CN107207551B - 使用具有转半乳糖基化活性的酶生产含半乳糖和果糖部分的糖类的方法 - Google Patents

使用具有转半乳糖基化活性的酶生产含半乳糖和果糖部分的糖类的方法 Download PDF

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Publication number
CN107207551B
CN107207551B CN201580072304.1A CN201580072304A CN107207551B CN 107207551 B CN107207551 B CN 107207551B CN 201580072304 A CN201580072304 A CN 201580072304A CN 107207551 B CN107207551 B CN 107207551B
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China
Prior art keywords
polypeptide
saccharide
seq
less
fructose
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Expired - Fee Related
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CN201580072304.1A
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English (en)
Chinese (zh)
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CN107207551A (zh
Inventor
M·K·拉尔森
J·F·克拉米尔
T·艾泽勒
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International N&H Denmark ApS
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DuPont Nutrition Biosciences ApS
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Priority claimed from GBGB1419894.9A external-priority patent/GB201419894D0/en
Priority claimed from GBGB1515645.8A external-priority patent/GB201515645D0/en
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Publication of CN107207551A publication Critical patent/CN107207551A/zh
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/1203—Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
    • A23C9/1206—Lactose hydrolysing enzymes, e.g. lactase, beta-galactosidase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/20—Dietetic milk products not covered by groups A23C9/12 - A23C9/18
    • A23C9/203—Dietetic milk products not covered by groups A23C9/12 - A23C9/18 containing bifidus-active substances, e.g. lactulose; containing oligosaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/04—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C13—SUGAR INDUSTRY
    • C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
    • C13K13/00—Sugars not otherwise provided for in this class
    • C13K13/005—Lactulose
    • C—CHEMISTRY; METALLURGY
    • C13—SUGAR INDUSTRY
    • C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
    • C13K13/00—Sugars not otherwise provided for in this class

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Dairy Products (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Saccharide Compounds (AREA)
CN201580072304.1A 2014-11-07 2015-11-06 使用具有转半乳糖基化活性的酶生产含半乳糖和果糖部分的糖类的方法 Expired - Fee Related CN107207551B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB1419894.9A GB201419894D0 (en) 2014-11-07 2014-11-07 Method
GB1419894.9 2014-11-07
GBGB1515645.8A GB201515645D0 (en) 2015-09-03 2015-09-03 Method
GB1515645.8 2015-09-03
PCT/EP2015/075948 WO2016071502A1 (en) 2014-11-07 2015-11-06 Method of generating a saccharide containing a galactose and a fructose moiety employing enzyme with transgalactosylating activity

Publications (2)

Publication Number Publication Date
CN107207551A CN107207551A (zh) 2017-09-26
CN107207551B true CN107207551B (zh) 2021-03-12

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CN201580072304.1A Expired - Fee Related CN107207551B (zh) 2014-11-07 2015-11-06 使用具有转半乳糖基化活性的酶生产含半乳糖和果糖部分的糖类的方法

Country Status (8)

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US (2) US20170339970A1 (https=)
EP (1) EP3215628A1 (https=)
JP (1) JP6851967B2 (https=)
CN (1) CN107207551B (https=)
AU (2) AU2015341682A1 (https=)
BR (1) BR112017008862A2 (https=)
MX (1) MX2017005770A (https=)
WO (1) WO2016071502A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220325307A1 (en) * 2018-12-03 2022-10-13 Dupont Nutrition Biosciences Aps Use of lactase to provide high gos fiber level and low lactose level
CN116535448B (zh) * 2023-05-26 2024-09-06 中国海洋大学 一种新型乳果糖及其制备方法和应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1614357A1 (en) * 2004-07-10 2006-01-11 Cognis IP Management GmbH Dietary supplements comprising prebiotics and fatty acid
CN102168028A (zh) * 2010-02-26 2011-08-31 江南大学 一种节杆菌突变株、利用该突变株生产乳糖酶的方法及用乳糖酶制备乳果糖的方法
CN103080329A (zh) * 2010-07-19 2013-05-01 阿尔拉食品公司 含有半乳寡聚糖的组合物及其生产方法
WO2013182686A1 (en) * 2012-06-08 2013-12-12 Dupont Nutrition Biosciences Aps Polypeptides having transgalactosylating activity

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4940957B1 (https=) * 1970-03-23 1974-11-06
JPH08168393A (ja) * 1994-12-16 1996-07-02 Dainippon Ink & Chem Inc ガラクトオリゴ糖の製造方法
KR100878968B1 (ko) * 2000-05-26 2009-01-19 아를라 푸즈 에이엠비에이 비피도박테륨에서 단리한 베타-갈락토시다제
KR101363293B1 (ko) * 2012-03-06 2014-02-19 고려대학교 산학협력단 락툴로오스 합성용 마이크로 반응기 및 락툴로오스 생산 방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1614357A1 (en) * 2004-07-10 2006-01-11 Cognis IP Management GmbH Dietary supplements comprising prebiotics and fatty acid
CN102168028A (zh) * 2010-02-26 2011-08-31 江南大学 一种节杆菌突变株、利用该突变株生产乳糖酶的方法及用乳糖酶制备乳果糖的方法
CN103080329A (zh) * 2010-07-19 2013-05-01 阿尔拉食品公司 含有半乳寡聚糖的组合物及其生产方法
WO2013182686A1 (en) * 2012-06-08 2013-12-12 Dupont Nutrition Biosciences Aps Polypeptides having transgalactosylating activity

Also Published As

Publication number Publication date
WO2016071502A1 (en) 2016-05-12
US20170339970A1 (en) 2017-11-30
CN107207551A (zh) 2017-09-26
AU2020203922A1 (en) 2020-07-02
JP6851967B2 (ja) 2021-03-31
AU2015341682A1 (en) 2017-05-11
US20200296980A1 (en) 2020-09-24
MX2017005770A (es) 2017-07-28
BR112017008862A2 (pt) 2018-01-16
JP2017533713A (ja) 2017-11-16
EP3215628A1 (en) 2017-09-13

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