CN107185850A - A kind of Vigor of Zea mays L. Seeds detection means based on high light spectrum image-forming and electrical impedance - Google Patents
A kind of Vigor of Zea mays L. Seeds detection means based on high light spectrum image-forming and electrical impedance Download PDFInfo
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- CN107185850A CN107185850A CN201710425116.3A CN201710425116A CN107185850A CN 107185850 A CN107185850 A CN 107185850A CN 201710425116 A CN201710425116 A CN 201710425116A CN 107185850 A CN107185850 A CN 107185850A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
- B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
- B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
- B07C5/34—Sorting according to other particular properties
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Abstract
The present invention discloses a kind of Vigor of Zea mays L. Seeds detection means based on high light spectrum image-forming and electrical impedance, including：The magazine of corn seed to be measured is stored, the bottom of the magazine is connected with one or more single seeded chutes of output；It is provided with the conveyer belt of detection plate, detection hole of the detection plate provided with the seed for receiving chute output, each detection hole and is provided with electrode pair；The electric impedance analyzer being connected with electrode pair, for applying exciting current to seed by electrode, according to the electrical impedance data between electrode pair；Hyperspectral imaging devices above conveyer belt, for carrying out real time imagery to the corn seed in detection hole；Computer, for carrying out comprehensive analysis to electrical impedance data and high-spectral data, is classified to Vigor of Zea mays L. Seeds；Collection vessel positioned at conveyer belt one end, is inside provided with least two holding tanks, the corn seed for receiving different motility grades.The present invention has the advantages that lossless, automaticity is high, detection is quick, screening is efficient.
Description
Technical field
The present invention relates to the field of vegetable seeds viability examination.It is specifically related to a kind of based on high light spectrum image-forming and electrical impedance
Vigor of Zea mays L. Seeds detection means.
Background technology
Corn is China the first generalized grain crop, and the quality of corn seed is directly connected to benefit and the peasant of Seed enterprises
Income.In annual seed quality dispute, the germination percentage and Seedling emergence rate gap for frequently encountering laboratory are very big, indoor
Germination percentage meets label for labelling value and Seedling emergence rate is difficult to situation up to standard, here it is the problem of seed vitality is caused.Seed
Vigor is the robustness of seed, is germination and emergence rate, the potentiality of growth of seedling, plant anti-adversity ability and productive potentialities
Summation, is the important indicator of seed quality.Viability examination is carried out to seed before planting to become as an essential ring, it is right
Agricultural production plays vital effect.
Testing Seed Vigor can be divided into electrophysiological assays and biochemical assay.Electrophysiological assays are measurement germination sides
The characteristic and growth indexes in face；Biochemical assay is the measurement special biochemical reaction related to seed vitality, such as enzyme system activity
Deng.Currently, traditional assay method on Vigor of Zea mays L. Seeds mainly has：Anti- cold measurement method, growth of seedling determination method, acceleration are old
Change test method(s), conductimetric method etc..It is big, repeatable to there is measurement workload in above-mentioned traditional seed vitality detection method
Difference, measurement period length, it is affected by environment it is big, the shortcomings of easily cause damage to seed.
High light spectrum image-forming technology is collection detector technology, precision optics machinery, Detection of Weak Signals, computer technology, letter
Cease treatment technology in the integrated technology of one, with more than wave band and narrow width, spectral response range be wide, spectral resolution is high and
The features such as " spectrum is as unification ".In ultraviolet, visible ray, near-infrared and mid infrared region, obtain hundreds of meter wave band intervals it is very small and
The continuous spectroscopic data of spectrum.Each pixel in spectrum corresponds to a continuous spectrum, can reflect the inside of determinand
Composition constitutes information, is had broad application prospects in agricultural research field.
Seed has the attribute of absorption to light, and in the case where beam intensity difference is different with light beam closeness, seed
Surface is reflected and body reflection is different.And this optical characteristics of seed be it is relevant with Interior Seed physical signs, therefore
Non-Destructive Testing can be carried out to the vigor of seed using the optical characteristics of seed by high light spectrum image-forming technology.
On the other hand, seed moisture content and seed vitality are closely related.When water content is high, the respiration of seed adds
By force, the consumption of reserve substance accelerates in seed, the viability decline of seed.Organic matter accumulation is few, and enzymatic activity is low, it is impossible to meets and plants
Son sprouts required energy, causes germination percentage low.On the other hand, water content is too low to cause respiration excessively weak, seed vitality
Also it can reduce.Therefore, suitable water content is the important indicator of seed vitality height, also may be used by the measure to seed moisture content
To reflect seed vitality to a certain extent.
In order to overcome the shortcoming of existing technologies, the present invention is different from traditional electrophysiological assays and biochemical assay, passes through height
Spectral imaging technology and electrical impedance technology, on the one hand there is provided online Non-Destructive Testing and the method for screening Vigor of Zea mays L. Seeds；Separately
On the one hand, by the combination of two kinds of technologies, the acquisition of various dimensions is carried out to Vigor of Zea mays L. Seeds information, detection is greatly improved
Accuracy.Sample to having obtained optically and electrically information, single seed activity level is determined by Seed germination, is set up and is planted
Sub- optical/electrical/image information-motility model storehouse.Comparison model storehouse, you can know the activity level of current corn seed.
It is an object of the present invention to detect the high spectrum image and electrical impedance of corn seed by loseless method, with reference to maize seed
Sub-light spectrum/image information-motility model and the anti-motility model of resistance carry out the acquisition of various dimensions to Vigor of Zea mays L. Seeds information,
Online Non-Destructive Testing and screening to Vigor of Zea mays L. Seeds is realized by a kind of device.For this purpose, This invention provides one kind
The Vigor of Zea mays L. Seeds lossless audio coding of creative utilization high light spectrum image-forming technology and electrical impedance technology and the device of screening.
The content of the invention
The invention discloses a kind of Vigor of Zea mays L. Seeds detection means based on high light spectrum image-forming and electrical impedance, base is realized
Lossless audio coding, the corn for screening high vigor are carried out to the seed vitality of corn seed in high light spectrum image-forming and electrical impedance
Seed.The system solved from root prior seed vigor testing methods presence efficiency it is low, cost is high, accuracy is low, meeting
The drawback such as cause damage to seed.Innovative is combined high light spectrum image-forming technology and electrical impedance technology, utilizes corn seed
Optical characteristics and electrology characteristic, various dimensions acquisition corn seed vigor information.On the one hand it is online Non-Destructive Testing corn
Seed vitality provides apparatus and method, on the other hand also substantially increases the degree of accuracy.Meanwhile, the present apparatus can also be tied according to detection
Really, corn seed is screened in real time by mechanical device.The present apparatus has：Detect that lossless, automaticity is high, detect
Quickly, the advantages of screening efficient.
To realize above-mentioned goal of the invention, concrete technical scheme of the present invention is as follows：
A kind of Vigor of Zea mays L. Seeds detection means based on high light spectrum image-forming and electrical impedance, including：
The magazine of corn seed to be measured is stored, the bottom of the magazine is connected with one or more single seeded cunnings of output
Groove；
The conveyer belt of detection plate is installed, the detection plate is provided with the detection hole for the seed for receiving the chute output,
Electrode pair is provided with each detection hole；
The electric impedance analyzer being connected with the electrode pair, for applying exciting current to seed by electrode, according to electrode
Electrical impedance data between pair；
Hyperspectral imaging devices above conveyer belt, in detection hole corn seed carry out in real time into
Picture；
Computer, for carrying out comprehensive analysis to electrical impedance data and high-spectral data, is divided Vigor of Zea mays L. Seeds
Level；
Collection vessel positioned at conveyer belt one end, is inside provided with least two holding tanks, for receiving different motility grades
Corn seed.
In the present invention, corn seed makes batch corn seed to be measured be evenly distributed on conveyer belt by the chute of magazine bottom
On, the gap of its end and conveyer belt is smaller, therefore can ensure that each detection hole of detection plate on conveyer belt accommodates one just
Seed.
The corn seed of the detection hole, by setting experimental arrangement to apply a variety of different suitable excitations to corn seed
Electric current I, while impedance value and phase angle between detecting electrode, are automatically saved and record data, by the adjustment to experimental arrangement,
The exciting current of different electric currents, frequency, waveform can be provided, the need for meeting different scenes.
Described detection plate is single row configuration, is integrated with two or more detection holes for being mounted with electrode.Hole it is big
Small depth is corresponding with corn seed size, and a corn seed can be accommodated just.
It is with similar face product and two interlaced electrodes as preferred, described electrode pair.
As preferred, each electrode includes the arc being connected with electrode wires, and the arc of two electrodes is arranged symmetrically,
Two arcs are provided with the grizzly bar of relative extension and interleaved.
It is graphite material as preferred, described electrode pair.
In the present invention, electrode is two interlaced electrode structures with essentially identical surface area, is graphite material,
Be conducive to the extraction to corn seed impedance information.
Include wavelength band as preferred, described Hyperspectral imaging devices for not in 380-1023nm and 874-
1734nm two sets of imaging spectrometers.
In the present invention, hyperspectral imager includes：Two sets of imaging spectrometers (380-1023nm and 874-1734nm), CCD
Camera, XNP 1.4/17 (380-1023nm) and OLES15 (874-1734nm) imaging lens, Fiber-Lite DC950 lines
Light source and camera bellows.380-1023nm spectral resolution is 2.8nm, and 874-1734nm spectral resolution is 5nm.Camera lens is high
Degree, light source, conveyer belt movement velocity can adjust design, computer by the time for exposure, the intensity of light source, pixel gather frame
Number, the adjustment of resolution ratio, can carry out high light spectrum image-forming to the corn seed of different motion speed in different height, meet different fields
The need for scape.Wherein, high light spectrum image-forming data processing includes data prediction, feature extraction and model comparison.
Further, transmission belt surface is the non-reflective material of black, can different constant speed movements.
It is cuboid as preferred, described detection plate, several detection plates are spliced successively is fixed on the conveyer belt
On.
Multiple rows of detection plate is mounted with the present invention, on conveyer belt, using modularized design, can be dismantled, for difference
Various sizes of detection plate may be selected in big seedlet, with higher universality.
The built-in computer has editable experimental arrangement.Because varying environment disturbs larger to data, this module can
The pretreatment such as high-spectral data and electrical impedance data are normalized.It is many by principal component analysis, SVMs etc.
Algorithms of different is planted to analyze data.Corn seed spectrum-vigor and the anti-motility model of resistance are finally combined to seed vitality
Information is obtained, and is classified and is marked.
As preferred, the conveyer belt is provided with the guide rail in vertical transport direction, and being slidably fitted in for detection plate is described
Guide rail on.Described detection plate is controlled by computer, carries out transverse shifting according to the classification of the vigor of corn seed, controls corn
Seed is fallen into corresponding holding tank.
The detection plate can be moved in the vertical conveyors direction of motion, be carried out by the graded signal received corresponding
It is mobile, different motility grade corn seeds is dropped in different holding tanks in conveyer belt tail end collection vessel, realize efficient
Online screening.
Brief description of the drawings
Fig. 1 is the device of the Vigor of Zea mays L. Seeds on-line checking based on high light spectrum image-forming and electrical impedance；
Fig. 2 is hyperspectral imager side view；
Fig. 3 is the electrode structure of a detection unit；
Fig. 4 is that Vigor of Zea mays L. Seeds is detected and screening technique flow chart.
Embodiment
Describe the present invention in detail with reference to embodiment and accompanying drawing, but the present invention is not limited to this.
The device of Vigor of Zea mays L. Seeds based on electrical impedance detection as shown in Figure 1 includes：Computer 1, electric impedance analyzer
2, high light spectrum image-forming room 3, magazine 4, conveyer belt 5, collection vessel 6 and detection plate 7.Wherein, high light spectrum image-forming room 3 includes CCD phases
Machine 8, two sets of imaging spectrometer (380-1023nm and 874-1734nm) 9, XNP 1.4/17 (380-1023nm) and OLES15
The imaging lens 10 of (874-1734nm), Fiber-Lite DC950 line sources 11 and camera bellows.380-1023nm's is spectrally resolved
Rate is 2.8nm, and 874-1734nm spectral resolution is 5nm.
Corn seed to be measured is stored in magazine 5, the bottom of the magazine 5 is connected with exportable single seeded chute 12,
The size of chute 12 is adapted with the size of list simple grain, and inside is available for single seed to pass through just.
Detection plate 7 is slidably mounted on the guide rail in detection plate 5, and detection plate 7 is provided with the seed for receiving chute 12 to export
Detection hole 13, electrode pair is provided with each detection hole 13.The structure of electrode pair is as shown in Fig. 2 electrode pair is with identical table
Area and two interlaced electrodes, each electrode include the arc 16 being connected with electrode wires 15, the arc of two electrodes
Plate 16 is arranged symmetrically, and two arcs 16 are provided with the grizzly bar 17 of relative extension and interleaved.Electrode pair in the present embodiment
For graphite material.
As shown in figure 3, electric impedance analyzer 1 is connected by electrode wires 15 with the electrode pair in detection hole 13, by electrode to
Seed applies exciting current, detects seed vitality according to the impedance value between electrode pair and phase angle and is classified.
Collection vessel 7 is located at the tail end of conveyer belt one end, at least two holding tanks is inside provided with, for receiving different vigor etc.
The corn seed of level.
Detection plate 5 is provided with the guide rail 14 in vertical transport direction, the guide rail 14 being slidably fitted in of detection plate 7.The inspection
Drafting board 7 is controlled by computer 1, and transverse shifting is carried out according to the classification of the vigor of corn seed, and control corn seed, which falls into collection, to be held
In device 6 in corresponding holding tank.
Batch corn seed to be measured is put into magazine 4, seed enters conveyer belt by the chute of magazine 4.Due to chute
Size can only accommodate the corn seed of a normal size, and its end and the gap of conveyer belt 5 are smaller, therefore can ensure to pass
The each detection hole of detection plate 7 on band 5 is sent to accommodate a seed just.Computer 1 controls electric impedance analyzer 2 to maize seed immediately
Son applies a variety of different suitable excitation electric currents, while impedance value and phase angle between detecting electrode, computer 1 are automatically saved simultaneously
Record data.After computer 1 is analyzed electrical impedance data, preliminary classification and mark are carried out to corresponding corn seed.Corn seed
High light spectrum image-forming room 3 is entered with the constant speed set with conveyer belt 5, Fiber-Lite DC950 line sources 11 are provided suitably
Light source, two sets of imaging spectrometers 9 and CCD camera 8 carry out high light spectrum image-forming to corn seed.Because conveyer belt 5 is always with perseverance
Constant speed degree is moved, and imaging data is constantly collected, and is stored in computer 1.Computer 1 carries out pre- to imaging data in real time
The analyses such as processing, signature analysis, model comparison, secondary point is carried out in conjunction with electrical impedance data analysis result to Vigor of Zea mays L. Seeds
Level and mark.Detection plate 7 is moved according to the marking signal received in the vertical conveyors direction of motion.It can make to contain high work
The detection plate 7 of power seed is moved upward, and low vitality is moved down.Seed can drop containing many in the end of conveyer belt 5
In the collection vessel 6 of individual holding tank, the on-line checking to the corn seed of different seed vitalities and screening are realized.
As shown in figure 4, the detection method of the present embodiment comprises the following steps：
1) using batch corn seed to be measured as sample, seed is made to be evenly distributed on conveyer belt 5 using magazine, each detection
Hole 13 accommodates a seed；
2) electrode in detection hole 13 is connected with impedance instrument 2, and computer 1 controls impedance instrument 2 to maize seed
Son applies a variety of different suitable exciting currents, while impedance value and phase angle between detecting electrode.Computer 1 is recorded automatically
Data simultaneously carry out on-line analysis to the data collected, and preliminary assessment and classification are carried out to corresponding Vigor of Zea mays L. Seeds；
3) corn seed enters high light spectrum image-forming room 3 with conveyer belt 5 with constant speed, and hyperspectral imager is by testing journey
Ordered pair corn seed carries out real time imagery, and imaging data is automatically credited computer 1 and carries out online data analysis；
4) analysis result of the anti-data of the aggregate resistance of computer 1 and high light spectrum image-forming data, is carried out to Vigor of Zea mays L. Seeds
Second evaluation and classification, and the corn seed of different motility grades is marked, the detection plate 7 on control conveyer belt 5 is being hung down
Direct transfer and send tape motion direction accordingly to be moved；
5) seed falls into different holding tanks in the end of conveyer belt 5, and detection plate 7 resets；
6) 1) -5 are repeated) step, you can realize and on-line checking and screening are carried out to the seed vitality of corn seed.
The foregoing is only the preferable implementation example of the present invention, be not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.
Claims (9)
1. a kind of Vigor of Zea mays L. Seeds detection means based on high light spectrum image-forming and electrical impedance, it is characterised in that including：
The magazine of corn seed to be measured is stored, the bottom of the magazine is connected with one or more single seeded chutes of output；
The conveyer belt of detection plate is installed, the detection plate is provided with the detection hole for the seed for receiving the chute output, each
Electrode pair is provided with detection hole；
The electric impedance analyzer being connected with the electrode pair, for applying exciting current to seed by electrode, according between electrode pair
Electrical impedance data；
Hyperspectral imaging devices above conveyer belt, for carrying out real time imagery to the corn seed in detection hole；
Computer, for carrying out comprehensive analysis to electrical impedance data and high-spectral data, is classified to Vigor of Zea mays L. Seeds；
Collection vessel positioned at conveyer belt one end, is inside provided with least two holding tanks, the corn for receiving different motility grades
Seed.
2. the Vigor of Zea mays L. Seeds detection means as claimed in claim 1 based on high light spectrum image-forming and electrical impedance, its feature exists
In described electrode pair is with similar face product and two interlaced electrodes.
3. the Vigor of Zea mays L. Seeds detection means as claimed in claim 2 based on high light spectrum image-forming and electrical impedance, its feature exists
In each electrode includes the arc being connected with electrode wires, and the arc of two electrodes is arranged symmetrically, and two arcs are provided with
Relative extension and the grizzly bar of interleaved.
4. the Vigor of Zea mays L. Seeds detection means as claimed in claim 3 based on high light spectrum image-forming and electrical impedance, its feature exists
In described electrode pair is graphite material.
5. the Vigor of Zea mays L. Seeds detection means as claimed in claim 1 based on high light spectrum image-forming and electrical impedance, its feature exists
In described Hyperspectral imaging devices include wavelength band for not in 380-1023nm and 874-1734nm two sets of imaging spectrals
Instrument.
6. the Vigor of Zea mays L. Seeds detection means as claimed in claim 5 based on high light spectrum image-forming and electrical impedance, its feature exists
In the surface of the conveyer belt and detection plate is the non-reflective material of black.
7. the Vigor of Zea mays L. Seeds detection means as claimed in claim 1 based on high light spectrum image-forming and electrical impedance, its feature exists
In described detection plate is cuboid, and several detection plates are spliced on the conveyer belt successively.
8. the Vigor of Zea mays L. Seeds detection means as claimed in claim 7 based on high light spectrum image-forming and electrical impedance, its feature exists
In, the conveyer belt it is provided with the guide rail in vertical transport direction, detection plate is slidably fitted on described guide rail.
9. the Vigor of Zea mays L. Seeds detection means as claimed in claim 8 based on high light spectrum image-forming and electrical impedance, its feature exists
In described detection plate is controlled by computer, and transverse shifting is carried out according to the classification of the vigor of corn seed, and control corn seed falls
Enter in corresponding holding tank.
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| CN201710425116.3A CN107185850B (en) | 2017-06-06 | 2017-06-06 | Corn seed activity detection device based on hyperspectral imaging and electrical impedance |
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| CN201710425116.3A CN107185850B (en) | 2017-06-06 | 2017-06-06 | Corn seed activity detection device based on hyperspectral imaging and electrical impedance |
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| KR20190081469A (en) * | 2017-12-29 | 2019-07-09 | 순천대학교 산학협력단 | Machine vision based crop selection apparatus |
| CN110090809A (en) * | 2018-01-31 | 2019-08-06 | 西派特（北京）科技有限公司 | A kind of device of online high-speed lossless automatic sorting monoploid corn kernel |
| CN110169232A (en) * | 2019-05-29 | 2019-08-27 | 湖州师范学院 | A kind of automation single seed vigor separation system |
| CN110291869A (en) * | 2019-07-10 | 2019-10-01 | 中国农业大学 | Seed vitality non-destructive testing hierarchy system |
| CN110501065A (en) * | 2019-07-24 | 2019-11-26 | 南京农业大学 | Hybrid rice based on collision characteristic splits clever Seed inspection method |
| CN110586514A (en) * | 2019-10-24 | 2019-12-20 | 中国科学院长春光学精密机械与物理研究所 | Seed sorting equipment and method and seed activity detection device |
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| CN114026992A (en) * | 2021-10-12 | 2022-02-11 | 中国热带农业科学院热带作物品种资源研究所 | Seed vigor nondestructive test grading system |
| CN114088645A (en) * | 2021-10-15 | 2022-02-25 | 扬州大学 | Online rapid detection system and detection method for seed activity and health |
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