CN106434814A - Method for preparing compounded antihypertensive peptide through marine organisms - Google Patents
Method for preparing compounded antihypertensive peptide through marine organisms Download PDFInfo
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Abstract
The invention relates to a method for preparing a compounded antihypertensive peptide through marine organisms. The method includes the steps that 5%-10% by weight of trepang, 50%-65% by weight of jellyfishes, 10%-25% by weight of kelp and 10%-20% by weight of acetes chinensis are weighed and serve as raw materials, and the raw materials are desalted and softened respectively; then the raw materials are pulped respectively to be watered and ground into pulp; microorganisms are removed in a heated mode; cooling is carried out, then enzymolysis treatment is carried out, and enzyme denaturing is carried out; the product is cooled to be centrifuged, liquid supernatant is taken, and macromolecules are filtered and removed through a tubular membrane; the filtered liquid is graded by intercepting an organic membrane with the molecular mass of 3000Dalton, intercepted liquid with the molecular mass below 3000Dalton is collected, subjected to nanofiltration, concentrated and desalted, dried and compounded, and the compounded antihypertensive peptide is obtained. The best optimized enzymolysis technical formula is determined in the method; as pipe filtration, ultrafiltration and nanofiltration separation and extraction technologies are adopted, the extracting efficiency is high, and the obtained compounded antihypertensive peptide is remarkable in blood-pressure reduction effect. The method is scientific in material selecting, reasonable in technology and easy and convenient to operate, and industrial production is easy to achieve.
Description
Technical field
The present invention relates to a kind of preparation of peptide, especially a kind of method producing composite blood pressure lowering peptide using marine organisms.
Background technology
Blood pressure lowering peptide, be also called angiotensin converting enzyme (Angiotensin Converting Enzyme,
EC3.4.15.1, abbreviation ACE) peptide for inhibiting is that the class isolated from food proteins has and significantly reduces blood pressure effect
Peptide, usually small-molecular peptides.In numerous depressor substances, blood pressure lowering peptide becomes bioactive peptide because of the buck functionality that it has no toxic side effect
The focus of research.Detached blood pressure lowering peptide from natural food albumen although antihypertensive effect be not so good as synthetic drug so strong, but
It has safe, only plays hypotensive effect to hyperpietic, to normotensive's no hypotensive effect, does not have blood pressure lowering
Degree phenomenon occurs.The present casein from milk casein, lactalbumin etc.；Carnis Gallus domesticus in animal and vegetable protein, gelatin, pig
Meat, egg, Semen Fagopyri Esculenti, Semen Maydiss, Broccoli, Bulbus Allii etc.；Sardine in fish shellfish algae protein, tuna, Caput Monopteri albi, Concha Ostreae, opotism
ACE inhibitor is isolated in the numerous food products such as dish；Domestic researcher also from the food Ji Me Lao Jiu of Chinese tradition, in fermented bean curd
Find ace inhibitory peptide.
Modern medicine study proves, Stichopus japonicuss be rich in polysaccharide, holothurin, holotoxin, glutamic acid, glycine, arginine,
Selenium, zinc, ferrum, iodine, calcium, superoxide dismutase（SOD）, more than the 50 kind of nutritional labeling beneficial to human body such as chondroitin sulfate.Warp
Measuring, Stichopus japonicuss range of hydrolysed peptides also has certain ACE inhibitory activity, is potential material for lowering blood pressure.In addition, Rhopilema esculenta species
Many, quantity is big, and in China, distribution is wide, is the important component part of marine zooplankton group.Containing higher egg in Rhopilema esculenta body
The inorganic salt such as white matter and calcium, phosphorus, iodine, ferrum, have the laudatory title of " Aquatic product rarity ", and Chinese medicine thinks, Rhopilema esculenta has expansion of blood vessels, fall
The effect of blood pressure.In addition, Chinese green food is the peculiar species of China, China coast is all distributed, especially most with Sea And Bohai Sea Coast yield.
And in the aminoacid in Acetess, containing the branched-chain amino acid relevant with blood pressure lowering peptide structure and aromatic amino acid, Chinese green food is
The very good material that depressor substance extracts.In addition, Thallus Laminariae (Thallus Eckloniae) contains phycocolloid acid, laminaran, the polysaccharide such as galactan, laminine,
Glutamic acid, aspartic acid, the aminoacid such as proline, vitamin B1, B2, C, P and carotene, the inorganic salt such as iodine, potassium, calcium.In
Doctor thinks, Thallus Laminariae (Thallus Eckloniae) have hard masses softening and resolving, eliminating phlegm to relieve asthma, current diuretic, dispel lipoid and reducing blood pressure the effects such as.Modern medicine study shows, from
The laminine extracting in Thallus Laminariae (Thallus Eckloniae) and Mannitol have hypotensive effect.
At present, the domestic research with regard to blood pressure lowering peptide is concentrated mainly on the isolating and purifying of peptide, Structural Identification and activity rating
Aspect.How to determine enzymatic hydrolysis condition that is optimal, optimizing and blood pressure lowering peptide is improved separation and Extraction efficiency be one urgently to be resolved hurrily
Technical problem.Meanwhile, at present, yet there are no the research report producing composite blood pressure lowering peptide using multiple marine organisms.
Content of the invention
In order to overcome the deficiencies in the prior art, the present invention provides a kind of side producing composite blood pressure lowering peptide using marine organisms
Method.The raw material of the method chooses science, rational technology, easy and simple to handle, workable, separation and Extraction efficiency high, easily realizes work
Industry metaplasia is produced.Produce the composite blood pressure lowering peptide of gained according to the method, there is good antihypertensive effect.
The technical solution adopted for the present invention to solve the technical problems is：One kind produces compound blood pressure lowering using marine organisms
The method of peptide, it is to choose Stichopus japonicuss, Rhopilema esculenta, Thallus Laminariae (Thallus Eckloniae) and Chinese green food for raw material, weighs by weight percentage：Stichopus japonicuss 5～10%,
Rhopilema esculenta 50～65%, Thallus Laminariae (Thallus Eckloniae) 10～25% and Chinese green food 10～20% carry out desalination, sofening treatment respectively；Again respectively after beating
The pure water of 10～30 times of addition is crossed colloid and is milled into slurry；Slurry heating is gone out except microorganism；Carry out at enzymolysis after cooling
Reason, heats enzyme denaturing；After cooling, centrifugation removes insoluble matter, takes supernatant to pass through tubular membrane filtering and removing macromole；Filtered solution passes through
Trapped molecular weight is the organic membrane classification of 3000Dalton, collects the trapped fluid of below 3000Dalton；Trapped fluid nanofiltration is dense
Contracting desalination, is dried, and compounds, obtains composite blood pressure lowering peptide.
The present invention chooses Stichopus japonicuss, Rhopilema esculenta, Thallus Laminariae (Thallus Eckloniae) and Chinese green food are raw material, by each raw material respectively through desalination, softening at
Reason, more respectively through the defibrination that adds water, heating is gone out except microorganism；Digest after cooling, enzyme denaturing；Centrifugation, take supernatant to filter through pipe respectively again,
Ultrafiltration, nanofiltration, are dried, and compound, obtain composite blood pressure lowering peptide.The selection that the present invention is scientific and reasonable have buck functionality Stichopus japonicuss,
Rhopilema esculenta, Thallus Laminariae (Thallus Eckloniae) and Chinese green food are raw material, will have the small-molecular peptides of ACE inhibitory activity through enzymolysis, pipe filter, ultrafiltration, nanofiltration
Separate, and save its intact structure and effective biological activity.Experiment shows, the fall blood isolated using this technique
After pressure peptide components compound, through original hypertensive rat experiment, rat diastolic pressure can be significantly decreased.Determine in the method
The zymolysis technique scheme of good optimization, the pipe filter being adopted, ultrafiltration, nanofiltration separation extractive technique so as to extract efficiency high.This
Bright produces the method rational technology of composite blood pressure lowering peptide, easy and simple to handle, workable, easily realization industry using marine organisms
Metaplasia is produced.
A kind of method producing composite blood pressure lowering peptide using marine organisms, its concrete technology step is as follows：
It is raw material that a, the selection of raw material choose Stichopus japonicuss, Rhopilema esculenta, Thallus Laminariae (Thallus Eckloniae) and Chinese green food with process, weighs by weight percentage：Sea
Ginseng 5～10%, Rhopilema esculenta 50～65%, Thallus Laminariae (Thallus Eckloniae) 10～25% and Chinese green food 10～20% are respectively put into immersion more than 24h in cold water, go
Except salinity, clean, pull out and drain away the water, carry out making beating process using beater respectively, add 10～30 times of pure water afterwards
Using colloid mill by material micronization processes, obtain Stichopus japonicuss slurry, Rhopilema esculenta slurry, Thallus Laminariae (Thallus Eckloniae) slurry and Chinese green food slurry；
B, enzymolysis are by the Stichopus japonicuss slurry obtained by step (a), Rhopilema esculenta slurry, Thallus Laminariae (Thallus Eckloniae) slurry and Chinese green food slurry
It is heated to Solutions Center temperature and is 95～100 DEG C, keep 10min, go out except microorganism；Then carry out enzymolysis processing, wherein respectively starch
Shape thing enzymolysis condition be：
By Stichopus japonicuss slurry temperature adjustment to 50～55 DEG C of addition compound proteases, its consumption is 3～the 10 ‰ of Stichopus japonicuss weight, enzyme
After solution 1～2h, add flavor protease, its consumption is 2～the 10 ‰ of Stichopus japonicuss weight, digests 2～3h, obtains Apostichopus enzymolysis liquid；
By Rhopilema esculenta slurry temperature adjustment to 35～40 DEG C, adjust pH value 3～3.5, add pepsin, its consumption is Rhopilema esculenta weight
2～the 5 ‰ of amount, after enzymolysis 2～5h, adjust temperature and are 45～50 DEG C, pH value is 5～5.5, adds papain, its consumption
For Rhopilema esculenta weight 4～8 ‰, digest 1～3h, obtain Rhopilema esculenta enzymolysis solution；
By Thallus Laminariae (Thallus Eckloniae) slurry temperature adjustment to 45～55 DEG C, add cellulase, pectase and compound protease, digest 3～4h,
Obtain Thallus Laminariae (Thallus Eckloniae) enzymolysis solution；Wherein, the consumption of described cellulase be 4～the 8 ‰ of Thallus Laminariae (Thallus Eckloniae) weight, the consumption of pectase be that Thallus Laminariae (Thallus Eckloniae) weighs
2～the 6 ‰ of amount, the consumption of compound protease is 3～the 10 ‰ of Thallus Laminariae (Thallus Eckloniae) weight；
By Chinese green food slurry temperature adjustment to 45～55 DEG C, adjusting pH value is 9.5～10, adds alkaline protease, its use
Measure for Chinese green food weight 3～7 ‰, enzymolysis 1～2h after, adjust pH value be 6.5～7, add flavor protease, its consumption
For Chinese green food weight 2～10 ‰, digest 1～2h, obtain Chinese green food enzymolysis solution；
Apostichopus enzymolysis liquid obtained by step (b), Rhopilema esculenta enzymolysis solution, Thallus Laminariae (Thallus Eckloniae) enzymolysis solution, Chinese green food enzymolysis solution are divided by c, enzyme denaturing
It is not heated to Solutions Center temperature and is 95～100 DEG C, keep 10min, carry out destroy the enzyme treatment, be subsequently cooled to 4 DEG C, stood
Night；
Enzyme denaturing that step (c) is obtained by d, centrifugation, cooling, the Apostichopus enzymolysis liquid standing, Rhopilema esculenta enzymolysis solution, Thallus Laminariae (Thallus Eckloniae) enzymolysis solution, in
State's Acetess enzymolysis solution is respectively adopted centrifuge and removes insoluble matter, and collects supernatant；
E, membrane filtration：Stichopus japonicuss that step (d) is obtained, Rhopilema esculenta, Thallus Laminariae (Thallus Eckloniae), Chinese green food supernatant pass through tubular type membrane filtration respectively
Removing macromole, the organic membrane retention classification that its periosteum filtered solution is 3000Dalton by pore size more respectively, collect
Below 3000Dalton trapped fluid；The trapped fluid of its below 3000Dalton passes through nanofiltration concentrating and desalinating, obtains nanofiltration retentate fluid；
F, drying：Stichopus japonicuss that step (e) is obtained, Rhopilema esculenta, Thallus Laminariae (Thallus Eckloniae), Chinese green food nanofiltration retentate fluid are dried, and compound, powder processed, obtain
Composite blood pressure lowering peptide.
Specific embodiment
With reference to embodiment, the present invention will be further described.
Embodiment 1
A kind of method producing composite blood pressure lowering peptide using marine organisms, its concrete technology step is as follows：
It is raw material that a, the selection of raw material and process weigh Stichopus japonicuss 10kg, Rhopilema esculenta 50kg, Thallus Laminariae (Thallus Eckloniae) 25kg and Chinese green food 15kg, point
Do not put into immersion 36h in cold water, desalination, clean, pull out and drain away the water, carry out making beating process using beater respectively, it
The pure water adding 20 times afterwards adopts colloid mill by material micronization processes, obtains Stichopus japonicuss slurry, Rhopilema esculenta slurry, Thallus Laminariae (Thallus Eckloniae) slurry
With Chinese green food slurry；
B, enzymolysis are by the Stichopus japonicuss slurry obtained by step (a), Rhopilema esculenta slurry, Thallus Laminariae (Thallus Eckloniae) slurry and Chinese green food slurry
It is heated to Solutions Center temperature and is 100 DEG C, keep 10min, go out except microorganism；Then enzymolysis processing, wherein each slurry are carried out
Enzymolysis condition be：
By Stichopus japonicuss slurry temperature adjustment to 55 DEG C of addition compound protease 90g, after enzymolysis 1h, add flavor protease 20g, enzyme
Solution 3h, obtains Apostichopus enzymolysis liquid；Wherein compound protein hydrolytic enzyme can be commercial commercial enzyme, such as Protamex compound protease,
Can voluntarily prepare, in the compound protein hydrolytic enzyme of preparation, contain papain and amylase.The present embodiment uses certainly
Row prepares compound protein hydrolytic enzyme；
By Rhopilema esculenta slurry temperature adjustment to 37 DEG C, adjust pH value 3, add pepsin 250g, after enzymolysis 2h, adjusting temperature is
50 DEG C, pH value is 5, adds papain 200g, digests 2h, obtains Rhopilema esculenta enzymolysis solution；
By Thallus Laminariae (Thallus Eckloniae) slurry temperature adjustment to 50 DEG C, add cellulase 200g, pectase 100g and compound protease 250g, enzyme
Solution 3h, obtains Thallus Laminariae (Thallus Eckloniae) enzymolysis solution；Wherein compound protein hydrolytic enzyme uses voluntarily preparation compound protein hydrolytic enzyme；
By Chinese green food slurry temperature adjustment to 50 DEG C, adjusting pH value is 10, adds alkaline protease 75g, after enzymolysis 1.5h,
Adjusting pH value is 6.5, adds flavor protease 150g, digests 1.5h, obtain Chinese green food enzymolysis solution；
Apostichopus enzymolysis liquid obtained by step (b), Rhopilema esculenta enzymolysis solution, Thallus Laminariae (Thallus Eckloniae) enzymolysis solution, Chinese green food enzymolysis solution are divided by c, enzyme denaturing
It is not heated to Solutions Center temperature and is 100 DEG C, keep 10min, carry out destroy the enzyme treatment, be subsequently cooled to 4 DEG C, stand overnight；
Enzyme denaturing that step (c) is obtained by d, centrifugation, cooling, the Apostichopus enzymolysis liquid standing, Rhopilema esculenta enzymolysis solution, Thallus Laminariae (Thallus Eckloniae) enzymolysis solution, in
State's Acetess enzymolysis solution is respectively adopted link-suspended basket centrifuge, controls rotating speed 2000 rpm, centrifugation removes insoluble matter, and collects supernatant
Liquid；
E, membrane filtration：Stichopus japonicuss that step (d) is obtained, Rhopilema esculenta, Thallus Laminariae (Thallus Eckloniae), Chinese green food supernatant pass through tubular type membrane filtration respectively
Removing macromole, the organic membrane retention classification that its periosteum filtered solution is 3000Dalton by pore size more respectively, collect
Below 3000Dalton trapped fluid；The trapped fluid of its below 3000Dalton passes through nanofiltration concentrating and desalinating, obtains nanofiltration retentate fluid；
F, drying：Stichopus japonicuss that step (e) is obtained, Rhopilema esculenta, Thallus Laminariae (Thallus Eckloniae), Chinese green food nanofiltration retentate fluid are spray-dried, and compound, and pulverize
Cheng Fen, obtains composite blood pressure lowering peptide.
Embodiment 2
A kind of method producing composite blood pressure lowering peptide using marine organisms, its concrete technology step is as follows：
It is raw material that a, the selection of raw material and process weigh Stichopus japonicuss 5kg, Rhopilema esculenta 65kg, Thallus Laminariae (Thallus Eckloniae) 10kg and Chinese green food 20kg, respectively
Put into immersion 48h in cold water, desalination, clean, pull out and drain away the water, carry out making beating process using beater respectively, afterwards
Add 10 times pure water adopt colloid mill by material micronization processes, obtain Stichopus japonicuss slurry, Rhopilema esculenta slurry, Thallus Laminariae (Thallus Eckloniae) slurry and
Chinese green food slurry；
B, enzymolysis are by the Stichopus japonicuss slurry obtained by step (a), Rhopilema esculenta slurry, Thallus Laminariae (Thallus Eckloniae) slurry and Chinese green food slurry
It is heated to Solutions Center temperature and is 95 DEG C, keep 10min, go out except microorganism；Then carry out enzymolysis processing, wherein each slurry enzyme
Solution condition be：
By Stichopus japonicuss slurry temperature adjustment to 50 DEG C of addition compound protease 25g, after enzymolysis 2h, add flavor protease 25g, enzyme
Solution 2.5h, obtains Apostichopus enzymolysis liquid；Wherein compound protein hydrolytic enzyme can be commercial Protamex compound protease；
By Rhopilema esculenta slurry temperature adjustment to 35 DEG C, adjust pH value 3.3, add pepsin 195g, after enzymolysis 3h, adjust temperature
For 45 DEG C, pH value is 5.5, adds papain 325g, digests 3h, obtains Rhopilema esculenta enzymolysis solution；
By Thallus Laminariae (Thallus Eckloniae) slurry temperature adjustment to 55 DEG C, add cellulase 40g, pectase 60g and compound protease 30g, enzymolysis
4h, obtains Thallus Laminariae (Thallus Eckloniae) enzymolysis solution；Wherein compound protein hydrolytic enzyme can be commercial Protamex compound protease；
By Chinese green food slurry temperature adjustment to 45 DEG C, adjusting pH value is 9.5, adds alkaline protease 140g, after enzymolysis 2h,
Adjusting pH value is 7, adds flavor protease 200g, digests 2h, obtain Chinese green food enzymolysis solution；
Apostichopus enzymolysis liquid obtained by step (b), Rhopilema esculenta enzymolysis solution, Thallus Laminariae (Thallus Eckloniae) enzymolysis solution, Chinese green food enzymolysis solution are divided by c, enzyme denaturing
It is not heated to Solutions Center temperature and is 98 DEG C, keep 10min, carry out destroy the enzyme treatment, be subsequently cooled to 4 DEG C, stand overnight；
Enzyme denaturing that step (c) is obtained by d, centrifugation, cooling, the Apostichopus enzymolysis liquid standing, Rhopilema esculenta enzymolysis solution, Thallus Laminariae (Thallus Eckloniae) enzymolysis solution, in
State's Acetess enzymolysis solution is respectively adopted tube centrifuge, controls rotating speed 2500 rpm, centrifugation removes insoluble matter, and collects supernatant；
E, membrane filtration：Stichopus japonicuss that step (d) is obtained, Rhopilema esculenta, Thallus Laminariae (Thallus Eckloniae), Chinese green food supernatant pass through tubular type membrane filtration respectively
Removing macromole, the organic membrane retention classification that its periosteum filtered solution is 3000Dalton by pore size more respectively, collect
Below 3000Dalton trapped fluid；The trapped fluid of its below 3000Dalton passes through nanofiltration concentrating and desalinating, obtains nanofiltration retentate fluid；
F, drying：Stichopus japonicuss that step (e) is obtained, Rhopilema esculenta, Thallus Laminariae (Thallus Eckloniae), Chinese green food nanofiltration retentate fluid are spray-dried, and compound, and pulverize
Cheng Fen, obtains composite blood pressure lowering peptide.
Embodiment 3
A kind of method producing composite blood pressure lowering peptide using marine organisms, its concrete technology step is as follows：
It is raw material that a, the selection of raw material and process weigh Stichopus japonicuss 8kg, Rhopilema esculenta 60kg, Thallus Laminariae (Thallus Eckloniae) 22kg and Chinese green food 10kg, respectively
Put into immersion 24h in cold water, desalination, clean, pull out and drain away the water, carry out making beating process using beater respectively, afterwards
Add 30 times pure water adopt colloid mill by material micronization processes, obtain Stichopus japonicuss slurry, Rhopilema esculenta slurry, Thallus Laminariae (Thallus Eckloniae) slurry and
Chinese green food slurry；
B, enzymolysis are by the Stichopus japonicuss slurry obtained by step (a), Rhopilema esculenta slurry, Thallus Laminariae (Thallus Eckloniae) slurry and Chinese green food slurry
It is heated to Solutions Center temperature and is 100 DEG C, keep 10min, go out except microorganism；Then enzymolysis processing, wherein each slurry are carried out
Enzymolysis condition be：
By Stichopus japonicuss slurry temperature adjustment to 52 DEG C of addition compound protease 24g, after enzymolysis 1.5h, add flavor protease 70g,
Enzymolysis 2h, obtains Apostichopus enzymolysis liquid；Wherein compound protein hydrolytic enzyme can be commercial Protamex compound protease；
By Rhopilema esculenta slurry temperature adjustment to 40 DEG C, adjust pH value 3.5, add pepsin 120g, after enzymolysis 3.5h, adjust temperature
Spend for 48 DEG C, pH value is 5.2, adds papain 480g, digests 1h, obtains Rhopilema esculenta enzymolysis solution；
By Thallus Laminariae (Thallus Eckloniae) slurry temperature adjustment to 45 DEG C, add cellulase 132g, pectase 44g and compound protease 110g, enzyme
Solution 3.5h, obtains Thallus Laminariae (Thallus Eckloniae) enzymolysis solution；Wherein compound protein hydrolytic enzyme can be commercial Protamex compound protease；
By Chinese green food slurry temperature adjustment to 55 DEG C, adjusting pH value is 9.5, adds alkaline protease 30g, after enzymolysis 1h,
Adjusting pH value is 7, adds flavor protease 20g, digests 1h, obtain Chinese green food enzymolysis solution；
Apostichopus enzymolysis liquid obtained by step (b), Rhopilema esculenta enzymolysis solution, Thallus Laminariae (Thallus Eckloniae) enzymolysis solution, Chinese green food enzymolysis solution are divided by c, enzyme denaturing
It is not heated to Solutions Center temperature and is 100 DEG C, keep 10min, carry out destroy the enzyme treatment, be subsequently cooled to 4 DEG C, stand overnight；
Enzyme denaturing that step (c) is obtained by d, centrifugation, cooling, the Apostichopus enzymolysis liquid standing, Rhopilema esculenta enzymolysis solution, Thallus Laminariae (Thallus Eckloniae) enzymolysis solution, in
State's Acetess enzymolysis solution is respectively adopted link-suspended basket centrifuge, controls rotating speed 3000 rpm, centrifugation removes insoluble matter, and collects supernatant
Liquid；
E, membrane filtration：Stichopus japonicuss that step (d) is obtained, Rhopilema esculenta, Thallus Laminariae (Thallus Eckloniae), Chinese green food supernatant pass through tubular type membrane filtration respectively
Removing macromole, the organic membrane retention classification that its periosteum filtered solution is 3000Dalton by pore size more respectively, collect
Below 3000Dalton trapped fluid；The trapped fluid of its below 3000Dalton passes through nanofiltration concentrating and desalinating, obtains nanofiltration retentate fluid；
F, drying：Stichopus japonicuss that step (e) is obtained, Rhopilema esculenta, Thallus Laminariae (Thallus Eckloniae), Chinese green food nanofiltration retentate fluid are spray-dried, and compound, and pulverize
Cheng Fen, obtains composite blood pressure lowering peptide.
Claims (2)
1. a kind of using marine organisms produce composite blood pressure lowering peptide method it is characterised in that：It is to choose Stichopus japonicuss, Rhopilema esculenta, sea
Band and Chinese green food are raw material, weigh by weight percentage：Stichopus japonicuss 5～10%, Rhopilema esculenta 50～65%, Thallus Laminariae (Thallus Eckloniae) 10～25% and China
Acetess 10～20% carry out desalination, sofening treatment respectively；Add 10～30 times of pure water to cross colloid more respectively after beating to grind
Pulp thing；Slurry heating is gone out except microorganism；Carry out enzymolysis processing after cooling, heat enzyme denaturing；After cooling, centrifugation removal is insoluble
Thing, takes supernatant to pass through tubular membrane filtering and removing macromole；Filtered solution is the organic of 3000Dalton by trapped molecular weight
Film is classified, and collects the trapped fluid of below 3000Dalton；Trapped fluid nanofiltration concentrating and desalinating, is dried, and compounds, obtains compound blood pressure lowering
Peptide.
2. a kind of using marine organisms produce composite blood pressure lowering peptide method it is characterised in that：Its concrete technology step is as follows：
It is raw material that a, the selection of raw material choose Stichopus japonicuss, Rhopilema esculenta, Thallus Laminariae (Thallus Eckloniae) and Chinese green food with process, weighs by weight percentage：Sea
Ginseng 5～10%, Rhopilema esculenta 50～65%, Thallus Laminariae (Thallus Eckloniae) 10～25% and Chinese green food 10～20% are respectively put into immersion more than 24h in cold water, go
Except salinity, clean, pull out and drain away the water, carry out making beating process using beater respectively, add 10～30 times of pure water afterwards
Using colloid mill by material micronization processes, obtain Stichopus japonicuss slurry, Rhopilema esculenta slurry, Thallus Laminariae (Thallus Eckloniae) slurry and Chinese green food slurry；
B, enzymolysis are by the Stichopus japonicuss slurry obtained by step (a), Rhopilema esculenta slurry, Thallus Laminariae (Thallus Eckloniae) slurry and Chinese green food slurry
It is heated to Solutions Center temperature and is 95～100 DEG C, keep 10min, go out except microorganism；Then carry out enzymolysis processing, wherein respectively starch
Shape thing enzymolysis condition be：
By Stichopus japonicuss slurry temperature adjustment to 50～55 DEG C of addition compound proteases, its consumption is 3～the 10 ‰ of Stichopus japonicuss weight, enzyme
After solution 1～2h, add flavor protease, its consumption is 2～the 10 ‰ of Stichopus japonicuss weight, digests 2～3h, obtains Apostichopus enzymolysis liquid；
By Rhopilema esculenta slurry temperature adjustment to 35～40 DEG C, adjust pH value 3～3.5, add pepsin, its consumption is Rhopilema esculenta weight
2～the 5 ‰ of amount, after enzymolysis 2～5h, adjust temperature and are 45～50 DEG C, pH value is 5～5.5, adds papain, its consumption
For Rhopilema esculenta weight 4～8 ‰, digest 1～3h, obtain Rhopilema esculenta enzymolysis solution；
By Thallus Laminariae (Thallus Eckloniae) slurry temperature adjustment to 45～55 DEG C, add cellulase, pectase and compound protease, digest 3～4h,
Obtain Thallus Laminariae (Thallus Eckloniae) enzymolysis solution；Wherein, the consumption of described cellulase be 4～the 8 ‰ of Thallus Laminariae (Thallus Eckloniae) weight, the consumption of pectase be that Thallus Laminariae (Thallus Eckloniae) weighs
2～the 6 ‰ of amount, the consumption of compound protease is 3～the 10 ‰ of Thallus Laminariae (Thallus Eckloniae) weight；
By Chinese green food slurry temperature adjustment to 45～55 DEG C, adjusting pH value is 9.5～10, adds alkaline protease, its use
Measure for Chinese green food weight 3～7 ‰, enzymolysis 1～2h after, adjust pH value be 6.5～7, add flavor protease, its consumption
For Chinese green food weight 2～10 ‰, digest 1～2h, obtain Chinese green food enzymolysis solution；
Apostichopus enzymolysis liquid obtained by step (b), Rhopilema esculenta enzymolysis solution, Thallus Laminariae (Thallus Eckloniae) enzymolysis solution, Chinese green food enzymolysis solution are divided by c, enzyme denaturing
It is not heated to Solutions Center temperature and is 95～100 DEG C, keep 10min, carry out destroy the enzyme treatment, be subsequently cooled to 4 DEG C, stood
Night；
Enzyme denaturing that step (c) is obtained by d, centrifugation, cooling, the Apostichopus enzymolysis liquid standing, Rhopilema esculenta enzymolysis solution, Thallus Laminariae (Thallus Eckloniae) enzymolysis solution, in
State's Acetess enzymolysis solution is respectively adopted centrifuge and removes insoluble matter, and collects supernatant；
E, membrane filtration：Stichopus japonicuss that step (d) is obtained, Rhopilema esculenta, Thallus Laminariae (Thallus Eckloniae), Chinese green food supernatant pass through tubular type membrane filtration respectively
Removing macromole, the organic membrane retention classification that its periosteum filtered solution is 3000Dalton by pore size more respectively, collect
Below 3000Dalton trapped fluid；The trapped fluid of its below 3000Dalton passes through nanofiltration concentrating and desalinating, obtains nanofiltration retentate fluid；
F, drying：Stichopus japonicuss that step (e) is obtained, Rhopilema esculenta, Thallus Laminariae (Thallus Eckloniae), Chinese green food nanofiltration retentate fluid are dried, and compound, powder processed, obtain
Composite blood pressure lowering peptide.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107083413A (en) * | 2017-03-16 | 2017-08-22 | 华侨大学 | A kind of method that sea cucumber internal organ prepare sea cucumber peptide and sea cucumber polysaccharide |
| CN109722462A (en) * | 2019-03-15 | 2019-05-07 | 宁波海洋研究院 | A kind of jellyfish reducing blood lipid peptide and preparation method thereof |
| CN114921516A (en) * | 2022-05-26 | 2022-08-19 | 上海海洋大学 | Macrobrachium nipponense immunoactive peptide and preparation method and application thereof |
| CN117886888A (en) * | 2024-03-13 | 2024-04-16 | 中国农业大学 | Sea cucumber peptide with uric acid reducing effect and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102409072A (en) * | 2011-11-29 | 2012-04-11 | 山东好当家海洋发展股份有限公司 | Method for preparing composite blood pressure lowering peptide by using marine organism |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102409072A (en) * | 2011-11-29 | 2012-04-11 | 山东好当家海洋发展股份有限公司 | Method for preparing composite blood pressure lowering peptide by using marine organism |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107083413A (en) * | 2017-03-16 | 2017-08-22 | 华侨大学 | A kind of method that sea cucumber internal organ prepare sea cucumber peptide and sea cucumber polysaccharide |
| CN109722462A (en) * | 2019-03-15 | 2019-05-07 | 宁波海洋研究院 | A kind of jellyfish reducing blood lipid peptide and preparation method thereof |
| CN109722462B (en) * | 2019-03-15 | 2022-03-04 | 宁波海洋研究院 | Jellyfish blood fat reducing peptide and preparation method thereof |
| CN114921516A (en) * | 2022-05-26 | 2022-08-19 | 上海海洋大学 | Macrobrachium nipponense immunoactive peptide and preparation method and application thereof |
| CN114921516B (en) * | 2022-05-26 | 2024-03-15 | 上海海洋大学 | Chinese lobster immunocompetent peptide, and preparation method and application thereof |
| CN117886888A (en) * | 2024-03-13 | 2024-04-16 | 中国农业大学 | Sea cucumber peptide with uric acid reducing effect and application thereof |
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