CN107156842A - Anti-breast cancer health products containing rice husk flavones - Google Patents
Anti-breast cancer health products containing rice husk flavones Download PDFInfo
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- CN107156842A CN107156842A CN201710362937.7A CN201710362937A CN107156842A CN 107156842 A CN107156842 A CN 107156842A CN 201710362937 A CN201710362937 A CN 201710362937A CN 107156842 A CN107156842 A CN 107156842A
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- flavones
- rice husk
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- breast cancer
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- 235000009566 rice Nutrition 0.000 title claims abstract description 65
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- 235000011949 flavones Nutrition 0.000 title claims abstract description 60
- 150000002213 flavones Chemical class 0.000 title claims abstract description 46
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- 239000007788 liquid Substances 0.000 claims description 16
- 238000000108 ultra-filtration Methods 0.000 claims description 15
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- 102000004142 Trypsin Human genes 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/236—Ligusticum (licorice-root)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/487—Psoralea
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/64—Orobanchaceae (Broom-rape family)
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The present invention discloses a kind of anti-breast cancer health products containing rice husk flavones, is made up of following raw materials by weight：Rice husk flavones 50 70%, cistanche extracts 5 10%, Fructus Psoraleae extract 5 10%, Viscum album extract 5 10%, Rhizoma Chuanxiong extract 5 10%, Placenta Hominis extract 5 10%.The present invention passes through the experimental study to people's breast cancer cells ira vitro, it was found that the composition of rice husk flavones has good inhibiting effect to tumor cell in vitro, there is the effect of highly significant for the preventing and treating of breast cancer, relative to prior art, the flavones and Chinese medicine composition for the treatment of breast cancer obtained by the present invention, cost are low, and technique is simple, treatment breast cancer curative effect can not only further be improved, moreover it is possible to significantly reduce the side effect of chemical drug treatment.
Description
Technical field
The invention belongs to field of health care products, and in particular to a kind of health care containing rice husk flavones with anti-breast cancer function
Product.
Background technology
Tumour is a class common disease of serious threat human health, is one of three big killers of human health, wherein breast
Gland cancer is a kind of common malignant tumour for having a strong impact on the physically and mentally healthy even threat to life of women, according to statistics, breast cancer
The incidence of disease accounts for the 7-10% of the various malignant tumours of whole body, and uterine cancer is only second in women, finds effective cancer therapy drug and side
Method, is the important topic that world medicine faces.Chinese medicine is the powerful mean that motherland's medical use carrys out disease preventing and treating, with chemical synthetic drug
Thing is compared, in addition to Chinese medicine is except having the advantages that convenient material drawing, cheap and toxic side effect is small, it is often more important that, face in mitigation
Bed symptom, improves life quality, prevents relapse and metastasis, extends life cycle, pre- preventing tumor, improves patient's immunity, and enhancing is put, changed
Sensitiveness is treated, mitigates many aspects such as Radiotherapy chemotherapy adverse reaction and plays an important role.Meanwhile, with the development of the society, production
Notice is increasingly turned to crude drug by the development of power, modern from chemical combination patent medicine, it is desirable to developed more from natural drug
Safely and effectively new drug is to substitute the former, so as to reduce the generation of medicine source disease.Therefore, development Chinese herbal medicine has not only passed on China
National 1,100 elite, it may have large market prospect and economic benefit.
The content of the invention
Set out, make the present invention seeks to the processing byproduct rice husk for the grass family cereal crops paddy rice planted extensively with China
There is provided a kind of natural health-care products of anti-breast cancer for standby rice husk flavones and the mixing of other Chinese medical extracts.
The technical solution adopted by the present invention is：
Anti-breast cancer health products containing rice husk flavones, are made up of following raw materials by weight：
Rice husk flavones 50-70%, cistanche extracts 5-10%, Fructus Psoraleae extract 5-10%,
Viscum album extract 5-10%, Rhizoma Chuanxiong extract 5-10%, Placenta Hominis extract 5-10%.
The described anti-breast cancer health products containing rice husk flavones, the preparation method of the rice husk flavones is as follows：
1) cellulase degradation is extracted：Solid-liquid ratio in mass ratio 1:20-1:30, rice husk powder is dissolved in water, cellulase
Addition is 150-180U/g, carries out hot water extraction, and it is 40-60 DEG C to control temperature, pH value range 4.0-6.0, and enzymolysis time is
1-3h, is centrifuged, and filtering obtains that cellulase degradation extract solution is standby, sediment A proceeds ethanol assisted extraction；
2) ethanol assisted extraction：Solid-liquid ratio in mass ratio 1:10-1:30, sediment A is dissolved in ethanol, volume fraction of ethanol
For 50-70%, extraction time is 2-3h, is centrifuged, and filtering obtains that ethanol assisted extraction liquid is standby, sediment B proceeds ultrasonic leaching
Carry；
3) ultrasound extraction：Solid-liquid ratio presses in mass ratio 1:20-1:30, sediment B is dissolved in ethanol, volume fraction of ethanol is
50-70%, ultrasonic power is 160-200W, and ultrasonic time is 20-40min, and suction filtration obtains ultrasonic extraction extract solution standby；
4) merge cellulase degradation extract solution, ethanol assisted extraction liquid and ultrasound extraction extract solution, total extract solution is revolved
Turn to be concentrated by evaporation, after filter paper prefiltration, ultrafiltration is carried out with filter membrane, ultrafiltrate sterile cryo is dried, and obtains thick rice husk flavones dry powder.
The described anti-breast cancer health products containing rice husk flavones, the preparation method of the cistanche extracts：In room temperature
Under the conditions of, weigh the saline cistanche solid powder for being ground into 200 mesh；Difference in mass ratio 1:10 ratios weigh saline cistanche solid powder
And deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, the drying of ultrafiltrate sterile cryo,
Obtain cistanche extracts.
The described anti-breast cancer health products containing rice husk flavones, the preparation method of the Fructus Psoraleae extract：In room temperature
Under the conditions of, weigh the psoralea corylifolia solid powder for being ground into 200 mesh；Difference in mass ratio 1:10 ratios weigh psoralea corylifolia solid powder
And deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, the drying of ultrafiltrate sterile cryo,
Obtain Fructus Psoraleae extract.
The described anti-breast cancer health products containing rice husk flavones, the preparation method of the Viscum album extract：In room temperature
Under the conditions of, weigh the Ramulus Taxilli solid powder for being ground into 200 mesh；Difference in mass ratio 1:10 ratios weigh Ramulus Taxilli solid powder
And deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, the drying of ultrafiltrate sterile cryo,
Obtain Viscum album extract.
The described anti-breast cancer health products containing rice husk flavones, the preparation method of the Rhizoma Chuanxiong extract：In room temperature bar
Under part, the Ligusticum wallichii solid powder for being ground into 200 mesh is weighed；Difference in mass ratio 1:10 ratios weigh Ligusticum wallichii solid powder and go from
Sub- water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate sterile cryo is dried, and obtains Ligusticum wallichii
Extract.
The described anti-breast cancer health products containing rice husk flavones, the preparation method of the Placenta Hominis extract：In room temperature
Under the conditions of, weigh the dried human placenta solid powder for being ground into 200 mesh；Difference in mass ratio 1:10 ratios weigh dried human placenta solid powder
And deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, the drying of ultrafiltrate sterile cryo,
Obtain Placenta Hominis extract.
The beneficial effects of the invention are as follows：Using the flavones of paddy rice processing byproduct rice husk powder extraction as active material,
Anti-breast cancer health products or medicine are prepared, extracting method is simple, in the absence of the toxic side effect of synthesis medicament, the requirement to equipment
Low, intermediate product and end-product are nontoxic, safe, and production cost is low, and take and carry very convenient, absorb fast
Speed and effect is good, therefore possess very broad mass market prospect, it is adapted to industrial production.
Initiative technology of the invention is by the core functionality raw material of rice husk flavones anti-breast cancer the most, according to modern times battalion
Foster model is reasonably combined, science combination application, forms flavones, Chinese herbal medicine mixed health product with anti-breast cancer.The present invention
By the experimental study to people's breast cancer cells ira vitro, find the composition of rice husk flavones has preferably to tumor cell in vitro
Inhibitory action, has the effect of highly significant for the preventing and treating of breast cancer, relative to prior art, the treatment breast obtained by the present invention
The flavones and Chinese medicine composition of gland cancer, cost are low, and technique is simple, can not only further improve treatment breast cancer curative effect, moreover it is possible to aobvious
Write the side effect of reduction chemical drug treatment.
Brief description of the drawings
Fig. 1 is that rice husk flavone composition influences on MCF-7 cell strainHJ2mm cell growth.
Fig. 2 is the colony test result that rice husk flavone composition suppresses the formation of MCF-7 cell colonies.
Fig. 3 is that rice husk flavone composition suppresses MCF-7 cell migration assay results.
Embodiment
Anti-breast cancer health products of the embodiment 1 containing rice husk flavones
(1) preparation of rice husk flavones
Rice husk flavones, specific step are extracted using the process combination of cellulase degradation-alcohol steep-ultrasonic wave method of fractional steps
It is rapid as follows：
1) cellulase degradation：Rice husk is gone into the removal of impurity, dried, size-reduced machine is crushed, 60 mesh sieves is crossed, powdered rice hulls is made
End.Solid-liquid ratio in mass ratio 1:30, rice husk powder is dissolved in water, cellulase addition is 150U/g, carries out hot water extraction, control
Temperature processed is 50 DEG C, pH=5.0, and enzymolysis time is 2h, and 4000r/min carries out centrifugation 15min, and filtering obtains cellulase degradation
Extract solution is standby, and sediment A proceeds ethanol Assisted Extraction；
2) ethanol assisted extraction：Solid-liquid ratio presses in mass ratio 1：20, sediment A is dissolved in ethanol, volume fraction of ethanol is
60%, extraction time is 3h, is centrifuged, and filtering obtains that ethanol assisted extraction liquid is standby, sediment B proceeds ultrasonic extraction；
3) ultrasound extraction：Solid-liquid ratio presses in mass ratio 1:20, sediment is dissolved in ethanol, volume fraction of ethanol is 60%,
Ultrasonic power is 180W, and ultrasonic time is 30min, and suction filtration obtains ultrasonic extraction extract solution standby；
4) merge cellulase degradation extract solution, ethanol assisted extraction liquid and ultrasound extraction extract solution, total extract solution is revolved
Turn to be concentrated by evaporation, after filter paper prefiltration, ultrafiltration is carried out with filter membrane, ultrafiltrate sterile cryo is dried, and obtains thick rice husk flavones dry powder.
(2) preparation of cistanche extracts
At ambient temperature, the saline cistanche solid powder for being ground into 200 mesh is weighed；In mass ratio 1:10 ratios weigh meat desert
Rong's solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate without
Bacterium is freeze-dried, and obtains cistanche extracts.
(3) preparation of Fructus Psoraleae extract
At ambient temperature, the psoralea corylifolia solid powder for being ground into 200 mesh is weighed；In mass ratio 1:10 ratios weigh Psoralen
Fat solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate without
Bacterium is freeze-dried, and obtains Fructus Psoraleae extract.
(4) preparation of Viscum album extract
At ambient temperature, the Ramulus Taxilli solid powder for being ground into 200 mesh is weighed；In mass ratio 1:10 ratios weigh mulberry and posted
Raw solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate without
Bacterium is freeze-dried, and obtains Viscum album extract.
(5) preparation of Rhizoma Chuanxiong extract
At ambient temperature, the Ligusticum wallichii solid powder for being ground into 200 mesh is weighed；In mass ratio 1:10 ratios weigh Ligusticum wallichii and consolidated
Body powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate are sterile cold
It is lyophilized dry, obtain Rhizoma Chuanxiong extract.
(6) preparation of Placenta Hominis extract
At ambient temperature, the dried human placenta solid powder for being ground into 200 mesh is weighed；In mass ratio 1:10 ratios weigh purple river
Car solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate without
Bacterium is freeze-dried, and obtains Placenta Hominis extract.
(7) anti-breast cancer Halth-care composition (abbreviation rice husk flavone composition) containing rice husk flavones
It is made up of following raw materials by weight：
Rice husk flavones 70%, cistanche extracts 10%, Fructus Psoraleae extract 5%,
Viscum album extract 5%, Rhizoma Chuanxiong extract 5%, Placenta Hominis extract 5%.
(8) MTT experiment
The present embodiment uses MCF-7 cell strainHJ2mm cell, is resisted in vitro using mtt assay detection rice husk flavone composition
Cancer activity, experimental procedure is as follows：
1) breast cancer cancerous cell line (MCF-7) cell in growth period of taking the logarithm is inoculated in 96 orifice plates, and incubated overnight pastes it
Wall；
2) concentration (12.5,25,50mg/ml) of rice husk flavone composition；
3) MTT (5mg/ml) is added after 24h；
4) and then 96 orifice plates are placed in 37 DEG C of constant incubators and continue to cultivate 4h；
5) after processing terminates, MTT is suctioned out, 150 μ l DMSO is added, is completely dissolved first a ceremonial jade-ladle, used in libation crystal；
6) increment of MCF-7 cells is assessed by measuring absorbance of each hole at 490nm；As a result such as Fig. 1 institutes
Show.
As shown in figure 1, the rice husk flavone composition (12.5,25 and 50mg/ml) of various concentrations is incubated MCF-7 cells respectively
24h, then utilizes MTT colorimetric determinations.As a result show, after handling breast cancer cell for a period of time, concentration is 25,50mg/ml
Rice husk flavone composition show significant inhibitory action, concentration is that 50mg/ml group inhibiting rates reach after 24h
78%, as a result show, rice husk flavone composition can suppress human breast cancer cell growth in vitro.
(9) Colony forming is tested
The breast cancer cell of different treatment groups is inoculated on orifice plate with the density of every 1000 cells in hole, and overnight incubation makes
Its is adherent.25 and 50mg/ml rice husk flavone composition culture 10d is separately added into, culture medium is suctioned out, is carefully washed with PBS per hole
Twice.Colony is fixed into 20min using methanol, then with 0.5% violet staining 15min, is rinsed and taken pictures with clear water, as a result
As shown in Figure 2.
As shown in Fig. 2 rice husk flavone composition suppresses the colony test result of breast cancer cell Colony forming.Rice husk flavones
After composition is incubated MCF-7 cells for a period of time, colony number substantially reduction compared with control group.Concentration is 25mg/ml after 24h
When can substantially reduce breast cancer cell formation colony, concentration be 50mg/ml when colony reduction it is extremely notable.
(10) Transwell is tested
Tumor cell migration experiment is using a diameter of 6.5mm transwell cells, and wherein endoporus size is 8.0 μm.Will
MCF-7 cell serums starvation 24h, then using Trypsin Induced, is counted, and be resuspended with plasma-free DMEM medium.About 1
×105Cell is inoculated into upper strata cell, and bottom chamber adds the DMEM culture mediums containing 10% hyclone.Cell is placed on 37
DEG C culture 24h.After culture, the cell of the small chamber internal surface in upper strata is migrated.Cell through cell is fixed and dyed and aobvious
Counted under micro mirror.Each experimental group do three it is parallel take its average value, as a result as shown in Figure 3.
As seen from Figure 3, an important feature of tumour cell is that they have invasion and move to the ability of remote organ,
This is the main cause of nearly all cancer mortality, using Transwell experimental analysis rice husk flavone compositions to MCF-7 cells
Migration influence.As shown in figure 3, compared with control group, the quantity of rice husk flavone composition treatment group MCF-7 cell transmembranes migration
Decline to a great extent, as a result show that rice husk flavone composition can significantly inhibit MCF-7 cell migrations.
Anti-breast cancer health products of the embodiment 2 containing rice husk flavones
Anti-breast cancer health products containing rice husk flavones, are made up of following raw materials by weight：
Rice husk flavones 60%, cistanche extracts 5%, Fructus Psoraleae extract 10%,
Viscum album extract 5%, Rhizoma Chuanxiong extract 10%, Placenta Hominis extract 10%.
Anti-breast cancer health products of the embodiment 3 containing rice husk flavones
Anti-breast cancer health products containing rice husk flavones, are made up of following raw materials by weight：
Rice husk flavones 50%, cistanche extracts 10%, Fructus Psoraleae extract 10%,
Viscum album extract 10%, Rhizoma Chuanxiong extract 10%, Placenta Hominis extract 10%.
Claims (7)
1. the anti-breast cancer health products containing rice husk flavones, it is characterised in that be made up of following raw materials by weight：
Rice husk flavones 50-70%, cistanche extracts 5-10%, Fructus Psoraleae extract 5-10%,
Viscum album extract 5-10%, Rhizoma Chuanxiong extract 5-10%, Placenta Hominis extract 5-10%.
2. the anti-breast cancer health products as claimed in claim 1 containing rice husk flavones, it is characterised in that：The rice husk flavones
Preparation method is as follows：
1) cellulase degradation is extracted：Solid-liquid ratio in mass ratio 1:20-1:30, rice husk powder is dissolved in water, cellulase is added
Measure as 150-180U/g, carry out hot water extraction, it is 40-60 DEG C control temperature, pH value range 4.0-6.0, and enzymolysis time is 1-3h,
Centrifugation, filtering, obtains that cellulase degradation extract solution is standby, and sediment A proceeds ethanol assisted extraction；
2) ethanol assisted extraction：Solid-liquid ratio in mass ratio 1:10-1:30, sediment A is dissolved in ethanol, volume fraction of ethanol is
50-70%, extraction time is 2-3h, is centrifuged, and filtering obtains that ethanol assisted extraction liquid is standby, sediment B proceeds ultrasonic leaching
Carry；
3) ultrasound extraction：Solid-liquid ratio presses in mass ratio 1:20-1:30, sediment B is dissolved in ethanol, volume fraction of ethanol is 50-
70%, ultrasonic power is 160-200W, and ultrasonic time is 20-40min, and suction filtration obtains ultrasonic extraction extract solution standby；
4) merge cellulase degradation extract solution, ethanol assisted extraction liquid and ultrasound extraction extract solution, total extract solution is rotated and steamed
After hair concentration, filter paper prefiltration, ultrafiltration is carried out with filter membrane, ultrafiltrate sterile cryo is dried, and obtains thick rice husk flavones dry powder.
3. the anti-breast cancer health products as claimed in claim 1 containing rice husk flavones, it is characterised in that：The saline cistanche is extracted
The preparation method of thing：At ambient temperature, the saline cistanche solid powder for being ground into 200 mesh is weighed；Difference in mass ratio 1:10 ratios
Example weighs saline cistanche solid powder and deionized water, ultrasonication 30min, and room temperature extracts 3h, and filter paper prefiltration, milipore filter surpass
Filter, ultrafiltrate sterile cryo is dried, and obtains cistanche extracts.
4. the anti-breast cancer health products as claimed in claim 1 containing rice husk flavones, it is characterised in that：The psoralea corylifolia is extracted
The preparation method of thing：At ambient temperature, the psoralea corylifolia solid powder for being ground into 200 mesh is weighed；Difference in mass ratio 1:10 ratios
Example weighs psoralea corylifolia solid powder and deionized water, ultrasonication 30min, and room temperature extracts 3h, and filter paper prefiltration, milipore filter surpass
Filter, ultrafiltrate sterile cryo is dried, and obtains Fructus Psoraleae extract.
5. the anti-breast cancer health products as claimed in claim 1 containing rice husk flavones, it is characterised in that：The Ramulus Taxilli is extracted
The preparation method of thing：At ambient temperature, the Ramulus Taxilli solid powder for being ground into 200 mesh is weighed；Difference in mass ratio 1:10 ratios
Example weighs Ramulus Taxilli solid powder and deionized water, ultrasonication 30min, and room temperature extracts 3h, and filter paper prefiltration, milipore filter surpass
Filter, ultrafiltrate sterile cryo is dried, and obtains Viscum album extract.
6. the anti-breast cancer health products as claimed in claim 1 containing rice husk flavones, it is characterised in that：The Rhizoma Chuanxiong extract
Preparation method：At ambient temperature, the Ligusticum wallichii solid powder for being ground into 200 mesh is weighed；Difference in mass ratio 1:10 ratios claim
Take Ligusticum wallichii solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltration
Liquid sterile cryo is dried, and obtains Rhizoma Chuanxiong extract.
7. the anti-breast cancer health products as claimed in claim 1 containing rice husk flavones, it is characterised in that：The dried human placenta is extracted
The preparation method of thing：At ambient temperature, the dried human placenta solid powder for being ground into 200 mesh is weighed；Difference in mass ratio 1:10 ratios
Example weighs dried human placenta solid powder and deionized water, ultrasonication 30min, and room temperature extracts 3h, and filter paper prefiltration, milipore filter surpass
Filter, ultrafiltrate sterile cryo is dried, and obtains Placenta Hominis extract.
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Cited By (1)
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| CN109806283A (en) * | 2019-03-21 | 2019-05-28 | 辽宁大学 | A kind of phellinus igniarius mycelium flavones and its preparation method and application |
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