CN102409072A - Method for preparing composite blood pressure lowering peptide by using marine organism - Google Patents
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Abstract
The invention relates to a method for preparing composite blood pressure lowering peptide by using a marine organism. The method comprises the following steps of: weighing the following raw materials in percentage by weight: 5-10 percent of sea cucumber, 50-65 percent of jellyfish, 10-25 percent of kelp and 10-20 percent of acetes chinensis; desalting and softening respectively; pulping respectively and then adding water to grind into paste; heating to kill off microorganisms; cooling, then performing enzymatic treatment and deactivating enzyme; cooling and then centrifuging, and filtering supernatant through a tubular membrane to remove macromolecules; and grading filtrate through an organic membrane with molecular weight cutoff of 3,000 Dalton, collecting cutoff liquid of below 3,000 Dalton, and nanofiltering, concentrating, desalting, drying and compounding to obtain the composite blood pressure lowering peptide. In the method, an optimal enzymolysis technical scheme is determined; and in tubular filtration, ultrafiltration, nanofiltration separation extraction technologies, the extraction efficiency is high, and the resultant composite blood pressure lowering peptide has an obvious pressure lowering effect. The method has the advantages of scientific material selection, rational process, easiness and convenience in operation and easiness in implementation of industrial production.
Description
Technical field
The present invention relates to a kind of preparation of peptide, especially a kind of method of utilizing sea life to produce compound blood pressure lowering peptide.
Background technology
Blood pressure lowering peptide is called angiotensin-converting enzyme (Angiotensin Converting Enzyme, EC3.4.15.1 are called for short ACE) inhibiting peptide again, is isolated one type of peptide with the effect that significantly brings high blood pressure down from food proteins, is generally small-molecular peptides.In numerous depressor substances, blood pressure lowering peptide becomes the focus of bioactive peptide research because of its buck functionality that has no side effect.Isolating blood pressure lowering peptide from natural food albumen, though antihypertensive effect is so strong not as synthetic drugs, it has safe, only the hyperpietic is played hypotensive effect, and the normotensive is not had hypotensive effect, does not have the step-down over-education phenomenon and takes place.The present casein food grade from MC, whey-protein etc.; Chicken in the animal/vegetable protein, gelatin, pork, egg, buckwheat, corn, cabbage, garlic etc.; Isolate ACE inhibitor in the numerous food products such as the sardines in the fish shellfish algae protein, tuna, Ricefield eel head, oyster, wakame; Domestic investigator also from the traditional food Ji Me Lao Jiu of China, finds ace inhibitory peptide in the soy cheese.
Modern medicine study proves that sea cucumber is rich in more than 50 kind of nutritive ingredients to the human body beneficial such as polysaccharide, holothurin, sea cucumber saponin, L-glutamic acid, glycocoll, l-arginine, selenium, zinc, iron, iodine, calcium, superoxide-dismutase (SOD), CHS.Through measuring, the sea cucumber range of hydrolysed peptides also has certain ACE and suppresses active, is the potential material for lowering blood pressure.In addition, the jellyfish kind is many, and quantity is big, and it is wide to distribute in China, is the important component part of marine zooplankton group.The jellyfish body contains inorganic salt such as higher protein and calcium, phosphorus, iodine, iron, have the laudatory title of " aquatic products rarity ", and traditional Chinese medicine thinks that jellyfish has vasodilation, hypotensive effect.In addition, Chinese shrimp is the peculiar kind of China, and China is coastal all to have distribution, maximum with the littoral output in the Bohai Sea especially.And containing branched-chain amino acid and the die aromatischen Aminosaeuren relevant in the amino acid in the shrimp with the blood pressure lowering peptide structure, Chinese shrimp is the good raw material that depressor substance extracts.In addition, sea-tangle contains phycocolloid acid, laminaran, polyoses such as Polygalactan, laminine, L-glutamic acid, aspartic acid, amino acid such as proline(Pro), VITMAIN B1, B2, C, P and Serlabo, inorganic salt such as iodine, potassium, calcium.The traditional Chinese medical science thinks that sea-tangle has effects such as softening and resolving hard mass, eliminating sputum to relieve asthma, current Li Shui, the lipoid and reducing blood pressure of dispelling.Modern medicine study shows, the laminine and the N.F,USP MANNITOL that extract in the sea-tangle have hypotensive effect.
At present, domestic separation and purification, structure evaluation and the activity rating aspect that mainly concentrates on peptide about the research of blood pressure lowering peptide.How confirming the enzymatic hydrolysis condition of the best, optimization and blood pressure lowering peptide is improved separation and Extraction efficient is a technical problem that needs to be resolved hurrily.Simultaneously, at present, also not seeing has the research report that utilizes multiple sea life to produce compound blood pressure lowering peptide.
Summary of the invention
In order to overcome the deficiency of prior art, the present invention provides a kind of method of utilizing sea life to produce compound blood pressure lowering peptide.The raw material of this method is chosen science, technology is reasonable, easy and simple to handle, workable, separation and Extraction efficient high, is prone to realize suitability for industrialized production.According to this method and produce the compound blood pressure lowering peptide of gained, have good step-down effect.
The technical solution adopted for the present invention to solve the technical problems is: a kind of method of utilizing sea life to produce compound blood pressure lowering peptide; It is that to choose sea cucumber, jellyfish, sea-tangle and Chinese shrimp be raw material, takes by weighing by weight percentage: sea cucumber 5～10%, jellyfish 50～65%, sea-tangle 10～25% and Chinese shrimp 10～20% carry out desalination, the softening processing respectively; Cross colloidal mill through the pure water of 10～30 times of making beating back addings respectively again and process soup compound; The soup compound heating is gone out except that mikrobe; Carry out enzymolysis processing after the cooling, heat the enzyme that goes out; The centrifugal removal insolubles in cooling back is got supernatant through tubular membrane filtering and removing macromole; Filtered solution is the organic membrane classification of 3000Dalton through trapped molecular weight, collects the trapped fluid below the 3000Dalton; Trapped fluid nanofiltration concentrating and desalinating, drying, composite, get compound blood pressure lowering peptide.
It is raw material that the present invention chooses sea cucumber, jellyfish, sea-tangle and Chinese shrimp, and respectively through desalination, the softening processing, respectively through adding the water mill slurry, heating is gone out except that mikrobe again with each raw material; Cooling back enzymolysis, enzyme goes out; Centrifugal, get supernatant more respectively through pipe filter, ultrafiltration, nanofiltration, drying, composite, get compound blood pressure lowering peptide.The present invention is that sea cucumber, jellyfish, sea-tangle and the Chinese shrimp that scientific and reasonable choosing has buck functionality is raw material; To have ACE through enzymolysis, pipe filter, ultrafiltration, nanofiltration and suppress active small-molecular peptides and separate, and preserve its intact structure and effective biological activity.Experiment shows, adopt the isolated blood pressure lowering peptide composition of this technology composite after, through the original hypertensive rat experiment, can reduce the rat diastolic pressure significantly.Confirmed the zymolysis technique scheme of optimum optimization in this method, the pipe filter of being adopted, ultrafiltration, nanofiltration separation extractive technique make the efficient of its extraction high.The method technology of utilizing sea life to produce compound blood pressure lowering peptide of the present invention is reasonable, easy and simple to handle, workable, is prone to realize suitability for industrialized production.
Embodiment
A kind of method of utilizing sea life to produce compound blood pressure lowering peptide, its concrete process step is following:
It is raw material that sea cucumber, jellyfish, sea-tangle and Chinese shrimp are chosen in choose and the processing of a, raw material; Take by weighing by weight percentage: sea cucumber 5～10%, jellyfish 50～65%, sea-tangle 10～25% and Chinese shrimp 10～20% are put into cold water respectively and soak more than the 24h; Remove salinity; Clean, pull out and drain away the water, use the hollander processing of pulling an oar respectively; The pure water that adds 10～30 times afterwards adopts colloidal mill with the material thinning processing, gets sea cucumber soup compound, jellyfish soup compound, sea-tangle soup compound and Chinese shrimp soup compound;
It is 95～100 ℃ that b, enzymolysis are heated to the solution core temperature with the resulting sea cucumber soup compound of step (a), jellyfish soup compound, sea-tangle soup compound and Chinese shrimp soup compound, keeps 10min, goes out except that mikrobe; Carry out enzymolysis processing then, wherein the condition of each soup compound enzymolysis is:
With sea cucumber soup compound temperature regulation to 50～55 ℃ of adding compound proteases, its consumption is 3 of sea cucumber weight～10 ‰, behind enzymolysis 1～2h, adds flavor protease, and its consumption is 2 of sea cucumber weight～10 ‰, and enzymolysis 2～3h gets sea cucumber hydrolysate;
With jellyfish soup compound temperature regulation to 35～40 ℃, regulate pH value 3～3.5, the adding stomach en-; Its consumption is 2 of jellyfish weight～5 ‰, and behind enzymolysis 2～5h, attemperation is 45～50 ℃; The pH value is 5～5.5, adds papoid, and its consumption is 4 of jellyfish weight～8 ‰; Enzymolysis 1～3h gets the jellyfish enzymolysis solution;
With sea-tangle soup compound temperature regulation to 45～55 ℃, add cellulase, polygalacturonase and compound protease, enzymolysis 3～4h, the sea-tangle enzymolysis solution; Wherein, the consumption of described cellulase is that the consumption of 4 of sea-tangle weight～8 ‰, polygalacturonase is that the consumption of 2 of sea-tangle weight～6 ‰, compound protease is 3 of sea-tangle weight～10 ‰;
With Chinese shrimp soup compound temperature regulation to 45～55 ℃, regulating pH value is 9.5～10, the adding Sumizyme MP; Its consumption be Chinese shrimp weight 3～7 ‰, behind enzymolysis 1～2h, regulating the pH value is 6.5～7; Add flavor protease; Its consumption is 2 of Chinese shrimp weight～10 ‰, and enzymolysis 1～2h gets Chinese shrimp enzymolysis solution;
It is 95～100 ℃ that c, the enzyme that goes out are heated to the solution core temperature respectively with the resulting sea cucumber hydrolysate of step (b), jellyfish enzymolysis solution, sea-tangle enzymolysis solution, Chinese shrimp enzymolysis solution, keeps 10min, and the enzyme that goes out is handled, and is cooled to 4 ℃ then, hold over night;
D, the centrifugal enzyme that goes out, the sea cucumber hydrolysate of cooling off, leaving standstill, jellyfish enzymolysis solution, sea-tangle enzymolysis solution, the Chinese shrimp enzymolysis solution that step (c) is obtained adopt the centrifugal removal insolubles of whizzer respectively, and collect supernatant;
E, membrane filtration: sea cucumber, jellyfish, sea-tangle, Chinese shrimp supernatant that step (d) is obtained pass through tubular membrane filtering and removing macromole respectively; Its periosteum filtered solution is that the organic membrane of 3000Dalton is held back classification through pore size respectively again, collects the following trapped fluid of 3000Dalton; Trapped fluid below its 3000Dalton gets the nanofiltration trapped fluid through the nanofiltration concentrating and desalinating;
F, drying: sea cucumber, jellyfish, sea-tangle, Chinese shrimp nanofiltration trapped fluid that step (e) obtains is dry, composite, and powder process gets compound blood pressure lowering peptide.
further specify the present invention below in conjunction with embodiment.
Embodiment 1
A kind of method of utilizing sea life to produce compound blood pressure lowering peptide, its concrete process step is following:
It is raw material that choose and the processing of a, raw material takes by weighing sea cucumber 10kg, jellyfish 50kg, sea-tangle 25kg and Chinese shrimp 15kg; Put into cold water respectively and soak 36h, remove salinity, clean; Pull out and drain away the water; Use the hollander processing of pulling an oar respectively, the pure water that adds 20 times afterwards adopts colloidal mill with the material thinning processing, sea cucumber soup compound, jellyfish soup compound, sea-tangle soup compound and Chinese shrimp soup compound;
It is 100 ℃ that b, enzymolysis are heated to the solution core temperature with the resulting sea cucumber soup compound of step (a), jellyfish soup compound, sea-tangle soup compound and Chinese shrimp soup compound, keeps 10min, goes out except that mikrobe; Carry out enzymolysis processing then, wherein the condition of each soup compound enzymolysis is:
With sea cucumber soup compound temperature regulation to 55 ℃ adding compound protease 90g, behind the enzymolysis 1h, add flavor protease 20g, enzymolysis 3h gets sea cucumber hydrolysate; Wherein the conjugated protein lytic enzyme can be commercial commercial enzyme, like the Protamex compound protease, also can prepare voluntarily, contains papoid and glycase in the conjugated protein lytic enzyme of preparation.What present embodiment adopted is to prepare the conjugated protein lytic enzyme voluntarily;
With jellyfish soup compound temperature regulation to 37 ℃, regulate pH value 3, add stomach en-250g, behind the enzymolysis 2h, attemperation is 50 ℃, pH value is 5, adding papoid 200g, enzymolysis 2h must the jellyfish enzymolysis solution;
With sea-tangle soup compound temperature regulation to 50 ℃, add cellulase 200g, polygalacturonase 100g and compound protease 250g, enzymolysis 3h, the sea-tangle enzymolysis solution; What wherein the conjugated protein lytic enzyme adopted is to prepare the conjugated protein lytic enzyme voluntarily;
With Chinese shrimp soup compound temperature regulation to 50 ℃, regulating pH value is 10, adds Sumizyme MP 75g, and behind the enzymolysis 1.5h, regulating pH value is 6.5, adding flavor protease 150g, and enzymolysis 1.5h gets Chinese shrimp enzymolysis solution;
It is 100 ℃ that c, the enzyme that goes out are heated to the solution core temperature respectively with the resulting sea cucumber hydrolysate of step (b), jellyfish enzymolysis solution, sea-tangle enzymolysis solution, Chinese shrimp enzymolysis solution, keeps 10min, and the enzyme that goes out is handled, and is cooled to 4 ℃ then, hold over night;
D, the centrifugal enzyme that goes out, the sea cucumber hydrolysate of cooling off, leaving standstill, jellyfish enzymolysis solution, sea-tangle enzymolysis solution, the Chinese shrimp enzymolysis solution that step (c) is obtained adopt link-suspended basket centrifuge respectively, control rotating speed 2000 rpm, and centrifugal removal insolubles, and collect supernatant;
E, membrane filtration: sea cucumber, jellyfish, sea-tangle, Chinese shrimp supernatant that step (d) is obtained pass through tubular membrane filtering and removing macromole respectively; Its periosteum filtered solution is that the organic membrane of 3000Dalton is held back classification through pore size respectively again, collects the following trapped fluid of 3000Dalton; Trapped fluid below its 3000Dalton gets the nanofiltration trapped fluid through the nanofiltration concentrating and desalinating;
F, drying: sea cucumber, jellyfish, sea-tangle, the Chinese shrimp nanofiltration trapped fluid spraying drying that step (e) obtains is composite, be ground into powder, get compound blood pressure lowering peptide.
Embodiment 2
A kind of method of utilizing sea life to produce compound blood pressure lowering peptide, its concrete process step is following:
It is raw material that choose and the processing of a, raw material takes by weighing sea cucumber 5kg, jellyfish 65kg, sea-tangle 10kg and Chinese shrimp 20kg; Put into cold water respectively and soak 48h, remove salinity, clean; Pull out and drain away the water; Use the hollander processing of pulling an oar respectively, the pure water that adds 10 times afterwards adopts colloidal mill with the material thinning processing, sea cucumber soup compound, jellyfish soup compound, sea-tangle soup compound and Chinese shrimp soup compound;
It is 95 ℃ that b, enzymolysis are heated to the solution core temperature with the resulting sea cucumber soup compound of step (a), jellyfish soup compound, sea-tangle soup compound and Chinese shrimp soup compound, keeps 10min, goes out except that mikrobe; Carry out enzymolysis processing then, wherein the condition of each soup compound enzymolysis is:
With sea cucumber soup compound temperature regulation to 50 ℃ adding compound protease 25g, behind the enzymolysis 2h, add flavor protease 25g, enzymolysis 2.5h gets sea cucumber hydrolysate; Wherein the conjugated protein lytic enzyme can be commercial Protamex compound protease;
With jellyfish soup compound temperature regulation to 35 ℃, regulate pH value 3.3, add stomach en-195g, behind the enzymolysis 3h, attemperation is 45 ℃, pH value is 5.5, adding papoid 325g, enzymolysis 3h must the jellyfish enzymolysis solution;
With sea-tangle soup compound temperature regulation to 55 ℃, add cellulase 40g, polygalacturonase 60g and compound protease 30g, enzymolysis 4h, the sea-tangle enzymolysis solution; Wherein the conjugated protein lytic enzyme can be commercial Protamex compound protease;
With Chinese shrimp soup compound temperature regulation to 45 ℃, regulating pH value is 9.5, adds Sumizyme MP 140g, and behind the enzymolysis 2h, regulating pH value is 7, adding flavor protease 200g, and enzymolysis 2h gets Chinese shrimp enzymolysis solution;
It is 98 ℃ that c, the enzyme that goes out are heated to the solution core temperature respectively with the resulting sea cucumber hydrolysate of step (b), jellyfish enzymolysis solution, sea-tangle enzymolysis solution, Chinese shrimp enzymolysis solution, keeps 10min, and the enzyme that goes out is handled, and is cooled to 4 ℃ then, hold over night;
D, the centrifugal enzyme that goes out, the sea cucumber hydrolysate of cooling off, leaving standstill, jellyfish enzymolysis solution, sea-tangle enzymolysis solution, the Chinese shrimp enzymolysis solution that step (c) is obtained adopt tubular-bowl centrifuge respectively, control rotating speed 2500 rpm, and centrifugal removal insolubles, and collect supernatant;
E, membrane filtration: sea cucumber, jellyfish, sea-tangle, Chinese shrimp supernatant that step (d) is obtained pass through tubular membrane filtering and removing macromole respectively; Its periosteum filtered solution is that the organic membrane of 3000Dalton is held back classification through pore size respectively again, collects the following trapped fluid of 3000Dalton; Trapped fluid below its 3000Dalton gets the nanofiltration trapped fluid through the nanofiltration concentrating and desalinating;
F, drying: sea cucumber, jellyfish, sea-tangle, the Chinese shrimp nanofiltration trapped fluid spraying drying that step (e) obtains is composite, be ground into powder, get compound blood pressure lowering peptide.
Embodiment 3
A kind of method of utilizing sea life to produce compound blood pressure lowering peptide, its concrete process step is following:
It is raw material that choose and the processing of a, raw material takes by weighing sea cucumber 8kg, jellyfish 60kg, sea-tangle 22kg and Chinese shrimp 10kg; Put into cold water respectively and soak 24h, remove salinity, clean; Pull out and drain away the water; Use the hollander processing of pulling an oar respectively, the pure water that adds 30 times afterwards adopts colloidal mill with the material thinning processing, sea cucumber soup compound, jellyfish soup compound, sea-tangle soup compound and Chinese shrimp soup compound;
It is 100 ℃ that b, enzymolysis are heated to the solution core temperature with the resulting sea cucumber soup compound of step (a), jellyfish soup compound, sea-tangle soup compound and Chinese shrimp soup compound, keeps 10min, goes out except that mikrobe; Carry out enzymolysis processing then, wherein the condition of each soup compound enzymolysis is:
With sea cucumber soup compound temperature regulation to 52 ℃ adding compound protease 24g, behind the enzymolysis 1.5h, add flavor protease 70g, enzymolysis 2h gets sea cucumber hydrolysate; Wherein the conjugated protein lytic enzyme can be commercial Protamex compound protease;
With jellyfish soup compound temperature regulation to 40 ℃, regulate pH value 3.5, add stomach en-120g, behind the enzymolysis 3.5h, attemperation is 48 ℃, pH value is 5.2, adding papoid 480g, enzymolysis 1h must the jellyfish enzymolysis solution;
With sea-tangle soup compound temperature regulation to 45 ℃, add cellulase 132g, polygalacturonase 44g and compound protease 110g, enzymolysis 3.5h, the sea-tangle enzymolysis solution; Wherein the conjugated protein lytic enzyme can be commercial Protamex compound protease;
With Chinese shrimp soup compound temperature regulation to 55 ℃, regulating pH value is 9.5, adds Sumizyme MP 30g, and behind the enzymolysis 1h, regulating pH value is 7, adding flavor protease 20g, and enzymolysis 1h gets Chinese shrimp enzymolysis solution;
It is 100 ℃ that c, the enzyme that goes out are heated to the solution core temperature respectively with the resulting sea cucumber hydrolysate of step (b), jellyfish enzymolysis solution, sea-tangle enzymolysis solution, Chinese shrimp enzymolysis solution, keeps 10min, and the enzyme that goes out is handled, and is cooled to 4 ℃ then, hold over night;
D, the centrifugal enzyme that goes out, the sea cucumber hydrolysate of cooling off, leaving standstill, jellyfish enzymolysis solution, sea-tangle enzymolysis solution, the Chinese shrimp enzymolysis solution that step (c) is obtained adopt link-suspended basket centrifuge respectively, control rotating speed 3000 rpm, and centrifugal removal insolubles, and collect supernatant;
E, membrane filtration: sea cucumber, jellyfish, sea-tangle, Chinese shrimp supernatant that step (d) is obtained pass through tubular membrane filtering and removing macromole respectively; Its periosteum filtered solution is that the organic membrane of 3000Dalton is held back classification through pore size respectively again, collects the following trapped fluid of 3000Dalton; Trapped fluid below its 3000Dalton gets the nanofiltration trapped fluid through the nanofiltration concentrating and desalinating;
F, drying: sea cucumber, jellyfish, sea-tangle, the Chinese shrimp nanofiltration trapped fluid spraying drying that step (e) obtains is composite, be ground into powder, get compound blood pressure lowering peptide.
Further specify antihypertensive effect of the present invention with experimentation on animals below
.
This experimentation on animals is that 55 SHR are divided into 5 groups at random, blank group, sample low dose group, middle dose groups and high dose group and positive controls, 11 every group.Blank group: give 0.9% saline water filling stomach.Low dose group: give sample aqueous solution and irritate stomach with 200 mg/kgd dosage; Middle dose groups: give sample and irritate stomach with 400 mg/kgd dosage; Sample high dose group: give sample aqueous solution and irritate stomach with 800 mg/kgd dosage; Positive controls: give captopril 0.54 mg/kgd dosage and irritate stomach.5 weeks of successive administration.Measure the caudal artery systolic pressure weekly once, data are seen attached list.
The variation of subordinate list SHR caudal artery systolic pressure (Mean ± SD, n=11)
Subordinate list is continuous
Annotate: compare with the blank group
*P<0.05,
*P<0.01
After 5 weeks of administration; The basic, normal, high dose groups of sample is compared with the blank group; The caudal artery systolic pressure of SHR all has obvious decline, and along with the prolongation of the time of continuing medication, SHR caudal artery systolic pressure downward trend is slowed down and tended towards stability gradually; Its drug effect presents good dose-dependent relationship simultaneously, and the antihypertensive effect of high dose group is similar with the positive control captopril.
Claims (2)
1. method of utilizing sea life to produce compound blood pressure lowering peptide; It is characterized in that: it is that to choose sea cucumber, jellyfish, sea-tangle and Chinese shrimp be raw material, takes by weighing by weight percentage: sea cucumber 5～10%, jellyfish 50～65%, sea-tangle 10～25% and Chinese shrimp 10～20% carry out desalination, the softening processing respectively; Cross colloidal mill through the pure water of 10～30 times of making beating back addings respectively again and process soup compound; The soup compound heating is gone out except that mikrobe; Carry out enzymolysis processing after the cooling, heat the enzyme that goes out; The centrifugal removal insolubles in cooling back is got supernatant through tubular membrane filtering and removing macromole; Filtered solution is the organic membrane classification of 3000Dalton through trapped molecular weight, collects the trapped fluid below the 3000Dalton; Trapped fluid nanofiltration concentrating and desalinating, drying, composite, get compound blood pressure lowering peptide.
2. method of utilizing sea life to produce compound blood pressure lowering peptide, it is characterized in that: its concrete process step is following:
It is raw material that sea cucumber, jellyfish, sea-tangle and Chinese shrimp are chosen in choose and the processing of a, raw material; Take by weighing by weight percentage: sea cucumber 5～10%, jellyfish 50～65%, sea-tangle 10～25% and Chinese shrimp 10～20% are put into cold water respectively and soak more than the 24h; Remove salinity; Clean, pull out and drain away the water, use the hollander processing of pulling an oar respectively; The pure water that adds 10～30 times afterwards adopts colloidal mill with the material thinning processing, gets sea cucumber soup compound, jellyfish soup compound, sea-tangle soup compound and Chinese shrimp soup compound;
It is 95～100 ℃ that b, enzymolysis are heated to the solution core temperature with the resulting sea cucumber soup compound of step (a), jellyfish soup compound, sea-tangle soup compound and Chinese shrimp soup compound, keeps 10min, goes out except that mikrobe; Carry out enzymolysis processing then, wherein the condition of each soup compound enzymolysis is:
With sea cucumber soup compound temperature regulation to 50～55 ℃ of adding compound proteases, its consumption is 3 of sea cucumber weight～10 ‰, behind enzymolysis 1～2h, adds flavor protease, and its consumption is 2 of sea cucumber weight～10 ‰, and enzymolysis 2～3h gets sea cucumber hydrolysate;
With jellyfish soup compound temperature regulation to 35～40 ℃, regulate pH value 3～3.5, the adding stomach en-; Its consumption is 2 of jellyfish weight～5 ‰, and behind enzymolysis 2～5h, attemperation is 45～50 ℃; The pH value is 5～5.5, adds papoid, and its consumption is 4 of jellyfish weight～8 ‰; Enzymolysis 1～3h gets the jellyfish enzymolysis solution;
With sea-tangle soup compound temperature regulation to 45～55 ℃, add cellulase, polygalacturonase and compound protease, enzymolysis 3～4h, the sea-tangle enzymolysis solution; Wherein, the consumption of described cellulase is that the consumption of 4 of sea-tangle weight～8 ‰, polygalacturonase is that the consumption of 2 of sea-tangle weight～6 ‰, compound protease is 3 of sea-tangle weight～10 ‰;
With Chinese shrimp soup compound temperature regulation to 45～55 ℃, regulating pH value is 9.5～10, the adding Sumizyme MP; Its consumption be Chinese shrimp weight 3～7 ‰, behind enzymolysis 1～2h, regulating the pH value is 6.5～7; Add flavor protease; Its consumption is 2 of Chinese shrimp weight～10 ‰, and enzymolysis 1～2h gets Chinese shrimp enzymolysis solution;
It is 95～100 ℃ that c, the enzyme that goes out are heated to the solution core temperature respectively with the resulting sea cucumber hydrolysate of step (b), jellyfish enzymolysis solution, sea-tangle enzymolysis solution, Chinese shrimp enzymolysis solution, keeps 10min, and the enzyme that goes out is handled, and is cooled to 4 ℃ then, hold over night;
D, the centrifugal enzyme that goes out, the sea cucumber hydrolysate of cooling off, leaving standstill, jellyfish enzymolysis solution, sea-tangle enzymolysis solution, the Chinese shrimp enzymolysis solution that step (c) is obtained adopt the centrifugal removal insolubles of whizzer respectively, and collect supernatant;
E, membrane filtration: sea cucumber, jellyfish, sea-tangle, Chinese shrimp supernatant that step (d) is obtained pass through tubular membrane filtering and removing macromole respectively; Its periosteum filtered solution is that the organic membrane of 3000Dalton is held back classification through pore size respectively again, collects the following trapped fluid of 3000Dalton; Trapped fluid below its 3000Dalton gets the nanofiltration trapped fluid through the nanofiltration concentrating and desalinating;
F, drying: sea cucumber, jellyfish, sea-tangle, Chinese shrimp nanofiltration trapped fluid that step (e) obtains is dry, composite, and powder process gets compound blood pressure lowering peptide.
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| CN102754866A (en) * | 2012-07-26 | 2012-10-31 | 天津市春升清真食品有限公司 | Flavor kelp meal |
| CN103518941A (en) * | 2012-11-29 | 2014-01-22 | 中国科学院海洋研究所 | Method for preparing jellyfish gonad hydrolyzed protein |
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| CN104435732A (en) * | 2014-12-09 | 2015-03-25 | 山东好当家海洋发展股份有限公司 | Stichopus japonicus traditional Chinese medicine soft capsules capable of promoting wound healing and preparation method thereof |
| CN104888192A (en) * | 2015-04-30 | 2015-09-09 | 威海特伦斯生物工程有限公司 | Sea cucumber glucosamine preparation for increasing bone mineral density, and production method thereof |
| CN105146045B (en) * | 2015-09-22 | 2018-09-14 | 华南理工大学 | Sea-fish protein prepares the method for combining blood pressure lowering peptide with fresh water fish protein |
| CN105146045A (en) * | 2015-09-22 | 2015-12-16 | 华南理工大学 | Method for using ocean fish protein and freshwater fish protein for preparing combined antihypertensive peptides |
| CN106281900A (en) * | 2016-08-31 | 2017-01-04 | 辽宁双增生物工程有限公司 | A kind of Stichopus japonicus polygonatum liquor preparation method |
| CN106480147A (en) * | 2016-11-01 | 2017-03-08 | 福建农林大学 | A kind of method that red koji fermentation sea-tangle hydrolyzate prepares blood pressure lowering peptide |
| CN106538991A (en) * | 2016-11-01 | 2017-03-29 | 福建农林大学 | A kind of method that ultrasonic acidolysis pre-treat biological prepares kelp blood pressure product |
| CN106538955A (en) * | 2016-11-01 | 2017-03-29 | 福建农林大学 | A kind of blood pressure lowering restructuring Thallus Laminariae (Thallus Eckloniae) cold and fresh noodles bar and preparation method thereof |
| CN106434814A (en) * | 2016-11-27 | 2017-02-22 | 威海蓝印海洋生物科技有限公司 | Method for preparing compounded antihypertensive peptide through marine organisms |
| CN111153942A (en) * | 2019-07-09 | 2020-05-15 | 山东省科学院生物研究所 | Preparation method of ACE (angiotensin converting enzyme) inhibitory active compound from hawthorn flower |
| CN111153942B (en) * | 2019-07-09 | 2021-07-13 | 山东省科学院生物研究所 | Preparation method of ACE (angiotensin converting enzyme) inhibitory active compound from hawthorn flower |
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Effective date of registration: 20190513 Address after: 264300 No. 318 Taixiang Road, Rongcheng City, Shandong Province Patentee after: Shandong Homey Haisen Medicine Co., Ltd. Address before: 264305 Home-Owned Industrial Park, Hushan Town, Rongcheng City, Weihai City, Shandong Province Co-patentee before: Biologic Inst., Shandong Prov. Academy of Sciences Patentee before: Shandong Homey Aquatic Development Co., Ltd. |