CN107164489A - A kind of primer pair and its application for Identification chinese herbs medicine material rhizoma alismatis - Google Patents
A kind of primer pair and its application for Identification chinese herbs medicine material rhizoma alismatis Download PDFInfo
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- CN107164489A CN107164489A CN201710421418.3A CN201710421418A CN107164489A CN 107164489 A CN107164489 A CN 107164489A CN 201710421418 A CN201710421418 A CN 201710421418A CN 107164489 A CN107164489 A CN 107164489A
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- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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Abstract
The invention discloses a kind of primer pair for Identification chinese herbs medicine material rhizoma alismatis and its application, the primer pair and its sequence are：P1, SEQ ID NO.1~2；P2, SEQ ID NO.3~4；P3, SEQ ID NO.5~6.(1) primer pair of the present invention causes the Germplasm Identification of Rhizoma Alismatis more to modernize, and improves product quality, quality discrimination and standardized production beneficial to rhizoma alismatis；(2) primer pair specificity of the present invention is good, sensitivity is high, stability is strong.
Description
Technical field
The invention belongs to biology field, it is related to a kind of method of use molecular biology method Identification chinese herbs medicine, has
Body is a kind of primer pair and its application for Identification chinese herbs medicine material rhizoma alismatis.
Background technology
Rhizoma alismatis alias marshy land, is Notes On Alism At Aceae, is used as medicine with stem tuber.Rhizoma alismatis distribution is very wide, and main product is in Sichuan, Fujian, river
West, the title for have " RHIZOMA ALISMATIS from Sichuan of China ", " Rhizoma Alismatis " and " river rhizoma alismatis ", are authentic medicinal herbs.By 2000 only, China's autonomic drug quantity
Up to 11,046 kinds, China has turned into medicinal plant in the world and maximum trading country, Chinese herbal medicine is calculated by tonnage
Exporting the economic development to China's economic development especially to current central/western China has highly important strategic importance.
However, in the world in the annual sales amount of Chinese herbal medicine market more than 16,000,000,000 dollars, China only occupies percentage therein
Five, and most of is bulk drug and health care medicinal.On the contrary, the crude drug of nearly 40 kinds of existing more than ten countries and regions
Thing is registered in China, and the annual import of China " midocean medicine " is more than 600,000,000 dollars, and its main cause has：Chinese herbal medicine effective ingredientses or
Principal component is difficult to by verification experimental verification；Heavy metal or residual toxicity content are higher, it is difficult to weighed with international standard, traditional Chinese medical theory
There is distance with modern analysis science, it is difficult to compared with doctor trained in Western medicine and insertion.Along with as each government and various circles of society are to doctor
The supervision in medicine market is more and more tighter, and interior ground medicinal industry will face the immense pressure from accession to WTO, and " midocean medicine " will increasingly
Threaten the Chinese traditional herbs intellectual property of China.As can be seen here, really to be solved in Chinese herbal medicine development the problem of is the mark of Chinese herbal medicine
Standardization, modernization and internationalization.
The original producton location of Ji Yangshi Fujian, Fujian authentic medicinal herbs rhizoma alismatis, for a long time, germplasm is not good, variety deterioration, makes its medicine
Effect and yield are influenceed by very big, and this causes my Fujian rhizoma alismatis at home or even in the world all in unfavorable status.Thus, it is
This authentic medicinal herbs of rhizoma alismatis is accelerated modernization, improve product quality, preferably meet WTO challenge and competitiveness, and make
Medicinal herb grower can therefrom obtain more material benefits, and resources advantage is converted into industrial advantage.
The content of the invention
The purpose of the present invention is：In order to overcome the defect of prior art so that the Germplasm Identification of Rhizoma Alismatis is more modern
Change, improve product quality, obtain a kind of method for being capable of specificity identification Chinese medicine rhizoma alismatis, quality discrimination and mark beneficial to rhizoma alismatis
Quasi- metaplasia production, the invention provides a kind of primer pair for Identification chinese herbs medicine material rhizoma alismatis and its application.
Technical scheme：A kind of primer pair for Identification chinese herbs medicine material rhizoma alismatis, the primer pair and its sequence are：P1, SEQ
ID NO.1~2；P2, SEQ ID NO.3~4；P3, SEQ ID NO.5~6.
It is preferred that, the 5 of described primer pair P1, P2 and P3 sense primer, end adds specific GC hairpin structures, described
The length of hairpin structure is 40bp, and sequence is SEQ ID NO.7.
The primer pair of table 1 and hairpin structure sequence
| Title | Sequence (5, -3) | SEQ ID NO. |
| P1-F | CCAGGCAATCACACCTGTGATGC | 1 |
| P1-R | CCTACTCCGCTTCTTGATATGC | 2 |
| P2-F | GTCCGTAGGAGAACCTACGG | 3 |
| P2-R | CTCCTCCGCTAATTGATATGTATTA | 4 |
| P3-F | CAGCGGATAACAATAACACACAGG | 5 |
| P3-R | AGGCAAGGCTTAACCCAGTCACGAC | 6 |
| Hairpin structure | CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGG | 7 |
Application of the primer pair in Identification chinese herbs medicine material rhizoma alismatis kit is prepared.
It is preferred that, the component of the kit includes：Primer pair, dNTP, archaeal dna polymerase, LC Green, reaction buffering
Liquid.
It is preferred that, include the step of the kit Identification chinese herbs medicine material rhizoma alismatis：
(1) genomic DNA of Samples of Alisma Orientalis is extracted；
(2) genomic DNA using step (1) extraction is template, using P1 as specific primer, carries out the amplification of first round PCR；
(3) take the amplified production of step (2), as the second wheel PCR template after 100~1000 times of dilution, using P2 as
Specific primer, carries out second and takes turns PCR amplifications；
(4) take the second wheel PCR expand product, dilution 10~100 times after as third round PCR template, using P3 as
Specific primer, carries out third round PCR amplifications；
(5) product of third round PCR amplifications is collected, and using agarose gel electrophoresis detection.
It is preferred that, the extracting method of the genomic DNA of the Samples of Alisma Orientalis is RNA isolation kit or modified CTAB method.
It is preferred that, the program of the PCR amplifications is：95 DEG C, 2min；95 DEG C, 30s, 52 DEG C, 30s, 72 DEG C, 55s, 35
Circulation；72 DEG C, 7min.
Beneficial effect：(1) primer pair of the present invention causes the Germplasm Identification of Rhizoma Alismatis more to modernize, and improves product
Quality, quality discrimination and standardized production beneficial to rhizoma alismatis；(2) primer pair specificity of the present invention is good, sensitivity is high, stably
Property is strong.
Brief description of the drawings
Fig. 1 is embodiment 1~3PCR qualification result electrophoretograms；
Wherein M is the Maker that molecular weight is 3000；1 is embodiment 1PCR product qualification results；2 be that embodiment 2PCR is produced
Thing qualification result；3 be embodiment 3PCR product qualification results.
Embodiment
Embodiment 1
A kind of primer pair for Identification chinese herbs medicine material rhizoma alismatis, the primer pair and its sequence are：P1, SEQ ID NO.1~
2；P2, SEQ ID NO.3~4；P3, SEQ ID NO.5~6.
The 5 of described primer pair P1, P2 and P3 sense primer, end adds specific GC hairpin structures, the hair fastener knot
The length of structure is 40bp, and sequence is SEQ ID NO.7.
Application of the primer pair in Identification chinese herbs medicine material rhizoma alismatis kit is prepared.
The component of the kit includes：Primer pair, dNTP, archaeal dna polymerase, LC Green, reaction buffer.
The step of kit Identification chinese herbs medicine material rhizoma alismatis, includes：
(1) genomic DNA of Samples of Alisma Orientalis is extracted；
(2) genomic DNA using step (1) extraction is template, using P1 as specific primer, carries out the amplification of first round PCR；
(3) amplified production of step (2) is taken, as the second wheel PCR template after 100 times of dilution, specificity is used as using P2
Primer, carries out second and takes turns PCR amplifications；
(4) product for taking the second wheel PCR to expand, as third round PCR template after 100 times of dilution, using P3 as special
Property primer, carry out third round PCR amplifications；
(5) product of third round PCR amplifications is collected, and using agarose gel electrophoresis detection.
The extracting method of the genomic DNA of the Samples of Alisma Orientalis is RNA isolation kit or modified CTAB method.
The program of PCR amplification is：95 DEG C, 2min；95 DEG C, 30s, 52 DEG C, 30s, 72 DEG C, 55s, 35 circulations；72
DEG C, 7min.
Embodiment 2
A kind of primer pair for Identification chinese herbs medicine material rhizoma alismatis, the primer pair and its sequence are：P1, SEQ ID NO.1~
2；P2, SEQ ID NO.3~4；P3, SEQ ID NO.5~6.
The 5 of described primer pair P1, P2 and P3 sense primer, end adds specific GC hairpin structures, the hair fastener knot
The length of structure is 40bp, and sequence is SEQ ID NO.7.
Application of the primer pair in Identification chinese herbs medicine material rhizoma alismatis kit is prepared.
The component of the kit includes：Primer pair, dNTP, archaeal dna polymerase, LC Green, reaction buffer.
The step of kit Identification chinese herbs medicine material rhizoma alismatis, includes：
(1) genomic DNA of Samples of Alisma Orientalis is extracted；
(2) genomic DNA using step (1) extraction is template, using P1 as specific primer, carries out the amplification of first round PCR；
(3) amplified production of step (2) is taken, as the second wheel PCR template after 100 times of dilution, specificity is used as using P2
Primer, carries out second and takes turns PCR amplifications；
(4) product for taking the second wheel PCR to expand, as third round PCR template after 10 times of dilution, specificity is used as using P3
Primer, carries out third round PCR amplifications；
(5) product of third round PCR amplifications is collected, and using agarose gel electrophoresis detection.
The extracting method of the genomic DNA of the Samples of Alisma Orientalis is RNA isolation kit or modified CTAB method.
The program of PCR amplification is：95 DEG C, 2min；95 DEG C, 30s, 52 DEG C, 30s, 72 DEG C, 55s, 35 circulations；72
DEG C, 7min.
Embodiment 3
A kind of primer pair for Identification chinese herbs medicine material rhizoma alismatis, the primer pair and its sequence are：P1, SEQ ID NO.1~
2；P2, SEQ ID NO.3~4；P3, SEQ ID NO.5~6.
The 5 of described primer pair P1, P2 and P3 sense primer, end adds specific GC hairpin structures, the hair fastener knot
The length of structure is 40bp, and sequence is SEQ ID NO.7.
Application of the primer pair in Identification chinese herbs medicine material rhizoma alismatis kit is prepared.
The component of the kit includes：Primer pair, dNTP, archaeal dna polymerase, LC Green, reaction buffer.
The step of kit Identification chinese herbs medicine material rhizoma alismatis, includes：
(1) genomic DNA of Samples of Alisma Orientalis is extracted；
(2) genomic DNA using step (1) extraction is template, using P1 as specific primer, carries out the amplification of first round PCR；
(3) amplified production of step (2) is taken, as the second wheel PCR template after 650 times of dilution, specificity is used as using P2
Primer, carries out second and takes turns PCR amplifications；
(4) product for taking the second wheel PCR to expand, as third round PCR template after 65 times of dilution, specificity is used as using P3
Primer, carries out third round PCR amplifications；
(5) product of third round PCR amplifications is collected, and using agarose gel electrophoresis detection.
The extracting method of the genomic DNA of the Samples of Alisma Orientalis is RNA isolation kit or modified CTAB method.
The program of PCR amplification is：95 DEG C, 2min；95 DEG C, 30s, 52 DEG C, 30s, 72 DEG C, 55s, 35 circulations；72
DEG C, 7min.
As shown in figure 1, the amplified production of embodiment 1~3 is carried out into electrophoresis detection, band is high-visible, and product is single.
SEQUENCE LISTING
<110>Suzhou City Li Liang Ji health industry Co., Ltd
<120>A kind of primer pair and its application for Identification chinese herbs medicine material rhizoma alismatis
<130>
<160> 7
<170> PatentIn version 3.3
<210> 1
<211> 23
<212> DNA
<213>Artificial sequence
<400> 1
ccaggcaatc acacctgtga tgc 23
<210> 2
<211> 22
<212> DNA
<213>Artificial sequence
<400> 2
cctactccgc ttcttgatat gc 22
<210> 3
<211> 20
<212> DNA
<213>Artificial sequence
<400> 3
gtccgtagga gaacctacgg 20
<210> 4
<211> 25
<212> DNA
<213>Artificial sequence
<400> 4
ctcctccgct aattgatatg tatta 25
<210> 5
<211> 24
<212> DNA
<213>Artificial sequence
<400> 5
cagcggataa caataacaca cagg 24
<210> 6
<211> 25
<212> DNA
<213>Artificial sequence
<400> 6
aggcaaggct taacccagtc acgac 25
<210> 7
<211> 40
<212> DNA
<213>Artificial sequence
<400> 7
cgcccgccgc gcgcggcggg cggggcgggg gcacgggggg 40
Claims (7)
1. a kind of primer pair for Identification chinese herbs medicine material rhizoma alismatis, it is characterised in that the primer pair and its sequence are：P1, SEQ
ID NO.1~2；P2, SEQ ID NO.3~4；P3, SEQ ID NO.5~6.
2. a kind of primer pair for Identification chinese herbs medicine material rhizoma alismatis according to claim 1, it is characterised in that the primer pair
The 5 of P1, P2 and P3 sense primer, end adds specific GC hairpin structures, and the length of the hairpin structure is 40bp, sequence
For SEQ ID NO.7.
3. application of the primer pair described in claim 1 or 2 in Identification chinese herbs medicine material rhizoma alismatis kit is prepared.
4. application according to claim 3, it is characterised in that the component of the kit includes：Primer pair, dNTP, DNA
Polymerase, LC Green, reaction buffer.
5. application according to claim 3, it is characterised in that include the step of the kit Identification chinese herbs medicine material rhizoma alismatis：
(1) genomic DNA of Samples of Alisma Orientalis is extracted；
(2) genomic DNA using step (1) extraction is template, using P1 as specific primer, carries out the amplification of first round PCR；
(3) amplified production of step (2) is taken, as the second wheel PCR template after 100~1000 times of dilution, using P2 as special
Property primer, carry out second take turns PCR amplification；
(4) product for taking the second wheel PCR to expand, as third round PCR template after 10~100 times of dilution, using P3 as special
Property primer, carry out third round PCR amplifications；
(5) product of third round PCR amplifications is collected, and using agarose gel electrophoresis detection.
6. application according to claim 5, it is characterised in that the extracting method of the genomic DNA of the Samples of Alisma Orientalis is
RNA isolation kit or modified CTAB method.
7. application according to claim 5, it is characterised in that the program of the PCR amplifications is：95 DEG C, 2min；95 DEG C,
30s, 52 DEG C, 30s, 72 DEG C, 55s, 35 circulations；72 DEG C, 7min.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107988415A (en) * | 2018-01-15 | 2018-05-04 | 福建省医学科学研究院 | A kind of primer pair and its application for differentiating Rhizoma Alismatis and RHIZOMA ALISMATIS from Sichuan of China |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013189047A1 (en) * | 2012-06-20 | 2013-12-27 | 南京大学 | Plant microrna identification and application thereof |
| CN103898204A (en) * | 2012-12-28 | 2014-07-02 | 财团法人工业技术研究院 | Method, database and primer pair for identifying herbal medicinal materials |
| CN104611328A (en) * | 2015-01-12 | 2015-05-13 | 中国医学科学院药用植物研究所 | Method for rapid identification of alisma orientale and alisma plantago-aquatica |
| CN105002148A (en) * | 2015-08-06 | 2015-10-28 | 南京中医药大学 | Alisma 3-hydroxy-3-methyl glutaric acyl coenzyme A reductase antibody preparation and detection method |
| CN106282390A (en) * | 2016-10-25 | 2017-01-04 | 中国医学科学院药用植物研究所 | Lycium barbarum and Fructus Lycii SNP rapid identification method |
-
2017
- 2017-06-07 CN CN201710421418.3A patent/CN107164489A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013189047A1 (en) * | 2012-06-20 | 2013-12-27 | 南京大学 | Plant microrna identification and application thereof |
| CN103898204A (en) * | 2012-12-28 | 2014-07-02 | 财团法人工业技术研究院 | Method, database and primer pair for identifying herbal medicinal materials |
| CN104611328A (en) * | 2015-01-12 | 2015-05-13 | 中国医学科学院药用植物研究所 | Method for rapid identification of alisma orientale and alisma plantago-aquatica |
| CN105002148A (en) * | 2015-08-06 | 2015-10-28 | 南京中医药大学 | Alisma 3-hydroxy-3-methyl glutaric acyl coenzyme A reductase antibody preparation and detection method |
| CN106282390A (en) * | 2016-10-25 | 2017-01-04 | 中国医学科学院药用植物研究所 | Lycium barbarum and Fructus Lycii SNP rapid identification method |
Non-Patent Citations (4)
| Title |
|---|
| XUEXIA LI ET AL: "Molecular Authentication of Alisma orientale by PCR-RFLP and ARMS", 《PLANTA MEDICA》 * |
| 孙军 等: "《医学生物化学与分子生物学实验》", 30 April 2008, 华中科技大学出版社 * |
| 耿超 等: "基于ITS2序列的建泽泻及其混伪品鉴定研究", 《南京中医药大学学报》 * |
| 黄琼林 等: "泽泻科植物DNA条形码的筛选研究", 《中华中医药杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107988415A (en) * | 2018-01-15 | 2018-05-04 | 福建省医学科学研究院 | A kind of primer pair and its application for differentiating Rhizoma Alismatis and RHIZOMA ALISMATIS from Sichuan of China |
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Application publication date: 20170915 |