CN107115353A - A kind of coriolan of the methylselenocysteinefrom containing L and preparation method thereof - Google Patents
A kind of coriolan of the methylselenocysteinefrom containing L and preparation method thereof Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 63
- 239000000706 filtrate Substances 0.000 claims abstract description 38
- 238000000605 extraction Methods 0.000 claims abstract description 31
- 238000001556 precipitation Methods 0.000 claims abstract description 25
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims abstract description 24
- 229910052711 selenium Inorganic materials 0.000 claims abstract description 24
- 239000011669 selenium Substances 0.000 claims abstract description 24
- 238000001914 filtration Methods 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 238000010438 heat treatment Methods 0.000 claims abstract description 11
- 239000012535 impurity Substances 0.000 claims abstract description 6
- DXIMSLPBBITCAX-UHFFFAOYSA-N C[Se].[Se] Chemical group C[Se].[Se] DXIMSLPBBITCAX-UHFFFAOYSA-N 0.000 claims description 35
- 238000004821 distillation Methods 0.000 claims description 7
- 150000004676 glycans Chemical class 0.000 claims description 6
- 229920001282 polysaccharide Polymers 0.000 claims description 6
- 239000005017 polysaccharide Substances 0.000 claims description 6
- 238000005292 vacuum distillation Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 238000001291 vacuum drying Methods 0.000 claims description 5
- 230000000694 effects Effects 0.000 abstract description 6
- 230000007365 immunoregulation Effects 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 2
- 102000006587 Glutathione peroxidase Human genes 0.000 description 4
- 108700016172 Glutathione peroxidases Proteins 0.000 description 4
- 239000013049 sediment Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000000589 Interleukin-1 Human genes 0.000 description 2
- 108010002352 Interleukin-1 Proteins 0.000 description 2
- 102000000588 Interleukin-2 Human genes 0.000 description 2
- 108010002350 Interleukin-2 Proteins 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 230000003712 anti-aging effect Effects 0.000 description 2
- 230000003471 anti-radiation Effects 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 210000001185 bone marrow Anatomy 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- -1 lipid peroxide Chemical class 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 206010008909 Chronic Hepatitis Diseases 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108010024636 Glutathione Proteins 0.000 description 1
- 102000008070 Interferon-gamma Human genes 0.000 description 1
- 108010074328 Interferon-gamma Proteins 0.000 description 1
- 208000019926 Keshan disease Diseases 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 206010057249 Phagocytosis Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 235000020774 essential nutrients Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 229940044627 gamma-interferon Drugs 0.000 description 1
- 229960003180 glutathione Drugs 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000002955 immunomodulating agent Substances 0.000 description 1
- 230000002584 immunomodulator Effects 0.000 description 1
- 229940121354 immunomodulator Drugs 0.000 description 1
- 201000002364 leukopenia Diseases 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 230000000527 lymphocytic effect Effects 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 210000000865 mononuclear phagocyte system Anatomy 0.000 description 1
- 239000003176 neuroleptic agent Substances 0.000 description 1
- 230000000701 neuroleptic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 201000008482 osteoarthritis Diseases 0.000 description 1
- 230000000242 pagocytic effect Effects 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 230000008782 phagocytosis Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
- A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid or pantothenic acid
- A61K31/198—Alpha-amino acids, e.g. alanine or edetic acid [EDTA]
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Molecular Biology (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a kind of preparation method of the coriolan of methylselenocysteinefrom containing L, comprise the following steps：Step (1)：Rainbow conk is cleaned, rainbow conk is shredded；Step (2)：Rainbow conk in step (1) is mixed with water, extracting liq after heating, the removal of impurity is gone into the liquid filtering of extraction, filtrate is obtained；Step (3)：The ethanol of 3~4 times of filtrate volume is added in the filtrate obtained by step (2), 20~30 hours is stood, collects precipitation.Step (4)：Precipitation obtained by step (3) is washed 3~5 times with ethanol；Alcohol precipitation solution is washed off according to similar compatibility；Step (5)：Precipitation obtained by step (4) is dried, crushed, 80~150 mesh sieves is crossed, produces coriolan；Step (6)：Coriolan and L methylselenocysteinefroms obtained by step (5) is pressed 10000:1~1.5 weight is than well mixed.Technical scheme can will preferably produce Organism immunoregulation effect after coriolan and selenium compatibility.
Description
Technical field
The invention belongs to biological technical field, and in particular to a kind of coriolan of selenium-methyl selenium substituted aminothiopropionic containing L-
And preparation method thereof.
Background technology
Selenium is the essential trace elements of the human body, is the activated centre of glutathione peroxidase.Glutathione peroxidating
Thing enzyme is widely present in each histocyte and body fluid of animal and plant body, has protective effect to membrane structure.Selenium is used as paddy Guang
The necessary component of sweet peptide peroxidase (GSH-Px), the removing to interior free yl and lipid peroxide has played main work just,
It is the required composition for stablizing biomembrane.Free radical and its lipid peroxidation of induction are to biomembrane and DNA detrimental effect
Cause the major reason of biological decay.Selenium has very strong anti-oxidant and anti-aging effects.
Research shows that the main active principle that rainbow conk contains is polysaccharide, is the new immunomodulator of a class.Can significantly it carry
Height promotes bone-marrow-derived lymphocyte to produce antibody, enhancing NK cytotoxicities and M Φ inductions through the post-stimulatory T lymphocytic proliferation rates of PHA
Produce TNF (TNF), interleukin 1 (IL-1), interleukin 2 (IL-2) and gamma interferon.To ring phosphorus
Neuroleptic Leukocytopenia caused by acid amides has rebound significantly effect, and can activate the function of reticuloendothelial system, dramatically increases phagocytic index
With phagocytosis coefficient, Antiradiation injury.
Modern medicine study proves that coriolan and micro- Organic Selenium have obvious immunological regulation, antitumor, rush
Enter cytokine profiles generation, promote wounded hepatocytes to recover and improve the multiple pharmacological effects such as infirmities of age symptom, in recent years,
It is low, tumour auxiliary that coriolan and Organic Selenium are widely used in regulation immunologic function, Antiradiation injury, preventing and treating leucocyte by clinic
Help treatment etc..973 years, American scholar Rotruck etc. had found that selenium is the required component of glutathione peroxidase, i.e., no selenium
In the presence of this enzyme just without vigor, so as to disclose the biologically active form I of selenium.The same year, the World Health Organization announces：Selenium is people
It is micro- necessary to class.Selenium was also classified as essential nutrient element by Chinese Soclety of Nutrition in 1988, the selenium supply that China is recommended
Measure as 50-250 μ g/d.Keshan disease, Kaschin-Beck disease, cancer, cardiovascular and cerebrovascular disease, immunity of organisms decline and aging course are equal
It is relevant with selenium intake deficiency in meals.Selenium element is strengthened by food approach, it has also become the study hotspot of current functional food
One of.
The content of the invention
It is an object of the invention to provide a kind of selenium of selenium-methyl selenium containing L- for combining coriolan and micro- Organic Selenium
Coriolan for cysteine and preparation method thereof.
A kind of preparation method of the coriolan of selenium-methyl selenium substituted aminothiopropionic containing L-, comprises the following steps：
Step (1)：Rainbow conk is cleaned, rainbow conk is shredded；
Step (2)：Rainbow conk in step (1) is mixed with water, heating extraction, the removal of impurity is gone in filtering, obtains filtrate；
Step (3)：The ethanol of 3~4 times of filtrate volume of addition in the filtrate obtained by step (2), standing 20~30 hours,
Collect precipitation；
Step (4)：Precipitation obtained by step (3) is washed 3~5 times with ethanol；Alcohol precipitation is washed off according to similar compatibility molten
Liquid；
Step (5)：Precipitation obtained by step (4) is dried, crushed, 80~150 mesh sieves is crossed, produces coriolan；
Step (6)：Coriolan and L- selenium-methyl selenium substituted aminothiopropionics obtained by step (5) is pressed 10000:1 weight
Than well mixed.
The concrete operation step of the step (2) is：The rainbow conk of chopping is put into heating extraction in water, Extracting temperature is
100 DEG C, extraction time is two hours, filtering；Filter residue carries out second and extracted, second of extraction and first time extraction conditions phase
Together, filtered after second of extraction, merge filtrate twice；The amount of water extracted twice is 8-10 times of rainbow conk weight.
Described step (2) also includes：Vacuum distillation is carried out to the filtrate after merging, filtrate is concentrated into former filtrate volume
1/20-1/18, distillation pressure 0.02-0.03MPa, temperature control is at 60-70 DEG C.
Described step (3) also includes filtering the mode of the collection precipitation of gained for filter paper.
The concentration of alcohol added in described step (3) is 95% medicinal alcohol.
Ethanol in described step (4) is the ethanol that concentration is 70%.
Drying precipitated method is vacuum drying in described step (5).
A kind of coriolan of the selenium-methyl selenium substituted aminothiopropionic containing L-, the selenium-methyl selenium substituted aminothiopropionic containing L-
The content of polysaccharide is not less than 50% in coriolan.
The content of selenium is the μ g/g of 40 μ g/g~60 in the coriolan of the described selenium-methyl selenium substituted aminothiopropionic containing L-.
The content of L- selenium-methyl selenium substituted aminothiopropionics in the coriolan of the described selenium-methyl selenium substituted aminothiopropionic containing L-
For the μ g/g of 90 μ g/g~150.
Coriolan promotes antibody formation and complement to swash mainly by activating macrophage, T lymphocytes, bone-marrow-derived lymphocyte
Live and produce immunoregulation effect.Selenium is the activated centre of glutathione peroxidase, is used as glutathione peroxidase
(GSH-Px) necessary component, the removing to interior free yl and lipid peroxide plays main function, stablizes biomembrane
Required composition, also with very strong anti-oxidant and anti-aging effects.Technical scheme is by after coriolan and selenium compatibility
Organism immunoregulation effect can preferably be produced.
Embodiment
Embodiment 1
A kind of preparation method of the coriolan of selenium-methyl selenium substituted aminothiopropionic containing L-, comprises the following steps：
Step (1)：Rainbow conk is cleaned, rainbow conk is shredded；
Step (2)：Rainbow conk in step (1) is mixed with water, heating extraction, the removal of impurity is gone in filtering, obtains filtrate；
Step (3)：The ethanol of 3 times of filtrate volume is added in the filtrate obtained by step (2), stands 20 hours, collect heavy
Form sediment；
Step (4)：Precipitation obtained by step (3) is washed 3 times with ethanol；Alcohol precipitation solution is washed off according to similar compatibility；
Step (5)：Precipitation obtained by step (4) is dried, crushed, 80 mesh sieves is crossed, produces coriolan；
Step (6)：Coriolan and L- selenium-methyl selenium substituted aminothiopropionics obtained by step (5) is pressed 10000:1 weight
Than well mixed.
The concrete operation step of the step (2) is：The rainbow conk of chopping is put into heating extraction in water, Extracting temperature is
100 DEG C, extraction time is two hours, filtering；Filter residue carries out second and extracted, second of extraction and first time extraction conditions phase
Together, filtered after second of extraction, merge filtrate twice；The amount of water extracted twice is 8-10 times of rainbow conk weight.
Described step (2) also includes：Vacuum distillation is carried out to the filtrate after merging, filtrate is concentrated into former filtrate volume
1/20, distillation pressure 0.02MPa, temperature control is at 60 DEG C.
Described step (3) also includes filtering the mode of the collection precipitation of gained for filter paper.
The ethanol added in described step (3) is the medicinal alcohol that concentration is 95%.
Ethanol in described step (4) is the ethanol that concentration is 70%.
Drying precipitated method is vacuum drying in described step (5).
A kind of coriolan of the selenium-methyl selenium substituted aminothiopropionic containing L-, the selenium-methyl selenium substituted aminothiopropionic containing L-
The content of polysaccharide is not less than 50% in coriolan.
The content of selenium is 40 μ g/g in the coriolan of the described selenium-methyl selenium substituted aminothiopropionic containing L-.
The content of L- selenium-methyl selenium substituted aminothiopropionics in the coriolan of the described selenium-methyl selenium substituted aminothiopropionic containing L-
For 100 μ g/g.
Embodiment 2
A kind of preparation method of the coriolan of selenium-methyl selenium substituted aminothiopropionic containing L-, comprises the following steps：
Step (1)：Rainbow conk is cleaned, rainbow conk is shredded；
Step (2)：Rainbow conk in step (1) is mixed with water, heating extraction, the removal of impurity is gone in filtering, obtains filtrate；
Step (3)：The ethanol of 4 times of filtrate volume is added in the filtrate obtained by step (2), stands 30 hours, collect heavy
Form sediment；
Step (4)：Precipitation obtained by step (3) is washed 4 times with ethanol；Alcohol precipitation solution is washed off according to similar compatibility；
Step (5)：Precipitation obtained by step (4) is dried, crushed, 150 mesh sieves is crossed, produces coriolan；
Step (6)：Coriolan and L- selenium-methyl selenium substituted aminothiopropionics obtained by step (5) is pressed 10000:1.5 weight
Amount is than well mixed.
The concrete operation step of the step (2) is：The rainbow conk of chopping is put into heating extraction in water, Extracting temperature is
100 DEG C, extraction time is two hours, filtering；Filter residue carries out second and extracted, second of extraction and first time extraction conditions phase
Together, filtered after second of extraction, merge filtrate twice；The amount of water extracted twice is 10 times of rainbow conk weight.
Described step (2) also includes：Vacuum distillation is carried out to the filtrate after merging, filtrate is concentrated into former filtrate volume
1/10, distillation pressure 0.02MPa, temperature control is at 60 DEG C.
Described step (3) also includes filtering the mode of the collection precipitation of gained for filter paper.
The ethanol added in described step (3) is the medicinal alcohol that concentration is 95%.
Ethanol in described step (4) is the ethanol that concentration is 70%.
Drying precipitated method is vacuum drying in described step (5).
A kind of coriolan of the selenium-methyl selenium substituted aminothiopropionic containing L-, the selenium-methyl selenium substituted aminothiopropionic containing L-
The content of polysaccharide is not less than 50% in coriolan.
The content of selenium is 60 μ g/g in the coriolan of the described selenium-methyl selenium substituted aminothiopropionic containing L-.
The content of L- selenium-methyl selenium substituted aminothiopropionics in the coriolan of the described selenium-methyl selenium substituted aminothiopropionic containing L-
For 150 μ g/g.
Embodiment 3
A kind of preparation method of the coriolan of selenium-methyl selenium substituted aminothiopropionic containing L-, comprises the following steps：
Step (1)：Rainbow conk is cleaned, rainbow conk is shredded；
Step (2)：Rainbow conk in step (1) is mixed with water, heating extraction, the removal of impurity is gone in filtering, obtains filtrate；
Step (3)：The ethanol of 3 times of filtrate volume is added in the filtrate obtained by step (2), stands 24 hours, collect heavy
Form sediment；
Step (4)：Precipitation obtained by step (3) is washed 3 times with ethanol；Alcohol precipitation solution is washed off according to similar compatibility；
Step (5)：Precipitation obtained by step (4) is dried, crushed, 100 mesh sieves is crossed, produces coriolan；
Step (6)：Coriolan and L- selenium-methyl selenium substituted aminothiopropionics obtained by step (5) is pressed 10000:1.25
Weight is than well mixed.
The concrete operation step of the step (2) is：The rainbow conk of chopping is put into heating extraction in water, Extracting temperature is
100 DEG C, extraction time is two hours, filtering；Filter residue carries out second and extracted, second of extraction and first time extraction conditions phase
Together, filtered after second of extraction, merge filtrate twice；The amount of water extracted twice is 10 times of rainbow conk weight.
Described step (2) also includes：Vacuum distillation is carried out to the filtrate after merging, filtrate is concentrated into former filtrate volume
1/20, distillation pressure 0.01MPa, temperature control is at 70 DEG C.
Described step (3) also includes filtering the mode of the collection precipitation of gained for filter paper.
The ethanol added in described step (3) is the medicinal alcohol that concentration is 95%.
Ethanol in described step (4) is the ethanol that concentration is 70%.
Drying precipitated method is vacuum drying in described step (5).
A kind of coriolan of the selenium-methyl selenium substituted aminothiopropionic containing L-, the selenium-methyl selenium substituted aminothiopropionic containing L-
The content of polysaccharide is not less than 50% in coriolan.
The content of selenium is 50 μ g/g in the coriolan of the described selenium-methyl selenium substituted aminothiopropionic containing L-.
The content of L- selenium-methyl selenium substituted aminothiopropionics in the coriolan of the described selenium-methyl selenium substituted aminothiopropionic containing L-
For 125 μ g/g.
Organism immunoregulation effect can will be preferably produced in technical scheme after coriolan and selenium compatibility.
The coriolan of described L- selenium-methyl selenium substituted aminothiopropionics has effects that immunoloregulation function, low available for leucocyte,
The treatment of immunologic hypofunction and chronic hepatitis.
Claims (10)
1. the preparation method of the coriolan of a kind of selenium-methyl selenium substituted aminothiopropionic containing L-, it is characterised in that including following step
Suddenly：
Step (1)：Rainbow conk is cleaned, rainbow conk is shredded；
Step (2)：Rainbow conk in step (1) is mixed with water, heating extraction, the removal of impurity is gone in filtering, obtains filtrate；
Step (3)：The ethanol of 3~4 times of filtrate volume is added in the filtrate obtained by step (2), 20~30 hours is stood, collects
Precipitation；
Step (4)：Precipitation obtained by step (3) is washed 3~5 times with ethanol；
Step (5)：Precipitation obtained by step (4) is dried, crushed, 80~150 mesh sieves is crossed, produces coriolan；
Step (6)：Coriolan and L- selenium-methyl selenium substituted aminothiopropionics obtained by step (5) is pressed 10000:1~1.5 weight
Amount is than well mixed.
2. a kind of preparation method of the coriolan of selenium-methyl selenium substituted aminothiopropionic containing L- according to claim 1, it is special
Levy and be, the concrete operation step of the step (2) is：The rainbow conk of chopping is put into heating extraction in water, Extracting temperature is 100
DEG C, extraction time is two hours, filtering；Filter residue carries out second and extracted, and second of extraction is identical with first time extraction conditions, the
Filtered after second extraction, merge filtrate twice；The amount of water extracted twice is 8-10 times of rainbow conk weight.
3. a kind of preparation method of the coriolan of selenium-methyl selenium substituted aminothiopropionic containing L- according to claim 2, it is special
Levy and be, described step (2) also includes：Filtrate after merging is distilled, filtrate is concentrated into former filtrate volume
1/20-1/18, distillation can be with air-distillation also vacuum distillation, vacuum distillation, distillation pressure 0.02-0.03MPa, temperature
Control is at 60-70 DEG C.
4. a kind of preparation method of the coriolan of selenium-methyl selenium substituted aminothiopropionic containing L- according to claim 1, it is special
Levy and be, described step (3) also includes filtering the mode of the collection precipitation of gained for filter paper.
5. a kind of preparation method of the coriolan of selenium-methyl selenium substituted aminothiopropionic containing L- according to claim 1, it is special
Levy and be, the ethanol added in described step (3) is the medicinal alcohol that concentration is 95%.
6. a kind of preparation method of the coriolan of selenium-methyl selenium substituted aminothiopropionic containing L- according to claim 1, it is special
Levy and be, the ethanol in described step (4) is the ethanol that concentration is 70%.
7. a kind of preparation method of the coriolan of selenium-methyl selenium substituted aminothiopropionic containing L- according to claim 1, it is special
Levy and be, drying precipitated method is vacuum drying in described step (5).
8. the coriolan of a kind of selenium-methyl selenium substituted aminothiopropionic containing L-, it is characterised in that it is any according to claim 1~7
Preparation method described in, which makes, to be obtained.
9. a kind of coriolan of selenium-methyl selenium substituted aminothiopropionic containing L- according to claim 8, it is characterised in that institute
The content of polysaccharide is not less than 50% in the coriolan for the selenium-methyl selenium substituted aminothiopropionic containing L- stated.
10. a kind of coriolan of selenium-methyl selenium substituted aminothiopropionic containing L- according to claim 9, it is characterised in that institute
The content of selenium is the μ g/g of 40 μ g/g~60 in the coriolan for the selenium-methyl selenium substituted aminothiopropionic containing L- stated.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111543641A (en) * | 2020-04-27 | 2020-08-18 | 江苏惠利生物科技有限公司 | Method for improving stability and bioavailability of L-selenium-methyl selenocysteine |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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