CN107149150A - Anti-lung cancer health products containing rice bran polyphenol - Google Patents
Anti-lung cancer health products containing rice bran polyphenol Download PDFInfo
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- 235000009566 rice Nutrition 0.000 title claims abstract description 63
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- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 58
- 206010058467 Lung neoplasm malignant Diseases 0.000 title claims abstract description 46
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- 235000010674 Prunella vulgaris Nutrition 0.000 claims abstract description 11
- BLUAFEHZUWYNDE-NNWCWBAJSA-N artemisinin Chemical compound C([C@](OO1)(C)O2)C[C@H]3[C@H](C)CC[C@@H]4[C@@]31[C@@H]2OC(=O)[C@@H]4C BLUAFEHZUWYNDE-NNWCWBAJSA-N 0.000 claims abstract description 11
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/282—Artemisia, e.g. wormwood or sagebrush
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/536—Prunella or Brunella (selfheal)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
- A61K36/605—Morus (mulberry)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
- A61K36/748—Oldenlandia or Hedyotis
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8969—Polygonatum (Solomon's seal)
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The present invention discloses a kind of anti-lung cancer health products containing rice bran polyphenol, is made up of following raw materials by weight:Rice bran polyphenol 50 70%, Rhizoma Polygonati extract 5 10%, Prunella vulgaris extract 5 10%, Artemisinin 5 10%, mulberry bark extract 5 10%, herba hedyotis diffusae extract 5 10%.Prepared by abundant raw material of the present invention, easily scientific formula, production, cost is low, and effect is good.The present invention passes through the experimental study to lung carcinoma cell outside human body, it was found that the composition of rice bran polyphenol has good inhibiting effect to tumor cell in vitro, there is the effect of highly significant for the preventing and treating of lung cancer, the toxic side effect that can not only avoid excessive chemical synthetic drug from causing, also causes rice bran polyphenol compositions to have broader development prospect.
Description
Technical field
The invention belongs to field of health care products, a kind of health care containing rice bran polyphenol with anti-lung cancer function is specifically related to
Product.
Background technology
Lung cancer is China or even world's incidence of disease highest malignant tumour, as human cancer major causes of death it
One.The treatment of lung cancer has surgical intervention, radiotherapy, chemotherapy and immunotherapy etc..Most of chemotherapeutics, no matter individually
Or combine, it is not good to lung cancer curative effect, and there is larger side effect, therefore, seek new and Small side effects chemotherapeutics
Thing receives much concern.The developing anti-tumor medicaments from plant, are a focuses of domestic and international anti-cancer agent exploitation.Modern medicine is ground
Studying carefully discovery polyphenol compound has stronger anti-tumor biological, and inside and outside experimental study shows polyphenol compound pair
Lung cancer different phase all has obvious inhibitory action.Chinese medicine is the powerful mean that motherland's medical use carrys out disease preventing and treating, with chemistry
Synthetic drug is compared, in addition to Chinese medicine is except having the advantages that convenient material drawing, cheap and toxic side effect is small, it is often more important that,
Mitigate clinical symptoms, improve life quality, prevent relapse and metastasis, extend life cycle, pre- preventing tumor improves patient's immunity, increases
Strong Radiotherapy chemotherapy sensitiveness, mitigates many aspects such as Radiotherapy chemotherapy adverse reaction and plays an important role.Therefore as social entering
Notice is increasingly turned to crude drug by step, the development of productivity, modern from chemical combination patent medicine, it is desirable to developed from natural drug
Go out safer effective new drug to substitute the former, so as to reduce the generation of medicine source disease.
The content of the invention
Set out, make the present invention seeks to the processing byproduct rice bran for the grass family cereal crops paddy rice planted extensively with China
There is provided a kind of natural health-care products of anti-lung cancer for standby rice bran polyphenol and the mixing of other Chinese medical extracts.
The technical solution adopted by the present invention is:
Anti-lung cancer health products containing rice bran polyphenol, are made up of following raw materials by weight:
Rice bran polyphenol 50-70%, Rhizoma Polygonati extract 5-10%, Prunella vulgaris extract 5-10%,
Artemisinin 5-10%, mulberry bark extract 5-10%, herba hedyotis diffusae extract 5-10%.
The anti-lung cancer health products containing rice bran polyphenol, the preparation method of the rice bran polyphenol is as follows:
1) cellulase degradation is extracted:Solid-liquid ratio in mass ratio 1:10-1:30, rice bran powder is dissolved in water, cellulase
Addition is 120-180U/g, carries out hot water extraction, and it is 50-60 DEG C to control temperature, pH value range 5.0-6.0, and enzymolysis time is
1-2h, is centrifuged, and filtering obtains that cellulase degradation extract solution is standby, sediment A proceeds ethanol assisted extraction;
2) ethanol assisted extraction:Solid-liquid ratio in mass ratio 1:10-1:30, sediment A is dissolved in ethanol, volume fraction of ethanol
For 50-70%, extraction time is 2-3h, is centrifuged, and filtering obtains that ethanol assisted extraction liquid is standby, sediment B proceeds ultrasonic leaching
Carry;
3) ultrasound extraction:Solid-liquid ratio in mass ratio 1:10-1:30, sediment B is dissolved in ethanol, volume fraction of ethanol is
50-70%, ultrasonic power is 160-200W, and ultrasonic time is 20-30min, and suction filtration obtains ultrasonic extraction extract solution standby;
4) merge cellulase degradation extract solution, ethanol assisted extraction liquid and ultrasound extraction extract solution, total extract solution is revolved
Turn to be concentrated by evaporation, after filter paper prefiltration, ultrafiltration is carried out with filter membrane, ultrafiltrate sterile cryo is dried, and obtains brown shorts polyphenol dry powder.
The anti-lung cancer health products containing rice bran polyphenol, the preparation method of the Rhizoma Polygonati extract:At ambient temperature,
Weigh the sealwort solid powder for being ground into 200 mesh;Difference in mass ratio 1:10 ratios weigh sealwort solid powder and deionized water,
Ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate sterile cryo is dried, and obtains sealwort extraction
Thing.
The anti-lung cancer health products containing rice bran polyphenol, the preparation method of the Prunella vulgaris extract:In room temperature condition
Under, weigh the selfheal solid powder for being ground into 200 mesh;Difference in mass ratio 1:10 ratios weigh selfheal solid powder and gone
Ionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate sterile cryo is dried, and obtains the summer
Withered grass extract.
The described anti-lung cancer health products containing rice bran polyphenol, the preparation method of the Artemisinin:In room temperature condition
Under, weigh the sweet wormwood solid powder for being ground into 200 mesh;Difference in mass ratio 1:10 ratios weigh sweet wormwood solid powder and deionization
Water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate sterile cryo is dried, obtains sweet wormwood and carry
Take thing.
The described anti-lung cancer health products containing rice bran polyphenol, the preparation method of described mulberry bark extract:In room temperature
Under the conditions of, weigh the root bark of white mulberry solid powder for being ground into 200 mesh;Difference in mass ratio 1:10 ratios weigh root bark of white mulberry solid powder
And deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, the drying of ultrafiltrate sterile cryo,
Obtain mulberry bark extract.
The described anti-lung cancer health products containing rice bran polyphenol, the preparation method of described herba hedyotis diffusae extract:
Under room temperature condition, the oldenlandia diffusa solid powder for being ground into 200 mesh is weighed;Difference in mass ratio 1:10 ratios weigh long-noded pit viper
Tongue grass solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate
Sterile cryo is dried, and obtains herba hedyotis diffusae extract.
The beneficial effects of the invention are as follows:Using the polyphenol of paddy rice processing byproduct rice bran powder extraction as active material,
Anti-lung cancer health products or medicine are prepared, wide material sources are safe, and production cost is low, and take and carry very convenient,
Absorb quickly and effect is good, therefore possess very broad mass market prospect.
Prepared by abundant raw material of the present invention, easily scientific formula, production, cost is low, and effect is good, and innovation is by rice bran polyphenol
As the core functionality raw material of anti-lung cancer, the Chinese medicine composition for being used to treat lung cancer of proposition, compatibility rationally, is formed with anti-
Polyphenol, the Chinese herbal medicine mixed health product of lung cancer.The present invention has found rice bran polyphenol by the experimental study to lung carcinoma cell outside human body
Composition there is good inhibiting effect to tumor cell in vitro, have the effect of highly significant for the preventing and treating of lung cancer, no
The toxic side effect that can only avoid excessive chemical synthetic drug from causing, also causes rice bran polyphenol compositions to have broader hair
Exhibition prospect.
Brief description of the drawings
Fig. 1 is that rice bran polyphenol compositions influence on the cell growth of human lung carcinoma cell line 973.
Fig. 2 is the colony test result that rice bran polyphenol compositions suppress the formation of 973 cell colonies.
Fig. 3 is that rice bran polyphenol compositions suppress 973 cell migration assay results.
Embodiment
Anti-lung cancer health products of the embodiment 1 containing rice bran polyphenol
(1) preparation of rice bran polyphenol
Rice bran polyphenol is extracted using the process combination of cellulase degradation-alcohol steep-ultrasonic wave method of fractional steps, step is such as
Under:
1) cellulase degradation:Rice bran is gone into the removal of impurity, dried, size-reduced machine is crushed, 60 mesh sieves is crossed, bran powder is made
End.Solid-liquid ratio in mass ratio 1:20, rice bran powder is dissolved in water, cellulase addition is 150U/g, carries out hot water extraction, control
Temperature processed is 50 DEG C, pH 5.0, and enzymolysis time is 2h, and 4000r/min carries out centrifugation 10min, and filtering obtains cellulase degradation and carried
Take liquid standby, sediment A proceeds alcohol steep;
2) ethanol assisted extraction:Solid-liquid ratio in mass ratio 1:20, sediment A is dissolved in ethanol, volume fraction of ethanol is
60%, extraction time is 2h, is centrifuged, and filtering obtains that ethanol assisted extraction liquid is standby, sediment B proceeds ultrasonic extraction;
3) ultrasound extraction:Solid-liquid ratio in mass ratio 1:20, sediment B is dissolved in ethanol, volume fraction of ethanol is 60%, surpassed
Acoustic power is 160W, and ultrasonic time is 30min, and suction filtration obtains ultrasonic extraction extract solution standby;
4) cellulase degradation extract solution, ethanol assisted extraction liquid and ultrasound extraction extract solution are merged, extract solution rotation is steamed
After hair concentration, filter paper prefiltration, ultrafiltration is carried out with filter membrane, ultrafiltrate sterile cryo is dried, and obtains brown shorts polyphenol dry powder.
(2) preparation of Rhizoma Polygonati extract
At ambient temperature, the sealwort solid powder for being ground into 200 mesh is weighed;In mass ratio 1:10 ratios weigh sealwort and consolidated
Body powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate are sterile cold
It is lyophilized dry, obtain Rhizoma Polygonati extract.
(3) preparation of Prunella vulgaris extract
At ambient temperature, the selfheal solid powder for being ground into 200 mesh is weighed;In mass ratio 1:It is withered that 10 ratios weigh the summer
Careless solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate without
Bacterium is freeze-dried, and obtains Prunella vulgaris extract.
(4) preparation of Artemisinin
At ambient temperature, the sweet wormwood solid powder for being ground into 200 mesh is weighed;In mass ratio 1:10 ratios weigh sweet wormwood and consolidated
Body powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate are sterile cold
It is lyophilized dry, obtain Artemisinin.
(5) preparation of mulberry bark extract
At ambient temperature, the root bark of white mulberry solid powder for being ground into 200 mesh is weighed;In mass ratio 1:10 ratios weigh Sang Bai
Skin solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate without
Bacterium is freeze-dried, and obtains mulberry bark extract.
(6) preparation of herba hedyotis diffusae extract
At ambient temperature, the oldenlandia diffusa solid powder for being ground into 200 mesh is weighed;In mass ratio 1:10 ratios are weighed
Oldenlandia diffusa solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration,
Ultrafiltrate sterile cryo is dried, and obtains herba hedyotis diffusae extract.
(7) anti-lung cancer Halth-care composition (abbreviation rice bran polyphenol compositions) containing rice bran polyphenol
It is made up of following raw materials by weight:
Rice bran polyphenol 60%, Rhizoma Polygonati extract 5%, Prunella vulgaris extract 10%,
Artemisinin 5%, mulberry bark extract 10%, herba hedyotis diffusae extract 10%.
(8) MTT experiment
The present embodiment uses the cell of human lung carcinoma cell line 973, detects that the external anticancer of rice bran polyphenol compositions is lived using mtt assay
Property, experimental procedure is as follows:
1) lung cancer cell line (973) cell in growth period of taking the logarithm is inoculated in 96 orifice plates, and incubated overnight makes its adherent;
2) concentration (6.25,12.5 and 25mg/ml) of rice bran polyphenol compositions;
3) MTT (5mg/ml) is added after 24h;
4) and then 96 orifice plates are placed in 37 DEG C of constant incubators and continue to cultivate 4h;
5) after processing terminates, MTT is suctioned out, 150 μ l DMSO is added, is completely dissolved first a ceremonial jade-ladle, used in libation crystal;
6) increment of 973 cells is assessed by measuring absorbance of each hole at 490nm;As a result it is as shown in Figure 1.
As shown in figure 1, the rice bran polyphenol compositions (6.25,12.5 and 25mg/ml) of various concentrations are incubated 973 cells respectively
24h, then utilizes MTT colorimetric determinations.As a result show, after handling lung carcinoma cell for a period of time, concentration is 12.5,25mg/ml
Rice bran polyphenol compositions show significant inhibitory action, concentration is that 25mg/ml group inhibiting rates reach after 24h
79.5%, as a result show, rice bran polyphenol compositions can suppress human lung carcinoma cell growth in vitro.
(9) Colony forming is tested
The lung carcinoma cell of different treatment groups is inoculated on orifice plate with the density of every 1000 cells in hole, and overnight incubation makes it
It is adherent.12.5 and 25mg/ml rice bran polyphenol compositions culture 10d is separately added into, culture medium is suctioned out, is carefully washed with PBS per hole
Twice.Colony is fixed into 20min using methanol, then with 0.5% violet staining 15min, is rinsed and taken pictures with clear water, as a result
As shown in Figure 2.
As shown in Fig. 2 rice bran polyphenol compositions suppress the colony test result of lung carcinoma cell Colony forming.Rice bran polyphenol group
After compound is incubated 973 cells for a period of time, colony number substantially reduction compared with control group.When concentration is 12.5mg/ml after 24h
The colony of lung carcinoma cell formation can be reduced, the reduction of colony is extremely notable when concentration is 25mg/ml.
(10) Transwell is tested
Tumor cell migration experiment is using a diameter of 6.5mm transwell cells, and wherein endoporus size is 8.0 μm.Will
Lung carcinoma cell serum starvation 24h, then using Trypsin Induced, is counted, and be resuspended with the culture medium of serum-free 1640.About 1
×105Cell is inoculated into upper strata cell, and bottom chamber adds 1640 culture mediums containing 10% hyclone.Cell is placed on 37
DEG C culture 24h.After culture, the cell of the small chamber internal surface in upper strata is migrated.Cell through cell is fixed and dyed and aobvious
Counted under micro mirror.Each experimental group do three it is parallel take its average value, as a result as shown in Figure 3.
As seen from Figure 3, an important feature of tumour cell is that they have invasion and move to the ability of remote organ,
This is the main cause of nearly all cancer mortality, using Transwell experimental analysis rice bran polyphenol compositions to 973 cells
Migration influence.As shown in figure 3, compared with control group, the quantity of the cell transmembrane of rice bran polyphenol compositions treatment group 973 migration is significantly
Decline, as a result show that rice bran polyphenol compositions can significantly inhibit 973 cell migrations.
Anti-lung cancer health products of the embodiment 2 containing rice bran polyphenol
Anti-lung cancer health products containing rice bran polyphenol, are made up of following raw materials by weight:
Rice bran polyphenol 70%, Rhizoma Polygonati extract 10%, Prunella vulgaris extract 5%,
Artemisinin 5%, mulberry bark extract 5%, herba hedyotis diffusae extract 5%.
Anti-lung cancer health products of the embodiment 3 containing rice bran polyphenol
Anti-lung cancer health products containing rice bran polyphenol, are made up of following raw materials by weight:
Rice bran polyphenol 50%, Rhizoma Polygonati extract 10%, Prunella vulgaris extract 10%,
Artemisinin 10%, mulberry bark extract 10%, herba hedyotis diffusae extract 10%.
Claims (7)
1. the anti-lung cancer health products containing rice bran polyphenol, it is characterised in that be made up of following raw materials by weight:
Rice bran polyphenol 50-70%, Rhizoma Polygonati extract 5-10%, Prunella vulgaris extract 5-10%,
Artemisinin 5-10%, mulberry bark extract 5-10%, herba hedyotis diffusae extract 5-10%.
2. the anti-lung cancer health products as claimed in claim 1 containing rice bran polyphenol, it is characterised in that:The preparation of the rice bran polyphenol
Method is as follows:
1) cellulase degradation is extracted:Solid-liquid ratio in mass ratio 1:10-1:30, rice bran powder is dissolved in water, cellulase is added
Measure as 120-180U/g, carry out hot water extraction, it is 50-60 DEG C control temperature, pH value range 5.0-6.0, and enzymolysis time is 1-2h,
Centrifugation, filtering, obtains that cellulase degradation extract solution is standby, and sediment A proceeds ethanol assisted extraction;
2) ethanol assisted extraction:Solid-liquid ratio in mass ratio 1:10-1:30, sediment A is dissolved in ethanol, volume fraction of ethanol is
50-70%, extraction time is 2-3h, is centrifuged, and filtering obtains that ethanol assisted extraction liquid is standby, sediment B proceeds ultrasonic leaching
Carry;
3) ultrasound extraction:Solid-liquid ratio in mass ratio 1:10-1:30, sediment B is dissolved in ethanol, volume fraction of ethanol is 50-
70%, ultrasonic power is 160-200W, and ultrasonic time is 20-30min, and suction filtration obtains ultrasonic extraction extract solution standby;
4) merge cellulase degradation extract solution, ethanol assisted extraction liquid and ultrasound extraction extract solution, total extract solution is rotated and steamed
After hair concentration, filter paper prefiltration, ultrafiltration is carried out with filter membrane, ultrafiltrate sterile cryo is dried, and obtains brown shorts polyphenol dry powder.
3. the anti-lung cancer health products as claimed in claim 1 containing rice bran polyphenol, it is characterised in that:The system of the Rhizoma Polygonati extract
Preparation Method:At ambient temperature, the sealwort solid powder for being ground into 200 mesh is weighed;Difference in mass ratio 1:10 ratios weigh Huang
Smart solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate without
Bacterium is freeze-dried, and obtains Rhizoma Polygonati extract.
4. the anti-lung cancer health products as claimed in claim 1 containing rice bran polyphenol, it is characterised in that:The Prunella vulgaris extract
Preparation method:At ambient temperature, the selfheal solid powder for being ground into 200 mesh is weighed;Difference in mass ratio 1:10 ratios claim
Selfheal solid powder and deionized water, ultrasonication 30min are taken, room temperature extracts 3h, and filter paper prefiltration, milipore filter ultrafiltration surpass
Filtrate sterile cryo is dried, and obtains Prunella vulgaris extract.
5. the anti-lung cancer health products as claimed in claim 1 containing rice bran polyphenol, it is characterised in that:The Artemisinin
Preparation method:At ambient temperature, the sweet wormwood solid powder for being ground into 200 mesh is weighed;Difference in mass ratio 1:10 ratios are weighed
Sweet wormwood solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, filter paper prefiltration, milipore filter ultrafiltration, ultrafiltrate
Sterile cryo is dried, and obtains Artemisinin.
6. the anti-lung cancer health products as claimed in claim 1 containing rice bran polyphenol, it is characterised in that:The described root bark of white mulberry is extracted
The preparation method of thing:At ambient temperature, the root bark of white mulberry solid powder for being ground into 200 mesh is weighed;Difference in mass ratio 1:10 ratios
Example weighs root bark of white mulberry solid powder and deionized water, ultrasonication 30min, and room temperature extracts 3h, and filter paper prefiltration, milipore filter surpass
Filter, ultrafiltrate sterile cryo is dried, and obtains mulberry bark extract.
7. the anti-lung cancer health products as claimed in claim 1 containing rice bran polyphenol, it is characterised in that:Described oldenlandia diffusa
The preparation method of extract:At ambient temperature, the oldenlandia diffusa solid powder for being ground into 200 mesh is weighed;Quality is pressed respectively
Than 1:10 ratios weigh oldenlandia diffusa solid powder and deionized water, ultrasonication 30min, room temperature extraction 3h, the first mistake of filter paper
Filter, milipore filter ultrafiltration, ultrafiltrate sterile cryo are dried, and obtain herba hedyotis diffusae extract.
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