gly Thr Ser Glu Glu Asp Thr Ile Phe Phe Val
130 135 140
Asp Asn Ile Ile Arg Tyr Asn Leu Thr Ser Leu Ala Lys Leu Thr Lys
145 150 155 160
Lys Met Met Lys
<210> 15
<211> 191
<212> PRT
<213> 甘蓝(Brassica oleracea)
<220>
<223> 野生甘蓝链霉菌环化酶/脱氢酶家族蛋白,
基因座标签40.t00062, GenBank登录号ABD65175.1
<400> 15
Met Pro Ser Gln Leu Thr Pro Glu Glu Arg Ser Glu Leu Ala Gln Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr His Leu Gly Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Ile Val Trp Ser Val
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser
50 55 60
Cys Ser Val Glu Asp Gly Phe Glu Met Arg Val Gly Cys Thr Arg Ala
65 70 75 80
Val Asn Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Arg Arg Ile Trp Thr Val Val Leu Glu Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Ala
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Thr Glu
165 170 175
Ala Met Ala Arg Asn Ala Gly Asp Gly Ser Gly Ala Gln Val Thr
180 185 190
<210> 16
<211> 281
<212> PRT
<213> 甘蓝(Brassica oleracea)
<220>
<223> 野生甘蓝链霉菌环化酶/脱氢酶家族蛋白,
基因座标签23.t00047, GenBank登录号ABD65631.1
<400> 16
Met Pro Ser Glu Leu Thr Gln Glu Glu Arg Ser Lys Leu Thr Gln Ser
1 5 10 15
Ile Ser Glu Phe His Thr Tyr His Leu Gly Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Ile Val Trp Ser Val
35 40 45
Val Arg Gln Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser
50 55 60
Cys Ser Val Glu Glu Gly Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Val Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Met Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly
100 105 110
Gly Glu His Arg Leu Lys Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Arg Glu Arg Arg Ile Trp Thr Val Val Leu Glu Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Ala
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Thr Glu
165 170 175
Ala Met Ala Arg Asn Ala Gly Asp Gly Arg Gly Ser Arg Glu Thr Thr
180 185 190
Cys Arg Glu Ser Phe His Leu Ile Thr Ala Phe Glu Lys Gln Arg Gln
195 200 205
Ile Thr Glu Pro Thr Val Tyr Gln Asn Pro Pro Tyr His Thr Gly Met
210 215 220
Thr Pro Glu Pro Arg Thr Ser Thr Val Phe Ile Glu Leu Glu Asp His
225 230 235 240
Arg Thr Leu Pro Gly Asn Leu Thr Pro Thr Thr Glu Glu His Leu Gln
245 250 255
Arg Met Tyr Gln Arg Phe Trp Gly Ile Arg Gln Leu Gln Arg Pro Arg
260 265 270
Gln Ser Phe Gly Glu Arg Gln Ser Ile
275 280
<210> 17
<211> 453
<212> PRT
<213> 葡萄(Vitis vinifera)
<220>
<223> 酿酒葡萄PN40024未命名蛋白质产物, 基因座
标签GSVIVT00015766001, GenBank登录号CAO63410.1
<400> 17
Met Gln Met Lys Tyr Leu Glu Gly Lys Gln Asn Leu Met Glu Glu Lys
1 5 10 15
Gly Glu Lys Gln Cys Ile Pro Met Asp Leu Ala Val Arg Glu Ala Gln
20 25 30
Phe Lys Gly Ser Leu Leu Asp Arg Ile Thr Trp Leu Glu Gln Arg Leu
35 40 45
His Lys Leu Ser Leu Gln Leu Glu Thr Arg Ser Lys Gln Gln Pro His
50 55 60
Pro Ser Arg Met Gln Thr Ala Gly Glu Thr Ser Ser Arg His Gly Pro
65 70 75 80
Lys Lys Glu Leu Ser Cys Ser Phe Pro Val Phe Ser Thr Arg Asn His
85 90 95
Asn His Gly His Lys Gln Thr Ser Gln Phe His Val Pro Arg Phe Glu
100 105 110
Tyr Gln Glu Gly Gly Arg Glu Asn Pro Ala Val Val Ile Thr Lys Leu
115 120 125
Thr Pro Phe His His Pro Lys Ile Ile Thr Ile Leu Phe Pro Ile Ser
130 135 140
Asn Tyr Phe Ile Ile Phe Phe Phe Leu Thr Phe Asp Thr Lys Lys Gln
145 150 155 160
Tyr Pro Leu Leu Phe Pro Ile Leu Pro Ser Arg Phe Leu Pro Ile Ser
165 170 175
His Leu Ile Thr Gln Glu Ile Glu Lys Tyr Lys Thr Ser Ser His Phe
180 185 190
Ser Ser Pro Ala Ser Leu Phe Ala Ala Met Asn Lys Ala Glu Thr Ser
195 200 205
Ser Met Ala Glu Ala Glu Ser Glu Asp Ser Glu Thr Thr Thr Pro Thr
210 215 220
Thr His His Leu Thr Ile Pro Pro Gly Leu Thr Gln Pro Glu Phe Gln
225 230 235 240
Glu Leu Ala His Ser Ile Ser Glu Phe His Thr Tyr Gln Val Gly Pro
245 250 255
Gly Gln Cys Ser Ser Leu Leu Ala Gln Arg Val His Ala Pro Leu Pro
260 265 270
Thr Val Trp Ser Val Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys
275 280 285
His Phe Ile Lys Ser Cys His Val Glu Asp Gly Phe Glu Met Arg Val
290 295 300
Gly Cys Leu Arg Asp Val Asn Val Ile Ser Gly Leu Pro Ala Glu Thr
305 310 315 320
Ser Thr Glu Arg Leu Asp Ile Leu Asp Asp Glu Arg His Val Thr Gly
325 330 335
Phe Ser Ile Ile Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val
340 345 350
Thr Thr Asn His Gly Gly Glu Ile Trp Thr Val Val Leu Glu Ser Tyr
355 360 365
Val Val Asp Met Pro Glu Gly Asn Thr Glu Glu Asp Thr Arg Leu Phe
370 375 380
Ala Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Ser Val Thr
385 390 395 400
Glu Val Ser Gln Ser Cys Asn Tyr Pro Cys Gln Phe His Ile Ile Glu
405 410 415
Asn Glu Asp Ile Gln Pro Glu Glu Met Asn Leu Gly Val Leu Thr Thr
420 425 430
Ser Ile Glu Glu Gln Arg Lys Lys Lys Arg Val Val Ala Met Lys Asp
435 440 445
Gly Ser Thr Ser Ser
450
<210> 18
<211> 195
<212> PRT
<213> 葡萄(Vitis vinifera)
<220>
<223> 酿酒葡萄黑皮诺假设蛋白, 克隆
ENTAV 115, 基因座标签VITISV_033963, GenBank登录号
CAN64657.1
<220>
<221> 变体
<222> (193)...(193)
<223> Xaa = 任何氨基酸
<400> 18
Met Ala Glu Ala Glu Ser Glu Asp Ser Glu Thr Thr Thr Pro Thr Thr
1 5 10 15
His His Leu Thr Ile Pro Pro Gly Leu Thr Gln Pro Glu Phe Gln Glu
20 25 30
Leu Ala His Ser Ile Ser Glu Phe His Thr Tyr Gln Val Gly Pro Gly
35 40 45
Gln Cys Ser Ser Leu Leu Ala Gln Arg Val His Ala Pro Leu Pro Thr
50 55 60
Val Trp Ser Val Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His
65 70 75 80
Phe Ile Lys Ser Cys His Val Glu Asp Gly Phe Glu Met Arg Val Gly
85 90 95
Cys Leu Arg Asp Val Asn Val Ile Ser Gly Leu Pro Ala Glu Thr Ser
100 105 110
Thr Glu Arg Leu Asp Ile Leu Asp Asp Glu Arg His Val Thr Gly Phe
115 120 125
Ser Ile Ile Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr
130 135 140
Thr Val His Glu Tyr Gln Asn His Gly Gly Glu Ile Trp Thr Val Val
145 150 155 160
Leu Glu Ser Tyr Val Val Asp Met Pro Glu Gly Asn Thr Glu Glu Asp
165 170 175
Thr Arg Leu Phe Ala Asp Thr Val Val Lys Leu Asn Leu Ser Glu Ala
180 185 190
Xaa Arg Arg
195
<210> 19
<211> 217
<212> PRT
<213> 蒺藜状苜蓿(Medicago truncatula)
<220>
<223> 蒺藜状苜蓿未知蛋白, 克隆 MTYFD_FE_FF_FG1G-N-24,
GenBank登录号ACJ85026.1
<400> 19
Met Glu Lys Ala Glu Ser Ser Thr Ala Ser Thr Ser Asp Gln Asp Ser
1 5 10 15
Asp Glu Asn His Arg Thr Gln His His Leu Thr Leu Pro Ser Gly Leu
20 25 30
Arg Gln His Glu Phe Asp Ser Leu Ile Pro Phe Ile Asn Ser His His
35 40 45
Thr Tyr Leu Ile Gly Pro Asn Gln Cys Ser Thr Leu Leu Ala Gln Arg
50 55 60
Ile His Ala Pro Pro Gln Thr Val Trp Ser Val Val Arg Ser Phe Asp
65 70 75 80
Lys Pro Gln Ile Tyr Lys His Ile Ile Lys Ser Cys Ser Leu Lys Glu
85 90 95
Gly Phe Gln Met Lys Val Gly Cys Thr Arg Asp Val Asn Val Ile Ser
100 105 110
Gly Leu Pro Ala Ala Thr Ser Thr Glu Arg Leu Asp Val Leu Asp Asp
115 120 125
Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly Gly Glu His Arg Leu
130 135 140
Lys Asn Tyr Arg Ser Val Thr Ser Val His Gly Phe Gly Asp Gly Asp
145 150 155 160
Asn Gly Gly Glu Ile Trp Thr Val Val Leu Glu Ser Tyr Val Val Asp
165 170 175
Val Pro Glu Gly Asn Thr Glu Glu Asp Thr Arg Leu Phe Ala Asp Thr
180 185 190
Val Val Lys Leu Asn Leu Gln Lys Leu Ala Ser Val Thr Glu Gly Lys
195 200 205
Asn Arg Asp Gly Asp Gly Lys Ser His
210 215
<210> 20
<211> 212
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻粳组, 日本晴品种, 保守
假设蛋白 Os10g0573400, GenBank登录号
NP_00106570.1
<400> 20
Met Glu Gln Gln Glu Glu Val Pro Pro Pro Pro Ala Gly Leu Gly Leu
1 5 10 15
Thr Ala Glu Glu Tyr Ala Gln Val Arg Ala Thr Val Glu Ala His His
20 25 30
Arg Tyr Ala Val Gly Pro Gly Gln Cys Ser Ser Leu Leu Ala Gln Arg
35 40 45
Ile His Ala Pro Pro Ala Ala Val Trp Ala Val Val Arg Arg Phe Asp
50 55 60
Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Val Leu Arg Pro
65 70 75 80
Asp Pro His His Asp Asp Asn Gly Asn Asp Leu Arg Pro Gly Arg Leu
85 90 95
Arg Glu Val Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu
100 105 110
Arg Leu Asp Leu Leu Asp Asp Ala His Arg Val Phe Gly Phe Thr Ile
115 120 125
Thr Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val
130 135 140
Ser Gln Leu Asp Glu Ile Cys Thr Leu Val Leu Glu Ser Tyr Ile Val
145 150 155 160
Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala Asp
165 170 175
Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ser Glu Ala
180 185 190
Asn Ala Asn Ala Ala Ala Ala Ala Ala Ala Pro Pro Pro Pro Pro Pro
195 200 205
Ala Ala Ala Glu
210
<210> 21
<211> 212
<212> PRT
<213> 玉米(Zea mays)
<220>
<223> 玉米环化酶/脱氢酶家族蛋白, 克隆 306819,
GenBank登录号ACG40002.1
<400> 21
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Val Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Val Pro Ala Ile Cys Thr Val Val Leu Glu Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 22
<211> 212
<212> PRT
<213> 玉米(Zea mays)
<220>
<223> 玉米环化酶/脱氢酶家族蛋白, 克隆 241996,
GenBank登录号ACG34473.1
<220>
<221> 变体
<222> (11)...(11)
<223> Xaa = 任何氨基酸
<400> 22
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Xaa Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Val Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Asp Pro Ala Ile Cys Thr Val Val Leu Glu Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Thr Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 23
<211> 233
<212> PRT
<213> 葡萄(Vitis vinifera)
<220>
<223> 酿酒葡萄PN40024未命名蛋白质产物, 基因座
标签GSVIVT00032173001, GenBank登录号CAO43790.1
<400> 23
Met Asp Pro His His His His Gly Leu Thr Glu Glu Glu Phe Arg Ala
1 5 10 15
Leu Glu Pro Ile Ile Gln Asn Tyr His Thr Phe Glu Pro Ser Pro Asn
20 25 30
Thr Cys Thr Ser Leu Ile Thr Gln Lys Ile Asp Ala Pro Ala Gln Val
35 40 45
Val Trp Pro Phe Val Arg Ser Phe Glu Asn Pro Gln Lys Tyr Lys His
50 55 60
Phe Ile Lys Asp Cys Thr Met Arg Gly Asp Gly Gly Val Gly Ser Ile
65 70 75 80
Arg Glu Val Thr Val Val Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu
85 90 95
Arg Leu Glu Ile Leu Asp Asp Glu Lys His Ile Leu Ser Phe Arg Val
100 105 110
Val Gly Gly Glu His Arg Leu Asn Asn Tyr Arg Ser Val Thr Ser Val
115 120 125
Asn Asp Phe Ser Lys Glu Gly Lys Asp Tyr Thr Ile Val Leu Glu Ser
130 135 140
Tyr Ile Val Asp Ile Pro Glu Gly Asn Thr Gly Glu Asp Thr Lys Met
145 150 155 160
Phe Val Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Val Val
165 170 175
Ala Ile Thr Ser Leu His Glu Asn Glu Glu Ile Ala Asp Asn Glu Gly
180 185 190
Pro Ser Arg Glu Ile Ser Leu Gln Ser Glu Thr Glu Ser Ala Glu Arg
195 200 205
Gly Asp Glu Arg Arg Asp Gly Asp Gly Pro Ser Lys Ala Cys Asn Arg
210 215 220
Asn Glu Trp His Cys Thr Thr Lys Glu
225 230
<210> 24
<211> 207
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻粳组, 日本晴品种, Bet v I
过敏原样蛋白, 克隆 P0495C02.29, GenBank
登录号BAD25659.1
<400> 24
Met Glu Pro His Met Glu Arg Ala Leu Arg Glu Ala Val Ala Ser Glu
1 5 10 15
Ala Glu Arg Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe
20 25 30
Pro Ala Ala Glu Arg Ala Ala Gly Pro Gly Arg Arg Pro Thr Cys Thr
35 40 45
Ser Leu Val Ala Gln Arg Val Asp Ala Pro Leu Ala Ala Val Trp Pro
50 55 60
Ile Val Arg Gly Phe Ala Asn Pro Gln Arg Tyr Lys His Phe Ile Lys
65 70 75 80
Ser Cys Glu Leu Ala Ala Gly Asp Gly Ala Thr Val Gly Ser Val Arg
85 90 95
Glu Val Ala Val Val Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Glu Ile Leu Asp Asp Asp Arg His Val Leu Ser Phe Arg Val Val
115 120 125
Gly Gly Asp His Arg Leu Arg Asn Tyr Arg Ser Val Thr Ser Val Thr
130 135 140
Glu Phe Ser Ser Pro Ser Ser Pro Pro Arg Pro Tyr Cys Val Val Val
145 150 155 160
Glu Ser Tyr Val Val Asp Val Pro Glu Gly Asn Thr Glu Glu Asp Thr
165 170 175
Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala
180 185 190
Ala Val Ala Thr Ser Ser Ser Pro Pro Ala Ala Gly Asn His His
195 200 205
<210> 25
<211> 210
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻籼组, 品种93-11, 假设蛋白
OsI_06433, GenBank登录号EAY85077.1
<400> 25
Met Glu Pro His Met Glu Arg Ala Leu Arg Glu Ala Val Ala Ser Glu
1 5 10 15
Ala Glu Arg Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe
20 25 30
Pro Ala Ala Glu Arg Ala Ala Gly Pro Gly Arg Arg Pro Thr Cys Thr
35 40 45
Ser Leu Val Ala Gln Arg Val Asp Ala Pro Leu Ala Ala Val Trp Pro
50 55 60
Ile Val Arg Gly Phe Ala Asn Pro Gln Arg Tyr Lys His Phe Ile Lys
65 70 75 80
Ser Cys Glu Leu Ala Ala Gly Asp Gly Ala Thr Val Gly Ser Val Arg
85 90 95
Glu Val Ala Val Val Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Glu Ile Leu Asp Asp Asp Arg His Val Leu Ser Phe Arg Val Val
115 120 125
Gly Gly Asp His Arg Leu Arg Asn Tyr Arg Ser Val Thr Ser Val Thr
130 135 140
Glu Phe Ser Ser Pro Ser Ser Pro Pro Ser Pro Pro Arg Pro Tyr Cys
145 150 155 160
Val Val Val Glu Ser Tyr Val Val Asp Val Pro Glu Gly Asn Thr Glu
165 170 175
Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln
180 185 190
Lys Leu Ala Ala Val Ala Thr Ser Ser Ser Pro Pro Ala Ala Gly Asn
195 200 205
His His
210
<210> 26
<211> 200
<212> PRT
<213> 玉米(Zea mays)
<220>
<223> 玉米株B73 未知蛋白, 克隆 ZM_BFb0151H07,
GenBank登录号ACF82013.1
<400> 26
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Leu Ser Gly Gly Gly Ala Lys Ala Ala Ser His Gly Ala Ser Cys Ala
20 25 30
Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala Ala Arg Ala
35 40 45
Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala Pro Val Gly
50 55 60
Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys
65 70 75 80
His Phe Ile Arg Ser Cys Arg Leu Val Gly Gly Gly Asp Val Ala Val
85 90 95
Gly Ser Val Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Thr Ser
100 105 110
Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser
115 120 125
Phe Arg Val Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val
130 135 140
Thr Thr Val His Glu Ala Gly Ala Gly Ala Gly Thr Gly Thr Val Val
145 150 155 160
Val Glu Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala Asp Glu
165 170 175
Thr Arg Val Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
180 185 190
Ala Arg Thr Ala Glu Arg Leu Ala
195 200
<210> 27
<211> 215
<212> PRT
<213> 葡萄(Vitis vinifera)
<220>
<223> 酿酒葡萄PN40024未命名蛋白质产物, 基因座
标签GSVIVT00037390001, GenBank登录号CAO48777.1
<400> 27
Met Pro Ser Asn Pro Pro Lys Ser Ser Leu Val Val His Arg Ile Asn
1 5 10 15
Ser Pro Asn Ser Ile Thr Thr Ala Thr Thr Ala Ser Ala Ala Ala Asn
20 25 30
Asn His Asn Thr Ser Thr Met Pro Pro His Lys Gln Val Pro Asp Ala
35 40 45
Val Ser Arg His His Thr His Val Val Gly Pro Asn Gln Cys Cys Ser
50 55 60
Ala Val Val Gln Gln Ile Ala Ala Pro Val Ser Thr Val Trp Ser Val
65 70 75 80
Val Arg Arg Phe Asp Asn Pro Gln Ala Tyr Lys His Phe Val Lys Ser
85 90 95
Cys His Val Val Val Gly Asp Gly Asp Val Gly Thr Leu Arg Glu Val
100 105 110
His Val Ile Ser Gly Leu Pro Ala Ala Asn Ser Thr Glu Arg Leu Glu
115 120 125
Ile Leu Asp Asp Glu Arg His Val Leu Ser Phe Ser Val Ile Gly Gly
130 135 140
Asp His Arg Leu Ser Asn Tyr Arg Ser Val Thr Thr Leu His Pro Ser
145 150 155 160
Pro Ser Ser Thr Gly Thr Val Val Leu Glu Ser Tyr Val Val Asp Ile
165 170 175
Pro Pro Gly Asn Thr Lys Glu Asp Thr Cys Val Phe Val Asp Thr Ile
180 185 190
Val Arg Cys Asn Leu Gln Ser Leu Ala Gln Ile Ala Glu Asn Ala Ala
195 200 205
Gly Cys Lys Arg Ser Ser Ser
210 215
<210> 28
<211> 213
<212> PRT
<213> 烟草(Nicotiana tabacum)
<220>
<223> 烟草假设蛋白, 基因c17, GenBank
登录号CAI84653.1
<400> 28
Met Pro Pro Ser Ser Pro Asp Ser Ser Val Leu Leu Gln Arg Ile Ser
1 5 10 15
Ser Asn Thr Thr Pro Asp Phe Ala Cys Lys Gln Ser Gln Gln Leu Gln
20 25 30
Arg Arg Thr Met Pro Ile Pro Cys Thr Thr Gln Val Pro Asp Ser Val
35 40 45
Val Arg Phe His Thr His Pro Val Gly Pro Asn Gln Cys Cys Ser Ala
50 55 60
Val Ile Gln Arg Ile Ser Ala Pro Val Ser Thr Val Trp Ser Val Val
65 70 75 80
Arg Arg Phe Asp Asn Pro Gln Ala Tyr Lys His Phe Val Lys Ser Cys
85 90 95
His Val Ile Val Gly Asp Gly Asp Val Gly Thr Leu Arg Glu Val Arg
100 105 110
Val Ile Ser Gly Leu Pro Ala Ala Ser Ser Thr Glu Arg Leu Glu Ile
115 120 125
Leu Asp Asp Glu Arg His Val Ile Ser Phe Ser Val Val Gly Gly Asp
130 135 140
His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr Leu His Pro Glu Pro
145 150 155 160
Ser Gly Asp Gly Thr Thr Ile Val Val Glu Ser Tyr Val Val Asp Val
165 170 175
Pro Pro Gly Asn Thr Arg Asp Glu Thr Cys Val Phe Val Asp Thr Ile
180 185 190
Val Lys Cys Asn Leu Thr Ser Leu Ser Gln Ile Ala Val Asn Val Asn
195 200 205
Arg Arg Lys Asp Ser
210
<210> 29
<211> 208
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻籼组, 品种93-11, 假设蛋白
OsI_04285, GenBank登录号EAY76350.1
<400> 29
Met Pro Tyr Ala Ala Val Arg Pro Ser Pro Pro Pro Gln Leu Ser Arg
1 5 10 15
Pro Ile Gly Ser Gly Ala Gly Gly Gly Lys Ala Cys Pro Ala Val Pro
20 25 30
Cys Glu Val Ala Arg Tyr His Glu His Ala Val Gly Ala Gly Gln Cys
35 40 45
Cys Ser Thr Val Val Gln Ala Ile Ala Ala Pro Ala Asp Ala Val Trp
50 55 60
Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys Lys Phe Ile
65 70 75 80
Lys Ser Cys Arg Leu Val Asp Gly Asp Gly Gly Glu Val Gly Ser Val
85 90 95
Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Thr Ser Ser Arg Glu
100 105 110
Arg Leu Glu Val Leu Asp Asp Asp Arg Arg Val Leu Ser Phe Arg Ile
115 120 125
Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr Val
130 135 140
His Glu Ala Ala Ala Pro Ala Met Ala Val Val Val Glu Ser Tyr Val
145 150 155 160
Val Asp Val Pro Pro Gly Asn Thr Trp Glu Glu Thr Arg Val Phe Val
165 170 175
Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Ala Arg Thr Val Glu
180 185 190
Arg Leu Ala Pro Glu Ala Pro Arg Ala Asn Gly Ser Ile Asp His Ala
195 200 205
<210> 30
<211> 208
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻粳组, 日本晴品种, Bet v I
过敏原样蛋白, 基因B1088C09.11, 克隆 B1088C09,
GenBank登录号BAB68102.1
<400> 30
Met Pro Tyr Ala Ala Val Arg Pro Ser Pro Pro Pro Gln Leu Ser Arg
1 5 10 15
Pro Ile Gly Ser Gly Ala Gly Gly Gly Lys Ala Cys Pro Ala Val Pro
20 25 30
Cys Glu Val Ala Arg Tyr His Glu His Ala Val Gly Ala Gly Gln Cys
35 40 45
Phe Ser Thr Val Val Gln Ala Ile Ala Ala Pro Ala Asp Ala Val Trp
50 55 60
Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys Lys Phe Ile
65 70 75 80
Lys Ser Cys Arg Leu Val Asp Gly Asp Gly Gly Glu Val Gly Ser Val
85 90 95
Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Thr Ser Ser Arg Glu
100 105 110
Arg Leu Glu Val Leu Asp Asp Asp Arg Arg Val Leu Ser Phe Arg Ile
115 120 125
Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr Val
130 135 140
His Glu Ala Ala Ala Pro Ala Met Ala Val Val Val Glu Ser Tyr Val
145 150 155 160
Val Asp Val Pro Pro Gly Asn Thr Trp Glu Glu Thr Arg Val Phe Val
165 170 175
Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Ala Arg Thr Val Glu
180 185 190
Arg Leu Ala Pro Glu Ala Pro Arg Ala Asn Gly Ser Ile Asp His Ala
195 200 205
<210> 31
<211> 213
<212> PRT
<213> 北美云杉(Picea sitchensis)
<220>
<223> 西加云杉(Sitka spruce)品种FB3-425, 未知蛋白,
克隆 WS0276_P02, GenBank登录号ABK22940.1
<400> 31
Met Asp Ile Ile Ala Gly Phe Asp Gln Leu Ser Phe Arg Leu Ser Gly
1 5 10 15
Ala Ser Lys Gln Ile Thr Lys Thr Gly Ala Val Gln Tyr Leu Lys Gly
20 25 30
Glu Glu Gly Tyr Gly Glu Trp Leu Lys Glu Val Met Gly Arg Tyr His
35 40 45
Tyr His Ser His Asp Gly Ala Arg Glu Cys Arg Cys Ser Ser Val Val
50 55 60
Val Gln Gln Val Glu Ala Pro Val Ser Val Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Val Tyr Lys His Phe Val Ser Asn Cys Phe
85 90 95
Met Arg Gly Asp Leu Lys Val Gly Cys Leu Arg Glu Val Arg Val Val
100 105 110
Ser Gly Leu Pro Ala Ala Thr Ser Thr Glu Arg Leu Asp Ile Leu Asp
115 120 125
Glu Glu Arg His Ile Leu Ser Phe Ser Ile Val Gly Gly Asp His Arg
130 135 140
Leu Asn Asn Tyr Arg Ser Ile Thr Thr Leu His Glu Thr Leu Ile Asn
145 150 155 160
Gly Lys Pro Gly Thr Ile Val Ile Glu Ser Tyr Val Leu Asp Val Pro
165 170 175
His Gly Asn Thr Lys Glu Glu Thr Cys Leu Phe Val Asp Thr Ile Val
180 185 190
Lys Cys Asn Leu Gln Ser Leu Ala His Val Ser Asn His Leu Asn Ser
195 200 205
Thr His Arg Cys Leu
210
<210> 32
<211> 207
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻粳组, 日本晴品种, 假设
蛋白Os06g0562200, Bet v I 过敏原家族蛋白,
GenBank登录号NP_001057874.1
<400> 32
Met Glu Ala His Val Glu Arg Ala Leu Arg Glu Gly Leu Thr Glu Glu
1 5 10 15
Glu Arg Ala Ala Leu Glu Pro Ala Val Met Ala His His Thr Phe Pro
20 25 30
Pro Ser Thr Thr Thr Ala Thr Thr Ala Ala Ala Thr Cys Thr Ser Leu
35 40 45
Val Thr Gln Arg Val Ala Ala Pro Val Arg Ala Val Trp Pro Ile Val
50 55 60
Arg Ser Phe Gly Asn Pro Gln Arg Tyr Lys His Phe Val Arg Thr Cys
65 70 75 80
Ala Leu Ala Ala Gly Asp Gly Ala Ser Val Gly Ser Val Arg Glu Val
85 90 95
Thr Val Val Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu
100 105 110
Met Leu Asp Asp Asp Arg His Ile Ile Ser Phe Arg Val Val Gly Gly
115 120 125
Gln His Arg Leu Arg Asn Tyr Arg Ser Val Thr Ser Val Thr Glu Phe
130 135 140
Gln Pro Pro Ala Ala Gly Pro Gly Pro Ala Pro Pro Tyr Cys Val Val
145 150 155 160
Val Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Ala Glu Asp
165 170 175
Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln Met Leu
180 185 190
Ala Ala Val Ala Glu Asp Ser Ser Ser Ala Ser Arg Arg Arg Asp
195 200 205
<210> 33
<211> 216
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻粳组, 日本晴品种, 假设
蛋白Os05g0473000, 链霉菌环化酶/脱氢酶家族
蛋白, GenBank登录号NP_001055819.1
<400> 33
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Pro Gln His Ser Arg Ile
1 5 10 15
Gly Gly Cys Gly Gly Gly Gly Val Leu Lys Ala Ala Gly Ala Ala Gly
20 25 30
His Ala Ala Ser Cys Val Ala Val Pro Ala Glu Val Ala Arg His His
35 40 45
Glu His Ala Ala Gly Val Gly Gln Cys Cys Ser Ala Val Val Gln Ala
50 55 60
Ile Ala Ala Pro Val Asp Ala Val Trp Ser Val Val Arg Arg Phe Asp
65 70 75 80
Arg Pro Gln Ala Tyr Lys His Phe Ile Arg Ser Cys Arg Leu Leu Asp
85 90 95
Gly Asp Gly Asp Gly Gly Ala Val Ala Val Gly Ser Val Arg Glu Val
100 105 110
Arg Val Val Ser Gly Leu Pro Ala Thr Ser Ser Arg Glu Arg Leu Glu
115 120 125
Ile Leu Asp Asp Glu Arg Arg Val Leu Ser Phe Arg Val Val Gly Gly
130 135 140
Glu His Arg Leu Ser Asn Tyr Arg Ser Val Thr Thr Val His Glu Thr
145 150 155 160
Ala Ala Gly Ala Ala Ala Ala Val Val Val Glu Ser Tyr Val Val Asp
165 170 175
Val Pro His Gly Asn Thr Ala Asp Glu Thr Arg Met Phe Val Asp Thr
180 185 190
Ile Val Arg Cys Asn Leu Gln Ser Leu Ala Arg Thr Ala Glu Gln Leu
195 200 205
Ala Leu Ala Ala Pro Arg Ala Ala
210 215
<210> 34
<211> 212
<212> PRT
<213> 葡萄(Vitis vinifera)
<220>
<223> 酿酒葡萄PN40024未命名蛋白质产物, 基因座
标签GSVIVT00029365001, GenBank登录号CAO41436.1
<400> 34
Met Pro Ser Ser Leu Gln Leu His Arg Ile Asn Asn Ile Asp Pro Thr
1 5 10 15
Thr Val Ala Val Ala Ala Thr Ala Ala Val Asn Cys His Lys Gln Ser
20 25 30
Arg Thr Pro Leu Arg Cys Ala Thr Pro Val Pro Asp Ala Val Ala Ser
35 40 45
Tyr His Ala His Ala Val Gly Pro His Gln Cys Cys Ser Met Val Val
50 55 60
Gln Thr Thr Ala Ala Ala Leu Pro Thr Val Trp Ser Val Val Arg Arg
65 70 75 80
Phe Asp Asn Pro Gln Ala Tyr Lys His Phe Leu Lys Ser Cys His Val
85 90 95
Ile Phe Gly Asp Gly Asp Ile Gly Thr Leu Arg Glu Val His Val Val
100 105 110
Ser Gly Leu Pro Ala Glu Ser Ser Thr Glu Arg Leu Glu Ile Leu Asp
115 120 125
Asp Glu Arg His Val Leu Ser Phe Ser Val Val Gly Gly Asp His Arg
130 135 140
Leu Cys Asn Tyr Arg Ser Val Thr Thr Leu His Pro Ser Pro Thr Gly
145 150 155 160
Thr Gly Thr Val Val Val Glu Ser Tyr Val Val Asp Ile Pro Pro Gly
165 170 175
Asn Thr Lys Glu Asp Thr Cys Val Phe Val Asp Thr Ile Val Lys Cys
180 185 190
Asn Leu Gln Ser Leu Ala Gln Met Ser Glu Lys Leu Thr Asn Asn Asn
195 200 205
Arg Asn Ser Ser
210
<210> 35
<211> 218
<212> PRT
<213> 玉米(Zea mays)
<220>
<223> 玉米环化酶/脱氢酶家族蛋白, 克隆 1678999,
GenBank登录号ACG30334.1
<400> 35
Met Pro Cys Leu Gln Ala Ser Ser Pro Gly Ser Met Pro Tyr Gln His
1 5 10 15
His Gly Arg Gly Val Gly Cys Ala Ala Glu Ala Gly Ala Ala Val Gly
20 25 30
Ala Ser Ala Gly Thr Gly Thr Arg Cys Gly Ala His Asp Gly Glu Val
35 40 45
Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Pro Gly Pro Gly
50 55 60
Arg Cys Cys Ser Ala Val Val Gln Arg Val Ala Ala Pro Ala Glu Ala
65 70 75 80
Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Ala Tyr Lys Arg
85 90 95
Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly Val Gly Thr
100 105 110
Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Ala Ser Ser Arg
115 120 125
Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu Ser Phe Arg
130 135 140
Val Val Gly Gly Glu His Arg Leu Gln Asn Tyr Leu Ser Val Thr Thr
145 150 155 160
Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val Val Val Glu
165 170 175
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu Asp Thr Arg
180 185 190
Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln Ser Leu Ala Thr
195 200 205
Thr Ala Glu Lys Leu Ala Leu Ala Ala Val
210 215
<210> 36
<211> 179
<212> PRT
<213> 小立碗藓(Physcomitrella patens)
<220>
<223> 小立碗藓(Physcomitrella patens)亚种. 金属盘苔藓, Gransden生态型
2004, 假设蛋白, 预计蛋白, 基因座标签
PHYPADRAFT_222359, GenBank登录号XP_001778048.1
<400> 36
Met Gln Thr Lys Gly Arg Gln Ala Asp Phe Gln Thr Leu Leu Glu Gly
1 5 10 15
Gln Gln Asp Leu Ile Cys Arg Phe His Arg His Glu Leu Gln Pro His
20 25 30
Gln Cys Gly Ser Ile Leu Leu Gln Leu Ile Lys Ala Pro Val Glu Thr
35 40 45
Val Trp Ser Val Ala Arg Ser Phe Asp Lys Pro Gln Val Tyr Lys Arg
50 55 60
Phe Ile Gln Thr Cys Glu Ile Ile Glu Gly Asp Gly Gly Val Gly Ser
65 70 75 80
Ile Arg Glu Val Arg Leu Val Ser Ser Ile Pro Ala Thr Ser Ser Ile
85 90 95
Glu Arg Leu Glu Ile Leu Asp Asp Glu Glu His Ile Ile Ser Phe Arg
100 105 110
Val Leu Gly Gly Gly His Arg Leu Gln Asn Tyr Trp Ser Val Thr Ser
115 120 125
Leu His Ser His Glu Ile Asp Gly Gln Met Gly Thr Leu Val Leu Glu
130 135 140
Ser Tyr Val Val Asp Ile Pro Glu Gly Asn Thr Arg Glu Glu Thr His
145 150 155 160
Met Phe Val Asp Thr Val Val Arg Cys Asn Leu Lys Ala Leu Ala Gln
165 170 175
Val Ser Glu
<210> 37
<211> 229
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻籼组, 品种93-11, 假设蛋白
OsI_11160, GenBank登录号EAY89631.1
<400> 37
Met Pro Cys Ile Pro Ala Ser Ser Pro Gly Ile Pro His Gln His Gln
1 5 10 15
His Gln His His Arg Ala Leu Ala Gly Val Gly Met Ala Val Gly Cys
20 25 30
Ala Ala Glu Ala Ala Val Ala Ala Ala Gly Val Ala Gly Thr Arg Cys
35 40 45
Gly Ala His Asp Gly Glu Val Pro Met Glu Val Ala Arg His His Glu
50 55 60
His Ala Glu Pro Gly Ser Gly Arg Cys Cys Ser Ala Val Val Gln His
65 70 75 80
Val Ala Ala Pro Ala Pro Ala Val Trp Ser Val Val Arg Arg Phe Asp
85 90 95
Gln Pro Gln Ala Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala
100 105 110
Gly Asp Gly Gly Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly
115 120 125
Leu Pro Ala Ala Ser Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu
130 135 140
Ser His Val Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Lys
145 150 155 160
Asn Tyr Leu Ser Val Thr Thr Val His Pro Ser Pro Ser Ala Pro Thr
165 170 175
Ala Ala Thr Val Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Pro Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys
195 200 205
Asn Leu Gln Ser Leu Ala Lys Thr Ala Glu Lys Leu Ala Ala Gly Ala
210 215 220
Arg Ala Ala Gly Ser
225
<210> 38
<211> 229
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻粳组, 日本晴品种, 假设
蛋白Os03g0297600, 链霉菌环化酶/脱氢酶家族
蛋白, GenBank登录号NP_001049838.1
<400> 38
Met Pro Cys Ile Pro Ala Ser Ser Pro Gly Ile Pro His Gln His Gln
1 5 10 15
His Gln His His Arg Ala Leu Ala Gly Val Gly Met Ala Val Gly Cys
20 25 30
Ala Ala Glu Ala Ala Val Ala Ala Ala Gly Val Ala Gly Thr Arg Cys
35 40 45
Gly Ala His Asp Gly Glu Val Pro Met Glu Val Ala Arg His His Glu
50 55 60
His Ala Glu Pro Gly Ser Gly Arg Cys Cys Ser Ala Val Val Gln His
65 70 75 80
Val Ala Ala Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp
85 90 95
Gln Pro Gln Ala Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala
100 105 110
Gly Asp Gly Gly Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly
115 120 125
Leu Pro Ala Ala Ser Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu
130 135 140
Ser His Val Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Lys
145 150 155 160
Asn Tyr Leu Ser Val Thr Thr Val His Pro Ser Pro Ser Ala Pro Thr
165 170 175
Ala Ala Thr Val Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Pro Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys
195 200 205
Asn Leu Gln Ser Leu Ala Lys Thr Ala Glu Lys Leu Ala Ala Gly Ala
210 215 220
Arg Ala Ala Gly Ser
225
<210> 39
<211> 205
<212> PRT
<213> 蒺藜状苜蓿(Medicago truncatula)
<220>
<223> 蒺藜状苜蓿未知蛋白, 克隆 MTYFP_FQ_FR_FS1G-H-19,
GenBank登录号ACJ85898.1
<400> 39
Met Pro Ser Pro Val Gln Phe Gln Arg Phe Asp Ser Asn Thr Ala Ile
1 5 10 15
Thr Asn Gly Val Asn Cys Pro Lys Gln Ile Gln Ala Cys Arg Tyr Ala
20 25 30
Leu Ser Ser Leu Lys Pro Thr Val Ser Val Pro Glu Thr Val Val Asp
35 40 45
His His Met His Val Val Gly Gln Asn Gln Cys Tyr Ser Val Val Ile
50 55 60
Gln Thr Ile Asn Ala Ser Val Ser Thr Val Trp Ser Val Val Arg Arg
65 70 75 80
Phe Asp Tyr Pro Gln Gly Tyr Lys His Phe Val Lys Ser Cys Asn Val
85 90 95
Val Ala Ser Gly Asp Gly Ile Arg Val Gly Ala Leu Arg Glu Val Arg
100 105 110
Leu Val Ser Gly Leu Pro Ala Val Ser Ser Thr Glu Arg Leu Asp Ile
115 120 125
Leu Asp Glu Glu Arg His Val Ile Ser Phe Ser Val Val Gly Gly Val
130 135 140
His Arg Cys Arg Asn Tyr Arg Ser Val Thr Thr Leu His Gly Asp Gly
145 150 155 160
Asn Gly Gly Thr Val Val Ile Glu Ser Tyr Val Val Asp Val Pro Gln
165 170 175
Gly Asn Thr Lys Glu Glu Thr Cys Ser Phe Ala Asp Thr Ile Val Arg
180 185 190
Cys Asn Leu Gln Ser Leu Val Gln Ile Ala Glu Lys Leu
195 200 205
<210> 40
<211> 212
<212> PRT
<213> 玉米(Zea mays)
<220>
<223> 玉米AT-富含型元件结合因子3, 克隆 1458362,
GenBank登录号ACG26321.1
<400> 40
Met Pro Phe Ala Ala Ser Arg Thr Ser Gln Gln Gln His Ser Arg Val
1 5 10 15
Ala Thr Asn Gly Arg Ala Val Ala Val Cys Ala Gly His Ala Gly Val
20 25 30
Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala Ala Gly Gln
35 40 45
Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala Pro Val Asp Ala Val
50 55 60
Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Arg Tyr Lys Arg Phe
65 70 75 80
Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Ala Glu Val Gly Ser
85 90 95
Val Arg Glu Leu Leu Leu Val Ser Gly Leu Pro Ala Glu Ser Ser Arg
100 105 110
Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile Ser Phe Arg
115 120 125
Val Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr
130 135 140
Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg Pro Leu Thr Met Val
145 150 155 160
Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Val Glu Glu
165 170 175
Thr Arg Ile Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
180 185 190
Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala Ala Met Pro His Asp
195 200 205
Asp Asn Gln Asn
210
<210> 41
<211> 233
<212> PRT
<213> 玉米(Zea mays)
<220>
<223> 玉米株B73 未知蛋白, 克隆 ZM_BFb0105O18,
GenBank登录号ACF87013.1
<400> 41
Met Arg Glu Arg Asn Ser Ser Ile Asp Gln Glu His Gln Arg Gly Ser
1 5 10 15
Ser Ser Arg Ser Thr Met Pro Phe Ala Ala Ser Arg Thr Ser Gln Gln
20 25 30
Gln His Ser Arg Val Ala Thr Asn Gly Arg Ala Val Ala Val Cys Ala
35 40 45
Gly His Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala
50 55 60
Val Ala Ala Gly Gln Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala
65 70 75 80
Pro Val Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Arg Tyr Lys Arg Phe Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly
100 105 110
Ala Glu Val Gly Ser Val Arg Glu Leu Leu Leu Val Ser Gly Leu Pro
115 120 125
Ala Glu Ser Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg
130 135 140
Val Ile Ser Phe Arg Val Leu Gly Gly Asp His Arg Leu Ala Asn Tyr
145 150 155 160
Arg Ser Val Thr Thr Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg
165 170 175
Pro Leu Thr Met Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Val Glu Glu Thr Arg Ile Phe Val Asp Thr Ile Val Arg Cys
195 200 205
Asn Leu Gln Ser Leu Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala
210 215 220
Ala Met Pro His Asp Asp Asn Gln Asn
225 230
<210> 42
<211> 194
<212> PRT
<213> 小立碗藓(Physcomitrella patens)
<220>
<223> 小立碗藓(Physcomitrella patens)亚种. 金属盘苔藓, Gransden生态型
2004, 假设蛋白, 预计蛋白, 基因座标签
PHYPADRAFT_209242, GenBank登录号XP_001762113.1
<400> 42
Met Met Gln Glu Lys Gln Gly Arg Pro Asp Phe Gln Phe Leu Leu Glu
1 5 10 15
Gly Gln Gln Asp Leu Ile Cys Arg Phe His Lys His Glu Leu Leu Pro
20 25 30
His Gln Cys Gly Ser Ile Leu Leu Gln Gln Ile Lys Ala Pro Val Gln
35 40 45
Thr Val Trp Leu Ile Val Arg Arg Phe Asp Glu Pro Gln Val Tyr Lys
50 55 60
Arg Phe Ile Gln Arg Cys Asp Ile Val Glu Gly Asp Gly Val Val Gly
65 70 75 80
Ser Ile Arg Glu Val Gln Leu Val Ser Ser Ile Pro Ala Thr Ser Ser
85 90 95
Ile Glu Arg Leu Glu Ile Leu Asp Asp Glu Glu His Ile Ile Ser Phe
100 105 110
Arg Val Leu Gly Gly Gly His Arg Leu Gln Asn Tyr Trp Ser Val Thr
115 120 125
Ser Leu His Arg His Glu Ile Gln Gly Gln Met Gly Thr Leu Val Leu
130 135 140
Glu Ser Tyr Val Val Asp Ile Pro Asp Gly Asn Thr Arg Glu Glu Thr
145 150 155 160
His Thr Phe Val Asp Thr Val Val Arg Cys Asn Leu Lys Ala Leu Ala
165 170 175
Gln Val Ser Glu Gln Lys His Leu Leu Asn Ser Asn Glu Lys Pro Ala
180 185 190
Ala Pro
<210> 43
<211> 191
<212> PRT
<213> 葡萄(Vitis vinifera)
<220>
<223> 酿酒葡萄PN40024未命名蛋白质产物, 基因座
标签GSVIVT00035869001, GenBank登录号CAO48052.1
<400> 43
Met Lys Val Tyr Ser Pro Ser Gln Ile Leu Ala Glu Arg Gly Pro Arg
1 5 10 15
Ala Gln Ala Met Gly Asn Leu Tyr His Thr His His Leu Leu Pro Asn
20 25 30
Gln Cys Ser Ser Leu Val Val Gln Thr Thr Asp Ala Pro Leu Pro Gln
35 40 45
Val Trp Ser Met Val Arg Arg Phe Asp Arg Pro Gln Ser Tyr Lys Arg
50 55 60
Phe Val Arg Gly Cys Thr Leu Arg Arg Gly Lys Gly Gly Val Gly Ser
65 70 75 80
Val Arg Glu Val Asn Ile Val Ser Gly Leu Pro Ala Glu Ile Ser Leu
85 90 95
Glu Arg Leu Asp Lys Leu Asp Asp Asp Leu His Val Met Arg Phe Thr
100 105 110
Val Ile Gly Gly Asp His Arg Leu Ala Asn Tyr His Ser Thr Leu Thr
115 120 125
Leu His Glu Asp Glu Glu Asp Gly Val Arg Lys Thr Val Val Met Glu
130 135 140
Ser Tyr Val Val Asp Val Pro Gly Gly Asn Ser Ala Gly Glu Thr Cys
145 150 155 160
Tyr Phe Ala Asn Thr Ile Ile Gly Phe Asn Leu Lys Ala Leu Ala Ala
165 170 175
Val Thr Glu Thr Met Ala Leu Lys Ala Asn Ile Pro Ser Gly Phe
180 185 190
<210> 44
<211> 217
<212> PRT
<213> 小立碗藓(Physcomitrella patens)
<220>
<223> 小立碗藓(Physcomitrella patens)亚种. 金属盘苔藓, Gransden生态型
2004, 假设蛋白, 预计蛋白, 基因座标签
PHYPADRAFT_132509, GenBank登录号XP_001767821.1
<400> 44
Met Gln Gln Val Lys Gly Arg Gln Asp Phe Gln Arg Leu Leu Glu Ala
1 5 10 15
Gln Gln Asp Leu Ile Cys Arg Tyr His Thr His Glu Leu Lys Ala His
20 25 30
Gln Cys Gly Ser Ile Leu Leu Gln Gln Ile Lys Val Pro Leu Pro Ile
35 40 45
Val Trp Ala Ile Val Arg Ser Phe Asp Lys Pro Gln Val Tyr Lys Arg
50 55 60
Phe Ile Gln Thr Cys Lys Ile Thr Glu Gly Asp Gly Gly Val Gly Ser
65 70 75 80
Ile Arg Glu Val His Leu Val Ser Ser Val Pro Ala Thr Cys Ser Ile
85 90 95
Glu Arg Leu Glu Ile Leu Asp Asp Glu Lys His Ile Ile Ser Phe Arg
100 105 110
Val Leu Gly Gly Gly His Arg Leu Gln Asn Tyr Ser Ser Val Ser Ser
115 120 125
Leu His Glu Leu Glu Val Glu Gly His Pro Cys Thr Leu Val Leu Glu
130 135 140
Ser Tyr Met Val Asp Ile Pro Asp Gly Asn Thr Arg Glu Glu Thr His
145 150 155 160
Met Phe Val Asp Thr Val Val Arg Cys Asn Leu Lys Ser Leu Ala Gln
165 170 175
Ile Ser Glu Gln Gln Tyr Asn Lys Asp Cys Leu Gln Gln Lys Gln His
180 185 190
Asp Gln Gln Gln Met Tyr Gln Gln Arg His Pro Pro Leu Pro Pro Ile
195 200 205
Pro Ile Thr Asp Lys Asn Met Glu Arg
210 215
<210> 45
<211> 195
<212> PRT
<213> 小立碗藓(Physcomitrella patens)
<220>
<223> 小立碗藓(Physcomitrella patens)亚种. 金属盘苔藓, Gransden生态型
2004, 假设蛋白, 预计蛋白, 基因座标签
PHYPADRAFT_213389, GenBank登录号XP_001767012.1
<400> 45
Met Arg Phe Asp Ile Gly His Asn Asp Val Arg Gly Phe Phe Thr Cys
1 5 10 15
Glu Glu Glu His Ala Tyr Ala Leu His Ser Gln Thr Val Glu Leu Asn
20 25 30
Gln Cys Gly Ser Ile Leu Met Gln Gln Ile His Ala Pro Ile Glu Val
35 40 45
Val Trp Ser Ile Val Arg Ser Phe Gly Ser Pro Gln Ile Tyr Lys Lys
50 55 60
Phe Ile Gln Ala Cys Ile Leu Thr Val Gly Asp Gly Gly Val Gly Ser
65 70 75 80
Ile Arg Glu Val Phe Leu Val Ser Gly Val Pro Ala Thr Ser Ser Ile
85 90 95
Glu Arg Leu Glu Ile Leu Asp Asp Glu Lys His Val Phe Ser Phe Arg
100 105 110
Val Leu Lys Gly Gly His Arg Leu Gln Asn Tyr Arg Ser Val Thr Thr
115 120 125
Leu His Glu Gln Glu Val Asn Gly Arg Gln Thr Thr Thr Val Leu Glu
130 135 140
Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Arg Glu Glu Thr His
145 150 155 160
Met Phe Ala Asp Thr Val Val Met Cys Asn Leu Lys Ser Leu Ala Gln
165 170 175
Val Ala Glu Trp Arg Ala Met Gln Gly Ile Thr Gln Gln Leu Ser Thr
180 185 190
Ser Ser Leu
195
<210> 46
<211> 172
<212> PRT
<213> 葡萄(Vitis vinifera)
<220>
<223> 酿酒葡萄黑皮诺假设蛋白, 克隆
ENTAV 115, 基因座标签VITISV_004947, GenBank登录号
CAN72620.1
<400> 46
Met Gly Asn Leu Tyr His Thr His His Leu Leu Pro Asn Gln Cys Ser
1 5 10 15
Ser Leu Val Val Gln Thr Thr Asp Ala Pro Leu Pro Gln Val Trp Ser
20 25 30
Met Val Arg Arg Phe Asp Arg Pro Gln Ser Tyr Lys Arg Phe Val Arg
35 40 45
Gly Cys Thr Leu Arg Arg Gly Lys Gly Gly Val Gly Ser Val Arg Glu
50 55 60
Val Asn Ile Val Ser Gly Leu Pro Ala Glu Ile Ser Leu Glu Arg Leu
65 70 75 80
Asp Lys Leu Asp Asp Asp Leu His Val Met Arg Phe Thr Val Ile Gly
85 90 95
Gly Asp His Arg Leu Ala Asn Tyr His Ser Thr Leu Thr Leu His Glu
100 105 110
Asp Glu Glu Asp Gly Val Arg Lys Thr Val Val Met Glu Ser Tyr Val
115 120 125
Val Asp Val Pro Gly Gly Asn Ser Ala Gly Glu Thr Cys Tyr Phe Ala
130 135 140
Asn Thr Ile Ile Gly Phe Asn Leu Lys Ala Leu Ala Ala Val Thr Glu
145 150 155 160
Thr Met Ala Leu Lys Ala Asn Ile Pro Ser Gly Phe
165 170
<210> 47
<211> 196
<212> PRT
<213> 北美云杉(Picea sitchensis)
<220>
<223> 西加云杉(Sitka spruce)品种FB3-425, 未知蛋白,
克隆 WS0281_I24, GenBank登录号ABK23752.1
<400> 47
Met Glu Asp Leu Ser Ser Trp Arg Glu Gly Arg Ala Met Trp Leu Gly
1 5 10 15
Asn Pro Pro Ser Glu Ser Glu Leu Val Cys Arg His His Arg His Glu
20 25 30
Leu Gln Gly Asn Gln Cys Ser Ser Phe Leu Val Lys His Ile Arg Ala
35 40 45
Pro Val His Leu Val Trp Ser Ile Val Arg Thr Phe Asp Gln Pro Gln
50 55 60
Lys Tyr Lys Pro Phe Val His Ser Cys Ser Val Arg Gly Gly Ile Thr
65 70 75 80
Val Gly Ser Ile Arg Asn Val Asn Val Lys Ser Gly Leu Pro Ala Thr
85 90 95
Ala Ser Glu Glu Arg Leu Glu Ile Leu Asp Asp Asn Glu His Val Phe
100 105 110
Ser Ile Lys Ile Leu Gly Gly Asp His Arg Leu Gln Asn Tyr Ser Ser
115 120 125
Ile Ile Thr Val His Pro Glu Ile Ile Asp Gly Arg Pro Gly Thr Leu
130 135 140
Val Ile Glu Ser Tyr Val Val Asp Val Pro Glu Gly Asn Thr Arg Glu
145 150 155 160
Glu Thr Arg Phe Phe Val Glu Ala Leu Val Lys Cys Asn Leu Lys Ser
165 170 175
Leu Ala Asp Val Ser Glu Arg Leu Ala Ser Gln His His Thr Glu Leu
180 185 190
Leu Glu Arg Thr
195
<210> 48
<211> 185
<212> PRT
<213> 马铃薯(Solanum tuberosum)
<220>
<223> 马铃薯品种Kuras, CAPIP1样蛋白, 克隆 153D02,
类似于甜辣椒CAPIP1, GenBank登录号
ABB29920.1
<400> 48
Met Asn Ala Asn Gly Phe Cys Gly Val Glu Lys Glu Tyr Ile Arg Lys
1 5 10 15
His His Leu His Glu Pro Lys Glu Asn Gln Cys Ser Ser Phe Leu Val
20 25 30
Lys His Ile Arg Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg
35 40 45
Phe Asp Gln Pro Gln Lys Tyr Lys Pro Phe Ile Ser Arg Cys Ile Val
50 55 60
Gln Gly Asp Leu Glu Ile Gly Ser Leu Arg Glu Val Asp Val Lys Ser
65 70 75 80
Gly Leu Pro Ala Thr Thr Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp
85 90 95
Glu Glu His Ile Leu Ser Val Arg Ile Val Gly Gly Asp His Arg Leu
100 105 110
Arg Asn Tyr Ser Ser Val Ile Ser Val His Pro Glu Val Ile Asp Gly
115 120 125
Arg Pro Gly Thr Val Val Leu Glu Ser Phe Val Val Asp Val Pro Glu
130 135 140
Gly Asn Thr Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Leu Ile Asn
145 150 155 160
Cys Asn Leu Lys Ser Leu Ala Asp Ile Ser Glu Arg Val Ala Val Gln
165 170 175
Asp Arg Thr Glu Pro Ile Asp Gln Val
180 185
<210> 49
<211> 190
<212> PRT
<213> 蒺藜状苜蓿(Medicago truncatula)
<220>
<223> 蒺藜状苜蓿未知蛋白, 克隆 MTYFP_FQ_FR_FS1G-E-17,
GenBank登录号ACJ85952.1
<400> 49
Met Asn Asn Gly Cys Glu Gln Gln Gln Tyr Ser Val Ile Glu Thr Gln
1 5 10 15
Tyr Ile Arg Arg His His Lys His Asp Leu Arg Asp Asn Gln Cys Ser
20 25 30
Ser Ala Leu Val Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser
35 40 45
Leu Val Arg Arg Phe Asp Gln Pro Gln Lys Tyr Lys Pro Phe Ile Ser
50 55 60
Arg Cys Ile Met Gln Gly Asp Leu Ser Ile Gly Ser Val Arg Glu Val
65 70 75 80
Asn Val Lys Ser Gly Leu Pro Ala Thr Thr Ser Thr Glu Arg Leu Glu
85 90 95
Gln Leu Asp Asp Glu Glu His Ile Leu Gly Ile Arg Ile Val Gly Gly
100 105 110
Asp His Arg Leu Arg Asn Tyr Ser Ser Ile Ile Thr Val His Pro Gly
115 120 125
Val Ile Asp Gly Arg Pro Gly Thr Met Val Ile Glu Ser Phe Val Val
130 135 140
Asp Val Pro Glu Gly Asn Thr Lys Asp Glu Thr Cys Tyr Phe Val Glu
145 150 155 160
Ala Leu Ile Arg Tyr Asn Leu Ser Ser Leu Ala Asp Val Ser Glu Arg
165 170 175
Met Ala Val Gln Gly Arg Thr Asp Pro Ile Asn Ile Asn Pro
180 185 190
<210> 50
<211> 185
<212> PRT
<213> 葡萄(Vitis vinifera)
<220>
<223> 酿酒葡萄PN40024未命名蛋白质产物, 基因座
标签GSVIVT00002440001, GenBank登录号CAO65816.1
<400> 50
Met Ser Gly Tyr Gly Cys Ile Lys Met Glu Asp Glu Tyr Ile Arg Arg
1 5 10 15
His His Arg His Glu Ile Arg Asp Asn Gln Cys Ser Ser Ser Leu Val
20 25 30
Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg Ser
35 40 45
Phe Asp Gln Pro Gln Lys Tyr Lys Pro Phe Val Ser Arg Cys Ile Val
50 55 60
Gln Gly Asp Leu Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys Ser
65 70 75 80
Gly Leu Pro Ala Thr Thr Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp
85 90 95
Glu Glu His Ile Phe Gly Met Arg Ile Val Gly Gly Asp His Arg Leu
100 105 110
Lys Asn Tyr Ser Ser Ile Val Thr Val His Pro Glu Ile Ile Asp Gly
115 120 125
Arg Pro Gly Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp
130 135 140
Gly Asn Thr Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Leu Ile Lys
145 150 155 160
Cys Asn Leu Lys Ser Leu Ala Asp Val Ser Glu Arg Leu Ala Ile Gln
165 170 175
Asp Arg Thr Glu Pro Ile Asp Arg Met
180 185
<210> 51
<211> 185
<212> PRT
<213> 葡萄(Vitis vinifera)
<220>
<223> 酿酒葡萄PN40024未命名蛋白质产物, 基因座
标签GSVIVT00006507001, GenBank登录号CAO69376.1
<400> 51
Met Asn Gly Asn Gly Leu Ser Ser Met Glu Ser Glu Tyr Ile Arg Arg
1 5 10 15
His His Arg His Glu Pro Ala Glu Asn Gln Cys Ser Ser Ala Leu Val
20 25 30
Lys His Ile Lys Ala Pro Val Pro Leu Val Trp Ser Leu Val Arg Arg
35 40 45
Phe Asp Gln Pro Gln Lys Tyr Lys Pro Phe Ile Ser Arg Cys Val Val
50 55 60
Gln Gly Asn Leu Glu Ile Gly Ser Leu Arg Glu Val Asp Val Lys Ser
65 70 75 80
Gly Leu Pro Ala Thr Thr Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp
85 90 95
Asp Glu His Ile Leu Ser Met Arg Ile Ile Gly Gly Asp His Arg Leu
100 105 110
Arg Asn Tyr Ser Ser Ile Ile Ser Leu His Pro Glu Ile Ile Asp Gly
115 120 125
Arg Pro Gly Thr Met Val Ile Glu Ser Tyr Val Val Asp Val Pro Glu
130 135 140
Gly Asn Thr Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Leu Ile Lys
145 150 155 160
Cys Asn Leu Lys Ser Leu Ala Asp Val Ser Glu Arg Leu Ala Val Gln
165 170 175
Asp Arg Thr Glu Pro Ile Asp Arg Met
180 185
<210> 52
<211> 208
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻粳组, 日本晴品种, 假设
蛋白OsJ_21703, GenBank登录号EAZ37364.1
<400> 52
Met Glu Ala His Val Glu Arg Ala Leu Arg Glu Gly Leu Thr Glu Glu
1 5 10 15
Glu Arg Ala Ala Leu Glu Pro Ala Val Met Ala His His Thr Phe Pro
20 25 30
Pro Ser Thr Thr Thr Ala Thr Thr Ala Ala Ala Thr Cys Thr Ser Leu
35 40 45
Val Thr Gln Arg Val Ala Ala Pro Val Arg Ala Val Trp Pro Ile Val
50 55 60
Arg Ser Phe Gly Asn Pro Gln Arg Tyr Lys His Phe Val Arg Thr Cys
65 70 75 80
Ala Leu Ala Ala Gly Asn Gly Pro Ser Phe Gly Ser Val Arg Glu Val
85 90 95
Thr Val Val Ser Gly Pro Ser Arg Leu Pro Pro Gly Thr Glu Arg Leu
100 105 110
Glu Met Leu Asp Asp Asp Arg His Ile Ile Ser Phe Arg Val Val Gly
115 120 125
Gly Gln His Arg Leu Arg Asn Tyr Arg Ser Val Thr Ser Val Thr Glu
130 135 140
Phe Gln Pro Pro Ala Ala Gly Pro Gly Pro Ala Pro Pro Tyr Cys Val
145 150 155 160
Val Val Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Ala Glu
165 170 175
Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln Met
180 185 190
Leu Ala Ala Val Ala Glu Asp Ser Ser Ser Ala Ser Arg Arg Arg Asp
195 200 205
<210> 53
<211> 186
<212> PRT
<213> 甜辣椒(Capsicum annuum)
<220>
<223> 胡椒品种Hanbyul, CAPIP1蛋白, GenBank
登录号AAT35532.1
<400> 53
Met Met Asn Ala Asn Gly Phe Ser Gly Val Glu Lys Glu Tyr Ile Arg
1 5 10 15
Lys His His Leu His Gln Pro Lys Glu Asn Gln Cys Ser Ser Phe Leu
20 25 30
Val Lys His Ile Arg Ala Pro Val His Leu Val Trp Ser Leu Val Arg
35 40 45
Arg Phe Asp Gln Pro Gln Lys Tyr Lys Pro Phe Val Ser Arg Cys Ile
50 55 60
Ala Gln Gly Asp Leu Glu Ile Gly Ser Leu Arg Glu Val Asp Val Lys
65 70 75 80
Ser Gly Leu Pro Ala Thr Thr Ser Thr Glu Arg Leu Glu Leu Leu Asp
85 90 95
Asp Glu Glu His Ile Leu Ser Phe Arg Ile Ile Gly Gly Asp His Arg
100 105 110
Leu Arg Asn Tyr Ser Ser Ile Ile Ser Leu His Pro Glu Val Ile Asp
115 120 125
Gly Arg Pro Gly Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro
130 135 140
Gln Gly Asn Thr Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Leu Ile
145 150 155 160
Asn Cys Asn Leu Lys Ser Leu Ala Asp Val Ser Glu Arg Leu Ala Val
165 170 175
Gln Asp Arg Thr Glu Pro Ile Asp Gln Val
180 185
<210> 54
<211> 186
<212> PRT
<213> 毛果杨(Populus trichocarpa)
<220>
<223> 加利福尼亚白杨(西方胶杨,黑杨)
品种383-2499 (Nisqually-1), 未知蛋白, 克隆
PX0011_1113, GenBank登录号ABK92491.1
<400> 54
Met Asn Gly Ser Asp Ala Tyr Ser Ala Thr Glu Ala Gln Tyr Val Arg
1 5 10 15
Arg His His Lys His Glu Pro Arg Glu Asn Gln Cys Thr Ser Ala Leu
20 25 30
Val Lys His Ile Lys Ala Pro Ala His Leu Val Trp Ser Leu Val Arg
35 40 45
Arg Phe Asp Gln Pro Gln Arg Tyr Lys Pro Phe Val Ser Arg Cys Val
50 55 60
Met Asn Gly Glu Leu Gly Ile Gly Ser Val Arg Glu Val Asn Val Lys
65 70 75 80
Ser Gly Leu Pro Ala Thr Thr Ser Thr Glu Arg Leu Glu Leu Leu Asp
85 90 95
Asp Glu Glu His Ile Leu Gly Val Gln Ile Val Gly Gly Asp His Arg
100 105 110
Leu Lys Asn Tyr Ser Ser Ile Met Thr Val His Pro Glu Phe Ile Asp
115 120 125
Gly Arg Pro Gly Thr Leu Val Ile Glu Ser Phe Ile Val Asp Val Pro
130 135 140
Asp Gly Asn Thr Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Leu Ile
145 150 155 160
Arg Cys Asn Leu Lys Ser Leu Ala Asp Val Ser Glu Arg Met Ala Val
165 170 175
Gln Asp Arg Val Glu Pro Val Asn Gln Phe
180 185
<210> 55
<211> 185
<212> PRT
<213> 甜辣椒(Capsicum annuum)
<220>
<223> 胡椒品种Hanbyul, PIP1蛋白, GenBank
登录号ABF72432.1
<400> 55
Met Asn Ala Asn Gly Phe Ser Gly Val Glu Lys Glu Tyr Ile Arg Lys
1 5 10 15
His His Leu His Gln Pro Lys Glu Asn Gln Cys Ser Ser Phe Leu Val
20 25 30
Lys His Ile Arg Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg
35 40 45
Phe Asp Gln Pro Gln Lys Tyr Lys Pro Phe Val Ser Arg Cys Ile Ala
50 55 60
Gln Gly Asp Leu Glu Ile Gly Ser Leu Arg Glu Val Asp Val Lys Ser
65 70 75 80
Gly Leu Pro Ala Thr Thr Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp
85 90 95
Glu Glu His Ile Leu Ser Phe Arg Ile Ile Gly Gly Asp His Arg Leu
100 105 110
Arg Asn Tyr Ser Ser Ile Ile Ser Leu His Pro Glu Val Ile Asp Gly
115 120 125
Arg Pro Gly Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Gln
130 135 140
Gly Asn Thr Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Leu Ile Asn
145 150 155 160
Cys Asn Leu Lys Ser Leu Ala Asp Val Ser Glu Arg Leu Ala Val Gln
165 170 175
Asp Arg Thr Glu Pro Ile Asp Gln Val
180 185
<210> 56
<211> 186
<212> PRT
<213> 毛果杨(Populus trichocarpa) x 美国黑杨(Populus deltoides)
<220>
<223> 加利福尼亚白杨(西方胶杨,黑杨)
x 东部杨木(Eastern cottonwood), 品种H11-11, 未知蛋白,
克隆 WS0133_I04, GenBank登录号ABK96505.1
<400> 56
Met Asn Gly Ser Asp Ala Tyr Ser Ala Thr Glu Ala Gln Tyr Val Arg
1 5 10 15
Arg His His Lys His Glu Pro Arg Glu Asn Gln Cys Thr Ser Ala Leu
20 25 30
Val Lys His Ile Lys Ala Pro Ala His Leu Val Trp Ser Leu Val Arg
35 40 45
Arg Phe Asp Gln Pro Gln Arg Tyr Lys Pro Phe Val Ser Arg Cys Val
50 55 60
Met Asn Gly Glu Leu Gly Ile Gly Ser Val Arg Glu Val Asn Val Lys
65 70 75 80
Ser Gly Leu Pro Ala Thr Thr Ser Thr Glu Arg Leu Glu Leu Leu Asp
85 90 95
Asp Glu Glu His Ile Leu Gly Val Gln Ile Val Gly Gly Asp His Arg
100 105 110
Leu Lys Asn Tyr Ser Ser Ile Met Thr Val His Pro Glu Phe Ile Asp
115 120 125
Gly Arg Pro Gly Thr Leu Val Ile Glu Ser Phe Ile Val Asp Val Pro
130 135 140
Asp Gly Asn Thr Lys Asp Glu Thr Cys Tyr Phe Val Lys Ala Leu Ile
145 150 155 160
Arg Cys Asn Leu Lys Ser Leu Ala Asp Val Ser Glu Arg Met Ala Val
165 170 175
Gln Asp Arg Val Glu Pro Val Asn Gln Phe
180 185
<210> 57
<211> 188
<212> PRT
<213> 豌豆(Pisum sativum)
<220>
<223> 豌豆AT-富含型元件结合因子3 (PsATF, ATF3),
可能的转录因子, GenBank登录号
AAV85853.1
<400> 57
Met Asn Asn Gly Gly Glu Gln Tyr Ser Ala Ile Glu Thr Gln Tyr Ile
1 5 10 15
Arg Arg Arg His Lys His Asp Leu Arg Asp Asn Gln Cys Ser Ser Ala
20 25 30
Leu Val Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val
35 40 45
Arg Arg Phe Asp Gln Pro Gln Lys Tyr Lys Pro Phe Val Ser Arg Cys
50 55 60
Ile Met Gln Gly Asp Leu Gly Ile Gly Ser Val Arg Glu Val Asn Val
65 70 75 80
Lys Ser Gly Leu Pro Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu
85 90 95
Asp Asp Glu Glu His Ile Leu Gly Ile Arg Ile Val Gly Gly Asp His
100 105 110
Arg Leu Arg Asn Tyr Ser Ser Val Ile Thr Val His Pro Glu Val Ile
115 120 125
Asp Gly Arg Pro Gly Thr Met Val Ile Glu Ser Phe Val Val Asp Val
130 135 140
Pro Glu Gly Asn Thr Arg Asp Glu Thr Cys Tyr Phe Val Glu Ala Leu
145 150 155 160
Ile Arg Gly Asn Leu Ser Ser Leu Ala Asp Val Ser Glu Arg Met Ala
165 170 175
Val Gln Gly Arg Thr Asp Pro Ile Asn Val Asn Pro
180 185
<210> 58
<211> 177
<212> PRT
<213> 葡萄(Vitis vinifera)
<220>
<223> 酿酒葡萄PN40024未命名蛋白质产物, 基因座
标签GSVIVT00027009001, GenBank登录号CAO39744.1
<400> 58
Met Glu Ala Gln Val Ile Cys Arg His His Ala His Glu Pro Arg Glu
1 5 10 15
Asn Gln Cys Ser Ser Val Leu Val Arg His Val Lys Ala Pro Ala Asn
20 25 30
Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Lys Tyr Lys
35 40 45
Pro Phe Val Ser Arg Cys Val Val Gln Gly Asp Leu Arg Ile Gly Ser
50 55 60
Val Arg Glu Val Asn Val Lys Thr Gly Leu Pro Ala Thr Thr Ser Thr
65 70 75 80
Glu Arg Leu Glu Leu Phe Asp Asp Asp Glu His Val Leu Gly Ile Lys
85 90 95
Ile Leu Asp Gly Asp His Arg Leu Arg Asn Tyr Ser Ser Val Ile Thr
100 105 110
Val His Pro Glu Ile Ile Asp Gly Arg Pro Gly Thr Leu Val Ile Glu
115 120 125
Ser Phe Val Val Asp Val Pro Glu Gly Asn Thr Lys Asp Asp Thr Cys
130 135 140
Tyr Phe Val Arg Ala Leu Ile Asn Cys Asn Leu Lys Cys Leu Ala Glu
145 150 155 160
Val Ser Glu Arg Met Ala Met Leu Gly Arg Val Glu Pro Ala Asn Ala
165 170 175
Val
<210> 59
<211> 178
<212> PRT
<213> 葡萄(Vitis vinifera)
<220>
<223> 酿酒葡萄黑皮诺假设蛋白, 克隆
ENTAV 115, 基因座标签VITISV_004915, GenBank登录号
CAN82501.1
<400> 59
Met Met Glu Ala Gln Val Ile Cys Arg His His Ala His Glu Pro Arg
1 5 10 15
Glu Asn Gln Cys Ser Ser Val Leu Val Arg His Val Lys Ala Pro Ala
20 25 30
Asn Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Lys Tyr
35 40 45
Lys Pro Phe Val Ser Arg Cys Val Val Gln Gly Asp Leu Arg Ile Gly
50 55 60
Ser Val Arg Glu Val Asn Val Lys Thr Gly Leu Pro Ala Thr Thr Ser
65 70 75 80
Thr Glu Arg Leu Glu Leu Phe Asp Asp Asp Glu His Val Leu Gly Ile
85 90 95
Lys Ile Leu Asp Gly Asp His Arg Leu Arg Asn Tyr Ser Ser Val Ile
100 105 110
Thr Val His Pro Glu Ile Ile Asp Gly Arg Pro Gly Thr Leu Val Ile
115 120 125
Glu Ser Phe Val Val Asp Val Pro Glu Gly Asn Thr Lys Asp Asp Thr
130 135 140
Cys Tyr Phe Val Arg Ala Leu Ile Asn Cys Asn Leu Lys Cys Leu Ala
145 150 155 160
Glu Val Ser Glu Arg Met Ala Met Leu Gly Arg Val Glu Pro Ala Asn
165 170 175
Ala Val
<210> 60
<211> 193
<212> PRT
<213> 花生(Arachis hypogaea)
<220>
<223> 花生发病诱导蛋白(PIP), GenBank
登录号ACG76109.1
<220>
<221> 变体
<222> (162)...(162)
<223> Xaa = 任何氨基酸
<400> 60
Met Met Asn Gly Ser Cys Gly Gly Gly Gly Gly Gly Glu Ala Tyr Gly
1 5 10 15
Ala Ile Glu Ala Gln Tyr Ile Arg Arg His His Arg His Glu Pro Arg
20 25 30
Asp Asn Gln Cys Thr Ser Ala Leu Val Lys His Ile Arg Ala Pro Val
35 40 45
His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Lys Tyr
50 55 60
Lys Pro Phe Val Ser Arg Cys Ile Met Gln Gly Asp Leu Gly Ile Gly
65 70 75 80
Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala Thr Thr Ser
85 90 95
Thr Glu Arg Leu Glu Gln Leu Asp Asp Glu Glu His Ile Leu Gly Ile
100 105 110
Arg Ile Val Gly Gly Asp His Arg Leu Arg Asn Tyr Ser Ser Ile Ile
115 120 125
Thr Val His Pro Glu Val Ile Glu Gly Arg Pro Gly Thr Met Val Ile
130 135 140
Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr Lys Asp Glu Thr
145 150 155 160
Cys Xaa Phe Val Glu Ala Leu Ile Arg Cys Asn Leu Ser Ser Leu Ala
165 170 175
Asp Val Ser Glu Arg Met Ala Val Gln Gly Arg Thr Asp Pro Ile Asn
180 185 190
Gln
<210> 61
<211> 217
<212> PRT
<213> 玉米(Zea mays)
<220>
<223> 玉米AT-富含型元件结合因子3, 克隆 300908,
GenBank登录号ACG39386.1
<400> 61
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Gly Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Ala Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Phe Val Gly Gly Asp His Arg
130 135 140
Leu Gln Asn Tyr Ser Ser Ile Leu Thr Val His Pro Glu Val Ile Asp
145 150 155 160
Gly Arg Pro Gly Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro
165 170 175
Asp Gly Asn Thr Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Leu Leu
180 185 190
Lys Cys Asn Leu Arg Ser Leu Ala Glu Val Ser Glu Gly Gln Val Ile
195 200 205
Met Asp Gln Thr Glu Pro Leu Asp Arg
210 215
<210> 62
<211> 217
<212> PRT
<213> 玉米(Zea mays)
<220>
<223> 玉米株B73, 未知蛋白, 克隆 ZM_BFb0036A01,
GenBank登录号ACF80077.1
<400> 62
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Ala Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Pro Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Phe Val Gly Gly Asp His Arg
130 135 140
Leu Gln Asn Tyr Ser Ser Ile Leu Thr Val His Pro Glu Val Ile Asp
145 150 155 160
Gly Arg Pro Gly Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro
165 170 175
Asp Gly Asn Thr Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Leu Leu
180 185 190
Lys Cys Asn Leu Arg Ser Leu Ala Glu Val Ser Glu Gly Gln Val Ile
195 200 205
Met Asp Gln Thr Glu Pro Leu Asp Arg
210 215
<210> 63
<211> 206
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻粳组, 日本晴品种, 假设
蛋白Os06g0528300, GenBank登录号NP_001057772.1
<400> 63
Met Asn Gly Val Gly Gly Ala Gly Gly Ala Ala Ala Gly Lys Leu Pro
1 5 10 15
Met Val Ser His Arg Arg Val Gln Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Glu Leu Arg Glu Glu Glu Met Glu Tyr Ile Arg Arg Phe His Arg His
35 40 45
Glu Pro Ser Ser Asn Gln Cys Thr Ser Phe Ala Ala Lys His Ile Lys
50 55 60
Ala Pro Leu His Thr Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Phe Val Arg Asn Cys Val Met Arg Glu Asn Ile
85 90 95
Ile Ala Thr Gly Cys Ile Arg Glu Val Asn Val Gln Ser Gly Leu Pro
100 105 110
Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His
115 120 125
Ile Leu Lys Val Asn Phe Ile Gly Gly Asp His Met Leu Lys Asn Tyr
130 135 140
Ser Ser Ile Leu Thr Val His Ser Glu Val Ile Asp Gly Gln Leu Gly
145 150 155 160
Thr Leu Val Val Glu Ser Phe Ile Val Asp Val Pro Glu Gly Asn Thr
165 170 175
Lys Asp Asp Ile Ser Tyr Phe Ile Glu Asn Val Leu Arg Cys Asn Leu
180 185 190
Arg Thr Leu Ala Asp Val Ser Glu Glu Arg Leu Ala Asn Pro
195 200 205
<210> 64
<211> 206
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻籼组, 品种93-11, 假设蛋白
OsI_23215, GenBank登录号EAZ01188.1
<400> 64
Met Asn Gly Ala Gly Gly Ala Gly Gly Ala Ala Ala Gly Lys Leu Pro
1 5 10 15
Met Val Ser His Arg Gln Val Gln Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Glu Leu Arg Glu Glu Glu Met Glu Tyr Ile Arg Gln Phe His Arg His
35 40 45
Glu Pro Ser Ser Asn Gln Cys Thr Ser Phe Val Ala Lys His Ile Lys
50 55 60
Ala Pro Leu Gln Thr Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Phe Val Arg Lys Cys Val Met Arg Glu Asn Ile
85 90 95
Ile Ala Thr Gly Cys Val Arg Glu Val Asn Val Gln Ser Gly Leu Pro
100 105 110
Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His
115 120 125
Ile Leu Lys Val Lys Phe Ile Gly Gly Asp His Met Leu Lys Asn Tyr
130 135 140
Ser Ser Ile Leu Thr Ile His Ser Glu Val Ile Asp Gly Gln Leu Gly
145 150 155 160
Thr Leu Val Val Glu Ser Phe Val Val Asp Ile Pro Glu Gly Asn Thr
165 170 175
Lys Asp Asp Ile Cys Tyr Phe Ile Glu Asn Ile Leu Arg Cys Asn Leu
180 185 190
Met Thr Leu Ala Asp Val Ser Glu Glu Arg Leu Ala Asn Pro
195 200 205
<210> 65
<211> 205
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻粳组, 日本晴品种, 假设
蛋白OsJ_06125, GenBank登录号EAZ22456.1
<400> 65
Met Val Glu Val Gly Gly Gly Ala Ala Glu Ala Ala Ala Gly Arg Arg
1 5 10 15
Trp Arg Leu Ala Asp Glu Arg Cys Asp Leu Arg Ala Ala Glu Thr Glu
20 25 30
Tyr Val Arg Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser
35 40 45
Ser Ala Val Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser
50 55 60
Leu Val Arg Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser
65 70 75 80
Arg Cys Glu Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val
85 90 95
Asn Val Lys Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu
100 105 110
Leu Leu Asp Asp Asn Glu His Ile Leu Ser Val Arg Phe Val Gly Gly
115 120 125
Asp His Arg Leu Lys Asn Tyr Ser Ser Ile Leu Thr Val His Pro Glu
130 135 140
Val Ile Asp Gly Arg Pro Gly Thr Leu Val Ile Glu Ser Phe Val Val
145 150 155 160
Asp Val Pro Glu Gly Asn Thr Lys Asp Glu Thr Cys Tyr Phe Val Glu
165 170 175
Ala Leu Leu Lys Cys Asn Leu Lys Ser Leu Ala Glu Val Ser Glu Arg
180 185 190
Leu Val Cys Gln Gly Pro Asn Arg Ala Pro Ser Thr Arg
195 200 205
<210> 66
<211> 204
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻粳组, 日本晴品种, 假设
蛋白Os02g0255500, 类似于延伸(片段),
GenBank登录号NP_001046464.1
<400> 66
Met Val Glu Val Gly Gly Gly Ala Ala Glu Ala Ala Ala Gly Arg Arg
1 5 10 15
Trp Arg Leu Ala Asp Glu Arg Cys Asp Leu Arg Ala Ala Glu Thr Glu
20 25 30
Tyr Val Arg Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser
35 40 45
Ser Ala Val Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser
50 55 60
Leu Val Arg Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser
65 70 75 80
Arg Cys Glu Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val
85 90 95
Asn Val Lys Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu
100 105 110
Leu Leu Asp Asp Asn Glu His Ile Leu Ser Val Arg Phe Val Gly Gly
115 120 125
Asp His Arg Leu Lys Asn Tyr Ser Ser Ile Leu Thr Val His Pro Glu
130 135 140
Val Ile Asp Gly Arg Pro Gly Thr Leu Val Ile Glu Ser Phe Val Val
145 150 155 160
Asp Val Pro Glu Gly Asn Thr Lys Asp Glu Thr Cys Tyr Phe Val Glu
165 170 175
Ala Leu Leu Lys Cys Asn Leu Lys Ser Leu Ala Glu Val Ser Glu Arg
180 185 190
Leu Val Val Lys Asp Gln Thr Glu Pro Leu Asp Arg
195 200
<210> 67
<211> 199
<212> PRT
<213> 蒺藜状苜蓿(Medicago truncatula)
<220>
<223> 蒺藜状苜蓿未知蛋白, 克隆 MTYFP_FQ_FR_FS1G-G-11,
GenBank登录号ACJ86004.1
<400> 67
Met Glu Lys Met Asn Gly Thr Glu Asn Asn Gly Val Phe Asn Ser Thr
1 5 10 15
Glu Met Glu Tyr Ile Arg Arg His His Asn Gln Gln Pro Gly Glu Asn
20 25 30
Gln Cys Ser Ser Ala Leu Val Lys His Ile Arg Ala Pro Val Pro Leu
35 40 45
Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Lys Tyr Lys Pro
50 55 60
Phe Val Ser Arg Cys Val Val Arg Gly Asn Leu Glu Ile Gly Ser Leu
65 70 75 80
Arg Glu Val Asp Val Lys Ser Gly Leu Pro Ala Thr Thr Ser Thr Glu
85 90 95
Arg Leu Glu Val Leu Asp Asp Asn Glu His Ile Leu Ser Ile Arg Ile
100 105 110
Ile Gly Gly Asp His Arg Leu Arg Asn Tyr Ser Ser Ile Met Ser Leu
115 120 125
His Pro Glu Ile Ile Asp Gly Arg Pro Gly Thr Leu Val Ile Glu Ser
130 135 140
Phe Val Val Asp Val Pro Glu Gly Asn Thr Lys Asp Glu Thr Cys Tyr
145 150 155 160
Phe Val Glu Ala Leu Ile Lys Cys Asn Leu Lys Ser Leu Ser Asp Val
165 170 175
Ser Glu Gly His Ala Val Gln Asp Leu Thr Glu Pro Leu Asp Arg Val
180 185 190
His Glu Leu Leu Ile Ser Gly
195
<210> 68
<211> 199
<212> PRT
<213> 蒺藜状苜蓿(Medicago truncatula)
<220>
<223> 蒺藜状苜蓿未知蛋白, 克隆 MTYF1_F2_F3_FY1G-K-4,
GenBank登录号ACJ83958.1
<400> 68
Met Glu Lys Met Asn Gly Thr Glu Asn Asn Gly Val Phe Asn Ser Thr
1 5 10 15
Glu Met Glu Tyr Ile Arg Arg His His Asn Gln Gln Pro Gly Glu Asn
20 25 30
Gln Cys Ser Ser Ala Leu Val Lys His Ile Arg Ala Pro Val Pro Leu
35 40 45
Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Lys Tyr Lys Pro
50 55 60
Phe Val Ser Arg Cys Val Val Arg Gly Asn Leu Glu Ile Gly Ser Leu
65 70 75 80
Arg Glu Val Asp Val Lys Ser Gly Leu Pro Ala Thr Thr Ser Thr Glu
85 90 95
Arg Leu Glu Val Leu Asp Asp Asn Glu His Ile Leu Ser Ile Arg Ile
100 105 110
Ile Gly Gly Asp His Arg Leu Arg Asn Tyr Ser Ser Ile Met Ser Leu
115 120 125
His Pro Glu Ile Ile Asp Gly Arg Pro Gly Thr Leu Val Ile Glu Ser
130 135 140
Phe Val Val Asp Val Pro Glu Gly Asn Thr Lys Asp Glu Thr Cys Tyr
145 150 155 160
Phe Val Glu Ala Leu Ile Lys Cys Asn Leu Lys Ser Leu Ser Asp Val
165 170 175
Ser Glu Gly His Ala Ala Gln Asp Leu Thr Glu Pro Leu Asp Arg Met
180 185 190
His Glu Leu Leu Ile Ser Gly
195
<210> 69
<211> 197
<212> PRT
<213> 玉米(Zea mays)
<220>
<223> 玉米CAPIP1蛋白, 克隆 244179, GenBank登录号
ACG34726.1
<400> 69
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His
20 25 30
Ala Pro Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Gln Leu Val Arg Arg Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Asn Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Leu Ile Asp Gln
195
<210> 70
<211> 197
<212> PRT
<213> 玉米(Zea mays)
<220>
<223> 玉米CAPIP1蛋白, 克隆 1448906, GenBank登录号
ACG26022.1
<400> 70
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His
20 25 30
Ala Pro Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Arg Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Asn Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Leu Ile Asp Gln
195
<210> 71
<211> 212
<212> PRT
<213> 玉米(Zea mays)
<220>
<223> 玉米株B73 未知蛋白, 克隆 ZM_BFc0183D21,
GenBank登录号ACF86162.1
<400> 71
Met Val Met Val Glu Met Asp Gly Gly Val Gly Gly Gly Gly Gly Gly
1 5 10 15
Gly Gln Thr Pro Ala Pro Arg Arg Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His
35 40 45
Glu Pro Arg Glu His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys
50 55 60
Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu Met Lys Gly Asn Ile
85 90 95
Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala
100 105 110
Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile
115 120 125
Leu Ser Val Arg Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser
130 135 140
Ser Ile Leu Thr Val His Pro Glu Val Ile Asp Gly Arg Pro Gly Thr
145 150 155 160
Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr Lys
165 170 175
Asp Glu Thr Cys Tyr Phe Val Glu Ala Leu Leu Lys Cys Asn Leu Lys
180 185 190
Ser Leu Ala Glu Val Ser Glu Arg Gln Val Val Lys Asp Gln Thr Glu
195 200 205
Pro Leu Asp Arg
210
<210> 72
<211> 205
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻粳组, 日本晴品种, 保守
假设蛋白 Os06g0527800, GenBank登录号
NP_001057771.1
<400> 72
Met Asn Gly Ala Gly Gly Ala Gly Gly Ala Ala Ala Gly Lys Leu Pro
1 5 10 15
Met Val Ser His Arg Arg Val Gln Cys Arg Leu Ala Asp Lys Arg Cys
20 25 30
Glu Leu Arg Glu Glu Glu Met Glu Tyr Ile Arg Gln Phe His Arg His
35 40 45
Glu Pro Ser Ser Asn Gln Cys Thr Ser Phe Val Ala Lys His Ile Lys
50 55 60
Ala Pro Leu Gln Thr Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Phe Val Arg Lys Cys Val Met Arg Glu Asn Ile
85 90 95
Ile Val Thr Gly Cys Val Arg Glu Val Asn Val Gln Ser Gly Leu Pro
100 105 110
Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His
115 120 125
Ile Leu Lys Val Lys Phe Ile Gly Gly Asp His Met Leu Lys Asn Tyr
130 135 140
Ser Ser Ile Leu Thr Ile His Ser Glu Val Ile Asp Gly Gln Leu Gly
145 150 155 160
Thr Leu Val Val Glu Ser Phe Val Val Asp Ile Pro Asp Gly Asn Thr
165 170 175
Lys Asp Asp Ile Cys Tyr Phe Ile Glu Asn Val Leu Arg Cys Asn Leu
180 185 190
Met Thr Leu Ala Asp Val Ser Glu Glu Arg Leu Ala Asn
195 200 205
<210> 73
<211> 197
<212> PRT
<213> 玉米(Zea mays)
<220>
<223> 玉米株B73 未知蛋白, 克隆 ZM_BFc0063E17,
GenBank登录号ACF85073.1
<400> 73
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Thr Glu Tyr Val Arg Arg Leu His Arg His
20 25 30
Ala Pro Ala Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Ser Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Lys Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Pro Ile Asp Gln
195
<210> 74
<211> 206
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻籼组, 品种93-11, 假设蛋白
OsI_23218, GenBank登录号EAZ01191.1
<400> 74
Met Asn Gly Val Gly Gly Ala Gly Gly Ala Ala Ala Gly Lys Leu Pro
1 5 10 15
Met Val Ser His Arg Arg Val Gln Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Glu Leu Arg Glu Glu Glu Met Glu Tyr Ile Arg Arg Phe His Arg His
35 40 45
Glu Pro Ser Ser Asn Gln Cys Thr Ser Phe Ala Ala Lys His Ile Lys
50 55 60
Ala Pro Leu His Thr Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Phe Val Arg Asn Cys Val Met Arg Glu Asn Ile
85 90 95
Ile Ala Thr Gly Cys Ile Arg Glu Val Asn Val Gln Ser Gly Leu Pro
100 105 110
Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His
115 120 125
Ile Leu Lys Val Lys Phe Ile Gly Gly Asp His Met Leu Lys Asn Tyr
130 135 140
Ser Ser Ile Leu Thr Val His Ser Glu Val Ile Asp Gly Gln Leu Gly
145 150 155 160
Thr Leu Val Val Glu Ser Phe Ile Val Asp Val Leu Glu Gly Asn Thr
165 170 175
Lys Asp Asp Ile Ser Tyr Phe Ile Glu Asn Val Leu Arg Cys Asn Leu
180 185 190
Arg Thr Leu Ala Asp Val Ser Glu Glu Arg Leu Ala Asn Pro
195 200 205
<210> 75
<211> 209
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻粳组, 日本晴品种, 保守
假设蛋白 Os05g0213500, GenBank登录号
NP_001054923.1
<400> 75
Met Val Gly Leu Val Gly Gly Gly Gly Trp Arg Val Gly Asp Asp Ala
1 5 10 15
Ala Gly Gly Gly Gly Gly Gly Ala Val Ala Ala Gly Ala Ala Ala Ala
20 25 30
Ala Glu Ala Glu His Met Arg Arg Leu His Ser His Ala Pro Gly Glu
35 40 45
His Gln Cys Ser Ser Ala Leu Val Lys His Ile Lys Ala Pro Val His
50 55 60
Leu Val Trp Ser Leu Val Arg Ser Phe Asp Gln Pro Gln Arg Tyr Lys
65 70 75 80
Pro Phe Val Ser Arg Cys Val Val Arg Gly Gly Asp Leu Glu Ile Gly
85 90 95
Ser Val Arg Glu Val Asn Val Lys Thr Gly Leu Pro Ala Thr Thr Ser
100 105 110
Thr Glu Arg Leu Glu Leu Leu Asp Asp Asp Glu His Ile Leu Ser Val
115 120 125
Lys Phe Val Gly Gly Asp His Arg Leu Arg Asn Tyr Ser Ser Ile Val
130 135 140
Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly Thr Leu Val Ile
145 150 155 160
Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr Lys Asp Glu Thr
165 170 175
Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu Thr Ser Leu Ala
180 185 190
Glu Val Ser Glu Arg Leu Ala Val Gln Ser Pro Thr Ser Pro Leu Glu
195 200 205
Gln
<210> 76
<211> 180
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻粳组, 日本晴品种, Bet v I
过敏原样蛋白, 克隆 OSJNBa0052K15,基因
OSJNBa0052K15.17, GenBank登录号BAD29692.1
<400> 76
Met Val Glu Met Asp Ala Gly Gly Arg Pro Glu Pro Ser Pro Pro Ser
1 5 10 15
Gly Gln Cys Ser Ser Ala Val Thr Met Arg Ile Asn Ala Pro Val His
20 25 30
Leu Val Trp Ser Ile Val Arg Arg Phe Glu Glu Pro His Ile Phe Gln
35 40 45
Pro Phe Val Arg Gly Cys Thr Met Arg Gly Ser Thr Ser Leu Ala Val
50 55 60
Gly Cys Val Arg Glu Val Asp Phe Lys Ser Gly Phe Pro Ala Lys Ser
65 70 75 80
Ser Val Glu Arg Leu Glu Ile Leu Asp Asp Lys Glu His Val Phe Gly
85 90 95
Val Arg Ile Ile Gly Gly Asp His Arg Leu Lys Asn Tyr Ser Ser Val
100 105 110
Leu Thr Ala Lys Pro Glu Val Ile Asp Gly Glu Pro Ala Thr Leu Val
115 120 125
Ser Glu Ser Phe Val Val Asp Val Pro Glu Gly Asn Thr Ala Asp Glu
130 135 140
Thr Arg His Phe Val Glu Phe Leu Ile Arg Cys Asn Leu Arg Ser Leu
145 150 155 160
Ala Met Val Ser Gln Arg Leu Leu Leu Ala Gln Gly Asp Leu Ala Glu
165 170 175
Pro Pro Ala Gln
180
<210> 77
<211> 176
<212> PRT
<213> 葡萄(Vitis vinifera)
<220>
<223> 酿酒葡萄黑皮诺假设蛋白, 克隆
ENTAV 115, 基因座标签VITISV_029498, GenBank登录号
CAN64668.1
<400> 77
Met Asn Gly Asn Gly Leu Ser Ser Met Glu Ser Glu Tyr Ile Arg Arg
1 5 10 15
His His Arg His Glu Pro Ala Glu Asn Gln Cys Ser Ser Ala Leu Val
20 25 30
Lys His Ile Lys Ala Pro Val Pro Leu Val Trp Ser Leu Val Arg Arg
35 40 45
Phe Asp Gln Pro Gln Lys Tyr Lys Pro Phe Ile Ser Arg Cys Val Val
50 55 60
Gln Gly Asn Leu Glu Ile Gly Ser Leu Arg Glu Val Asp Val Lys Ser
65 70 75 80
Gly Leu Pro Ala Thr Thr Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp
85 90 95
Asp Glu His Ile Leu Ser Met Arg Ile Ile Gly Gly Asp His Arg Leu
100 105 110
Arg Asn Tyr Ser Ser Ile Ile Ser Leu His Pro Glu Ile Ile Asp Gly
115 120 125
Arg Pro Gly Thr Met Val Ile Glu Ser Tyr Val Val Asp Val Pro Glu
130 135 140
Gly Asn Thr Lys Asp Glu Thr Cys Tyr Phe Ser Leu Ala Asp Val Ser
145 150 155 160
Glu Arg Leu Ala Val Ala Gly Thr Val Thr Glu Pro Ile Asp Arg Met
165 170 175
<210> 78
<211> 180
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻籼组, 品种93-11, 假设蛋白,
基因座标签OsI_06615, GenBank登录号EEC72859.1
<400> 78
Met Val Glu Met Asp Ala Gly Gly Arg Pro Glu Pro Ser Pro Pro Ser
1 5 10 15
Gly Gln Cys Ser Ser Ala Val Thr Met Arg Ile Asn Ala Pro Val His
20 25 30
Leu Val Trp Ser Ile Val Arg Arg Phe Glu Glu Pro His Ile Phe Gln
35 40 45
Pro Phe Val Arg Gly Cys Thr Met Arg Gly Ser Thr Ser Leu Ala Val
50 55 60
Gly Cys Val Arg Glu Val Asp Phe Lys Ser Gly Phe Ser Ala Lys Ser
65 70 75 80
Ser Val Glu Arg Leu Glu Ile Leu Asp Asp Lys Glu His Val Phe Gly
85 90 95
Val Arg Ile Ile Gly Gly Asp His Arg Leu Lys Asn Tyr Ser Ser Val
100 105 110
Leu Thr Ala Lys Pro Glu Val Ile Asp Gly Glu Pro Ala Thr Leu Val
115 120 125
Ser Glu Ser Phe Val Ile Asp Val Pro Glu Gly Asn Thr Ala Asp Glu
130 135 140
Thr Arg His Phe Val Glu Phe Leu Ile Arg Cys Asn Leu Arg Ser Leu
145 150 155 160
Ala Met Val Ser Gln Arg Leu Leu Leu Ala Gln Gly Asp Leu Ala Glu
165 170 175
Pro Pro Ala Gln
180
<210> 79
<211> 215
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻粳组, 日本晴品种, 假设
蛋白, 基因座标签OsJ_10498, GenBank登录号
EAZ26598.1
<400> 79
Met Pro Cys Ile Pro Ala Ser Ser Pro Gly Ile Pro His Gln His Gln
1 5 10 15
His Gln His His Arg Ala Leu Ala Gly Val Gly Met Ala Val Gly Cys
20 25 30
Ala Ala Glu Ala Ala Val Ala Ala Ala Gly Val Ala Gly Thr Arg Cys
35 40 45
Gly Ala His Asp Gly Glu Val Pro Met Glu Val Ala Arg His His Glu
50 55 60
His Ala Glu Pro Gly Ser Gly Arg Cys Cys Ser Ala Val Val Gln His
65 70 75 80
Val Ala Ala Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp
85 90 95
Gln Pro Gln Ala Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala
100 105 110
Gly Asp Gly Gly Leu Gly Lys Val Arg Glu Arg Leu Glu Ile Leu Asp
115 120 125
Asp Glu Ser His Val Leu Ser Phe Arg Val Val Gly Gly Glu His Arg
130 135 140
Leu Lys Asn Tyr Leu Ser Val Thr Thr Val His Pro Ser Pro Ser Ala
145 150 155 160
Pro Thr Ala Ala Thr Val Val Val Glu Ser Tyr Val Val Asp Val Pro
165 170 175
Pro Gly Asn Thr Pro Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val
180 185 190
Lys Cys Asn Leu Gln Ser Leu Ala Lys Thr Ala Glu Lys Leu Ala Ala
195 200 205
Gly Ala Arg Ala Ala Gly Ser
210 215
<210> 80
<211> 186
<212> PRT
<213> 藏边大黄(Rheum australe)
<220>
<223> 喜马大黄病原诱导蛋白样蛋白,
GenBank登录号ACH63237.1
<400> 80
Met Asn Gly Asp Gly Tyr Gly Gly Ser Glu Glu Glu Phe Val Lys Arg
1 5 10 15
Tyr His Glu His Val Leu Ala Asp His Gln Cys Ser Ser Val Leu Val
20 25 30
Glu His Ile Asn Ala Pro Leu His Leu Val Trp Ser Leu Val Arg Ser
35 40 45
Phe Asp Gln Pro Gln Lys Tyr Lys Pro Phe Val Ser Arg Cys Val Val
50 55 60
Gln Gly Gly Asp Leu Glu Ile Gly Ser Val Arg Glu Val Asp Val Lys
65 70 75 80
Ser Gly Leu Pro Ala Thr Thr Ser Met Glu Glu Leu Glu Leu Leu Asp
85 90 95
Asp Lys Glu His Val Leu Arg Val Lys Phe Val Gly Gly Asp His Arg
100 105 110
Leu Lys Asn Tyr Ser Ser Ile Val Ser Leu His Pro Glu Ile Ile Gly
115 120 125
Gly Arg Ser Gly Thr Met Val Ile Glu Ser Phe Ile Val Asp Ile Ala
130 135 140
Asp Gly Asn Thr Lys Glu Glu Thr Cys Tyr Phe Ile Glu Ser Leu Ile
145 150 155 160
Asn Cys Asn Leu Lys Ser Leu Ser Cys Val Ser Glu Arg Leu Ala Val
165 170 175
Glu Asp Ile Ala Glu Arg Ile Ala Gln Met
180 185
<210> 81
<211> 254
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻粳组, 日本晴品种, 假设
蛋白, 基因座标签OsJ_016770, GenBank登录号
EAZ33287.1
<400> 81
Met Val Gly Leu Val Gly Gly Gly Gly Trp Arg Val Gly Asp Asp Ala
1 5 10 15
Ala Gly Gly Gly Gly Gly Gly Ala Val Ala Ala Gly Ala Ala Ala Ala
20 25 30
Ala Glu Ala Glu His Met Arg Arg Leu His Ser Gln Gly Pro Arg Arg
35 40 45
Ala Pro Val Gln Leu Arg Ala Arg Gln Ala His Gln Gly Ser Cys Ser
50 55 60
Pro Pro Arg Ile Glu Cys Ala Asn Phe Ala Val Phe Leu Ala Ala Arg
65 70 75 80
Asp Pro Lys Ile Val Trp Ser Leu Val Arg Ser Phe Asp Gln Pro Gln
85 90 95
Arg Tyr Lys Pro Phe Val Ser Arg Cys Val Val Arg Gly Gly Asp Leu
100 105 110
Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys Thr Gly Leu Pro Ala
115 120 125
Thr Thr Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asp Glu His Ile
130 135 140
Leu Ser Val Lys Phe Val Gly Gly Asp His Arg Leu Arg Asn Tyr Ser
145 150 155 160
Ser Ile Val Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly Thr
165 170 175
Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr Lys
180 185 190
Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu Thr
195 200 205
Ser Leu Ala Glu Met Val Arg Met Ile Ser Leu Val Leu Pro Phe Met
210 215 220
Leu Val Asp Arg Met Ser Gly Ile Thr Cys Glu Ser His Leu Glu Thr
225 230 235 240
Thr Leu Val Arg Cys Gly Glu Tyr Ala Val Leu Ala His Val
245 250
<210> 82
<211> 186
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻粳组, 日本晴品种, 假设
蛋白, 基因座标签OsJ_005784, GenBank登录号
EAZ22301.1
<400> 82
Met Glu Pro His Met Glu Arg Ala Leu Arg Glu Ala Val Ala Ser Glu
1 5 10 15
Ala Glu Arg Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Gly
20 25 30
Trp Asn Ala Pro Leu Ala Ala Val Trp Pro His Arg Ala Arg Val Arg
35 40 45
Pro Thr Arg Ser Gly Thr Ser Thr Ser Ser Ser Arg Ala Ser Ser Pro
50 55 60
Pro Gly Asp Gly Ala Thr Val Gly Ser Val Arg Glu Val Ala Val Val
65 70 75 80
Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp
85 90 95
Asp Asp Arg His Val Leu Ser Phe Arg Val Val Gly Gly Asp His Arg
100 105 110
Leu Arg Asn Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Ser Ser Pro
115 120 125
Ser Ser Pro Pro Arg Pro Tyr Cys Val Val Val Glu Ser Tyr Val Val
130 135 140
Asp Val Pro Glu Gly Asn Thr Glu Glu Asp Thr Arg Met Phe Thr Asp
145 150 155 160
Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Ala Val Ala Thr Ser
165 170 175
Ser Ser Pro Pro Ala Ala Gly Asn His His
180 185
<210> 83
<211> 150
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻粳组, 日本晴品种, 假设
蛋白, 基因座标签OsJ_005938, GenBank登录号
EAZ22455.1
<400> 83
Met Glu Val Val Trp Ser Ile Val Arg Arg Phe Glu Glu Pro His Ile
1 5 10 15
Phe Gln Pro Phe Val Arg Gly Cys Thr Met Arg Gly Ser Thr Ser Leu
20 25 30
Ala Val Gly Cys Val Arg Glu Val Asp Phe Lys Ser Gly Phe Pro Ala
35 40 45
Lys Ser Ser Val Glu Arg Leu Glu Ile Leu Asp Asp Lys Glu His Val
50 55 60
Phe Gly Val Arg Ile Ile Gly Gly Asp His Arg Leu Lys Asn Tyr Ser
65 70 75 80
Ser Val Leu Thr Ala Lys Pro Glu Val Ile Asp Gly Glu Pro Ala Thr
85 90 95
Leu Val Ser Glu Ser Phe Val Val Asp Val Pro Glu Gly Asn Thr Ala
100 105 110
Asp Glu Thr Arg His Phe Val Glu Phe Leu Ile Arg Cys Asn Leu Arg
115 120 125
Ser Leu Ala Met Val Ser Gln Arg Leu Leu Leu Ala Gln Gly Asp Leu
130 135 140
Ala Glu Pro Pro Gly Gln
145 150
<210> 84
<211> 206
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻粳组, 日本晴品种, 假设
蛋白, 基因座标签OsJ_018129, GenBank登录号
EAZ34646.1
<400> 84
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Pro Gln His Ser Arg Ile
1 5 10 15
Gly Gly Cys Gly Gly Gly Gly Val Leu Lys Ala Ala Gly Ala Ala Gly
20 25 30
His Ala Ala Ser Cys Val Ala Val Pro Ala Glu Val Ala Arg His His
35 40 45
Glu His Ala Ala Gly Val Gly Gln Cys Cys Ser Ala Val Val Gln Ala
50 55 60
Ile Ala Ala Pro Val Asp Ala Val Trp Arg Thr Ser Thr Ser Ser Gly
65 70 75 80
Ala Ala Ala Ser Trp Thr Ala Thr Ala Thr Ala Gly Pro Leu Pro Val
85 90 95
Gly Ser Val Arg Glu Phe Arg Val Leu Ser Gly Leu Pro Gly Thr Ser
100 105 110
Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser
115 120 125
Phe Arg Val Val Gly Gly Glu His Arg Leu Ser Asn Tyr Arg Ser Val
130 135 140
Thr Thr Val His Glu Thr Ala Ala Gly Ala Ala Ala Ala Val Val Val
145 150 155 160
Glu Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala Asp Glu Thr
165 170 175
Arg Met Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Ala
180 185 190
Arg Thr Ala Glu Gln Leu Ala Leu Ala Ala Pro Arg Ala Ala
195 200 205
<210> 85
<211> 396
<212> PRT
<213> 葡萄(Vitis vinifera)
<220>
<223> 酿酒葡萄黑皮诺假设蛋白, 克隆
ENTAV 115, 基因座标签VITISV_001710, GenBank登录号
CAN76441.1
<220>
<221> 变体
<222> (1)...(396)
<223> Xaa = 任何氨基酸
<400> 85
Met Pro Ile Ser Ser Leu Pro Phe Ser Leu Tyr Thr Val Thr Pro Asn
1 5 10 15
Pro Leu Lys Leu Ile Thr Thr His Ala His Ala Phe Thr Pro His Thr
20 25 30
His Ile Phe Thr Leu Lys Phe Met Ser His Thr Tyr Cys Pro His Ile
35 40 45
His His Ile Thr Ser Ile His Tyr Thr His Leu Leu Xaa Pro Ile Pro
50 55 60
His Met Pro Leu Gln Pro Pro Leu Pro Pro His Pro Ile Leu Pro Ser
65 70 75 80
Met Pro Ala Phe Gln His Leu Tyr Ser Thr Asn Gln His Leu Gln Val
85 90 95
Ala Leu Phe Ser Ala Arg Gly Pro Asn Ile Arg Asp Phe Asn Phe Gln
100 105 110
Asp Ala Asp Leu Leu Lys Leu Asp Ile Leu Ala Pro Gly Ser Leu Ile
115 120 125
Trp Ala Ala Trp Ser Pro Asn Gly Thr Asp Glu Ala Asn Tyr Val Gly
130 135 140
Glu Gly Ser Pro Thr Val Ala Met Ile Ala Lys Arg Gly Pro Arg His
145 150 155 160
Gly Lys Tyr Met Ala Phe Cys Xaa Met Tyr Arg Asp Asn Val Ala Pro
165 170 175
Lys Gly Val Asn Xaa Ala Val Ala Thr Val Lys Thr Lys Arg Thr Ile
180 185 190
Gln Leu Lys Thr Ser Leu Glu Ile Ala Cys His Tyr Ala Gly Ile Asn
195 200 205
Ile Ser Gly Ile Asn Gly Glu Val Met Pro Gly Gln Trp Glu Tyr Gln
210 215 220
Val Gly Pro Gly Gln Cys Ser Ser Leu Leu Ala Gln Arg Val His Val
225 230 235 240
Pro Leu Ser Ala Val Gly Ser Val Val His Arg Phe Asp Lys Pro Gln
245 250 255
Arg Tyr Gln His Val Ile Lys Ser Cys Arg Ile Glu Asp Gly Phe Glu
260 265 270
Met Arg Met Gly Xaa Leu Arg Asp Val Asn Ile Ile Ser Gly Leu Pro
275 280 285
Thr Ala Thr Asn Thr Gly Arg Leu Asp Met Gln Asp Asp Glu Arg His
290 295 300
Val Thr Arg Cys Pro His Gln Arg Gln Ser Glu Ser Lys Tyr Thr Glu
305 310 315 320
Asn Asn Asn Ser Asp Ala Ser Ser Ile Lys Ser Pro Ile Asn Gly Pro
325 330 335
Ser Glu His Leu Lys Thr Ala Ala Ser Pro Lys Thr Glu Ser Ile Ile
340 345 350
Val Ile Asp Thr Ser Lys Phe Leu Asn Glu Glu Asp Phe Glu Gly Lys
355 360 365
Asp Glu Thr Ser Ser Ser Asn Gln Val Gln Ile Glu Asp Glu Asn Trp
370 375 380
Glu Thr Arg Phe Pro Asn Thr Asp Ala Gly Ile Trp
385 390 395
<210> 86
<211> 443
<212> PRT
<213> 葡萄(Vitis vinifera)
<220>
<223> 酿酒葡萄黑皮诺假设蛋白, 克隆
ENTAV 115, 基因座标签VITISV_014403, GenBank登录号
CAN9881.1
<220>
<221> 变体
<222> (1)...(443)
<223> Xaa = 任何氨基酸
<400> 86
Met Pro Ser Ala Xaa Lys Ser Ser Thr Val Pro Leu Ser Leu Xaa Gln
1 5 10 15
Phe Lys Leu Gly Leu Arg His Gly His Arg Val Ile Pro Trp Gly Asp
20 25 30
Leu Asp Ser Leu Ala Met Leu Gln Arg Gln Leu Asp Val Asp Ile Leu
35 40 45
Val Thr Gly His Thr His Arg Phe Thr Ala Tyr Lys His Glu Gly Gly
50 55 60
Val Val Ile Asn Pro Gly Ser Ala Thr Gly Ala Phe Gly Ser Ile Thr
65 70 75 80
Tyr Asp Val Asn Pro Ser Phe Val Leu Met Asp Ile Asp Gly Leu Arg
85 90 95
Val Val Val Cys Val Tyr Glu Leu Ile Asp Glu Thr Ala Asn Ile Ile
100 105 110
Lys Glu Leu His Ala Arg Lys Ile Ser Phe Gly Thr Lys Ser Met Ile
115 120 125
Xaa Cys Leu Leu Leu Lys Arg Arg Ser Thr Pro Lys Phe Arg Arg Lys
130 135 140
Lys Leu Phe Leu Phe Gln Cys Arg Val Gln Met Thr Leu Thr Leu Thr
145 150 155 160
Asn Leu Ala Val Ser Gly Ile Ala Gln Thr Leu Gln Val Asp Gln Trp
165 170 175
Thr Val Cys Ala Leu Ile Phe Met Thr Arg Arg Asp Ile His Leu Asp
180 185 190
Lys Ala Arg Phe Leu Asp Phe Lys Asp Met Gly Lys Leu Leu Ala Asp
195 200 205
Ala Ser Gly Leu Arg Lys Ala Leu Ser Gly Gly Xaa Val Thr Ala Gly
210 215 220
Met Ala Ile Phe Asp Thr Met Arg His Ile Arg Pro Asp Val Pro Thr
225 230 235 240
Val Cys Val Gly Leu Ala Ala Val Ala Met Ile Ala Lys Arg Gly Pro
245 250 255
Arg His Gly Lys Tyr Met Ala Phe Cys Pro Met Tyr Arg Asp Asn Val
260 265 270
Ala Pro Lys Gly Val Asn Val Ala Val Val Thr Val Lys Thr Lys Arg
275 280 285
Thr Ile Gln Leu Lys Thr Ser Leu Glu Ile Ala Cys His Tyr Ala Gly
290 295 300
Ile Asn Ile Ser Gly Ile Asn Gly Glu Val Met Pro Gly Gln Trp Glu
305 310 315 320
Tyr Gln Val Gly Pro Gly Gln Cys Ser Ser Leu Leu Ala Gln Arg Val
325 330 335
His Val Pro Leu Ser Ala Val Gly Ser Val Val His Arg Phe Asp Lys
340 345 350
Pro Gln Arg Tyr Gln His Val Ile Lys Ser Cys Arg Ile Glu Asp Gly
355 360 365
Phe Glu Met Arg Met Gly Arg Leu Arg Asp Val Asn Ile Ile Ser Gly
370 375 380
Leu Pro Thr Ala Thr Asn Thr Gly Arg Leu Asp Met Gln Asp Asp Glu
385 390 395 400
Xaa His Val Thr Arg Cys Pro His Gln Arg Gln Ser Glu Ser Lys Tyr
405 410 415
Thr Glu Asn Asn Asn Ser Asp Ala Ser Ser Val Lys Ser Pro Ile Asn
420 425 430
Gly Pro Ser Glu His Leu Lys Thr Ala Ala Xaa
435 440
<210> 87
<211> 95
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻籼组, 品种Pokkali, capip1蛋白, 克隆
OSR-385-428-D5, GenBank登录号ABR25904.1
<400> 87
Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys Thr Gly Leu Pro Ala
1 5 10 15
Thr Thr Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asp Glu His Ile
20 25 30
Leu Ser Val Lys Phe Val Gly Gly Asp His Arg Leu Arg Asn Tyr Ser
35 40 45
Ser Ile Val Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly Thr
50 55 60
Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr Lys
65 70 75 80
Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
85 90 95
<210> 88
<211> 191
<212> PRT
<213> 玉米(Zea mays)
<220>
<223> 玉米株B73 未知蛋白, 克隆 ZM_BFc0034O07,
GenBank登录号ACF84624.1
<400> 88
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Ala Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Pro Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Phe Val Gly Gly Asp His Arg
130 135 140
Leu Gln Val Cys Ser Val Leu His Leu Ser Ile Phe Cys Ala Ala His
145 150 155 160
Ala Arg Tyr Phe Ala His His Leu Lys Cys Val Leu Glu Phe Leu Cys
165 170 175
Gln Met His Leu Asp Val Leu Pro Cys Asp Asp Ala Ile Leu Glu
180 185 190
<210> 89
<211> 239
<212> PRT
<213> 稻(Oryza sativa)
<220>
<223> 稻粳组, 日本晴品种, 假设
蛋白, 基因座标签OsJ_020681, GenBank登录号
EAZ37198.1
<400> 89
Met Asn Gly Cys Thr Gly Gly Ala Gly Gly Val Ala Ala Gly Arg Leu
1 5 10 15
Pro Ala Val Ser Leu Gln Gln Ala Gln Trp Lys Leu Val Asp Glu Arg
20 25 30
Cys Glu Leu Arg Glu Glu Glu Met Glu Tyr Val Arg Arg Phe His Arg
35 40 45
His Glu Ile Gly Ser Asn Gln Cys Asn Ser Phe Ile Ala Lys His Val
50 55 60
Arg Ala Pro Leu Gln Asn Val Trp Ser Leu Val Arg Arg Phe Asp Gln
65 70 75 80
Pro Gln Ile Tyr Lys Pro Phe Val Arg Lys Cys Val Met Arg Gly Asn
85 90 95
Val Glu Thr Gly Ser Val Arg Glu Ile Ile Val Gln Ser Gly Leu Pro
100 105 110
Ala Thr Arg Ser Ile Glu Arg Leu Glu Phe Leu Asp Asp Asn Glu Tyr
115 120 125
Ile Leu Arg Val Lys Phe Ile Gly Gly Asp His Met Leu Lys Lys Arg
130 135 140
Ile Pro Lys Lys Thr Tyr Ala Ile Ser Ser Arg Thr Cys Ser Asp Ser
145 150 155 160
Ala Ile Ile Ala Val Gly Gln Ser Asn Cys Ala Pro Glu Ile Thr Ala
165 170 175
Met Asn Gly Gly Val Ser Ile Gln Pro Trp Leu Ile Leu Leu Ala Phe
180 185 190
Phe Ser Ser Pro Ser Asn Gln Thr Asn Pro Asp Ser Leu Arg Asp Met
195 200 205
His Pro Gly Ser Trp Phe Gln Ile Leu Leu Val Leu Ala Met Phe Thr
210 215 220
Cys Ser Lys Gly Ser Val Leu Pro Pro Ser Glu Lys Val Asn Val
225 230 235
<210> 90
<211> 188
<212> PRT
<213> 玉米(Zea mays)
<220>
<223> 玉米GRMZM2G154987_P01蛋白
<400> 90
Met Glu Pro His Met Glu Ser Ala Leu Arg Gln Gly Leu Ser Glu Ala
1 5 10 15
Glu Gln Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro
20 25 30
Gly Arg Ala Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp
35 40 45
Ala Pro Leu Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro
50 55 60
Gln Arg Tyr Lys His Phe Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp
65 70 75 80
Gly Ala Thr Val Gly Ser Val Arg Glu Val Thr Val Val Ser Gly Leu
85 90 95
Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg
100 105 110
His Ile Leu Ser Phe Arg Val Val Gly Gly Asp His Arg Leu Arg Asn
115 120 125
Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys
130 135 140
Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu
145 150 155 160
Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln
165 170 175
Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn
180 185
<210> 91
<211> 205
<212> PRT
<213> 玉米(Zea mays)
<220>
<223> 玉米GRMZM2G134731_P01蛋白
<400> 91
Met Asp Gln Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Pro Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Val
65 70 75 80
Arg Pro Asp Pro Asp Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg
85 90 95
Glu Val Cys Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp His Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Gly Pro Gly Ile Cys Thr Val Val Leu Glu Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Ser Thr Ser Ser Ser Ala Pro Pro Pro Pro Ser Glu
195 200 205
<210> 92
<211> 220
<212> PRT
<213> 玉米(Zea mays)
<220>
<223> 玉米GRMZM2G144224_P01蛋白
<400> 92
Met Pro Cys Ile Gln Ala Ser Ser Pro Gly Gly Met Pro His Gln His
1 5 10 15
Gly Arg Gly Arg Val Leu Gly Gly Gly Val Gly Cys Ala Ala Glu Val
20 25 30
Ala Ala Ala Val Ala Ala Ser Ala Gly Gly Met Arg Cys Gly Ala His
35 40 45
Asp Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala
50 55 60
Ala Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val Ala Ala
65 70 75 80
Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Val Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly
100 105 110
Gly Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala
115 120 125
Ala Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val
130 135 140
Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Arg Asn Tyr Leu
145 150 155 160
Ser Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr
165 170 175
Val Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro
180 185 190
Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln
195 200 205
Ser Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
210 215 220
<210> 93
<211> 221
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma01g02290.1蛋白
<400> 93
Met Glu Lys Ala Glu Ser Ser Ala Ser Thr Ser Glu Pro Asp Ser Asp
1 5 10 15
Glu Asn His His Arg His Pro Thr Asn His His Ile Asn Pro Pro Ser
20 25 30
Gly Leu Thr Pro Leu Glu Phe Ala Ser Leu Ile Pro Ser Val Ala Glu
35 40 45
His His Ser Tyr Leu Val Gly Ser Gly Gln Cys Ser Ser Leu Leu Ala
50 55 60
Gln Arg Val Gln Ala Pro Pro Asp Ala Val Trp Ser Val Val Arg Arg
65 70 75 80
Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser Cys Ala Val
85 90 95
Lys Glu Pro Phe His Met Ala Val Gly Val Thr Arg Asp Val Asn Val
100 105 110
Ile Ser Gly Leu Pro Ala Ala Thr Ser Thr Glu Arg Leu Asp Leu Leu
115 120 125
Asp Asp Ile Arg Cys Val Thr Gly Phe Ser Ile Ile Gly Gly Glu His
130 135 140
Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val His Ser Phe Glu Asp
145 150 155 160
Asp Ala Asp Asp Gly Lys Ile Tyr Thr Val Val Leu Glu Ser Tyr Val
165 170 175
Val Asp Val Pro Asp Gly Asn Thr Glu Glu Asp Thr Arg Leu Phe Ala
180 185 190
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Ser Val Thr Glu
195 200 205
Gly Thr Asn Arg Asp Gly Asp Gly Lys Ser His Ser Arg
210 215 220
<210> 94
<211> 214
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma01g12970.1蛋白
<400> 94
Met Glu Lys Thr His Ser Ser Ser Ala Glu Glu Gln Asp Pro Thr Arg
1 5 10 15
Arg His Leu Asp Pro Pro Pro Gly Leu Thr Ala Glu Glu Phe Glu Asp
20 25 30
Leu Lys Pro Ser Val Leu Glu His His Thr Tyr Ser Val Thr Pro Thr
35 40 45
Arg Gln Ser Ser Ser Leu Leu Ala Gln Arg Ile His Ala Pro Pro His
50 55 60
Ala Val Trp Ser Val Val Arg Cys Phe Asp Asn Pro Gln Ala Tyr Lys
65 70 75 80
His Phe Ile Lys Ser Cys His Val Lys Glu Gly Phe Gln Leu Ala Val
85 90 95
Gly Ser Thr Arg Asp Val His Val Ile Ser Gly Leu Pro Ala Ala Thr
100 105 110
Ser Thr Glu Arg Leu Asp Leu Leu Asp Asp Asp Arg His Val Ile Gly
115 120 125
Phe Thr Ile Val Gly Gly Asp His Arg Leu Arg Asn Tyr Arg Ser Val
130 135 140
Thr Ser Val His Gly Phe Glu Cys Asp Gly Lys Ile Trp Thr Val Val
145 150 155 160
Leu Glu Ser Tyr Val Val Asp Val Pro Glu Gly Asn Thr Glu Glu Asp
165 170 175
Thr Arg Leu Phe Ala Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu
180 185 190
Ala Ser Val Ser Glu Gly Met Cys Gly Asp Gly Asp Gly Asp Gly Asp
195 200 205
Gly Lys Gly Asn Lys Ser
210
<210> 95
<211> 216
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma01g31320.1蛋白
<400> 95
Met Leu Gln Asn Ser Ser Met Ser Ser Leu Leu Leu His Arg Ile Asn
1 5 10 15
Gly Gly Gly Gly Ala Thr Thr Ala Thr Asn Cys His Asp Thr Val Phe
20 25 30
Met Thr Val Pro Asp Gly Val Ala Arg Tyr His Thr His Ala Val Ala
35 40 45
Pro Asn Gln Cys Cys Ser Ser Val Ala Gln Glu Ile Gly Ala Ser Val
50 55 60
Ala Thr Val Trp Ser Val Leu Arg Arg Phe Asp Asn Pro Gln Ala Tyr
65 70 75 80
Lys His Phe Val Lys Ser Cys His Val Ile Gly Gly Asp Gly Asp Val
85 90 95
Gly Thr Leu Arg Glu Val His Val Ile Ser Gly Leu Pro Ala Ala Arg
100 105 110
Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg His Val Ile Ser
115 120 125
Phe Ser Val Val Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser Val
130 135 140
Thr Thr Leu His Pro Thr Ala Ser Ser Ala Ser Gly Gly Cys Ser Gly
145 150 155 160
Thr Val Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr
165 170 175
Arg Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu
180 185 190
Gln Ser Leu Ala Gln Thr Ala Glu Asn Leu Thr Leu Arg Lys Asn Asn
195 200 205
Asn Asn Asp Tyr Lys Cys Cys Ser
210 215
<210> 96
<211> 208
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma02g42990.1蛋白
<400> 96
Met Thr Ser Leu Gln Phe His Arg Phe Asn Pro Ala Thr Asp Thr Ser
1 5 10 15
Thr Ala Ile Ala Asn Gly Val Asn Cys Pro Lys Pro Pro Ser Thr Leu
20 25 30
Arg Leu Leu Ala Lys Val Ser Leu Ser Val Pro Glu Thr Val Ala Arg
35 40 45
His His Ala His Pro Val Gly Pro Asn Gln Cys Cys Ser Val Val Ile
50 55 60
Gln Ala Ile Asp Ala Pro Val Ser Ala Val Trp Pro Val Val Arg Arg
65 70 75 80
Phe Asp Asn Pro Gln Ala Tyr Lys His Phe Val Lys Ser Cys His Val
85 90 95
Val Ala Ala Ala Gly Gly Gly Glu Asp Gly Ile Arg Val Gly Ala Leu
100 105 110
Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Val Ser Ser Thr Glu
115 120 125
Arg Leu Glu Ile Leu Asp Asp Glu Arg His Val Met Ser Phe Ser Val
130 135 140
Val Gly Gly Asp His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Leu
145 150 155 160
His Gly Asp Gly Asn Gly Gly Thr Val Val Ile Glu Ser Tyr Val Val
165 170 175
Asp Val Pro Pro Gly Asn Thr Lys Glu Glu Thr Cys Val Phe Val Asp
180 185 190
Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Ala Gln Ile Ala Glu Thr
195 200 205
<210> 97
<211> 176
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma04g05380.1蛋白
<400> 97
Ala Tyr Pro Val Leu Gly Leu Thr Pro Glu Glu Phe Ser Glu Leu Glu
1 5 10 15
Ser Ile Ile Asn Thr His His Lys Phe Glu Pro Ser Pro Glu Ile Cys
20 25 30
Ser Ser Ile Ile Ala Gln Arg Ile Asp Ala Pro Ala His Thr Val Trp
35 40 45
Pro Leu Val Arg Ser Phe Glu Asn Pro Gln Lys Tyr Lys His Phe Val
50 55 60
Lys Ser Cys Asn Met Arg Ser Gly Asp Gly Gly Val Gly Ser Ile Arg
65 70 75 80
Glu Val Thr Val Val Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
85 90 95
Leu Glu Ile Leu Asp Asp Asp Lys His Leu Leu Ser Phe Arg Val Val
100 105 110
Gly Gly Glu His Arg Leu His Asn Tyr Arg Ser Val Thr Ser Val Asn
115 120 125
Glu Phe Lys Asn Pro Asp Asn Gly Lys Val Tyr Thr Ile Val Leu Glu
130 135 140
Ser Tyr Val Val Asp Ile Pro Glu Gly Asn Thr Gly Val Asp Thr Lys
145 150 155 160
Met Phe Val Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Gly Glu
165 170 175
<210> 98
<211> 172
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma06g05440.1蛋白
<400> 98
Glu Phe Thr Glu Leu Glu Ser Thr Ile Asn Thr His His Lys Phe Glu
1 5 10 15
Ala Ser Pro Glu Ile Cys Ser Ser Ile Ile Ala Gln Arg Ile Asp Ala
20 25 30
Pro Ala His Thr Val Trp Pro Leu Val Arg Ser Phe Glu Asn Pro Gln
35 40 45
Lys Tyr Lys His Phe Val Lys Ser Cys Asn Met Arg Ser Gly Asp Gly
50 55 60
Gly Val Gly Ser Ile Arg Glu Val Thr Val Val Ser Gly Leu Pro Ala
65 70 75 80
Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp Asp Asn His Leu
85 90 95
Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu His Asn Tyr Arg
100 105 110
Ser Val Thr Ser Val Asn Glu Phe Lys Arg Pro Asp Asn Gly Lys Val
115 120 125
Tyr Thr Ile Val Leu Glu Ser Tyr Val Val Asp Ile Pro Glu Gly Asn
130 135 140
Thr Gly Val Asp Thr Lys Met Phe Val Asp Thr Val Val Lys Leu Asn
145 150 155 160
Leu Gln Lys Leu Gly Glu Val Ala Met Ala Thr Asn
165 170
<210> 99
<211> 191
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma06g13150.1蛋白
<400> 99
Met Thr Glu Leu Ser Ser Arg Glu Val Glu Tyr Ile Arg Arg His His
1 5 10 15
Ser Lys Ala Ala Glu Asp Asn Gln Cys Ala Ser Ala Leu Val Lys His
20 25 30
Ile Arg Ala Pro Leu Pro Leu Val Trp Ser Leu Val Arg Arg Phe Asp
35 40 45
Glu Pro Gln Lys Tyr Lys Pro Phe Val Ser Arg Cys Val Val Arg Gly
50 55 60
Asn Leu Glu Ile Gly Ser Leu Arg Glu Val Asp Val Lys Ser Gly Leu
65 70 75 80
Pro Ala Thr Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp Asn His
85 90 95
His Ile Leu Ser Val Arg Ile Ile Gly Gly Asp His Arg Leu Arg Asn
100 105 110
Tyr Ser Ser Ile Met Ser Leu His Pro Glu Ile Val Asp Gly Arg Pro
115 120 125
Gly Thr Leu Val Ile Glu Ser Phe Val Val Asp Ile Pro Glu Gly Asn
130 135 140
Thr Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Leu Ile Lys Cys Asn
145 150 155 160
Leu Lys Ser Leu Ala Asp Val Ser Glu Gly Leu Thr Leu Gln Asp His
165 170 175
Thr Glu Pro Ile Asp Arg Lys Tyr Glu Leu Leu Ile Thr Arg Gly
180 185 190
<210> 100
<211> 185
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma07g06270.1蛋白
<400> 100
Met Asn Gly Gly Glu Ser Tyr Gly Ala Ile Glu Thr Gln Tyr Ile Arg
1 5 10 15
Arg His His Lys His Glu Pro Arg Glu Asn Gln Cys Thr Ser Ala Leu
20 25 30
Val Lys His Ile Arg Ala Pro Val His Leu Val Trp Ser Leu Val Arg
35 40 45
Arg Phe Asp Gln Pro Gln Lys Tyr Lys Pro Phe Val Ser Arg Cys Ile
50 55 60
Met Gln Gly Asp Leu Gly Ile Gly Ser Val Arg Glu Val Asn Val Lys
65 70 75 80
Ser Gly Leu Pro Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp
85 90 95
Asp Glu Glu His Ile Leu Gly Ile Arg Ile Val Gly Gly Asp His Arg
100 105 110
Leu Arg Asn Tyr Ser Ser Ile Ile Thr Val His Pro Glu Val Ile Asp
115 120 125
Gly Arg Pro Gly Thr Met Val Ile Glu Ser Phe Val Val Asp Val Pro
130 135 140
Asp Gly Asn Thr Arg Asp Glu Thr Cys Tyr Phe Val Glu Ala Leu Ile
145 150 155 160
Arg Cys Asn Leu Ser Ser Leu Ala Asp Val Ser Glu Arg Met Ala Val
165 170 175
Gln Gly Arg Thr Asn Pro Ile Asn His
180 185
<210> 101
<211> 178
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma07g19120.1蛋白
<400> 101
Met Ser Pro Asn Asn Pro Ser Thr Ile Val Ser Asp Ala Val Ala Arg
1 5 10 15
His His Thr His Val Val Ser Pro His Gln Cys Cys Ser Ala Val Val
20 25 30
Gln Glu Ile Ala Ala Pro Val Ser Thr Val Trp Ser Val Val Arg Arg
35 40 45
Phe Asp Asn Pro Gln Ala Tyr Lys His Phe Val Lys Ser Cys His Val
50 55 60
Ile Leu Gly Asp Gly Asp Val Gly Thr Leu Arg Glu Val Arg Val Ile
65 70 75 80
Ser Gly Leu Pro Ala Ala Val Ser Thr Glu Arg Leu Asp Val Leu Asp
85 90 95
Asp Glu Arg His Val Ile Gly Phe Ser Met Val Gly Gly Asp His Arg
100 105 110
Leu Ser Asn Tyr Arg Ser Val Thr Ile Leu His Pro Arg Ser Ala Thr
115 120 125
Asp Thr Val Val Val Glu Ser Tyr Val Val Asp Val Pro Ala Gly Asn
130 135 140
Thr Thr Glu Asp Thr Arg Val Phe Val Asp Thr Ile Leu Arg Cys Asn
145 150 155 160
Leu Gln Ser Leu Ala Lys Phe Ala Glu Asn Leu Thr Asn Lys Leu His
165 170 175
Gln Arg
<210> 102
<211> 246
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma08g36770.1蛋白
<400> 102
Met Ser Arg Ser His Asn Lys Arg Lys Pro Phe Ser Phe Ile Phe Lys
1 5 10 15
Ile Thr Leu Leu Glu Leu Leu Ser Ser Leu Leu Ser Ser Ser Leu Arg
20 25 30
Phe Ala Met Asp Lys Thr His Ser Gly Glu Glu Gln Asp Pro Asn Pro
35 40 45
Thr His Pro Thr Arg Asn His Leu Asp Pro Pro Pro Gly Leu Thr Pro
50 55 60
Glu Glu Phe Glu Asp Leu Lys Pro Ser Val Leu Glu His His Thr Tyr
65 70 75 80
Ser Val Thr Pro Thr Arg Gln Cys Ser Ser Leu Leu Ala Gln Arg Ile
85 90 95
His Ala Pro Pro His Thr Val Trp Thr Val Val Arg Cys Phe Asp Asn
100 105 110
Pro Gln Ala Tyr Lys His Phe Ile Lys Ser Cys His Val Lys Glu Gly
115 120 125
Phe Gln Leu Ala Val Gly Ser Thr Arg Asp Val His Val Ile Ser Gly
130 135 140
Leu Pro Ala Ala Thr Ser Thr Glu Arg Leu Asp Leu Leu Asp Asp Asp
145 150 155 160
Arg His Val Ile Gly Phe Thr Ile Val Gly Gly Asp His Arg Leu Arg
165 170 175
Asn Tyr Arg Ser Val Thr Ser Val His Gly Phe Glu Arg Asp Gly Lys
180 185 190
Ile Trp Thr Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Glu Gly
195 200 205
Asn Thr Glu Glu Asp Thr Arg Leu Phe Ala Asp Thr Val Val Lys Leu
210 215 220
Asn Leu Gln Lys Leu Ala Ser Val Thr Glu Gly Met Cys Gly Asp Ser
225 230 235 240
Asp Gly Lys Gly Asn Asn
245
<210> 103
<211> 223
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma09g33700.1蛋白
<400> 103
Met Glu Lys Ala Glu Ser Ser Ala Ser Thr Ser Glu Pro Asp Ser Asp
1 5 10 15
Asp Asn His His Arg His Pro Thr Asn His His Leu Asn Pro Pro Ser
20 25 30
Gly Leu Thr Pro Leu Glu Phe Ala Ser Leu Val Pro Ser Val Ala Glu
35 40 45
His His Ser Tyr Leu Val Gly Pro Gly Gln Cys Ser Ser Leu Leu Ala
50 55 60
Gln Arg Val His Ala Pro Pro Asp Ala Val Trp Ser Phe Val Arg Arg
65 70 75 80
Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser Cys Ala Val
85 90 95
Lys Glu Pro Phe His Met Ala Val Gly Val Thr Arg Asp Val Asn Val
100 105 110
Ile Ser Gly Leu Pro Ala Ala Thr Ser Thr Glu Arg Leu Asp Phe Leu
115 120 125
Asp Asp Val Arg Arg Val Thr Gly Phe Ser Ile Ile Gly Gly Glu His
130 135 140
Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val His Ser Phe Asp Asp
145 150 155 160
Asp Asn Ala Ser Ala Asp Gly Lys Ile Tyr Thr Val Val Leu Glu Ser
165 170 175
Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu Glu Asp Thr Arg Leu
180 185 190
Phe Ala Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Ser Val
195 200 205
Thr Glu Gly Thr Asn Gly Asp Gly Asp Gly Lys Pro His Ser Arg
210 215 220
<210> 104
<211> 229
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma11g35670.1蛋白
<400> 104
Met Pro Ser Ser Leu His Phe Asp Arg Phe Asn Pro Ile Thr His Ala
1 5 10 15
Ala Thr Thr Val Ala Ile Ala Asn Gly Val Asn Cys Pro Lys Gln Pro
20 25 30
Gln Ala Pro Pro Ser Ser Thr Ala Ala Arg Arg Leu Val Val Pro Ser
35 40 45
Leu Ser Ser Gly Arg Gly Ile Ala Ala Pro Asp Thr Val Ala Leu His
50 55 60
His Ala His Val Val Asp Pro Asn Gln Cys Cys Ser Ile Val Thr Gln
65 70 75 80
His Ile Asn Ala Pro Val Ser Ala Val Trp Ala Val Val Arg Arg Phe
85 90 95
Asp Asn Pro Gln Gly Tyr Lys Asn Phe Val Arg Ser Cys His Val Ile
100 105 110
Thr Gly Asp Gly Ile Arg Val Gly Ala Val Arg Glu Val Arg Val Val
115 120 125
Ser Gly Leu Pro Ala Glu Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp
130 135 140
Asp Glu Arg His Val Ile Ser Phe Ser Met Val Gly Gly Asp His Arg
145 150 155 160
Leu Arg Asn Tyr Gln Ser Val Thr Thr Leu His Ala Asn Gly Asn Gly
165 170 175
Thr Leu Val Ile Glu Ser Tyr Val Val Asp Val Pro Gln Gly Asn Thr
180 185 190
Lys Glu Glu Thr Cys Val Phe Val Asp Thr Ile Val Arg Cys Asn Leu
195 200 205
Gln Ser Leu Ala Gln Ile Ala Glu Asn Arg Thr Asn Asn Cys Glu His
210 215 220
Thr Ala Gln His Cys
225
<210> 105
<211> 191
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma13g08120.1蛋白
<400> 105
Met Asn Gly Ile Gly Asn Asp Gly Gly Gly Gly Leu Ser Asn Val Glu
1 5 10 15
Met Glu Tyr Ile Arg Arg His His Arg His Glu Pro Gly Glu Asn Gln
20 25 30
Cys Gly Ser Ala Leu Val Lys His Ile Arg Ala Pro Val Pro Gln Val
35 40 45
Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Lys Tyr Lys Pro Phe
50 55 60
Val Ser Arg Cys Val Val Arg Gly Asn Leu Glu Ile Gly Ser Leu Arg
65 70 75 80
Glu Val Asp Val Lys Ser Gly Leu Pro Ala Thr Thr Ser Thr Glu Arg
85 90 95
Leu Glu Leu Leu Asp Asp Asn Glu His Leu Leu Ser Ile Arg Ile Ile
100 105 110
Gly Gly Asp His Arg Leu Arg Asn Tyr Ser Ser Ile Met Ser Leu His
115 120 125
Pro Glu Ile Ile Asp Gly Arg Pro Gly Thr Leu Val Ile Glu Ser Phe
130 135 140
Val Val Asp Val Pro Glu Gly Asn Thr Lys Asp Glu Thr Cys Tyr Phe
145 150 155 160
Val Glu Ala Leu Ile Lys Cys Asn Leu Lys Ser Leu Ala Asp Val Ser
165 170 175
Glu Gly Ile Ala Val Gln Asp Arg Thr Glu Pro Ile Asp Arg Ile
180 185 190
<210> 106
<211> 169
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma14g06100.1蛋白
<400> 106
Met Val Ala Arg His His Ala His Ala Val Gly Pro Asn Gln Cys Cys
1 5 10 15
Ser Phe Val Ile Gln Ala Ile Asp Ala Pro Val Ser Ala Val Trp Pro
20 25 30
Val Val Arg Arg Phe Asp Asn Pro Gln Ala Tyr Lys His Phe Val Lys
35 40 45
Ser Cys His Val Val Ala Ala Gly Gly Ala Gly Gly Asp Gly Gly Ile
50 55 60
His Val Gly Ala Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala
65 70 75 80
Val Ser Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg His Val
85 90 95
Met Ser Phe Ser Val Val Gly Gly Asp His Arg Leu Arg Asn Tyr Arg
100 105 110
Ser Val Thr Thr Leu His Gly Asp Gly Ser Asn Gly Gly Thr Val Val
115 120 125
Ile Glu Ser Tyr Val Val Asp Ile Pro Ala Gly Asn Thr Lys Glu Glu
130 135 140
Thr Cys Val Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
145 150 155 160
Ala Gln Met Ala Glu Asn Met Gly Ser
165
<210> 107
<211> 210
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma14g10730.1蛋白
<400> 107
Met Thr Ile Leu Pro His Ser Asn Asn Lys Ser Ser Asn His Lys Phe
1 5 10 15
Ile Ala His Gln Asn Tyr Met Ala Ser Glu Thr His His His Val Gln
20 25 30
Gly Leu Thr Pro Glu Glu Leu Thr Lys Leu Glu Pro Ile Ile Lys Lys
35 40 45
Tyr His Leu Phe Glu Gln Ser Pro Asn Thr Cys Phe Ser Ile Ile Thr
50 55 60
Tyr Arg Ile Glu Ala Pro Ala Lys Ala Val Trp Pro Phe Val Arg Ser
65 70 75 80
Phe Asp Asn Pro Gln Lys Tyr Lys His Phe Ile Lys Gly Cys Asn Met
85 90 95
Arg Gly Asp Gly Gly Val Gly Ser Ile Arg Glu Val Thr Val Val Ser
100 105 110
Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp
115 120 125
Asp Lys His Val Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu
130 135 140
Lys Asn Tyr Arg Ser Val Thr Ser Val Asn Glu Phe Asn Lys Glu Gly
145 150 155 160
Lys Val Tyr Thr Ile Val Leu Glu Ser Tyr Ile Val Asp Ile Pro Glu
165 170 175
Gly Asn Thr Glu Glu Asp Thr Lys Met Phe Val Asp Thr Val Val Lys
180 185 190
Leu Asn Leu Gln Lys Leu Gly Val Val Ala Met Ala Ser Ser Met His
195 200 205
Gly Gln
210
<210> 108
<211> 193
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma14g30260.1蛋白
<400> 108
Met Asn Arg Ile Gly Asn Gly Gly Gly Gly Gly Gly Gly Leu Ser Asn
1 5 10 15
Val Glu Met Glu Tyr Ile Arg Arg His His Arg His Glu Pro Gly Glu
20 25 30
Asn Gln Cys Gly Ser Ala Leu Val Lys His Ile Arg Ala Pro Val Pro
35 40 45
Gln Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Lys Tyr Lys
50 55 60
Pro Phe Ile Ser Arg Cys Val Val Arg Gly Asn Leu Glu Ile Gly Ser
65 70 75 80
Leu Arg Glu Val Asp Val Lys Ser Gly Leu Pro Ala Thr Thr Ser Thr
85 90 95
Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile Leu Ser Ile Arg
100 105 110
Ile Ile Gly Gly Asp His Arg Leu Arg Asn Tyr Ser Ser Ile Met Ser
115 120 125
Leu His Pro Glu Ile Ile Asp Gly Arg Pro Gly Thr Leu Val Ile Glu
130 135 140
Ser Phe Val Val Asp Val Pro Glu Gly Asn Thr Lys Asp Glu Thr Cys
145 150 155 160
Tyr Phe Val Glu Ala Leu Ile Lys Cys Asn Leu Lys Ser Leu Ala Asp
165 170 175
Val Ser Glu Gly Leu Ala Val Gln Asp Cys Thr Glu Pro Ile Asp Arg
180 185 190
Ile
<210> 109
<211> 188
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma17g34800.1蛋白
<400> 109
Met Ala Ser Glu Thr His His His Val Gln Gly Leu Thr Pro Glu Glu
1 5 10 15
Leu Thr Gln Leu Glu Pro Ile Ile Lys Lys Tyr His Leu Phe Glu Ala
20 25 30
Ser Ser Asn Lys Cys Phe Ser Ile Ile Thr His Arg Ile Glu Ala Pro
35 40 45
Ala Ser Ser Val Trp Pro Leu Val Arg Asn Phe Asp Asn Pro Gln Lys
50 55 60
Tyr Lys His Phe Ile Lys Gly Cys Asn Met Lys Gly Asp Gly Ser Val
65 70 75 80
Gly Ser Ile Arg Glu Val Thr Val Val Ser Gly Leu Pro Ala Ser Thr
85 90 95
Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp Asp Lys His Val Leu Ser
100 105 110
Phe Arg Val Val Gly Gly Glu His Arg Leu Gln Asn Tyr Arg Ser Val
115 120 125
Thr Ser Val Asn Glu Phe His Lys Glu Gly Lys Val Tyr Thr Ile Val
130 135 140
Leu Glu Ser Tyr Ile Val Asp Ile Pro Glu Gly Asn Thr Glu Glu Asp
145 150 155 160
Thr Lys Met Phe Val Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu
165 170 175
Gly Val Val Ala Met Ala Ser Ser Met Asn Gly Arg
180 185
<210> 110
<211> 177
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma18g43680.1蛋白
<400> 110
Met Leu Pro Asn Asn Pro Ser Thr Ile Val Pro Asp Ala Val Ala Arg
1 5 10 15
His His Thr His Val Val Ser Pro Gln Gln Cys Cys Ser Ala Val Val
20 25 30
Gln Glu Ile Ala Ala Pro Val Ser Thr Val Trp Ser Val Val Arg Arg
35 40 45
Phe Asp Asn Pro Gln Ala Tyr Lys His Phe Val Lys Ser Cys His Val
50 55 60
Ile Leu Gly Asp Gly Asp Val Gly Thr Leu Arg Glu Val His Val Ile
65 70 75 80
Ser Gly Leu Pro Ala Ala Val Ser Thr Glu Arg Leu Asp Val Leu Asp
85 90 95
Asp Glu Arg His Val Ile Gly Phe Ser Met Val Gly Gly Asp His Arg
100 105 110
Leu Phe Asn Tyr Arg Ser Val Thr Thr Leu His Pro Arg Ser Ala Ala
115 120 125
Gly Thr Val Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly Asn
130 135 140
Thr Thr Glu Asp Thr Arg Val Phe Val Asp Thr Ile Leu Arg Cys Asn
145 150 155 160
Leu Gln Ser Leu Ala Lys Phe Ala Glu Asn Leu Thr Lys Leu His Gln
165 170 175
Arg
<210> 111
<211> 185
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma07g06270.2蛋白
<400> 111
Met Asn Gly Gly Glu Ser Tyr Gly Ala Ile Glu Thr Gln Tyr Ile Arg
1 5 10 15
Arg His His Lys His Glu Pro Arg Glu Asn Gln Cys Thr Ser Ala Leu
20 25 30
Val Lys His Ile Arg Ala Pro Val His Leu Val Trp Ser Leu Val Arg
35 40 45
Arg Phe Asp Gln Pro Gln Lys Tyr Lys Pro Phe Val Ser Arg Cys Ile
50 55 60
Met Gln Gly Asp Leu Gly Ile Gly Ser Val Arg Glu Val Asn Val Lys
65 70 75 80
Ser Gly Leu Pro Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp
85 90 95
Asp Glu Glu His Ile Leu Gly Ile Arg Ile Val Gly Gly Asp His Arg
100 105 110
Leu Arg Asn Tyr Ser Ser Ile Ile Thr Val His Pro Glu Val Ile Asp
115 120 125
Gly Arg Pro Gly Thr Met Val Ile Glu Ser Phe Val Val Asp Val Pro
130 135 140
Asp Gly Asn Thr Arg Asp Glu Thr Cys Tyr Phe Val Glu Ala Leu Ile
145 150 155 160
Arg Cys Asn Leu Ser Ser Leu Ala Asp Val Ser Glu Arg Met Ala Val
165 170 175
Gln Gly Arg Thr Asn Pro Ile Asn His
180 185
<210> 112
<211> 191
<212> PRT
<213> 橹豆(Glycine max)
<220>
<223> 大豆Glyma16g02910.1蛋白
<400> 112
Met Gly Ile Thr Ile Gly Ile Gln Cys Leu Glu Ile Glu Glu Ile Ser
1 5 10 15
Ile Cys Asp Gly Met Phe Cys Tyr Leu Val Asp Phe Val Asp Val Lys
20 25 30
Glu Lys Met Asn Tyr Cys Leu Met Trp Phe Gly Tyr Phe Pro Ser Gln
35 40 45
Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Lys Tyr Lys Pro
50 55 60
Phe Val Ser Arg Cys Ile Met Gln Gly Asp Leu Gly Ile Gly Ser Val
65 70 75 80
Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala Thr Thr Ser Thr Glu
85 90 95
Arg Leu Glu Gln Leu Asp Asp Glu Glu His Ile Leu Gly Ile Arg Ile
100 105 110
Val Gly Gly Asp His Arg Leu Arg Asn Tyr Ser Ser Ile Ile Thr Val
115 120 125
His Pro Glu Val Ile Asp Gly Arg Pro Ser Thr Met Val Ile Glu Ser
130 135 140
Phe Val Val Asp Val Pro Asp Gly Asn Thr Arg Asp Glu Thr Cys Tyr
145 150 155 160
Phe Val Glu Ala Leu Ile Arg Cys Asn Leu Ser Ser Leu Ala Asp Val
165 170 175
Ser Glu Arg Met Ala Val Gln Gly Arg Thr Asp Pro Ile Asn His
180 185 190
<210> 113
<211> 185
<212> PRT
<213> 人工序列
<220>
<223> 合成PYR/PYL受体蛋白
<400> 113
Met Asn Gly Gly Glu Ser Tyr Gly Ala Ile Glu Thr Gln Tyr Ile Arg
1 5 10 15
Arg His His Lys His Glu Pro Arg Glu Asn Gln Cys Thr Ser Ala Leu
20 25 30
Val Lys His Ile Arg Ala Pro Val His Leu Val Trp Ser Leu Val Arg
35 40 45
Arg Phe Asp Gln Pro Gln Lys Tyr Lys Pro Phe Val Ser Arg Cys Ile
50 55 60
Met Gln Gly Asp Leu Gly Ile Gly Ser Val Arg Glu Val Asn Val Lys
65 70 75 80
Ser Gly Leu Pro Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp
85 90 95
Asp Glu Glu His Ile Leu Gly Ile Arg Ile Val Gly Gly Asp His Arg
100 105 110
Leu Arg Asn Tyr Ser Ser Ile Ile Thr Val His Pro Glu Val Ile Asp
115 120 125
Gly Arg Pro Ser Thr Met Val Ile Glu Ser Phe Val Val Asp Val Pro
130 135 140
Asp Gly Asn Thr Arg Asp Glu Thr Cys Tyr Phe Val Glu Ala Leu Ile
145 150 155 160
Arg Cys Asn Leu Ser Ser Leu Ala Asp Val Ser Glu Arg Met Ala Val
165 170 175
Gln Gly Arg Thr Asp Pro Ile Asn His
180 185
<210> 114
<211> 204
<212> PRT
<213> 高粱(Sorghum bicolor)
<220>
<223> 高粱Sb10g022200蛋白
<400> 114
Met Glu Thr His Val Glu Arg Ala Leu Arg Ala Thr Leu Thr Glu Ala
1 5 10 15
Glu Val Arg Ala Leu Glu Pro Ala Val Arg Glu His His Thr Phe Pro
20 25 30
Ala Gly Arg Val Ala Ala Gly Thr Thr Thr Pro Thr Pro Thr Thr Cys
35 40 45
Thr Ser Leu Val Ala Gln Arg Val Ser Ala Pro Val Arg Ala Val Trp
50 55 60
Pro Ile Val Arg Ser Phe Gly Asn Pro Gln Arg Tyr Lys His Phe Val
65 70 75 80
Arg Thr Cys Ala Leu Ala Ala Gly Asp Gly Ala Ser Val Gly Ser Val
85 90 95
Arg Glu Val Thr Val Val Ser Gly Leu Pro Ala Ser Ser Ser Thr Glu
100 105 110
Arg Leu Glu Val Leu Asp Asp Asp Arg His Ile Leu Ser Phe Arg Val
115 120 125
Val Gly Gly Asp His Arg Leu Arg Asn Tyr Arg Ser Val Thr Ser Val
130 135 140
Thr Glu Phe Gln Pro Gly Pro Tyr Cys Val Val Val Glu Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Glu Gly Asn Thr Ala Glu Asp Thr Arg Met Phe Thr
165 170 175
Asp Thr Val Val Arg Leu Asn Leu Gln Lys Leu Ala Ala Val Ala Glu
180 185 190
Glu Ser Ala Ala Ala Ala Ala Ala Gly Asn Arg Arg
195 200
<210> 115
<211> 204
<212> PRT
<213> 高粱(Sorghum bicolor)
<220>
<223> 高粱Sb04g008040蛋白
<400> 115
Met Glu Pro His Met Glu Thr Ala Leu Arg Gln Gly Gly Leu Ser Glu
1 5 10 15
Leu Glu Gln Arg Glu Leu Glu Pro Val Val Arg Ala His His Thr Phe
20 25 30
Pro Gly Arg Ser Pro Gly Thr Thr Cys Thr Ser Leu Val Thr Gln Arg
35 40 45
Val Asp Ala Pro Leu Ser Ala Val Trp Pro Ile Val Arg Gly Phe Ala
50 55 60
Ala Pro Gln Arg Tyr Lys His Phe Ile Lys Ser Cys Asp Leu Arg Ser
65 70 75 80
Gly Asp Gly Ala Thr Val Gly Ser Val Arg Glu Val Thr Val Val Ser
85 90 95
Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp
100 105 110
Asp Arg His Ile Leu Ser Phe Arg Val Val Gly Gly Asp His Arg Leu
115 120 125
Arg Asn Tyr Arg Ser Val Thr Ser Val Thr Glu Phe His His His His
130 135 140
Gln Ala Ala Ala Gly Arg Pro Tyr Cys Val Val Val Glu Ser Tyr Val
145 150 155 160
Val Asp Val Pro Glu Gly Asn Thr Glu Glu Asp Thr Arg Met Phe Thr
165 170 175
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Ala Ile Ala Thr
180 185 190
Ser Ser Ala Ala Ala Ala Ala Ser Asn Ser Ser Thr
195 200
<210> 116
<211> 258
<212> PRT
<213> 高粱(Sorghum bicolor)
<220>
<223> 高粱Sb01g028330蛋白
<400> 116
Met Val Glu Ser Pro Asn Pro Asn Ser Pro Ser Arg Pro Leu Cys Ile
1 5 10 15
Lys Tyr Thr Arg Ala Pro Ala Arg His Phe Ser Pro Pro Leu Pro Phe
20 25 30
Ser Ser Leu Ile Ile Ser Ala Asn Pro Ile Glu Pro Lys Ala Met Asp
35 40 45
Lys Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu Gly Leu
50 55 60
Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala His His
65 70 75 80
Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala Gln Arg
85 90 95
Ile Gln Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg Phe Asp
100 105 110
Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Leu Arg Pro
115 120 125
Asp Pro Glu Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg Glu Val
130 135 140
Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg Leu Asp
145 150 155 160
Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr Gly Gly
165 170 175
Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser Glu Leu
180 185 190
Ala Asp Pro Gly Ile Cys Thr Val Val Leu Glu Ser Tyr Val Val Asp
195 200 205
Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala Asp Thr
210 215 220
Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu Ala Asn
225 230 235 240
Ala Ala Ala Ala Ala Ser Phe Val Ser Val Val Pro Pro Pro Glu Pro
245 250 255
Glu Glu
<210> 117
<211> 222
<212> PRT
<213> 高粱(Sorghum bicolor)
<220>
<223> 高粱Sb01g038150蛋白
<400> 117
Met Pro Cys Leu Gln Ala Ser Ser Ser Pro Gly Ser Met Pro His Gln
1 5 10 15
His His Gly Arg Val Leu Ala Gly Val Gly Cys Ala Ala Glu Val Ala
20 25 30
Ala Ala Ala Val Ala Ala Thr Ser Pro Ala Ala Gly Met Arg Cys Gly
35 40 45
Ala His Asp Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His
50 55 60
Ala Ala Pro Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val
65 70 75 80
Ala Ala Pro Ala Ser Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln
85 90 95
Pro Gln Ala Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala Gly
100 105 110
Asp Gly Gly Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly Leu
115 120 125
Pro Ala Ala Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser
130 135 140
His Val Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Gln Asn
145 150 155 160
Tyr Leu Ser Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala
165 170 175
Ala Thr Val Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly Asn
180 185 190
Thr Pro Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn
195 200 205
Leu Gln Ser Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
210 215 220
<210> 118
<211> 211
<212> PRT
<213> 高粱(Sorghum bicolor)
<220>
<223> 高粱Sb04g009280蛋白
<400> 118
Met Val Glu Met Asp Gly Gly Val Gly Val Val Gly Gly Gly Gln Gln
1 5 10 15
Thr Pro Ala Pro Arg Arg Trp Arg Leu Ala Asp Glu Leu Arg Cys Asp
20 25 30
Leu Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His Glu
35 40 45
Pro Arg Asp His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys Ala
50 55 60
Pro Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln
65 70 75 80
Leu Phe Lys Pro Phe Val Ser Arg Cys Glu Met Lys Gly Asn Ile Glu
85 90 95
Ile Gly Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala Thr
100 105 110
Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile Leu
115 120 125
Ser Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser Ser
130 135 140
Ile Leu Thr Val His Pro Glu Val Ile Asp Gly Arg Pro Gly Thr Leu
145 150 155 160
Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr Lys Asp
165 170 175
Glu Thr Cys Tyr Phe Val Glu Ala Leu Leu Lys Cys Asn Leu Lys Ser
180 185 190
Leu Ala Glu Val Ser Glu Arg Gln Val Ile Lys Asp Gln Thr Glu Pro
195 200 205
Leu Asp Arg
210
<210> 119
<211> 216
<212> PRT
<213> 高粱(Sorghum bicolor)
<220>
<223> 高粱Sb09g023180蛋白
<400> 119
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Thr Gly Gly Gly Ala Lys Ala Ala Ile Val Ala Ala Ser His Gly Ala
20 25 30
Ser Cys Ala Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala
35 40 45
Ala Arg Ala Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala
50 55 60
Pro Val Gly Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln
65 70 75 80
Ala Tyr Lys His Phe Ile Arg Ser Cys Arg Leu Val Asp Asp Gly Gly
85 90 95
Gly Gly Ala Gly Ala Gly Ala Gly Ala Thr Val Ala Val Gly Ser Val
100 105 110
Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Thr Ser Ser Arg Glu
115 120 125
Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser Phe Arg Val
130 135 140
Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr Val
145 150 155 160
His Glu Ala Glu Ala Gly Ala Gly Gly Thr Val Val Val Glu Ser Tyr
165 170 175
Val Val Asp Val Pro Pro Gly Asn Thr Ala Asp Glu Thr Arg Val Phe
180 185 190
Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Ala Arg Thr Ala
195 200 205
Glu Arg Leu Ala Leu Ala Leu Ala
210 215
<210> 120
<211> 36
<212> PRT
<213> 人工序列
<220>
<223> 合成超敏PYR/PYL受体共有序列
<220>
<221> 变体
<222> (1)...(36)
<223> Xaa = 任何氨基酸
<400> 120
Cys Xaa Ser Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ala Pro Xaa Xaa Xaa Xaa
1 5 10 15
Trp Xaa Xaa Xaa Xaa Xaa Phe Xaa Xaa Pro Xaa Xaa Xaa Xaa Xaa Phe
20 25 30
Xaa Xaa Xaa Cys
35
<210> 121
<211> 25
<212> PRT
<213> 人工序列
<220>
<223> 合成超敏PYR/PYL受体共有序列
<220>
<221> 变体
<222> (1)...(25)
<223> Xaa = 任何氨基酸
<400> 121
Gly Xaa Xaa Arg Xaa Val Xaa Xaa Xaa Ser Xaa Xaa Pro Ala Xaa Xaa
1 5 10 15
Ser Xaa Glu Xaa Leu Xaa Xaa Xaa Asp
20 25
<210> 122
<211> 11
<212> PRT
<213> 人工序列
<220>
<223> 合成超敏PYR/PYL受体共有序列
<220>
<221> 变体
<222> (1)...(11)
<223> Xaa = 任何氨基酸
<400> 122
Gly Gly Xaa His Arg Leu Xaa Asn Tyr Xaa Ser
1 5 10
<210> 123
<211> 31
<212> PRT
<213> 人工序列
<220>
<223> 合成超敏PYR/PYL受体共有序列
<220>
<221> 变体
<222> (1)...(31)
<223> Xaa = 任何氨基酸
<400> 123
Glu Ser Xaa Xaa Val Asp Xaa Pro Xaa Gly Xaa Xaa Xaa Xaa Xaa Thr
1 5 10 15
Xaa Xaa Phe Xaa Xaa Xaa Xaa Xaa Xaa Xaa Asn Leu Xaa Xaa Leu
20 25 30
<210> 124
<211> 191
<212> PRT
<213> 人工序列
<220>
<223> 合成拟南芥PYR1突变F61L
<400> 124
Met Pro Ser Glu Leu Thr Pro Glu Glu Arg Ser Glu Leu Lys Asn Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Leu Val Trp Ser Ile
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Leu Ile Lys Ser
50 55 60
Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Val Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val Leu Glu Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Ala
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Ala Glu
165 170 175
Ala Met Ala Arg Asn Ser Gly Asp Gly Ser Gly Ser Gln Val Thr
180 185 190
<210> 125
<211> 191
<212> PRT
<213> 人工序列
<220>
<223> 合成拟南芥PYR1突变F61M
<400> 125
Met Pro Ser Glu Leu Thr Pro Glu Glu Arg Ser Glu Leu Lys Asn Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Leu Val Trp Ser Ile
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Met Ile Lys Ser
50 55 60
Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Val Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val Leu Glu Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Ala
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Ala Glu
165 170 175
Ala Met Ala Arg Asn Ser Gly Asp Gly Ser Gly Ser Gln Val Thr
180 185 190
<210> 126
<211> 191
<212> PRT
<213> 人工序列
<220>
<223> 合成拟南芥PYR1突变V81I
<400> 126
Met Pro Ser Glu Leu Thr Pro Glu Glu Arg Ser Glu Leu Lys Asn Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Leu Val Trp Ser Ile
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser
50 55 60
Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Ile Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val Leu Glu Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Ala
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Ala Glu
165 170 175
Ala Met Ala Arg Asn Ser Gly Asp Gly Ser Gly Ser Gln Val Thr
180 185 190
<210> 127
<211> 191
<212> PRT
<213> 人工序列
<220>
<223> 合成拟南芥PYR1突变V81Y
<400> 127
Met Pro Ser Glu Leu Thr Pro Glu Glu Arg Ser Glu Leu Lys Asn Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Leu Val Trp Ser Ile
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser
50 55 60
Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Tyr Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val Leu Glu Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Ala
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Ala Glu
165 170 175
Ala Met Ala Arg Asn Ser Gly Asp Gly Ser Gly Ser Gln Val Thr
180 185 190
<210> 128
<211> 191
<212> PRT
<213> 人工序列
<220>
<223> 合成拟南芥PYR1突变I110C
<400> 128
Met Pro Ser Glu Leu Thr Pro Glu Glu Arg Ser Glu Leu Lys Asn Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Leu Val Trp Ser Ile
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser
50 55 60
Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Val Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Cys Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val Leu Glu Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Ala
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Ala Glu
165 170 175
Ala Met Ala Arg Asn Ser Gly Asp Gly Ser Gly Ser Gln Val Thr
180 185 190
<210> 129
<211> 191
<212> PRT
<213> 人工序列
<220>
<223> 合成拟南芥PYR1突变I110S
<400> 129
Met Pro Ser Glu Leu Thr Pro Glu Glu Arg Ser Glu Leu Lys Asn Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Leu Val Trp Ser Ile
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser
50 55 60
Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Val Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ser Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val Leu Glu Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Ala
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Ala Glu
165 170 175
Ala Met Ala Arg Asn Ser Gly Asp Gly Ser Gly Ser Gln Val Thr
180 185 190
<210> 130
<211> 191
<212> PRT
<213> 人工序列
<220>
<223> 合成拟南芥PYR1突变E141C
<400> 130
Met Pro Ser Glu Leu Thr Pro Glu Glu Arg Ser Glu Leu Lys Asn Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Leu Val Trp Ser Ile
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser
50 55 60
Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Val Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val Leu Cys Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Ala
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Ala Glu
165 170 175
Ala Met Ala Arg Asn Ser Gly Asp Gly Ser Gly Ser Gln Val Thr
180 185 190
<210> 131
<211> 191
<212> PRT
<213> 人工序列
<220>
<223> 合成拟南芥PYR1突变E141I
<400> 131
Met Pro Ser Glu Leu Thr Pro Glu Glu Arg Ser Glu Leu Lys Asn Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Leu Val Trp Ser Ile
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser
50 55 60
Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Val Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val Leu Ile Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Ala
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Ala Glu
165 170 175
Ala Met Ala Arg Asn Ser Gly Asp Gly Ser Gly Ser Gln Val Thr
180 185 190
<210> 132
<211> 191
<212> PRT
<213> 人工序列
<220>
<223> 合成拟南芥PYR1突变E141L
<400> 132
Met Pro Ser Glu Leu Thr Pro Glu Glu Arg Ser Glu Leu Lys Asn Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Leu Val Trp Ser Ile
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser
50 55 60
Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Val Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val Leu Leu Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Ala
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Ala Glu
165 170 175
Ala Met Ala Arg Asn Ser Gly Asp Gly Ser Gly Ser Gln Val Thr
180 185 190
<210> 133
<211> 191
<212> PRT
<213> 人工序列
<220>
<223> 合成拟南芥PYR1突变E141M
<400> 133
Met Pro Ser Glu Leu Thr Pro Glu Glu Arg Ser Glu Leu Lys Asn Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Leu Val Trp Ser Ile
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser
50 55 60
Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Val Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val Leu Met Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Ala
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Ala Glu
165 170 175
Ala Met Ala Arg Asn Ser Gly Asp Gly Ser Gly Ser Gln Val Thr
180 185 190
<210> 134
<211> 191
<212> PRT
<213> 人工序列
<220>
<223> 合成拟南芥PYR1突变E141N
<400> 134
Met Pro Ser Glu Leu Thr Pro Glu Glu Arg Ser Glu Leu Lys Asn Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Leu Val Trp Ser Ile
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser
50 55 60
Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Val Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val Leu Asn Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Ala
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Ala Glu
165 170 175
Ala Met Ala Arg Asn Ser Gly Asp Gly Ser Gly Ser Gln Val Thr
180 185 190
<210> 135
<211> 191
<212> PRT
<213> 人工序列
<220>
<223> 合成拟南芥PYR1突变E141T
<400> 135
Met Pro Ser Glu Leu Thr Pro Glu Glu Arg Ser Glu Leu Lys Asn Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Leu Val Trp Ser Ile
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser
50 55 60
Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Val Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val Leu Thr Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Ala
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Ala Glu
165 170 175
Ala Met Ala Arg Asn Ser Gly Asp Gly Ser Gly Ser Gln Val Thr
180 185 190
<210> 136
<211> 191
<212> PRT
<213> 人工序列
<220>
<223> 合成拟南芥PYR1突变E141V
<400> 136
Met Pro Ser Glu Leu Thr Pro Glu Glu Arg Ser Glu Leu Lys Asn Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Leu Val Trp Ser Ile
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser
50 55 60
Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Val Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val Leu Val Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Ala
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Ala Glu
165 170 175
Ala Met Ala Arg Asn Ser Gly Asp Gly Ser Gly Ser Gln Val Thr
180 185 190
<210> 137
<211> 191
<212> PRT
<213> 人工序列
<220>
<223> 合成拟南芥PYR1突变E141W
<400> 137
Met Pro Ser Glu Leu Thr Pro Glu Glu Arg Ser Glu Leu Lys Asn Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Leu Val Trp Ser Ile
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser
50 55 60
Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Val Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val Leu Trp Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Ala
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Ala Glu
165 170 175
Ala Met Ala Arg Asn Ser Gly Asp Gly Ser Gly Ser Gln Val Thr
180 185 190
<210> 138
<211> 191
<212> PRT
<213> 人工序列
<220>
<223> 合成拟南芥PYR1突变E141Y
<400> 138
Met Pro Ser Glu Leu Thr Pro Glu Glu Arg Ser Glu Leu Lys Asn Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Leu Val Trp Ser Ile
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser
50 55 60
Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Val Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val Leu Tyr Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Ala
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Ala Glu
165 170 175
Ala Met Ala Arg Asn Ser Gly Asp Gly Ser Gly Ser Gln Val Thr
180 185 190
<210> 139
<211> 191
<212> PRT
<213> 人工序列
<220>
<223> 合成拟南芥PYR1突变A160C
<400> 139
Met Pro Ser Glu Leu Thr Pro Glu Glu Arg Ser Glu Leu Lys Asn Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Leu Val Trp Ser Ile
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser
50 55 60
Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Val Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val Leu Glu Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Cys
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Ala Glu
165 170 175
Ala Met Ala Arg Asn Ser Gly Asp Gly Ser Gly Ser Gln Val Thr
180 185 190
<210> 140
<211> 191
<212> PRT
<213> 人工序列
<220>
<223> 合成拟南芥PYR1突变A160I
<400> 140
Met Pro Ser Glu Leu Thr Pro Glu Glu Arg Ser Glu Leu Lys Asn Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Leu Val Trp Ser Ile
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser
50 55 60
Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Val Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val Leu Glu Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Ile
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Ala Glu
165 170 175
Ala Met Ala Arg Asn Ser Gly Asp Gly Ser Gly Ser Gln Val Thr
180 185 190
<210> 141
<211> 191
<212> PRT
<213> 人工序列
<220>
<223> 合成拟南芥PYR1突变A160V
<400> 141
Met Pro Ser Glu Leu Thr Pro Glu Glu Arg Ser Glu Leu Lys Asn Ser
1 5 10 15
Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro Gly Ser Cys Ser Ser
20 25 30
Leu His Ala Gln Arg Ile His Ala Pro Pro Glu Leu Val Trp Ser Ile
35 40 45
Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys His Phe Ile Lys Ser
50 55 60
Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val Gly Cys Thr Arg Asp
65 70 75 80
Val Ile Val Ile Ser Gly Leu Pro Ala Asn Thr Ser Thr Glu Arg Leu
85 90 95
Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly Phe Ser Ile Ile Gly
100 105 110
Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val Thr Thr Val His Arg
115 120 125
Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val Leu Glu Ser Tyr Val
130 135 140
Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp Thr Arg Met Phe Val
145 150 155 160
Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala Thr Val Ala Glu
165 170 175
Ala Met Ala Arg Asn Ser Gly Asp Gly Ser Gly Ser Gln Val Thr
180 185 190
<210> 142
<211> 174
<212> PRT
<213> 人工序列
<220>
<223> 玉米(Zea mays)的合成中间部分 PYR/PYL (ZmPYLc)
<400> 142
Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala
1 5 10 15
Ala Gly Gln Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala Pro Val
20 25 30
Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Arg Tyr
35 40 45
Lys Arg Phe Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Ala Glu
50 55 60
Val Gly Ser Val Arg Glu Leu Leu Leu Val Ser Gly Leu Pro Ala Glu
65 70 75 80
Ser Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile
85 90 95
Ser Phe Arg Val Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser
100 105 110
Val Thr Thr Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg Pro Leu
115 120 125
Thr Met Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr
130 135 140
Val Glu Glu Thr Arg Ile Phe Val Asp Thr Ile Val Arg Cys Asn Leu
145 150 155 160
Gln Ser Leu Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala
165 170
<210> 143
<211> 172
<212> PRT
<213> 人工序列
<220>
<223> 玉米(Zea mays)的合成中间部分 PYR/PYL (ZmPYLl)
<400> 143
Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala
1 5 10 15
Ala Gly Gln Cys Cys Ser Val Met Val Gln Ser Ile Ala Ala Pro Ala
20 25 30
Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Gly Tyr
35 40 45
Lys Arg Phe Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Val Glu
50 55 60
Val Gly Ser Val Arg Glu Leu Leu Val Val Ser Gly Leu Pro Ala Glu
65 70 75 80
Asn Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile
85 90 95
Ser Phe Arg Ile Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser
100 105 110
Val Thr Thr Val His Glu Ala Ala Ser Glu Gly Gly Pro Leu Thr Met
115 120 125
Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Val Glu
130 135 140
Glu Thr Arg Ile Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser
145 150 155 160
Leu Glu Asp Thr Val Ile Arg Gln Gln Ala Met Ala
165 170
<210> 144
<211> 170
<212> PRT
<213> 人工序列
<220>
<223> 玉米(Zea mays)的合成中间部分 PYR/PYL (ZmPYLa)
<400> 144
Cys Ala Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala Ala
1 5 10 15
Arg Ala Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala Pro
20 25 30
Val Gly Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala
35 40 45
Tyr Lys His Phe Ile Arg Ser Cys Arg Leu Val Gly Gly Gly Asp Val
50 55 60
Ala Val Gly Ser Val Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala
65 70 75 80
Thr Ser Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val
85 90 95
Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg
100 105 110
Ser Val Thr Thr Val His Glu Ala Gly Ala Gly Ala Gly Thr Gly Thr
115 120 125
Val Val Val Glu Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala
130 135 140
Asp Glu Thr Arg Val Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln
145 150 155 160
Ser Leu Ala Arg Thr Ala Glu Arg Leu Ala
165 170
<210> 145
<211> 171
<212> PRT
<213> 人工序列
<220>
<223> 玉米(Zea mays)的合成中间部分 PYR/PYL (ZmPYLb)
<400> 145
Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Ala
1 5 10 15
Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val Ala Ala Pro
20 25 30
Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Val
35 40 45
Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly
50 55 60
Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Ala
65 70 75 80
Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu
85 90 95
Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Arg Asn Tyr Leu Ser
100 105 110
Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val
115 120 125
Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu
130 135 140
Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln Ser
145 150 155 160
Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
165 170
<210> 146
<211> 172
<212> PRT
<213> 人工序列
<220>
<223> 玉米(Zea mays)的合成中间部分 PYR/PYL (ZmPYLo)
<400> 146
Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Pro
1 5 10 15
Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln Arg Val Ala Ala Pro
20 25 30
Ala Glu Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Ala
35 40 45
Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly
50 55 60
Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Ala
65 70 75 80
Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu
85 90 95
Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Gln Asn Tyr Leu Ser
100 105 110
Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val
115 120 125
Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu
130 135 140
Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln Ser
145 150 155 160
Leu Ala Thr Thr Ala Glu Lys Leu Ala Leu Ala Ala
165 170
<210> 147
<211> 174
<212> PRT
<213> 人工序列
<220>
<223> 玉米(Zea mays)的合成中间部分 PYR/PYL (ZmPYLi)
<400> 147
Gln Leu Arg Ser Thr Val Asp Ala His His Arg Tyr Ala Val Gly Glu
1 5 10 15
Gly Gln Cys Ser Ser Leu Leu Ala Gln Arg Ile His Ala Pro Pro Glu
20 25 30
Ala Val Trp Ala Val Val Arg Arg Phe Asp Cys Pro Gln Val Tyr Lys
35 40 45
His Phe Ile Arg Ser Cys Ala Leu Arg Pro Asp Pro Glu Ala Gly Asp
50 55 60
Ala Leu Cys Pro Gly Arg Leu Arg Glu Val Ser Val Ile Ser Gly Leu
65 70 75 80
Pro Ala Ser Thr Ser Thr Glu Arg Leu Asp Leu Leu Asp Asp Ala Ala
85 90 95
Arg Val Phe Gly Phe Ser Ile Thr Gly Gly Glu His Arg Leu Arg Asn
100 105 110
Tyr Arg Ser Val Thr Thr Val Ser Glu Leu Ala Asp Pro Ala Ile Cys
115 120 125
Thr Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr
130 135 140
Glu Asp Asp Thr Arg Leu Phe Ala Asp Thr Val Ile Arg Leu Asn Leu
145 150 155 160
Gln Lys Leu Lys Ser Val Ala Glu Ala Asn Ala Ala Glu Ala
165 170
<210> 148
<211> 174
<212> PRT
<213> 人工序列
<220>
<223> 玉米(Zea mays)的合成中间部分 PYR/PYL (ZmPYLk)
<400> 148
Gln Leu Arg Pro Thr Val Asp Ala His His Arg Tyr Ala Val Gly Glu
1 5 10 15
Gly Gln Cys Ser Ser Leu Leu Ala Gln Arg Ile His Ala Pro Pro Ala
20 25 30
Ala Val Trp Ala Ile Val Arg Arg Phe Asp Cys Pro Gln Val Tyr Lys
35 40 45
His Phe Ile Arg Ser Cys Ala Val Arg Pro Asp Pro Asp Ala Gly Asp
50 55 60
Ala Leu Arg Pro Gly Arg Leu Arg Glu Val Cys Val Ile Ser Gly Leu
65 70 75 80
Pro Ala Ser Thr Ser Thr Glu Arg Leu Asp His Leu Asp Asp Ala Ala
85 90 95
Arg Val Phe Gly Phe Ser Ile Thr Gly Gly Glu His Arg Leu Arg Asn
100 105 110
Tyr Arg Ser Val Thr Thr Val Ser Glu Leu Ala Gly Pro Gly Ile Cys
115 120 125
Thr Val Val Leu Glu Ser Tyr Ala Val Asp Val Pro Asp Gly Asn Thr
130 135 140
Glu Asp Asp Thr Arg Leu Phe Ala Asp Thr Val Ile Arg Leu Asn Leu
145 150 155 160
Gln Lys Leu Lys Ser Val Ala Glu Ala Ser Thr Ser Ser Ser
165 170
<210> 149
<211> 171
<212> PRT
<213> 人工序列
<220>
<223> 拟南芥(Arabidopsis thaliana)的合成中间部分 PYR/PYL
(AtPyrl)
<400> 149
Glu Leu Lys Asn Ser Ile Ala Glu Phe His Thr Tyr Gln Leu Asp Pro
1 5 10 15
Gly Ser Cys Ser Ser Leu His Ala Gln Arg Ile His Ala Pro Pro Glu
20 25 30
Leu Val Trp Ser Ile Val Arg Arg Phe Asp Lys Pro Gln Thr Tyr Lys
35 40 45
His Phe Ile Lys Ser Cys Ser Val Glu Gln Asn Phe Glu Met Arg Val
50 55 60
Gly Cys Thr Arg Asp Val Ile Val Ile Ser Gly Leu Pro Ala Asn Thr
65 70 75 80
Ser Thr Glu Arg Leu Asp Ile Leu Asp Asp Glu Arg Arg Val Thr Gly
85 90 95
Phe Ser Ile Ile Gly Gly Glu His Arg Leu Thr Asn Tyr Lys Ser Val
100 105 110
Thr Thr Val His Arg Phe Glu Lys Glu Asn Arg Ile Trp Thr Val Val
115 120 125
Leu Glu Ser Tyr Val Val Asp Met Pro Glu Gly Asn Ser Glu Asp Asp
130 135 140
Thr Arg Met Phe Ala Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu
145 150 155 160
Ala Thr Val Ala Glu Ala Met Ala Arg Asn Ser
165 170
<210> 150
<211> 169
<212> PRT
<213> 人工序列
<220>
<223> 玉米(Zea mays)的合成中间部分 PYR/PYL (ZmPYLj)
<400> 150
Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro Gly Arg Ala
1 5 10 15
Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp Ala Pro Leu
20 25 30
Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro Gln Arg Tyr
35 40 45
Lys His Phe Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp Gly Ala Thr
50 55 60
Val Gly Ser Val Arg Glu Val Thr Val Val Ser Gly Leu Pro Ala Ser
65 70 75 80
Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg His Ile Leu
85 90 95
Ser Phe Arg Val Val Gly Gly Asp His Arg Leu Arg Asn Tyr Arg Ser
100 105 110
Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys Val Val Leu
115 120 125
Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu Glu Asp Thr
130 135 140
Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu Ala
145 150 155 160
Ala Ile Ala Thr Ser Ser Ser Ala Asn
165
<210> 151
<211> 171
<212> PRT
<213> 人工序列
<220>
<223> 玉米(Zea mays)的合成中间部分 PYR/PYL (ZmPYLd)
<400> 151
Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His Glu Pro
1 5 10 15
Arg Asp His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys Ala Pro
20 25 30
Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Leu
35 40 45
Phe Lys Pro Phe Val Ser Arg Cys Glu Met Lys Gly Asn Ile Glu Ile
50 55 60
Gly Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala Thr Arg
65 70 75 80
Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asp Glu Arg Ile Leu Ser
85 90 95
Val Arg Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser Ser Ile
100 105 110
Leu Thr Val His Pro Glu Val Ile Asp Gly Arg Pro Gly Thr Leu Val
115 120 125
Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr Lys Asp Glu
130 135 140
Thr Cys Tyr Phe Val Glu Ala Leu Leu Lys Cys Asn Leu Arg Ser Leu
145 150 155 160
Ala Glu Val Ser Glu Gly Gln Val Ile Met Asp
165 170
<210> 152
<211> 171
<212> PRT
<213> 人工序列
<220>
<223> 玉米(Zea mays)的合成中间部分 PYR/PYL (ZmPYLf)
<400> 152
Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His Glu Pro
1 5 10 15
Arg Glu His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys Ala Pro
20 25 30
Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Leu
35 40 45
Phe Lys Pro Phe Val Ser Arg Cys Glu Met Lys Gly Asn Ile Glu Ile
50 55 60
Gly Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala Thr Arg
65 70 75 80
Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile Leu Ser
85 90 95
Val Arg Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser Ser Ile
100 105 110
Leu Thr Val His Pro Glu Val Ile Asp Gly Arg Pro Gly Thr Leu Val
115 120 125
Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr Lys Asp Glu
130 135 140
Thr Cys Tyr Phe Val Glu Ala Leu Leu Lys Cys Asn Leu Lys Ser Leu
145 150 155 160
Ala Glu Val Ser Glu Arg Gln Val Val Lys Asp
165 170
<210> 153
<211> 172
<212> PRT
<213> 人工序列
<220>
<223> 玉米(Zea mays)的合成中间部分 PYR/PYL (ZmPYLe)
<400> 153
Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His Ala Pro
1 5 10 15
Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys Ala Pro
20 25 30
Val His Leu Val Trp Glu Leu Val Arg Arg Phe Asp Gln Pro Gln Arg
35 40 45
Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln Leu Glu
50 55 60
Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro Ala Thr
65 70 75 80
Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His Ile Leu
85 90 95
Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser Ser
100 105 110
Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly Thr Leu
115 120 125
Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr Lys Asp
130 135 140
Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu Asn Ser
145 150 155 160
Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser
165 170
<210> 154
<211> 172
<212> PRT
<213> 人工序列
<220>
<223> 玉米(Zea mays)的合成中间部分 PYR/PYL (ZmPYLg)
<400> 154
Ala Gly Gly Glu Thr Glu Tyr Val Arg Arg Leu His Arg His Ala Pro
1 5 10 15
Ala Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys Ala Pro
20 25 30
Val His Leu Val Trp Glu Leu Val Arg Ser Phe Asp Gln Pro Gln Arg
35 40 45
Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln Leu Glu
50 55 60
Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro Ala Thr
65 70 75 80
Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His Ile Leu
85 90 95
Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser Ser
100 105 110
Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly Thr Leu
115 120 125
Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr Lys Asp
130 135 140
Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu Lys Ser
145 150 155 160
Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser
165 170
<210> 155
<211> 233
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLc，同源于AtPYR1 A160C
<400> 155
Met Arg Glu Arg Asn Ser Ser Ile Asp Gln Glu His Gln Arg Gly Ser
1 5 10 15
Ser Ser Arg Ser Thr Met Pro Phe Ala Ala Ser Arg Thr Ser Gln Gln
20 25 30
Gln His Ser Arg Val Ala Thr Asn Gly Arg Ala Val Ala Val Cys Ala
35 40 45
Gly His Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala
50 55 60
Val Ala Ala Gly Gln Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala
65 70 75 80
Pro Val Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Arg Tyr Lys Arg Phe Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly
100 105 110
Ala Glu Val Gly Ser Val Arg Glu Leu Leu Leu Val Ser Gly Leu Pro
115 120 125
Ala Glu Ser Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg
130 135 140
Val Ile Ser Phe Arg Val Leu Gly Gly Asp His Arg Leu Ala Asn Tyr
145 150 155 160
Arg Ser Val Thr Thr Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg
165 170 175
Pro Leu Thr Met Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Val Glu Glu Thr Arg Ile Phe Cys Asp Thr Ile Val Arg Cys
195 200 205
Asn Leu Gln Ser Leu Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala
210 215 220
Ala Met Pro His Asp Asp Asn Gln Asn
225 230
<210> 156
<211> 213
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLl，同源于AtPYR1 A160C
<400> 156
Met Pro Tyr Ala Ala Thr Arg Thr Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Ala Ser Asn Gly Arg Ala Val Ala Ala Cys Ala Gly His Ala Gly Val
20 25 30
Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala Ala Gly Gln
35 40 45
Cys Cys Ser Val Met Val Gln Ser Ile Ala Ala Pro Ala Asp Ala Val
50 55 60
Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Gly Tyr Lys Arg Phe
65 70 75 80
Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Val Glu Val Gly Ser
85 90 95
Val Arg Glu Leu Leu Val Val Ser Gly Leu Pro Ala Glu Asn Ser Arg
100 105 110
Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile Ser Phe Arg
115 120 125
Ile Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr
130 135 140
Val His Glu Ala Ala Ser Glu Gly Gly Pro Leu Thr Met Val Val Glu
145 150 155 160
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Val Glu Glu Thr Arg
165 170 175
Ile Phe Cys Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Glu Asp
180 185 190
Thr Val Ile Arg Gln Gln Ala Met Ala Ala Pro Ala Ala Pro His Asn
195 200 205
Asp His Asn His Ser
210
<210> 157
<211> 200
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLa，同源于AtPYR1 A160C
<400> 157
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Leu Ser Gly Gly Gly Ala Lys Ala Ala Ser His Gly Ala Ser Cys Ala
20 25 30
Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala Ala Arg Ala
35 40 45
Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala Pro Val Gly
50 55 60
Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys
65 70 75 80
His Phe Ile Arg Ser Cys Arg Leu Val Gly Gly Gly Asp Val Ala Val
85 90 95
Gly Ser Val Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Thr Ser
100 105 110
Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser
115 120 125
Phe Arg Val Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val
130 135 140
Thr Thr Val His Glu Ala Gly Ala Gly Ala Gly Thr Gly Thr Val Val
145 150 155 160
Val Glu Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala Asp Glu
165 170 175
Thr Arg Val Phe Cys Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
180 185 190
Ala Arg Thr Ala Glu Arg Leu Ala
195 200
<210> 158
<211> 220
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLb，同源于AtPYR1 A160C
<400> 158
Met Pro Cys Ile Gln Ala Ser Ser Pro Gly Gly Met Pro His Gln His
1 5 10 15
Gly Arg Gly Arg Val Leu Gly Gly Gly Val Gly Cys Ala Ala Glu Val
20 25 30
Ala Ala Ala Val Ala Ala Ser Ala Gly Gly Met Arg Cys Gly Ala His
35 40 45
Asp Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala
50 55 60
Ala Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val Ala Ala
65 70 75 80
Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Val Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly
100 105 110
Gly Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala
115 120 125
Ala Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val
130 135 140
Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Arg Asn Tyr Leu
145 150 155 160
Ser Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr
165 170 175
Val Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro
180 185 190
Glu Asp Thr Arg Val Phe Cys Asp Thr Ile Val Lys Cys Asn Leu Gln
195 200 205
Ser Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
210 215 220
<210> 159
<211> 218
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLo，同源于AtPYR1 A160C
<400> 159
Met Pro Cys Leu Gln Ala Ser Ser Pro Gly Ser Met Pro Tyr Gln His
1 5 10 15
His Gly Arg Gly Val Gly Cys Ala Ala Glu Ala Gly Ala Ala Val Gly
20 25 30
Ala Ser Ala Gly Thr Gly Thr Arg Cys Gly Ala His Asp Gly Glu Val
35 40 45
Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Pro Gly Pro Gly
50 55 60
Arg Cys Cys Ser Ala Val Val Gln Arg Val Ala Ala Pro Ala Glu Ala
65 70 75 80
Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Ala Tyr Lys Arg
85 90 95
Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly Val Gly Thr
100 105 110
Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Ala Ser Ser Arg
115 120 125
Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu Ser Phe Arg
130 135 140
Val Val Gly Gly Glu His Arg Leu Gln Asn Tyr Leu Ser Val Thr Thr
145 150 155 160
Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val Val Val Glu
165 170 175
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu Asp Thr Arg
180 185 190
Val Phe Cys Asp Thr Ile Val Lys Cys Asn Leu Gln Ser Leu Ala Thr
195 200 205
Thr Ala Glu Lys Leu Ala Leu Ala Ala Val
210 215
<210> 160
<211> 212
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLi，同源于AtPYR1 A160C
<400> 160
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Val Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Asp Pro Ala Ile Cys Thr Val Val Leu Glu Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Cys
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 161
<211> 205
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLk，同源于AtPYR1 A160C
<400> 161
Met Asp Gln Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Pro Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Val
65 70 75 80
Arg Pro Asp Pro Asp Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg
85 90 95
Glu Val Cys Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp His Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Gly Pro Gly Ile Cys Thr Val Val Leu Glu Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Cys
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Ser Thr Ser Ser Ser Ala Pro Pro Pro Pro Ser Glu
195 200 205
<210> 162
<211> 188
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLj，同源于AtPYR1 A160C
<400> 162
Met Glu Pro His Met Glu Ser Ala Leu Arg Gln Gly Leu Ser Glu Ala
1 5 10 15
Glu Gln Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro
20 25 30
Gly Arg Ala Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp
35 40 45
Ala Pro Leu Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro
50 55 60
Gln Arg Tyr Lys His Phe Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp
65 70 75 80
Gly Ala Thr Val Gly Ser Val Arg Glu Val Thr Val Val Ser Gly Leu
85 90 95
Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg
100 105 110
His Ile Leu Ser Phe Arg Val Val Gly Gly Asp His Arg Leu Arg Asn
115 120 125
Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys
130 135 140
Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu
145 150 155 160
Glu Asp Thr Arg Met Phe Cys Asp Thr Val Val Lys Leu Asn Leu Gln
165 170 175
Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn
180 185
<210> 163
<211> 214
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLd，同源于AtPYR1 A160C
<400> 163
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Ala Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Pro Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Phe Val Gly Gly Asp His Arg
130 135 140
Leu Gln Asn Tyr Ser Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly
145 150 155 160
Pro Tyr Cys Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly
165 170 175
Asn Thr Glu Glu Asp Thr Arg Met Phe Cys Asp Thr Val Val Lys Leu
180 185 190
Asn Leu Gln Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln
195 200 205
Thr Glu Pro Leu Asp Arg
210
<210> 164
<211> 209
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLf，同源于AtPYR1 A160C
<400> 164
Met Val Met Val Glu Met Asp Gly Gly Val Gly Gly Gly Gly Gly Gly
1 5 10 15
Gly Gln Thr Pro Ala Pro Arg Arg Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His
35 40 45
Glu Pro Arg Glu His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys
50 55 60
Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu Met Lys Gly Asn Ile
85 90 95
Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala
100 105 110
Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile
115 120 125
Leu Ser Val Arg Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser
130 135 140
Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys Val Val
145 150 155 160
Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu Glu Asp
165 170 175
Thr Arg Met Phe Cys Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu
180 185 190
Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln Thr Glu Pro Leu Asp
195 200 205
Arg
<210> 165
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLe，同源于AtPYR1 A160C
<400> 165
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His
20 25 30
Ala Pro Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Arg Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Cys Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Asn Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Leu Ile Asp Gln
195
<210> 166
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLg，同源于AtPYR1 A160C
<400> 166
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Thr Glu Tyr Val Arg Arg Leu His Arg His
20 25 30
Ala Pro Ala Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Ser Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Cys Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Lys Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Pro Ile Asp Gln
195
<210> 167
<211> 233
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLc，同源于AtPYR1 A160I
<400> 167
Met Arg Glu Arg Asn Ser Ser Ile Asp Gln Glu His Gln Arg Gly Ser
1 5 10 15
Ser Ser Arg Ser Thr Met Pro Phe Ala Ala Ser Arg Thr Ser Gln Gln
20 25 30
Gln His Ser Arg Val Ala Thr Asn Gly Arg Ala Val Ala Val Cys Ala
35 40 45
Gly His Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala
50 55 60
Val Ala Ala Gly Gln Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala
65 70 75 80
Pro Val Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Arg Tyr Lys Arg Phe Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly
100 105 110
Ala Glu Val Gly Ser Val Arg Glu Leu Leu Leu Val Ser Gly Leu Pro
115 120 125
Ala Glu Ser Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg
130 135 140
Val Ile Ser Phe Arg Val Leu Gly Gly Asp His Arg Leu Ala Asn Tyr
145 150 155 160
Arg Ser Val Thr Thr Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg
165 170 175
Pro Leu Thr Met Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Val Glu Glu Thr Arg Ile Phe Ile Asp Thr Ile Val Arg Cys
195 200 205
Asn Leu Gln Ser Leu Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala
210 215 220
Ala Met Pro His Asp Asp Asn Gln Asn
225 230
<210> 168
<211> 213
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLl，同源于AtPYR1 A160I
<400> 168
Met Pro Tyr Ala Ala Thr Arg Thr Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Ala Ser Asn Gly Arg Ala Val Ala Ala Cys Ala Gly His Ala Gly Val
20 25 30
Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala Ala Gly Gln
35 40 45
Cys Cys Ser Val Met Val Gln Ser Ile Ala Ala Pro Ala Asp Ala Val
50 55 60
Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Gly Tyr Lys Arg Phe
65 70 75 80
Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Val Glu Val Gly Ser
85 90 95
Val Arg Glu Leu Leu Val Val Ser Gly Leu Pro Ala Glu Asn Ser Arg
100 105 110
Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile Ser Phe Arg
115 120 125
Ile Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr
130 135 140
Val His Glu Ala Ala Ser Glu Gly Gly Pro Leu Thr Met Val Val Glu
145 150 155 160
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Val Glu Glu Thr Arg
165 170 175
Ile Phe Ile Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Glu Asp
180 185 190
Thr Val Ile Arg Gln Gln Ala Met Ala Ala Pro Ala Ala Pro His Asn
195 200 205
Asp His Asn His Ser
210
<210> 169
<211> 200
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLa，同源于AtPYR1 A160I
<400> 169
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Leu Ser Gly Gly Gly Ala Lys Ala Ala Ser His Gly Ala Ser Cys Ala
20 25 30
Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala Ala Arg Ala
35 40 45
Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala Pro Val Gly
50 55 60
Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys
65 70 75 80
His Phe Ile Arg Ser Cys Arg Leu Val Gly Gly Gly Asp Val Ala Val
85 90 95
Gly Ser Val Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Thr Ser
100 105 110
Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser
115 120 125
Phe Arg Val Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val
130 135 140
Thr Thr Val His Glu Ala Gly Ala Gly Ala Gly Thr Gly Thr Val Val
145 150 155 160
Val Glu Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala Asp Glu
165 170 175
Thr Arg Val Phe Ile Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
180 185 190
Ala Arg Thr Ala Glu Arg Leu Ala
195 200
<210> 170
<211> 220
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLb，同源于AtPYR1 A160I
<400> 170
Met Pro Cys Ile Gln Ala Ser Ser Pro Gly Gly Met Pro His Gln His
1 5 10 15
Gly Arg Gly Arg Val Leu Gly Gly Gly Val Gly Cys Ala Ala Glu Val
20 25 30
Ala Ala Ala Val Ala Ala Ser Ala Gly Gly Met Arg Cys Gly Ala His
35 40 45
Asp Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala
50 55 60
Ala Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val Ala Ala
65 70 75 80
Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Val Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly
100 105 110
Gly Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala
115 120 125
Ala Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val
130 135 140
Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Arg Asn Tyr Leu
145 150 155 160
Ser Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr
165 170 175
Val Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro
180 185 190
Glu Asp Thr Arg Val Phe Ile Asp Thr Ile Val Lys Cys Asn Leu Gln
195 200 205
Ser Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
210 215 220
<210> 171
<211> 218
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLo，同源于AtPYR1 A160I
<400> 171
Met Pro Cys Leu Gln Ala Ser Ser Pro Gly Ser Met Pro Tyr Gln His
1 5 10 15
His Gly Arg Gly Val Gly Cys Ala Ala Glu Ala Gly Ala Ala Val Gly
20 25 30
Ala Ser Ala Gly Thr Gly Thr Arg Cys Gly Ala His Asp Gly Glu Val
35 40 45
Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Pro Gly Pro Gly
50 55 60
Arg Cys Cys Ser Ala Val Val Gln Arg Val Ala Ala Pro Ala Glu Ala
65 70 75 80
Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Ala Tyr Lys Arg
85 90 95
Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly Val Gly Thr
100 105 110
Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Ala Ser Ser Arg
115 120 125
Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu Ser Phe Arg
130 135 140
Val Val Gly Gly Glu His Arg Leu Gln Asn Tyr Leu Ser Val Thr Thr
145 150 155 160
Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val Val Val Glu
165 170 175
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu Asp Thr Arg
180 185 190
Val Phe Ile Asp Thr Ile Val Lys Cys Asn Leu Gln Ser Leu Ala Thr
195 200 205
Thr Ala Glu Lys Leu Ala Leu Ala Ala Val
210 215
<210> 172
<211> 212
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLi，同源于AtPYR1 A160I
<400> 172
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Val Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Asp Pro Ala Ile Cys Thr Val Val Leu Glu Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ile
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 173
<211> 205
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLk，同源于AtPYR1 A160I
<400> 173
Met Asp Gln Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Pro Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Val
65 70 75 80
Arg Pro Asp Pro Asp Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg
85 90 95
Glu Val Cys Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp His Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Gly Pro Gly Ile Cys Thr Val Val Leu Glu Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ile
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Ser Thr Ser Ser Ser Ala Pro Pro Pro Pro Ser Glu
195 200 205
<210> 174
<211> 188
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLj，同源于AtPYR1 A160I
<400> 174
Met Glu Pro His Met Glu Ser Ala Leu Arg Gln Gly Leu Ser Glu Ala
1 5 10 15
Glu Gln Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro
20 25 30
Gly Arg Ala Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp
35 40 45
Ala Pro Leu Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro
50 55 60
Gln Arg Tyr Lys His Phe Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp
65 70 75 80
Gly Ala Thr Val Gly Ser Val Arg Glu Val Thr Val Val Ser Gly Leu
85 90 95
Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg
100 105 110
His Ile Leu Ser Phe Arg Val Val Gly Gly Asp His Arg Leu Arg Asn
115 120 125
Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys
130 135 140
Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu
145 150 155 160
Glu Asp Thr Arg Met Phe Ile Asp Thr Val Val Lys Leu Asn Leu Gln
165 170 175
Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn
180 185
<210> 175
<211> 214
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLd，同源于AtPYR1 A160I
<400> 175
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Ala Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Pro Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Phe Val Gly Gly Asp His Arg
130 135 140
Leu Gln Asn Tyr Ser Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly
145 150 155 160
Pro Tyr Cys Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly
165 170 175
Asn Thr Glu Glu Asp Thr Arg Met Phe Ile Asp Thr Val Val Lys Leu
180 185 190
Asn Leu Gln Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln
195 200 205
Thr Glu Pro Leu Asp Arg
210
<210> 176
<211> 209
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLf，同源于AtPYR1 A160I
<400> 176
Met Val Met Val Glu Met Asp Gly Gly Val Gly Gly Gly Gly Gly Gly
1 5 10 15
Gly Gln Thr Pro Ala Pro Arg Arg Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His
35 40 45
Glu Pro Arg Glu His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys
50 55 60
Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu Met Lys Gly Asn Ile
85 90 95
Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala
100 105 110
Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile
115 120 125
Leu Ser Val Arg Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser
130 135 140
Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys Val Val
145 150 155 160
Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu Glu Asp
165 170 175
Thr Arg Met Phe Ile Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu
180 185 190
Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln Thr Glu Pro Leu Asp
195 200 205
Arg
<210> 177
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLe，同源于AtPYR1 A160I
<400> 177
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His
20 25 30
Ala Pro Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Arg Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Ile Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Asn Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Leu Ile Asp Gln
195
<210> 178
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLg，同源于AtPYR1 A160I
<400> 178
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Thr Glu Tyr Val Arg Arg Leu His Arg His
20 25 30
Ala Pro Ala Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Ser Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Ile Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Lys Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Pro Ile Asp Gln
195
<210> 179
<211> 212
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLi，同源于AtPYR1 A160V
<400> 179
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Val Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Asp Pro Ala Ile Cys Thr Val Val Leu Glu Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 180
<211> 205
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLk，同源于AtPYR1 A160V
<400> 180
Met Asp Gln Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Pro Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Val
65 70 75 80
Arg Pro Asp Pro Asp Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg
85 90 95
Glu Val Cys Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp His Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Gly Pro Gly Ile Cys Thr Val Val Leu Glu Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Ser Thr Ser Ser Ser Ala Pro Pro Pro Pro Ser Glu
195 200 205
<210> 181
<211> 188
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLj，同源于AtPYR1 A160V
<400> 181
Met Glu Pro His Met Glu Ser Ala Leu Arg Gln Gly Leu Ser Glu Ala
1 5 10 15
Glu Gln Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro
20 25 30
Gly Arg Ala Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp
35 40 45
Ala Pro Leu Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro
50 55 60
Gln Arg Tyr Lys His Phe Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp
65 70 75 80
Gly Ala Thr Val Gly Ser Val Arg Glu Val Thr Val Val Ser Gly Leu
85 90 95
Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg
100 105 110
His Ile Leu Ser Phe Arg Val Val Gly Gly Asp His Arg Leu Arg Asn
115 120 125
Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys
130 135 140
Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu
145 150 155 160
Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln
165 170 175
Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn
180 185
<210> 182
<211> 233
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLc，同源于AtPYR1 E141C
<400> 182
Met Arg Glu Arg Asn Ser Ser Ile Asp Gln Glu His Gln Arg Gly Ser
1 5 10 15
Ser Ser Arg Ser Thr Met Pro Phe Ala Ala Ser Arg Thr Ser Gln Gln
20 25 30
Gln His Ser Arg Val Ala Thr Asn Gly Arg Ala Val Ala Val Cys Ala
35 40 45
Gly His Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala
50 55 60
Val Ala Ala Gly Gln Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala
65 70 75 80
Pro Val Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Arg Tyr Lys Arg Phe Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly
100 105 110
Ala Glu Val Gly Ser Val Arg Glu Leu Leu Leu Val Ser Gly Leu Pro
115 120 125
Ala Glu Ser Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg
130 135 140
Val Ile Ser Phe Arg Val Leu Gly Gly Asp His Arg Leu Ala Asn Tyr
145 150 155 160
Arg Ser Val Thr Thr Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg
165 170 175
Pro Leu Thr Met Val Val Cys Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Val Glu Glu Thr Arg Ile Phe Val Asp Thr Ile Val Arg Cys
195 200 205
Asn Leu Gln Ser Leu Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala
210 215 220
Ala Met Pro His Asp Asp Asn Gln Asn
225 230
<210> 183
<211> 213
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLl，同源于AtPYR1 E141C
<400> 183
Met Pro Tyr Ala Ala Thr Arg Thr Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Ala Ser Asn Gly Arg Ala Val Ala Ala Cys Ala Gly His Ala Gly Val
20 25 30
Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala Ala Gly Gln
35 40 45
Cys Cys Ser Val Met Val Gln Ser Ile Ala Ala Pro Ala Asp Ala Val
50 55 60
Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Gly Tyr Lys Arg Phe
65 70 75 80
Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Val Glu Val Gly Ser
85 90 95
Val Arg Glu Leu Leu Val Val Ser Gly Leu Pro Ala Glu Asn Ser Arg
100 105 110
Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile Ser Phe Arg
115 120 125
Ile Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr
130 135 140
Val His Glu Ala Ala Ser Glu Gly Gly Pro Leu Thr Met Val Val Cys
145 150 155 160
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Val Glu Glu Thr Arg
165 170 175
Ile Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Glu Asp
180 185 190
Thr Val Ile Arg Gln Gln Ala Met Ala Ala Pro Ala Ala Pro His Asn
195 200 205
Asp His Asn His Ser
210
<210> 184
<211> 200
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLa，同源于AtPYR1 E141C
<400> 184
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Leu Ser Gly Gly Gly Ala Lys Ala Ala Ser His Gly Ala Ser Cys Ala
20 25 30
Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala Ala Arg Ala
35 40 45
Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala Pro Val Gly
50 55 60
Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys
65 70 75 80
His Phe Ile Arg Ser Cys Arg Leu Val Gly Gly Gly Asp Val Ala Val
85 90 95
Gly Ser Val Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Thr Ser
100 105 110
Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser
115 120 125
Phe Arg Val Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val
130 135 140
Thr Thr Val His Glu Ala Gly Ala Gly Ala Gly Thr Gly Thr Val Val
145 150 155 160
Val Cys Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala Asp Glu
165 170 175
Thr Arg Val Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
180 185 190
Ala Arg Thr Ala Glu Arg Leu Ala
195 200
<210> 185
<211> 220
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLb，同源于AtPYR1 E141C
<400> 185
Met Pro Cys Ile Gln Ala Ser Ser Pro Gly Gly Met Pro His Gln His
1 5 10 15
Gly Arg Gly Arg Val Leu Gly Gly Gly Val Gly Cys Ala Ala Glu Val
20 25 30
Ala Ala Ala Val Ala Ala Ser Ala Gly Gly Met Arg Cys Gly Ala His
35 40 45
Asp Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala
50 55 60
Ala Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val Ala Ala
65 70 75 80
Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Val Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly
100 105 110
Gly Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala
115 120 125
Ala Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val
130 135 140
Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Arg Asn Tyr Leu
145 150 155 160
Ser Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr
165 170 175
Val Val Val Cys Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro
180 185 190
Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln
195 200 205
Ser Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
210 215 220
<210> 186
<211> 218
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLo，同源于AtPYR1 E141C
<400> 186
Met Pro Cys Leu Gln Ala Ser Ser Pro Gly Ser Met Pro Tyr Gln His
1 5 10 15
His Gly Arg Gly Val Gly Cys Ala Ala Glu Ala Gly Ala Ala Val Gly
20 25 30
Ala Ser Ala Gly Thr Gly Thr Arg Cys Gly Ala His Asp Gly Glu Val
35 40 45
Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Pro Gly Pro Gly
50 55 60
Arg Cys Cys Ser Ala Val Val Gln Arg Val Ala Ala Pro Ala Glu Ala
65 70 75 80
Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Ala Tyr Lys Arg
85 90 95
Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly Val Gly Thr
100 105 110
Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Ala Ser Ser Arg
115 120 125
Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu Ser Phe Arg
130 135 140
Val Val Gly Gly Glu His Arg Leu Gln Asn Tyr Leu Ser Val Thr Thr
145 150 155 160
Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val Val Val Cys
165 170 175
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu Asp Thr Arg
180 185 190
Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln Ser Leu Ala Thr
195 200 205
Thr Ala Glu Lys Leu Ala Leu Ala Ala Val
210 215
<210> 187
<211> 212
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLi，同源于AtPYR1 E141C
<400> 187
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Val Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Asp Pro Ala Ile Cys Thr Val Val Leu Cys Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 188
<211> 205
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLk，同源于AtPYR1 E141C
<400> 188
Met Asp Gln Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Pro Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Val
65 70 75 80
Arg Pro Asp Pro Asp Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg
85 90 95
Glu Val Cys Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp His Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Gly Pro Gly Ile Cys Thr Val Val Leu Cys Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Ser Thr Ser Ser Ser Ala Pro Pro Pro Pro Ser Glu
195 200 205
<210> 189
<211> 188
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLj，同源于AtPYR1 E141C
<400> 189
Met Glu Pro His Met Glu Ser Ala Leu Arg Gln Gly Leu Ser Glu Ala
1 5 10 15
Glu Gln Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro
20 25 30
Gly Arg Ala Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp
35 40 45
Ala Pro Leu Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro
50 55 60
Gln Arg Tyr Lys His Phe Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp
65 70 75 80
Gly Ala Thr Val Gly Ser Val Arg Glu Val Thr Val Val Ser Gly Leu
85 90 95
Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg
100 105 110
His Ile Leu Ser Phe Arg Val Val Gly Gly Asp His Arg Leu Arg Asn
115 120 125
Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys
130 135 140
Val Val Leu Cys Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu
145 150 155 160
Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln
165 170 175
Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn
180 185
<210> 190
<211> 214
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLd，同源于AtPYR1 E141C
<400> 190
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Ala Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Pro Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Phe Val Gly Gly Asp His Arg
130 135 140
Leu Gln Asn Tyr Ser Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly
145 150 155 160
Pro Tyr Cys Val Val Leu Cys Ser Tyr Val Val Asp Val Pro Asp Gly
165 170 175
Asn Thr Glu Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu
180 185 190
Asn Leu Gln Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln
195 200 205
Thr Glu Pro Leu Asp Arg
210
<210> 191
<211> 209
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLf，同源于AtPYR1 E141C
<400> 191
Met Val Met Val Glu Met Asp Gly Gly Val Gly Gly Gly Gly Gly Gly
1 5 10 15
Gly Gln Thr Pro Ala Pro Arg Arg Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His
35 40 45
Glu Pro Arg Glu His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys
50 55 60
Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu Met Lys Gly Asn Ile
85 90 95
Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala
100 105 110
Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile
115 120 125
Leu Ser Val Arg Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser
130 135 140
Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys Val Val
145 150 155 160
Leu Cys Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu Glu Asp
165 170 175
Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu
180 185 190
Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln Thr Glu Pro Leu Asp
195 200 205
Arg
<210> 192
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLe，同源于AtPYR1 E141C
<400> 192
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His
20 25 30
Ala Pro Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Arg Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Cys Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Asn Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Leu Ile Asp Gln
195
<210> 193
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLg，同源于AtPYR1 E141C
<400> 193
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Thr Glu Tyr Val Arg Arg Leu His Arg His
20 25 30
Ala Pro Ala Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Ser Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Cys Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Lys Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Pro Ile Asp Gln
195
<210> 194
<211> 233
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLc，同源于AtPYR1 E141I
<400> 194
Met Arg Glu Arg Asn Ser Ser Ile Asp Gln Glu His Gln Arg Gly Ser
1 5 10 15
Ser Ser Arg Ser Thr Met Pro Phe Ala Ala Ser Arg Thr Ser Gln Gln
20 25 30
Gln His Ser Arg Val Ala Thr Asn Gly Arg Ala Val Ala Val Cys Ala
35 40 45
Gly His Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala
50 55 60
Val Ala Ala Gly Gln Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala
65 70 75 80
Pro Val Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Arg Tyr Lys Arg Phe Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly
100 105 110
Ala Glu Val Gly Ser Val Arg Glu Leu Leu Leu Val Ser Gly Leu Pro
115 120 125
Ala Glu Ser Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg
130 135 140
Val Ile Ser Phe Arg Val Leu Gly Gly Asp His Arg Leu Ala Asn Tyr
145 150 155 160
Arg Ser Val Thr Thr Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg
165 170 175
Pro Leu Thr Met Val Val Ile Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Val Glu Glu Thr Arg Ile Phe Val Asp Thr Ile Val Arg Cys
195 200 205
Asn Leu Gln Ser Leu Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala
210 215 220
Ala Met Pro His Asp Asp Asn Gln Asn
225 230
<210> 195
<211> 213
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLl，同源于AtPYR1 E141I
<400> 195
Met Pro Tyr Ala Ala Thr Arg Thr Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Ala Ser Asn Gly Arg Ala Val Ala Ala Cys Ala Gly His Ala Gly Val
20 25 30
Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala Ala Gly Gln
35 40 45
Cys Cys Ser Val Met Val Gln Ser Ile Ala Ala Pro Ala Asp Ala Val
50 55 60
Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Gly Tyr Lys Arg Phe
65 70 75 80
Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Val Glu Val Gly Ser
85 90 95
Val Arg Glu Leu Leu Val Val Ser Gly Leu Pro Ala Glu Asn Ser Arg
100 105 110
Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile Ser Phe Arg
115 120 125
Ile Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr
130 135 140
Val His Glu Ala Ala Ser Glu Gly Gly Pro Leu Thr Met Val Val Ile
145 150 155 160
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Val Glu Glu Thr Arg
165 170 175
Ile Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Glu Asp
180 185 190
Thr Val Ile Arg Gln Gln Ala Met Ala Ala Pro Ala Ala Pro His Asn
195 200 205
Asp His Asn His Ser
210
<210> 196
<211> 200
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLa，同源于AtPYR1 E141I
<400> 196
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Leu Ser Gly Gly Gly Ala Lys Ala Ala Ser His Gly Ala Ser Cys Ala
20 25 30
Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala Ala Arg Ala
35 40 45
Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala Pro Val Gly
50 55 60
Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys
65 70 75 80
His Phe Ile Arg Ser Cys Arg Leu Val Gly Gly Gly Asp Val Ala Val
85 90 95
Gly Ser Val Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Thr Ser
100 105 110
Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser
115 120 125
Phe Arg Val Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val
130 135 140
Thr Thr Val His Glu Ala Gly Ala Gly Ala Gly Thr Gly Thr Val Val
145 150 155 160
Val Ile Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala Asp Glu
165 170 175
Thr Arg Val Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
180 185 190
Ala Arg Thr Ala Glu Arg Leu Ala
195 200
<210> 197
<211> 220
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLb，同源于AtPYR1 E141I
<400> 197
Met Pro Cys Ile Gln Ala Ser Ser Pro Gly Gly Met Pro His Gln His
1 5 10 15
Gly Arg Gly Arg Val Leu Gly Gly Gly Val Gly Cys Ala Ala Glu Val
20 25 30
Ala Ala Ala Val Ala Ala Ser Ala Gly Gly Met Arg Cys Gly Ala His
35 40 45
Asp Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala
50 55 60
Ala Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val Ala Ala
65 70 75 80
Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Val Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly
100 105 110
Gly Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala
115 120 125
Ala Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val
130 135 140
Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Arg Asn Tyr Leu
145 150 155 160
Ser Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr
165 170 175
Val Val Val Ile Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro
180 185 190
Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln
195 200 205
Ser Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
210 215 220
<210> 198
<211> 218
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLo，同源于AtPYR1 E141I
<400> 198
Met Pro Cys Leu Gln Ala Ser Ser Pro Gly Ser Met Pro Tyr Gln His
1 5 10 15
His Gly Arg Gly Val Gly Cys Ala Ala Glu Ala Gly Ala Ala Val Gly
20 25 30
Ala Ser Ala Gly Thr Gly Thr Arg Cys Gly Ala His Asp Gly Glu Val
35 40 45
Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Pro Gly Pro Gly
50 55 60
Arg Cys Cys Ser Ala Val Val Gln Arg Val Ala Ala Pro Ala Glu Ala
65 70 75 80
Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Ala Tyr Lys Arg
85 90 95
Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly Val Gly Thr
100 105 110
Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Ala Ser Ser Arg
115 120 125
Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu Ser Phe Arg
130 135 140
Val Val Gly Gly Glu His Arg Leu Gln Asn Tyr Leu Ser Val Thr Thr
145 150 155 160
Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val Val Val Ile
165 170 175
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu Asp Thr Arg
180 185 190
Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln Ser Leu Ala Thr
195 200 205
Thr Ala Glu Lys Leu Ala Leu Ala Ala Val
210 215
<210> 199
<211> 212
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLi，同源于AtPYR1 E141I
<400> 199
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Val Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Asp Pro Ala Ile Cys Thr Val Val Leu Ile Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 200
<211> 205
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLk，同源于AtPYR1 E141I
<400> 200
Met Asp Gln Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Pro Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Val
65 70 75 80
Arg Pro Asp Pro Asp Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg
85 90 95
Glu Val Cys Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp His Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Gly Pro Gly Ile Cys Thr Val Val Leu Ile Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Ser Thr Ser Ser Ser Ala Pro Pro Pro Pro Ser Glu
195 200 205
<210> 201
<211> 188
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLj，同源于AtPYR1 E141I
<400> 201
Met Glu Pro His Met Glu Ser Ala Leu Arg Gln Gly Leu Ser Glu Ala
1 5 10 15
Glu Gln Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro
20 25 30
Gly Arg Ala Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp
35 40 45
Ala Pro Leu Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro
50 55 60
Gln Arg Tyr Lys His Phe Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp
65 70 75 80
Gly Ala Thr Val Gly Ser Val Arg Glu Val Thr Val Val Ser Gly Leu
85 90 95
Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg
100 105 110
His Ile Leu Ser Phe Arg Val Val Gly Gly Asp His Arg Leu Arg Asn
115 120 125
Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys
130 135 140
Val Val Leu Ile Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu
145 150 155 160
Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln
165 170 175
Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn
180 185
<210> 202
<211> 214
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLd，同源于AtPYR1 E141I
<400> 202
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Ala Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Pro Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Phe Val Gly Gly Asp His Arg
130 135 140
Leu Gln Asn Tyr Ser Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly
145 150 155 160
Pro Tyr Cys Val Val Leu Ile Ser Tyr Val Val Asp Val Pro Asp Gly
165 170 175
Asn Thr Glu Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu
180 185 190
Asn Leu Gln Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln
195 200 205
Thr Glu Pro Leu Asp Arg
210
<210> 203
<211> 209
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLf，同源于AtPYR1 E141I
<400> 203
Met Val Met Val Glu Met Asp Gly Gly Val Gly Gly Gly Gly Gly Gly
1 5 10 15
Gly Gln Thr Pro Ala Pro Arg Arg Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His
35 40 45
Glu Pro Arg Glu His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys
50 55 60
Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu Met Lys Gly Asn Ile
85 90 95
Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala
100 105 110
Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile
115 120 125
Leu Ser Val Arg Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser
130 135 140
Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys Val Val
145 150 155 160
Leu Ile Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu Glu Asp
165 170 175
Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu
180 185 190
Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln Thr Glu Pro Leu Asp
195 200 205
Arg
<210> 204
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLe，同源于AtPYR1 E141I
<400> 204
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His
20 25 30
Ala Pro Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Arg Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Ile Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Asn Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Leu Ile Asp Gln
195
<210> 205
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLg，同源于AtPYR1 E141I
<400> 205
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Thr Glu Tyr Val Arg Arg Leu His Arg His
20 25 30
Ala Pro Ala Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Ser Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Ile Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Lys Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Pro Ile Asp Gln
195
<210> 206
<211> 233
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLc，同源于AtPYR1 E141L
<400> 206
Met Arg Glu Arg Asn Ser Ser Ile Asp Gln Glu His Gln Arg Gly Ser
1 5 10 15
Ser Ser Arg Ser Thr Met Pro Phe Ala Ala Ser Arg Thr Ser Gln Gln
20 25 30
Gln His Ser Arg Val Ala Thr Asn Gly Arg Ala Val Ala Val Cys Ala
35 40 45
Gly His Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala
50 55 60
Val Ala Ala Gly Gln Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala
65 70 75 80
Pro Val Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Arg Tyr Lys Arg Phe Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly
100 105 110
Ala Glu Val Gly Ser Val Arg Glu Leu Leu Leu Val Ser Gly Leu Pro
115 120 125
Ala Glu Ser Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg
130 135 140
Val Ile Ser Phe Arg Val Leu Gly Gly Asp His Arg Leu Ala Asn Tyr
145 150 155 160
Arg Ser Val Thr Thr Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg
165 170 175
Pro Leu Thr Met Val Val Leu Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Val Glu Glu Thr Arg Ile Phe Val Asp Thr Ile Val Arg Cys
195 200 205
Asn Leu Gln Ser Leu Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala
210 215 220
Ala Met Pro His Asp Asp Asn Gln Asn
225 230
<210> 207
<211> 213
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLl，同源于AtPYR1 E141L
<400> 207
Met Pro Tyr Ala Ala Thr Arg Thr Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Ala Ser Asn Gly Arg Ala Val Ala Ala Cys Ala Gly His Ala Gly Val
20 25 30
Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala Ala Gly Gln
35 40 45
Cys Cys Ser Val Met Val Gln Ser Ile Ala Ala Pro Ala Asp Ala Val
50 55 60
Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Gly Tyr Lys Arg Phe
65 70 75 80
Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Val Glu Val Gly Ser
85 90 95
Val Arg Glu Leu Leu Val Val Ser Gly Leu Pro Ala Glu Asn Ser Arg
100 105 110
Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile Ser Phe Arg
115 120 125
Ile Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr
130 135 140
Val His Glu Ala Ala Ser Glu Gly Gly Pro Leu Thr Met Val Val Leu
145 150 155 160
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Val Glu Glu Thr Arg
165 170 175
Ile Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Glu Asp
180 185 190
Thr Val Ile Arg Gln Gln Ala Met Ala Ala Pro Ala Ala Pro His Asn
195 200 205
Asp His Asn His Ser
210
<210> 208
<211> 200
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLa，同源于AtPYR1 E141L
<400> 208
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Leu Ser Gly Gly Gly Ala Lys Ala Ala Ser His Gly Ala Ser Cys Ala
20 25 30
Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala Ala Arg Ala
35 40 45
Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala Pro Val Gly
50 55 60
Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys
65 70 75 80
His Phe Ile Arg Ser Cys Arg Leu Val Gly Gly Gly Asp Val Ala Val
85 90 95
Gly Ser Val Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Thr Ser
100 105 110
Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser
115 120 125
Phe Arg Val Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val
130 135 140
Thr Thr Val His Glu Ala Gly Ala Gly Ala Gly Thr Gly Thr Val Val
145 150 155 160
Val Leu Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala Asp Glu
165 170 175
Thr Arg Val Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
180 185 190
Ala Arg Thr Ala Glu Arg Leu Ala
195 200
<210> 209
<211> 220
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLb，同源于AtPYR1 E141L
<400> 209
Met Pro Cys Ile Gln Ala Ser Ser Pro Gly Gly Met Pro His Gln His
1 5 10 15
Gly Arg Gly Arg Val Leu Gly Gly Gly Val Gly Cys Ala Ala Glu Val
20 25 30
Ala Ala Ala Val Ala Ala Ser Ala Gly Gly Met Arg Cys Gly Ala His
35 40 45
Asp Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala
50 55 60
Ala Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val Ala Ala
65 70 75 80
Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Val Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly
100 105 110
Gly Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala
115 120 125
Ala Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val
130 135 140
Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Arg Asn Tyr Leu
145 150 155 160
Ser Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr
165 170 175
Val Val Val Leu Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro
180 185 190
Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln
195 200 205
Ser Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
210 215 220
<210> 210
<211> 218
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLo，同源于AtPYR1 E141L
<400> 210
Met Pro Cys Leu Gln Ala Ser Ser Pro Gly Ser Met Pro Tyr Gln His
1 5 10 15
His Gly Arg Gly Val Gly Cys Ala Ala Glu Ala Gly Ala Ala Val Gly
20 25 30
Ala Ser Ala Gly Thr Gly Thr Arg Cys Gly Ala His Asp Gly Glu Val
35 40 45
Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Pro Gly Pro Gly
50 55 60
Arg Cys Cys Ser Ala Val Val Gln Arg Val Ala Ala Pro Ala Glu Ala
65 70 75 80
Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Ala Tyr Lys Arg
85 90 95
Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly Val Gly Thr
100 105 110
Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Ala Ser Ser Arg
115 120 125
Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu Ser Phe Arg
130 135 140
Val Val Gly Gly Glu His Arg Leu Gln Asn Tyr Leu Ser Val Thr Thr
145 150 155 160
Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val Val Val Leu
165 170 175
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu Asp Thr Arg
180 185 190
Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln Ser Leu Ala Thr
195 200 205
Thr Ala Glu Lys Leu Ala Leu Ala Ala Val
210 215
<210> 211
<211> 212
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLi，同源于AtPYR1 E141L
<400> 211
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Val Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Asp Pro Ala Ile Cys Thr Val Val Leu Leu Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 212
<211> 205
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLk，同源于AtPYR1 E141L
<400> 212
Met Asp Gln Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Pro Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Val
65 70 75 80
Arg Pro Asp Pro Asp Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg
85 90 95
Glu Val Cys Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp His Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Gly Pro Gly Ile Cys Thr Val Val Leu Leu Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Ser Thr Ser Ser Ser Ala Pro Pro Pro Pro Ser Glu
195 200 205
<210> 213
<211> 188
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLj，同源于AtPYR1 E141L
<400> 213
Met Glu Pro His Met Glu Ser Ala Leu Arg Gln Gly Leu Ser Glu Ala
1 5 10 15
Glu Gln Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro
20 25 30
Gly Arg Ala Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp
35 40 45
Ala Pro Leu Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro
50 55 60
Gln Arg Tyr Lys His Phe Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp
65 70 75 80
Gly Ala Thr Val Gly Ser Val Arg Glu Val Thr Val Val Ser Gly Leu
85 90 95
Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg
100 105 110
His Ile Leu Ser Phe Arg Val Val Gly Gly Asp His Arg Leu Arg Asn
115 120 125
Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys
130 135 140
Val Val Leu Leu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu
145 150 155 160
Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln
165 170 175
Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn
180 185
<210> 214
<211> 214
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLd，同源于AtPYR1 E141L
<400> 214
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Ala Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Pro Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Phe Val Gly Gly Asp His Arg
130 135 140
Leu Gln Asn Tyr Ser Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly
145 150 155 160
Pro Tyr Cys Val Val Leu Leu Ser Tyr Val Val Asp Val Pro Asp Gly
165 170 175
Asn Thr Glu Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu
180 185 190
Asn Leu Gln Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln
195 200 205
Thr Glu Pro Leu Asp Arg
210
<210> 215
<211> 209
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLf，同源于AtPYR1 E141L
<400> 215
Met Val Met Val Glu Met Asp Gly Gly Val Gly Gly Gly Gly Gly Gly
1 5 10 15
Gly Gln Thr Pro Ala Pro Arg Arg Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His
35 40 45
Glu Pro Arg Glu His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys
50 55 60
Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu Met Lys Gly Asn Ile
85 90 95
Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala
100 105 110
Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile
115 120 125
Leu Ser Val Arg Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser
130 135 140
Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys Val Val
145 150 155 160
Leu Leu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu Glu Asp
165 170 175
Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu
180 185 190
Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln Thr Glu Pro Leu Asp
195 200 205
Arg
<210> 216
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLe，同源于AtPYR1 E141L
<400> 216
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His
20 25 30
Ala Pro Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Arg Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Leu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Asn Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Leu Ile Asp Gln
195
<210> 217
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLg，同源于AtPYR1 E141L
<400> 217
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Thr Glu Tyr Val Arg Arg Leu His Arg His
20 25 30
Ala Pro Ala Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Ser Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Leu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Lys Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Pro Ile Asp Gln
195
<210> 218
<211> 233
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLc，同源于AtPYR1 E141M
<400> 218
Met Arg Glu Arg Asn Ser Ser Ile Asp Gln Glu His Gln Arg Gly Ser
1 5 10 15
Ser Ser Arg Ser Thr Met Pro Phe Ala Ala Ser Arg Thr Ser Gln Gln
20 25 30
Gln His Ser Arg Val Ala Thr Asn Gly Arg Ala Val Ala Val Cys Ala
35 40 45
Gly His Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala
50 55 60
Val Ala Ala Gly Gln Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala
65 70 75 80
Pro Val Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Arg Tyr Lys Arg Phe Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly
100 105 110
Ala Glu Val Gly Ser Val Arg Glu Leu Leu Leu Val Ser Gly Leu Pro
115 120 125
Ala Glu Ser Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg
130 135 140
Val Ile Ser Phe Arg Val Leu Gly Gly Asp His Arg Leu Ala Asn Tyr
145 150 155 160
Arg Ser Val Thr Thr Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg
165 170 175
Pro Leu Thr Met Val Val Met Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Val Glu Glu Thr Arg Ile Phe Val Asp Thr Ile Val Arg Cys
195 200 205
Asn Leu Gln Ser Leu Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala
210 215 220
Ala Met Pro His Asp Asp Asn Gln Asn
225 230
<210> 219
<211> 213
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLl，同源于AtPYR1 E141M
<400> 219
Met Pro Tyr Ala Ala Thr Arg Thr Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Ala Ser Asn Gly Arg Ala Val Ala Ala Cys Ala Gly His Ala Gly Val
20 25 30
Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala Ala Gly Gln
35 40 45
Cys Cys Ser Val Met Val Gln Ser Ile Ala Ala Pro Ala Asp Ala Val
50 55 60
Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Gly Tyr Lys Arg Phe
65 70 75 80
Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Val Glu Val Gly Ser
85 90 95
Val Arg Glu Leu Leu Val Val Ser Gly Leu Pro Ala Glu Asn Ser Arg
100 105 110
Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile Ser Phe Arg
115 120 125
Ile Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr
130 135 140
Val His Glu Ala Ala Ser Glu Gly Gly Pro Leu Thr Met Val Val Met
145 150 155 160
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Val Glu Glu Thr Arg
165 170 175
Ile Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Glu Asp
180 185 190
Thr Val Ile Arg Gln Gln Ala Met Ala Ala Pro Ala Ala Pro His Asn
195 200 205
Asp His Asn His Ser
210
<210> 220
<211> 200
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLa，同源于AtPYR1 E141M
<400> 220
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Leu Ser Gly Gly Gly Ala Lys Ala Ala Ser His Gly Ala Ser Cys Ala
20 25 30
Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala Ala Arg Ala
35 40 45
Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala Pro Val Gly
50 55 60
Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys
65 70 75 80
His Phe Ile Arg Ser Cys Arg Leu Val Gly Gly Gly Asp Val Ala Val
85 90 95
Gly Ser Val Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Thr Ser
100 105 110
Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser
115 120 125
Phe Arg Val Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val
130 135 140
Thr Thr Val His Glu Ala Gly Ala Gly Ala Gly Thr Gly Thr Val Val
145 150 155 160
Val Met Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala Asp Glu
165 170 175
Thr Arg Val Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
180 185 190
Ala Arg Thr Ala Glu Arg Leu Ala
195 200
<210> 221
<211> 220
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLb，同源于AtPYR1 E141M
<400> 221
Met Pro Cys Ile Gln Ala Ser Ser Pro Gly Gly Met Pro His Gln His
1 5 10 15
Gly Arg Gly Arg Val Leu Gly Gly Gly Val Gly Cys Ala Ala Glu Val
20 25 30
Ala Ala Ala Val Ala Ala Ser Ala Gly Gly Met Arg Cys Gly Ala His
35 40 45
Asp Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala
50 55 60
Ala Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val Ala Ala
65 70 75 80
Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Val Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly
100 105 110
Gly Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala
115 120 125
Ala Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val
130 135 140
Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Arg Asn Tyr Leu
145 150 155 160
Ser Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr
165 170 175
Val Val Val Met Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro
180 185 190
Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln
195 200 205
Ser Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
210 215 220
<210> 222
<211> 218
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLo，同源于AtPYR1 E141M
<400> 222
Met Pro Cys Leu Gln Ala Ser Ser Pro Gly Ser Met Pro Tyr Gln His
1 5 10 15
His Gly Arg Gly Val Gly Cys Ala Ala Glu Ala Gly Ala Ala Val Gly
20 25 30
Ala Ser Ala Gly Thr Gly Thr Arg Cys Gly Ala His Asp Gly Glu Val
35 40 45
Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Pro Gly Pro Gly
50 55 60
Arg Cys Cys Ser Ala Val Val Gln Arg Val Ala Ala Pro Ala Glu Ala
65 70 75 80
Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Ala Tyr Lys Arg
85 90 95
Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly Val Gly Thr
100 105 110
Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Ala Ser Ser Arg
115 120 125
Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu Ser Phe Arg
130 135 140
Val Val Gly Gly Glu His Arg Leu Gln Asn Tyr Leu Ser Val Thr Thr
145 150 155 160
Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val Val Val Met
165 170 175
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu Asp Thr Arg
180 185 190
Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln Ser Leu Ala Thr
195 200 205
Thr Ala Glu Lys Leu Ala Leu Ala Ala Val
210 215
<210> 223
<211> 212
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLi，同源于AtPYR1 E141M
<400> 223
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Val Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Asp Pro Ala Ile Cys Thr Val Val Leu Met Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 224
<211> 205
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLk，同源于AtPYR1 E141M
<400> 224
Met Asp Gln Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Pro Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Val
65 70 75 80
Arg Pro Asp Pro Asp Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg
85 90 95
Glu Val Cys Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp His Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Gly Pro Gly Ile Cys Thr Val Val Leu Met Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Ser Thr Ser Ser Ser Ala Pro Pro Pro Pro Ser Glu
195 200 205
<210> 225
<211> 188
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLj，同源于AtPYR1 E141M
<400> 225
Met Glu Pro His Met Glu Ser Ala Leu Arg Gln Gly Leu Ser Glu Ala
1 5 10 15
Glu Gln Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro
20 25 30
Gly Arg Ala Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp
35 40 45
Ala Pro Leu Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro
50 55 60
Gln Arg Tyr Lys His Phe Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp
65 70 75 80
Gly Ala Thr Val Gly Ser Val Arg Glu Val Thr Val Val Ser Gly Leu
85 90 95
Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg
100 105 110
His Ile Leu Ser Phe Arg Val Val Gly Gly Asp His Arg Leu Arg Asn
115 120 125
Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys
130 135 140
Val Val Leu Met Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu
145 150 155 160
Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln
165 170 175
Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn
180 185
<210> 226
<211> 214
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLd，同源于AtPYR1 E141M
<400> 226
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Ala Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Pro Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Phe Val Gly Gly Asp His Arg
130 135 140
Leu Gln Asn Tyr Ser Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly
145 150 155 160
Pro Tyr Cys Val Val Leu Met Ser Tyr Val Val Asp Val Pro Asp Gly
165 170 175
Asn Thr Glu Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu
180 185 190
Asn Leu Gln Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln
195 200 205
Thr Glu Pro Leu Asp Arg
210
<210> 227
<211> 209
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLf，同源于AtPYR1 E141M
<400> 227
Met Val Met Val Glu Met Asp Gly Gly Val Gly Gly Gly Gly Gly Gly
1 5 10 15
Gly Gln Thr Pro Ala Pro Arg Arg Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His
35 40 45
Glu Pro Arg Glu His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys
50 55 60
Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu Met Lys Gly Asn Ile
85 90 95
Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala
100 105 110
Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile
115 120 125
Leu Ser Val Arg Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser
130 135 140
Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys Val Val
145 150 155 160
Leu Met Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu Glu Asp
165 170 175
Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu
180 185 190
Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln Thr Glu Pro Leu Asp
195 200 205
Arg
<210> 228
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLe，同源于AtPYR1 E141M
<400> 228
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His
20 25 30
Ala Pro Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Arg Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Met Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Asn Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Leu Ile Asp Gln
195
<210> 229
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLg，同源于AtPYR1 E141M
<400> 229
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Thr Glu Tyr Val Arg Arg Leu His Arg His
20 25 30
Ala Pro Ala Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Ser Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Met Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Lys Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Pro Ile Asp Gln
195
<210> 230
<211> 233
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLc，同源于AtPYR1 E141N
<400> 230
Met Arg Glu Arg Asn Ser Ser Ile Asp Gln Glu His Gln Arg Gly Ser
1 5 10 15
Ser Ser Arg Ser Thr Met Pro Phe Ala Ala Ser Arg Thr Ser Gln Gln
20 25 30
Gln His Ser Arg Val Ala Thr Asn Gly Arg Ala Val Ala Val Cys Ala
35 40 45
Gly His Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala
50 55 60
Val Ala Ala Gly Gln Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala
65 70 75 80
Pro Val Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Arg Tyr Lys Arg Phe Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly
100 105 110
Ala Glu Val Gly Ser Val Arg Glu Leu Leu Leu Val Ser Gly Leu Pro
115 120 125
Ala Glu Ser Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg
130 135 140
Val Ile Ser Phe Arg Val Leu Gly Gly Asp His Arg Leu Ala Asn Tyr
145 150 155 160
Arg Ser Val Thr Thr Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg
165 170 175
Pro Leu Thr Met Val Val Asn Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Val Glu Glu Thr Arg Ile Phe Val Asp Thr Ile Val Arg Cys
195 200 205
Asn Leu Gln Ser Leu Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala
210 215 220
Ala Met Pro His Asp Asp Asn Gln Asn
225 230
<210> 231
<211> 213
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLl，同源于AtPYR1 E141N
<400> 231
Met Pro Tyr Ala Ala Thr Arg Thr Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Ala Ser Asn Gly Arg Ala Val Ala Ala Cys Ala Gly His Ala Gly Val
20 25 30
Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala Ala Gly Gln
35 40 45
Cys Cys Ser Val Met Val Gln Ser Ile Ala Ala Pro Ala Asp Ala Val
50 55 60
Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Gly Tyr Lys Arg Phe
65 70 75 80
Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Val Glu Val Gly Ser
85 90 95
Val Arg Glu Leu Leu Val Val Ser Gly Leu Pro Ala Glu Asn Ser Arg
100 105 110
Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile Ser Phe Arg
115 120 125
Ile Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr
130 135 140
Val His Glu Ala Ala Ser Glu Gly Gly Pro Leu Thr Met Val Val Asn
145 150 155 160
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Val Glu Glu Thr Arg
165 170 175
Ile Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Glu Asp
180 185 190
Thr Val Ile Arg Gln Gln Ala Met Ala Ala Pro Ala Ala Pro His Asn
195 200 205
Asp His Asn His Ser
210
<210> 232
<211> 200
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLa，同源于AtPYR1 E141N
<400> 232
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Leu Ser Gly Gly Gly Ala Lys Ala Ala Ser His Gly Ala Ser Cys Ala
20 25 30
Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala Ala Arg Ala
35 40 45
Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala Pro Val Gly
50 55 60
Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys
65 70 75 80
His Phe Ile Arg Ser Cys Arg Leu Val Gly Gly Gly Asp Val Ala Val
85 90 95
Gly Ser Val Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Thr Ser
100 105 110
Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser
115 120 125
Phe Arg Val Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val
130 135 140
Thr Thr Val His Glu Ala Gly Ala Gly Ala Gly Thr Gly Thr Val Val
145 150 155 160
Val Asn Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala Asp Glu
165 170 175
Thr Arg Val Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
180 185 190
Ala Arg Thr Ala Glu Arg Leu Ala
195 200
<210> 233
<211> 220
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLb，同源于AtPYR1 E141N
<400> 233
Met Pro Cys Ile Gln Ala Ser Ser Pro Gly Gly Met Pro His Gln His
1 5 10 15
Gly Arg Gly Arg Val Leu Gly Gly Gly Val Gly Cys Ala Ala Glu Val
20 25 30
Ala Ala Ala Val Ala Ala Ser Ala Gly Gly Met Arg Cys Gly Ala His
35 40 45
Asp Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala
50 55 60
Ala Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val Ala Ala
65 70 75 80
Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Val Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly
100 105 110
Gly Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala
115 120 125
Ala Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val
130 135 140
Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Arg Asn Tyr Leu
145 150 155 160
Ser Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr
165 170 175
Val Val Val Asn Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro
180 185 190
Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln
195 200 205
Ser Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
210 215 220
<210> 234
<211> 218
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLo，同源于AtPYR1 E141N
<400> 234
Met Pro Cys Leu Gln Ala Ser Ser Pro Gly Ser Met Pro Tyr Gln His
1 5 10 15
His Gly Arg Gly Val Gly Cys Ala Ala Glu Ala Gly Ala Ala Val Gly
20 25 30
Ala Ser Ala Gly Thr Gly Thr Arg Cys Gly Ala His Asp Gly Glu Val
35 40 45
Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Pro Gly Pro Gly
50 55 60
Arg Cys Cys Ser Ala Val Val Gln Arg Val Ala Ala Pro Ala Glu Ala
65 70 75 80
Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Ala Tyr Lys Arg
85 90 95
Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly Val Gly Thr
100 105 110
Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Ala Ser Ser Arg
115 120 125
Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu Ser Phe Arg
130 135 140
Val Val Gly Gly Glu His Arg Leu Gln Asn Tyr Leu Ser Val Thr Thr
145 150 155 160
Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val Val Val Asn
165 170 175
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu Asp Thr Arg
180 185 190
Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln Ser Leu Ala Thr
195 200 205
Thr Ala Glu Lys Leu Ala Leu Ala Ala Val
210 215
<210> 235
<211> 212
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLi，同源于AtPYR1 E141N
<400> 235
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Val Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Asp Pro Ala Ile Cys Thr Val Val Leu Asn Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 236
<211> 205
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLk，同源于AtPYR1 E141N
<400> 236
Met Asp Gln Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Pro Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Val
65 70 75 80
Arg Pro Asp Pro Asp Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg
85 90 95
Glu Val Cys Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp His Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Gly Pro Gly Ile Cys Thr Val Val Leu Asn Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Ser Thr Ser Ser Ser Ala Pro Pro Pro Pro Ser Glu
195 200 205
<210> 237
<211> 188
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLj，同源于AtPYR1 E141N
<400> 237
Met Glu Pro His Met Glu Ser Ala Leu Arg Gln Gly Leu Ser Glu Ala
1 5 10 15
Glu Gln Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro
20 25 30
Gly Arg Ala Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp
35 40 45
Ala Pro Leu Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro
50 55 60
Gln Arg Tyr Lys His Phe Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp
65 70 75 80
Gly Ala Thr Val Gly Ser Val Arg Glu Val Thr Val Val Ser Gly Leu
85 90 95
Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg
100 105 110
His Ile Leu Ser Phe Arg Val Val Gly Gly Asp His Arg Leu Arg Asn
115 120 125
Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys
130 135 140
Val Val Leu Asn Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu
145 150 155 160
Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln
165 170 175
Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn
180 185
<210> 238
<211> 214
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLd，同源于AtPYR1 E141N
<400> 238
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Ala Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Pro Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Phe Val Gly Gly Asp His Arg
130 135 140
Leu Gln Asn Tyr Ser Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly
145 150 155 160
Pro Tyr Cys Val Val Leu Asn Ser Tyr Val Val Asp Val Pro Asp Gly
165 170 175
Asn Thr Glu Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu
180 185 190
Asn Leu Gln Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln
195 200 205
Thr Glu Pro Leu Asp Arg
210
<210> 239
<211> 209
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLf，同源于AtPYR1 E141N
<400> 239
Met Val Met Val Glu Met Asp Gly Gly Val Gly Gly Gly Gly Gly Gly
1 5 10 15
Gly Gln Thr Pro Ala Pro Arg Arg Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His
35 40 45
Glu Pro Arg Glu His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys
50 55 60
Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu Met Lys Gly Asn Ile
85 90 95
Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala
100 105 110
Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile
115 120 125
Leu Ser Val Arg Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser
130 135 140
Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys Val Val
145 150 155 160
Leu Asn Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu Glu Asp
165 170 175
Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu
180 185 190
Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln Thr Glu Pro Leu Asp
195 200 205
Arg
<210> 240
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLe，同源于AtPYR1 E141N
<400> 240
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His
20 25 30
Ala Pro Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Arg Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Asn Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Asn Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Leu Ile Asp Gln
195
<210> 241
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLg，同源于AtPYR1 E141N
<400> 241
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Thr Glu Tyr Val Arg Arg Leu His Arg His
20 25 30
Ala Pro Ala Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Ser Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Asn Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Lys Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Pro Ile Asp Gln
195
<210> 242
<211> 233
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLc，同源于AtPYR1 E141T
<400> 242
Met Arg Glu Arg Asn Ser Ser Ile Asp Gln Glu His Gln Arg Gly Ser
1 5 10 15
Ser Ser Arg Ser Thr Met Pro Phe Ala Ala Ser Arg Thr Ser Gln Gln
20 25 30
Gln His Ser Arg Val Ala Thr Asn Gly Arg Ala Val Ala Val Cys Ala
35 40 45
Gly His Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala
50 55 60
Val Ala Ala Gly Gln Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala
65 70 75 80
Pro Val Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Arg Tyr Lys Arg Phe Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly
100 105 110
Ala Glu Val Gly Ser Val Arg Glu Leu Leu Leu Val Ser Gly Leu Pro
115 120 125
Ala Glu Ser Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg
130 135 140
Val Ile Ser Phe Arg Val Leu Gly Gly Asp His Arg Leu Ala Asn Tyr
145 150 155 160
Arg Ser Val Thr Thr Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg
165 170 175
Pro Leu Thr Met Val Val Thr Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Val Glu Glu Thr Arg Ile Phe Val Asp Thr Ile Val Arg Cys
195 200 205
Asn Leu Gln Ser Leu Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala
210 215 220
Ala Met Pro His Asp Asp Asn Gln Asn
225 230
<210> 243
<211> 213
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLl，同源于AtPYR1 E141T
<400> 243
Met Pro Tyr Ala Ala Thr Arg Thr Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Ala Ser Asn Gly Arg Ala Val Ala Ala Cys Ala Gly His Ala Gly Val
20 25 30
Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala Ala Gly Gln
35 40 45
Cys Cys Ser Val Met Val Gln Ser Ile Ala Ala Pro Ala Asp Ala Val
50 55 60
Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Gly Tyr Lys Arg Phe
65 70 75 80
Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Val Glu Val Gly Ser
85 90 95
Val Arg Glu Leu Leu Val Val Ser Gly Leu Pro Ala Glu Asn Ser Arg
100 105 110
Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile Ser Phe Arg
115 120 125
Ile Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr
130 135 140
Val His Glu Ala Ala Ser Glu Gly Gly Pro Leu Thr Met Val Val Thr
145 150 155 160
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Val Glu Glu Thr Arg
165 170 175
Ile Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Glu Asp
180 185 190
Thr Val Ile Arg Gln Gln Ala Met Ala Ala Pro Ala Ala Pro His Asn
195 200 205
Asp His Asn His Ser
210
<210> 244
<211> 200
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLa，同源于AtPYR1 E141T
<400> 244
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Leu Ser Gly Gly Gly Ala Lys Ala Ala Ser His Gly Ala Ser Cys Ala
20 25 30
Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala Ala Arg Ala
35 40 45
Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala Pro Val Gly
50 55 60
Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys
65 70 75 80
His Phe Ile Arg Ser Cys Arg Leu Val Gly Gly Gly Asp Val Ala Val
85 90 95
Gly Ser Val Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Thr Ser
100 105 110
Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser
115 120 125
Phe Arg Val Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val
130 135 140
Thr Thr Val His Glu Ala Gly Ala Gly Ala Gly Thr Gly Thr Val Val
145 150 155 160
Val Thr Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala Asp Glu
165 170 175
Thr Arg Val Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
180 185 190
Ala Arg Thr Ala Glu Arg Leu Ala
195 200
<210> 245
<211> 220
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLb，同源于AtPYR1 E141T
<400> 245
Met Pro Cys Ile Gln Ala Ser Ser Pro Gly Gly Met Pro His Gln His
1 5 10 15
Gly Arg Gly Arg Val Leu Gly Gly Gly Val Gly Cys Ala Ala Glu Val
20 25 30
Ala Ala Ala Val Ala Ala Ser Ala Gly Gly Met Arg Cys Gly Ala His
35 40 45
Asp Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala
50 55 60
Ala Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val Ala Ala
65 70 75 80
Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Val Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly
100 105 110
Gly Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala
115 120 125
Ala Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val
130 135 140
Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Arg Asn Tyr Leu
145 150 155 160
Ser Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr
165 170 175
Val Val Val Thr Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro
180 185 190
Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln
195 200 205
Ser Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
210 215 220
<210> 246
<211> 218
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLo，同源于AtPYR1 E141T
<400> 246
Met Pro Cys Leu Gln Ala Ser Ser Pro Gly Ser Met Pro Tyr Gln His
1 5 10 15
His Gly Arg Gly Val Gly Cys Ala Ala Glu Ala Gly Ala Ala Val Gly
20 25 30
Ala Ser Ala Gly Thr Gly Thr Arg Cys Gly Ala His Asp Gly Glu Val
35 40 45
Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Pro Gly Pro Gly
50 55 60
Arg Cys Cys Ser Ala Val Val Gln Arg Val Ala Ala Pro Ala Glu Ala
65 70 75 80
Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Ala Tyr Lys Arg
85 90 95
Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly Val Gly Thr
100 105 110
Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Ala Ser Ser Arg
115 120 125
Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu Ser Phe Arg
130 135 140
Val Val Gly Gly Glu His Arg Leu Gln Asn Tyr Leu Ser Val Thr Thr
145 150 155 160
Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val Val Val Thr
165 170 175
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu Asp Thr Arg
180 185 190
Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln Ser Leu Ala Thr
195 200 205
Thr Ala Glu Lys Leu Ala Leu Ala Ala Val
210 215
<210> 247
<211> 212
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLi，同源于AtPYR1 E141T
<400> 247
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Val Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Asp Pro Ala Ile Cys Thr Val Val Leu Thr Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 248
<211> 205
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLk，同源于AtPYR1 E141T
<400> 248
Met Asp Gln Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Pro Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Val
65 70 75 80
Arg Pro Asp Pro Asp Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg
85 90 95
Glu Val Cys Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp His Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Gly Pro Gly Ile Cys Thr Val Val Leu Thr Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Ser Thr Ser Ser Ser Ala Pro Pro Pro Pro Ser Glu
195 200 205
<210> 249
<211> 188
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLj，同源于AtPYR1 E141T
<400> 249
Met Glu Pro His Met Glu Ser Ala Leu Arg Gln Gly Leu Ser Glu Ala
1 5 10 15
Glu Gln Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro
20 25 30
Gly Arg Ala Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp
35 40 45
Ala Pro Leu Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro
50 55 60
Gln Arg Tyr Lys His Phe Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp
65 70 75 80
Gly Ala Thr Val Gly Ser Val Arg Glu Val Thr Val Val Ser Gly Leu
85 90 95
Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg
100 105 110
His Ile Leu Ser Phe Arg Val Val Gly Gly Asp His Arg Leu Arg Asn
115 120 125
Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys
130 135 140
Val Val Leu Thr Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu
145 150 155 160
Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln
165 170 175
Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn
180 185
<210> 250
<211> 214
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLd，同源于AtPYR1 E141T
<400> 250
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Ala Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Pro Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Phe Val Gly Gly Asp His Arg
130 135 140
Leu Gln Asn Tyr Ser Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly
145 150 155 160
Pro Tyr Cys Val Val Leu Thr Ser Tyr Val Val Asp Val Pro Asp Gly
165 170 175
Asn Thr Glu Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu
180 185 190
Asn Leu Gln Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln
195 200 205
Thr Glu Pro Leu Asp Arg
210
<210> 251
<211> 209
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLf，同源于AtPYR1 E141T
<400> 251
Met Val Met Val Glu Met Asp Gly Gly Val Gly Gly Gly Gly Gly Gly
1 5 10 15
Gly Gln Thr Pro Ala Pro Arg Arg Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His
35 40 45
Glu Pro Arg Glu His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys
50 55 60
Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu Met Lys Gly Asn Ile
85 90 95
Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala
100 105 110
Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile
115 120 125
Leu Ser Val Arg Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser
130 135 140
Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys Val Val
145 150 155 160
Leu Thr Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu Glu Asp
165 170 175
Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu
180 185 190
Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln Thr Glu Pro Leu Asp
195 200 205
Arg
<210> 252
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLe，同源于AtPYR1 E141T
<400> 252
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His
20 25 30
Ala Pro Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Arg Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Thr Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Asn Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Leu Ile Asp Gln
195
<210> 253
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLg，同源于AtPYR1 E141T
<400> 253
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Thr Glu Tyr Val Arg Arg Leu His Arg His
20 25 30
Ala Pro Ala Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Ser Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Thr Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Lys Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Pro Ile Asp Gln
195
<210> 254
<211> 233
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLc，同源于AtPYR1 E141V
<400> 254
Met Arg Glu Arg Asn Ser Ser Ile Asp Gln Glu His Gln Arg Gly Ser
1 5 10 15
Ser Ser Arg Ser Thr Met Pro Phe Ala Ala Ser Arg Thr Ser Gln Gln
20 25 30
Gln His Ser Arg Val Ala Thr Asn Gly Arg Ala Val Ala Val Cys Ala
35 40 45
Gly His Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala
50 55 60
Val Ala Ala Gly Gln Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala
65 70 75 80
Pro Val Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Arg Tyr Lys Arg Phe Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly
100 105 110
Ala Glu Val Gly Ser Val Arg Glu Leu Leu Leu Val Ser Gly Leu Pro
115 120 125
Ala Glu Ser Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg
130 135 140
Val Ile Ser Phe Arg Val Leu Gly Gly Asp His Arg Leu Ala Asn Tyr
145 150 155 160
Arg Ser Val Thr Thr Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg
165 170 175
Pro Leu Thr Met Val Val Val Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Val Glu Glu Thr Arg Ile Phe Val Asp Thr Ile Val Arg Cys
195 200 205
Asn Leu Gln Ser Leu Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala
210 215 220
Ala Met Pro His Asp Asp Asn Gln Asn
225 230
<210> 255
<211> 213
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLl，同源于AtPYR1 E141V
<400> 255
Met Pro Tyr Ala Ala Thr Arg Thr Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Ala Ser Asn Gly Arg Ala Val Ala Ala Cys Ala Gly His Ala Gly Val
20 25 30
Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala Ala Gly Gln
35 40 45
Cys Cys Ser Val Met Val Gln Ser Ile Ala Ala Pro Ala Asp Ala Val
50 55 60
Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Gly Tyr Lys Arg Phe
65 70 75 80
Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Val Glu Val Gly Ser
85 90 95
Val Arg Glu Leu Leu Val Val Ser Gly Leu Pro Ala Glu Asn Ser Arg
100 105 110
Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile Ser Phe Arg
115 120 125
Ile Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr
130 135 140
Val His Glu Ala Ala Ser Glu Gly Gly Pro Leu Thr Met Val Val Val
145 150 155 160
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Val Glu Glu Thr Arg
165 170 175
Ile Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Glu Asp
180 185 190
Thr Val Ile Arg Gln Gln Ala Met Ala Ala Pro Ala Ala Pro His Asn
195 200 205
Asp His Asn His Ser
210
<210> 256
<211> 200
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLa，同源于AtPYR1 E141V
<400> 256
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Leu Ser Gly Gly Gly Ala Lys Ala Ala Ser His Gly Ala Ser Cys Ala
20 25 30
Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala Ala Arg Ala
35 40 45
Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala Pro Val Gly
50 55 60
Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys
65 70 75 80
His Phe Ile Arg Ser Cys Arg Leu Val Gly Gly Gly Asp Val Ala Val
85 90 95
Gly Ser Val Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Thr Ser
100 105 110
Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser
115 120 125
Phe Arg Val Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val
130 135 140
Thr Thr Val His Glu Ala Gly Ala Gly Ala Gly Thr Gly Thr Val Val
145 150 155 160
Val Val Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala Asp Glu
165 170 175
Thr Arg Val Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
180 185 190
Ala Arg Thr Ala Glu Arg Leu Ala
195 200
<210> 257
<211> 220
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLb，同源于AtPYR1 E141V
<400> 257
Met Pro Cys Ile Gln Ala Ser Ser Pro Gly Gly Met Pro His Gln His
1 5 10 15
Gly Arg Gly Arg Val Leu Gly Gly Gly Val Gly Cys Ala Ala Glu Val
20 25 30
Ala Ala Ala Val Ala Ala Ser Ala Gly Gly Met Arg Cys Gly Ala His
35 40 45
Asp Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala
50 55 60
Ala Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val Ala Ala
65 70 75 80
Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Val Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly
100 105 110
Gly Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala
115 120 125
Ala Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val
130 135 140
Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Arg Asn Tyr Leu
145 150 155 160
Ser Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr
165 170 175
Val Val Val Val Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro
180 185 190
Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln
195 200 205
Ser Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
210 215 220
<210> 258
<211> 218
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLo，同源于AtPYR1 E141V
<400> 258
Met Pro Cys Leu Gln Ala Ser Ser Pro Gly Ser Met Pro Tyr Gln His
1 5 10 15
His Gly Arg Gly Val Gly Cys Ala Ala Glu Ala Gly Ala Ala Val Gly
20 25 30
Ala Ser Ala Gly Thr Gly Thr Arg Cys Gly Ala His Asp Gly Glu Val
35 40 45
Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Pro Gly Pro Gly
50 55 60
Arg Cys Cys Ser Ala Val Val Gln Arg Val Ala Ala Pro Ala Glu Ala
65 70 75 80
Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Ala Tyr Lys Arg
85 90 95
Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly Val Gly Thr
100 105 110
Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Ala Ser Ser Arg
115 120 125
Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu Ser Phe Arg
130 135 140
Val Val Gly Gly Glu His Arg Leu Gln Asn Tyr Leu Ser Val Thr Thr
145 150 155 160
Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val Val Val Val
165 170 175
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu Asp Thr Arg
180 185 190
Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln Ser Leu Ala Thr
195 200 205
Thr Ala Glu Lys Leu Ala Leu Ala Ala Val
210 215
<210> 259
<211> 212
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLi，同源于AtPYR1 E141V
<400> 259
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Val Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Asp Pro Ala Ile Cys Thr Val Val Leu Val Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 260
<211> 205
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLk，同源于AtPYR1 E141V
<400> 260
Met Asp Gln Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Pro Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Val
65 70 75 80
Arg Pro Asp Pro Asp Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg
85 90 95
Glu Val Cys Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp His Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Gly Pro Gly Ile Cys Thr Val Val Leu Val Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Ser Thr Ser Ser Ser Ala Pro Pro Pro Pro Ser Glu
195 200 205
<210> 261
<211> 188
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLj，同源于AtPYR1 E141V
<400> 261
Met Glu Pro His Met Glu Ser Ala Leu Arg Gln Gly Leu Ser Glu Ala
1 5 10 15
Glu Gln Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro
20 25 30
Gly Arg Ala Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp
35 40 45
Ala Pro Leu Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro
50 55 60
Gln Arg Tyr Lys His Phe Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp
65 70 75 80
Gly Ala Thr Val Gly Ser Val Arg Glu Val Thr Val Val Ser Gly Leu
85 90 95
Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg
100 105 110
His Ile Leu Ser Phe Arg Val Val Gly Gly Asp His Arg Leu Arg Asn
115 120 125
Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys
130 135 140
Val Val Leu Val Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu
145 150 155 160
Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln
165 170 175
Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn
180 185
<210> 262
<211> 214
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLd，同源于AtPYR1 E141V
<400> 262
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Ala Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Pro Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Phe Val Gly Gly Asp His Arg
130 135 140
Leu Gln Asn Tyr Ser Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly
145 150 155 160
Pro Tyr Cys Val Val Leu Val Ser Tyr Val Val Asp Val Pro Asp Gly
165 170 175
Asn Thr Glu Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu
180 185 190
Asn Leu Gln Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln
195 200 205
Thr Glu Pro Leu Asp Arg
210
<210> 263
<211> 209
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLf，同源于AtPYR1 E141V
<400> 263
Met Val Met Val Glu Met Asp Gly Gly Val Gly Gly Gly Gly Gly Gly
1 5 10 15
Gly Gln Thr Pro Ala Pro Arg Arg Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His
35 40 45
Glu Pro Arg Glu His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys
50 55 60
Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu Met Lys Gly Asn Ile
85 90 95
Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala
100 105 110
Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile
115 120 125
Leu Ser Val Arg Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser
130 135 140
Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys Val Val
145 150 155 160
Leu Val Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu Glu Asp
165 170 175
Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu
180 185 190
Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln Thr Glu Pro Leu Asp
195 200 205
Arg
<210> 264
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLe，同源于AtPYR1 E141V
<400> 264
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His
20 25 30
Ala Pro Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Arg Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Val Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Asn Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Leu Ile Asp Gln
195
<210> 265
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLg，同源于AtPYR1 E141V
<400> 265
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Thr Glu Tyr Val Arg Arg Leu His Arg His
20 25 30
Ala Pro Ala Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Ser Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Val Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Lys Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Pro Ile Asp Gln
195
<210> 266
<211> 233
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLc，同源于AtPYR1 E141W
<400> 266
Met Arg Glu Arg Asn Ser Ser Ile Asp Gln Glu His Gln Arg Gly Ser
1 5 10 15
Ser Ser Arg Ser Thr Met Pro Phe Ala Ala Ser Arg Thr Ser Gln Gln
20 25 30
Gln His Ser Arg Val Ala Thr Asn Gly Arg Ala Val Ala Val Cys Ala
35 40 45
Gly His Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala
50 55 60
Val Ala Ala Gly Gln Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala
65 70 75 80
Pro Val Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Arg Tyr Lys Arg Phe Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly
100 105 110
Ala Glu Val Gly Ser Val Arg Glu Leu Leu Leu Val Ser Gly Leu Pro
115 120 125
Ala Glu Ser Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg
130 135 140
Val Ile Ser Phe Arg Val Leu Gly Gly Asp His Arg Leu Ala Asn Tyr
145 150 155 160
Arg Ser Val Thr Thr Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg
165 170 175
Pro Leu Thr Met Val Val Trp Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Val Glu Glu Thr Arg Ile Phe Val Asp Thr Ile Val Arg Cys
195 200 205
Asn Leu Gln Ser Leu Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala
210 215 220
Ala Met Pro His Asp Asp Asn Gln Asn
225 230
<210> 267
<211> 213
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLl，同源于AtPYR1 E141W
<400> 267
Met Pro Tyr Ala Ala Thr Arg Thr Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Ala Ser Asn Gly Arg Ala Val Ala Ala Cys Ala Gly His Ala Gly Val
20 25 30
Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala Ala Gly Gln
35 40 45
Cys Cys Ser Val Met Val Gln Ser Ile Ala Ala Pro Ala Asp Ala Val
50 55 60
Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Gly Tyr Lys Arg Phe
65 70 75 80
Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Val Glu Val Gly Ser
85 90 95
Val Arg Glu Leu Leu Val Val Ser Gly Leu Pro Ala Glu Asn Ser Arg
100 105 110
Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile Ser Phe Arg
115 120 125
Ile Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr
130 135 140
Val His Glu Ala Ala Ser Glu Gly Gly Pro Leu Thr Met Val Val Trp
145 150 155 160
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Val Glu Glu Thr Arg
165 170 175
Ile Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Glu Asp
180 185 190
Thr Val Ile Arg Gln Gln Ala Met Ala Ala Pro Ala Ala Pro His Asn
195 200 205
Asp His Asn His Ser
210
<210> 268
<211> 200
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLa，同源于AtPYR1 E141W
<400> 268
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Leu Ser Gly Gly Gly Ala Lys Ala Ala Ser His Gly Ala Ser Cys Ala
20 25 30
Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala Ala Arg Ala
35 40 45
Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala Pro Val Gly
50 55 60
Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys
65 70 75 80
His Phe Ile Arg Ser Cys Arg Leu Val Gly Gly Gly Asp Val Ala Val
85 90 95
Gly Ser Val Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Thr Ser
100 105 110
Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser
115 120 125
Phe Arg Val Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val
130 135 140
Thr Thr Val His Glu Ala Gly Ala Gly Ala Gly Thr Gly Thr Val Val
145 150 155 160
Val Trp Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala Asp Glu
165 170 175
Thr Arg Val Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
180 185 190
Ala Arg Thr Ala Glu Arg Leu Ala
195 200
<210> 269
<211> 220
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLb，同源于AtPYR1 E141W
<400> 269
Met Pro Cys Ile Gln Ala Ser Ser Pro Gly Gly Met Pro His Gln His
1 5 10 15
Gly Arg Gly Arg Val Leu Gly Gly Gly Val Gly Cys Ala Ala Glu Val
20 25 30
Ala Ala Ala Val Ala Ala Ser Ala Gly Gly Met Arg Cys Gly Ala His
35 40 45
Asp Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala
50 55 60
Ala Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val Ala Ala
65 70 75 80
Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Val Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly
100 105 110
Gly Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala
115 120 125
Ala Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val
130 135 140
Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Arg Asn Tyr Leu
145 150 155 160
Ser Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr
165 170 175
Val Val Val Trp Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro
180 185 190
Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln
195 200 205
Ser Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
210 215 220
<210> 270
<211> 218
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLo，同源于AtPYR1 E141W
<400> 270
Met Pro Cys Leu Gln Ala Ser Ser Pro Gly Ser Met Pro Tyr Gln His
1 5 10 15
His Gly Arg Gly Val Gly Cys Ala Ala Glu Ala Gly Ala Ala Val Gly
20 25 30
Ala Ser Ala Gly Thr Gly Thr Arg Cys Gly Ala His Asp Gly Glu Val
35 40 45
Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Pro Gly Pro Gly
50 55 60
Arg Cys Cys Ser Ala Val Val Gln Arg Val Ala Ala Pro Ala Glu Ala
65 70 75 80
Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Ala Tyr Lys Arg
85 90 95
Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly Val Gly Thr
100 105 110
Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Ala Ser Ser Arg
115 120 125
Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu Ser Phe Arg
130 135 140
Val Val Gly Gly Glu His Arg Leu Gln Asn Tyr Leu Ser Val Thr Thr
145 150 155 160
Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val Val Val Trp
165 170 175
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu Asp Thr Arg
180 185 190
Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln Ser Leu Ala Thr
195 200 205
Thr Ala Glu Lys Leu Ala Leu Ala Ala Val
210 215
<210> 271
<211> 212
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLi，同源于AtPYR1 E141W
<400> 271
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Val Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Asp Pro Ala Ile Cys Thr Val Val Leu Trp Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 272
<211> 205
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLk，同源于AtPYR1 E141W
<400> 272
Met Asp Gln Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Pro Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Val
65 70 75 80
Arg Pro Asp Pro Asp Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg
85 90 95
Glu Val Cys Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp His Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Gly Pro Gly Ile Cys Thr Val Val Leu Trp Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Ser Thr Ser Ser Ser Ala Pro Pro Pro Pro Ser Glu
195 200 205
<210> 273
<211> 188
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLj，同源于AtPYR1 E141W
<400> 273
Met Glu Pro His Met Glu Ser Ala Leu Arg Gln Gly Leu Ser Glu Ala
1 5 10 15
Glu Gln Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro
20 25 30
Gly Arg Ala Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp
35 40 45
Ala Pro Leu Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro
50 55 60
Gln Arg Tyr Lys His Phe Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp
65 70 75 80
Gly Ala Thr Val Gly Ser Val Arg Glu Val Thr Val Val Ser Gly Leu
85 90 95
Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg
100 105 110
His Ile Leu Ser Phe Arg Val Val Gly Gly Asp His Arg Leu Arg Asn
115 120 125
Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys
130 135 140
Val Val Leu Trp Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu
145 150 155 160
Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln
165 170 175
Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn
180 185
<210> 274
<211> 214
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLd，同源于AtPYR1 E141W
<400> 274
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Ala Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Pro Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Phe Val Gly Gly Asp His Arg
130 135 140
Leu Gln Asn Tyr Ser Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly
145 150 155 160
Pro Tyr Cys Val Val Leu Trp Ser Tyr Val Val Asp Val Pro Asp Gly
165 170 175
Asn Thr Glu Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu
180 185 190
Asn Leu Gln Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln
195 200 205
Thr Glu Pro Leu Asp Arg
210
<210> 275
<211> 209
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLf，同源于AtPYR1 E141W
<400> 275
Met Val Met Val Glu Met Asp Gly Gly Val Gly Gly Gly Gly Gly Gly
1 5 10 15
Gly Gln Thr Pro Ala Pro Arg Arg Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His
35 40 45
Glu Pro Arg Glu His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys
50 55 60
Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu Met Lys Gly Asn Ile
85 90 95
Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala
100 105 110
Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile
115 120 125
Leu Ser Val Arg Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser
130 135 140
Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys Val Val
145 150 155 160
Leu Trp Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu Glu Asp
165 170 175
Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu
180 185 190
Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln Thr Glu Pro Leu Asp
195 200 205
Arg
<210> 276
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLe，同源于AtPYR1 E141W
<400> 276
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His
20 25 30
Ala Pro Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Arg Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Trp Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Asn Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Leu Ile Asp Gln
195
<210> 277
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLg，同源于AtPYR1 E141W
<400> 277
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Thr Glu Tyr Val Arg Arg Leu His Arg His
20 25 30
Ala Pro Ala Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Ser Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Trp Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Lys Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Pro Ile Asp Gln
195
<210> 278
<211> 233
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLc，同源于AtPYR1 E141Y
<400> 278
Met Arg Glu Arg Asn Ser Ser Ile Asp Gln Glu His Gln Arg Gly Ser
1 5 10 15
Ser Ser Arg Ser Thr Met Pro Phe Ala Ala Ser Arg Thr Ser Gln Gln
20 25 30
Gln His Ser Arg Val Ala Thr Asn Gly Arg Ala Val Ala Val Cys Ala
35 40 45
Gly His Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala
50 55 60
Val Ala Ala Gly Gln Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala
65 70 75 80
Pro Val Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Arg Tyr Lys Arg Phe Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly
100 105 110
Ala Glu Val Gly Ser Val Arg Glu Leu Leu Leu Val Ser Gly Leu Pro
115 120 125
Ala Glu Ser Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg
130 135 140
Val Ile Ser Phe Arg Val Leu Gly Gly Asp His Arg Leu Ala Asn Tyr
145 150 155 160
Arg Ser Val Thr Thr Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg
165 170 175
Pro Leu Thr Met Val Val Tyr Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Val Glu Glu Thr Arg Ile Phe Val Asp Thr Ile Val Arg Cys
195 200 205
Asn Leu Gln Ser Leu Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala
210 215 220
Ala Met Pro His Asp Asp Asn Gln Asn
225 230
<210> 279
<211> 213
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLl，同源于AtPYR1 E141Y
<400> 279
Met Pro Tyr Ala Ala Thr Arg Thr Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Ala Ser Asn Gly Arg Ala Val Ala Ala Cys Ala Gly His Ala Gly Val
20 25 30
Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala Ala Gly Gln
35 40 45
Cys Cys Ser Val Met Val Gln Ser Ile Ala Ala Pro Ala Asp Ala Val
50 55 60
Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Gly Tyr Lys Arg Phe
65 70 75 80
Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Val Glu Val Gly Ser
85 90 95
Val Arg Glu Leu Leu Val Val Ser Gly Leu Pro Ala Glu Asn Ser Arg
100 105 110
Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile Ser Phe Arg
115 120 125
Ile Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr
130 135 140
Val His Glu Ala Ala Ser Glu Gly Gly Pro Leu Thr Met Val Val Tyr
145 150 155 160
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Val Glu Glu Thr Arg
165 170 175
Ile Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Glu Asp
180 185 190
Thr Val Ile Arg Gln Gln Ala Met Ala Ala Pro Ala Ala Pro His Asn
195 200 205
Asp His Asn His Ser
210
<210> 280
<211> 200
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLa，同源于AtPYR1 E141Y
<400> 280
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Leu Ser Gly Gly Gly Ala Lys Ala Ala Ser His Gly Ala Ser Cys Ala
20 25 30
Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala Ala Arg Ala
35 40 45
Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala Pro Val Gly
50 55 60
Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys
65 70 75 80
His Phe Ile Arg Ser Cys Arg Leu Val Gly Gly Gly Asp Val Ala Val
85 90 95
Gly Ser Val Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Thr Ser
100 105 110
Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser
115 120 125
Phe Arg Val Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val
130 135 140
Thr Thr Val His Glu Ala Gly Ala Gly Ala Gly Thr Gly Thr Val Val
145 150 155 160
Val Tyr Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala Asp Glu
165 170 175
Thr Arg Val Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
180 185 190
Ala Arg Thr Ala Glu Arg Leu Ala
195 200
<210> 281
<211> 220
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLb，同源于AtPYR1 E141Y
<400> 281
Met Pro Cys Ile Gln Ala Ser Ser Pro Gly Gly Met Pro His Gln His
1 5 10 15
Gly Arg Gly Arg Val Leu Gly Gly Gly Val Gly Cys Ala Ala Glu Val
20 25 30
Ala Ala Ala Val Ala Ala Ser Ala Gly Gly Met Arg Cys Gly Ala His
35 40 45
Asp Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala
50 55 60
Ala Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val Ala Ala
65 70 75 80
Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Val Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly
100 105 110
Gly Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala
115 120 125
Ala Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val
130 135 140
Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Arg Asn Tyr Leu
145 150 155 160
Ser Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr
165 170 175
Val Val Val Tyr Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro
180 185 190
Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln
195 200 205
Ser Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
210 215 220
<210> 282
<211> 218
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLo，同源于AtPYR1 E141Y
<400> 282
Met Pro Cys Leu Gln Ala Ser Ser Pro Gly Ser Met Pro Tyr Gln His
1 5 10 15
His Gly Arg Gly Val Gly Cys Ala Ala Glu Ala Gly Ala Ala Val Gly
20 25 30
Ala Ser Ala Gly Thr Gly Thr Arg Cys Gly Ala His Asp Gly Glu Val
35 40 45
Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Pro Gly Pro Gly
50 55 60
Arg Cys Cys Ser Ala Val Val Gln Arg Val Ala Ala Pro Ala Glu Ala
65 70 75 80
Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Ala Tyr Lys Arg
85 90 95
Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly Val Gly Thr
100 105 110
Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Ala Ser Ser Arg
115 120 125
Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu Ser Phe Arg
130 135 140
Val Val Gly Gly Glu His Arg Leu Gln Asn Tyr Leu Ser Val Thr Thr
145 150 155 160
Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val Val Val Tyr
165 170 175
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu Asp Thr Arg
180 185 190
Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln Ser Leu Ala Thr
195 200 205
Thr Ala Glu Lys Leu Ala Leu Ala Ala Val
210 215
<210> 283
<211> 212
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLi，同源于AtPYR1 E141Y
<400> 283
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Val Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Asp Pro Ala Ile Cys Thr Val Val Leu Tyr Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 284
<211> 205
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLk，同源于AtPYR1 E141Y
<400> 284
Met Asp Gln Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Pro Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Val
65 70 75 80
Arg Pro Asp Pro Asp Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg
85 90 95
Glu Val Cys Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp His Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Gly Pro Gly Ile Cys Thr Val Val Leu Tyr Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Ser Thr Ser Ser Ser Ala Pro Pro Pro Pro Ser Glu
195 200 205
<210> 285
<211> 188
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLj，同源于AtPYR1 E141Y
<400> 285
Met Glu Pro His Met Glu Ser Ala Leu Arg Gln Gly Leu Ser Glu Ala
1 5 10 15
Glu Gln Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro
20 25 30
Gly Arg Ala Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp
35 40 45
Ala Pro Leu Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro
50 55 60
Gln Arg Tyr Lys His Phe Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp
65 70 75 80
Gly Ala Thr Val Gly Ser Val Arg Glu Val Thr Val Val Ser Gly Leu
85 90 95
Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg
100 105 110
His Ile Leu Ser Phe Arg Val Val Gly Gly Asp His Arg Leu Arg Asn
115 120 125
Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys
130 135 140
Val Val Leu Tyr Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu
145 150 155 160
Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln
165 170 175
Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn
180 185
<210> 286
<211> 214
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLd，同源于AtPYR1 E141Y
<400> 286
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Ala Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Pro Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Phe Val Gly Gly Asp His Arg
130 135 140
Leu Gln Asn Tyr Ser Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly
145 150 155 160
Pro Tyr Cys Val Val Leu Tyr Ser Tyr Val Val Asp Val Pro Asp Gly
165 170 175
Asn Thr Glu Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu
180 185 190
Asn Leu Gln Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln
195 200 205
Thr Glu Pro Leu Asp Arg
210
<210> 287
<211> 209
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLf，同源于AtPYR1 E141Y
<400> 287
Met Val Met Val Glu Met Asp Gly Gly Val Gly Gly Gly Gly Gly Gly
1 5 10 15
Gly Gln Thr Pro Ala Pro Arg Arg Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His
35 40 45
Glu Pro Arg Glu His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys
50 55 60
Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu Met Lys Gly Asn Ile
85 90 95
Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala
100 105 110
Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile
115 120 125
Leu Ser Val Arg Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser
130 135 140
Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys Val Val
145 150 155 160
Leu Tyr Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu Glu Asp
165 170 175
Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu
180 185 190
Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln Thr Glu Pro Leu Asp
195 200 205
Arg
<210> 288
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLe，同源于AtPYR1 E141Y
<400> 288
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His
20 25 30
Ala Pro Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Arg Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Tyr Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Asn Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Leu Ile Asp Gln
195
<210> 289
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLg，同源于AtPYR1 E141Y
<400> 289
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Thr Glu Tyr Val Arg Arg Leu His Arg His
20 25 30
Ala Pro Ala Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Ser Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Tyr Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Lys Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Pro Ile Asp Gln
195
<210> 290
<211> 233
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLc，同源于AtPYR1 F61L
<400> 290
Met Arg Glu Arg Asn Ser Ser Ile Asp Gln Glu His Gln Arg Gly Ser
1 5 10 15
Ser Ser Arg Ser Thr Met Pro Phe Ala Ala Ser Arg Thr Ser Gln Gln
20 25 30
Gln His Ser Arg Val Ala Thr Asn Gly Arg Ala Val Ala Val Cys Ala
35 40 45
Gly His Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala
50 55 60
Val Ala Ala Gly Gln Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala
65 70 75 80
Pro Val Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Arg Tyr Lys Arg Leu Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly
100 105 110
Ala Glu Val Gly Ser Val Arg Glu Leu Leu Leu Val Ser Gly Leu Pro
115 120 125
Ala Glu Ser Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg
130 135 140
Val Ile Ser Phe Arg Val Leu Gly Gly Asp His Arg Leu Ala Asn Tyr
145 150 155 160
Arg Ser Val Thr Thr Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg
165 170 175
Pro Leu Thr Met Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Val Glu Glu Thr Arg Ile Phe Val Asp Thr Ile Val Arg Cys
195 200 205
Asn Leu Gln Ser Leu Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala
210 215 220
Ala Met Pro His Asp Asp Asn Gln Asn
225 230
<210> 291
<211> 213
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLl，同源于AtPYR1 F61L
<400> 291
Met Pro Tyr Ala Ala Thr Arg Thr Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Ala Ser Asn Gly Arg Ala Val Ala Ala Cys Ala Gly His Ala Gly Val
20 25 30
Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala Ala Gly Gln
35 40 45
Cys Cys Ser Val Met Val Gln Ser Ile Ala Ala Pro Ala Asp Ala Val
50 55 60
Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Gly Tyr Lys Arg Leu
65 70 75 80
Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Val Glu Val Gly Ser
85 90 95
Val Arg Glu Leu Leu Val Val Ser Gly Leu Pro Ala Glu Asn Ser Arg
100 105 110
Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile Ser Phe Arg
115 120 125
Ile Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr
130 135 140
Val His Glu Ala Ala Ser Glu Gly Gly Pro Leu Thr Met Val Val Glu
145 150 155 160
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Val Glu Glu Thr Arg
165 170 175
Ile Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Glu Asp
180 185 190
Thr Val Ile Arg Gln Gln Ala Met Ala Ala Pro Ala Ala Pro His Asn
195 200 205
Asp His Asn His Ser
210
<210> 292
<211> 200
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLa，同源于AtPYR1 F61L
<400> 292
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Leu Ser Gly Gly Gly Ala Lys Ala Ala Ser His Gly Ala Ser Cys Ala
20 25 30
Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala Ala Arg Ala
35 40 45
Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala Pro Val Gly
50 55 60
Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys
65 70 75 80
His Leu Ile Arg Ser Cys Arg Leu Val Gly Gly Gly Asp Val Ala Val
85 90 95
Gly Ser Val Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Thr Ser
100 105 110
Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser
115 120 125
Phe Arg Val Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val
130 135 140
Thr Thr Val His Glu Ala Gly Ala Gly Ala Gly Thr Gly Thr Val Val
145 150 155 160
Val Glu Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala Asp Glu
165 170 175
Thr Arg Val Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
180 185 190
Ala Arg Thr Ala Glu Arg Leu Ala
195 200
<210> 293
<211> 220
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLb，同源于AtPYR1 F61L
<400> 293
Met Pro Cys Ile Gln Ala Ser Ser Pro Gly Gly Met Pro His Gln His
1 5 10 15
Gly Arg Gly Arg Val Leu Gly Gly Gly Val Gly Cys Ala Ala Glu Val
20 25 30
Ala Ala Ala Val Ala Ala Ser Ala Gly Gly Met Arg Cys Gly Ala His
35 40 45
Asp Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala
50 55 60
Ala Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val Ala Ala
65 70 75 80
Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Val Tyr Lys Arg Leu Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly
100 105 110
Gly Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala
115 120 125
Ala Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val
130 135 140
Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Arg Asn Tyr Leu
145 150 155 160
Ser Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr
165 170 175
Val Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro
180 185 190
Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln
195 200 205
Ser Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
210 215 220
<210> 294
<211> 218
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLo，同源于AtPYR1 F61L
<400> 294
Met Pro Cys Leu Gln Ala Ser Ser Pro Gly Ser Met Pro Tyr Gln His
1 5 10 15
His Gly Arg Gly Val Gly Cys Ala Ala Glu Ala Gly Ala Ala Val Gly
20 25 30
Ala Ser Ala Gly Thr Gly Thr Arg Cys Gly Ala His Asp Gly Glu Val
35 40 45
Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Pro Gly Pro Gly
50 55 60
Arg Cys Cys Ser Ala Val Val Gln Arg Val Ala Ala Pro Ala Glu Ala
65 70 75 80
Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Ala Tyr Lys Arg
85 90 95
Leu Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly Val Gly Thr
100 105 110
Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Ala Ser Ser Arg
115 120 125
Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu Ser Phe Arg
130 135 140
Val Val Gly Gly Glu His Arg Leu Gln Asn Tyr Leu Ser Val Thr Thr
145 150 155 160
Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val Val Val Glu
165 170 175
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu Asp Thr Arg
180 185 190
Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln Ser Leu Ala Thr
195 200 205
Thr Ala Glu Lys Leu Ala Leu Ala Ala Val
210 215
<210> 295
<211> 212
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLi，同源于AtPYR1 F61L
<400> 295
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Leu Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Val Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Asp Pro Ala Ile Cys Thr Val Val Leu Glu Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 296
<211> 205
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLk，同源于AtPYR1 F61L
<400> 296
Met Asp Gln Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Pro Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Leu Ile Arg Ser Cys Ala Val
65 70 75 80
Arg Pro Asp Pro Asp Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg
85 90 95
Glu Val Cys Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp His Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Gly Pro Gly Ile Cys Thr Val Val Leu Glu Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Ser Thr Ser Ser Ser Ala Pro Pro Pro Pro Ser Glu
195 200 205
<210> 297
<211> 188
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLj，同源于AtPYR1 F61L
<400> 297
Met Glu Pro His Met Glu Ser Ala Leu Arg Gln Gly Leu Ser Glu Ala
1 5 10 15
Glu Gln Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro
20 25 30
Gly Arg Ala Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp
35 40 45
Ala Pro Leu Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro
50 55 60
Gln Arg Tyr Lys His Leu Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp
65 70 75 80
Gly Ala Thr Val Gly Ser Val Arg Glu Val Thr Val Val Ser Gly Leu
85 90 95
Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg
100 105 110
His Ile Leu Ser Phe Arg Val Val Gly Gly Asp His Arg Leu Arg Asn
115 120 125
Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys
130 135 140
Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu
145 150 155 160
Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln
165 170 175
Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn
180 185
<210> 298
<211> 214
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLd，同源于AtPYR1 F61L
<400> 298
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Ala Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Pro Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Leu Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Phe Val Gly Gly Asp His Arg
130 135 140
Leu Gln Asn Tyr Ser Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly
145 150 155 160
Pro Tyr Cys Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly
165 170 175
Asn Thr Glu Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu
180 185 190
Asn Leu Gln Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln
195 200 205
Thr Glu Pro Leu Asp Arg
210
<210> 299
<211> 209
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLf，同源于AtPYR1 F61L
<400> 299
Met Val Met Val Glu Met Asp Gly Gly Val Gly Gly Gly Gly Gly Gly
1 5 10 15
Gly Gln Thr Pro Ala Pro Arg Arg Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His
35 40 45
Glu Pro Arg Glu His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys
50 55 60
Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Leu Val Ser Arg Cys Glu Met Lys Gly Asn Ile
85 90 95
Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala
100 105 110
Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile
115 120 125
Leu Ser Val Arg Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser
130 135 140
Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys Val Val
145 150 155 160
Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu Glu Asp
165 170 175
Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu
180 185 190
Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln Thr Glu Pro Leu Asp
195 200 205
Arg
<210> 300
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLe，同源于AtPYR1 F61L
<400> 300
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His
20 25 30
Ala Pro Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Arg Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Leu Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Asn Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Leu Ile Asp Gln
195
<210> 301
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLg，同源于AtPYR1 F61L
<400> 301
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Thr Glu Tyr Val Arg Arg Leu His Arg His
20 25 30
Ala Pro Ala Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Ser Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Leu Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Lys Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Pro Ile Asp Gln
195
<210> 302
<211> 233
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLc，同源于AtPYR1 F61M
<400> 302
Met Arg Glu Arg Asn Ser Ser Ile Asp Gln Glu His Gln Arg Gly Ser
1 5 10 15
Ser Ser Arg Ser Thr Met Pro Phe Ala Ala Ser Arg Thr Ser Gln Gln
20 25 30
Gln His Ser Arg Val Ala Thr Asn Gly Arg Ala Val Ala Val Cys Ala
35 40 45
Gly His Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala
50 55 60
Val Ala Ala Gly Gln Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala
65 70 75 80
Pro Val Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Arg Tyr Lys Arg Met Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly
100 105 110
Ala Glu Val Gly Ser Val Arg Glu Leu Leu Leu Val Ser Gly Leu Pro
115 120 125
Ala Glu Ser Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg
130 135 140
Val Ile Ser Phe Arg Val Leu Gly Gly Asp His Arg Leu Ala Asn Tyr
145 150 155 160
Arg Ser Val Thr Thr Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg
165 170 175
Pro Leu Thr Met Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Val Glu Glu Thr Arg Ile Phe Val Asp Thr Ile Val Arg Cys
195 200 205
Asn Leu Gln Ser Leu Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala
210 215 220
Ala Met Pro His Asp Asp Asn Gln Asn
225 230
<210> 303
<211> 213
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLl，同源于AtPYR1 F61M
<400> 303
Met Pro Tyr Ala Ala Thr Arg Thr Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Ala Ser Asn Gly Arg Ala Val Ala Ala Cys Ala Gly His Ala Gly Val
20 25 30
Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala Ala Gly Gln
35 40 45
Cys Cys Ser Val Met Val Gln Ser Ile Ala Ala Pro Ala Asp Ala Val
50 55 60
Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Gly Tyr Lys Arg Met
65 70 75 80
Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Val Glu Val Gly Ser
85 90 95
Val Arg Glu Leu Leu Val Val Ser Gly Leu Pro Ala Glu Asn Ser Arg
100 105 110
Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile Ser Phe Arg
115 120 125
Ile Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr
130 135 140
Val His Glu Ala Ala Ser Glu Gly Gly Pro Leu Thr Met Val Val Glu
145 150 155 160
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Val Glu Glu Thr Arg
165 170 175
Ile Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Glu Asp
180 185 190
Thr Val Ile Arg Gln Gln Ala Met Ala Ala Pro Ala Ala Pro His Asn
195 200 205
Asp His Asn His Ser
210
<210> 304
<211> 200
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLa，同源于AtPYR1 F61M
<400> 304
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Leu Ser Gly Gly Gly Ala Lys Ala Ala Ser His Gly Ala Ser Cys Ala
20 25 30
Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala Ala Arg Ala
35 40 45
Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala Pro Val Gly
50 55 60
Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys
65 70 75 80
His Met Ile Arg Ser Cys Arg Leu Val Gly Gly Gly Asp Val Ala Val
85 90 95
Gly Ser Val Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Thr Ser
100 105 110
Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser
115 120 125
Phe Arg Val Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val
130 135 140
Thr Thr Val His Glu Ala Gly Ala Gly Ala Gly Thr Gly Thr Val Val
145 150 155 160
Val Glu Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala Asp Glu
165 170 175
Thr Arg Val Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
180 185 190
Ala Arg Thr Ala Glu Arg Leu Ala
195 200
<210> 305
<211> 220
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLb，同源于AtPYR1 F61M
<400> 305
Met Pro Cys Ile Gln Ala Ser Ser Pro Gly Gly Met Pro His Gln His
1 5 10 15
Gly Arg Gly Arg Val Leu Gly Gly Gly Val Gly Cys Ala Ala Glu Val
20 25 30
Ala Ala Ala Val Ala Ala Ser Ala Gly Gly Met Arg Cys Gly Ala His
35 40 45
Asp Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala
50 55 60
Ala Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val Ala Ala
65 70 75 80
Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Val Tyr Lys Arg Met Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly
100 105 110
Gly Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala
115 120 125
Ala Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val
130 135 140
Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Arg Asn Tyr Leu
145 150 155 160
Ser Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr
165 170 175
Val Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro
180 185 190
Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln
195 200 205
Ser Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
210 215 220
<210> 306
<211> 218
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLo，同源于AtPYR1 F61M
<400> 306
Met Pro Cys Leu Gln Ala Ser Ser Pro Gly Ser Met Pro Tyr Gln His
1 5 10 15
His Gly Arg Gly Val Gly Cys Ala Ala Glu Ala Gly Ala Ala Val Gly
20 25 30
Ala Ser Ala Gly Thr Gly Thr Arg Cys Gly Ala His Asp Gly Glu Val
35 40 45
Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Pro Gly Pro Gly
50 55 60
Arg Cys Cys Ser Ala Val Val Gln Arg Val Ala Ala Pro Ala Glu Ala
65 70 75 80
Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Ala Tyr Lys Arg
85 90 95
Met Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly Val Gly Thr
100 105 110
Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Ala Ser Ser Arg
115 120 125
Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu Ser Phe Arg
130 135 140
Val Val Gly Gly Glu His Arg Leu Gln Asn Tyr Leu Ser Val Thr Thr
145 150 155 160
Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val Val Val Glu
165 170 175
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu Asp Thr Arg
180 185 190
Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln Ser Leu Ala Thr
195 200 205
Thr Ala Glu Lys Leu Ala Leu Ala Ala Val
210 215
<210> 307
<211> 212
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLi，同源于AtPYR1 F61M
<400> 307
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Met Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Val Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Asp Pro Ala Ile Cys Thr Val Val Leu Glu Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 308
<211> 205
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLk，同源于AtPYR1 F61M
<400> 308
Met Asp Gln Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Pro Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Met Ile Arg Ser Cys Ala Val
65 70 75 80
Arg Pro Asp Pro Asp Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg
85 90 95
Glu Val Cys Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp His Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Gly Pro Gly Ile Cys Thr Val Val Leu Glu Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Ser Thr Ser Ser Ser Ala Pro Pro Pro Pro Ser Glu
195 200 205
<210> 309
<211> 188
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLj，同源于AtPYR1 F61M
<400> 309
Met Glu Pro His Met Glu Ser Ala Leu Arg Gln Gly Leu Ser Glu Ala
1 5 10 15
Glu Gln Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro
20 25 30
Gly Arg Ala Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp
35 40 45
Ala Pro Leu Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro
50 55 60
Gln Arg Tyr Lys His Met Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp
65 70 75 80
Gly Ala Thr Val Gly Ser Val Arg Glu Val Thr Val Val Ser Gly Leu
85 90 95
Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg
100 105 110
His Ile Leu Ser Phe Arg Val Val Gly Gly Asp His Arg Leu Arg Asn
115 120 125
Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys
130 135 140
Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu
145 150 155 160
Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln
165 170 175
Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn
180 185
<210> 310
<211> 214
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLd，同源于AtPYR1 F61M
<400> 310
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Ala Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Pro Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Met Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Phe Val Gly Gly Asp His Arg
130 135 140
Leu Gln Asn Tyr Ser Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly
145 150 155 160
Pro Tyr Cys Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly
165 170 175
Asn Thr Glu Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu
180 185 190
Asn Leu Gln Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln
195 200 205
Thr Glu Pro Leu Asp Arg
210
<210> 311
<211> 209
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLf，同源于AtPYR1 F61M
<400> 311
Met Val Met Val Glu Met Asp Gly Gly Val Gly Gly Gly Gly Gly Gly
1 5 10 15
Gly Gln Thr Pro Ala Pro Arg Arg Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His
35 40 45
Glu Pro Arg Glu His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys
50 55 60
Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Met Val Ser Arg Cys Glu Met Lys Gly Asn Ile
85 90 95
Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala
100 105 110
Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile
115 120 125
Leu Ser Val Arg Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser
130 135 140
Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys Val Val
145 150 155 160
Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu Glu Asp
165 170 175
Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu
180 185 190
Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln Thr Glu Pro Leu Asp
195 200 205
Arg
<210> 312
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLe，同源于AtPYR1 F61M
<400> 312
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His
20 25 30
Ala Pro Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Arg Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Met Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Asn Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Leu Ile Asp Gln
195
<210> 313
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLg，同源于AtPYR1 F61M
<400> 313
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Thr Glu Tyr Val Arg Arg Leu His Arg His
20 25 30
Ala Pro Ala Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Ser Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Met Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Lys Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Pro Ile Asp Gln
195
<210> 314
<211> 233
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLc，同源于AtPYR1 I110C
<400> 314
Met Arg Glu Arg Asn Ser Ser Ile Asp Gln Glu His Gln Arg Gly Ser
1 5 10 15
Ser Ser Arg Ser Thr Met Pro Phe Ala Ala Ser Arg Thr Ser Gln Gln
20 25 30
Gln His Ser Arg Val Ala Thr Asn Gly Arg Ala Val Ala Val Cys Ala
35 40 45
Gly His Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala
50 55 60
Val Ala Ala Gly Gln Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala
65 70 75 80
Pro Val Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Arg Tyr Lys Arg Phe Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly
100 105 110
Ala Glu Val Gly Ser Val Arg Glu Leu Leu Leu Val Ser Gly Leu Pro
115 120 125
Ala Glu Ser Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg
130 135 140
Val Ile Ser Phe Arg Cys Leu Gly Gly Asp His Arg Leu Ala Asn Tyr
145 150 155 160
Arg Ser Val Thr Thr Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg
165 170 175
Pro Leu Thr Met Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Val Glu Glu Thr Arg Ile Phe Val Asp Thr Ile Val Arg Cys
195 200 205
Asn Leu Gln Ser Leu Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala
210 215 220
Ala Met Pro His Asp Asp Asn Gln Asn
225 230
<210> 315
<211> 213
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLl，同源于AtPYR1 I110C
<400> 315
Met Pro Tyr Ala Ala Thr Arg Thr Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Ala Ser Asn Gly Arg Ala Val Ala Ala Cys Ala Gly His Ala Gly Val
20 25 30
Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala Ala Gly Gln
35 40 45
Cys Cys Ser Val Met Val Gln Ser Ile Ala Ala Pro Ala Asp Ala Val
50 55 60
Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Gly Tyr Lys Arg Phe
65 70 75 80
Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Val Glu Val Gly Ser
85 90 95
Val Arg Glu Leu Leu Val Val Ser Gly Leu Pro Ala Glu Asn Ser Arg
100 105 110
Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile Ser Phe Arg
115 120 125
Cys Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr
130 135 140
Val His Glu Ala Ala Ser Glu Gly Gly Pro Leu Thr Met Val Val Glu
145 150 155 160
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Val Glu Glu Thr Arg
165 170 175
Ile Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Glu Asp
180 185 190
Thr Val Ile Arg Gln Gln Ala Met Ala Ala Pro Ala Ala Pro His Asn
195 200 205
Asp His Asn His Ser
210
<210> 316
<211> 200
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLa，同源于AtPYR1 I110C
<400> 316
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Leu Ser Gly Gly Gly Ala Lys Ala Ala Ser His Gly Ala Ser Cys Ala
20 25 30
Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala Ala Arg Ala
35 40 45
Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala Pro Val Gly
50 55 60
Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys
65 70 75 80
His Phe Ile Arg Ser Cys Arg Leu Val Gly Gly Gly Asp Val Ala Val
85 90 95
Gly Ser Val Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Thr Ser
100 105 110
Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser
115 120 125
Phe Arg Cys Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val
130 135 140
Thr Thr Val His Glu Ala Gly Ala Gly Ala Gly Thr Gly Thr Val Val
145 150 155 160
Val Glu Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala Asp Glu
165 170 175
Thr Arg Val Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
180 185 190
Ala Arg Thr Ala Glu Arg Leu Ala
195 200
<210> 317
<211> 220
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLb，同源于AtPYR1 I110C
<400> 317
Met Pro Cys Ile Gln Ala Ser Ser Pro Gly Gly Met Pro His Gln His
1 5 10 15
Gly Arg Gly Arg Val Leu Gly Gly Gly Val Gly Cys Ala Ala Glu Val
20 25 30
Ala Ala Ala Val Ala Ala Ser Ala Gly Gly Met Arg Cys Gly Ala His
35 40 45
Asp Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala
50 55 60
Ala Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val Ala Ala
65 70 75 80
Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Val Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly
100 105 110
Gly Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala
115 120 125
Ala Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val
130 135 140
Leu Ser Phe Arg Cys Val Gly Gly Glu His Arg Leu Arg Asn Tyr Leu
145 150 155 160
Ser Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr
165 170 175
Val Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro
180 185 190
Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln
195 200 205
Ser Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
210 215 220
<210> 318
<211> 218
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLo，同源于AtPYR1 I110C
<400> 318
Met Pro Cys Leu Gln Ala Ser Ser Pro Gly Ser Met Pro Tyr Gln His
1 5 10 15
His Gly Arg Gly Val Gly Cys Ala Ala Glu Ala Gly Ala Ala Val Gly
20 25 30
Ala Ser Ala Gly Thr Gly Thr Arg Cys Gly Ala His Asp Gly Glu Val
35 40 45
Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Pro Gly Pro Gly
50 55 60
Arg Cys Cys Ser Ala Val Val Gln Arg Val Ala Ala Pro Ala Glu Ala
65 70 75 80
Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Ala Tyr Lys Arg
85 90 95
Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly Val Gly Thr
100 105 110
Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Ala Ser Ser Arg
115 120 125
Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu Ser Phe Arg
130 135 140
Cys Val Gly Gly Glu His Arg Leu Gln Asn Tyr Leu Ser Val Thr Thr
145 150 155 160
Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val Val Val Glu
165 170 175
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu Asp Thr Arg
180 185 190
Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln Ser Leu Ala Thr
195 200 205
Thr Ala Glu Lys Leu Ala Leu Ala Ala Val
210 215
<210> 319
<211> 212
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLi，同源于AtPYR1 I110C
<400> 319
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Val Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Cys Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Asp Pro Ala Ile Cys Thr Val Val Leu Glu Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 320
<211> 205
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLk，同源于AtPYR1 I110C
<400> 320
Met Asp Gln Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Pro Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Val
65 70 75 80
Arg Pro Asp Pro Asp Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg
85 90 95
Glu Val Cys Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp His Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Cys Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Gly Pro Gly Ile Cys Thr Val Val Leu Glu Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Ser Thr Ser Ser Ser Ala Pro Pro Pro Pro Ser Glu
195 200 205
<210> 321
<211> 188
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLj，同源于AtPYR1 I110C
<400> 321
Met Glu Pro His Met Glu Ser Ala Leu Arg Gln Gly Leu Ser Glu Ala
1 5 10 15
Glu Gln Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro
20 25 30
Gly Arg Ala Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp
35 40 45
Ala Pro Leu Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro
50 55 60
Gln Arg Tyr Lys His Phe Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp
65 70 75 80
Gly Ala Thr Val Gly Ser Val Arg Glu Val Thr Val Val Ser Gly Leu
85 90 95
Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg
100 105 110
His Ile Leu Ser Phe Arg Cys Val Gly Gly Asp His Arg Leu Arg Asn
115 120 125
Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys
130 135 140
Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu
145 150 155 160
Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln
165 170 175
Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn
180 185
<210> 322
<211> 214
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLd，同源于AtPYR1 I110C
<400> 322
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Ala Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Pro Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Cys Val Gly Gly Asp His Arg
130 135 140
Leu Gln Asn Tyr Ser Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly
145 150 155 160
Pro Tyr Cys Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly
165 170 175
Asn Thr Glu Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu
180 185 190
Asn Leu Gln Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln
195 200 205
Thr Glu Pro Leu Asp Arg
210
<210> 323
<211> 209
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLf，同源于AtPYR1 I110C
<400> 323
Met Val Met Val Glu Met Asp Gly Gly Val Gly Gly Gly Gly Gly Gly
1 5 10 15
Gly Gln Thr Pro Ala Pro Arg Arg Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His
35 40 45
Glu Pro Arg Glu His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys
50 55 60
Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu Met Lys Gly Asn Ile
85 90 95
Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala
100 105 110
Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile
115 120 125
Leu Ser Val Arg Cys Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser
130 135 140
Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys Val Val
145 150 155 160
Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu Glu Asp
165 170 175
Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu
180 185 190
Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln Thr Glu Pro Leu Asp
195 200 205
Arg
<210> 324
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLe，同源于AtPYR1 I110C
<400> 324
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His
20 25 30
Ala Pro Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Arg Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Cys Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Asn Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Leu Ile Asp Gln
195
<210> 325
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLg，同源于AtPYR1 I110C
<400> 325
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Thr Glu Tyr Val Arg Arg Leu His Arg His
20 25 30
Ala Pro Ala Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Ser Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Cys Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Lys Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Pro Ile Asp Gln
195
<210> 326
<211> 233
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLc，同源于AtPYR1 I110S
<400> 326
Met Arg Glu Arg Asn Ser Ser Ile Asp Gln Glu His Gln Arg Gly Ser
1 5 10 15
Ser Ser Arg Ser Thr Met Pro Phe Ala Ala Ser Arg Thr Ser Gln Gln
20 25 30
Gln His Ser Arg Val Ala Thr Asn Gly Arg Ala Val Ala Val Cys Ala
35 40 45
Gly His Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala
50 55 60
Val Ala Ala Gly Gln Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala
65 70 75 80
Pro Val Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Arg Tyr Lys Arg Phe Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly
100 105 110
Ala Glu Val Gly Ser Val Arg Glu Leu Leu Leu Val Ser Gly Leu Pro
115 120 125
Ala Glu Ser Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg
130 135 140
Val Ile Ser Phe Arg Ser Leu Gly Gly Asp His Arg Leu Ala Asn Tyr
145 150 155 160
Arg Ser Val Thr Thr Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg
165 170 175
Pro Leu Thr Met Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Val Glu Glu Thr Arg Ile Phe Val Asp Thr Ile Val Arg Cys
195 200 205
Asn Leu Gln Ser Leu Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala
210 215 220
Ala Met Pro His Asp Asp Asn Gln Asn
225 230
<210> 327
<211> 213
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLl，同源于AtPYR1 I110S
<400> 327
Met Pro Tyr Ala Ala Thr Arg Thr Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Ala Ser Asn Gly Arg Ala Val Ala Ala Cys Ala Gly His Ala Gly Val
20 25 30
Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala Ala Gly Gln
35 40 45
Cys Cys Ser Val Met Val Gln Ser Ile Ala Ala Pro Ala Asp Ala Val
50 55 60
Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Gly Tyr Lys Arg Phe
65 70 75 80
Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Val Glu Val Gly Ser
85 90 95
Val Arg Glu Leu Leu Val Val Ser Gly Leu Pro Ala Glu Asn Ser Arg
100 105 110
Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile Ser Phe Arg
115 120 125
Ser Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr
130 135 140
Val His Glu Ala Ala Ser Glu Gly Gly Pro Leu Thr Met Val Val Glu
145 150 155 160
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Val Glu Glu Thr Arg
165 170 175
Ile Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Glu Asp
180 185 190
Thr Val Ile Arg Gln Gln Ala Met Ala Ala Pro Ala Ala Pro His Asn
195 200 205
Asp His Asn His Ser
210
<210> 328
<211> 200
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLa，同源于AtPYR1 I110S
<400> 328
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Leu Ser Gly Gly Gly Ala Lys Ala Ala Ser His Gly Ala Ser Cys Ala
20 25 30
Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala Ala Arg Ala
35 40 45
Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala Pro Val Gly
50 55 60
Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys
65 70 75 80
His Phe Ile Arg Ser Cys Arg Leu Val Gly Gly Gly Asp Val Ala Val
85 90 95
Gly Ser Val Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Thr Ser
100 105 110
Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser
115 120 125
Phe Arg Ser Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val
130 135 140
Thr Thr Val His Glu Ala Gly Ala Gly Ala Gly Thr Gly Thr Val Val
145 150 155 160
Val Glu Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala Asp Glu
165 170 175
Thr Arg Val Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
180 185 190
Ala Arg Thr Ala Glu Arg Leu Ala
195 200
<210> 329
<211> 220
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLb，同源于AtPYR1 I110S
<400> 329
Met Pro Cys Ile Gln Ala Ser Ser Pro Gly Gly Met Pro His Gln His
1 5 10 15
Gly Arg Gly Arg Val Leu Gly Gly Gly Val Gly Cys Ala Ala Glu Val
20 25 30
Ala Ala Ala Val Ala Ala Ser Ala Gly Gly Met Arg Cys Gly Ala His
35 40 45
Asp Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala
50 55 60
Ala Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val Ala Ala
65 70 75 80
Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Val Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly
100 105 110
Gly Val Gly Thr Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala
115 120 125
Ala Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val
130 135 140
Leu Ser Phe Arg Ser Val Gly Gly Glu His Arg Leu Arg Asn Tyr Leu
145 150 155 160
Ser Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr
165 170 175
Val Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro
180 185 190
Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln
195 200 205
Ser Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
210 215 220
<210> 330
<211> 218
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLo，同源于AtPYR1 I110S
<400> 330
Met Pro Cys Leu Gln Ala Ser Ser Pro Gly Ser Met Pro Tyr Gln His
1 5 10 15
His Gly Arg Gly Val Gly Cys Ala Ala Glu Ala Gly Ala Ala Val Gly
20 25 30
Ala Ser Ala Gly Thr Gly Thr Arg Cys Gly Ala His Asp Gly Glu Val
35 40 45
Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Pro Gly Pro Gly
50 55 60
Arg Cys Cys Ser Ala Val Val Gln Arg Val Ala Ala Pro Ala Glu Ala
65 70 75 80
Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Ala Tyr Lys Arg
85 90 95
Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly Val Gly Thr
100 105 110
Leu Arg Glu Val Arg Val Val Ser Gly Leu Pro Ala Ala Ser Ser Arg
115 120 125
Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu Ser Phe Arg
130 135 140
Ser Val Gly Gly Glu His Arg Leu Gln Asn Tyr Leu Ser Val Thr Thr
145 150 155 160
Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val Val Val Glu
165 170 175
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu Asp Thr Arg
180 185 190
Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln Ser Leu Ala Thr
195 200 205
Thr Ala Glu Lys Leu Ala Leu Ala Ala Val
210 215
<210> 331
<211> 212
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLi，同源于AtPYR1 I110S
<400> 331
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Val Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ser Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Asp Pro Ala Ile Cys Thr Val Val Leu Glu Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 332
<211> 205
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLk，同源于AtPYR1 I110S
<400> 332
Met Asp Gln Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Pro Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Val
65 70 75 80
Arg Pro Asp Pro Asp Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg
85 90 95
Glu Val Cys Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp His Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ser Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Gly Pro Gly Ile Cys Thr Val Val Leu Glu Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Ser Thr Ser Ser Ser Ala Pro Pro Pro Pro Ser Glu
195 200 205
<210> 333
<211> 188
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLj，同源于AtPYR1 I110S
<400> 333
Met Glu Pro His Met Glu Ser Ala Leu Arg Gln Gly Leu Ser Glu Ala
1 5 10 15
Glu Gln Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro
20 25 30
Gly Arg Ala Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp
35 40 45
Ala Pro Leu Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro
50 55 60
Gln Arg Tyr Lys His Phe Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp
65 70 75 80
Gly Ala Thr Val Gly Ser Val Arg Glu Val Thr Val Val Ser Gly Leu
85 90 95
Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg
100 105 110
His Ile Leu Ser Phe Arg Ser Val Gly Gly Asp His Arg Leu Arg Asn
115 120 125
Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys
130 135 140
Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu
145 150 155 160
Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln
165 170 175
Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn
180 185
<210> 334
<211> 214
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLd，同源于AtPYR1 I110S
<400> 334
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Ala Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Pro Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Ser Val Gly Gly Asp His Arg
130 135 140
Leu Gln Asn Tyr Ser Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly
145 150 155 160
Pro Tyr Cys Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly
165 170 175
Asn Thr Glu Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu
180 185 190
Asn Leu Gln Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln
195 200 205
Thr Glu Pro Leu Asp Arg
210
<210> 335
<211> 209
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLf，同源于AtPYR1 I110S
<400> 335
Met Val Met Val Glu Met Asp Gly Gly Val Gly Gly Gly Gly Gly Gly
1 5 10 15
Gly Gln Thr Pro Ala Pro Arg Arg Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His
35 40 45
Glu Pro Arg Glu His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys
50 55 60
Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu Met Lys Gly Asn Ile
85 90 95
Glu Ile Gly Ser Val Arg Glu Val Asn Val Lys Ser Gly Leu Pro Ala
100 105 110
Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile
115 120 125
Leu Ser Val Arg Ser Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser
130 135 140
Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys Val Val
145 150 155 160
Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu Glu Asp
165 170 175
Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu
180 185 190
Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln Thr Glu Pro Leu Asp
195 200 205
Arg
<210> 336
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLe，同源于AtPYR1 I110S
<400> 336
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His
20 25 30
Ala Pro Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Arg Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Ser Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Asn Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Leu Ile Asp Gln
195
<210> 337
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLg，同源于AtPYR1 I110S
<400> 337
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Thr Glu Tyr Val Arg Arg Leu His Arg His
20 25 30
Ala Pro Ala Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Ser Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Val Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Ser Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Lys Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Pro Ile Asp Gln
195
<210> 338
<211> 233
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLc，同源于AtPYR1 V81I
<400> 338
Met Arg Glu Arg Asn Ser Ser Ile Asp Gln Glu His Gln Arg Gly Ser
1 5 10 15
Ser Ser Arg Ser Thr Met Pro Phe Ala Ala Ser Arg Thr Ser Gln Gln
20 25 30
Gln His Ser Arg Val Ala Thr Asn Gly Arg Ala Val Ala Val Cys Ala
35 40 45
Gly His Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala
50 55 60
Val Ala Ala Gly Gln Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala
65 70 75 80
Pro Val Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Arg Tyr Lys Arg Phe Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly
100 105 110
Ala Glu Val Gly Ser Val Arg Glu Ile Leu Leu Val Ser Gly Leu Pro
115 120 125
Ala Glu Ser Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg
130 135 140
Val Ile Ser Phe Arg Val Leu Gly Gly Asp His Arg Leu Ala Asn Tyr
145 150 155 160
Arg Ser Val Thr Thr Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg
165 170 175
Pro Leu Thr Met Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Val Glu Glu Thr Arg Ile Phe Val Asp Thr Ile Val Arg Cys
195 200 205
Asn Leu Gln Ser Leu Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala
210 215 220
Ala Met Pro His Asp Asp Asn Gln Asn
225 230
<210> 339
<211> 213
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLl，同源于AtPYR1 V81I
<400> 339
Met Pro Tyr Ala Ala Thr Arg Thr Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Ala Ser Asn Gly Arg Ala Val Ala Ala Cys Ala Gly His Ala Gly Val
20 25 30
Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala Ala Gly Gln
35 40 45
Cys Cys Ser Val Met Val Gln Ser Ile Ala Ala Pro Ala Asp Ala Val
50 55 60
Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Gly Tyr Lys Arg Phe
65 70 75 80
Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Val Glu Val Gly Ser
85 90 95
Val Arg Glu Ile Leu Val Val Ser Gly Leu Pro Ala Glu Asn Ser Arg
100 105 110
Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile Ser Phe Arg
115 120 125
Ile Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr
130 135 140
Val His Glu Ala Ala Ser Glu Gly Gly Pro Leu Thr Met Val Val Glu
145 150 155 160
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Val Glu Glu Thr Arg
165 170 175
Ile Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Glu Asp
180 185 190
Thr Val Ile Arg Gln Gln Ala Met Ala Ala Pro Ala Ala Pro His Asn
195 200 205
Asp His Asn His Ser
210
<210> 340
<211> 200
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLa，同源于AtPYR1 V81I
<400> 340
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Leu Ser Gly Gly Gly Ala Lys Ala Ala Ser His Gly Ala Ser Cys Ala
20 25 30
Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala Ala Arg Ala
35 40 45
Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala Pro Val Gly
50 55 60
Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys
65 70 75 80
His Phe Ile Arg Ser Cys Arg Leu Val Gly Gly Gly Asp Val Ala Val
85 90 95
Gly Ser Val Arg Glu Ile Arg Val Val Ser Gly Leu Pro Ala Thr Ser
100 105 110
Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser
115 120 125
Phe Arg Val Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val
130 135 140
Thr Thr Val His Glu Ala Gly Ala Gly Ala Gly Thr Gly Thr Val Val
145 150 155 160
Val Glu Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala Asp Glu
165 170 175
Thr Arg Val Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
180 185 190
Ala Arg Thr Ala Glu Arg Leu Ala
195 200
<210> 341
<211> 220
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLb，同源于AtPYR1 V81I
<400> 341
Met Pro Cys Ile Gln Ala Ser Ser Pro Gly Gly Met Pro His Gln His
1 5 10 15
Gly Arg Gly Arg Val Leu Gly Gly Gly Val Gly Cys Ala Ala Glu Val
20 25 30
Ala Ala Ala Val Ala Ala Ser Ala Gly Gly Met Arg Cys Gly Ala His
35 40 45
Asp Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala
50 55 60
Ala Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val Ala Ala
65 70 75 80
Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Val Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly
100 105 110
Gly Val Gly Thr Leu Arg Glu Ile Arg Val Val Ser Gly Leu Pro Ala
115 120 125
Ala Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val
130 135 140
Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Arg Asn Tyr Leu
145 150 155 160
Ser Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr
165 170 175
Val Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro
180 185 190
Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln
195 200 205
Ser Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
210 215 220
<210> 342
<211> 218
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLo，同源于AtPYR1 V81I
<400> 342
Met Pro Cys Leu Gln Ala Ser Ser Pro Gly Ser Met Pro Tyr Gln His
1 5 10 15
His Gly Arg Gly Val Gly Cys Ala Ala Glu Ala Gly Ala Ala Val Gly
20 25 30
Ala Ser Ala Gly Thr Gly Thr Arg Cys Gly Ala His Asp Gly Glu Val
35 40 45
Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Pro Gly Pro Gly
50 55 60
Arg Cys Cys Ser Ala Val Val Gln Arg Val Ala Ala Pro Ala Glu Ala
65 70 75 80
Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Ala Tyr Lys Arg
85 90 95
Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly Val Gly Thr
100 105 110
Leu Arg Glu Ile Arg Val Val Ser Gly Leu Pro Ala Ala Ser Ser Arg
115 120 125
Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu Ser Phe Arg
130 135 140
Val Val Gly Gly Glu His Arg Leu Gln Asn Tyr Leu Ser Val Thr Thr
145 150 155 160
Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val Val Val Glu
165 170 175
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu Asp Thr Arg
180 185 190
Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln Ser Leu Ala Thr
195 200 205
Thr Ala Glu Lys Leu Ala Leu Ala Ala Val
210 215
<210> 343
<211> 212
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLi，同源于AtPYR1 V81I
<400> 343
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Ile Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Asp Pro Ala Ile Cys Thr Val Val Leu Glu Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 344
<211> 205
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLk，同源于AtPYR1 V81I
<400> 344
Met Asp Gln Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Pro Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Val
65 70 75 80
Arg Pro Asp Pro Asp Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg
85 90 95
Glu Ile Cys Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp His Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Gly Pro Gly Ile Cys Thr Val Val Leu Glu Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Ser Thr Ser Ser Ser Ala Pro Pro Pro Pro Ser Glu
195 200 205
<210> 345
<211> 188
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLj，同源于AtPYR1 V81I
<400> 345
Met Glu Pro His Met Glu Ser Ala Leu Arg Gln Gly Leu Ser Glu Ala
1 5 10 15
Glu Gln Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro
20 25 30
Gly Arg Ala Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp
35 40 45
Ala Pro Leu Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro
50 55 60
Gln Arg Tyr Lys His Phe Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp
65 70 75 80
Gly Ala Thr Val Gly Ser Val Arg Glu Ile Thr Val Val Ser Gly Leu
85 90 95
Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg
100 105 110
His Ile Leu Ser Phe Arg Val Val Gly Gly Asp His Arg Leu Arg Asn
115 120 125
Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys
130 135 140
Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu
145 150 155 160
Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln
165 170 175
Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn
180 185
<210> 346
<211> 214
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLd，同源于AtPYR1 V81I
<400> 346
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Ala Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Pro Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Ile Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Phe Val Gly Gly Asp His Arg
130 135 140
Leu Gln Asn Tyr Ser Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly
145 150 155 160
Pro Tyr Cys Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly
165 170 175
Asn Thr Glu Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu
180 185 190
Asn Leu Gln Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln
195 200 205
Thr Glu Pro Leu Asp Arg
210
<210> 347
<211> 213
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLf，同源于AtPYR1 V81I
<400> 347
Glx Met Pro Tyr Met Val Met Val Glu Met Asp Gly Gly Val Gly Gly
1 5 10 15
Gly Gly Gly Gly Gly Gln Thr Pro Ala Pro Arg Arg Trp Arg Leu Ala
20 25 30
Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg Arg
35 40 45
Phe His Arg His Glu Pro Arg Glu His Gln Cys Ser Ser Ala Val Ala
50 55 60
Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg
65 70 75 80
Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu Met
85 90 95
Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Ile Asn Val Lys Ser
100 105 110
Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp
115 120 125
Asn Glu His Ile Leu Ser Val Arg Phe Val Gly Gly Asp His Arg Leu
130 135 140
Gln Asn Tyr Ser Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly Pro
145 150 155 160
Tyr Cys Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn
165 170 175
Thr Glu Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn
180 185 190
Leu Gln Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln Thr
195 200 205
Glu Pro Leu Asp Arg
210
<210> 348
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLe，同源于AtPYR1 V81I
<400> 348
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His
20 25 30
Ala Pro Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Arg Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Ile Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Asn Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Leu Ile Asp Gln
195
<210> 349
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLg，同源于AtPYR1 V81I
<400> 349
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Thr Glu Tyr Val Arg Arg Leu His Arg His
20 25 30
Ala Pro Ala Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Ser Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Ile Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Lys Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Pro Ile Asp Gln
195
<210> 350
<211> 233
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLc，同源于AtPYR1 V81Y
<400> 350
Met Arg Glu Arg Asn Ser Ser Ile Asp Gln Glu His Gln Arg Gly Ser
1 5 10 15
Ser Ser Arg Ser Thr Met Pro Phe Ala Ala Ser Arg Thr Ser Gln Gln
20 25 30
Gln His Ser Arg Val Ala Thr Asn Gly Arg Ala Val Ala Val Cys Ala
35 40 45
Gly His Ala Gly Val Pro Asp Glu Val Ala Arg His His Glu His Ala
50 55 60
Val Ala Ala Gly Gln Cys Cys Ala Ala Met Val Gln Ser Ile Ala Ala
65 70 75 80
Pro Val Asp Ala Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Arg Tyr Lys Arg Phe Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly
100 105 110
Ala Glu Val Gly Ser Val Arg Glu Tyr Leu Leu Val Ser Gly Leu Pro
115 120 125
Ala Glu Ser Ser Arg Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg
130 135 140
Val Ile Ser Phe Arg Val Leu Gly Gly Asp His Arg Leu Ala Asn Tyr
145 150 155 160
Arg Ser Val Thr Thr Val His Glu Ala Ala Pro Ser Gln Asp Gly Arg
165 170 175
Pro Leu Thr Met Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly
180 185 190
Asn Thr Val Glu Glu Thr Arg Ile Phe Val Asp Thr Ile Val Arg Cys
195 200 205
Asn Leu Gln Ser Leu Glu Gly Thr Val Ile Arg Gln Leu Glu Ile Ala
210 215 220
Ala Met Pro His Asp Asp Asn Gln Asn
225 230
<210> 351
<211> 213
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLl，同源于AtPYR1 V81Y
<400> 351
Met Pro Tyr Ala Ala Thr Arg Thr Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Ala Ser Asn Gly Arg Ala Val Ala Ala Cys Ala Gly His Ala Gly Val
20 25 30
Pro Asp Glu Val Ala Arg His His Glu His Ala Val Ala Ala Gly Gln
35 40 45
Cys Cys Ser Val Met Val Gln Ser Ile Ala Ala Pro Ala Asp Ala Val
50 55 60
Trp Ser Leu Val Arg Arg Phe Asp Gln Pro Gln Gly Tyr Lys Arg Phe
65 70 75 80
Ile Arg Ser Cys His Leu Val Asp Gly Asp Gly Val Glu Val Gly Ser
85 90 95
Val Arg Glu Tyr Leu Val Val Ser Gly Leu Pro Ala Glu Asn Ser Arg
100 105 110
Glu Arg Leu Glu Ile Arg Asp Asp Glu Arg Arg Val Ile Ser Phe Arg
115 120 125
Ile Leu Gly Gly Asp His Arg Leu Ala Asn Tyr Arg Ser Val Thr Thr
130 135 140
Val His Glu Ala Ala Ser Glu Gly Gly Pro Leu Thr Met Val Val Glu
145 150 155 160
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Val Glu Glu Thr Arg
165 170 175
Ile Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu Glu Asp
180 185 190
Thr Val Ile Arg Gln Gln Ala Met Ala Ala Pro Ala Ala Pro His Asn
195 200 205
Asp His Asn His Ser
210
<210> 352
<211> 200
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLa，同源于AtPYR1 V81Y
<400> 352
Met Pro Tyr Thr Ala Pro Arg Pro Ser Pro Gln Gln His Ser Arg Val
1 5 10 15
Leu Ser Gly Gly Gly Ala Lys Ala Ala Ser His Gly Ala Ser Cys Ala
20 25 30
Ala Val Pro Ala Glu Val Ala Arg His His Glu His Ala Ala Arg Ala
35 40 45
Gly Gln Cys Cys Ser Ala Val Val Gln Ala Ile Ala Ala Pro Val Gly
50 55 60
Ala Val Trp Ser Val Val Arg Arg Phe Asp Arg Pro Gln Ala Tyr Lys
65 70 75 80
His Phe Ile Arg Ser Cys Arg Leu Val Gly Gly Gly Asp Val Ala Val
85 90 95
Gly Ser Val Arg Glu Tyr Arg Val Val Ser Gly Leu Pro Ala Thr Ser
100 105 110
Ser Arg Glu Arg Leu Glu Ile Leu Asp Asp Glu Arg Arg Val Leu Ser
115 120 125
Phe Arg Val Val Gly Gly Glu His Arg Leu Ala Asn Tyr Arg Ser Val
130 135 140
Thr Thr Val His Glu Ala Gly Ala Gly Ala Gly Thr Gly Thr Val Val
145 150 155 160
Val Glu Ser Tyr Val Val Asp Val Pro His Gly Asn Thr Ala Asp Glu
165 170 175
Thr Arg Val Phe Val Asp Thr Ile Val Arg Cys Asn Leu Gln Ser Leu
180 185 190
Ala Arg Thr Ala Glu Arg Leu Ala
195 200
<210> 353
<211> 220
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLb，同源于AtPYR1 V81Y
<400> 353
Met Pro Cys Ile Gln Ala Ser Ser Pro Gly Gly Met Pro His Gln His
1 5 10 15
Gly Arg Gly Arg Val Leu Gly Gly Gly Val Gly Cys Ala Ala Glu Val
20 25 30
Ala Ala Ala Val Ala Ala Ser Ala Gly Gly Met Arg Cys Gly Ala His
35 40 45
Asp Gly Glu Val Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala
50 55 60
Ala Gly Pro Gly Arg Cys Cys Ser Ala Val Val Gln His Val Ala Ala
65 70 75 80
Pro Ala Ala Ala Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln
85 90 95
Val Tyr Lys Arg Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly
100 105 110
Gly Val Gly Thr Leu Arg Glu Tyr Arg Val Val Ser Gly Leu Pro Ala
115 120 125
Ala Ser Ser Arg Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val
130 135 140
Leu Ser Phe Arg Val Val Gly Gly Glu His Arg Leu Arg Asn Tyr Leu
145 150 155 160
Ser Val Thr Thr Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr
165 170 175
Val Val Val Glu Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro
180 185 190
Glu Asp Thr Arg Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln
195 200 205
Ser Leu Ala Thr Thr Ala Glu Lys Leu Ala Ala Val
210 215 220
<210> 354
<211> 218
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLo，同源于AtPYR1 V81Y
<400> 354
Met Pro Cys Leu Gln Ala Ser Ser Pro Gly Ser Met Pro Tyr Gln His
1 5 10 15
His Gly Arg Gly Val Gly Cys Ala Ala Glu Ala Gly Ala Ala Val Gly
20 25 30
Ala Ser Ala Gly Thr Gly Thr Arg Cys Gly Ala His Asp Gly Glu Val
35 40 45
Pro Ala Glu Ala Ala Arg His His Glu His Ala Ala Pro Gly Pro Gly
50 55 60
Arg Cys Cys Ser Ala Val Val Gln Arg Val Ala Ala Pro Ala Glu Ala
65 70 75 80
Val Trp Ser Val Val Arg Arg Phe Asp Gln Pro Gln Ala Tyr Lys Arg
85 90 95
Phe Val Arg Ser Cys Ala Leu Leu Ala Gly Asp Gly Gly Val Gly Thr
100 105 110
Leu Arg Glu Tyr Arg Val Val Ser Gly Leu Pro Ala Ala Ser Ser Arg
115 120 125
Glu Arg Leu Glu Val Leu Asp Asp Glu Ser His Val Leu Ser Phe Arg
130 135 140
Val Val Gly Gly Glu His Arg Leu Gln Asn Tyr Leu Ser Val Thr Thr
145 150 155 160
Val His Pro Ser Pro Ala Ala Pro Asp Ala Ala Thr Val Val Val Glu
165 170 175
Ser Tyr Val Val Asp Val Pro Pro Gly Asn Thr Pro Glu Asp Thr Arg
180 185 190
Val Phe Val Asp Thr Ile Val Lys Cys Asn Leu Gln Ser Leu Ala Thr
195 200 205
Thr Ala Glu Lys Leu Ala Leu Ala Ala Val
210 215
<210> 355
<211> 212
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLi，同源于AtPYR1 V81Y
<400> 355
Met Asp Gln Gln Gly Ala Gly Gly Asp Ala Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Ser Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Glu Ala Val Trp Ala Val Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Leu
65 70 75 80
Arg Pro Asp Pro Glu Ala Gly Asp Ala Leu Cys Pro Gly Arg Leu Arg
85 90 95
Glu Tyr Ser Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp Leu Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Asp Pro Ala Ile Cys Thr Val Val Leu Glu Ser Tyr Val
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Asn Ala Ala Glu Ala Ala Ala Thr Thr Asn Ser Val Leu Leu Pro
195 200 205
Arg Pro Ala Glu
210
<210> 356
<211> 205
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLk，同源于AtPYR1 V81Y
<400> 356
Met Asp Gln Gln Gly Ala Gly Gly Asp Val Glu Val Pro Ala Gly Leu
1 5 10 15
Gly Leu Thr Ala Ala Glu Tyr Glu Gln Leu Arg Pro Thr Val Asp Ala
20 25 30
His His Arg Tyr Ala Val Gly Glu Gly Gln Cys Ser Ser Leu Leu Ala
35 40 45
Gln Arg Ile His Ala Pro Pro Ala Ala Val Trp Ala Ile Val Arg Arg
50 55 60
Phe Asp Cys Pro Gln Val Tyr Lys His Phe Ile Arg Ser Cys Ala Val
65 70 75 80
Arg Pro Asp Pro Asp Ala Gly Asp Ala Leu Arg Pro Gly Arg Leu Arg
85 90 95
Glu Tyr Cys Val Ile Ser Gly Leu Pro Ala Ser Thr Ser Thr Glu Arg
100 105 110
Leu Asp His Leu Asp Asp Ala Ala Arg Val Phe Gly Phe Ser Ile Thr
115 120 125
Gly Gly Glu His Arg Leu Arg Asn Tyr Arg Ser Val Thr Thr Val Ser
130 135 140
Glu Leu Ala Gly Pro Gly Ile Cys Thr Val Val Leu Glu Ser Tyr Ala
145 150 155 160
Val Asp Val Pro Asp Gly Asn Thr Glu Asp Asp Thr Arg Leu Phe Ala
165 170 175
Asp Thr Val Ile Arg Leu Asn Leu Gln Lys Leu Lys Ser Val Ala Glu
180 185 190
Ala Ser Thr Ser Ser Ser Ala Pro Pro Pro Pro Ser Glu
195 200 205
<210> 357
<211> 188
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLj，同源于AtPYR1 V81Y
<400> 357
Met Glu Pro His Met Glu Ser Ala Leu Arg Gln Gly Leu Ser Glu Ala
1 5 10 15
Glu Gln Arg Glu Leu Glu Gly Val Val Arg Ala His His Thr Phe Pro
20 25 30
Gly Arg Ala Pro Gly Thr Cys Thr Ser Leu Val Thr Gln Arg Val Asp
35 40 45
Ala Pro Leu Ala Ala Val Trp Pro Ile Val Arg Gly Phe Gly Ser Pro
50 55 60
Gln Arg Tyr Lys His Phe Ile Lys Ser Cys Asp Leu Lys Ala Gly Asp
65 70 75 80
Gly Ala Thr Val Gly Ser Val Arg Glu Tyr Thr Val Val Ser Gly Leu
85 90 95
Pro Ala Ser Thr Ser Thr Glu Arg Leu Glu Ile Leu Asp Asp His Arg
100 105 110
His Ile Leu Ser Phe Arg Val Val Gly Gly Asp His Arg Leu Arg Asn
115 120 125
Tyr Arg Ser Val Thr Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys
130 135 140
Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu
145 150 155 160
Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln
165 170 175
Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn
180 185
<210> 358
<211> 214
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLd，同源于AtPYR1 V81Y
<400> 358
Met Val Val Glu Met Asp Gly Gly Val Gly Val Ala Ala Ala Gly Gly
1 5 10 15
Gly Gly Ala Gln Thr Pro Ala Pro Pro Pro Pro Arg Arg Trp Arg Leu
20 25 30
Ala Asp Glu Arg Cys Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg
35 40 45
Arg Phe His Arg His Glu Pro Arg Asp His Gln Cys Ser Ser Ala Val
50 55 60
Ala Lys His Ile Lys Ala Pro Val His Leu Val Trp Ser Leu Val Arg
65 70 75 80
Arg Phe Asp Gln Pro Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu
85 90 95
Met Lys Gly Asn Ile Glu Ile Gly Ser Val Arg Glu Tyr Asn Val Lys
100 105 110
Ser Gly Leu Pro Ala Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp
115 120 125
Asp Asp Glu Arg Ile Leu Ser Val Arg Phe Val Gly Gly Asp His Arg
130 135 140
Leu Gln Asn Tyr Ser Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly
145 150 155 160
Pro Tyr Cys Val Val Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly
165 170 175
Asn Thr Glu Glu Asp Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu
180 185 190
Asn Leu Gln Lys Leu Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln
195 200 205
Thr Glu Pro Leu Asp Arg
210
<210> 359
<211> 209
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLf，同源于AtPYR1 V81Y
<400> 359
Met Val Met Val Glu Met Asp Gly Gly Val Gly Gly Gly Gly Gly Gly
1 5 10 15
Gly Gln Thr Pro Ala Pro Arg Arg Trp Arg Leu Ala Asp Glu Arg Cys
20 25 30
Asp Leu Arg Ala Met Glu Thr Asp Tyr Val Arg Arg Phe His Arg His
35 40 45
Glu Pro Arg Glu His Gln Cys Ser Ser Ala Val Ala Lys His Ile Lys
50 55 60
Ala Pro Val His Leu Val Trp Ser Leu Val Arg Arg Phe Asp Gln Pro
65 70 75 80
Gln Leu Phe Lys Pro Phe Val Ser Arg Cys Glu Met Lys Gly Asn Ile
85 90 95
Glu Ile Gly Ser Val Arg Glu Tyr Asn Val Lys Ser Gly Leu Pro Ala
100 105 110
Thr Arg Ser Thr Glu Arg Leu Glu Leu Leu Asp Asp Asn Glu His Ile
115 120 125
Leu Ser Val Arg Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr Ser
130 135 140
Ser Ile Leu Ser Val Thr Glu Phe Gln Pro Gly Pro Tyr Cys Val Val
145 150 155 160
Leu Glu Ser Tyr Val Val Asp Val Pro Asp Gly Asn Thr Glu Glu Asp
165 170 175
Thr Arg Met Phe Thr Asp Thr Val Val Lys Leu Asn Leu Gln Lys Leu
180 185 190
Ala Ala Ile Ala Thr Ser Ser Ser Ala Asn Gln Thr Glu Pro Leu Asp
195 200 205
Arg
<210> 360
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLe，同源于AtPYR1 V81Y
<400> 360
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Ala Glu Tyr Val Arg Arg Met His Arg His
20 25 30
Ala Pro Thr Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Arg Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Tyr Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Asn Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Leu Ile Asp Gln
195
<210> 361
<211> 197
<212> PRT
<213> 人工序列
<220>
<223> 合成ZmPYLg，同源于AtPYR1 V81Y
<400> 361
Met Val Gly Leu Val Gly Gly Ser Thr Ala Arg Ala Glu His Val Val
1 5 10 15
Ala Asn Ala Gly Gly Glu Thr Glu Tyr Val Arg Arg Leu His Arg His
20 25 30
Ala Pro Ala Glu His Gln Cys Thr Ser Thr Leu Val Lys His Ile Lys
35 40 45
Ala Pro Val His Leu Val Trp Glu Leu Val Arg Ser Phe Asp Gln Pro
50 55 60
Gln Arg Tyr Lys Pro Phe Val Arg Asn Cys Val Val Arg Gly Asp Gln
65 70 75 80
Leu Glu Val Gly Ser Leu Arg Asp Tyr Asn Val Lys Thr Gly Leu Pro
85 90 95
Ala Thr Thr Ser Thr Glu Arg Leu Glu Gln Leu Asp Asp Asp Leu His
100 105 110
Ile Leu Gly Val Lys Phe Val Gly Gly Asp His Arg Leu Gln Asn Tyr
115 120 125
Ser Ser Ile Ile Thr Val His Pro Glu Ser Ile Asp Gly Arg Pro Gly
130 135 140
Thr Leu Val Ile Glu Ser Phe Val Val Asp Val Pro Asp Gly Asn Thr
145 150 155 160
Lys Asp Glu Thr Cys Tyr Phe Val Glu Ala Val Ile Lys Cys Asn Leu
165 170 175
Lys Ser Leu Ala Glu Val Ser Glu Gln Leu Ala Val Glu Ser Pro Thr
180 185 190
Ser Pro Ile Asp Gln
195
<210> 362
<400> 362
000
<210> 363
<400> 363
000
<210> 364
<400> 364
000
<210> 365
<400> 365
000
<210> 366
<400> 366
000
<210> 367
<400> 367
000
<210> 368
<400> 368
000
<210> 369
<400> 369
000
<210> 370
<400> 370
000
<210> 371
<400> 371
000
<210> 372
<400> 372
000
<210> 373
<400> 373
000
<210> 374
<400> 374
000
<210> 375
<400> 375
000
<210> 376
<400> 376
000
<210> 377
<400> 377
000
<210> 378
<400> 378
000
<210> 379
<400> 379
000
<210> 380
<400> 380
000
<210> 381
<400> 381
000
<210> 382
<400> 382
000
<210> 383
<400> 383
000
<210> 384
<400> 384
000
<210> 385
<400> 385
000
<210> 386
<400> 386
000
<210> 387
<400> 387
000
<210> 388
<400> 388
000
<210> 389
<400> 389
000
<210> 390
<400> 390
000
<210> 391
<400> 391
000
<210> 392
<400> 392
000
<210> 393
<400> 393
000
<210> 394
<400> 394
000
<210> 395
<400> 395
000
<210> 396
<400> 396
000
<210> 397
<400> 397
000
<210> 398
<400> 398
000
<210> 399
<400> 399
000
<210> 400
<400> 400
000
<210> 401
<400> 401
000
<210> 402
<400> 402
000
<210> 403
<400> 403
000
<210> 404
<400> 404
000
<210> 405
<400> 405
000
<210> 406
<400> 406
000
<210> 407
<400> 407
000
<210> 408
<400> 408
000
<210> 409
<400> 409
000
<210> 410
<400> 410
000
<210> 411
<211> 23
<212> PRT
<213> 人工序列
<220>
<223> 合成肽，包含接头和烟草
蚀纹病毒(TEV)蛋白酶切割位点
<400> 411
Gly Gly Gly Ser Gln Phe Gly Ser Gly Gly Gly Gly Gly Ser Gly Ser
1 5 10 15
Glu Asn Leu Tyr Phe Gln Ser
20

Claims (33)

1.一种分离的核酸，其包含编码突变的PYR/PYL受体多肽的多核苷酸，所述突变的PYR/PYL受体多肽包含应于SEQ ID NO:1所示的PYR1中的氨基酸F61、V81、I110、E141和A160的氨基酸取代，其中，所述突变的PYR/PYL受体相较于缺乏所述取代的对照PYR/PYL受体对于脱落酸具有增加的敏感性。
2.如权利要求1所述的分离的核酸，其中，所述PYR/PYL受体多肽包含对应于氨基酸F61的氨基酸取代。
3.如权利要求2所述的分离的核酸，其中，所述氨基酸取代选自L和M。
4.如权利要求1所述的分离的核酸，其中，所述PYR/PYL受体多肽包含对应于氨基酸V81的氨基酸取代。
5.如权利要求4所述的分离的核酸，其中，所述氨基酸取代选自I和Y。
6.如权利要求1所述的分离的核酸，其中，所述PYR/PYL受体多肽包含对应于氨基酸I110的氨基酸取代。
7.如权利要求6所述的分离的核酸，其中，所述氨基酸取代选自C和S。
8.如权利要求1所述的分离的核酸，其中，所述PYR/PYL受体多肽包含对应于氨基酸E141的氨基酸取代。
9.如权利要求8所述的分离的核酸，其中，所述氨基酸取代选自C、I、L、M、N、T、V、W和Y。
10.如权利要求1所述的分离的核酸，其中，所述PYR/PYL受体多肽包含对应于氨基酸A160的氨基酸取代。
11.如权利要求8所述的分离的核酸，其中，所述氨基酸取代选自C、I和V。
12.如权利要求1-11中任一项所述的分离的核酸，其中，所述突变的PYR/PYL受体多肽基本相同于SEQ ID NO:1-119或SEQ ID NO:155-361中的任一项，或包含SEQ ID NO:120-123中的任一项。
13.如权利要求1-12任一项所述的分离的核酸，其中，所述多核苷酸编码融合蛋白，所述融合蛋白包含所述突变的PYR/PYL受体多肽和融合伴侣蛋白。
14.如权利要求13所述的分离的核酸，其中，所述融合伴侣蛋白是转录活化或调节结构域。
15.如权利要求14所述的分离的核酸，其中，所述转录活化物是VP16或VP64。
16.如权利要求13-15中任一项所述的分离的核酸，其中，所述融合蛋白还包含核定位信号序列。
17.一种细胞，其含有异源性的如权利要求1-16中任一项所述的多核苷酸。
18.如权利要求17所述的细胞，其中，所述细胞是非植物真核细胞。
19.一种植物，其包含如权利要求1-16中任一项所述的多核苷酸。
20.一种植物，其包含原位突变的PYR/PYL受体多肽，所述突变的PYR/PYL受体多肽包含对应于SEQ ID NO:1所示的PYR1中的氨基酸F61、V81、I110、E141和A160的氨基酸取代，其中，相较于缺乏所述取代的对照PYR/PYL受体，所述突变的PYR/PYL受体多肽对于脱落酸具有增加的敏感性。
21.一种表达盒，其包含操作性地连接至如权利要求1-16中任一项所述的多核苷酸的启动子，其中，将所述表达盒引入植物将导致，所述植物相较于缺乏所述表达盒的对照植物对于脱落酸具有增加的敏感性。
22.如权利要求21所述的表达盒，其中，所述启动子对于所述多核苷酸是异源性的。
23.如权利要求21所述的表达盒，其中，所述启动子是诱导型的。
24.如权利要求21所述的表达盒，其中，所述启动子是胁迫诱导型启动子。
25.一种表达载体，其包含如权利要求21-24中任一项所述的表达盒。
26.一种植物，其包含如权利要求21-24中任一项所述的表达盒，其中，所述植物相较于缺乏所述表达盒的对照植物对于脱落酸具有增加的敏感性。
27.来自如权利要求26或19所述的植物的植物细胞。
28.来自如权利要求26或19所述的植物的种子、花、叶、果、经加工食物或食物成分。
29.一种产生对于脱落酸具有增加的敏感性的植物的方法，所述方法包括：
将如权利要求21-24中任一项所述的表达盒引入多个植物；和
从所述多个植物中选择表达所述多核苷酸的植物。
30.一种产生对于脱落酸具有增加的敏感性的植物的方法，所述方法包括：
将突变引入编码PYR/PYL多肽的多核苷酸，其中，所述突变导致如权利要求1-16中任一项所述的多核苷酸。
31.如权利要求30所述的方法，其中，所述引入在植物细胞的基因组中原位发生。
32.如权利要求31所述的方法，其中，所述引入包括规律成簇的间隔短回文重复(CRISPR)/Cas基因组编辑。
33.一种导向核糖核酸(gRNA)，其包含：
a)CRISPR核糖核酸(crRNA)，其基本相同于SEQ ID NO:363、364、365、366、367或369；和
b)反式作用核糖核酸(tracRNA)，
其中，所述PYR/PYL突变靶位点包含编码PYL-E的V89或PYL-E的E149的核酸。
CN201580058071.XA 2014-08-26 2015-08-26 超敏aba受体 Pending CN107207573A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201462042095P 2014-08-26 2014-08-26
US62/042,095 2014-08-26
US201462098025P 2014-12-30 2014-12-30
US62/098,025 2014-12-30
PCT/US2015/047020 WO2016033230A1 (en) 2014-08-26 2015-08-26 Hypersensitive aba receptors

Publications (1)

Publication Number Publication Date
CN107207573A true CN107207573A (zh) 2017-09-26

Family

ID=54073002

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201580058071.XA Pending CN107207573A (zh) 2014-08-26 2015-08-26 超敏aba受体

Country Status (6)

Country Link
US (2) US20160194653A1 (zh)
EP (1) EP3194427A1 (zh)
CN (1) CN107207573A (zh)
BR (1) BR112017003528A2 (zh)
CA (1) CA2958767A1 (zh)
WO (1) WO2016033230A1 (zh)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019063009A1 (zh) * 2017-09-30 2019-04-04 中国科学院上海生命科学研究院 一种提高水稻产量的aba受体pyl家族基因组合敲除的方法及其应用
CN111733181A (zh) * 2020-04-24 2020-10-02 兰州大学 一种生物安全型abcg11基因超表达载体的构建方法
CN112759631A (zh) * 2021-01-21 2021-05-07 深圳市儿童医院 Pyl3蛋白突变体及其应用

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3613852A3 (en) 2011-07-22 2020-04-22 President and Fellows of Harvard College Evaluation and improvement of nuclease cleavage specificity
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US9068179B1 (en) 2013-12-12 2015-06-30 President And Fellows Of Harvard College Methods for correcting presenilin point mutations
AU2015298571B2 (en) 2014-07-30 2020-09-03 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
CA2958767A1 (en) 2014-08-26 2016-03-03 The Regents Of The University Of California Hypersensitive aba receptors
IL241462A0 (en) 2015-09-10 2015-11-30 Yeda Res & Dev Heterologous engineering of betalain pigments in plants
CN108513575A (zh) 2015-10-23 2018-09-07 哈佛大学的校长及成员们 核碱基编辑器及其用途
WO2018027078A1 (en) 2016-08-03 2018-02-08 President And Fellows Of Harard College Adenosine nucleobase editors and uses thereof
CA3033327A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
IL247752A0 (en) * 2016-09-11 2016-11-30 Yeda Res & Dev Compositions and methods for modulating gene expression for site-directed mutagenesis
KR20240007715A (ko) 2016-10-14 2024-01-16 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
WO2018140899A1 (en) 2017-01-28 2018-08-02 Inari Agriculture, Inc. Novel plant cells, plants, and seeds
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
JP2020510439A (ja) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ シトシンからグアニンへの塩基編集因子
SG11201908658TA (en) 2017-03-23 2019-10-30 Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
US11732274B2 (en) 2017-07-28 2023-08-22 President And Fellows Of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE)
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
CN111757937A (zh) 2017-10-16 2020-10-09 布罗德研究所股份有限公司 腺苷碱基编辑器的用途
US11220694B1 (en) 2018-01-29 2022-01-11 Inari Agriculture, Inc. Rice cells and rice plants
US11802288B1 (en) 2018-01-29 2023-10-31 Inari Agriculture Technology, Inc. Methods for efficient soybean genome editing
US11926835B1 (en) 2018-01-29 2024-03-12 Inari Agriculture Technology, Inc. Methods for efficient tomato genome editing
US11866719B1 (en) 2018-06-04 2024-01-09 Inari Agriculture Technology, Inc. Heterologous integration of regulatory elements to alter gene expression in wheat cells and wheat plants
DE112020001342T5 (de) 2019-03-19 2022-01-13 President and Fellows of Harvard College Verfahren und Zusammensetzungen zum Editing von Nukleotidsequenzen
EP4146804A1 (en) 2020-05-08 2023-03-15 The Broad Institute Inc. Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
WO2023141591A2 (en) * 2022-01-20 2023-07-27 The Regents Of The University Of California Reagents and systems for generating biosensors

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110271408A1 (en) * 2010-04-28 2011-11-03 Regents Of The University Of California Modified pyr/pyl receptors activated by orthogonal ligands
WO2013006263A2 (en) * 2011-07-01 2013-01-10 The Regents Of The University Of California Constitutively active aba receptor mutants

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106434738A (zh) * 2009-02-13 2017-02-22 加州大学董事会 用于改善植物抗逆性的组成型活性的pyr/pyl受体蛋白
CA2958767A1 (en) 2014-08-26 2016-03-03 The Regents Of The University Of California Hypersensitive aba receptors

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110271408A1 (en) * 2010-04-28 2011-11-03 Regents Of The University Of California Modified pyr/pyl receptors activated by orthogonal ligands
WO2013006263A2 (en) * 2011-07-01 2013-01-10 The Regents Of The University Of California Constitutively active aba receptor mutants

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SANDER JD等: "CRISPR-Cas systems for editing, regulating and targeting genomes", 《NATURE BIOTECHNOLOGY》 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019063009A1 (zh) * 2017-09-30 2019-04-04 中国科学院上海生命科学研究院 一种提高水稻产量的aba受体pyl家族基因组合敲除的方法及其应用
CN109593775A (zh) * 2017-09-30 2019-04-09 中国科学院上海生命科学研究院 一种提高水稻产量的aba受体pyl家族基因组合敲除的方法及其应用
US11603537B2 (en) 2017-09-30 2023-03-14 Cas Center For Excellence In Molecular Plant Sciences Method for improving rice yield by jointly knocking out ABA receptor PYL family genes and use thereof
CN111733181A (zh) * 2020-04-24 2020-10-02 兰州大学 一种生物安全型abcg11基因超表达载体的构建方法
CN112759631A (zh) * 2021-01-21 2021-05-07 深圳市儿童医院 Pyl3蛋白突变体及其应用

Also Published As

Publication number Publication date
US10934559B2 (en) 2021-03-02
WO2016033230A1 (en) 2016-03-03
CA2958767A1 (en) 2016-03-03
BR112017003528A2 (pt) 2018-07-10
US20160194653A1 (en) 2016-07-07
EP3194427A1 (en) 2017-07-26
US20180265886A1 (en) 2018-09-20

Similar Documents

Publication Publication Date Title
CN107207573A (zh) 超敏aba受体
CN101855355B (zh) 具有提高的产量相关性状的植物和用于制备该植物的方法
CN101868544B (zh) 具有提高的产量相关性状的植物和用于制备该植物的方法
EP2563113B1 (en) Modified pyr/pyl receptors activated by orthogonal ligands
CN108064129A (zh) 玉米和大豆中复合性状基因座的位点特异性整合位点的产生和使用方法
CN108603197B (zh) 提高植物氮利用效率的方法
US10913954B2 (en) Abiotic stress tolerant plants and methods
CN115927380A (zh) 具有增强性状的转基因植物
CN111511198A (zh) 具有增强的性状的经修饰的植物
CN104293802B (zh) 百脉根erf类转录因子、其编码基因及表达载体和应用
WO2015007241A1 (en) Molecular marker
EP2970996B1 (en) Modified pyr/pyl receptors activated by orthogonal ligands
US20170183680A1 (en) Dominant negative mutant krp-related proteins (krp) in zea mays and methods of their use
WO2023183895A2 (en) Use of cct-domain proteins to improve agronomic traits of plants
Nejat Gene editing of the representative WRKY family members in an elite malting barley cultivar RGT Planet by CRISPR/Cas9
US20220145319A1 (en) Engineered phosphoenolpyruvate carboxylase enzymes
US20180127767A1 (en) Induction of latex accumulation in rubber-producing shrubs
US11414673B2 (en) Hypersensitive ABA receptors having modified PP2C-binding interfaces
CN113773375B (zh) 大豆核因子蛋白GmNF307在植物耐盐调控中的应用
Weiguo et al. Applicatios of Genetic Engineering in Mulberry
WO2013072914A2 (en) Plant rad52 and uses thereof
WO2023067574A1 (en) Compositions and methods comprising plants with modified sugar content
Bansal et al. Landmarks of Biotechnology in Agriculture: An over-view

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20170926