CN107192581B - Quantitative sampling dilution device and method thereof - Google Patents
Quantitative sampling dilution device and method thereof Download PDFInfo
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- CN107192581B CN107192581B CN201710377140.4A CN201710377140A CN107192581B CN 107192581 B CN107192581 B CN 107192581B CN 201710377140 A CN201710377140 A CN 201710377140A CN 107192581 B CN107192581 B CN 107192581B
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- 238000005070 sampling Methods 0.000 title claims abstract description 66
- 239000012895 dilution Substances 0.000 title claims abstract description 24
- 238000010790 dilution Methods 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000000605 extraction Methods 0.000 claims abstract description 61
- 239000012898 sample dilution Substances 0.000 claims abstract description 52
- 238000005842 biochemical reaction Methods 0.000 claims abstract description 4
- 239000000523 sample Substances 0.000 claims description 103
- 238000007789 sealing Methods 0.000 claims description 34
- 238000007865 diluting Methods 0.000 claims description 15
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 13
- 229910052782 aluminium Inorganic materials 0.000 claims description 13
- 239000011888 foil Substances 0.000 claims description 13
- 239000003085 diluting agent Substances 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 7
- 238000003825 pressing Methods 0.000 claims description 7
- 239000012470 diluted sample Substances 0.000 claims description 6
- 238000003113 dilution method Methods 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- 238000013461 design Methods 0.000 description 4
- 230000000903 blocking effect Effects 0.000 description 2
- 238000001746 injection moulding Methods 0.000 description 2
- 238000012123 point-of-care testing Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000012631 diagnostic technique Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
- G01N2001/1472—Devices not actuated by pressure difference
- G01N2001/149—Capillaries; Sponges
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
- G01N2001/386—Other diluting or mixing processes
Abstract
The invention discloses a quantitative sampling dilution device and a method thereof, wherein the device comprises a sample quantitative extraction component and a sample dilution container; the sample quantitative extraction assembly comprises an upper cover part and a lower sample extraction device which are arranged in a split manner, wherein the lower sample extraction device comprises a capillary tube and a supporting part for supporting the capillary tube, the upper end of the supporting part can be buckled with the upper cover part, and the lower end of the supporting part can be matched with the opening end of the sample dilution container; the supporting component is provided with a through sampling hole, and the upper cover part is provided with an upper cover plug with an outer wall shape matched with the inner wall shape of the sampling hole; when the upper end of the supporting component is buckled with the upper cover part, the upper cover blocks the sampling hole; the capillary tube is disposed inside the sample dilution container via the support member. Therefore, the invention not only can realize quantitative sample dilution, but also can finish quantitative sample transferring to the kit for biochemical reaction.
Description
Technical Field
The invention relates to a quantitative sampling dilution device and a quantitative sampling dilution method, which are used for quantitative sampling dilution and uniform mixing of a trace sample.
Background
The immunolateral chromatography diagnostic technique is suitable for use in a variety of point of care testing (POCT) or on site as a stable and practical technique.
The existing quantitative sampling device samples, dilutes and mixes the back, drips from the drip outlet above, this kind of design is in the test of the design product because its simplicity is advantageous. However, the quantitative product loading brings about a great deal of uncertainty to the quantitative product due to the risk of more or less than one drop. Meanwhile, bubbles are generated in the process of uniformly mixing the liquid in an up-and-down rotating way, so that the first drop of liquid can contain inaccuracy of the bubble adding amount in the dropping process.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a quantitative sampling dilution device which can realize accurate sampling through a capillary vessel arranged on the device, and can accurately sample through a sample adding hole arranged on the device, and a pipetting gun which absorbs a fixed amount is adopted, so that the uncertainty of direct liquid dropping is avoided.
In order to achieve the technical purpose, the invention adopts the following technical scheme:
a quantitative sampling dilution device comprises a sample quantitative extraction component and a sample dilution container; the quantitative sample extraction assembly comprises an upper cover part and a lower sample extraction device which are arranged in a split manner, wherein the lower sample extraction device comprises a capillary tube and a supporting part for supporting the capillary tube, the upper end of the supporting part can be buckled with the upper cover part, and the lower end of the supporting part can be matched with the opening end of the sample dilution container; the supporting component is provided with a through sampling hole, and the upper cover part is provided with an upper cover plug with an outer wall shape matched with the inner wall shape of the sampling hole; when the upper end of the supporting component is buckled with the upper cover part, the upper cover blocks the sampling hole; the capillary tube is disposed inside the sample dilution container via the support member.
The lower end of the supporting part is provided with a capillary bracket; the capillary support comprises isosceles triangle cambered surface brackets and capillary mounting rods which are arranged along the central line of the isosceles triangle cambered surface brackets, the arc bottom surfaces of the isosceles triangle cambered surface brackets are fixed on the lower end surface of the supporting part, two ends of the arc bottom surfaces are positioned on the central line of the lower end surface of the lower part of the supporting part, and the sampling holes are positioned on the inner side of the isosceles triangle cambered surface brackets; the capillary tube is supported by a capillary tube mounting stem.
The support member comprises a lower plug having an outer wall shaped to conform to the shape of the inner wall of the sample dilution container immediately adjacent the opening, the sampling hole is arranged along the length extending direction of the lower plug, and the capillary bracket is arranged at the lower end of the lower plug.
The upper cover part comprises a sealing cover, a hollow upper cover pressing cavity is arranged at the upper end of the sealing cover, the lower end of the sealing cover is provided with an upper cover plug, and an upper cover fixing block is arranged close to the upper cover plug; the upper end of the lower plug is provided with a lower flange aligned with the outer edge of the sealing cover, and a lower positioning blind hole with an inner wall shape matched with the outer wall shape of the upper cover fixing block is arranged at the position of the lower plug corresponding to the upper cover fixing block; when the upper cover is plugged in the sampling hole, the sealing cover is placed closely above the lower flange, and the upper cover fixing block is plugged in the lower positioning blind hole; the upper cover part is plugged with the sampling hole through the upper cover, and the upper cover fixing block is plugged with the lower positioning blind hole to jointly realize the buckling connection with the supporting part.
The outer edge of the sealing cover is provided with an upper cover positioning notch, and a lower flange is provided with a lower positioning notch at a position corresponding to the upper cover positioning notch; simultaneously, the lower flange is symmetrically provided with two extraction component separation notches by taking the lower positioning notch as a center.
The section of the sampling hole is circular, the section of the lower positioning blind hole is prismatic, and the side walls of the sampling hole and the lower positioning blind hole are communicated to form a through hole with a limiting boss.
The lower process notch is an arc-shaped groove and is provided with an inward sinking lug with a shape matched with the outer wall of the lower positioning blind hole at the position of the inner side groove wall corresponding to the lower positioning blind hole, and the arc-shaped groove is symmetrically arranged with the center of the lower positioning blind hole.
The upper cover plug is provided with a process blind hole along the extending direction of the plug body.
The sample dilution container comprises a diluter body, wherein the diluter body is provided with an opening end, the opening end extends outwards to form a diluter flange, and the opening end of the diluter body is sealed by a sealing aluminum foil; the diluter body is provided with the diluter seal groove at the inner wall adjacent with the diluter flange, and the lower part jam of supporting part is sealed with the inner wall of diluter body through the sealing member of installing in the diluter seal groove, and the bottom of diluter body sets up into the toper bottom, and the outer wall of diluter body is provided with diluter outer wall antiskid portion simultaneously, and this antiskid is many annular lines that set up along the outer wall of diluter body.
Another technical object of the present invention is to provide a quantitative sampling dilution method, comprising the steps of:
(1) Sample diluent split charging and sample collection
Sample diluent split charging: a certain amount of sample diluent is contained in the sample dilution container, and the opening end of the sample dilution container is sealed by adopting a sealing aluminum foil;
sample collection: contacting a capillary tube in the sample quantitative extraction assembly with a sample to be collected, and quantitatively sucking the capillary tube into the sample to be collected by the action of capillary force;
(2) The sample dilution and mixing are carried out uniformly, firstly, a capillary tube in the sample quantitative extraction assembly continuously moves forward after a sealing aluminum foil at the opening end of a sample dilution container is pierced, until a lower flange blocked by the lower part of a supporting part in the sample quantitative extraction assembly presses a diluter flange at the opening end of the sample dilution container, so that the assembly of the sample quantitative extraction assembly and the sample dilution container is completed; at this time, the lower plug of the supporting member is sealed with the inner wall of the sample dilution container by a seal fitted in a seal groove of the diluter provided in close proximity to the open end of the sample dilution container; then, pressing the upper end of the sample quantitative extraction component and the bottom of the sample diluting container respectively, and reversing the operation for a plurality of times until the sample in the capillary tube is uniformly mixed with the sample diluting liquid in the sample diluting container;
(3) Sample addition
And separating an upper cover part from a lower sample extraction device in the sample quantitative extraction assembly, sucking a quantitatively diluted sample through a sampling hole arranged on the lower sample extraction device by adopting a quantitative pipette, and adding the quantitatively diluted sample into a kit to perform subsequent biochemical reaction.
According to the technical scheme, compared with the prior art, the invention has the following beneficial effects:
in immune layer reaction systems, especially in quantitative products, the accuracy of the sample addition plays an important role, and microsampling (below 20 microliters) has high requirements on the sample addition device and high maintenance frequency. The invention quantitatively takes the sample with micro volume through the capillary vessel, and performs dilution and uniform mixing in the bottle body, thereby achieving the purpose of accurate sampling. Meanwhile, a fixed amount of liquid is sucked by the pipette to carry out sample adding operation, so that the uncertainty of direct liquid dropping is avoided. Direct dripping risks the wrong number of drops, and the first drop may have bubbles, causing inaccuracy in the application of the sample.
Drawings
FIG. 1a is a front view of a quantitative sampling dilution apparatus according to the present invention;
FIG. 1b is a top view of a quantitative sampling dilution apparatus according to the present invention;
FIG. 1c is a bottom view of the quantitative sampling dilution apparatus of the present invention;
in fig. 1a, 1b, 1 c: 1. an upper cover part; 2. a lower sample extraction device; 3. a sample dilution vessel;
FIG. 2 is an exploded view of the quantitative sampling dilution apparatus of the present invention;
in fig. 2: 1. an upper cover part; 2. a lower sample extraction device; 3. a sample dilution vessel; 4. sealing aluminum foil;
FIG. 3a is a schematic perspective view of the upper cover part of the quantitative sample extraction assembly according to the present invention;
FIG. 3b is a semi-sectional view of FIG. 3 a;
FIG. 3c is a front view of the upper cover portion;
FIG. 3d is a top view of the upper cover portion;
FIG. 3e is a bottom view of the upper cover portion;
in fig. 3a-3 e: 1-1, an upper cover pressing cavity; 1-2, sealing; 1-3, positioning notch of upper cover; 1-4, blocking an upper cover; 1-5, an upper cover fixing block; 1-6, technological blind holes;
FIG. 4a is a schematic perspective view of a lower sample extraction device in the sample quantification extraction assembly of the present invention;
FIG. 4b is a semi-sectional view of the lower sample extraction device of FIG. 3 a;
FIG. 4c is a top view of the lower sample extraction device;
FIG. 4d is a bottom view of the lower sample extraction device;
FIG. 4e is a front view of FIG. 4 c;
FIG. 4f is a right side view of FIG. 4 d;
in fig. 4a-4 f: 2-1, a lower flange; 2-2, lower part blocking; 2-3, separating gaps of the extraction component; 2-4, a lower positioning notch; 2-5, a lower process notch; 2-6, sampling holes; 2-7, a lower positioning blind hole; 2-8, quantifying the capillary; 2-9, capillary support;
FIG. 5a is a schematic perspective view of a sample dilution vessel according to the invention;
FIG. 5b is a semi-sectional view of FIG. 5 a;
FIG. 5c is a front view of the sample dilution vessel;
FIG. 5d is a top view of the sample dilution vessel;
FIG. 5e is a bottom view of the sample dilution container;
in fig. 5a-5 e: 3-1, diluter flanges; 3-2, a diluter body; 3-3, an outer wall anti-slip part of the diluter; 3-4, sealing the groove of the diluter; 3-5, the conical bottom of the diluter.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. The following description of at least one exemplary embodiment is merely exemplary in nature and is in no way intended to limit the invention, its application, or uses. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention. The relative arrangement, expressions and numerical values of the components and steps set forth in these embodiments do not limit the scope of the present invention unless it is specifically stated otherwise. Meanwhile, it should be understood that the sizes of the respective parts shown in the drawings are not drawn in actual scale for convenience of description. Techniques, methods, and apparatus known to one of ordinary skill in the relevant art may not be discussed in detail, but should be considered part of the specification where appropriate. In all examples shown and discussed herein, any specific values should be construed as merely illustrative, and not a limitation. Thus, other examples of the exemplary embodiments may have different values.
Spatially relative terms, such as "above … …," "above … …," "upper surface at … …," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial location relative to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations in use or operation in addition to the orientation depicted in the figures. For example, if the device in the figures is turned over, elements described as "above" or "over" other devices or structures would then be oriented "below" or "beneath" the other devices or structures. Thus, the exemplary term "above … …" may include both orientations of "above … …" and "below … …". The device may also be positioned in other different ways (rotated 90 degrees or at other orientations).
As shown in fig. 1a, 1b, 1c and fig. 2, the quantitative sampling dilution device according to the present invention includes two parts, namely a quantitative sample extraction assembly and a sample dilution container; wherein: the sample quantitative extraction component and the sample dilution container can be produced respectively in production practice or can be produced in a matched manner; however, when in use, the two components are matched to realize dilution of biological samples.
Specifically, in order to realize quantitative sampling of sample dilution and quantitative sample adding during sample detection, the sample quantitative extraction component provided by the invention is divided into an upper part and a lower part, and is correspondingly provided with an upper cover part and a lower sample extraction device, wherein:
the upper cover part, as shown in figures 3a-e, comprises a sealing cover, wherein the upper end of the sealing cover is provided with a hollow upper cover pressing cavity, the lower end of the sealing cover is provided with an upper cover plug, and an upper cover fixing block is arranged close to the upper cover plug; in addition, in order to facilitate the injection molding of the product, the upper cover plug is provided with a process blind hole along the extending direction of the plug body; and the invention is provided with an upper cover positioning notch at the outer edge of the cover, thereby facilitating the assembly of the upper cover part and the lower sample extraction device.
The lower sample extraction assembly, as shown in fig. 4a-4f, comprises a capillary tube and a support member for supporting the capillary tube, wherein the support member comprises a lower plug, the upper end of the lower plug is provided with a lower flange aligned with the outer edge of the cover, the lower end of the lower plug is provided with a capillary bracket, and the capillary tube is supported by the capillary bracket; the lower flange is provided with a lower positioning notch at a position corresponding to the upper cover positioning notch, and two extraction component separation notches are symmetrically arranged on the lower flange; the lower part plug is provided with a sampling hole and a lower part positioning blind hole at the positions corresponding to the upper cover plug and the upper cover fixing block respectively, the upper cover plug is arranged in the sampling hole, and the upper cover fixing block is arranged in the lower part positioning blind hole; the side walls of the sampling hole and the lower positioning blind hole are communicated to form a through hole with a limiting boss.
The lower process notch is an arc-shaped groove and is provided with an inward sinking lug with a shape matched with the outer wall of the lower positioning blind hole at the position of the inner side groove wall corresponding to the lower positioning blind hole, and the arc-shaped groove is symmetrically arranged with the center of the lower positioning blind hole. The arc-shaped groove is also convenient for injection molding of the supporting part, and the yield of products is improved.
The capillary support comprises isosceles triangle cambered surface brackets and capillary mounting rods which are arranged along the central line of the isosceles triangle cambered surface brackets, wherein the arc bottom surfaces of the isosceles triangle cambered surface brackets are fixed on the lower end surface of the lower plug, and the two ends of the arc bottom surfaces are positioned on the central line of the lower end surface of the lower plug; the capillary tube is supported by a capillary tube mounting stem.
When the upper end of the supporting component is buckled with the upper cover part, the upper cover blocks the sampling hole; the capillary tube is disposed inside the sample dilution container via the support member.
Meanwhile, when the upper cover is plugged into the sampling hole, the sealing cover is placed closely above the lower flange, and the upper cover fixing block is plugged into the lower positioning blind hole; the upper cover part is plugged with the sampling hole through the upper cover, and the upper cover fixing block is plugged with the lower positioning blind hole to jointly realize the buckling connection with the supporting part.
In addition, the sample diluting container according to the present invention, as shown in fig. 5a-e, comprises a diluter body having an open end, the open end extending outwardly to form a diluter flange, the open end of the diluter body being sealed by a sealing aluminum foil; the diluter body is provided with the diluter seal groove at the inner wall adjacent with the diluter flange, and the lower part jam of supporting part is sealed with the inner wall of diluter body through the sealing member of installing in the diluter seal groove, and the bottom of diluter body sets up into the toper bottom, and the outer wall of diluter body is provided with diluter outer wall antiskid portion simultaneously, and this antiskid is many annular lines that set up along the outer wall of diluter body.
According to the technical scheme, the quantitative sampling dilution device has the following advantages:
1. the invention creatively designs a quantitative sampling and diluting mixing device, and a sampling hole is arranged on a lower sample extraction component and is used for sucking a sample by a quantitative pipette, so that the problem of quantitative sample feeding is solved.
2. The two ends of the capillary support for supporting the quantitative capillary are arranged on the circular center line, so that the plug on the upper surface can be accurately positioned when the aluminum foil is inserted, and then the plug is inserted into the dilution container. The quantitative capillary tube is eccentric, and the bracket is not eccentric. Easy to operate.
3. The quantitative capillary device can accurately absorb trace samples. Meanwhile, there are design devices that are easy to pierce the aluminum foil, specifically, the end of the dosing capillary is tapered.
4. Alignment means (upper cap locating notch, lower locating notch) are provided between the upper cap portion and the lower sample extraction assembly. There is an easy-to-open design-bottle opener and opening (two extraction assembly separation gap).
According to the quantitative sampling dilution device, a quantitative sampling dilution method can be provided, and the specific steps are as follows:
(1) Sample diluent split charging and sample collection
Sample diluent split charging: a sample diluting container is filled with a certain amount (such as 100-1500 microliters) of sample diluting liquid, and an opening end of the sample diluting container is sealed by adopting a sealing aluminum foil;
sample collection: contacting a capillary in the sample quantitative extraction component with a sample to be collected, and quantitatively (8.5 microliters) sucking the capillary into the sample to be collected by the action of capillary force;
(2) The sample dilution and mixing are carried out uniformly, firstly, a capillary tube in the sample quantitative extraction assembly continuously moves forward after a sealing aluminum foil at the opening end of a sample dilution container is pierced, until a lower flange blocked by the lower part of a supporting part in the sample quantitative extraction assembly presses a diluter flange at the opening end of the sample dilution container, so that the assembly of the sample quantitative extraction assembly and the sample dilution container is completed; at this time, the lower plug of the supporting member is sealed with the inner wall of the sample dilution container by a seal fitted in a seal groove of the diluter provided in close proximity to the open end of the sample dilution container; then, pressing the upper end of the quantitative sample extraction component and the bottom of the sample diluting container respectively, and reversing the operation for a plurality of times (10 times) until the sample in the capillary tube and the sample diluting liquid in the sample diluting container are uniformly mixed;
(3) Sample addition
And separating an upper cover part from a lower sample extraction device in the sample quantitative extraction assembly, sucking a quantitative (100 microliters) diluted sample through a sampling hole arranged on the lower sample extraction device by adopting a quantitative pipette, and adding the diluted sample into a kit to perform subsequent biochemical reaction.
(4) The device is abandoned: after aligning the upper cover positioning notch of the upper cover part with the lower positioning notch of the lower quantitative extraction device, plugging the upper cover of the upper cover part into the sampling hole, placing the sealing cover closely above the lower flange, and plugging the upper cover fixing block into the lower positioning blind hole; the upper cover part is plugged with the sampling hole through the upper cover, and the upper cover fixing block is plugged with the lower positioning blind hole to jointly realize the buckling connection with the supporting part.
Claims (8)
1. A quantitative sampling dilution device comprises a sample quantitative extraction component and a sample dilution container; the quantitative sample extraction assembly is characterized by comprising an upper cover part and a lower sample extraction device which are arranged in a split manner, wherein the lower sample extraction device comprises a capillary tube and a supporting part for supporting the capillary tube, the upper end of the supporting part can be buckled with the upper cover part, and the lower end of the supporting part can be matched with the opening end of the sample dilution container; the supporting component is provided with a through sampling hole, and the upper cover part is provided with an upper cover plug with an outer wall shape matched with the inner wall shape of the sampling hole; when the upper end of the supporting component is buckled with the upper cover part, the upper cover blocks the sampling hole; the capillary is arranged in the sample dilution container through the supporting component;
the supporting part comprises a lower part plug with an outer wall shape matched with the inner wall shape of the sample dilution container at the position close to the opening, and the sampling hole is arranged along the length extending direction of the lower part plug;
the upper cover part comprises a sealing cover, a hollow upper cover pressing cavity is arranged at the upper end of the sealing cover, the lower end of the sealing cover is provided with an upper cover plug, and an upper cover fixing block is arranged close to the upper cover plug; the upper end of the lower plug is provided with a lower flange aligned with the outer edge of the sealing cover, and a lower positioning blind hole with an inner wall shape matched with the outer wall shape of the upper cover fixing block is arranged at the position of the lower plug corresponding to the upper cover fixing block; when the upper cover is plugged in the sampling hole, the sealing cover is placed closely above the lower flange, and the upper cover fixing block is plugged in the lower positioning blind hole;
the upper cover part is plugged with the sampling hole through the upper cover, and the upper cover fixing block is plugged with the lower positioning blind hole to jointly realize the buckling connection with the supporting part;
the sample dilution container comprises a diluter body, wherein the diluter body is provided with an opening end, the opening end extends outwards to form a diluter flange, the opening end of the diluter body is sealed by a sealing aluminum foil, and a fixed amount of sample diluent is packaged in the sample dilution container; the inner wall of the diluter body adjacent to the diluter flange is provided with a diluter sealing groove, the lower part of the supporting part is blocked and sealed with the inner wall of the diluter body through a sealing piece arranged in the diluter sealing groove, the bottom of the diluter body is provided with a conical bottom, meanwhile, the outer wall of the diluter body is provided with a diluter outer wall anti-skid part, and the anti-skid part is a plurality of annular lines arranged along the outer wall of the diluter body;
after the capillary tube in the sample quantitative extraction assembly punctures the sealing aluminum foil at the opening end of the sample dilution container, continuing to move forward until the lower flange blocked by the lower part of the supporting part in the sample quantitative extraction assembly presses the diluter flange at the opening end of the sample dilution container, so that the assembly of the sample quantitative extraction assembly and the sample dilution container is completed; at this time, the lower plug of the support member is sealed with the inner wall of the sample dilution container by a seal fitted in a seal groove of the diluter provided immediately adjacent to the open end of the sample dilution container.
2. The quantitative sampling diluting apparatus according to claim 1, wherein a capillary bracket is provided at a lower end of the supporting member; the capillary support comprises isosceles triangle cambered surface brackets and capillary mounting rods which are arranged along the central line of the isosceles triangle cambered surface brackets, the arc bottom surfaces of the isosceles triangle cambered surface brackets are fixed on the lower end surface of the supporting part, two ends of the arc bottom surfaces are positioned on the central line of the lower end surface of the lower part of the supporting part, and the sampling holes are positioned on the inner side of the isosceles triangle cambered surface brackets;
the capillary tube is supported by a capillary tube mounting stem.
3. A quantitative sampling dilution apparatus according to claim 2, wherein the capillary bracket is mounted at a lower end of the lower plug.
4. A quantitative sampling dilution apparatus according to claim 3, wherein an outer edge of the cover is provided with an upper cover positioning notch, and a lower flange is provided with a lower positioning notch at a position corresponding to the upper cover positioning notch; simultaneously, the lower flange is symmetrically provided with two extraction component separation notches by taking the lower positioning notch as a center.
5. The quantitative sampling dilution device according to claim 4, wherein the cross section of the sampling hole is circular, the cross section of the lower positioning blind hole is prismatic, and the side walls of the sampling hole and the lower positioning blind hole are communicated to form a through hole with a limiting boss.
6. The quantitative sampling dilution apparatus according to claim 5, wherein a lower process notch is provided on a lower plug outside the sampling hole, the lower process notch is an arc-shaped groove, and an inward-sinking bump having a shape adapted to an outer wall of the lower positioning blind hole is provided at a position of an inner groove wall corresponding to the lower positioning blind hole, and the arc-shaped groove is symmetrically provided with a center of the lower positioning blind hole.
7. The quantitative sampling dilution apparatus according to claim 6, wherein the upper cover plug is provided with a blind process hole along an extension direction of the plug body.
8. A quantitative sampling dilution method based on the quantitative sampling dilution apparatus according to claim 1, comprising the steps of:
(1) Sample diluent split charging and sample collection
Sample diluent split charging: a certain amount of sample diluent is contained in the sample dilution container, and the opening end of the sample dilution container is sealed by adopting a sealing aluminum foil;
sample collection: contacting a capillary tube in the sample quantitative extraction assembly with a sample to be collected, and quantitatively sucking the capillary tube into the sample to be collected by the action of capillary force;
(2) Sample dilution and mixing
Firstly, after a capillary tube in the sample quantitative extraction assembly punctures a sealing aluminum foil at the opening end of a sample dilution container, continuing to move forward until a lower flange blocked by the lower part of a supporting part in the sample quantitative extraction assembly presses a diluter flange at the opening end of the sample dilution container, so that the assembly of the sample quantitative extraction assembly and the sample dilution container is completed; at this time, the lower plug of the supporting member is sealed with the inner wall of the sample dilution container by a seal fitted in a seal groove of the diluter provided in close proximity to the open end of the sample dilution container;
then, pressing the upper end of the sample quantitative extraction component and the bottom of the sample diluting container respectively, and reversing the operation for a plurality of times until the sample in the capillary tube is uniformly mixed with the sample diluting liquid in the sample diluting container;
(3) Sample addition
And separating an upper cover part from a lower sample extraction device in the sample quantitative extraction assembly, sucking a quantitatively diluted sample through a sampling hole arranged on the lower sample extraction device by adopting a quantitative pipette, and adding the quantitatively diluted sample into a kit to perform subsequent biochemical reaction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710377140.4A CN107192581B (en) | 2017-05-25 | 2017-05-25 | Quantitative sampling dilution device and method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710377140.4A CN107192581B (en) | 2017-05-25 | 2017-05-25 | Quantitative sampling dilution device and method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107192581A CN107192581A (en) | 2017-09-22 |
| CN107192581B true CN107192581B (en) | 2023-11-21 |
Family
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| CN113101985B (en) * | 2019-06-26 | 2022-07-22 | 京东方科技集团股份有限公司 | Detection chip and detection system |
| CN111624085B (en) * | 2020-07-29 | 2020-10-23 | 天津中新科炬生物制药股份有限公司 | Sample diluting device and reagent card for detecting novel coronavirus antibody |
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| US5833630A (en) * | 1994-11-03 | 1998-11-10 | Kloth; Bernd | Sample collection device |
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| RU2010144027A (en) * | 2008-03-28 | 2012-05-10 | Орион Диагностика Ой (Fi) | DEVICE FOR SELECTION AND DOSING OF SAMPLES |
| CN102740978A (en) * | 2009-12-18 | 2012-10-17 | 扎芬纳股份公司 | Micropipette |
| CN104677689A (en) * | 2014-12-19 | 2015-06-03 | 江苏海王生物制药有限公司 | Suction device, sampling diluting device using suction device, analysis system and sampling analysis method |
| CN105452838A (en) * | 2013-07-26 | 2016-03-30 | 积水医疗株式会社 | Container for specimen preparation |
| CN206920171U (en) * | 2017-05-25 | 2018-01-23 | 南京岚煜生物科技有限公司 | quantitative sampling dilution device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20090209882A1 (en) * | 2008-02-15 | 2009-08-20 | Trinity Research Limited | Integrated cap and sample applicator |
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| US5833630A (en) * | 1994-11-03 | 1998-11-10 | Kloth; Bernd | Sample collection device |
| CN1969184A (en) * | 2004-04-16 | 2007-05-23 | 拉希达·A·卡马利 | Specimen collecting, processing and analytical assembly |
| RU2010144027A (en) * | 2008-03-28 | 2012-05-10 | Орион Диагностика Ой (Fi) | DEVICE FOR SELECTION AND DOSING OF SAMPLES |
| CN102740978A (en) * | 2009-12-18 | 2012-10-17 | 扎芬纳股份公司 | Micropipette |
| CN105452838A (en) * | 2013-07-26 | 2016-03-30 | 积水医疗株式会社 | Container for specimen preparation |
| CN104677689A (en) * | 2014-12-19 | 2015-06-03 | 江苏海王生物制药有限公司 | Suction device, sampling diluting device using suction device, analysis system and sampling analysis method |
| CN206920171U (en) * | 2017-05-25 | 2018-01-23 | 南京岚煜生物科技有限公司 | quantitative sampling dilution device |
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