CN107142296B - Method for efficiently producing ethanol from kitchen waste - Google Patents
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- CN107142296B CN107142296B CN201710414934.3A CN201710414934A CN107142296B CN 107142296 B CN107142296 B CN 107142296B CN 201710414934 A CN201710414934 A CN 201710414934A CN 107142296 B CN107142296 B CN 107142296B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
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- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/56—Lactic acid
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Abstract
The invention belongs to the technical field of kitchen waste treatment, and particularly discloses a method for efficiently producing ethanol by treating kitchen waste. The method comprises the following steps: s1, pretreatment: carrying out hydrothermal pretreatment on the sorted kitchen waste, and then separating solid kitchen waste; s2, ethanol fermentation: and (4) adding ethanol fermentation bacteria into the solid kitchen waste separated in the S1, and separating after fermentation to obtain ethanol. The method provided by the invention greatly improves the product yield, has short fermentation time and practical application value, and is very suitable for industrial application.
Description
Technical Field
The invention relates to the technical field of kitchen waste treatment, in particular to a method for efficiently producing ethanol from kitchen waste.
Background
Kitchen waste, commonly known as swill, also known as swill and hogwash, is a domestic waste formed in the process of domestic consumption of residents, is extremely easy to rot and deteriorate, emits foul smell, and spreads bacteria and viruses, the main components of the kitchen waste comprise rice and flour food residues, vegetables, animal and vegetable oil, meat and bones and the like, and the kitchen waste comprises starch, cellulose, protein, lipid, inorganic salt and other components in terms of chemical composition. With the rapid development of economy in China, the yield of kitchen waste is larger and larger, and the research on the recycling of the kitchen waste is more and more; the method for producing the fuel ethanol and the lactic acid by utilizing the kitchen waste is an effective way for realizing high-value utilization of the fuel ethanol and the lactic acid.
The chinese patent with application number 201310437655.0 discloses a method for promoting the production of ethanol and lactic acid from kitchen waste by hydrothermal treatment, namely, the kitchen waste is subjected to hydrothermal pretreatment before fermentation, the yield of the ethanol obtained by the method is low, and the utilization rate of the kitchen waste is low. Therefore, a treatment method with high ethanol yield and high utilization rate of the kitchen waste still needs to be researched.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide a method for efficiently producing ethanol by using kitchen waste.
The purpose of the invention is realized by the following technical scheme:
a method for efficiently producing ethanol by using kitchen waste comprises the following steps:
s1, pretreatment: carrying out hydrothermal pretreatment on the kitchen waste, and then separating solid kitchen waste;
s2, ethanol fermentation: adding ethanol fermentation bacteria into the solid kitchen waste separated in the S1, and separating after fermentation to obtain ethanol;
wherein, the ethanol fermentation bacteria of S2 is added by two times, and the adding sequence is as follows: adding at least bacillus (B)Bacillus) And yeasts (Saccharomyces) The ethanol fermentation flora 1 is added with at least ruminococcus (A)Ruminococcus) Amino acid bacterium (A), (B), (C)Aminobacterium) Aspergillus (a), (b)Aspergillus) And yeasts (Saccharomyces) The ethanol fermentation flora 2.
In the ethanol fermentation process, the inventor finds that if the bacteria for ethanol fermentation are added into the solid kitchen waste in batches, the ethanol obtained by fermentation has higher yield. Especially when Bacillus (B) is addedBacillus) And yeasts (Saccharomyces) The ethanol fermentation flora 1 is added with rumen coccus (A) after being fermented for a plurality of timesRuminococcus) Amino acid bacterium (A), (B), (C)Aminobacterium) Aspergillus (a), (b)Aspergillus) And yeasts (Saccharomyces) The ethanol fermentation flora 2 has the highest ethanol yield by adopting the addition sequence for fermentation.
Preferably, the ethanol fermentation bacteria of S2 further comprises Lactobacillus（Lactobacillus) Leuconostoc (Leuconostoc sp.)Leuconostoc) Rhizopus and rhizopus (Rhizopus) Lactococcus (A) and (B)Lactococcus) Clostridium (I), (II)Clostridiisalibacter）、A55 D21Comamonas (a)Comamonas) Spirobacteria (A)Spira) Klebsiella bacterium (C.), (Klebsiella) Saccule bacterium (A), (B), (C)Ascosphaera) Vibrio acetobacter (Acetivibrio) Alcaligenes, Alcaligenes (II) and (III)Alcaligenes) Bacteroides (A), (B)Bacteroides) Violomonas bacterium (I), (II)Porphyromonadaceae) Or Pseudomonas bacteria (Pseudomonas) One or more of them.
The main purpose of the kitchen waste pretreatment is to hydrolyze macromolecular substances such as cellulose, starch, protein and the like in the kitchen waste into small molecules, so that the subsequent microbial fermentation is convenient for producing ethanol and lactic acid. The hydrothermal treatment is to perform thermochemical treatment on the kitchen waste in the presence of water, so that the diffusion coefficient of solid particles can be increased, organic matters are further decomposed through heat conduction, and the reaction time is shortened.
Preferably, in S1, the pretreatment includes sequentially subjecting the sorted kitchen waste to ultrasonic treatment, hydrothermal treatment and microwave treatment, and then separating solid kitchen waste. Firstly, carrying out ultrasonic treatment on the kitchen waste, and enabling solid particles to be violently impacted under the mechanical action of the kitchen waste, so that the formed cavitation action promotes the dispersion of the solid; in addition, when the ultrasonic waves are absorbed by the solid particles, a large amount of heat is generated, so that the temperature of the kitchen waste is suddenly increased, C-C bonds are broken, macromolecular hydrocarbon is decomposed into micromolecular hydrocarbon, and the hydrolysis of the kitchen waste is promoted. Microwave treatment can lead the small molecular substances to be directionally catalyzed and cracked, and promote hydrolysis reaction. After the kitchen waste is subjected to ultrasonic wave, hydrothermal treatment and microwave pretreatment in sequence, the hydrolysis effect of the kitchen waste can be obviously improved, and the yield of a subsequent fermentation product of the kitchen waste is effectively improved.
Preferably, the ultrasonic treatment conditions are as follows: the ultrasonic power is 15-1500 w/L, and the processing time is 5-60 min.
Preferably, the hydrothermal treatment conditions are: the hydrothermal temperature is 90-300 ℃, and the treatment time is 5-150 min.
Preferably, the microwave treatment conditions are as follows: the microwave power is 100-2500 kw, the time is 2-120 min, and the temperature is 90-300 ℃.
The ultrasonic treatment, the hydrothermal treatment and the microwave treatment can be sequentially carried out in an integrated device provided with an ultrasonic generator, a hydrothermal device and a microwave generator, and also can be sequentially carried out in an ultrasonic reactor, a hydrothermal device and a microwave reactor respectively.
Preferably, the addition amount of the ethanol fermentation bacteria of S2 is 0.01-20% (volume ratio), and the fermentation time is 1-25 days.
More preferably, the S2 is added with an enzyme for ethanol fermentation, the total addition amount of the enzyme for ethanol fermentation is 0.01-15% (volume ratio), and the enzymolysis time is 1-120 hours.
Preferably, the enzyme for ethanol fermentation in S2 is selected from one or more of alcohol dehydrogenase, acetaldehyde dehydrogenase, pyruvate carboxylase, amylase, cellulase, hemicellulase, ligninase, lipase, protease, saccharifying enzyme, pectinase, xylanase or β -glucanase.
Preferably, before S1, the kitchen waste is sorted to remove impurities which cannot be fermented in the kitchen waste.
In the invention, acid or/and alkali pretreatment is carried out before ethanol fermentation, so that the hydrolysis rate of the kitchen waste can be increased, and the yield of the product ethanol is further improved. Therefore, the kitchen waste is preferably subjected to acid or/and alkali pretreatment before the ethanol fermentation.
In the invention, S1, the solid kitchen waste is separated through a three-phase separation operation, the three-phase separation is to discharge the kitchen waste in a spray explosion mode, stand to remove the upper layer of grease to obtain the solid kitchen waste, and discharge the kitchen waste after pretreatment in the spray explosion mode, so that the grease in the kitchen waste can be separated from the solid and water to the maximum extent, and the separation amount of the floatable oil is increased.
Preferably, in S2, lactic acid fermenting bacteria are added to the fermentation residue obtained after separating ethanol to produceLactic acid; more preferably, the lactic acid fermenting bacteria include at least lactobacillus (b)Lactobacillus）。
Most preferably, the lactic acid fermenting bacteria further comprise Streptococcus (S.sp.) (Streptococcus) Bifidobacterium (b), (c), (d), (Bifidobacterium) Bacillus bacteria (b), (b)Bacillus) Lactococcus (A) and (B)Lactococcus) Clostridium (I), (II)Clostridiisalibacter) Rumen coccus (A)Ruminococcus）、A55_D21Spirochete (Spirochaeta), aminobacillus (A)Aminobacterium) Saccule bacterium (A), (B), (C)Ascosphaera) Aspergillus (a), (b)Aspergillus) Rhizopus and rhizopus (Rhizopus) Yeast, (b) yeastSaccharomyces) Vibrio acetobacter (Acetivibrio) Alcaligenes, Alcaligenes (II) and (III)Alcaligenes) Bacteroides (A), (B)Bacteroides) Violomonas bacterium (I), (II)Porphyromonadaceae) Or Pseudomonas bacteria (Pseudomonas) One or more of (a).
Preferably, the addition amount of the lactic acid fermentation bacteria is 0.01-20% (volume ratio), and the fermentation time is 1-30 days.
More preferably, the lactic acid fermentation stage is further added with lactic acid fermentation enzyme, the addition amount of the lactic acid fermentation enzyme is 0.01-15% (volume ratio), and the enzymolysis time is 1-144 hours.
Preferably, the enzyme for lactic acid fermentation is selected from one or more of cellulase, hemicellulase, ligninase, lipase, amylase, protease, β -glucosidase and lactate dehydrogenase.
Preferably, an electric field is applied to carry out electric treatment in the ethanol fermentation and/or lactic acid fermentation processes. More preferably, the voltage of the electric field is 0.1-12V, and the processing time is 6-216 h. The electric field is used for acting on the microbial fermentation, so that the metabolism of the microbes can be stimulated, and the yield of the target product ethanol and/or lactic acid is improved.
In the invention, the temperature in the ethanol fermentation process is 20-80 ℃, and the temperature in the lactic acid fermentation process is 18-80 ℃.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a method for efficiently producing ethanol by kitchen waste, which is to perform hydrothermal pretreatment on sorted kitchen waste and then add microorganisms into solid kitchen waste for ethanol fermentation. Meanwhile, the invention also provides a three-step pretreatment mode, namely, ultrasonic waves, hydrothermal treatment and microwave treatment are adopted to carry out pretreatment on the kitchen waste in sequence, so that the kitchen waste is hydrolyzed more thoroughly, and then high-efficiency microorganisms are adopted to carry out ethanol fermentation; greatly improves the yield of the product ethanol, has short fermentation time, has great popularization and application value, and is very suitable for industrialized application.
Detailed Description
The following examples are presented to further illustrate the present invention and should not be construed as limiting the invention. It is within the scope of the present invention to make simple modifications or alterations to the methods, procedures or conditions of the present invention without departing from the spirit and substance of the invention; unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art.
Example 1
A method for treating kitchen waste to efficiently produce ethanol comprises the following steps:
s1, sorting: removing impurities such as chopsticks and plastics which cannot be used for fermentation in the kitchen waste;
s2, pretreatment: carrying out hydrothermal pretreatment on the sorted kitchen waste; the temperature of the hydrothermal treatment is 90 ℃, and the treatment time is 150 min;
s3, three-phase separation: discharging the kitchen waste in a spray explosion mode, standing, and removing upper-layer grease to obtain solid kitchen waste;
s4, ethanol fermentation: sequentially adding ethanol fermentation flora 1[ bacillus (Bacillus) to the solid kitchen waste separated from the S3Bacillus) And yeasts (Saccharomyces）]Fermenting at 35 deg.C for 12 h; then adding ethanol fermentation flora 2[ ruminococcus: (A), (B) and (C)Ruminococcus) Amino acid bacterium (A), (B), (C)Aminobacterium) Aspergillus (a), (b)Aspergillus) And yeasts (Saccharomyces）]Fermenting at 35 deg.C for 216h, and extracting ethanol from the fermentation broth.
In this example, the ethanol fermentation bacteria were added in an amount of 5% (by volume).
Example 2
A method for treating kitchen waste to efficiently produce ethanol comprises the following steps:
s1, sorting: removing impurities such as chopsticks and plastics which cannot be used for fermentation in the kitchen waste;
s2, pretreatment: carrying out hydrothermal pretreatment on the sorted kitchen waste; the temperature of the hydrothermal treatment is 180 ℃, and the treatment time is 80 min;
s3, three-phase separation: discharging the kitchen waste in a spray explosion mode, standing, and removing upper-layer grease to obtain solid kitchen waste;
s4, ethanol fermentation: sequentially adding ethanol fermentation flora 1[ bacillus (Bacillus) to the solid kitchen waste separated from the S3Bacillus) And yeast (Saccharomyces)]Fermenting at 35 deg.C for 12 h; adding cellulase for enzymolysis at 35 deg.C for 12 hr, and adding ethanol fermentation flora 2[ Ruminococcus: (A)Ruminococcus) Amino acid bacterium (A), (B), (C)Aminobacterium) Aspergillus (Aspergillus) and yeast (Saccharomyces)]Fermenting at 35 deg.C for 216h, and extracting ethanol from the fermentation broth;
s5 lactic acid fermentation: adding lipase into the ethanol fermentation residue, performing enzymolysis at 35 deg.C for 12 hr, and adding lactobacillus (Lactobacillus)Lactobacillus）]Fermenting at 35 deg.C for 12h to extract lactic acid from the fermentation broth.
In this example, the amount of the ethanol fermentation bacteria was 5% (by volume), and the amount of the ethanol fermentation enzyme was 2% (by volume). The addition amount of the lactic acid fermentation bacteria is 8% (volume ratio), and the addition amount of the lactic acid fermentation enzyme is 1% (volume ratio).
Example 3
A method for treating kitchen waste to efficiently produce ethanol comprises the following steps:
s1, sorting: removing impurities such as chopsticks and plastics which cannot be used for fermentation in the kitchen waste; then, sequentially adding hydrochloric acid and sodium hydroxide into the kitchen waste to ensure that the volume concentration of the hydrochloric acid and the volume concentration of the sodium hydroxide in the kitchen waste are respectively 1% and 0.5%;
s2, three-step pretreatment: sequentially carrying out ultrasonic treatment, hydrothermal treatment and microwave treatment on the kitchen waste treated by the S1; wherein the power of the ultrasonic wave is 900W/L, and the processing time is 20 min; the temperature of the hydrothermal treatment is 170 ℃, and the treatment time is 80 min; the power of microwave treatment is 900 kw, the temperature is maintained at 100 ℃, and the treatment time is 15 min; the three steps of pretreatment are respectively carried out in an ultrasonic reactor, a hydrothermal device and a microwave reactor in sequence;
s3, three-phase separation: discharging the kitchen waste in a spray explosion mode, standing, and removing upper-layer grease to obtain solid kitchen waste;
s4, ethanol fermentation: adding a mixture of cellulase and amylase into the solid kitchen waste obtained in the step S3 for enzymolysis for 2 hours, and then adding a bacterium [ Comamonas comamonas ] (Comamonas) Spirobacteria (A)Spira) Klebsiella bacterium (C.), (Klebsiella) Amino acid bacterium (A), (B), (C)Aminobacterium) Bacillus bacteria (b), (b)Bacillus) Lactobacillus, Lactobacillus (II)Lactobacillus) Leuconostoc (Leuconostoc sp.)Leuconostoc) Rhizopus (Rhizopus) and yeast (Saccharomyces)]Fermenting at 35 deg.C for 168 hr, and adding ethanol fermenting bacteria (Ruminococcus: (A) to obtain fermented productRuminococcus) Amino acid bacterium (A), (B), (C)Aminobacterium) Yeast (Saccharomyces), aspergillus (Aspergillus), (Aspergillus, etc.)Aspergillus) Rhizopus and rhizopus (Rhizopus) Comamonas (a)Comamonas) And Spirobacteria (A), (B)Spira）]Fermenting at 35 deg.C for 240 hr, and extracting ethanol from the fermentation broth;
s5 lactic acid fermentation: adding amylase and cellulase into ethanol fermentation residue, performing enzymolysis at 30 deg.C for 168 hr, and adding lactobacillus (Lactobacillus)Lactobacillus) Clostridium (I), (II)Clostridiisalibacter) Rumen coccus (A)Ruminococcus) AndA55-D21]fermenting at 30 deg.C for 2 hr, and adding lactic acid fermenting bacteria (Porphyromonas: (B))Porphyromonadaceae）]Fermenting at 30 deg.C for 384 h to extract lactic acid from the fermentation broth.
In this example, the amount of the ethanol fermentation bacteria added was 0.01% (by volume), and the amount of the ethanol fermentation enzyme added was 0.1% (by volume). The addition amount of the lactic acid fermentation bacteria was 0.01% (volume ratio), and the addition amount of the lactic acid fermentation enzyme was 0.1% (volume ratio).
Example 4
The method provided by the embodiment is the same as the embodiment 2, except that an electric field is switched in to perform electric treatment in the S4 ethanol fermentation stage, the voltage of the electric field is 0.1V, and the treatment time is 216 h. And (3) in the lactic acid fermentation stage of S5, switching in an electric field for electric treatment, wherein the voltage of the electric field is 12V, and the treatment time is 72 h.
In this example, the amount of the ethanol fermentation bacteria was 20% (by volume), and the amount of the ethanol fermentation enzyme was 0.1% (by volume). The addition amount of the lactic acid fermentation bacteria is 20% (volume ratio), and the addition amount of the lactic acid fermentation enzyme is 15% (volume ratio).
Example 5
The method provided by this embodiment is the same as embodiment 2, except that the pretreatment in S2 of this embodiment is performed by the following steps: sequentially carrying out ultrasonic treatment, hydrothermal treatment and microwave treatment on the kitchen waste treated by the S1; wherein the power of the ultrasonic wave is 15W/L, and the processing time is 5 min; the temperature of the hydrothermal treatment is 90 ℃, and the treatment time is 5 min; the microwave treatment power is 100kw, the temperature is maintained at 90 deg.C, and the treatment time is 2 min.
In this example, the amount of the ethanol fermentation bacteria was 5% (by volume), and the amount of the ethanol fermentation enzyme was 2% (by volume). The addition amount of the lactic acid fermentation bacteria is 8% (volume ratio), and the addition amount of the lactic acid fermentation enzyme is 1% (volume ratio).
Example 6
The method provided in this embodiment is the same as embodiment 2, except that the three-step pretreatment described in S2 in this embodiment is performed according to the following steps: sequentially carrying out ultrasonic treatment, hydrothermal treatment and microwave treatment on the kitchen waste treated by the S1; wherein the power of the ultrasonic wave is 1500W/L, and the processing time is 60 min; the temperature of the hydrothermal treatment is 250 ℃, and the treatment time is 150 min; the microwave treatment power is 2500 kw, the temperature is maintained at 250 deg.C, and the treatment time is 120 min.
In this example, the amount of the ethanol fermentation bacteria added was 20% (by volume), and the amount of the ethanol fermentation enzyme added was 0.1%. The addition amount of the lactic acid fermentation bacteria is 20% (volume ratio), and the addition amount of the lactic acid fermentation enzyme is 15% (volume ratio).
Example 7
The method provided by this example is the same as example 2, except that no enzyme is added in the S4 ethanol fermentation, the ethanol fermentation flora 1 is added first and fermented for 12h at 35 ℃, and then the ethanol fermentation flora 2 is added and fermented for 216h at 35 ℃. Wherein the strains of the ethanol fermentation flora 1 are increased by lactococcus (lactococcus) compared with the strains of the example 2Lactococcus) Comamonas (a)Comamonas) Klebsiella bacterium (C.), (Klebsiella) Amino acid bacterium (A), (B), (C)Aminobacterium) And Aspergillus (Aspergillus), the species of ethanol fermentation flora 2 increased by Clostridium (Clostridium:) than that of example 2Clostridiisalibacter）、A55-D21Comamonas (a)Comamonas) Spirobacteria (A)Spira) And saccule bacterium (A), (B), (C)Ascosphaera). Bifidobacterium is added to lactic acid fermentation bacteria in S5 lactic acid fermentationBifidobacterium) Aspergillus (a), (b)Aspergillus) Rhizopus and rhizopus (Rhizopus) And yeasts (Saccharomyces). The total amount of each of the above-mentioned bacterial colonies and the fermentation time were the same as in example 2.
Comparative example 1
The method provided by the comparative example is the same as that in example 2, except that in the ethanol fermentation process of S4 in the comparative example, the selected ethanol fermentation bacteria are pichia pastoris, zymomonas mobilis, aspergillus niger, rhizopus tokyo, saccharomyces cerevisiae and trichoderma reesei.
Comparative example 2
The method provided in this comparative example is the same as that of example 2, except that in the ethanol fermentation process of S4 in this comparative example, the ethanol fermentation bacterium group 1 and the bacterium group 2 were added simultaneously after the addition of the enzyme for ethanol fermentation, the total amount of the ethanol fermentation bacteria was the same as that in example 2, and the fermentation time was 240 hours.
The concentrations of ethanol and lactic acid obtained by the fermentation processes provided in examples 1-5 and comparative examples 1-2 are shown in Table 1. As can be seen from table 1, the method provided by the invention adds the bacteria for ethanol fermentation into the solid kitchen waste in several times during the ethanol fermentation process, thereby significantly improving the ethanol yield; meanwhile, the subsequent lactic acid fermentation is facilitated. As is clear from Table 1, the lactic acid yield obtained in the lactic acid fermentation was also high.
TABLE 1 concentrations of ethanol and lactic acid fermented by the methods provided in examples 1-5 and comparative examples 1-2
Claims (9)
1. The method for efficiently producing ethanol by using kitchen waste is characterized by comprising the following steps:
s1, pretreatment: carrying out hydrothermal pretreatment on the sorted kitchen waste, and then separating solid kitchen waste;
s2, ethanol fermentation: adding ethanol fermentation bacteria into the solid kitchen waste separated in the S1, and separating after fermentation to obtain ethanol;
wherein, the ethanol fermentation bacteria of S2 is added by two times, and the adding sequence is as follows: the ethanol fermentation flora 1 including Bacillus (Bacillus) and yeast (Saccharomyces) is added, and then the ethanol fermentation flora 2 including Ruminococcus (Ruminococcus), aminobacillus (aminobacillus), Aspergillus (Aspergillus) and yeast (Saccharomyces) is added.
2. The method according to claim 1, characterized in that the kitchen waste is sorted and sundries are removed before S1; and S1, the pretreatment is to sequentially carry out ultrasonic, hydrothermal and microwave pretreatment on the sorted kitchen waste and then separate solid kitchen waste.
3. The method according to claim 1, wherein the total addition amount of the ethanol fermentation bacteria S2 is 0.01-20%, and the fermentation time is 1-25 days; and S2, ethanol fermentation enzyme is added, the total addition amount of the ethanol fermentation enzyme is 0.01-15%, and the enzymolysis time is 1-120 hours.
4. The method of claim 1, wherein the kitchen waste is pre-treated with acid and/or alkali before being subjected to hydrothermal pretreatment after sorting at S1.
5. The method according to claim 1, wherein lactic acid-fermenting bacteria are added to the residue obtained after separation of ethanol in S2 to produce lactic acid.
6. The method according to claim 5, wherein the lactic acid fermenting bacteria are added in an amount of 0.01 to 20% for 1 to 30 days; and in the lactic acid fermentation stage, an enzyme for lactic acid fermentation is added, the addition amount of the enzyme for lactic acid fermentation is 0.01-15%, and the enzymolysis time is 1-144 hours.
7. The method according to any one of claims 1 to 6, wherein the hydrothermal treatment conditions of S1 are: the hydrothermal temperature is 90-300 ℃, and the treatment time is 5-150 min.
8. The method according to any one of claims 1 to 6, wherein an electric field is applied to perform the electrical treatment during the ethanol fermentation process.
9. The method according to any one of claims 5 to 6, wherein an electric field is applied to perform the electrical treatment during the lactic acid fermentation process.
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