CN107156864A - A kind of method that water-soluble fibre is prepared from root of kudzu vine waste material - Google Patents
A kind of method that water-soluble fibre is prepared from root of kudzu vine waste material Download PDFInfo
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- CN107156864A CN107156864A CN201710284295.3A CN201710284295A CN107156864A CN 107156864 A CN107156864 A CN 107156864A CN 201710284295 A CN201710284295 A CN 201710284295A CN 107156864 A CN107156864 A CN 107156864A
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- 235000010575 Pueraria lobata Nutrition 0.000 title claims abstract description 76
- 239000002699 waste material Substances 0.000 title claims abstract description 63
- 239000000835 fiber Substances 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 24
- 244000046146 Pueraria lobata Species 0.000 title claims description 69
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 66
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 15
- 241000219781 Pueraria montana var. lobata Species 0.000 claims abstract 7
- 239000000706 filtrate Substances 0.000 claims description 43
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 21
- 238000001556 precipitation Methods 0.000 claims description 17
- 239000006228 supernatant Substances 0.000 claims description 10
- 239000002244 precipitate Substances 0.000 claims description 9
- 238000005904 alkaline hydrolysis reaction Methods 0.000 claims description 8
- 239000012141 concentrate Substances 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims 1
- 229910052760 oxygen Inorganic materials 0.000 claims 1
- 239000001301 oxygen Substances 0.000 claims 1
- 241000219780 Pueraria Species 0.000 abstract description 4
- 230000007062 hydrolysis Effects 0.000 abstract description 4
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000012545 processing Methods 0.000 abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 3
- 238000004458 analytical method Methods 0.000 abstract description 2
- 238000007796 conventional method Methods 0.000 abstract description 2
- 235000013325 dietary fiber Nutrition 0.000 abstract description 2
- 238000000605 extraction Methods 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 3
- RXUWDKBZZLIASQ-UHFFFAOYSA-N Puerarin Natural products OCC1OC(Oc2c(O)cc(O)c3C(=O)C(=COc23)c4ccc(O)cc4)C(O)C(O)C1O RXUWDKBZZLIASQ-UHFFFAOYSA-N 0.000 description 3
- 206010037660 Pyrexia Diseases 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 238000012869 ethanol precipitation Methods 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- 235000021374 legumes Nutrition 0.000 description 3
- HKEAFJYKMMKDOR-VPRICQMDSA-N puerarin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=CC(C2=O)=C1OC=C2C1=CC=C(O)C=C1 HKEAFJYKMMKDOR-VPRICQMDSA-N 0.000 description 3
- 206010037844 rash Diseases 0.000 description 3
- 239000013049 sediment Substances 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 206010012735 Diarrhoea Diseases 0.000 description 2
- 208000010201 Exanthema Diseases 0.000 description 2
- 206010019233 Headaches Diseases 0.000 description 2
- 206010020772 Hypertension Diseases 0.000 description 2
- 240000006859 Jasminum officinale Species 0.000 description 2
- 235000010254 Jasminum officinale Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000036772 blood pressure Effects 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 201000005884 exanthem Diseases 0.000 description 2
- 231100000869 headache Toxicity 0.000 description 2
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 description 2
- 150000002515 isoflavone derivatives Chemical class 0.000 description 2
- 235000008696 isoflavones Nutrition 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- JKQOBWVOAYFWKG-UHFFFAOYSA-N molybdenum trioxide Chemical compound O=[Mo](=O)=O JKQOBWVOAYFWKG-UHFFFAOYSA-N 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- KKCBUQHMOMHUOY-UHFFFAOYSA-N sodium oxide Chemical compound [O-2].[Na+].[Na+] KKCBUQHMOMHUOY-UHFFFAOYSA-N 0.000 description 2
- 229910001948 sodium oxide Inorganic materials 0.000 description 2
- 206010013786 Dry skin Diseases 0.000 description 1
- 208000013016 Hypoglycemia Diseases 0.000 description 1
- 201000005505 Measles Diseases 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 241000731396 Pueraria montana var. thomsonii Species 0.000 description 1
- 210000001789 adipocyte Anatomy 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 150000001840 cholesterol esters Chemical class 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
- A23L33/22—Comminuted fibrous parts of plants, e.g. bagasse or pulp
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The present invention relates to a kind of method that water-soluble fibre is prepared from root of kudzu vine waste material, it is characterised in that comprises the steps of：1) crushing of Pachyrhizua angulatus waste material；2) sodium hydroxide hydrolysis is added；3) albumen in Pachyrhizua angulatus waste material is removed；4) alcohol analysis Pachyrhizua angulatus waste material water-soluble fibre；5) it is dried in vacuo；A kind of method that water-soluble fibre is prepared from root of kudzu vine waste material of the present invention, by extracting water-soluble fibre from root of kudzu vine waste material, change waste material improves the utilization rate of root of kudzu vine raw material into useful resources, adds the added value of root of kudzu vine processing.Basic hydrolysis extraction is employed, while also having obtained water insoluble Dietary Fiber from Pueraria, is compared compared with conventional method, while improving the gross production rate of root of kudzu vine fiber.This method technique is simple.
Description
Technical field
The present invention relates to a kind of method that water-soluble fibre is prepared from root of kudzu vine waste material.
Background technology
Elegant jessamine (Pueraria lobata (Willd.) Ohwi) is legume, belongs to medical and edible dual purpose plant, China's distribution
It is relatively wide, containing abundant pueraria starch in Radix Puerariae, containing a kind of material for being called isoflavones in Radix Puerariae, metabolism can be improved
Some indexs of syndrome, including blood pressure, high cholesterol ester and blood glucose.The special isoflavones of Puerarin, exists only in elegant jessamine
In, it may be possible to most beneficial material.Research shows that Puerarin helps to reduce blood pressure and cholesterolemia.But it have been found that
Maximum effect is regulation blood glucose.Diabetes and obesity can all cause blood Puerarin to be dropped by the method for reasonable layout glucose
Hypoglycemia, such as drops it off useful place (muscle), and reduces its distribution in fat cell and blood vessel.
Pachyrhizua angulatus (Pueraria lobata (Willd.) Ohwi var.thomsonii (Benth.) Vaniot der
Maesen) it is legume, belongs to medical and edible dual purpose plant, China is distributed more widely.At the beginning of curing mainly fever caused by exogenous pathogens, rigidity of nape and headache, measles
Rise, rash goes out that not smooth, warm disease is thirsty, quench one's thirst, diarrhea, dysentery, hypertension, coronary heart disease.Chinese medicine Pachyrhizua angulatus be legume pueraria lobata or
The dry root of sweet kudzu, generally excavates in autumn, season in winter two, removes after impurity, clean, run through, be cut into sheet and dry.Its property
It is cool, it is sweet, pungent, promoted the production of body fluid with expelling pathogenic factors from muscles and skin, promoting eruption, bring down a fever, Shengyang Zhixie the effects such as, cure mainly fever caused by exogenous pathogens headache, stiff nape and back, fiber crops
The diseases such as rash is impermeable, thirsty, diarrhea, hypertension.
Pachyrhizua angulatus waste material：After processing prepares Radix Pueraria thomsonii starch, the waste residue obtained after filtering, referred to as Pachyrhizua angulatus waste material.The root of kudzu vine
Starch plants are more.
Water-soluble fibre：Soluble fiber be one kind can dissolve or meet in water water slowly hydrolyse to water soluble molecules (or
Compound) fiber.
The content of the invention
It is an object of the invention to provide a kind of method that water-soluble fibre is prepared from root of kudzu vine waste material.
A kind of method that water-soluble fibre is prepared from root of kudzu vine waste material of the present invention, it is characterised in that comprise the steps of：A. powder
It is broken：Root of kudzu vine waste material is crushed；B. hydrolyze：5-8 times of sodium hydroxide solution of root of kudzu vine waste material quality is added, sodium hydroxide solution
Concentration is mass fraction 2-5%；C. remove the gred：The hydrolyzate of upper step is centrifuged with 3000-4000r/min, filter is removed
Slag；D. precipitate：The filtrate of upper step is adjusted into pH value with acetic acid, the pH value of filtrate is adjusted to 5.2-5.4, makes root of kudzu vine albumen
Precipitation, is centrifuged with 3000-4000r/min, is removed precipitation, is obtained filtrate；E. concentrate：By the filtrate hydroxide of upper step
Sodium adjusts pH value, and the pH value of filtrate is adjusted into 6.9-7.1, and the former root of kudzu vine waste material of concentration adds ethanol, precipitates 60-90min,
Then centrifuged with 3000-4000r/min；F. dry：Abandon supernatant, vacuum -0.08MPa, 75 DEG C of dry 90-
120min, obtains root of kudzu vine waste material water-soluble fibre.
A kind of method that water-soluble fibre is prepared from root of kudzu vine waste material of the present invention, it is characterised in that comprise the steps of：A. powder
It is broken：Root of kudzu vine waste material is crushed to 200-300 mesh；B. hydrolyze：Add 5-8 times of sodium hydroxide solution of root of kudzu vine waste material quality, hydrogen
The concentration of sodium hydroxide solution is mass fraction 2-5%, the alkaline hydrolysis 60-90min of temperature at 60-90 DEG C；C. remove the gred：Will be upper
The hydrolyzate of step is centrifuged with 3000-4000r/min, removes filter residue；D. precipitate：The filtrate of upper step is adjusted with acetic acid
Whole pH value, 5.2-5.4 is adjusted to by the pH value of filtrate, is made root of kudzu vine albumen precipitation, is centrifuged with 3000-4000r/min, is gone
Except precipitation, filtrate is obtained；E. concentrate：The filtrate of upper step is adjusted into pH value with sodium hydroxide, the pH value of filtrate is adjusted to 6.9-
7.1,2-3 times of former root of kudzu vine waste material quality is concentrated into, 5-7 times of 95% ethanol of former kudzu-vine root powder quality is added, precipitates 60-
90min, is then centrifuged with 3000-4000r/min；F. dry：Abandon supernatant, vacuum -0.08MPa, 75 DEG C of dryings
90-120min, obtains root of kudzu vine waste material water-soluble fibre.
A kind of method that water-soluble fibre is prepared from root of kudzu vine waste material of the present invention, is related to water-soluble fibre in a kind of Pachyrhizua angulatus waste material
Extracting method, comprise the following steps：
1) crushing of Pachyrhizua angulatus waste material；
2) sodium hydroxide hydrolysis is added；
3) albumen in Pachyrhizua angulatus waste material is removed；
4) alcohol analysis Pachyrhizua angulatus waste material water-soluble fibre；
5) it is dried in vacuo；
A kind of beneficial effect for the method that water-soluble fibre is prepared from root of kudzu vine waste material of the present invention：
(1) by extracting water-soluble fibre from root of kudzu vine waste material, change waste material improves the profit of root of kudzu vine raw material into useful resources
With rate, the added value of root of kudzu vine processing is added.
(2) basic hydrolysis extraction is employed, while water insoluble Dietary Fiber from Pueraria has also been obtained, compared with conventional method phase
Than while improving the gross production rate of root of kudzu vine fiber.
(3) this method technique is simple.
Embodiment
With reference to embodiment, the present invention is described in further detail, but the embodiment not limited to this of invention.
A kind of method for preparing water-soluble fibre from root of kudzu vine waste material of the present invention of embodiment 1 is comprised the steps of：A. crush：
Root of kudzu vine waste material is crushed to 200 mesh；B. hydrolyze：5 times of sodium hydroxide solutions of root of kudzu vine waste material quality are added, sodium hydroxide solution
Concentration is mass fraction 2%, the alkaline hydrolysis 60min of temperature at 60 DEG C；C. remove the gred：By the hydrolyzate of upper step with 3000r/min
Centrifuge, remove filter residue；D. precipitate：The filtrate of upper step is adjusted into pH value with acetic acid, the pH value of filtrate is adjusted to 5.2,
Make root of kudzu vine albumen precipitation, centrifuged with 3000r/min, remove precipitation, obtain filtrate；E. concentrate：By the filtrate hydrogen of upper step
Sodium oxide molybdena adjusts pH value, and the pH value of filtrate is adjusted into 6.9, is concentrated into 2 times of former root of kudzu vine waste material quality, adds former root of kudzu vine silty
5 times of 95% ethanol of amount, is precipitated 60min, is then centrifuged with 3000r/min；F. dry：Abandon supernatant, vacuum-
0.08MPa, 75 DEG C of dry 90min, obtains root of kudzu vine waste material water-soluble fibre.
A kind of method for preparing water-soluble fibre from root of kudzu vine waste material of the present invention of embodiment 2 is comprised the steps of：A. crush：
Root of kudzu vine waste material is crushed to 300 mesh；B. hydrolyze：8 times of sodium hydroxide solutions of root of kudzu vine waste material quality are added, sodium hydroxide solution
Concentration is mass fraction 5%, the alkaline hydrolysis 90min of temperature at 90 DEG C；C. remove the gred：By the hydrolyzate of upper step with 4000r/min
Centrifuge, remove filter residue；D. precipitate：The filtrate of upper step is adjusted into pH value with acetic acid, the pH value of filtrate is adjusted to 5.4,
Make root of kudzu vine albumen precipitation, centrifuged with 4000r/min, remove precipitation, obtain filtrate；E. concentrate：By the filtrate hydrogen of upper step
Sodium oxide molybdena adjusts pH value, and the pH value of filtrate is adjusted into 7.1, is concentrated into 3 times of former root of kudzu vine waste material quality, adds former root of kudzu vine silty
7 times of 95% ethanol of amount, is precipitated 90min, is then centrifuged with 4000r/min；F. dry：Abandon supernatant, vacuum-
0.08MPa, 75 DEG C of dry 120min, obtains root of kudzu vine waste material water-soluble fibre.
A kind of method for preparing water-soluble fibre from root of kudzu vine waste material of the present invention of embodiment 3 is comprised the steps of：A. crush：
Root of kudzu vine waste material is crushed to 250 mesh；B. hydrolyze：7 times of sodium hydroxide solutions of root of kudzu vine waste material quality are added, sodium hydroxide solution
Concentration is mass fraction 4%, the alkaline hydrolysis 80min of temperature at 60-90 DEG C；C. remove the gred：By the hydrolyzate of upper step with 3500r/
Min is centrifuged, and removes filter residue；D. precipitate：The filtrate of upper step is adjusted into pH value with acetic acid, the pH value of filtrate is adjusted to
5.3, make root of kudzu vine albumen precipitation, centrifuged with 3500r/min, remove precipitation, obtain filtrate；E. concentrate：By the filtrate of upper step
PH value is adjusted with sodium hydroxide, the pH value of filtrate is adjusted to 7.0,2.5 times of former root of kudzu vine waste material quality are concentrated into, former Pueraria lobota is added
6 times of 95% ethanol of root silty amount, is precipitated 80min, is then centrifuged with 3500r/min；F. dry：Supernatant is abandoned, very
Reciprocal of duty cycle -0.08MPa, 75 DEG C of dry 110min, obtain root of kudzu vine waste material water-soluble fibre.
A kind of method for preparing water-soluble fibre from root of kudzu vine waste material of the present invention of embodiment 4 is comprised the steps of：
(1) 1 kilogram of RADIX PUERARIAE waste material is weighed, 200-300 mesh is crushed to；
(2) 5 kilograms of 5% sodium hydroxide, the alkaline hydrolysis 90min of temperature at 90 DEG C are added；
(3) above-mentioned (2) are centrifuged with 3500r/min；
(4) pH value 5.3 of above-mentioned (3) filtrate is adjusted, makes root of kudzu vine albumen precipitation, 3500r/min is centrifuged, it is heavy to remove
Form sediment, obtain filtrate；
(5) pH value 7.0 of above-mentioned (4) filtrate is adjusted, 3 kilograms are concentrated into, 6 kilograms of 95% ethanol precipitation 60- is added
90min, is then centrifuged with 3500r/min；
(6) dry, abandon supernatant, vacuum -0.08MPa, 75 DEG C of drys 110min are cooled to room temperature, claim the root of kudzu vine is useless
Expect 31 grams of water-soluble fibre.
A kind of method for preparing water-soluble fibre from root of kudzu vine waste material of the present invention of embodiment 5 is comprised the steps of：(1) weigh
5 kilograms of RADIX PUERARIAE waste material, is crushed to 200-300 mesh；
(2) 26 kilograms of 5% sodium hydroxide, the alkaline hydrolysis 90min of temperature at 90 DEG C are added；
(3) above-mentioned (2) are centrifuged with 3500r/min；
(4) pH value 5.2 of above-mentioned (3) filtrate is adjusted, makes root of kudzu vine albumen precipitation, 3500r/min is centrifuged, it is heavy to remove
Form sediment, obtain filtrate；
(5) pH value 7.0 of above-mentioned (4) filtrate is adjusted, 14.5 kilograms are concentrated into, 29 kilograms of 95% ethanol precipitation is added
60-90min, is then centrifuged with 3500r/min；
(6) dry, abandon supernatant, vacuum -0.08MPa, 75 DEG C of drys 120min are cooled to room temperature, claim the root of kudzu vine is useless
Expect 167 grams of water-soluble fibre.
A kind of method for preparing water-soluble fibre from root of kudzu vine waste material of the present invention of embodiment 6 is comprised the steps of：
(1) 10 kilograms of RADIX PUERARIAE waste material is weighed, 200-300 mesh is crushed to；
(2) 50 kilograms of 5% sodium hydroxide, the alkaline hydrolysis 90min of temperature at 90 DEG C are added；
(3) above-mentioned (2) are centrifuged with 3500r/min；
(4) pH value 5.2 of above-mentioned (3) filtrate is adjusted, makes root of kudzu vine albumen precipitation, 3500r/min is centrifuged, it is heavy to remove
Form sediment, obtain filtrate；
(5) pH value 7.0 of above-mentioned (4) filtrate is adjusted, 31 kilograms are concentrated into, 59 kilograms of 95% ethanol precipitation 60- is added
90min, is then centrifuged with 3500r/min；
(6) dry, abandon supernatant, vacuum -0.08MPa, 75 DEG C of drys 120min are cooled to room temperature, claim the root of kudzu vine is useless
Expect 347 grams of water-soluble fibre.
It is described above, it is merely illustrative of the technical solution of the present invention and unrestricted, those of ordinary skill in the art are to this hair
Other modifications or equivalent substitution that bright technical scheme is made, without departing from the spirit and scope of technical solution of the present invention,
It all should cover among scope of the presently claimed invention.
Claims (2)
1. a kind of method that water-soluble fibre is prepared from root of kudzu vine waste material, it is characterised in that comprise the steps of：A. crush：By the root of kudzu vine
Waste material is crushed；B. hydrolyze：5-8 times of sodium hydroxide solution of root of kudzu vine waste material quality is added, the concentration of sodium hydroxide solution is quality
Fraction 2-5%；C. remove the gred：The hydrolyzate of upper step is centrifuged with 3000-4000r/min, filter residue is removed；D. precipitate：
The filtrate of upper step is adjusted into pH value with acetic acid, the pH value of filtrate is adjusted to 5.2-5.4, makes root of kudzu vine albumen precipitation, is used
3000-4000r/min is centrifuged, and is removed precipitation, is obtained filtrate；E. concentrate：The filtrate of upper step is adjusted into pH with sodium hydroxide
Value, 6.9-7.1 is adjusted to by the pH value of filtrate, the former root of kudzu vine waste material of concentration, is added ethanol, is precipitated 60-90min, then with
3000-4000r/min is centrifuged；F. dry：Supernatant is abandoned, vacuum -0.08MPa, 75 DEG C of dry 90-120min are obtained
To root of kudzu vine waste material water-soluble fibre.
2. a kind of method that water-soluble fibre is prepared from root of kudzu vine waste material according to claim 1, it is characterised in that comprising with
Lower step：A. crush：Root of kudzu vine waste material is crushed to 200-300 mesh；B. hydrolyze：Add 5-8 times of hydrogen-oxygen of root of kudzu vine waste material quality
Change sodium solution, the concentration of sodium hydroxide solution is mass fraction 2-5%, the alkaline hydrolysis 60-90min of temperature at 60-90 DEG C；
C. remove the gred：The hydrolyzate of upper step is centrifuged with 3000-4000r/min, filter residue is removed；D. precipitate：By the filter of upper step
Liquid adjusts pH value with acetic acid, and the pH value of filtrate is adjusted into 5.2-5.4, makes root of kudzu vine albumen precipitation, uses 3000-4000r/min
Centrifuge, remove precipitation, obtain filtrate；E. concentrate：The filtrate of upper step is adjusted into pH value with sodium hydroxide, by the pH value of filtrate
6.9-7.1 is adjusted to, 2-3 times of former root of kudzu vine waste material quality is concentrated into, 5-7 times of 95% second of former kudzu-vine root powder quality is added
Alcohol, is precipitated 60-90min, is then centrifuged with 3000-4000r/min；F. dry：Abandon supernatant, vacuum-
0.08MPa, 75 DEG C of dry 90-120min, obtains root of kudzu vine waste material water-soluble fibre.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710284295.3A CN107156864B (en) | 2017-04-26 | 2017-04-26 | Method for preparing water-soluble fiber from pueraria lobata waste |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710284295.3A CN107156864B (en) | 2017-04-26 | 2017-04-26 | Method for preparing water-soluble fiber from pueraria lobata waste |
Publications (2)
| Publication Number | Publication Date |
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| CN107156864A true CN107156864A (en) | 2017-09-15 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109588730A (en) * | 2017-09-30 | 2019-04-09 | 浙江尖峰药业有限公司 | The method and dietary fiber purposes of dietary fiber are prepared using pueraria root residue |
| CN109602027A (en) * | 2018-12-10 | 2019-04-12 | 武汉轻工大学 | A kind of method that extrusion assistance enzymolysis extracts soluble dietary fiber in pueraria root residue |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050136780A1 (en) * | 2003-12-17 | 2005-06-23 | Kimberly-Clark Worldwide, Inc. | Water dispersible, pre-saturated wiping products |
| CN1765923A (en) * | 2004-10-29 | 2006-05-03 | 浙江工业大学 | Method for extracting and separating protein from the root of kudzu vine |
| CN102450536A (en) * | 2010-10-28 | 2012-05-16 | 纽威(武汉)生物科技有限公司 | Composition for suppressing appetite, removing toxin, losing weight and eliminating fat |
| CN103623776A (en) * | 2013-11-22 | 2014-03-12 | 重庆工商大学 | Method for preparing iron-based magnetic activated carbon by catalytic activation of radix puerariae waste residue with compound enzyme |
| CN104940280A (en) * | 2014-03-24 | 2015-09-30 | 天津道谷生物科技有限公司 | Method for extracting total flavones from radix puerariae employing enzyme preparation |
-
2017
- 2017-04-26 CN CN201710284295.3A patent/CN107156864B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050136780A1 (en) * | 2003-12-17 | 2005-06-23 | Kimberly-Clark Worldwide, Inc. | Water dispersible, pre-saturated wiping products |
| CN1765923A (en) * | 2004-10-29 | 2006-05-03 | 浙江工业大学 | Method for extracting and separating protein from the root of kudzu vine |
| CN102450536A (en) * | 2010-10-28 | 2012-05-16 | 纽威(武汉)生物科技有限公司 | Composition for suppressing appetite, removing toxin, losing weight and eliminating fat |
| CN103623776A (en) * | 2013-11-22 | 2014-03-12 | 重庆工商大学 | Method for preparing iron-based magnetic activated carbon by catalytic activation of radix puerariae waste residue with compound enzyme |
| CN104940280A (en) * | 2014-03-24 | 2015-09-30 | 天津道谷生物科技有限公司 | Method for extracting total flavones from radix puerariae employing enzyme preparation |
Non-Patent Citations (2)
| Title |
|---|
| 万凯: ""班处理十吨鲜葛根的加工厂设计"", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
| 梅新等，: ""葛渣膳食纤维的过氧化氢改性工艺研究"", 《湖北农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109588730A (en) * | 2017-09-30 | 2019-04-09 | 浙江尖峰药业有限公司 | The method and dietary fiber purposes of dietary fiber are prepared using pueraria root residue |
| CN109602027A (en) * | 2018-12-10 | 2019-04-12 | 武汉轻工大学 | A kind of method that extrusion assistance enzymolysis extracts soluble dietary fiber in pueraria root residue |
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