CN107197635A - Improve the biological agent of soil environment and improve the method for soil environment using it - Google Patents
Improve the biological agent of soil environment and improve the method for soil environment using it Download PDFInfo
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- CN107197635A CN107197635A CN201610155433.3A CN201610155433A CN107197635A CN 107197635 A CN107197635 A CN 107197635A CN 201610155433 A CN201610155433 A CN 201610155433A CN 107197635 A CN107197635 A CN 107197635A
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- soil
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Soil Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Mechanical Engineering (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Mycology (AREA)
- Botany (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Materials Engineering (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
Improve the cultural method of soil environment biological agent the invention discloses a kind of, comprise the following steps:By gigasporarosea, gigasporasp, these four strains of glomus mosseae, glomussp are handled, single spore surface sterilizing, are then transferred into culture dish respectively, add MES buffer solutions, Gel Gro;Using clover host, vermiculite and perlite volume ratio 1:1 mixed culture medium is as matrix, and length is transferred along host and matrix the Gel Gro dielectric surfaces for sprouting spore after two weeks;All hosts sprout sporogenesis mycorhiza with single respectively;And obtain unit cell fungi；Wood chip is made into sack with gauze to install, fully soaked in the bacterium solution for being put into above-mentioned fungi；2 bag bacterium bags are mixed with one ton of water, 1 kilogram every bag, bacterium bag is put into ton bucket, should not scatter, sufficient convection current is carried out with circulating pump, bacterium bag is taken out after 6 hours, the sheep manure that 50KG fermented is put into, is stirred in ton bucket with agitator, precipitation is placed after 1 hour, the 2/3 of top, as finished product are taken out after 24 hours.
Description
Technical field
The present invention relates to field of biological, more particularly to a kind of biological agent for improving soil environment and the method using its improvement soil environment.
Background technology
The problem of generally existing one is referred to as " continuous cropping obstacle " in domestic agriculture field now, i.e., continuous cultivation obstacle.In the plantation for being in particular in the crops of high added value, such as watermelon, muskmelon, strawberry.The form of expression is, if N has planted the crop, N+1 can not continue to plant the crop, if planted by force, yield can be greatly lowered, such case can be increasingly severe over time, N+3 is for example arrived, yield may be reduced to original 30% or so.
The basic reason of this problem has at 2 points, and one is that fertilizer crosses Sheng and causes soil hardening, and two be the residual of the disease bacterium of crop.Present peasant for such issues that solution be also two kinds, one is crop rotation, i.e., Second Year kind economic value is not very high thing, for example corn, rape.Another is field of having a rest, i.e., this block of Second Year temporarily without leaving unused and conserving.But both ways are all keeped away from the enemy's main force and struck where the enemy is the weakest, although soil activation can slightly recover, yield and economic benefit but significantly decline.
The content of the invention
Technical problem to be solved of the embodiment of the present invention is that there is provided a kind of preparation method of the biological agent for the improvement soil environment that can be eliminated to the continuous cropping obstacle of soil.
It is described to improve the cultural method of soil environment biological agent, comprise the following steps:
(1) by gigasporarosea, gigasporasp, these four strains of glomus mosseae, glomus sp are handled respectively, single spore surface sterilizing, are then transferred into culture dish, add MES buffer solutions, Gel-Gro;
(2) clover host, vermiculite and perlite volume ratio 1 are used:1 mixed culture medium is as matrix, and wherein matrix granule diameter is in 0.65 millimeter, and acid-base value is controlled between pH5 ~ 7;After long two weeks, host and matrix are transferred along the Gel-Gro dielectric surfaces for sprouting spore;All hosts sprout sporogenesis mycorhiza with single respectively;And obtain unit cell fungi；
(3) wood chip is made into sack with gauze to install, fully soaked in the bacterium solution for being put into above-mentioned fungi, China fir sack dries in the shade afterwards, degree of drying is placed on blank sheet of paper with sack still can leave watermark, but do not extrude and will not flow out liquid and be advisable；
(4) 2 bag bacterium bags are mixed with one ton of water, 1 kilogram every bag, bacterium bag is put into ton bucket, should not scatter, sufficient convection current is carried out with circulating pump, bacterium bag is taken out after 6 hours, the sheep manure that 50KG fermented is put into, is stirred in ton bucket with agitator, precipitation is placed after 1 hour, the 2/3 of top, as finished product are taken out after 24 hours.
As the further improvement of the technical program, further bred after the step 2：Matrix is:Soil=3:1 matrix, passes through the moist heat sterilization of 125 degree of facility 1 ~ 2 hour before transfer；Host plant is sorghum, and planting density is 60 plants/basin.
A diameter of 5 centimetres of the culture dish；Add 10 milliliters of 10mM MES buffer solutions, 0.04 gram of Gel-Gro.
Remaining part is handled in the step 4 by additional used bacterium bag and supplement water to carry out Second propagation, through Germicidal efficacy, the concentration of the active bacteria of the biological agent finally obtained can be reduced with the increase of breeding number of times, be lost activity substantially after being bred at three times.
The embodiment of the present invention, which additionally provides a kind of biological agent prepared using the cultural method for improving soil environment biological agent, improves the method for soil environment, comprises the following steps：
(1) if soil serious continuous cropping obstacle originally, then need to temporarily cease farming, soil deep-cut one meter with machinery is deep-cut, after the soil dug out is smashed, plant ash, fermentation sheep manure and biological agent are put into, is sufficiently stirred for afterwards, soil is calmed down to normal level, black mulch film is covered, after placing 3 ~ 6 months, continuous cropping problem is to solve；
(2) if soil can still be used originally, without stopping farming, during daily water, biological agent and irrigation water are pressed 1:After the modulation of 300 ratios, normal irrigation, with the number of times increase of irrigation, soil activation can gradually recover, and continuous cropping obstacle will not occur.
Implement the embodiment of the present invention, have the advantages that：
The embodiment of the present invention is combined bacteria agent with irrigation water, and soil continuous cropping is solved the problems, such as during normal irrigation；For serious unbalance soil, combined using the technology of deep-cutting of reinforcing with bacteria agent, by soil restoration activity within six months；Ensure that high value crop after using our technology, can continuously be planted without crop rotation or field of having a rest every year.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, below the present invention is described in further detail.
The embodiment of the present invention improves the cultural method of soil environment biological agent, comprises the following steps:
(1) by gigasporarosea, gigasporasp, these four strains of glomus mosseae, glomus sp are handled respectively, single spore surface sterilizing, are then transferred into culture dish, a diameter of 5 centimetres of culture dish；Add 10 milliliters of 10mM MES buffer solutions, 0.04 gram of Gel-Gro;
(2) clover host, vermiculite and perlite volume ratio 1 are used:1 mixed culture medium is as matrix, and wherein matrix granule diameter is in 0.65 millimeter, and acid-base value is controlled between pH5 ~ 7;After long two weeks, host and matrix are transferred along the Gel-Gro dielectric surfaces for sprouting spore;All hosts sprout sporogenesis mycorhiza with single respectively;And obtain unit cell fungi；
(3) wood chip is made into sack with gauze to install, fully soaked in the bacterium solution for being put into above-mentioned fungi, China fir sack dries in the shade afterwards, degree of drying is placed on blank sheet of paper with sack still can leave watermark, but do not extrude and will not flow out liquid and be advisable；
(4) 2 bag bacterium bags are mixed with one ton of water, 1 kilogram every bag, bacterium bag is put into ton bucket, should not scatter, sufficient convection current is carried out with circulating pump, bacterium bag is taken out after 6 hours, the sheep manure that 50KG fermented is put into, is stirred in ton bucket with agitator, precipitation is placed after 1 hour, the 2/3 of top, as finished product are taken out after 24 hours；
Remaining part is handled by additional used bacterium bag and supplement water to carry out Second propagation, through Germicidal efficacy, the concentration of the active bacteria of the biological agent finally obtained can be reduced with the increase of breeding number of times, be lost activity substantially after breeding at three times.
The embodiment of the present invention, which additionally provides a kind of biological agent prepared using the cultural method for improving soil environment biological agent, improves the method for soil environment, comprises the following steps：
(1) if soil serious continuous cropping obstacle originally, then need to temporarily cease farming, soil deep-cut one meter with machinery is deep-cut, after the soil dug out is smashed, plant ash, fermentation sheep manure and biological agent are put into, is sufficiently stirred for afterwards, soil is calmed down to normal level, black mulch film is covered, after placing 3 ~ 6 months, continuous cropping problem is to solve；
(2) if soil can still be used originally, without stopping farming, during daily water, biological agent and irrigation water are pressed 1:After the modulation of 300 ratios, normal irrigation, with the number of times increase of irrigation, soil activation can gradually recover, and continuous cropping obstacle will not occur.
The embodiment of the present invention is combined bacteria agent with irrigation water, and soil continuous cropping is solved the problems, such as during normal irrigation；For serious unbalance soil, combined using the technology of deep-cutting of reinforcing with bacteria agent, by soil restoration activity within six months；Ensure that high value crop after using our technology, can continuously be planted without crop rotation or field of having a rest every year.
Above disclosed is only a kind of preferred embodiment of the invention, can not limit the interest field of the present invention, therefore the equivalent variations made according to the claims in the present invention with this certainly, still belongs to the scope that the present invention is covered.
Claims (5)
1. a kind of improve the cultural method of soil environment biological agent, it is characterised in that, comprise the following steps:
(1)
By gigasporarosea, gigasporasp, these four strains of glomus mosseae, glomus sp are handled respectively, single spore surface sterilizing, are then transferred into culture dish, add MES buffer solutions, Gel-Gro;
(2)
Using clover host, vermiculite and perlite volume ratio 1:1 mixed culture medium is as matrix, and wherein matrix granule diameter is in 0.65 millimeter, and acid-base value is controlled between pH5 ~ 7;After long two weeks, host and matrix are transferred along the Gel-Gro dielectric surfaces for sprouting spore;All hosts sprout sporogenesis mycorhiza with single respectively;And obtain unit cell fungi；
(3) wood chip is made into sack with gauze to install, fully soaked in the bacterium solution for being put into above-mentioned fungi, China fir sack dries in the shade afterwards, degree of drying is placed on blank sheet of paper with sack still can leave watermark, but do not extrude and will not flow out liquid and be advisable；
(4) 2 bag bacterium bags are mixed with one ton of water, 1 kilogram every bag, bacterium bag is put into ton bucket, should not scatter, sufficient convection current is carried out with circulating pump, bacterium bag is taken out after 6 hours, the sheep manure that 50KG fermented is put into, is stirred in ton bucket with agitator, precipitation is placed after 1 hour, the 2/3 of top, as finished product are taken out after 24 hours.
2. improve the cultural method of soil environment biological agent as claimed in claim 1, it is characterised in that, further bred after the step 2：Matrix is:Soil=3:1 matrix, passes through the moist heat sterilization of 125 degree of facility 1 ~ 2 hour before transfer；Host plant is sorghum, and planting density is 60 plants/basin.
3. improve the cultural method of soil environment biological agent as claimed in claim 1, it is characterised in that, a diameter of 5 centimetres of the culture dish；Add 10 milliliters of 10mM MES buffer solutions, 0.04 gram of Gel-Gro.
4. improve the cultural method of soil environment biological agent as claimed in claim 1, it is characterized in that, remaining part is handled in the step 4 by additional used bacterium bag and supplement water to carry out Second propagation, through Germicidal efficacy, the concentration of the active bacteria of the biological agent finally obtained can be reduced with the increase of breeding number of times, be lost activity substantially after being bred at three times.
5. a kind of biological agent prepared using the cultural method as claimed in claim 1 for improving soil environment biological agent improves the method for soil environment, it is characterised in that comprise the following steps：
(1) if soil serious continuous cropping obstacle originally, then need to temporarily cease farming, soil deep-cut one meter with machinery is deep-cut, after the soil dug out is smashed, plant ash, fermentation sheep manure and biological agent are put into, is sufficiently stirred for afterwards, soil is calmed down to normal level, black mulch film is covered, after placing 3 ~ 6 months, continuous cropping problem is to solve；
(2) if soil can still be used originally, without stopping farming, during daily water, biological agent and irrigation water are pressed 1:After the modulation of 300 ratios, normal irrigation, with the number of times increase of irrigation, soil activation can gradually recover, and continuous cropping obstacle will not occur.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610155433.3A CN107197635A (en) | 2016-03-18 | 2016-03-18 | Improve the biological agent of soil environment and improve the method for soil environment using it |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610155433.3A CN107197635A (en) | 2016-03-18 | 2016-03-18 | Improve the biological agent of soil environment and improve the method for soil environment using it |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107197635A true CN107197635A (en) | 2017-09-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610155433.3A Pending CN107197635A (en) | 2016-03-18 | 2016-03-18 | Improve the biological agent of soil environment and improve the method for soil environment using it |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100998611A (en) * | 2006-12-28 | 2007-07-18 | 温州大学 | Huishuhua fermentation preparation process using Chinese herb medicine as medium |
| CN101012440A (en) * | 2006-12-18 | 2007-08-08 | 中国矿业大学(北京) | Bush mycorrhiza agent culturing method for improving ore district land reclaimation effect |
| US20080046277A1 (en) * | 2001-02-20 | 2008-02-21 | Stamets Paul E | Living systems from cardboard packaging materials |
| CN102776124A (en) * | 2012-07-03 | 2012-11-14 | 山西省农业科学院生物技术研究中心 | Compound microbes for reducing and disinfecting greenhouse soil and soil disinfection method |
| CN104450536A (en) * | 2014-11-13 | 2015-03-25 | 山东农业大学 | Separation of arbuscular mycorrhizal fungi as well as preparation and application of arbuscular mycorrhizal fungi fungicide |
-
2016
- 2016-03-18 CN CN201610155433.3A patent/CN107197635A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080046277A1 (en) * | 2001-02-20 | 2008-02-21 | Stamets Paul E | Living systems from cardboard packaging materials |
| CN101012440A (en) * | 2006-12-18 | 2007-08-08 | 中国矿业大学(北京) | Bush mycorrhiza agent culturing method for improving ore district land reclaimation effect |
| CN100998611A (en) * | 2006-12-28 | 2007-07-18 | 温州大学 | Huishuhua fermentation preparation process using Chinese herb medicine as medium |
| CN102776124A (en) * | 2012-07-03 | 2012-11-14 | 山西省农业科学院生物技术研究中心 | Compound microbes for reducing and disinfecting greenhouse soil and soil disinfection method |
| CN104450536A (en) * | 2014-11-13 | 2015-03-25 | 山东农业大学 | Separation of arbuscular mycorrhizal fungi as well as preparation and application of arbuscular mycorrhizal fungi fungicide |
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Application publication date: 20170926 |
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