CN107119102A - A kind of method that utilization kitchen garbage efficiently produces lactic acid - Google Patents
A kind of method that utilization kitchen garbage efficiently produces lactic acid Download PDFInfo
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- CN107119102A CN107119102A CN201710415496.2A CN201710415496A CN107119102A CN 107119102 A CN107119102 A CN 107119102A CN 201710415496 A CN201710415496 A CN 201710415496A CN 107119102 A CN107119102 A CN 107119102A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/56—Lactic acid
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Abstract
The invention belongs to kitchen garbage processing technology field, a kind of method that utilization kitchen garbage efficiently produces lactic acid is specifically disclosed.It the described method comprises the following steps：S1. pre-process：Kitchen garbage is subjected to hydrothermal pretreatment, solid kitchen garbage is then demultiplex out；S2. lactic fermentation：Lactic fermentation is added in the solid kitchen garbage isolated into S1 with bacterium, isolated lactic acid after fermentation.Products collection efficiency greatly improved in the method that the present invention is provided, and fermentation time is short, with actual application value, is especially suitable for commercial application.
Description
Technical field
The present invention relates to kitchen garbage processing technology field, lactic acid is efficiently produced more particularly, to using kitchen garbage
Method.
Background technology
Kitchen garbage, is commonly called as hogwash, also known as swill, swill, is the sanitary waste that resident is formed during personal consumption,
Easily rot, distribute stench, spread germs and virus, kitchen garbage main component include rice and flours food residues,
Vegetables, vegetable and animals oils, meat bone etc., chemically say there are the compositions such as starch, cellulose, protein, lipid and inorganic salts on composition.
With the fast development of China's economy, the yield of kitchen garbage is increasing, and the research to the recycling of kitchen garbage is also more next
It is more；Wherein, it is the effective way of realizing its higher value application to produce alcohol fuel and lactic acid using kitchen garbage.
The Chinese patent of Application No. 201310437655.0 discloses a kind of hydro-thermal process and promotes kitchen garbage production second
The method of alcohol and lactic acid, i.e., carry out hydrothermal pretreatment before fermentation to kitchen garbage, and alcohol yied obtained by this method is relatively low, kitchen
Garbage utilization rate is relatively low.Therefore, still need to study the processing method that a kind of lactic acid yield is high, kitchen garbage utilization rate is high.
The content of the invention
The technical problems to be solved by the invention be overcome prior art exist drawbacks described above there is provided utilize kitchen garbage
The method for efficiently producing lactic acid, the yield and kitchen garbage that the method that the present invention is provided significantly improves target product lactic acid is utilized
Rate.
The purpose of the present invention is achieved by the following technical programs：
A kind of method that utilization kitchen garbage efficiently produces lactic acid, the described method comprises the following steps：
S1. pre-process：Kitchen garbage is subjected to hydrothermal pretreatment, solid kitchen garbage is then demultiplex out；
S2. lactic fermentation：Lactic fermentation is added in the solid kitchen garbage isolated to S1 with bacterium, isolated breast after fermentation
Acid；
Wherein, lactic fermentation described in S2 is added in two times with bacterium, is added order and is：First adding at least includes lactobacillus
（Lactobacillus）Lactic fermentation flora 1, then add at least include Ruminococcus（Ruminococcus）, amino
Bacillus（Aminobacterium）, Aspergillus（Aspergillus）And saccharomycete（Saccharomyces）Lactic fermentation flora
2。
In the lactic fermentation process of the present invention, inventor has found, if lactic fermentation is added portionwise into solid kitchen with bacterium
In rubbish, the lactic acid obtained by fermentation has higher yield.Especially add in the ban and include lactobacillus（Lactobacillus）
Lactic fermentation flora 1 fermentation some time after, add and include Ruminococcus（Ruminococcus）, amino bacillus
（Aminobacterium）, Aspergillus（Aspergillus）And saccharomycete（Saccharomyces）Lactic fermentation flora 2；Adopt
With the yield highest of the lactic acid obtained by the fermentation of such a order of addition.
Preferably, lactic fermentation described in S2 also at least contains streptococcus with bacterium（Streptococcus）, Bifidobacterium
（Bifidobacterium）, bacillus（Bacillus）, galactococcus（Lactococcus）, clostridium
（Clostridiisalibacter）、A55-D21, burgdorferi strain（Spirochaeta）, blastocyst（Ascosphaera）, head mold
Bacterium（Rhizopus）, vinegar vibrios（Acetivibrio）, Bacillus alcaligenes（Alcaligenes）, bacteroid（Bacteroides）, it is purple
Monad（Porphyromonadaceae）Or pseudomonad（Pseudomonas）In one or more.
The main purpose of kitchen garbage pretreatment is to make the macromolecular complex such as cellulose, starch, protein in kitchen garbage
Matter hydrolysis turns into small molecule, facilitates follow-up microbial fermentation production lactic acid, the present invention is in order to obtain the lactic acid of high yield, to kitchen
Rubbish carries out hydrothermal pretreatment.Hydro-thermal process is that kitchen garbage is carried out into hot chemical treatment in the environment of having water, can make solid
Particle increases diffusion coefficient, by heat transfer, organic matter is further decomposed, and shortens the reaction time.
Preferably, sorting kitchen waste is carried out before S1, unfermentable debris in kitchen garbage is removed.
Preferably, pretreatment described in S1 is to carry out the kitchen garbage after sorting at ultrasonic wave, hydro-thermal and microwave successively
Reason, is then demultiplex out solid kitchen garbage.Kitchen garbage is passed through into ultrasonication first, the mechanism of generation makes solid
Intergranular hard hit, the cavitation of formation facilitates the scattered of solid；In addition, being produced when ultrasonic wave is absorbed by solid particle a large amount of
Heat, makes the temperature of kitchen garbage raise suddenly, makes C-C keys be broken, and the hydrocarbon of macromolecular is decomposed into the carbon of small molecule
Hydrogen compound, promotes the hydrolysis of kitchen garbage.Microwave treatment can crack small-molecule substance directional catalyzing, promote hydrolysis.
The hydrolysis effect of kitchen garbage can be significantly improved after ultrasonic wave, hydro-thermal, Microwave Pretreatment is carried out successively to kitchen garbage, and
Effectively improve the yield of its subsequent fermentation product.
Preferably, the treatment conditions of the ultrasonic wave are：15~1500w/L of ultrasonic power, processing time is 5~60
min。
Preferably, the treatment conditions of the hydro-thermal are：Hydrothermal temperature is 90~300 DEG C, and processing time is 5~150 min.
Preferably, the treatment conditions of the microwave are：The power of microwave is the kw of 100w~2500, the time is 2~
120min, temperature is 90~300 DEG C.
Ultrasonication of the present invention, hydro-thermal process and microwave treatment can be equipped with supersonic generator, hydro-thermal dresses
Put in the integration apparatus with microwave generator and carry out successively, can also be respectively in ultrasound reactor, hydrothermal device and microwave
Carried out successively in reactor.
Preferably, alcohol fermentation is added in the solid kitchen garbage isolated to S1 with bacterium, ethanol, institute are isolated after fermentation
Obtaining fermentation residue is used for fermentation lactic acid.It is further preferable that the alcohol fermentation comprises at least bacillus with bacterium（Bacillus）、
Lactobacillus（Lactobacillus）, leukonid（Leuconostoc）, galactococcus（Lactococcus）, clostridium
（Clostridiisalibacter）, Ruminococcus（Ruminococcus）、A55-D21, comamonas（Comamonas）、
Spirillum（Spira）, klebsiella（Klebsiella）, amino bacillus（Aminobacterium）, blastocyst
（Ascosphaera）, Aspergillus（Aspergillus）, rhizopus（Rhizopus）, saccharomycete（Saccharomyces）, vinegar arc
Bacterium（Acetivibrio）, Bacillus alcaligenes（Alcaligenes）, bacteroid（Bacteroides）, purple monad
（Porphyromonadaceae）Or pseudomonad（Pseudomonas）In one or more.
Preferably, lactic fermentation described in S2 is 0.01~20% with the addition of bacterium（Volume ratio）, fermentation time is 1~30
My god.
Preferably, S2 is also added with lactic fermentation enzyme, and the addition of the lactic fermentation enzyme is 0.01%~15%（Body
Product ratio）, enzymolysis time is 1~144 hour.
Preferably, the lactic fermentation is selected from cellulase, hemicellulase, lignoenzyme, lipase, starch with enzyme
One or more in enzyme, protease, beta-glucosidase, lactic dehydrogenase.
Preferably, the alcohol fermentation is 0.01~20% with the addition of bacterium（Volume ratio）, fermentation time is 1~25 day.
Preferably, alcohol fermentation enzyme, the total addition level of the alcohol fermentation enzyme are also added in the alcohol fermentation stage
For 0.01~15%（Volume ratio）, enzymolysis time is 1~120 hour.
Preferably, the alcohol fermentation with enzyme be selected from alcohol dehydrogenase, acetaldehyde dehydrogenase, pyruvate carboxylase, amylase,
Cellulase, hemicellulase, lignoenzyme, lipase, protease, carbohydrase, pectase, zytase or 1,4 beta-glucanase
In one or more.
In the present invention, acid or/and oxygenation pretreatment are carried out before lactic fermentation, kitchen garbage hydrolysis rate can be increased, entered
One step improves the yield of product ethanol.It is preferred that kitchen garbage first carries out acid or/and oxygenation pretreatment before alcohol fermentation.
In the present invention, the separation of solid kitchen garbage described in S1 is operated by three phase separation and completed, the three phase separation
It is, to spray after quick-fried mode discharges, to stand kitchen garbage and remove upper strata grease, obtain solid kitchen garbage, spray quick-fried mode and arrange
Going out pretreated kitchen garbage allows the grease in kitchen garbage farthest to be separated with solid, water, and increase can be floated
Oily amount of precipitation.
Preferably, electric field progress electric treatment is accessed in alcohol fermentation and/or lactic fermentation process.It is further preferable that described
The voltage of electric field is 0.1~12V, and processing time is 6~216h.Using electric field action in microbial fermentation, microorganism can be stimulated
Metabolism, so as to improve the yield of purpose product ethanol and/or lactic acid.
In the present invention, the temperature in alcohol fermentation processes is 20~80 DEG C, temperature in lactic fermentation process for 18~
80℃。
Compared with prior art, the invention has the advantages that：
The invention provides a kind of method that kitchen garbage efficiently produces lactic acid, the Kitchen waste hydrothermal after will sorting is located in advance
Reason, then adds microorganism into solid kitchen garbage and carries out lactic fermentation.The invention also provides three step pretreatment sides simultaneously
Formula, i.e., pre-processed to kitchen garbage, make its hydrolysis more thorough successively using ultrasonic wave, hydro-thermal, microwave, follow-up using high
Imitate microorganism and carry out lactic fermentation；The yield of product lactic acid greatly improved, fermentation time is short, with great popularization and application valency
Value, is especially suitable for commercial application.
Embodiment
Present disclosure is further illustrated with reference to specific embodiment, but be should not be construed as limiting the invention.
Without departing from the spirit and substance of the case in the present invention, the simple modifications or substitutions made to the inventive method, step or condition,
Belong to the scope of the present invention；Unless otherwise specified, technological means used in embodiment is well known to those skilled in the art
Conventional meanses.
Embodiment 1
It is a kind of to handle the method that kitchen garbage efficiently produces lactic acid, comprise the following steps：
S1. sort：Remove the debris that chopsticks, plastics in kitchen garbage etc. cannot be used for fermentation；
S2. pre-process：Kitchen garbage after sorting is subjected to hydrothermal pretreatment；The temperature of hydro-thermal process is 180 DEG C, during processing
Between be 80 min；
S3. three phase separation：Kitchen garbage is stood with spraying after quick-fried mode is discharged, upper strata grease is removed, obtains solid kitchen rubbish
Rubbish；
S4. lactic fermentation：Add [the lactobacillus of lactic fermentation flora 1（Lactobacillus）] ferment 12h under the conditions of 35 DEG C,
Then [the Ruminococcus of lactic fermentation flora 2 is added（Ruminococcus）, amino bacillus（Aminobacterium）, aspergillus
Bacterium（Aspergillus）And saccharomycete（Saccharomyces）] fermentation 216h, lactic acid is finally extracted from zymotic fluid.
In the present embodiment, the lactic fermentation is 8% with the addition of bacterium（Volume ratio）.
Embodiment 2
It is a kind of to handle the method that kitchen garbage efficiently produces lactic acid, comprise the following steps：
S1. sort：Remove the debris that chopsticks, plastics in kitchen garbage etc. cannot be used for fermentation；
S2. pre-process：Kitchen garbage after sorting is subjected to hydrothermal pretreatment；The temperature of hydro-thermal process is 180 DEG C, during processing
Between be 80 min；
S3. three phase separation：Kitchen garbage is stood with spraying after quick-fried mode is discharged, upper strata grease is removed, obtains solid kitchen rubbish
Rubbish；
S4. alcohol fermentation：Alcohol fermentation is added in the solid kitchen garbage isolated to S3 with bacterium [bacillus
（Bacillus）, lactobacillus（Lactobacillus）, leukonid（Leuconostoc）, rhizopus（Rhizopus）And yeast
Bacterium（Saccharomyces）] ferment 228h under the conditions of 35 DEG C；Then cellulase is added in being digested at 35 DEG C after 12h, from hair
Ethanol is extracted in zymotic fluid；
S5. lactic fermentation：Added into ethanol fermentation residue at 35 DEG C of lipase and digest 12h, then add lactic fermentation flora
1 [lactobacillus（Lactobacillus）And streptococcus（Streptococcus）] ferment 12h under the conditions of 35 DEG C, then add
[the Ruminococcus of lactic fermentation flora 2（Ruminococcus）, amino bacillus（Aminobacterium）, Aspergillus
（Aspergillus）And saccharomycete（Saccharomyces）] fermentation 216h, lactic acid is finally extracted from zymotic fluid.
In the present embodiment, the alcohol fermentation is 5% with the addition of bacterium（Volume ratio）, the alcohol fermentation enzyme
Addition is 2%（Volume ratio）.The lactic fermentation is 8% with the addition of bacterium（Volume ratio）, the lactic fermentation enzyme adds
Dosage is 1%（Volume ratio）.
Embodiment 3
It is a kind of to handle the method that kitchen garbage efficiently produces lactic acid, comprise the following steps：
S1. sort：Remove the debris that chopsticks, plastics in kitchen garbage etc. cannot be used for fermentation；Add successively in kitchen garbage
Enter sodium hydroxide and hydrochloric acid so that the volumetric concentration of sodium hydroxide and hydrochloric acid in kitchen garbage is respectively 0.5% and 1%；
S2. three steps are pre-processed：Kitchen garbage after S1 is handled carries out ultrasonic wave, hydro-thermal and microwave treatment successively；It is wherein ultrasonic
The power of ripple is 900 W/L, the min of processing time 20；The temperature of hydro-thermal process is 170 DEG C, and processing time is 80 min；Microwave
The power of processing is 900 kw, and temperature maintains 100 DEG C, and processing time is 15 min；The three steps pretreatment is respectively in ultrasound
Carried out successively in ripple reactor, hydrothermal device and microwave reactor；
S3. three phase separation：Kitchen garbage is stood with spraying after quick-fried mode is discharged, upper strata grease is removed, obtains solid kitchen rubbish
Rubbish；
S4. alcohol fermentation：The mixture that cellulase and amylase are added in the solid kitchen garbage obtained to S3 digests 2 h,
It is subsequently added into alcohol fermentation bacterium [comamonas（Comamonas）, spirillum（Spira）, klebsiella
（Klebsiella）, amino bacillus（Aminobacterium）, bacteroid（Bacteroides）, Aspergillus（Aspergillus）
And rhizopus（Rhizopus）] in after fermentation 190h under the conditions of 35 DEG C, ethanol is extracted from zymotic fluid；
S5. lactic fermentation：[the lactobacillus of lactic fermentation flora 1 is added into ethanol fermentation residue（Lactobacillus）And chain
Coccus（Streptococcus）] ferment 12h under the conditions of 35 DEG C, then adds the [Ruminococcus of lactic fermentation flora 2
（Ruminococcus）, amino bacillus（Aminobacterium）, Aspergillus（Aspergillus）And saccharomycete
（Saccharomyces）] fermentation 384h, it is eventually adding at 30 DEG C of amylase and cellulase and digests 168 h；After enzymolysis is completed
Lactic acid is extracted from zymotic fluid.
In the present embodiment, the alcohol fermentation is 0.01% with the addition of bacterium（Volume ratio）, the alcohol fermentation enzyme
Addition be 0.1%（Volume ratio）.The lactic fermentation is 0.01% with the addition of bacterium（Volume ratio）, the lactic fermentation use
The addition of enzyme is 0.1%（Volume ratio）.
Embodiment 4
The method be the same as Example 2 that the present embodiment is provided, difference is, electric field is accessed in the S5 lactic fermentation stages and is carried out at electricity
Reason, the voltage of the electric field is 0.1V, and processing time is 216h.Electric field is accessed in the S5 lactic fermentation stages and carries out electric treatment, institute
Voltage of electric field is stated for 12V, processing time is 72h.
In the present embodiment, the alcohol fermentation is 20% with the addition of bacterium, and the alcohol fermentation is with the addition of enzyme
0.1%.The lactic fermentation is 20% with the addition of bacterium, and the addition of the lactic fermentation enzyme is 15%.
Embodiment 5
The method be the same as Example 2 that the present embodiment is provided, difference is, pre-processes to enter according to the following steps described in the present embodiment S2
OK：Kitchen garbage after S1 is handled carries out ultrasonic wave, hydro-thermal and microwave treatment successively；Wherein the power of ultrasonic wave is 15W/L,
The min of processing time 5；The temperature of hydro-thermal process is 90 DEG C, and processing time is 5 min；The power of microwave treatment is 100 kw, temperature
Degree maintains 90 DEG C, and processing time is 2 min.
In the present embodiment, the alcohol fermentation is 5% with the addition of bacterium（Volume ratio）, the alcohol fermentation enzyme
Addition is 2%（Volume ratio）；The lactic fermentation is 8% with the addition of bacterium（Volume ratio）, the lactic fermentation enzyme adds
Dosage is 1%（Volume ratio）.
Embodiment 6
The method be the same as Example 2 that the present embodiment is provided, difference is, pre-processes to enter according to the following steps described in the present embodiment S2
OK：Kitchen garbage after S1 is handled carries out ultrasonic wave, hydro-thermal and microwave treatment successively；Wherein the power of ultrasonic wave is 1500W/
L, the min of processing time 60；The temperature of hydro-thermal process is 250 DEG C, and processing time is 150 min；The power of microwave treatment is 2500
Kw, temperature maintains 250 DEG C, and processing time is 120 min.
In the present embodiment, the alcohol fermentation is 20% with the addition of bacterium（Volume ratio）, the alcohol fermentation enzyme
Addition is 0.1%.The lactic fermentation is 20% with the addition of bacterium, and the addition of the lactic fermentation enzyme is 15%.
Embodiment 7
The method be the same as Example 2 that the present embodiment is provided, difference is, is used in S5 lactic fermentation process without lactic fermentation
Enzyme.Also contain Bacillus alcaligenes in lactic fermentation flora 1（Alcaligenes）, bacteroid（Bacteroides）, purple monad
（Porphyromonadaceae）And pseudomonad（Pseudomonas）；Also contain Bifidobacterium in lactic fermentation flora 2
（Bifidobacterium）, bacillus（Bacillus）, galactococcus（Lactococcus）, clostridium
（Clostridiisalibacter）、A55-D21, burgdorferi strain（Spirochaeta）And blastocyst（Ascosphaera）.
The total consumption and fermentation time of above-mentioned each flora are same as Example 2.
Comparative example 1
The method be the same as Example 2 that this reference examples is provided, difference is, 35 DEG C of lipase is being added into ethanol fermentation residue
Lactic fermentation is added after lower enzymolysis 12h with bacterium, in this reference examples S5 in lactic fermentation process, selected lactic fermentation is with bacterium
Wei Si Salmonellas, Lactobacillus plantarum and Rhizopus oryzae；The lactic fermentation is once to add with bacterium, when its total amount of adding is with fermenting total
Between it is same as Example 2.
Comparative example 2
The method be the same as Example 2 that this reference examples is provided, difference is, in this reference examples in S5 lactic fermentation process, lactic acid
The addition simultaneously after addition lactic fermentation enzyme of fermentative microflora 1 and lactic fermentation flora 2, the lactic fermentation total consumption and reality of bacterium
Apply example 2 identical, fermentation time is 240h.
Ethanol and lactic acid concn that the method fermentation that the embodiment 1~5 of table 1 and comparative example 1~2 are provided is obtained
As shown in Table 1, lactic fermentation is added solid kitchen by the method that the present invention is provided by several times in lactic fermentation process with bacterium
In rubbish, the yield for the lactic acid that fermentation is obtained is significantly improved.
Claims (10)
1. a kind of method that utilization kitchen garbage efficiently produces lactic acid, it is characterised in that the described method comprises the following steps：
S1. pre-process：Kitchen garbage is subjected to hydrothermal pretreatment, solid kitchen garbage is then demultiplex out；
S2. lactic fermentation：Lactic fermentation is added in the solid kitchen garbage isolated to S1 with bacterium, isolated breast after fermentation
Acid；
Wherein, lactic fermentation described in S2 is added in two times with bacterium, is added order and is：First adding at least includes lactobacillus
（Lactobacillus）Lactic fermentation flora 1, then add at least include Ruminococcus（Ruminococcus）, amino bar
Bacterium（Aminobacterium）, Aspergillus（Aspergillus）And saccharomycete（Saccharomyces）Lactic fermentation flora 2.
2. method according to claim 1, it is characterised in that lactic fermentation also at least contains streptococcus with bacterium described in S2
（Streptococcus）, Bifidobacterium（Bifidobacterium）, bacillus（Bacillus）, galactococcus
（Lactococcus）, clostridium（Clostridiisalibacter）、A55-D21, burgdorferi strain（Spirochaeta）, blastocyst
（Ascosphaera）, rhizopus（Rhizopus）, vinegar vibrios（Acetivibrio）, Bacillus alcaligenes（Alcaligenes）, intend bar
Bacterium（Bacteroides）, purple monad（Porphyromonadaceae）, pseudomonad（Pseudomonas）In one kind or
It is several.
3. method according to claim 1, it is characterised in that kitchen garbage is sorted before S1, debris is removed；Locate in advance described in S1
Manage the kitchen garbage after sorting carrying out ultrasonic wave, hydro-thermal and Microwave Pretreatment successively, solid kitchen garbage is then demultiplex out.
4. method according to claim 1, it is characterised in that lactic fermentation described in S2 is 0.01~20% with the addition of bacterium,
Fermentation time is 1~30 day.
5. method according to claim 1, it is characterised in that lactic fermentation enzyme, the lactic fermentation are also added in S2
It is 0.01~15% with the addition of enzyme, enzymolysis time is 1~144 hour.
6. method according to claim 1, it is characterised in that kitchen garbage carried out in advance before lactic fermentation is carried out acid or/
And oxygenation pretreatment.
7. method according to claim 1, it is characterised in that add alcohol fermentation in the solid kitchen garbage isolated to S1
With bacterium, isolate ethanol and obtain fermentation residue for lactic fermentation.
8. method according to claim 7, it is characterised in that the alcohol fermentation is 0.01~25% with the total addition level of bacterium,
Fermentation time is 1~25 day；The alcohol fermentation stage also added alcohol fermentation enzyme, the total addition level of the alcohol fermentation enzyme
For 0.01~15%, enzymolysis time is 1~120 hour.
9. according to any methods described of claim 1 to 8, it is characterised in that the treatment conditions of hydro-thermal described in S1 are：Hydrothermal temperature
For 90~300 DEG C, processing time is 5~150 min.
10. according to any methods described of claim 1 to 8, it is characterised in that electric field is accessed in lactic fermentation process and is carried out at electricity
Reason.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108342419A (en) * | 2018-04-18 | 2018-07-31 | 东华大学 | A method of continuously fermented using debirs and prepares high-purity L-lactic acid |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102424831A (en) * | 2011-12-16 | 2012-04-25 | 天津北洋百川生物技术有限公司 | Method for producing lactic acid by using food waste |
| CN103484521A (en) * | 2013-09-24 | 2014-01-01 | 华南农业大学 | Method adopting hydro-thermal treatment to facilitate producing ethyl alcohol and lactic acid through kitchen waste |
| CN103695525A (en) * | 2014-01-02 | 2014-04-02 | 华南农业大学 | Method for increasing lactic acid fermentation yield of kitchen waste employing hydro-thermal treatment |
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- 2017-06-05 CN CN201710415496.2A patent/CN107119102B/en active Active
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102424831A (en) * | 2011-12-16 | 2012-04-25 | 天津北洋百川生物技术有限公司 | Method for producing lactic acid by using food waste |
| CN103484521A (en) * | 2013-09-24 | 2014-01-01 | 华南农业大学 | Method adopting hydro-thermal treatment to facilitate producing ethyl alcohol and lactic acid through kitchen waste |
| CN103695525A (en) * | 2014-01-02 | 2014-04-02 | 华南农业大学 | Method for increasing lactic acid fermentation yield of kitchen waste employing hydro-thermal treatment |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108342419A (en) * | 2018-04-18 | 2018-07-31 | 东华大学 | A method of continuously fermented using debirs and prepares high-purity L-lactic acid |
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