CN107201406B - A kind of arborescent ceratoids specificity microsatellite molecular marker and its application - Google Patents
A kind of arborescent ceratoids specificity microsatellite molecular marker and its application Download PDFInfo
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Abstract
Present invention firstly discloses 22 specific microsatellite sequences of arborescent ceratoids and 22 pairs of specific micro-satellite primers.These sites play an important role in analysis of genetic diversity, conservative genetics research, Germplasm Resource Investigation and the assistant breeding to arborescent ceratoids, also provide basic material to study the research of plant drought mechanism.
Description
Technical field
The invention belongs to field of molecular marker, and in particular to a kind of arborescent ceratoids specificity microsatellite molecular marker and its
Using.
Background technique
Arborescent ceratoids (Ceratoides arborescens) are subordinate to Chenopodiaceae ceratoides, are Chinese endemic plant,
It is full of nutrition, palatability is good, be arid, the excellent forage grass in semiarid zone and with characteristics such as drought resisting, cold-resistant, impoverishment tolerants,
And it water and soil conservation, checks winds and fixes drifting sand, the important herbage of land management.Therefore, arborescent ceratoids in Pasture improvement, prevent grassland
Have great importance in " three change " and the upgrading synergy application of desert steppe.Basic scientific research to arborescent ceratoids is pair
It, which carries out resource management, population reconstruction and utilization of resources etc., great importance, but focuses mostly at present to the direction that it is studied
In Physiology and biochemistry etc., still lacks and carry out the germ plasm resources genetic background tune such as genetic diversity and Genetic Constitution of Population to it
It looks into, germ plasm resource genetic background investigation result further instructs formulation and assistant breeding of protection scheme etc..And understand North China
The germ plasm resource genetic background of ceratoides latens, primary work are the molecular labelings for the efficient specificity of its exploitation.
Microsatellite (Microsatellite) is also referred to as SSR (Simple Sequence Repeats), is by 1~6 core
Thuja acid be repeat unit group at the tandem repetitive sequence up to tens nucleotide, be distributed widely in eukaryotic gene group
In.Since it has, the PCR amplification that quantity is more, special, stability are good, be evenly distributed in genome, polymorphism information is abundant, easily
Become one of widely used molecular labeling in recent years in many advantages, such as characteristics such as detection.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of arborescent ceratoids specificity microsatellite locus, including T1,
T6、T11、T13、T15、T16、T17、T20、T22、T23、T25、T26、T28、T29、T30、T31、T34、T35、T36、T37、
The one or more of T38 and T39 nucleotide sequence, nucleotide sequence sequence as shown in SEQIDNO1~SEQIDNO22.
It is described special the present invention also provides for identifying the specific primer pair of above-mentioned arborescent ceratoids microsatellite locus
The nucleotide sequence of property primer pair is respectively as follows:
T1 primer pair sequence is as shown in SEQ ID NO.23 and SEQ ID NO.24；
T6 primer pair sequence is as shown in SEQ ID NO.25 and SEQ ID NO.26；
T11 primer pair sequence is as shown in SEQ ID NO.27 and SEQ ID NO.28；
T13 primer pair sequence is as shown in SEQ ID NO.29 and SEQ ID NO.30；
T15 primer pair sequence is as shown in SEQ ID NO.31 and SEQ ID NO.32；
T16 primer pair sequence is as shown in SEQ ID NO.33 and SEQ ID NO.34；
T17 primer pair sequence is as shown in SEQ ID NO.35 and SEQ ID NO.36；
T20 primer pair sequence is as shown in SEQ ID NO.37 and SEQ ID NO.38；
T22 primer pair sequence is as shown in SEQ ID NO.39 and SEQ ID NO.40；
T23 primer pair sequence is as shown in SEQ ID NO.41 and SEQ ID NO.42；
T25 primer pair sequence is as shown in SEQ ID NO.43 and SEQ ID NO.44；
T26 primer pair sequence is as shown in SEQ ID NO.45 and SEQ ID NO.46；
T28 primer pair sequence is as shown in SEQ ID NO.47 and SEQ ID NO.48；
T29 primer pair sequence is as shown in SEQ ID NO.49 and SEQ ID NO.50；
T30 primer pair sequence is as shown in SEQ ID NO.51 and SEQ ID NO.52；
T31 primer pair sequence is as shown in SEQ ID NO.53 and SEQ ID NO.54；
T34 primer pair sequence is as shown in SEQ ID NO.55 and SEQ ID NO.56；
T35 primer pair sequence is as shown in SEQ ID NO.57 and SEQ ID NO.58；
T36 primer pair sequence is as shown in SEQ ID NO.59 and SEQ ID NO.60；
T37 primer pair sequence is as shown in SEQ ID NO.61 and SEQ ID NO.62；
T38 primer pair sequence is as shown in SEQ ID NO.63 and SEQ ID NO.64；
T39 primer pair sequence is as shown in SEQ ID NO.65 and SEQ ID NO.66.
Another object of the present invention is to provide for identifying the kit of arborescent ceratoids microsatellite locus, including DNA
Polymerase, dNTPs, reaction buffer, primer pair and DNA profiling, wherein the primer pair is one kind or several of following primer pair
Kind:
T1 primer pair sequence is as shown in SEQ ID NO.23 and SEQ ID NO.24；
T6 primer pair sequence is as shown in SEQ ID NO.25 and SEQ ID NO.26；
T11 primer pair sequence is as shown in SEQ ID NO.27 and SEQ ID NO.28；
T13 primer pair sequence is as shown in SEQ ID NO.29 and SEQ ID NO.30；
T15 primer pair sequence is as shown in SEQ ID NO.31 and SEQ ID NO.32；
T16 primer pair sequence is as shown in SEQ ID NO.33 and SEQ ID NO.34；
T17 primer pair sequence is as shown in SEQ ID NO.35 and SEQ ID NO.36；
T20 primer pair sequence is as shown in SEQ ID NO.37 and SEQ ID NO.38；
T22 primer pair sequence is as shown in SEQ ID NO.39 and SEQ ID NO.40；
T23 primer pair sequence is as shown in SEQ ID NO.41 and SEQ ID NO.42；
T25 primer pair sequence is as shown in SEQ ID NO.43 and SEQ ID NO.44；
T26 primer pair sequence is as shown in SEQ ID NO.45 and SEQ ID NO.46；
T28 primer pair sequence is as shown in SEQ ID NO.47 and SEQ ID NO.48；
T29 primer pair sequence is as shown in SEQ ID NO.49 and SEQ ID NO.50；
T30 primer pair sequence is as shown in SEQ ID NO.51 and SEQ ID NO.52；
T31 primer pair sequence is as shown in SEQ ID NO.53 and SEQ ID NO.54；
T34 primer pair sequence is as shown in SEQ ID NO.55 and SEQ ID NO.56；
T35 primer pair sequence is as shown in SEQ ID NO.57 and SEQ ID NO.58；
T36 primer pair sequence is as shown in SEQ ID NO.59 and SEQ ID NO.60；
T37 primer pair sequence is as shown in SEQ ID NO.61 and SEQ ID NO.62；
T38 primer pair sequence is as shown in SEQ ID NO.63 and SEQ ID NO.64；
T39 primer pair sequence is as shown in SEQ ID NO.65 and SEQ ID NO.66.
Preferably, the present invention is used to identify the kit of arborescent ceratoids microsatellite locus, including 2 × EasyTaq PCR
Supermix, primer pair, DNA profiling and ultrapure water；It is highly preferred that the present invention is for identifying arborescent ceratoids microsatellite locus
Kit in, including 50-100ngDNA template, 7.5ul2 × EasyTaq PCR Supermix (TransGen
Biotech), 10 μM of each 0.5ul of primer pair, use ultrapure water polishing to 15ul；It is highly preferred that the PCR condition of the kit
For 95 DEG C of initial denaturation 5min；95 DEG C of denaturation 30sec are suitable for anneal under annealing temperature 30sec, 72 DEG C of extension 30sec, react 32
Circulation；72 DEG C of extension 10min；Wherein, the suitable annealing temperature is 50 DEG C~61 DEG C；Most preferably, described suitable to move back
Fiery temperature is 50 DEG C, 51 DEG C, 52 DEG C, 53 DEG C, 54 DEG C, 55 DEG C, 56 DEG C, 57 DEG C, 58 DEG C, 59 DEG C, 60 DEG C, 61 DEG C.
Preferably, the identification of the arborescent ceratoids is the following steps are included: with aforementioned kit to the gene of sample to be tested
Group DNA carry out PCR amplification, amplified production and microsatellite sequence are compared: if containing described in above-mentioned technical proposal at least
A kind of microsatellite molecular marker then determines the sample to be tested for arborescent ceratoids；If without containing described in above-mentioned technical proposal
Any one microsatellite molecular marker then determines that the sample to be tested is not arborescent ceratoids.
Another aspect of the invention is to provide the sieve of the microsatellite locus and its specific primer pair of above-mentioned arborescent ceratoids
Choosing method, comprising the following steps:
1) it collects arborescent ceratoids sample and extracts DNA；
2) DNA obtained to step 1) constructs RNA-seq cDNA library, and carries out to the RNA-seq cDNA library
High-flux sequence；
3) it detects microsatellite locus and designs the primer pair of microsatellite locus；
4) it screens and detects microsatellite locus and its specific primer pair.
Preferably, in the microsatellite locus of arborescent ceratoids of the invention and its screening technique of specific primer pair,
It is CTAB method that DNA step is extracted in the step 1)；
Preferably, in the microsatellite locus of arborescent ceratoids of the invention and its screening technique of specific primer pair,
The high-flux sequence of RNA-seq cDNA library carries out high pass using lumina Hiseq3000 microarray dataset in the step 2)
Measure sequence；
Preferably, in the microsatellite locus of arborescent ceratoids of the invention and its screening technique of specific primer pair,
The Sequence Detection microsatellite that the step 2) is completed using batchprimer3 on-line analysis tool in the step 3)
Site sequence simultaneously carries out design of primers on the flanking sequence of microsatellite locus；It is highly preferred that the microsatellite locus detection
Condition is repetitive unit when being 2 bases, and number of repetition need to be more than or equal to 6；When repetitive unit is 3 bases, number of repetition is needed
More than or equal to 5；When repetitive unit is 4,5 and 6 bases, number of repetition need to be more than or equal to 4；It is highly preferred that the microsatellite position
The principle of the specific primer design of point is that amplification target fragment is 100~200bp, and primer G/C content is 40~60%, up and down
Primer annealing temperature (Tm) is between 50~60 DEG C and difference is less than 5 DEG C for trip.
Preferably, in the microsatellite locus of arborescent ceratoids of the invention and its screening technique of specific primer pair,
The method of the screening microsatellite specific primer in the step 4) are as follows: step 3) is obtained using arborescent ceratoids DNA
Specific primer is to progress PCR amplification and detects, and screening, which can stablize, amplifies target stripe and the primer pair with polymorphism；
Preferably, in the microsatellite locus of arborescent ceratoids of the invention and its screening technique of specific primer pair,
The detection microsatellite specific primer method in the step 4) are as follows: using 350 fluorescent molecule amount standard of Tamara in ABI
Genotyping is carried out to the primer pair screened using polyacrylamide gel in 3730 DNA sequencers；The knot of Genotyping
Fruit is read out using GENMARKER software, and inputs Excel file；Use Excel Mircosatellite Tool kit
Program counts expectation heterozygosity (the Expected Heterozygosity, H of each microsatellite locusE), observation heterozygosity
(Observed Heterozygosity, HO) and polymorphism information content (Polymorphic information content,
PIC), to detect the polymorphism situation of the microsatellite locus, filter out above three polymorphism numerical value and be all larger than 0.5 as micro-
The primer pair in satellite site.
Therefore, the present invention provides the microsatellite locus of arborescent ceratoids and its specific primer plants in Chenopodiaceae ceratoides
Genetic marker, positioning and the qtl analysis of strain, cultivar identification, the application in population and Study on Evolution, molecular mark.
Compared with prior art, the present invention present invention firstly discloses 22 specific microsatellite sequences of arborescent ceratoids
And 22 pairs of specific micro-satellite primers.These sites to arborescent ceratoids analysis of genetic diversity, conservative genetics research,
It plays an important role in Germplasm Resource Investigation and assistant breeding, also provides basic material to study the research of plant drought mechanism.
Specific embodiment
Below in conjunction with the embodiment in the present invention, the technical solution in the present invention is clearly and completely described.It is aobvious
So, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the reality in the present invention
Example is applied, every other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to
In the scope of protection of the invention.
Embodiment 1
1, DNA is extracted
Preservation in silica is used after sample acquisition, is used altogether to 32 parts of arborescent ceratoids samples, is acquired from Alashan Area, inner Mongolia Autonomous Region
Zuo Qi.Sample carries out DNA extracting using CTAB method.
2, Jian Ku, high-flux sequence and assembling
Use illumina TruSeq Stranded Total RNA Sample Prep Kit (brilliant energy biotechnology
(Shanghai) Co., Ltd.) building arborescent ceratoids the library standard rna-seqcDNA；It is surveyed using illumina Hiseq 3000
The both-end sequencing mode of sequence platform carries out high-flux sequence to the RNA-seq cDNA library of building.To by Quality Control Analysis
High quality sequence afterwards carries out sample assembling.92629 original series (unigene) are obtained after sequence is assembled.
3, microsatellite sequence detection and design of primers
The Sequence Detection microsatellite locus sequence that the step 2 is completed using batchprimer3 on-line analysis tool
It arranges and carries out design of primers on the flanking sequence of microsatellite locus；The condition of the microsatellite locus detection is that repetitive unit is
When 2 bases, number of repetition need to be more than or equal to 6；When repetitive unit is 3 bases, number of repetition need to be more than or equal to 5；It repeats single
When member is 4,5 and 6 bases, number of repetition need to be more than or equal to 4；The principle of the design of primers of the microsatellite locus is amplification mesh
Segment be 100~200bp, primer G/C content be 40~60%, upstream and downstream primer annealing temperature (Tm) is between 50~60 DEG C
And difference is less than 5 DEG C.
With this condition, 7534 microsatellite sequences are detected altogether, are designed into 5978 pairs of primer pairs.
4, the preliminary screening of micro-satellite primers
It is determining to carry out PCR amplification condition for 50 primer pairs of selection in 5978 pairs of successful primer pairs of design.The original of selection
Be then: the preferred base units that repeat are 2 or 3 (this kind of microsatellite polymorphism is higher)；It is preferred that repetitive sequence is without nucleotide variation, nothing
It is inserted into or lacks；Preferred repeat units number big (but being no more than 50).
DNA using 8 arborescent ceratoids samples is template, carries out grads PCR amplification to this 50 primer pairs.Gradient
PCR reaction system is as follows: 50-100ngDNA template, 7.5ul2 × EasyTaq PCRSupermix (TransGen
Biotech), 10 μM of each 0.5ul of upstream and downstream primer, use ultrapure water polishing to 15ul.Grads PCR reaction cycle is provided that
95 DEG C of initial denaturation 5min；95 DEG C of denaturation 30sec, anneal within the scope of 45~65 DEG C 30sec under 12 gradient temperatures, 72 DEG C of extensions
15sec reacts 32 circulations；Last 72 DEG C of extensions 10min.12 gradients of the present invention are preferably 50 DEG C, 51 DEG C, 52 DEG C,
53℃、54℃、55℃、56℃、57℃、58℃、59℃、60℃、61℃。
The amplified production that grads PCR obtains uses 2% agarose-EB detected through gel electrophoresis, and selection meets the following conditions
Primer pair as preliminary screening primer pair: (1) amplified production be single band；(2) clip size meets target fragment size；
(3) amplification obtains consistent results in 8 individuals.Record meets PCR annealing temperature when above-mentioned condition, is denoted as the primer pair
Most suitable annealing temperature.
With this condition, 25 primary dcreening operation primer pairs are obtained.
4, the polymorphic detection of microsatellite locus
Fluorescent marker (FAM/HEX/TAMRA) is added to the end of upstream primer 5 ' of above-mentioned primary dcreening operation primer pair, and uses 32
The DNA of arborescent ceratoids sample is expanded.PCR reaction system are as follows: 50-100ngDNA template, 7.5ul2 × EasyTaq PCR
Supermix (TransGen Biotech), 10 μM of each 0.5ul of upstream and downstream primer, uses ultrapure water polishing to 15ul.Circulation is set
It is set to: 95 DEG C of initial denaturation 5min；95 DEG C of denaturation 30sec, anneal under most suitable annealing temperature 30sec, 72 DEG C of extension 15sec, reaction
35 circulations；72 DEG C of extension 10min.
Amplified production uses polypropylene using 350 fluorescent molecule amount standard of Tamara in 3730 DNA sequencer of ABI
Acrylamide gel carries out Genotyping.The result of genetic analysis is read out using GENMARKE software, and inputs Excel file.
Using Excel Mircosatellite Tool kit program calculate expectation heterozygosity (Expected Heterozygosity,
HE), observation heterozygosity (Observed Heterozygosity, HO) and polymorphism information content (Polymorphic
Information content, PIC), to detect the polymorphism situation of the microsatellite locus, select above three polymorphism
Numerical value is all larger than 0.5 site.
Finally obtain 22 stable high polymorphic micro-satellite sites, the microsatellite locus be named as T1, T6, T11,
T13, T15, T16, T17, T20, T22, T23, T25, T26, T28, T29, T30, T31, T34, T35, T36, T37, T38 and T39；
The T1, T6, T11, T13, T15, T16, T17, T20, T22, T23, T25, T26, T28, T29, T30, T31, T34, T35,
The nucleotide sequence of T36, T37, T38 and T39 sequence as shown in SEQIDNO1~SEQIDNO22 respectively；The T1, T6, T11,
T13, T15, T16, T17, T20, T22, T23, T25, T26, T28, T29, T30, T31, T34, T35, T36, T37, T38 and T39；
The T1, T6, T11, T13, T15, T16, T17, T20, T22, T23, T25, T26, T28, T29, T30, T31, T34, T35,
T36, T37, T38 and T39 primer pair are successively are as follows:
T1 primer pair sequence is as shown in SEQ ID NO.23 and SEQ ID NO.24；
T6 primer pair sequence is as shown in SEQ ID NO.25 and SEQ ID NO.26；
T11 primer pair sequence is as shown in SEQ ID NO.27 and SEQ ID NO.28；
T13 primer pair sequence is as shown in SEQ ID NO.29 and SEQ ID NO.30；
T15 primer pair sequence is as shown in SEQ ID NO.31 and SEQ ID NO.32；
T16 primer pair sequence is as shown in SEQ ID NO.33 and SEQ ID NO.34；
T17 primer pair sequence is as shown in SEQ ID NO.35 and SEQ ID NO.36；
T20 primer pair sequence is as shown in SEQ ID NO.37 and SEQ ID NO.38；
T22 primer pair sequence is as shown in SEQ ID NO.39 and SEQ ID NO.40；
T23 primer pair sequence is as shown in SEQ ID NO.41 and SEQ ID NO.42；
T25 primer pair sequence is as shown in SEQ ID NO.43 and SEQ ID NO.44；
T26 primer pair sequence is as shown in SEQ ID NO.45 and SEQ ID NO.46；
T28 primer pair sequence is as shown in SEQ ID NO.47 and SEQ ID NO.48；
T29 primer pair sequence is as shown in SEQ ID NO.49 and SEQ ID NO.50；
T30 primer pair sequence is as shown in SEQ ID NO.51 and SEQ ID NO.52；
T31 primer pair sequence is as shown in SEQ ID NO.53 and SEQ ID NO.54；
T34 primer pair sequence is as shown in SEQ ID NO.55 and SEQ ID NO.56；
T35 primer pair sequence is as shown in SEQ ID NO.57 and SEQ ID NO.58；
T36 primer pair sequence is as shown in SEQ ID NO.59 and SEQ ID NO.60；
T37 primer pair sequence is as shown in SEQ ID NO.61 and SEQ ID NO.62；
T38 primer pair sequence is as shown in SEQ ID NO.63 and SEQ ID NO.64；
T39 primer pair sequence is as shown in SEQ ID NO.65 and SEQ ID NO.66.
The T1, T6, T11, T13, T15, T16, T17, T20, T22, T23, T25, T26, T28, T29, T30, T31,
T34, T35, T36, T37, T38 and T39；The T1, T6, T11, T13, T15, T16, T17, T20, T22, T23, T25, T26,
The genetic diversity data of T28, T29, T30, T31, T34, T35, T36, T37, T38 and T39 microsatellite locus are shown in Table 1.
Table 1: microsatellite locus Diversity Detection result
Provided by the present invention for the molecular labeling polymorphism number of the microsatellite locus of identification it can be seen from 1 data of table
According to being all larger than 0.5, genetic diversity sex investigation, Genetic Constitution of Population analysis, individual identification and affiliation mirror may be incorporated for
Surely equal genetic analysis.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
SEQUENCE LISTING
<110>Inner Mongolia Autonomous Region Academy of Agricultural and Livestock Husbandry
<120>a kind of arborescent ceratoids specificity microsatellite molecular marker and its application
<130>A Sida
<160> 66
<170> PatentIn version 3.5
<210> 1
<211> 499
<212> DNA
<213>arborescent ceratoids
<400> 1
cgttcaaaga aatgacatcg gaaaaacctt tgaaaggtgt ctggtgtggc tatatgtcat 60
ctaccgcgac catgattatt attattatta ttattattat tattattatt attattattg 120
tgtactacat cgttttgtaa gctgttggcg aatggataag ccctggaaga agcagtggtg 180
gtagaaaatg atgagaatcc tgatgatgct gcagttgaca acaattggac ttgggttgca 240
gtaccaccaa ttccacctcc acttcctcct tgaccttggt tttgcatgta ccattgccag 300
tttggtagac ttgctgagcc tgccattgct tctagctttt catgatcatt aactttgtcc 360
tcataagctg cagaccgacc tggaggtaag ccagaccaca taggaggttg tcgcgatgcc 420
aatggctgac catagagtgg tggtgcacca ggagtaaagt atcccccatt cacaggccat 480
ttcccattag caacatgaa 499
<210> 2
<211> 375
<212> DNA
<213>arborescent ceratoids
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tctcggcggt ttttcttctc ctcccccggc cgctccaact ccatcgttga ctccctctcc 60
ctctcctcct ccaacctact attatcttca ccaccgcggg aggttgaggc ggaggcggag 120
gcagagttat catcctcctc ctcctcctcc tccacggtgg aggtggccgg gggagtaccc 180
gtgcagacgt actcccccga cccctacgtg gacttcagac aatccatgca agagatggtg 240
gaagcacgtg agttaggaaa tctaggggtg aaacaagcat gggaatattt gcaagagtta 300
ctgttgagtt acctagcact gaatccaaag aacactcaca agtacattgt tggagctttt 360
actgaccttg ttctt 375
<210> 3
<211> 499
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<213>arborescent ceratoids
<400> 3
ccgtggtggg aaatggaaga ggagaaaagg cgcggagatg agcggagagg acacgtggca 60
ggagatgatg aggaggagga ggaggaggag aagggtggtg gtgtggcagt tgcaaagggt 120
aaacgaaggt tttcaagtaa ggcgcacgat agcggctacc atacgctaac aaaggagatg 180
ctgatgctga tattattgat gaatgtagta gaaatggcat ctcttttttt tcttgaatta 240
tttccttttt ttaaaaaaaa aaaaaatcaa gggtggatga agataatgga taaagagaag 300
gcagccattg ttgaaggctt gaagcaagga agagaggaca tttgtaatat ctgaatgaag 360
taaatactta tctagacctg gcaaaacaga ccggcaccaa ttacccgacc tgacataccc 420
attctaccca aaaatgccaa aatatgctga cccccgaacg atacctgaac cgaactgacc 480
cgacaataaa tgtgtgtaa 499
<210> 4
<211> 481
<212> DNA
<213>arborescent ceratoids
<400> 4
agtaggcctg tagcagtttc attggagttc catccgagca agtcgaacaa catcaagtgc 60
agcatagttg gtagttgtat ttttcatttt ttggttaatg ctgttttatt tgtgtttcct 120
accgtctgct tctagagtct gcatttcaaa atttccagtt tcctgtggcc atactatgat 180
gatgatgatg atgatgaaaa gctctcctgc atggggtatc gattgcaatg gggctacagc 240
gattataaaa ttctttttct tgcaaattta ggaccaggag aatgatttag agtactttgg 300
tactctcaat catgtgtgtt tttcttgtaa ataggattgt taatttggtc ggtcaagaat 360
aaaaatgttc taaatttatg tactttttta aaggggtcaa tgaacatttc taccttcagc 420
tgtgtacatt tgccatatag gagtaattct tgagctcgat cttgttttat tttaccttgg 480
t 481
<210> 5
<211> 451
<212> DNA
<213>arborescent ceratoids
<400> 5
catgagagat agagctacaa cctaatacct ctcatgcata tataacaaac tactaaaaca 60
tcaaaccaat aaaattctaa acccataata ataataataa taataataat aataatatgg 120
acctccaact acatacgatt aatacgaact cctattttat aacataaagg aaccgagggt 180
acgtgcaagt atttaataat ttgtaaaaac gtaataatat aagaccaact cctatattac 240
gtacgcacca tatagtacgt actcaataat ctttatttat ggaatagctg gaagaccacc 300
attcccagga ctagatccaa ctccaggacc accaaaacct cccaaaccac caccaggact 360
accgcccaag ccacctagtc ctcccaaacc accaggacta ccaccaccta aagacccaaa 420
accaccaagt ccaccaggat taccaccacc a 451
<210> 6
<211> 274
<212> DNA
<213>arborescent ceratoids
<400> 6
cgggcggagc tgcggcggca aacacggcgg atgtgggtaa tccggtggtg gcggaattgt 60
tgagggagga gtttaaagga gctgagggat tgagatttgg gagaagaaga gtgagaaagc 120
atgctttgag tggagccatg gaagattgct tgagaacagg ctgccattga agagttgaag 180
agagattgaa tctatgaatg ttgttgttgt tgttgttgtt gttgttgttg taatgtggaa 240
gacagtgtta gatatggggg tcgtttgttc ttcg 274
<210> 7
<211> 499
<212> DNA
<213>arborescent ceratoids
<400> 7
gccgccggca ccaccgtcta ccagctccta ccccctaccc tcccccccct ctccaccacc 60
actgccatct tttgtcccac caccaccacc accaccacca cctctccacc tccacccgag 120
caaccatcag ataaaatcct cgacaacggg gtcataaaac gatccttcta ccccattggg 180
accgccgcat ataacttcgt attcatgtca gcttatcgcg gcggtcccaa cactttcgct 240
gtcataggtc tatcatccaa aacccttcaa gacttcggtc atcctacata cacatgtgaa 300
taccaatcaa acaataatac ctcttcttca tatatagtac caggttataa gcttcctatg 360
gatgactttg gttacgcacg aatgtacatt atagcagtcg taaactgtac atttcctaca 420
caaaccgacc cttccaccgg cggccggctg ctcctccacg ccgccaccac caacggcggt 480
gtaaactcaa ccgacacca 499
<210> 8
<211> 542
<212> DNA
<213>arborescent ceratoids
<400> 8
tgacatctcg tcactgaacc ttaatgaaat cccgataaat aaataaataa ataaataaac 60
ttggtacagt gacattgagg aatgaggtta ctatcaaaaa tgttgcagtg aacaggtttg 120
caaatttcca atgaatattc atctacgttt ctgtgagggc tgtggcatgt tattgctgga 180
ggattacagt tggtacggaa cagatatagc cataaaattt acccagatca ttcaatagtc 240
aagtgtctac gatacaaggc gtattttaga agttcatagg acgaaactac aatcccaact 300
gaaggcccag cacgggcaac tcgaggactg attcctgtaa atagcccttt ccaacctcca 360
tccctccata tctccatcaa tgtatgtcgt gttgtcatcc tcatctgtct agctggatct 420
ttctctatct gcctccgagt tcttgcaaca tctagagggc atgtgacagc tgcagcaagg 480
gtgcctgcaa tgaatccacc agcaaaattt gcacctaaaa cacttgtcgc attcatttca 540
tc 542
<210> 9
<211> 397
<212> DNA
<213>arborescent ceratoids
<400> 9
ttttagataa aattgcattt catttttttg ataaatttgg atttttttag gtgaatttta 60
tttttatttt ttattttttt aattttatac aatgcagttg caaattattg ggttttcaat 120
tttttgttaa atcttacgga ttactgattt atttttatga atttggtttt ttttattagt 180
ttagaatttt agagaaaaaa tatcgttcaa cgatttgtag caattttgct cgctctcata 240
gaatctgatg gagctctcct tgaaagtgtc ttctttttaa ttaaaagaga tagattataa 300
ataggaataa aaataaataa ataaataaat aaataaaagg gatgggcggt tataaatagg 360
agttgggacg cagacgtagt tcaaattaaa attgaag 397
<210> 10
<211> 376
<212> DNA
<213>arborescent ceratoids
<400> 10
ttaaacggat agtcaggagg gaaatggatc gtcactagaa atactcctcc agcataaggg 60
ctatcagcag gacccattat tgttgcctgc caatgaaaca tgtcttcagc aacaggacca 120
gcgctgcaag aagtaggagg atccttctgg agatccttga gctccttcaa gatccgcttg 180
gaagccatcg ctggagaacg gcaccctaat tttcagtccg acggcggaaa tcgtttggaa 240
ggagggttga atttgaatga aggttgaatg aatgaatgaa tgaatgaaag aaaacccaaa 300
tccaaattat aagggagatt gattgtggtg tggtgtgggg taggctgctg cttcttcacg 360
cggacctgat ttttgg 376
<210> 11
<211> 448
<212> DNA
<213>arborescent ceratoids
<400> 11
tggaagccat ttgttgttgc cagtttggtt gttgaggatt ctgcctcatc tgttgttgct 60
gttgttgttg ttgttgctgc tgctgctgct gctgctgtaa caaaacacgc tgcatcggtg 120
gcatattctg caacatttga tgctgctgtc gtagcctctg ctgctgaatg atagcatgca 180
actgcgggtt atggctttgc aactgcattg gatcctgtct ctgcagcaat tgatgtaaga 240
cttgctgctg caaaatatct tcttgcttaa tatccagccg cggttttttt ctgcagatgg 300
gatagattag caggatcttg catgaatgaa ttgggattcc ttgaccccat agaatgtgtg 360
agttggcctg tttgagatgc tggcaataat gaagcaccag aagcaccttg cggaaactga 420
tgttgactct gaatagcttg ttgagagt 448
<210> 12
<211> 435
<212> DNA
<213>arborescent ceratoids
<400> 12
tggatatacc tagtcgggcc aactgctgga gctatatctg gtgcaatggt gtacaatctt 60
ataagattta cggaaaaacc attgcgcgag atcaccaaaa ccggatcttt ccttaggact 120
aagggttcgt ctgtaagtcg taatggttct tcccagtact aggatttaga ggagaaacaa 180
cttaatttta gagggtttta tttgatcata aaaagtgtaa aatcctttta actcaaaatg 240
tatgtcaact aagtcttcca tctttaaata ataagttaga tggttgatat atataatgta 300
gttgcattat gtacgtacgt atgtatgtat gtatgtatgt atgttatgtg ccgtgcctgc 360
tgtattagtt tagtgatgtt ttataaggtt tttaattgtg aggaagatgg ttgagctatt 420
gttatttgag tagat 435
<210> 13
<211> 524
<212> DNA
<213>arborescent ceratoids
<400> 13
tatagcaaga agtaaacatc aaataaatag aaaatcacaa aaataaataa ataaataaat 60
aaattcatta aataagagcc taatcaaaca tttcccaatt tcaagttctt aaattccagt 120
gaatcaactc acgcaacatt agtccaagtg tattcgcacc atttccatca ttcatacaaa 180
cattcaaata cgaaagcaga aattaaacag aaatcagaac caaaacctca aatccccaaa 240
aatgaattaa taacccaaga ataaattaaa aaaagaaaaa aaaaggtaca gcggagattg 300
aagaagggtt ttcggcgatg atagctatgg cggaaacgga gtacttgaat cctacccgaa 360
cctctttgaa ttcgtcgttt cactgcgaag acggtgtgac ggctgggctt ccggcgagaa 420
ggagagagga caccgtgacg atgggcggac gacgtgacag tgggcggaga aagcaagctg 480
cagctgtggt gtatttctta gtaggaggat gatatcctct tgtg 524
<210> 14
<211> 559
<212> DNA
<213>arborescent ceratoids
<400> 14
aataaagtca atgccttgta aaataatcaa caaaaacatt tagtccaact actcattttt 60
ttgttgtgaa gaacaaagat taagctcgct cgctcgctcg ctcgctccca ctcgtactgc 120
gcatttcatc ctctctcctc cttcctcatc aatggcgagc agtaatctcc ctcgcagaat 180
catcaaggaa actcagcgtc tcctcagtga accagcaccg ggaataagtg cttcaccatc 240
agaagacaat atgcggtatt ttaatgtcat gattcttggc ccttcacagt ctccatatga 300
aggaggagtg tttaagttgg aattattttt gcctgaggaa tatccaatgg cggctcctaa 360
ggttcgattt ctcactaaaa tatatcatcc caacattgat aagctaggga ggatatgcct 420
tgatattctt aaagacaagt ggagtcctgc tctccagata cgtactgtac tcctgagtat 480
tcaagcattg cttagtgctc caaaccctga tgaccctctg tctgagaaca ttgctaagca 540
ttggaaaacg gatgaagca 559
<210> 15
<211> 460
<212> DNA
<213>arborescent ceratoids
<400> 15
tgctccatgt ttattgagga acaacttata tgatgaaggc agagattctt gcttcaatat 60
gtaggcagac aatatttggg aggaagaaag acacatgagg aagatgtctc catgagccca 120
agttaacggt ttgcaaaagc ggccaaaccg cttgcttttg attccacagc gagatgccaa 180
tacagctgct cgcatgagga tatatatagc caagctatta tgttgtgtgt ttaacccagt 240
taatagcata gaaggcccag ctattccacc tgccagtaat gccctccatt ttgcagtcct 300
gaattacagt gtatccgact caaaacacta acacaagtgc tagaatatac aagtagaata 360
tttcattatg ctatccaaat ttatttattt atttatttat ttttaaaaaa acaccaacag 420
tgagggaagt taagtttgta tggccattaa aactcagaac 460
<210> 16
<211> 481
<212> DNA
<213>arborescent ceratoids
<400> 16
tcacgtcagc atcaggcagc aaatacaaat tcacacttgg atattggttc aactggatac 60
agattactat ttatggagat gattgaagga ttgtaaacca gtaaagaatc ttacaggaca 120
gtctacctag aacaagctta tcaattaagg cttcaggttc aactggatac agagtactgt 180
ttatggaagg tatttgaagg attagaaact gggaaagcct atccagaaca actttgatca 240
ttaaggcttc aaggaaaaaa aaaaaacaca cacacacaca cacacacaca taggcatgtg 300
cttatacctg tgagcaatga cacgtcttat gttactcaca tcaagctatc atgtctgcca 360
agaacgatta aatcatatag gaaagaccct ccagcagcac aatatatgca atggcagcta 420
gcagattctt aacaccctcc taaaaaatga aagctacaca gcgctcaaaa ctattttaag 480
a 481
<210> 17
<211> 338
<212> DNA
<213>arborescent ceratoids
<400> 17
ctgacaaaag attggagctt gaatagtaaa gaaagaccta aagttgagga cattagtgaa 60
gtgttaagag agtgcttatt gtttgtggaa aaaatgaaca aaattcagat ctacggcttc 120
tcgtgatgtt tcttggtcgt ggccagaaga gcattcacgt gcaattttct ggggttgtgt 180
tgctgggatg ttccaagttg gttggttggt tggttggttg ttgtgatttg ttatggttgg 240
tttacgagcg tggaaaggat atatatcgat attactattc gagcagtttg ataacggatt 300
gccttttttt ttctccttgc ggtttgtctc tgtagttg 338
<210> 18
<211> 463
<212> DNA
<213>arborescent ceratoids
<400> 18
aagaaaatta aatactaaat aaaatcccca actcttccag actgagtgag tgagtgagtg 60
agtgagtaag aacaaccaca tttcttaaaa atcctaatat ttttttaggg tttttgttct 120
tcatattata tttatgagct tcaactatgg atacgcctga gcgtaatcag atcggaaccc 180
ccgtttccaa attcgaggag tctccatttt caaacttcat caacagtctg tctccgatca 240
acccaacgaa gacgatacac gtttcacaga cgtttggctc gcttagtttt acttcacctc 300
catctgtttt tacttcaccc catgtgagcc tccagaagga ttataagctc ttacgaaggc 360
atctagtttc agatcttgcg aaaactcagc ttgtgtctga tgaaggaaac aaagattctg 420
ctgcgaaaca ggtttcagaa gagccgcagt ctgacagtct cgc 463
<210> 19
<211> 563
<212> DNA
<213>arborescent ceratoids
<400> 19
gtgagctact catgttatca gtgaagagag cattgaccat tccacgttat ttcaatgcta 60
tcacattttt cattctcatt cattcattca ttcattcatt ctcttgtctc tctctatttc 120
tcactctcaa ttactcctac cgcattgatt gcattaacct aaacacgcat cttgttcaac 180
tccagatggc ggttgacgat gatatgtgtg gcgacggaac ccactgtgct gaccatccct 240
accgcaccac ctcccccggc ggcggcggta tttgcgcttt ctgcctccaa gacaaactcg 300
gtaaactcgt ctcctcctct tcctcctcct tcttcattgc ctcctcttcc tcttcctccc 360
cttccttcag atctgacctc ccttccgcca cttctacctc caatgtcgtc ggtgtcgggc 420
cacctcccac caccaccaac aatatcagta gtagtcggca tcaccatcaa aatagtagta 480
gtaatagtaa tagtaatagt aatagtagta ctaatcagca acatcaacat catgaagttg 540
gaagtgggca tcatcatcat cat 563
<210> 20
<211> 253
<212> DNA
<213>arborescent ceratoids
<400> 20
gttaaattcg tctcgataat atttttcaaa atatcatttt tttataatta tttacataag 60
tagattttgg gatgttaatg gttgaaaaag tgtatttgta aacatgataa aagaaacatg 120
tcaactaaag cggaagtatg attaacattg ccattttatg acttttaact agttggaatt 180
tttttccttt atttatttat ttatttattt atgtatgtaa tgtttagcat agtcagttca 240
aggaatattg gag 253
<210> 21
<211> 561
<212> DNA
<213>arborescent ceratoids
<400> 21
ataaccctaa acccatactt ttctctcacc agccgcttta ctctctcttc tctcatcatt 60
tcccctcttt tgatgatctt ctcatctctt tgatctaaca tttatagaac ttgaagcaaa 120
gagattaaaa aaaatgttaa gttccggcaa ttcctcggtg gttctgtcaa tttgtagcaa 180
ggaaatccaa gaatattgct tgcttgcttg cttgcttgct tgttgttcaa agagagcagc 240
cacgaagtat taaaacacac actcaccgac attacttact cacagtcaca aatgtaattg 300
taaaattcag atttaaaaaa tattagatct taaaaaaaaa agatagtaaa aagaatgata 360
tttagatcca aaataaaatg agtagttgtt ggtggagagt tgatgctata tccatggtac 420
cattaagtta actgaagatc ttggtgatga aattttgctg ctgatgactt cggtcaaatt 480
cctcggttgg tgttttgttg tttacatttt tttttgggta ggaaacaatt tgctttactt 540
aattttagtt gtagatctag t 561
<210> 22
<211> 612
<212> DNA
<213>arborescent ceratoids
<400> 22
aaataatgca taaacaaaca aaaaatttaa aatgttacaa aaaaaaaaag accgatgtat 60
attaatcgtc tacctgaaga ttatataagc tttttattcc ctttagatat ttctaagtac 120
aatttagcca acatcaaggc agtcaataat atttcttcac tctttttttc tttgtatgtg 180
tctatgcata attcaacaaa taaatttttc actatcttcc aatctataat atgcaatcta 240
tttttaccat ggtaaaatat ctgctcgtca agaatcttac tgctgattgt aacaagagaa 300
acaaatctct cttaacattc ctaatcttca gttctccaat taaggactgg ccgcaaaatc 360
accgcatgaa aggccaataa tatgtaaatg cagttttcag gttcttccag gaatttgtga 420
gaagcaaagg gaaaacttaa ctatgagaaa agtagaataa gattaatcca gatacagaat 480
acctccctgt aaatgcaact cttgcgcctg ggatgagaaa ctcatctccc caccaaaaaa 540
taaataaata aataaataaa taaaaaatga agagaaactc atcctctttc tttgggcttc 600
accaaaatga aa 612
<210> 23
<211> 21
<212> DNA
<213>artificial sequence
<400> 23
gtgtctggtg tggctatatg t 21
<210> 24
<211> 21
<212> DNA
<213>artificial sequence
<400> 24
ctcagcaagt ctaccaaact g 21
<210> 25
<211> 21
<212> DNA
<213>artificial sequence
<400> 25
ctctcctcct ccaacctact a 21
<210> 26
<211> 20
<212> DNA
<213>artificial sequence
<400> 26
atctcttgca tggattgtct 20
<210> 27
<211> 20
<212> DNA
<213>artificial sequence
<400> 27
aaatggaaga ggagaaaagg 20
<210> 28
<211> 21
<212> DNA
<213>artificial sequence
<400> 28
tcctttgtta gcgtatggta g 21
<210> 29
<211> 21
<212> DNA
<213>artificial sequence
<400> 29
gcttctagag tctgcatttc a 21
<210> 30
<211> 21
<212> DNA
<213>artificial sequence
<400> 30
ctaaatcatt ctcctggtcc t 21
<210> 31
<211> 21
<212> DNA
<213>artificial sequence
<400> 31
acaacctaat acctctcatg c 21
<210> 32
<211> 21
<212> DNA
<213>artificial sequence
<400> 32
ccctcggttc ctttatgtta t 21
<210> 33
<211> 21
<212> DNA
<213>artificial sequence
<400> 33
ggagaagaag agtgagaaag c 21
<210> 34
<211> 21
<212> DNA
<213>artificial sequence
<400> 34
acccccatat ctaacactgt c 21
<210> 35
<211> 19
<212> DNA
<213>artificial sequence
<400> 35
accactgcca tcttttgtc 19
<210> 36
<211> 21
<212> DNA
<213>artificial sequence
<400> 36
gcgataagct gacatgaata c 21
<210> 37
<211> 21
<212> DNA
<213>artificial sequence
<400> 37
acatctcgtc actgaacctt a 21
<210> 38
<211> 21
<212> DNA
<213>artificial sequence
<400> 38
cctcacagaa acgtagatga a 21
<210> 39
<211> 21
<212> DNA
<213>artificial sequence
<400> 39
agctctcctt gaaagtgtct t 21
<210> 40
<211> 22
<212> DNA
<213>artificial sequence
<400> 40
ttttaatttg aactacgtct gc 22
<210> 41
<211> 19
<212> DNA
<213>artificial sequence
<400> 41
ggcaccctaa ttttcagtc 19
<210> 42
<211> 21
<212> DNA
<213>artificial sequence
<400> 42
accacaatca atctccctta t 21
<210> 43
<211> 20
<212> DNA
<213>artificial sequence
<400> 43
cagtttggtt gttgaggatt 20
<210> 44
<211> 20
<212> DNA
<213>artificial sequence
<400> 44
cagtttggtt gttgaggatt 20
<210> 45
<211> 23
<212> DNA
<213>artificial sequence
<400> 45
tgtcaactaa gtcttccatc ttt 23
<210> 46
<211> 23
<212> DNA
<213>artificial sequence
<400> 46
tgtcaactaa gtcttccatc ttt 23
<210> 47
<211> 20
<212> DNA
<213>artificial sequence
<400> 47
agcaagaagt aaacatcaaa 20
<210> 48
<211> 21
<212> DNA
<213>artificial sequence
<400> 48
gcgaatacac ttggactaat g 21
<210> 49
<211> 22
<212> DNA
<213>artificial sequence
<400> 49
caacaaaaac atttagtcca ac 22
<210> 50
<211> 20
<212> DNA
<213>artificial sequence
<400> 50
attctgcgag ggagattact 20
<210> 51
<211> 21
<212> DNA
<213>artificial sequence
<400> 51
cattttgcag tcctgaatta c 21
<210> 52
<211> 21
<212> DNA
<213>artificial sequence
<400> 52
caaacttaac ttccctcact g 21
<210> 53
<211> 21
<212> DNA
<213>artificial sequence
<400> 53
atggaaggta tttgaaggat t 21
<210> 54
<211> 21
<212> DNA
<213>artificial sequence
<400> 54
acataagacg tgtcattgct c 21
<210> 55
<211> 21
<212> DNA
<213>artificial sequence
<400> 55
aaattcagat ctacggcttc t 21
<210> 56
<211> 21
<212> DNA
<213>artificial sequence
<400> 56
ctcgtaaacc aaccataaca a 21
<210> 57
<211> 21
<212> DNA
<213>artificial sequence
<400> 57
ctcgtaaacc aaccataaca a 21
<210> 58
<211> 20
<212> DNA
<213>artificial sequence
<400> 58
ctcaggcgta tccatagttg 20
<210> 59
<211> 21
<212> DNA
<213>artificial sequence
<400> 59
tccacgttat ttcaatgcta t 21
<210> 60
<211> 21
<212> DNA
<213>artificial sequence
<400> 60
acaagatgcg tgtttaggtt a 21
<210> 61
<211> 21
<212> DNA
<213>artificial sequence
<400> 61
gcggaagtat gattaacatt g 21
<210> 62
<211> 21
<212> DNA
<213>artificial sequence
<400> 62
ctccaatatt ccttgaactg a 21
<210> 63
<211> 21
<212> DNA
<213>artificial sequence
<400> 63
gtggttctgt caatttgtag c 21
<210> 64
<211> 21
<212> DNA
<213>artificial sequence
<400> 64
aagtaatgtc ggtgagtgtg t 21
<210> 65
<211> 21
<212> DNA
<213>artificial sequence
<400> 65
agaatacctc cctgtaaatg c 21
<210> 66
<211> 21
<212> DNA
<213>artificial sequence
<400> 66
ccaaagaaag aggatgagtt t 21
Claims (2)
1. for identifying the specific primer pair of arborescent ceratoids microsatellite locus, the microsatellite locus are as follows: T1, T6, T11,
T13, T15, T16, T17, T20, T22, T23, T25, T26, T28, T29, T30, T31, T34, T35, T36, T37, T38 and T39
One or more of, sequence is respectively as shown in SEQ ID NO1~SEQ ID NO22；
The nucleotide sequence of the specific primer pair is respectively as follows:
T1 primer pair sequence is as shown in SEQ ID NO.23 and SEQ ID NO.24；
T6 primer pair sequence is as shown in SEQ ID NO.25 and SEQ ID NO.26；
T11 primer pair sequence is as shown in SEQ ID NO.27 and SEQ ID NO.28；
T13 primer pair sequence is as shown in SEQ ID NO.29 and SEQ ID NO.30；
T15 primer pair sequence is as shown in SEQ ID NO.31 and SEQ ID NO.32；
T16 primer pair sequence is as shown in SEQ ID NO.33 and SEQ ID NO.34；
T17 primer pair sequence is as shown in SEQ ID NO.35 and SEQ ID NO.36；
T20 primer pair sequence is as shown in SEQ ID NO.37 and SEQ ID NO.38；
T22 primer pair sequence is as shown in SEQ ID NO.39 and SEQ ID NO.40；
T23 primer pair sequence is as shown in SEQ ID NO.41 and SEQ ID NO.42；
T25 primer pair sequence is as shown in SEQ ID NO.43 and SEQ ID NO.44；
T26 primer pair sequence is as shown in SEQ ID NO.45 and SEQ ID NO.46；
T28 primer pair sequence is as shown in SEQ ID NO.47 and SEQ ID NO.48；
T29 primer pair sequence is as shown in SEQ ID NO.49 and SEQ ID NO.50；
T30 primer pair sequence is as shown in SEQ ID NO.51 and SEQ ID NO.52；
T31 primer pair sequence is as shown in SEQ ID NO.53 and SEQ ID NO.54；
T34 primer pair sequence is as shown in SEQ ID NO.55 and SEQ ID NO.56；
T35 primer pair sequence is as shown in SEQ ID NO.57 and SEQ ID NO.58；
T36 primer pair sequence is as shown in SEQ ID NO.59 and SEQ ID NO.60；
T37 primer pair sequence is as shown in SEQ ID NO.61 and SEQ ID NO.62；
T38 primer pair sequence is as shown in SEQ ID NO.63 and SEQ ID NO.64；
T39 primer pair sequence is as shown in SEQ ID NO.65 and SEQ ID NO.66.
2. it is as described in claim 1 for identifying the specific primer pair of arborescent ceratoids microsatellite locus, in Chenopodiaceae camel's hair
In genetic marker, positioning and the qtl analysis of Chenopodium plant, cultivar identification, population and Study on Evolution, molecular mark
Using.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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| SSR和ISSR标记及其在牧草遗传与;解新明;《草业科学》;20051231;第22卷(第2期);第30-36页 * |
| 内蒙古野生华北驼绒藜种群遗传多样性和遗传分化研究;王普昶;《草业学报》;20110831;第20卷(第4期);摘要、第289页 2 结果与分析 * |
| 华北驼绒藜种子发育过程中内源激素变化动态的研究;韩智龙;《种子》;20081230;第27卷(第9期);第7-9页 * |
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