CN107192581A - Quantitative sampling dilution device and its method - Google Patents
Quantitative sampling dilution device and its method Download PDFInfo
- Publication number
- CN107192581A CN107192581A CN201710377140.4A CN201710377140A CN107192581A CN 107192581 A CN107192581 A CN 107192581A CN 201710377140 A CN201710377140 A CN 201710377140A CN 107192581 A CN107192581 A CN 107192581A
- Authority
- CN
- China
- Prior art keywords
- sample
- capillary
- diluter
- supporting member
- back tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000005070 sampling Methods 0.000 title claims abstract description 32
- 239000012895 dilution Substances 0.000 title claims abstract description 28
- 238000010790 dilution Methods 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 10
- 238000000605 extraction Methods 0.000 claims abstract description 55
- 239000012898 sample dilution Substances 0.000 claims abstract description 19
- 238000003780 insertion Methods 0.000 claims abstract description 4
- 230000037431 insertion Effects 0.000 claims abstract description 4
- 238000007789 sealing Methods 0.000 claims description 20
- 239000005030 aluminium foil Substances 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 12
- 230000000903 blocking effect Effects 0.000 claims description 10
- 238000003825 pressing Methods 0.000 claims description 4
- 210000002421 cell wall Anatomy 0.000 claims description 3
- 238000003113 dilution method Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 230000006978 adaptation Effects 0.000 claims description 2
- 235000019994 cava Nutrition 0.000 claims 1
- 238000005842 biochemical reaction Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 73
- 238000010276 construction Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000000712 assembly Effects 0.000 description 3
- 238000000429 assembly Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000013461 design Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000012631 diagnostic technique Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012123 point-of-care testing Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004513 sizing Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
- G01N2001/1472—Devices not actuated by pressure difference
- G01N2001/149—Capillaries; Sponges
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
- G01N2001/386—Other diluting or mixing processes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Hydrology & Water Resources (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of quantitative sampling dilution device and its method, the device includes sample amounts extraction assembly and sample cut-back tank；Sample amounts extraction assembly includes the upper cover part and lower specimen extraction device that split is set, lower specimen extraction device includes capillary and the supporting member for supporting capillary, the upper end of supporting member can be adapted to upper cover part snapping, the lower end of supporting member with the openend of sample cut-back tank；The supporting member has the thief hole of insertion, and the upper lid that there is upper cover part outer wall shape to be adapted to thief hole inner wall shape is blocked；At the upper end of supporting member and upper cover part snapping, upper lid blocks thief hole；Capillary is configured at the inside of sample cut-back tank by supporting member.Therefore, the present invention can not only realize quantitative sample dilution, additionally it is possible to complete quantitative sample and pipette to kit progress biochemical reaction.
Description
Technical field
The present invention relates to a kind of quantitative sampling dilution device and its method, diluted for micro-example quantitative sampling and mixed
It is even.
Background technology
Immune lateral chromatography diagnostic techniques is adapted in various real-time test as a kind of stabilization and practical technology
（POCT）Or onsite application.
Existing quantitative sampling device, after sampling dilution is mixed, oozes drop, this design is in sizing from liquid droping port above
Because its simplicity is advantageous in the test of product.But if the sample-adding of basis weight products, due to operator have add or less plus
The risk of one or two drop quantitatively brings many uncertainties to product.Simultaneously as meeting during rotation is mixed above and below liquid
There is bubble generation, during dropping liquid the first drop of liquid can be caused to contain the inaccurate of bubble liquid volume added.
The content of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of quantitative sampling dilution device, its one side can be by certainly
The capillary of body configuration realizes accurate sampling, on the other hand can also have solid using absorption by the well that itself configures
Quantitative liquid-transfering gun is accurately loaded, so as to avoid the uncertainty of direct dropping liquid.
To realize above-mentioned technical purpose, the present invention will take following technical scheme：
A kind of quantitative sampling dilution device, including sample amounts extraction assembly and sample cut-back tank；The sample amounts are carried
Take component include split set upper cover part and lower specimen extraction device, lower specimen extraction device include capillary and
Supporting member for supporting capillary, the upper end of supporting member can be with upper cover part snapping, and the lower end of supporting member can
It is adapted to the openend of sample cut-back tank；The supporting member has the thief hole of insertion, and upper cover part has outer wall shape
The upper lid being adapted to thief hole inner wall shape is blocked；At the upper end of supporting member and upper cover part snapping, upper lid is blocked
Thief hole；Capillary is configured at the inside of sample cut-back tank by supporting member.
The supporting member lower end sets capillary pipe pig；The capillary pipe pig include isosceles triangle cambered surface support with
And the capillary mounting rod arranged along isosceles triangle cambered surface support center line, the lower arcuate surface of the isosceles triangle cambered surface support
The lower surface of supporting member is fixed on, and the two ends of lower arcuate surface are located on the center line of bottom blocking lower surface, sample simultaneously
Hole is in the inner side of isosceles triangle cambered surface support；Capillary is supported by capillary mounting rod.
The supporting member is adapted to down including outer wall shape with sample cut-back tank close to inner wall shape at aperture position
Portion is blocked, and thief hole is set along the length bearing of trend that bottom is blocked, and capillary pipe pig is installed on the lower end of bottom blocking.
The upper cover part includes capping, and the upper end of capping is provided with hollow upper lid pressing chamber, and the lower end of capping is set
There is described upper lid to block, and nestle up lid and block the upper lid fixed block of setting；The upper end that bottom is blocked has and capping outer rim
The lower flange of alignment, and bottom blocks setting inner wall shape and upper lid fixed block outer wall at position corresponding with upper lid fixed block
The bottom Blind locating holes of shape adaptation；When upper lid blocking is plugged in thief hole, placement above lower flange is close in capping, and
Upper lid fixed block is plugged in the Blind locating holes of bottom；Upper cover part passes through upper lid blocking and the grafting of thief hole, upper lid fixed block
The snapping with supporting member is realized in grafting with bottom Blind locating holes jointly.
The outward flange of the capping is provided with lid locating notch, lower flange position corresponding with upper lid locating notch
It is provided with bottom locating notch；Lower flange has two extraction assemblies point so that bottom locating notch is symmetrically disposed about simultaneously
From breach.
The section of the thief hole is circle, and the section of bottom Blind locating holes is prismatic, thief hole, bottom Blind locating holes
Side wall connect to be formed one have positive stop lug boss through hole.
Bottom on the outside of thief hole blocks and offers bottom dummy notch, and the bottom dummy notch is deep-slotted chip breaker, and
Caving in of having that shape is adapted to bottom Blind locating holes outer wall at the inner side cell wall position corresponding with bottom Blind locating holes is convex
Block, and deep-slotted chip breaker is with the Central Symmetry setting of bottom Blind locating holes.
The upper lid is blocked is provided with technique blind hole along cock body bearing of trend.
The sample cut-back tank includes diluter body, and the diluter body has an openend, and openend is to extension
Stretch to form diluter flange, the openend of diluter body is by sealing aluminium foil sealing；Diluter body with diluter flange
Adjacent inwall is provided with diluter seal groove, and the bottom of supporting member blocks the sealing by being installed in diluter seal groove
The inner wall sealing of part and diluter body, and the bottom of diluter body is arranged to conical lower portion, while diluter body is outer
Wall is provided with diluter outer wall anti-slip part, the annular lines that the anti-slip part is set for a plurality of outer wall along diluter body.
Another technical purpose of the present invention is to provide a kind of quantitative sampling dilution process, comprises the following steps：
（1）Sample dilution is dispensed and sample collection
Sample dilution is dispensed：Quantitative Sample dilution is contained in sample cut-back tank, and uses sealing aluminium foil by sample
The opening end closure of cut-back tank；
Sample collection：Using the capillary contact sample to be collected in sample amounts extraction assembly, capillary passes through capillary force
Effect quantitatively suck sample to be collected；
（2）Sample Dilution is mixed first, and the capillary in sample amounts extraction assembly is puncturing sample cut-back tank openend
Seal after aluminium foil, continue to move ahead, until the lower flange that supporting member bottom is blocked in sample amounts extraction assembly compresses sample
The diluter flange of cut-back tank openend, completes the assembling of sample amounts extraction assembly and sample cut-back tank；Now, support
The bottom of part blocks the sealing in the diluter seal groove for passing through and being installed on and nestling up set by sample cut-back tank openend
The inner wall sealing of part and sample cut-back tank；Then, upper end and the sample dilution of sample amounts extraction assembly are pressed respectively
The bottom of container, turns upside down repeatedly, until the sample in capillary is mixed with the Sample dilution in sample cut-back tank；
（3）Sample application
Upper cover part in sample amounts extraction assembly and lower specimen extraction device are separated, using quantitative liquid shifter by setting
It is placed in the thief hole on lower specimen extraction device and draws the sample after quantitative dilution, adds the follow-up biochemistry of kit progress anti-
Should.
According to above-mentioned technical scheme, relative to prior art, the present invention has following beneficial effect：
In immune series of strata reaction system, particularly in basis weight products, the accuracy of sample-adding amount is played an important role, while micro-
Requirement of the amount sampling (less than 20 microlitres) to sample injector is very high, and frequency of maintenance is also high.The present invention is micro- by capillary quantitative
The sample of volume is measured, and mixing is diluted in body, the purpose accurately sampled is played.Simultaneously fixed amount is drawn with liquid-transfering gun
Liquid carry out sample-adding operation, it is to avoid the uncertainty of direct dropping liquid.Direct dropping liquid has plus the wrong risk for dripping number, Er Qie
One drop has been possible to bubble, causes the inaccurate of sample-adding.
Brief description of the drawings
Fig. 1 a are the front views of quantitative sampling dilution device of the present invention；
Fig. 1 b are the top views of quantitative sampling dilution device of the present invention；
Fig. 1 c are the upward views of quantitative sampling dilution device of the present invention；
In Fig. 1 a, 1b, 1c：1st, upper cover part；2nd, lower specimen extraction device；3rd, sample cut-back tank；
Fig. 2 is the exploded view of quantitative sampling dilution device of the present invention；
In Fig. 2：1st, upper cover part；2nd, lower specimen extraction device；3rd, sample cut-back tank；4th, aluminium foil is sealed；
Fig. 3 a are the dimensional structure diagrams of upper cover part in sample amounts extraction assembly of the present invention；
Fig. 3 b are Fig. 3 a semi-cutaways；
Fig. 3 c are the front views of the upper cover part；
Fig. 3 d are the top views of upper cover part；
Fig. 3 e are the upward views of upper cover part；
In Fig. 3 a-3e：1-1, upper lid pressing chamber；1-2, capping；1-3, upper lid locating notch；1-4, upper lid are blocked；1-5, upper lid
Fixed block；1-6, technique blind hole；
Fig. 4 a are the dimensional structure diagrams of sample amounts extraction assembly middle and lower part sample extraction device of the present invention；
Fig. 4 b are the semi-cutaways of lower specimen extraction device described in Fig. 3 a；
Fig. 4 c are the top views of the lower specimen extraction device；
Fig. 4 d are the upward views of the lower specimen extraction device；
Fig. 4 e are Fig. 4 c front views；
Fig. 4 f are Fig. 4 d right views；
In Fig. 4 a-4f：2-1, lower flange；2-2, bottom are blocked；2-3, extraction assembly separation breach；2-4, bottom locating slot
Mouthful；2-5, bottom dummy notch；2-6, thief hole；2-7, bottom Blind locating holes；2-8, quantitative capillary tube；2-9, capillary tube stay
Frame；
Fig. 5 a are the dimensional structure diagrams of sample cut-back tank of the present invention；
Fig. 5 b are Fig. 5 a semi-cutaways；
Fig. 5 c are the front views of the sample cut-back tank；
Fig. 5 d are the top views of the sample cut-back tank；
Fig. 5 e are the upward views of the sample cut-back tank；
In Fig. 5 a-5e：3-1, diluter flange；3-2, diluter body；3-3, diluter outer wall anti-slip part；3-4, diluter are close
Sealing groove；3-5, diluter conical lower portion.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Below
Description only actually at least one exemplary embodiment is illustrative, is never used as to the present invention and its application or makes
Any limitation.Based on the embodiment in the present invention, those of ordinary skill in the art are not making creative work premise
Lower obtained every other embodiment, belongs to the scope of protection of the invention.Unless specifically stated otherwise, otherwise in these realities
Apply the part illustrated in example and the positioned opposite of step, expression formula and numerical value are not limited the scope of the invention.Meanwhile, should be bright
In vain, for the ease of description, the size of the various pieces shown in accompanying drawing is not to be drawn according to actual proportionate relationship.It is right
It may be not discussed in detail in technology, method and apparatus known to person of ordinary skill in the relevant, but in the appropriate case, institute
Stating technology, method and apparatus should be considered as authorizing a part for specification.In shown here and discussion all examples, appoint
What occurrence should be construed as merely exemplary, not as limitation.Therefore, the other examples of exemplary embodiment can
With with different values.
For the ease of description, space relative terms can be used herein, such as " ... on ", " ... top ",
" ... upper surface ", " above " etc., for describing such as a device shown in the figure or feature and other devices or spy
The spatial relation levied.It should be appreciated that space relative terms are intended to comprising the orientation except device described in figure
Outside different azimuth in use or operation.If for example, the device in accompanying drawing is squeezed, be described as " in other devices
It will be positioned as " under other devices or construction after the device of part or construction top " or " on other devices or construction "
Side " or " under other devices or construction ".Thus, exemplary term " ... top " can include " ... top " and
" in ... lower section " two kinds of orientation.The device can also other different modes positioning (being rotated by 90 ° or in other orientation).
As shown in Fig. 1 a, 1b, 1c and Fig. 2, quantitative sampling dilution device of the present invention includes two parts, respectively
For sample amounts extraction assembly and sample cut-back tank；Wherein：Sample amounts extraction assembly, sample cut-back tank are real in production
It can respectively produce, can also form a complete production network in trampling；But in use, both are to use cooperatively that the dilute of biological sample could be realized
Release.
Specifically, to realize the quantitative sampling and quantitative sample-adding during sample detection of sample dilution, sample of the present invention
The quantitative extraction assembly of product, split be arranged to above and below two parts, correspond to upper cover part and lower specimen extraction device, wherein：
Described upper cover part, as shown in Fig. 3 a-e, including capping, the upper end of capping is provided with hollow upper lid pressing chamber, envelope
Cover and block in the lower end setting of lid, and nestle up lid and block the upper lid fixed block of setting；In addition, for ease of product injection molding,
Upper lid of the present invention is blocked is provided with technique blind hole along cock body bearing of trend；And it is easy to upper cover part to be extracted with lower specimen
The equipped facility of device, the present invention is provided with lid locating notch in the outward flange of capping.
Described lower specimen extraction assembly, as shown in Fig. 4 a-4f, including capillary and the branch for supporting capillary
Bearing portion part, and supporting member is blocked including bottom, the upper end that bottom is blocked has the lower flange alignd with capping outer rim, bottom
The lower end of blocking sets capillary pipe pig, and capillary is supported by capillary pipe pig；Lower flange is corresponding with upper lid locating notch
Position at be provided with bottom locating notch, while lower flange is symmetrically provided with two extraction assemblies separation breach；Bottom
Block and blocked with upper lid, open up thief hole, bottom Blind locating holes respectively at the corresponding position of upper lid fixed block, upper lid blocking is placed in
In thief hole, and upper lid fixed block is then placed in the Blind locating holes of bottom；Thief hole, the side wall of bottom Blind locating holes connect to form one
Through hole with positive stop lug boss.
Bottom on the outside of thief hole blocks and offers bottom dummy notch, and the bottom dummy notch is deep-slotted chip breaker, and
Caving in of having that shape is adapted to bottom Blind locating holes outer wall at the inner side cell wall position corresponding with bottom Blind locating holes is convex
Block, and deep-slotted chip breaker is with the Central Symmetry setting of bottom Blind locating holes.The purpose that deep-slotted chip breaker is set also is easy for the note of supporting member
Type is moulded into, the yield rate of product is improved.
The capillary pipe pig includes isosceles triangle cambered surface support and along isosceles triangle cambered surface support center line cloth
The capillary mounting rod put, the lower arcuate surface of the isosceles triangle cambered surface support is fixed on the lower surface of bottom blocking, and arc
The two ends of bottom surface are located at bottom and blocked on the center line of lower surface；Capillary is supported by capillary mounting rod.
At the upper end of supporting member and upper cover part snapping, upper lid blocks thief hole；Capillary passes through support
Part is configured at the inside of sample cut-back tank.
Meanwhile, when upper lid blocking is plugged in thief hole, placement above lower flange, and upper lid fixed block are close in capping
It is plugged in the Blind locating holes of bottom；Grafting, upper lid fixed block and the bottom that upper cover part is blocked with thief hole by upper lid are positioned
The snapping with supporting member is realized in the grafting of blind hole jointly.
In addition, sample cut-back tank of the present invention, as shown in Fig. 5 a-e, the sample cut-back tank includes diluter
Body, the diluter body has openend, and openend stretches out to form diluter flange, the openend of diluter body
By sealing aluminium foil sealing；Diluter body is provided with diluter seal groove, support in the inwall adjacent with diluter flange
The bottom of part blocks the inner wall sealing by the seal being installed in diluter seal groove and diluter body, and diluter sheet
The bottom of body is arranged to conical lower portion, while the outer wall of diluter body is provided with diluter outer wall anti-slip part, the anti-slip part is
The annular lines that a plurality of outer wall along diluter body is set.
According to above-mentioned technical scheme, it is known that quantitative sampling dilution device of the present invention has the following advantages that：
1st, the present invention creatively devises quantitative sampling and dilution evenly mixing device, is provided with and takes on lower specimen extraction assembly
Sample hole, sample, the problem of solving quantitative sample-adding are drawn for quantitative liquid-transfering gun.
2nd, for supporting the capillary pipe pig two ends of quantitative capillary tube in circle center line, it is ensured that can be accurate during insertion aluminium foil
Positioning plug above follow and be inserted into cut-back tank.Play quantitative capillary tube eccentric, and support is not eccentric.It is easy to behaviour
Make.
3rd, quantitative capillary tube device can accurately draw trace sample.Meanwhile, have and be easy to the design device for puncturing aluminium foil, have
Body is that quantitative capillary tube end is set into taper.
4th, there is alignment device between upper cover part and lower specimen extraction assembly（Upper lid locating notch, bottom locating slot
Mouthful）.There are the design-bottle opener and gap for being easy to corkage（Two extraction assemblies separate breach）.
According to above-mentioned quantitative sampling dilution device, a kind of quantitative sampling dilution process can be provided, comprised the following steps that：
（1）Sample dilution is dispensed and sample collection
Sample dilution is dispensed：Contained in sample cut-back tank quantitative（Such as 100-1500 microlitres）Sample dilution, and
Using sealing aluminium foil by the opening end closure of sample cut-back tank；
Sample collection：Using the capillary contact sample to be collected in sample amounts extraction assembly, capillary passes through capillary force
Effect quantify（8.5 microlitre）Suck sample to be collected；
（2）Sample Dilution is mixed first, and the capillary in sample amounts extraction assembly is puncturing sample cut-back tank openend
Seal after aluminium foil, continue to move ahead, until the lower flange that supporting member bottom is blocked in sample amounts extraction assembly compresses sample
The diluter flange of cut-back tank openend, completes the assembling of sample amounts extraction assembly and sample cut-back tank；Now, support
The bottom of part blocks the sealing in the diluter seal groove for passing through and being installed on and nestling up set by sample cut-back tank openend
The inner wall sealing of part and sample cut-back tank；Then, upper end and the sample dilution of sample amounts extraction assembly are pressed respectively
The bottom of container, turns upside down repeatedly（10 times）, until the sample in capillary and the Sample dilution in sample cut-back tank
Mix；
（3）Sample application
Upper cover part in sample amounts extraction assembly and lower specimen extraction device are separated, using quantitative liquid shifter by setting
The thief hole being placed on lower specimen extraction device is drawn quantitative（100 microlitres）Sample after dilution, is added after kit progress
Continuous biochemical reaction.
（4）Device is abandoned：By the upper lid locating notch of upper cover part and the bottom locating notch of bottom quantitative extractor part
After alignment, the upper lid of upper cover part is blocked and is plugged in thief hole, placement above lower flange is close in capping, and upper lid is solid
Determine block to be plugged in the Blind locating holes of bottom；Upper cover part blocks grafting, upper lid fixed block and the bottom with thief hole by upper lid
The snapping with supporting member is realized in the grafting of Blind locating holes jointly.
Claims (10)
1. a kind of quantitative sampling dilution device, including sample amounts extraction assembly and sample cut-back tank；Characterized in that, institute
Stating sample amounts extraction assembly includes upper cover part and lower specimen extraction device that split is set, lower specimen extraction device bag
Capillary and the supporting member for supporting capillary are included, the upper end of supporting member can be with upper cover part snapping, support
The lower end of part can be adapted to the openend of sample cut-back tank；The supporting member has the thief hole of insertion, upper cover part
Blocked with the upper lid that outer wall shape is adapted to thief hole inner wall shape；At the upper end of supporting member and upper cover part snapping,
Thief hole is blocked in upper lid blocking；Capillary is configured at the inside of sample cut-back tank by supporting member.
2. quantitative sampling dilution device according to claim 1, it is characterised in that the supporting member lower end sets capillary
Pipe pig；The capillary pipe pig includes isosceles triangle cambered surface support and arranged along isosceles triangle cambered surface support center line
Capillary mounting rod, the lower arcuate surface of the isosceles triangle cambered surface support is fixed on the lower surface of supporting member, and arcuate bottom
The two ends in face are located at bottom and blocked on the center line of lower surface, while thief hole is in the inner side of isosceles triangle cambered surface support；
Capillary is supported by capillary mounting rod.
3. quantitative sampling dilution device according to claim 2, it is characterised in that the supporting member includes outer wall shape
Blocked with sample cut-back tank close to the bottom that inner wall shape at aperture position is adapted to, thief hole prolongs along the length that bottom is blocked
Direction setting is stretched, capillary pipe pig is installed on the lower end of bottom blocking.
4. quantitative sampling dilution device according to claim 3, it is characterised in that the upper cover part includes capping, envelope
The upper end of lid is provided with hollow upper lid pressing chamber, and the lower end of capping is provided with described upper lid and blocked, and it is stifled to nestle up lid
Plug sets upper lid fixed block；The upper end that bottom is blocked has the lower flange alignd with capping outer rim, and bottom is blocked and upper lid
Inner wall shape and the bottom Blind locating holes of upper lid fixed block outer wall shape adaptation are set at the corresponding position of fixed block；It is stifled in upper lid
When plug is plugged in thief hole, placement above lower flange is close in capping, and upper lid fixed block is plugged in the Blind locating holes of bottom；
Upper cover part blocks the common realization of grafting, the grafting of upper lid fixed block and bottom Blind locating holes and branch with thief hole by upper lid
The snapping of bearing portion part.
5. quantitative sampling dilution device according to claim 4, it is characterised in that the outward flange of the capping is provided with
Lid locating notch, lower flange is provided with bottom locating notch at position corresponding with upper lid locating notch；While lower flange
There are two extraction assembly separation breach so that bottom locating notch is symmetrically disposed about.
6. quantitative sampling dilution device according to claim 5, it is characterised in that the section of the thief hole is circle,
The section of bottom Blind locating holes is prismatic, and thief hole, the side wall of bottom Blind locating holes, which are connected, to be formed one and have positive stop lug boss
Through hole.
7. quantitative sampling dilution device according to claim 6, it is characterised in that the bottom on the outside of thief hole is blocked
Bottom dummy notch is offered, the bottom dummy notch is deep-slotted chip breaker, and in the inner side cell wall corresponding with bottom Blind locating holes
There is the projection that caves in that shape is adapted to bottom Blind locating holes outer wall at position, and deep-slotted chip breaker is with the center pair of bottom Blind locating holes
Claim to set.
8. quantitative sampling dilution device according to claim 7, it is characterised in that the upper lid is blocked to be extended along cock body
Direction is provided with technique blind hole.
9. quantitative sampling dilution device according to claim 1, it is characterised in that the sample cut-back tank includes dilution
Device body, the diluter body has openend, and openend stretches out to form diluter flange, the opening of diluter body
End is by sealing aluminium foil sealing；Diluter body is provided with diluter seal groove in the inwall adjacent with diluter flange, supporting
The bottom of part blocks the inner wall sealing by the seal being installed in diluter seal groove and diluter body, and diluter
The bottom of body is arranged to conical lower portion, while the outer wall of diluter body is provided with diluter outer wall anti-slip part, the anti-slip part
The annular lines set for a plurality of outer wall along diluter body.
10. a kind of quantitative sampling dilution process, it is characterised in that comprise the following steps：
（1）Sample dilution is dispensed and sample collection
Sample dilution is dispensed：Quantitative Sample dilution is contained in sample cut-back tank, and uses sealing aluminium foil by sample
The opening end closure of cut-back tank；
Sample collection：Using the capillary contact sample to be collected in sample amounts extraction assembly, capillary passes through capillary force
Effect quantitatively suck sample to be collected；
（2）Sample Dilution is mixed
First, the capillary in sample amounts extraction assembly continues after the sealing aluminium foil of sample cut-back tank openend is punctured
Move ahead, until the lower flange that supporting member bottom is blocked in sample amounts extraction assembly compresses sample cut-back tank openend
Diluter flange, completes the assembling of sample amounts extraction assembly and sample cut-back tank；Now, the bottom of supporting member blocks logical
The seal crossed in the diluter seal groove for being installed on and nestling up set by sample cut-back tank openend and sample cut-back tank
Inner wall sealing；
Then, the upper end of sample amounts extraction assembly and the bottom of sample cut-back tank are pressed respectively, are turned upside down repeatedly,
Until the sample in capillary is mixed with the Sample dilution in sample cut-back tank；
（3）Sample application
Upper cover part in sample amounts extraction assembly and lower specimen extraction device are separated, using quantitative liquid shifter by setting
It is placed in the thief hole on lower specimen extraction device and draws the sample after quantitative dilution, adds the follow-up biochemistry of kit progress anti-
Should.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710377140.4A CN107192581B (en) | 2017-05-25 | 2017-05-25 | Quantitative sampling dilution device and method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710377140.4A CN107192581B (en) | 2017-05-25 | 2017-05-25 | Quantitative sampling dilution device and method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107192581A true CN107192581A (en) | 2017-09-22 |
| CN107192581B CN107192581B (en) | 2023-11-21 |
Family
ID=59875709
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710377140.4A Active CN107192581B (en) | 2017-05-25 | 2017-05-25 | Quantitative sampling dilution device and method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107192581B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111624085A (en) * | 2020-07-29 | 2020-09-04 | 天津中新科炬生物制药股份有限公司 | Sample diluting device and reagent card for detecting novel coronavirus antibody |
| CN112697515A (en) * | 2021-01-11 | 2021-04-23 | 安邦（厦门）生物科技有限公司 | Quantitative dilution sampling tube |
| CN113101985A (en) * | 2019-06-26 | 2021-07-13 | 京东方科技集团股份有限公司 | Detection chip and detection system |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5833630A (en) * | 1994-11-03 | 1998-11-10 | Kloth; Bernd | Sample collection device |
| CN1969184A (en) * | 2004-04-16 | 2007-05-23 | 拉希达·A·卡马利 | Specimen collecting, processing and analytical assembly |
| US20090209882A1 (en) * | 2008-02-15 | 2009-08-20 | Trinity Research Limited | Integrated cap and sample applicator |
| RU2010144027A (en) * | 2008-03-28 | 2012-05-10 | Орион Диагностика Ой (Fi) | DEVICE FOR SELECTION AND DOSING OF SAMPLES |
| CN102740978A (en) * | 2009-12-18 | 2012-10-17 | 扎芬纳股份公司 | Micropipette |
| CN104677689A (en) * | 2014-12-19 | 2015-06-03 | 江苏海王生物制药有限公司 | Suction device, sampling diluting device using suction device, analysis system and sampling analysis method |
| CN105452838A (en) * | 2013-07-26 | 2016-03-30 | 积水医疗株式会社 | Container for specimen preparation |
| CN206920171U (en) * | 2017-05-25 | 2018-01-23 | 南京岚煜生物科技有限公司 | quantitative sampling dilution device |
-
2017
- 2017-05-25 CN CN201710377140.4A patent/CN107192581B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5833630A (en) * | 1994-11-03 | 1998-11-10 | Kloth; Bernd | Sample collection device |
| CN1969184A (en) * | 2004-04-16 | 2007-05-23 | 拉希达·A·卡马利 | Specimen collecting, processing and analytical assembly |
| US20090209882A1 (en) * | 2008-02-15 | 2009-08-20 | Trinity Research Limited | Integrated cap and sample applicator |
| RU2010144027A (en) * | 2008-03-28 | 2012-05-10 | Орион Диагностика Ой (Fi) | DEVICE FOR SELECTION AND DOSING OF SAMPLES |
| CN102740978A (en) * | 2009-12-18 | 2012-10-17 | 扎芬纳股份公司 | Micropipette |
| CN105452838A (en) * | 2013-07-26 | 2016-03-30 | 积水医疗株式会社 | Container for specimen preparation |
| CN104677689A (en) * | 2014-12-19 | 2015-06-03 | 江苏海王生物制药有限公司 | Suction device, sampling diluting device using suction device, analysis system and sampling analysis method |
| CN206920171U (en) * | 2017-05-25 | 2018-01-23 | 南京岚煜生物科技有限公司 | quantitative sampling dilution device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113101985A (en) * | 2019-06-26 | 2021-07-13 | 京东方科技集团股份有限公司 | Detection chip and detection system |
| CN111624085A (en) * | 2020-07-29 | 2020-09-04 | 天津中新科炬生物制药股份有限公司 | Sample diluting device and reagent card for detecting novel coronavirus antibody |
| CN112697515A (en) * | 2021-01-11 | 2021-04-23 | 安邦（厦门）生物科技有限公司 | Quantitative dilution sampling tube |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107192581B (en) | 2023-11-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107192581A (en) | Quantitative sampling dilution device and its method | |
| US20040213699A1 (en) | Disposable apparatus for use in blood testing | |
| CN203954732U (en) | A kind of Multi-channel solid phase extraction device | |
| CN204405365U (en) | Sampler | |
| CN107561198A (en) | A kind of liquid chromatograph automatic sampler | |
| CN103889582A (en) | Centrifugal rotary disk | |
| CN206920171U (en) | quantitative sampling dilution device | |
| CN206657024U (en) | A kind of reagent pre-packaged cuvette | |
| CN107037176B (en) | The method and apparatus of sulfide content in a kind of detection methane gas | |
| CN205679577U (en) | A kind of chromatograph gas sample stablizes injector | |
| CN206248379U (en) | Portable micro sampling dilution Dropping feeder | |
| CN110082421A (en) | The laser ablation Plasma-Mass Spectroscopy constituent analysis technology of ultra micro volume of liquid | |
| US9383298B2 (en) | Method for preparing a sample for analysis | |
| CN215140039U (en) | Porous quantitative sample injector | |
| CN207587693U (en) | A kind of accurate sampling of isotope mass spectrometer and sampling device | |
| CN104155146B (en) | A kind of portable ore pulp sampling device and method thereof | |
| CN209061162U (en) | Test tube | |
| CN221899124U (en) | Pigment detects pretreatment ration, filtration, enrichment facility | |
| CN220829465U (en) | Ammonia nitrogen detection kit | |
| CN206399697U (en) | One kind delay point liquid sampler | |
| CN207976324U (en) | A kind of Experiment of Analytical Chemistry gas sampling assembly | |
| CN110296870A (en) | A kind of low melting point chemical product detection sampler | |
| CN220590070U (en) | Laboratory no-pipeline drip | |
| CN209254791U (en) | A kind of drug detection liquid quantitative pipettor | |
| CN220188178U (en) | Trace sample digestion bottle |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 211100 room 214, building 9, No. 2, Qiande Road, high tech Zone, Jiangning District, Nanjing, Jiangsu Province Applicant after: Nanjing lanxuan Biotechnology Co.,Ltd. Address before: 215000 3, 1 building, 8 Ling Ling Road, hi tech Zone, Suzhou, Jiangsu. Applicant before: NANJING LANSION BIOTECHNOLOGY Co.,Ltd. |
|
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: Room 1004, floor 10, Block E, phase II, Dingye Baitai biological building, No. 10, Xinghuo Road, Jiangbei new area, Nanjing, Jiangsu 210000 Applicant after: Jiangsu Huakong Biotechnology Co.,Ltd. Address before: 211100 room 214, building 9, No. 2, Qiande Road, high tech Zone, Jiangning District, Nanjing, Jiangsu Province Applicant before: Nanjing lanxuan Biotechnology Co.,Ltd. |
|
| CB02 | Change of applicant information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |