CN107119102B - Method for efficiently producing lactic acid by utilizing kitchen waste - Google Patents
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Abstract
The invention belongs to the technical field of kitchen waste treatment, and particularly discloses a method for efficiently producing lactic acid by utilizing kitchen waste. The method comprises the following steps: s1, pretreatment: carrying out hydrothermal pretreatment on the kitchen waste, and then separating solid kitchen waste; s2, lactic acid fermentation: adding lactic acid fermentation bacteria into the solid kitchen waste separated in the S1, and separating to obtain lactic acid after fermentation. The method provided by the invention greatly improves the product yield, has short fermentation time and practical application value, and is very suitable for industrial application.
Description
Technical Field
The invention relates to the technical field of kitchen waste treatment, in particular to a method for efficiently producing lactic acid by utilizing kitchen waste.
Background
Kitchen waste, commonly known as swill, also known as swill and hogwash, is a domestic waste formed in the process of domestic consumption of residents, is extremely easy to rot and deteriorate, emits foul smell, and spreads bacteria and viruses, the main components of the kitchen waste comprise rice and flour food residues, vegetables, animal and vegetable oil, meat and bones and the like, and the kitchen waste comprises starch, cellulose, protein, lipid, inorganic salt and other components in terms of chemical composition. With the rapid development of economy in China, the yield of kitchen waste is larger and larger, and the research on the recycling of the kitchen waste is more and more; the method for producing the fuel ethanol and the lactic acid by utilizing the kitchen waste is an effective way for realizing high-value utilization of the fuel ethanol and the lactic acid.
The chinese patent with application number 201310437655.0 discloses a method for promoting the production of ethanol and lactic acid from kitchen waste by hydrothermal treatment, namely, the kitchen waste is subjected to hydrothermal pretreatment before fermentation, the yield of the ethanol obtained by the method is low, and the utilization rate of the kitchen waste is low. Therefore, a treatment method with high lactic acid yield and high utilization rate of the kitchen waste still needs to be researched.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide a method for efficiently producing lactic acid by utilizing kitchen waste.
The purpose of the invention is realized by the following technical scheme:
a method for efficiently producing lactic acid by utilizing kitchen waste comprises the following steps:
s1, pretreatment: carrying out hydrothermal pretreatment on the kitchen waste, and then separating solid kitchen waste;
s2 lactic acid fermentation: adding lactic acid fermentation bacteria into the solid kitchen waste separated in the S1, and separating after fermentation to obtain lactic acid;
wherein the lactic acid fermentation bacteria in S2 are added in twice, and the adding sequence is as follows: adding at least lactobacillus (Lactobacillus) The lactic acid fermenting bacteria 1, and at least one rumen coccus (A), (B) and (C)Ruminococcus) Amino acid bacterium (A), (B), (C)Aminobacterium) Aspergillus (a), (b)Aspergillus) And yeasts (Saccharomyces) The lactic acid fermenting bacteria group 2.
In the lactic acid fermentation process of the present invention, the inventors found that if lactic acid fermenting bacteria are added to solid kitchen waste in batches, the lactic acid obtained by fermentation has a high yield. Especially when lactobacillus (A), (B) is addedLactobacillus) The lactic acid fermenting bacteria 1 is fermented for a plurality of times, and then rumen coccus (A), (B) is addedRuminococcus) Amino acid bacterium (A), (B), (C)Aminobacterium) Aspergillus (a), (b)Aspergillus) And yeasts (Saccharomyces) The lactic acid fermenting bacteria group 2; the yield of lactic acid obtained by fermentation using this order of addition is highest.
Preferably, the lactic acid fermenting bacteria of S2 further comprise at least Streptococcus (S) ((S))Streptococcus) Bifidobacterium (b), (c), (d), (Bifidobacterium) Bacillus bacteria (b), (b)Bacillus) Lactococcus (A) and (B)Lactococcus) Clostridium (I), (II)Clostridiisalibacter）、A55-D21Spirochete (Spirochaeta), saccule bacterium (A), (B), (CAscosphaera) Rhizopus and rhizopus (Rhizopus) Vibrio acetobacter (Acetivibrio) Alcaligenes, Alcaligenes (II) and (III)Alcaligenes) Bacteroides (A), (B)Bacteroides) Violomonas bacterium (I), (II)Porphyromonadaceae) Or Pseudomonas bacteria (Pseudomonas) One or more of them.
The invention discloses a kitchen waste pretreatment method, which mainly aims to hydrolyze macromolecular substances such as cellulose, starch, protein and the like in the kitchen waste into small molecules so as to facilitate the production of lactic acid by subsequent microbial fermentation. The hydrothermal treatment is to perform thermochemical treatment on the kitchen waste in the presence of water, so that the diffusion coefficient of solid particles can be increased, organic matters are further decomposed through heat conduction, and the reaction time is shortened.
Preferably, before S1, the kitchen waste is sorted to remove impurities which cannot be fermented in the kitchen waste.
Preferably, in S1, the pretreatment includes sequentially subjecting the sorted kitchen waste to ultrasonic treatment, hydrothermal treatment and microwave treatment, and then separating solid kitchen waste. Firstly, carrying out ultrasonic treatment on the kitchen waste, and enabling solid particles to be violently impacted under the mechanical action of the kitchen waste, so that the formed cavitation action promotes the dispersion of the solid; in addition, when the ultrasonic waves are absorbed by the solid particles, a large amount of heat is generated, so that the temperature of the kitchen waste is suddenly increased, C-C bonds are broken, macromolecular hydrocarbon is decomposed into micromolecular hydrocarbon, and the hydrolysis of the kitchen waste is promoted. Microwave treatment can lead the small molecular substances to be directionally catalyzed and cracked, and promote hydrolysis reaction. After the kitchen waste is subjected to ultrasonic wave, hydrothermal treatment and microwave pretreatment in sequence, the hydrolysis effect of the kitchen waste can be obviously improved, and the yield of a subsequent fermentation product of the kitchen waste is effectively improved.
Preferably, the ultrasonic treatment conditions are as follows: the ultrasonic power is 15-1500 w/L, and the processing time is 5-60 min.
Preferably, the hydrothermal treatment conditions are: the hydrothermal temperature is 90-300 ℃, and the treatment time is 5-150 min.
Preferably, the microwave treatment conditions are as follows: the microwave power is 100-2500 kw, the time is 2-120 min, and the temperature is 90-300 ℃.
The ultrasonic treatment, the hydrothermal treatment and the microwave treatment can be sequentially carried out in an integrated device provided with an ultrasonic generator, a hydrothermal device and a microwave generator, and also can be sequentially carried out in an ultrasonic reactor, a hydrothermal device and a microwave reactor respectively.
Preferably, ethanol fermentation bacteria are added into the solid kitchen waste separated in S1, ethanol is separated after fermentation, and the obtained fermentation residue is used for fermenting lactic acid. More preferably, the ethanol fermentation bacteria at least comprise Bacillus (B)Bacillus) Lactobacillus, Lactobacillus (II)Lactobacillus) Leuconostoc (Leuconostoc sp.)Leuconostoc) Lactococcus (A) and (B)Lactococcus) Clostridium (I), (II)Clostridiisalibacter) Rumen coccus (A)Ruminococcus）、A55-D21Comamonas (a)Comamonas) Spirobacteria (A)Spira) Klebsiella bacterium (C.), (Klebsiella) Amino acid bacterium (A), (B), (C)Aminobacterium) Saccule bacterium (A), (B), (C)Ascosphaera) Aspergillus (a), (b)Aspergillus) Rhizopus and rhizopus (Rhizopus) Yeast, (b) yeastSaccharomyces) Vibrio acetobacter (Acetivibrio) Alcaligenes, Alcaligenes (II) and (III)Alcaligenes) Bacteroides (A), (B)Bacteroides) Violomonas bacterium (I), (II)Porphyromonadaceae) Or Pseudomonas bacteria (Pseudomonas) One or more of (a).
Preferably, the lactic acid fermenting bacteria of S2 is added in an amount of 0.01-20% (by volume) for 1-30 days.
Preferably, S2 is further added with lactic acid fermentation enzyme, the addition amount of the lactic acid fermentation enzyme is 0.01-15% (volume ratio), and the enzymolysis time is 1-144 hours.
Preferably, the enzyme for lactic acid fermentation is selected from one or more of cellulase, hemicellulase, ligninase, lipase, amylase, protease, β -glucosidase and lactate dehydrogenase.
Preferably, the addition amount of the ethanol fermentation bacteria is 0.01-20% (volume ratio), and the fermentation time is 1-25 days.
Preferably, the ethanol fermentation stage is also added with an enzyme for ethanol fermentation, the total addition amount of the enzyme for ethanol fermentation is 0.01-15% (volume ratio), and the enzymolysis time is 1-120 hours.
Preferably, the enzyme for ethanol fermentation is selected from one or more of ethanol dehydrogenase, acetaldehyde dehydrogenase, pyruvate carboxylase, amylase, cellulase, hemicellulase, ligninase, lipase, protease, saccharifying enzyme, pectinase, xylanase or β -glucanase.
In the invention, acid or/and alkali pretreatment is carried out before lactic acid fermentation, so that the hydrolysis rate of the kitchen waste can be increased, and the yield of the product ethanol is further improved. Therefore, the kitchen waste is preferably subjected to acid or/and alkali pretreatment before the ethanol fermentation.
In the invention, S1, the solid kitchen waste is separated through a three-phase separation operation, the three-phase separation is to discharge the kitchen waste in a spray explosion mode, then to stand to remove the upper layer of grease to obtain the solid kitchen waste, and the kitchen waste after pretreatment is discharged in the spray explosion mode to ensure that the grease in the kitchen waste can be separated from the solid and water to the maximum extent, so as to increase the precipitation amount of the floatable oil.
Preferably, an electric field is applied to carry out electric treatment in the ethanol fermentation and/or lactic acid fermentation processes. More preferably, the voltage of the electric field is 0.1-12V, and the processing time is 6-216 h. The electric field is used for acting on the microbial fermentation, so that the metabolism of the microbes can be stimulated, and the yield of the target product ethanol and/or lactic acid is improved.
In the invention, the temperature in the ethanol fermentation process is 20-80 ℃, and the temperature in the lactic acid fermentation process is 18-80 ℃.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a method for efficiently producing lactic acid from kitchen waste, which comprises the steps of performing hydrothermal pretreatment on sorted kitchen waste, and then adding microorganisms into solid kitchen waste for lactic acid fermentation. Meanwhile, the invention also provides a three-step pretreatment mode, namely, ultrasonic waves, hydrothermal treatment and microwave treatment are adopted to carry out pretreatment on the kitchen waste in sequence, so that the kitchen waste is hydrolyzed more thoroughly, and efficient microorganisms are subsequently adopted to carry out lactic acid fermentation; greatly improves the yield of the product lactic acid, has short fermentation time, has great popularization and application value, and is very suitable for industrialized application.
Detailed Description
The following examples are presented to further illustrate the present invention and should not be construed as limiting the invention. It is within the scope of the present invention to make simple modifications or alterations to the methods, procedures or conditions of the present invention without departing from the spirit and substance of the invention; unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art.
Example 1
A method for treating kitchen waste to efficiently produce lactic acid comprises the following steps:
s1, sorting: removing impurities such as chopsticks and plastics which cannot be used for fermentation in the kitchen waste;
s2, pretreatment: carrying out hydrothermal pretreatment on the sorted kitchen waste; the temperature of the hydrothermal treatment is 180 ℃, and the treatment time is 80 min;
s3, three-phase separation: discharging the kitchen waste in a spray explosion mode, standing, and removing upper-layer grease to obtain solid kitchen waste;
s4 lactic acid fermentation: adding lactic acid fermenting bacteria group 1[ Lactobacillus: (A)Lactobacillus）]Fermenting at 35 deg.C for 12 hr, and adding lactic acid fermenting bacteria 2[ Ruminococcus: (A)Ruminococcus) Amino acid bacterium (A), (B), (C)Aminobacterium) Aspergillus (Aspergillus) and yeast (Saccharomyces)]Fermenting for 216h, and extracting lactic acid from the fermentation broth.
In this example, the amount of the lactic acid fermenting bacteria added was 8% (by volume).
Example 2
A method for treating kitchen waste to efficiently produce lactic acid comprises the following steps:
s1, sorting: removing impurities such as chopsticks and plastics which cannot be used for fermentation in the kitchen waste;
s2, pretreatment: carrying out hydrothermal pretreatment on the sorted kitchen waste; the temperature of the hydrothermal treatment is 180 ℃, and the treatment time is 80 min;
s3, three-phase separation: discharging the kitchen waste in a spray explosion mode, standing, and removing upper-layer grease to obtain solid kitchen waste;
s4, ethanol fermentation: adding bacteria [ bacillus (Bacillus) for ethanol fermentation into the solid kitchen waste separated from the S3Bacillus) Lactobacillus, Lactobacillus (II)Lactobacillus) Leuconostoc (Leuconostoc sp.)Leuconostoc) Rhizopus (Rhizopus) and yeast (Saccharomyces)]Fermenting at 35 deg.C for 228 h; adding cellulase for enzymolysis at 35 deg.C for 12 hr, and extracting ethanol from the fermentation broth;
s5 lactic acid fermentation: adding lipase into the ethanol fermentation residue, performing enzymolysis at 35 deg.C for 12 hr, and adding lactic acid fermentation bacteria group 1[ Lactobacillus (L.), (Lactobacillus) And streptococcus (Streptococcus）]Fermenting at 35 deg.C for 12 hr, and adding lactic acid fermenting bacteria 2[ Ruminococcus: (A)Ruminococcus) Amino acid bacterium (A), (B), (C)Aminobacterium) Aspergillus (Aspergillus) and yeast (Saccharomyces)]Fermenting for 216h, and extracting lactic acid from the fermentation broth.
In this example, the amount of the ethanol fermentation bacteria was 5% (by volume), and the amount of the ethanol fermentation enzyme was 2% (by volume). The addition amount of the lactic acid fermentation bacteria is 8% (volume ratio), and the addition amount of the lactic acid fermentation enzyme is 1% (volume ratio).
Example 3
A method for treating kitchen waste to efficiently produce lactic acid comprises the following steps:
s1, sorting: removing impurities such as chopsticks and plastics which cannot be used for fermentation in the kitchen waste; sequentially adding sodium hydroxide and hydrochloric acid into the kitchen waste to ensure that the volume concentration of the sodium hydroxide and the volume concentration of the hydrochloric acid in the kitchen waste are respectively 0.5% and 1%;
s2, three-step pretreatment: sequentially carrying out ultrasonic treatment, hydrothermal treatment and microwave treatment on the kitchen waste treated by the S1; wherein the power of the ultrasonic wave is 900W/L, and the processing time is 20 min; the temperature of the hydrothermal treatment is 170 ℃, and the treatment time is 80 min; the power of microwave treatment is 900 kw, the temperature is maintained at 100 ℃, and the treatment time is 15 min; the three steps of pretreatment are respectively carried out in an ultrasonic reactor, a hydrothermal device and a microwave reactor in sequence;
s3, three-phase separation: discharging the kitchen waste in a spray explosion mode, standing, and removing upper-layer grease to obtain solid kitchen waste;
s4, ethanol fermentation: adding a mixture of cellulase and amylase into the solid kitchen waste obtained in the step S3 for enzymolysis for 2 hours, and then adding a bacterium [ Comamonas comamonas ] (Comamonas) Spirobacteria (A)Spira) Klebsiella bacterium (C.), (Klebsiella) Amino acid bacterium (A), (B), (C)Aminobacterium) Bacteroides (A), (B)Bacteroides) Aspergillus (a), (b)Aspergillus) And rhizopus (Rhizopus）]Fermenting at 35 deg.C for 190 hr, and extracting ethanol from the fermentation broth;
s5 lactic acid fermentation: adding lactic acid fermentation bacteria group 1[ Lactobacillus (L.), (L.) to the ethanol fermentation residueLactobacillus) And streptococcus (Streptococcus）]Fermenting at 35 deg.C for 12 hr, and adding lactic acid fermenting bacteria 2[ Ruminococcus: (A)Ruminococcus) Amino acid bacterium (A), (B), (C)Aminobacterium) Aspergillus (Aspergillus) and yeast (Saccharomyces)]Fermenting for 384h, and finally adding amylase and cellulase for enzymolysis for 168 h at 30 ℃; and extracting lactic acid from the fermentation liquor after the enzymolysis is finished.
In this example, the amount of the ethanol fermentation bacteria added was 0.01% (by volume), and the amount of the ethanol fermentation enzyme added was 0.1% (by volume). The addition amount of the lactic acid fermentation bacteria was 0.01% (volume ratio), and the addition amount of the lactic acid fermentation enzyme was 0.1% (volume ratio).
Example 4
The method provided in this example is the same as example 2, except that the electric field is switched on for electrical treatment in the lactic acid fermentation stage of S5, the voltage of the electric field is 0.1V, and the treatment time is 216 h. And (3) in the lactic acid fermentation stage of S5, switching in an electric field for electric treatment, wherein the voltage of the electric field is 12V, and the treatment time is 72 h.
In this example, the amount of the ethanol fermentation bacteria added was 20%, and the amount of the ethanol fermentation enzyme added was 0.1%. The addition amount of the lactic acid fermentation bacteria is 20%, and the addition amount of the lactic acid fermentation enzyme is 15%.
Example 5
The method provided by this embodiment is the same as embodiment 2, except that the pretreatment in S2 of this embodiment is performed by the following steps: sequentially carrying out ultrasonic treatment, hydrothermal treatment and microwave treatment on the kitchen waste treated by the S1; wherein the power of the ultrasonic wave is 15W/L, and the processing time is 5 min; the temperature of the hydrothermal treatment is 90 ℃, and the treatment time is 5 min; the microwave treatment power is 100kw, the temperature is maintained at 90 deg.C, and the treatment time is 2 min.
In this example, the amount of the ethanol fermentation bacteria was 5% (by volume), and the amount of the ethanol fermentation enzyme was 2% (by volume); the addition amount of the lactic acid fermentation bacteria is 8% (volume ratio), and the addition amount of the lactic acid fermentation enzyme is 1% (volume ratio).
Example 6
The method provided by this embodiment is the same as embodiment 2, except that the pretreatment in S2 of this embodiment is performed by the following steps: sequentially carrying out ultrasonic treatment, hydrothermal treatment and microwave treatment on the kitchen waste treated by the S1; wherein the power of the ultrasonic wave is 1500W/L, and the processing time is 60 min; the temperature of the hydrothermal treatment is 250 ℃, and the treatment time is 150 min; the microwave treatment power is 2500 kw, the temperature is maintained at 250 deg.C, and the treatment time is 120 min.
In this example, the amount of the ethanol fermentation bacteria added was 20% (by volume), and the amount of the ethanol fermentation enzyme added was 0.1%. The addition amount of the lactic acid fermentation bacteria is 20%, and the addition amount of the lactic acid fermentation enzyme is 15%.
Example 7
The method provided in this example is the same as example 2, except that no lactic acid fermentation enzyme was added during the lactic acid fermentation in S5. The lactic acid fermentation bacteria group 1 further contains Alcaligenes (II)Alcaligenes) Bacteroides (A), (B)Bacteroides) Violomonas, viologenBacteria (A), (B)Porphyromonadaceae) And Pseudomonas bacteria (Pseudomonas) (ii) a The lactic acid fermentation bacteria 2 further contains Bacillus bifidus (Bifidobacterium: (Bifidobacterium))Bifidobacterium) Bacillus bacteria (b), (b)Bacillus) Lactococcus (A) and (B)Lactococcus) Clostridium (I), (II)Clostridiisalibacter）、A55-D21Spirochete bacterium (a)Spirochaeta) And saccule bacterium (A), (B), (C)Ascosphaera）。
The total amount of each of the above-mentioned bacterial colonies and the fermentation time were the same as in example 2.
Comparative example 1
The method provided by the comparison example is the same as that of example 2, except that lactic acid fermentation bacteria are added after lipase is added into the ethanol fermentation residue for enzymolysis for 12 hours at 35 ℃, and the lactic acid fermentation bacteria selected in the lactic acid fermentation process in the comparison example S5 are Weissella, Lactobacillus plantarum and Rhizopus oryzae; the lactic acid fermentation bacteria were added at one time, and the total amount of the lactic acid fermentation bacteria and the total fermentation time were the same as in example 2.
Comparative example 2
The method of this comparative example is the same as that of example 2, except that in the lactic acid fermentation process of S5 in this comparative example, the lactic acid fermenting bacteria group 1 and the lactic acid fermenting bacteria group 2 were added simultaneously after the addition of the lactic acid fermentation enzyme, the total amount of the lactic acid fermenting bacteria was the same as that of example 2, and the fermentation time was 240 hours.
TABLE 1 concentrations of ethanol and lactic acid fermented by the methods provided in examples 1-5 and comparative examples 1-2
As can be seen from Table 1, in the method provided by the invention, the lactic acid fermentation bacteria are added into the solid kitchen waste in several times in the lactic acid fermentation process, so that the yield of the lactic acid obtained by fermentation is obviously improved.
Claims (10)
1. A method for efficiently producing lactic acid by utilizing kitchen waste is characterized by comprising the following steps:
s1, pretreatment: carrying out hydrothermal pretreatment on the kitchen waste, and then separating solid kitchen waste;
s2, lactic acid fermentation: adding lactic acid fermentation bacteria into the solid kitchen waste separated in the S1, and separating after fermentation to obtain lactic acid;
wherein the lactic acid fermentation bacteria in S2 are added in twice, and the adding sequence is as follows: the lactic acid fermenting bacteria group 1 including at least Lactobacillus (Lactobacillus) is added first, and then the lactic acid fermenting bacteria group 2 including at least Ruminococcus (Ruminococcus), aminobacillus (aminobacillus), Aspergillus (Aspergillus), and yeast (Saccharomyces) is added.
2. The method according to claim 1, wherein the lactic acid fermenting bacteria of S2 further comprise at least one or more of Streptococcus (Streptococcus), Bifidobacterium (Bifidobacterium), Bacillus (Bacillus), Lactococcus (Lactococcus), Clostridium (Clostridium), spirochaete (Spirochaeta), Gliococcus (Ascosphaera), Rhizopus (Rhizopus), Vibrio aceticus (Acetivibrio), Alcaligenes (Alcaligenes), Bacteroides (Bacteroides), Porphyromonas (Porphyromonaceae), and Pseudomonas (Pseudomonas).
3. The method according to claim 1, wherein before S1, the kitchen waste is sorted and impurities are removed; and S1, the pretreatment is to sequentially carry out ultrasonic, hydrothermal and microwave pretreatment on the sorted kitchen waste and then separate solid kitchen waste.
4. The method according to claim 1, wherein the lactic acid fermenting bacteria of S2 is added in an amount of 0.01 to 20% for a fermentation time of 1 to 30 days.
5. The method according to claim 1, wherein S2 further comprises lactic acid fermentation enzyme, wherein the addition amount of the lactic acid fermentation enzyme is 0.01-15%, and the enzymolysis time is 1-144 hours.
6. The method according to claim 1, characterized in that the kitchen waste is pre-treated with acid or/and alkali before being subjected to lactic acid fermentation.
7. The method according to claim 1, wherein the solid kitchen waste separated in S1 is added with ethanol fermentation bacteria, and ethanol is separated to obtain fermentation residue for lactic acid fermentation.
8. The method according to claim 7, wherein the total addition amount of the ethanol fermentation bacteria is 0.01-25%, and the fermentation time is 1-25 days; and in the ethanol fermentation stage, an enzyme for ethanol fermentation is also added, the total addition amount of the enzyme for ethanol fermentation is 0.01-15%, and the enzymolysis time is 1-120 hours.
9. The method according to any one of claims 1 to 8, wherein the hydrothermal treatment conditions of S1 are: the hydrothermal temperature is 90-300 ℃, and the treatment time is 5-150 min.
10. The method according to any one of claims 1 to 8, wherein an electric field is applied to perform the electrical treatment during the lactic acid fermentation process.
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| CN102424831A (en) * | 2011-12-16 | 2012-04-25 | 天津北洋百川生物技术有限公司 | Method for producing lactic acid by using food waste |
| CN103484521A (en) * | 2013-09-24 | 2014-01-01 | 华南农业大学 | Method adopting hydro-thermal treatment to facilitate producing ethyl alcohol and lactic acid through kitchen waste |
| CN103695525A (en) * | 2014-01-02 | 2014-04-02 | 华南农业大学 | Method for increasing lactic acid fermentation yield of kitchen waste employing hydro-thermal treatment |
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| CN102424831A (en) * | 2011-12-16 | 2012-04-25 | 天津北洋百川生物技术有限公司 | Method for producing lactic acid by using food waste |
| CN103484521A (en) * | 2013-09-24 | 2014-01-01 | 华南农业大学 | Method adopting hydro-thermal treatment to facilitate producing ethyl alcohol and lactic acid through kitchen waste |
| CN103695525A (en) * | 2014-01-02 | 2014-04-02 | 华南农业大学 | Method for increasing lactic acid fermentation yield of kitchen waste employing hydro-thermal treatment |
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