CN104262453A - Gradient extraction method for plant protein - Google Patents
Gradient extraction method for plant protein Download PDFInfo
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- CN104262453A CN104262453A CN201410562291.3A CN201410562291A CN104262453A CN 104262453 A CN104262453 A CN 104262453A CN 201410562291 A CN201410562291 A CN 201410562291A CN 104262453 A CN104262453 A CN 104262453A
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Abstract
The invention provides a gradient extraction method for plant protein. The gradient extraction method is characterized by comprising the following steps: (1) performing quick extraction, namely adding water into skimmed protein meal for quick extraction, performing solid-liquid separation, and desugaring, concentrating and drying supernatant liquid to prepare protein; (2) performing secondary extraction, namely adding water into precipitates obtained in the step (1) for secondary extraction, and performing solid-liquid separation; (3) performing acid precipitation, namely performing acid precipitation on the supernatant liquid obtained in the step (2), performing solid-liquid separation, re-dissolving the solid obtained by acid precipitation through a re-dissolving buffer solution, concentrating, desalting, drying, and combining with protein prepared in the step (1) to obtain plant isolated protein; (4) preparing a feed, namely adding water into the solid separated in the step (3), adjusting the pH value to be 7, and drying to obtain the feed. As difficultly precipitated small-molecular-weight protein in whey wastewater is early extracted in a conventional method, the COD (chemical oxygen demand) value of the generated whey wastewater is low. According to the gradient extraction method, the process is simple and easy to control, the energy consumption can be effectively reduced, and the production cost can be lowered.
Description
Technical field
The present invention relates to technical field of biochemical industry, particularly the technology of preparing of protein in the defatted vegetable dregs of rice
Background technology
Vegetable-protein has the characteristic such as aboundresources, cheap and easy to get and unique physiological function, has become the focus of food research exploitation.Research has found that vegetable-protein has following physiological function: 1) to the nutritional support effect of liver cirrhosis patient, 2) reduce cholesterol levels, 3) reduce the generation of cancer, 4) improve the symptom etc. of nephropathy and cardiovascular disorder.Isolated plant protein from the defatted vegetable dregs of rice, can improve Dietary Pattern of Residents, provides new protein source, for Traditional Agricultural Product processing industry provides new growth engines, have good economic benefit and social benefit.
At present, what domestic and international most plants protein isolate manufacturer adopted is alkali extraction-acid precipitation.This technique mainly utilizes dilute alkaline soln to make proteolytic in low-temperature defatted plant waste matter out, centrifugal removing insoluble solid residue, the pH value of extracting solution is adjusted to about 4.6 by recycling acid, make protein group fractional precipitation, and then obtain plant separation protein powder through techniques such as centrifugal, washing, neutralization, sterilizing and spraying dry.The whey wastewater COD that such process energy consumption is high, water consumption is high, produce is high, and research and development protein separating technology is rapidly and efficiently imperative.
Existing protein separating technology research and development mainly concentrate on and use novel Extraction solvent aspect.As patent (CN201280035564.8) uses watersoluble chlorinated calcium solution to extract soybean protein from soybean, soy protein products is obtained after concentrated, diafiltration, drying, soybean protein prepared by the method can be dissolved in acidic medium, but protein extracting ratio is low, soybean protein resource can not be made full use of.For another example patent (CN201210097106.9) utilizes normal hexane and ethanol two kinds of solvents to extract soybean protein, degreasing can be realized, desugar is disposable completes, improve effect of extracting, increase albumen yield, but with an organic solvent environmental pollution is serious, is unfavorable for amplifying and produces.
Summary of the invention
The object of the invention is to provide a kind of vegetable-protein gradient extraction technology, utilizes the difference of protein dissolution rate in the defatted vegetable dregs of rice to realize the separation of protein.First with water, rapid extraction is carried out to the defatted vegetable dregs of rice, by protein extraction fast for dissolution rate out, obtain albumen through desugar, concentrated, drying; Secondly extract solid residue basic solution remaining after rapid extraction, the albumen obtained with rapid extraction after Acid precipitation, drying merges and is high purity protein product, and finally, remaining solid residue carries out drying and crushing, prepares feeds product.The method operating procedure simply, easily controls, and prepares high-purity protein product by gradient fractionation, can effective energy efficient, and reduce production cost, the whey wastewater COD value of generation is low.
The object of the present invention is achieved like this: with the defatted vegetable dregs of rice for raw material, and obtain high purity protein product by rapid extraction, second extraction, drying and other steps, concrete grammar step is as follows:
1) rapid extraction
In defatted vegetable dregs of rice weight: water volume ratio is the ratio of 1:4 ~ 1:10, is positioned in container by the defatted vegetable dregs of rice and water, at 25 ~ 85 DEG C, stirring and leaching 3 ~ 20 minutes, has extracted rear centrifugation, collects supernatant liquor and precipitation respectively.Supernatant liquor obtains albumen through desugar, concentrated, drying.
In specific implementation process, the described defatted vegetable dregs of rice include but not limited to peanut meal, soybean meal, sesame seed meal, the corn dregs of rice, the wheatgerm dregs of rice.
In specific implementation process, preferably, the weightmeasurement ratio 1:5 ~ 1:8 pressing g/ml of the described defatted vegetable dregs of rice and water, described extraction temperature is 30 DEG C ~ 80 DEG C, and described extraction time is 5 ~ 15 minutes.
2) second extraction
In Sediment weight: water volume ratio is the ratio of 1:3 ~ 1:8, by step 1) in the precipitation of collecting and water be positioned in container, at 25 ~ 85 DEG C, stirring and leaching 20 ~ 70 minutes, has extracted rear centrifugation, respectively collection supernatant liquor and precipitation.
In specific implementation process, preferably described step 1) weightmeasurement ratio by g/ml of the solid that is separated and water is 1:4 ~ 1:6, described extraction temperature is 30 DEG C ~ 80 DEG C, and described extraction time is 30 ~ 60 minutes.
3) Acid precipitation
By step 2) supernatant liquor collected imports in container, and adjust pH 3.6 ~ 4.6 to precipitate, centrifugation after precipitation, collects supernatant liquor and precipitation respectively.By Sediment weight: liquor capacity is than being 1:10 ~ 1:50, the phosphate buffered saline buffer of 10 ~ 500mMpH6.5 ~ 8.0 is added precipitation, redissolves, spraying dry after ultrafiltration and concentration desalination, with step 1) in the albumen that obtains merge, as defatted vegetable protein product.
In specific implementation process, preferably, by step 2) liquid pH value that is separated is adjusted to 3.5 ~ 4.6, described redissolution damping fluid is 20 ~ 200mM, pH value is the phosphate buffered saline buffer of 6.5 ~ 7.5, and the weightmeasurement ratio by g/ml of described Acid precipitation gained solid and described phosphate buffered saline buffer is 1:20 ~ 1:40.
4) feed preparation
By step 3) precipitation of collecting adds the water of 5 ~ 20 times of volumes, and adjust pH to neutral, carry out spraying dry and obtain animal food prods.
In specific implementation process, preferably, described solid and the water added are 1:7 ~ 1:15 by the weightmeasurement ratio of g/ml, and adjust ph is 7.0.
Compared with prior art, the defatted vegetable cake protein gradient extraction technology that present method provides has the following advantages:
(1) defatted vegetable dregs of rice comprehensive utilization ratio is high.Adopt the method for gradient separations, albumen fast for dissolution rate is first extracted, after desugar, concentrated, drying, make albumen, then by other protein extraction out, merge as plant protein products;
(2) method solvent usage quantity is few, and not with an organic solvent, the albumen good due to solubility property is separated in rapid extraction step, and in the heavy whey wastewater produced of second step acid, protein content is very low, greatly reduces COD value, environmental friendliness, low stain;
(3) process stabilizing, easy and simple to handle, Standardization instrument, with low cost, easy to utilize.
Adopt the inventive method can fully utilize defatted vegetable dregs of rice resource, prepare the plant protein products of high added value, the industries such as food, nutritive health-care, biochemistry, daily use chemicals can be widely used in, be conducive to the Sustainable development of traditional food industry, be conducive to the competitive power of raising industry.
Embodiment
Referring to specific embodiment, the present invention is described.It will be appreciated by those skilled in the art that these embodiments are only for illustration of the present invention, its scope do not limited the present invention in any way.
Embodiment 1
1) taking defatted peanut dregs of rice 10kg is positioned in stirred pot, and add water 50L, stirring and leaching 5 minutes at 30 DEG C, has extracted rear 10000g centrifugal 15 minutes, collects supernatant liquor and precipitation respectively, obtains supernatant liquor 38.5L, precipitation 19.1kg.
2) supernatant liquor carried out desugar, concentrate with the ultra-filtration membrane of molecular weight cut-off 3kDa, spraying dry obtains protein 31 8.4g respectively.
3) by step 1) the middle 19.1kg collected precipitates and 76.4L water is positioned in container, and stirring and leaching 30 minutes at 30 DEG C, has extracted rear 10000g centrifugal 15 minutes, collects supernatant liquor and precipitation respectively.
4) by step 3) in the supernatant liquor collected import in the heavy still of acid, adjust pH 4.0 to precipitate, after precipitation half an hour, centrifugal 15 minutes of 10000g, collects supernatant liquor and precipitation respectively, is precipitated 96.1g.The phosphate buffered saline buffer of 961ml20mM pH7.0 is added precipitation, redissolves, after ultrafiltration and concentration desalination, spraying dry obtains protein 94 .2g, and the albumen obtained with step 2 merges, and obtains 414.5g types peanut protein product altogether.
5) by step 3) precipitation of collecting, add the water of 3 times of volumes, adjust pH to neutral, carry out spraying dry and obtain 9.2kg feeds product.
Embodiment 2
1) taking de-fatted soybean dregs 10kg is positioned in stirred pot, and add water 80L, stirring and leaching 15 minutes at 80 DEG C, has extracted rear 10000g centrifugal 15 minutes, collects supernatant liquor and precipitation respectively, obtains supernatant liquor 56.3L, precipitation 20.2kg.
2) supernatant liquor carried out desugar, concentrate with the ultra-filtration membrane of molecular weight cut-off 3kDa, spraying dry obtains protein 42 1.9g respectively.
3) by step 1) the middle 20.2kg collected precipitates and 121.2L water is positioned in container, and stirring and leaching 60 minutes at 80 DEG C, has extracted rear 10000g centrifugal 15 minutes, collects supernatant liquor and precipitation respectively.
4) by step 3) in the supernatant liquor collected import in the heavy still of acid, adjust pH 4.3 to precipitate, after precipitation half an hour, centrifugal 15 minutes of 10000g, collects supernatant liquor and precipitation respectively, is precipitated 135.4g.The phosphate buffered saline buffer of 1354ml20mM pH7.0 is added precipitation, redissolves, after ultrafiltration and concentration desalination, spraying dry obtains albumen 133.8g, and the albumen obtained with step 2 merges, and obtains 555.7g soy protein products altogether.
5) by step 3) precipitation of collecting, add the water of 3 times of volumes, adjust pH to neutral, carry out spraying dry and obtain 9.1kg feeds product.
Embodiment 3
1) taking degreasing walnut dregs of rice 10kg is positioned in stirred pot, and add water 50L, stirring and leaching 10 minutes at 45 DEG C, has extracted rear 10000g centrifugal 15 minutes, collects supernatant liquor and precipitation respectively, obtains supernatant liquor 37.6L, precipitation 20.3kg.
2) supernatant liquor carried out desugar, concentrate with the ultra-filtration membrane of molecular weight cut-off 3kDa, spraying dry obtains protein 37 2.5g respectively.
3) by step 1) the middle 20.3kg collected precipitates and 101.5L water is positioned in container, and stirring and leaching 45 minutes at 50 DEG C, has extracted rear 10000g centrifugal 15 minutes, collects supernatant liquor and precipitation respectively.
4) by step 3) in the supernatant liquor collected import in the heavy still of acid, adjust pH 4.6 to precipitate, after precipitation half an hour, centrifugal 15 minutes of 10000g, collects supernatant liquor and precipitation respectively, is precipitated 88.4g.The phosphate buffered saline buffer of 884ml20mM pH7.0 is added precipitation, redissolves, after ultrafiltration and concentration desalination, spraying dry obtains albumen 87.2g, and the albumen obtained with step 2 merges, and obtains 459.7g types peanut protein product altogether.
5) by step 3) precipitation of collecting, add the water of 3 times of volumes, adjust pH to neutral, carry out spraying dry and obtain 9.4kg feeds product.
Applicant states, the present invention illustrates technical scheme of the present invention by above-described embodiment, but the present invention is not limited to above-described embodiment, does not namely mean that the present invention must rely on above-described embodiment and could implement.Person of ordinary skill in the field should understand, any improvement in the present invention, to equivalence replacement and the interpolation of ancillary component, the concrete way choice etc. of raw material selected by the present invention, all drops within protection scope of the present invention and open scope.
Claims (6)
1. a vegetable-protein gradient extraction method, is characterized in that, comprising:
1) rapid extraction: the apolipoprotein dregs of rice are added water and carries out quick lixiviate, solid-liquid separation, supernatant liquor prepares albumen through desugar, concentrated, drying;
2) second extraction: to step 1) in the precipitation that obtains add water and carry out secondary lixiviate, solid-liquid separation;
3) Acid precipitation: to step 2) in the supernatant liquor that obtains carry out Acid precipitation, solid-liquid separation, uses redissolution damping fluid to redissolve to Acid precipitation gained solid, then through concentrated, desalination, drying, with step 1) albumen prepared merges, and obtains plant separation protein;
4) feed is prepared: by step 3) solid that is separated adds water, and regulates pH neutral, dry, obtains feeds product.
2. vegetable-protein gradient extraction method as claimed in claim 1, is characterized in that, step 1) in, the described defatted vegetable dregs of rice include but not limited to peanut meal, soybean meal, sesame seed meal, the corn dregs of rice, the wheatgerm dregs of rice.
3. vegetable-protein gradient extraction method as claimed in claim 1 or 2, it is characterized in that, step 1) in, the weightmeasurement ratio by g/ml of the described defatted vegetable dregs of rice and water is 1:4 ~ 1:10, described extraction temperature is 25 DEG C ~ 85 DEG C, and described extraction time is 3 ~ 20 minutes;
Preferably, the weightmeasurement ratio 1:5 ~ 1:8 pressing g/ml of the described defatted vegetable dregs of rice and water, described extraction temperature is 30 DEG C ~ 80 DEG C, and described extraction time is 5 ~ 15 minutes.
4. vegetable-protein gradient extraction method according to claim 1 and 2, it is characterized in that, step 2) in, described step 1) weightmeasurement ratio by g/ml of the solid that is separated and water is 1:(3 ~ 8), described extraction temperature is 25 DEG C ~ 85 DEG C, and described extraction time is 20 ~ 70 minutes;
Preferably, described step 1) weightmeasurement ratio by g/ml of the solid that is separated and water is 1:4 ~ 1:6, described extraction temperature is 30 DEG C ~ 80 DEG C, and described extraction time is 30 ~ 60 minutes.
5. vegetable-protein gradient extraction method according to claim 1 and 2, it is characterized in that, step 3) in, described Acid precipitation is specially: by step 2) liquid pH value that is separated is adjusted to 3.0 ~ 5.0, described redissolution damping fluid is the phosphate buffered saline buffer of 10 ~ 500mM, pH6.5 ~ 8.0, and the weightmeasurement ratio by g/ml of described Acid precipitation gained solid and described phosphate buffered saline buffer is 1:10 ~ 1:50;
Preferably, by step 2) liquid pH value that is separated is adjusted to 3.5 ~ 4.6, described redissolution damping fluid is 20 ~ 200mM, pH value is the phosphate buffered saline buffer of 6.5 ~ 7.5, and the weightmeasurement ratio by g/ml of described Acid precipitation gained solid and described phosphate buffered saline buffer is 1:20 ~ 1:40.
6. vegetable-protein gradient extraction method according to claim 1 and 2, is characterized in that, by step 4) in be separated solid add water, described solid and the water added are 1:5 ~ 1:20 by the weightmeasurement ratio of g/ml, adjust ph to 6.5 ~ 7.5, spraying dry, obtains feeds product;
Preferably, described solid and the water added are 1:7 ~ 1:15 by the weightmeasurement ratio of g/ml, and adjust ph is 7.0.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106417885A (en) * | 2016-10-11 | 2017-02-22 | 重庆多典食品有限公司 | Extracting method of plant proteins |
| CN111202234A (en) * | 2020-01-14 | 2020-05-29 | 临沂金锣文瑞食品有限公司 | Vegetable protein sausage capable of effectively reducing beany flavor and bitter taste and preparation method thereof |
| CN111793121A (en) * | 2020-07-23 | 2020-10-20 | 临沂山松生物制品有限公司 | Separation and purification process of function specific protein |
| CN112552371A (en) * | 2020-12-24 | 2021-03-26 | 常州市芙丽佳生物科技有限公司 | Method for preparing olive kernel extract based on extraction and purification technology |
| CN113412874A (en) * | 2021-02-01 | 2021-09-21 | 黑龙江省科学院高技术研究院 | Extraction method of hemp seed storage protein |
-
2014
- 2014-10-21 CN CN201410562291.3A patent/CN104262453A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106417885A (en) * | 2016-10-11 | 2017-02-22 | 重庆多典食品有限公司 | Extracting method of plant proteins |
| CN111202234A (en) * | 2020-01-14 | 2020-05-29 | 临沂金锣文瑞食品有限公司 | Vegetable protein sausage capable of effectively reducing beany flavor and bitter taste and preparation method thereof |
| CN111793121A (en) * | 2020-07-23 | 2020-10-20 | 临沂山松生物制品有限公司 | Separation and purification process of function specific protein |
| CN112552371A (en) * | 2020-12-24 | 2021-03-26 | 常州市芙丽佳生物科技有限公司 | Method for preparing olive kernel extract based on extraction and purification technology |
| CN113412874A (en) * | 2021-02-01 | 2021-09-21 | 黑龙江省科学院高技术研究院 | Extraction method of hemp seed storage protein |
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Application publication date: 20150107 |