CN106480147A - A kind of method that red koji fermentation sea-tangle hydrolyzate prepares blood pressure lowering peptide - Google Patents
A kind of method that red koji fermentation sea-tangle hydrolyzate prepares blood pressure lowering peptide Download PDFInfo
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- CN106480147A CN106480147A CN201610936934.5A CN201610936934A CN106480147A CN 106480147 A CN106480147 A CN 106480147A CN 201610936934 A CN201610936934 A CN 201610936934A CN 106480147 A CN106480147 A CN 106480147A
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Abstract
The present invention relates to a kind of method that red koji fermentation sea-tangle hydrolyzate prepares blood pressure lowering peptide, its be with kelp processing waste products degumming Kelp Powder as raw material, supernatant is taken after dissolving, ultrasound and centrifugation, add mixed enzyme to carry out bioconversion and sea-tangle hydrolyzate is obtained, and which is mixed and made into fermentation medium with certain component, after sterilizing, inoculation monascus specie is fermented, and obtains the kelp blood pressure peptides products with notable hypotensive activity.The technical scheme that the method is combined with red koji fermentation using enzymolysis, improves kelp blood pressure peptide purity and activity, while generating other hypotensive bioactive ingredients such as gamma aminobutyric acid.In the blood pressure lowering peptide product that the present invention is prepared, the content of biologically active peptide is 10.5 ~ 16.3 g/100g.Zoopery shows that high dose group kelp blood pressure peptides products reduce by 37.5 mmHg of primary rat blood pressure, with significant hypotensive activity.
Description
Technical field
The invention belongs to food biotechnology and functional health field, and in particular to a kind of red koji fermentation sea-tangle hydrolyzate system
The method of standby blood pressure lowering peptide.
Background technology
Hypertension is to perplex a kind of global disease now, and it can induce coronary heart disease, cerebral apoplexy and cardiorenal function and decline
Exhaust.The treatment of hypertension needs the quite a long time, and has certain toxic and side effect medicine main at present more, no
Suitable long-term taking.With the enhancing of people's health care consciousness and awareness of safety, in numerous blood-pressure drugs, with notable drop
The blood pressure lowering peptide of blood pressure activity and safety non-toxic is gradually used in prevention and treatment hypertension.
Blood pressure lowering peptide, also known as inhibiting peptide of tonin, is the class from separation and Extraction in natural food albumen
Small-molecular peptides.Compared with clinical drug for hypertension, although the antihypertensive effect of blood pressure lowering peptide is strong not as which, food-borne
Blood pressure lowering peptide is safe, and it only plays hypotensive effect to hyperpietic, to normotensive people nothing obvious antihypertensive effect, fits
Close patient's long-term taking.Therefore, it has become as a study hotspot in hypotensive field at present.
Contain the nutrient content such as polysaccharide, amino acid, vitamin and inorganic salts in sea-tangle.Modern scientific research finds, extra large
Band not only has the functions such as lowering blood pressure and blood fat, anti-oxidant, anti-mutation and strengthen immunity, and studies have found that sea-tangle
Contain a certain amount of blood pressure lowering peptide active component in hydrolysate, be therefore traditionally regarded as marine Chinese medicine.Sea-tangle is single
Extraction algin after be degumming sea-tangle, degumming sea-tangle is generally regarded as waste material and does not continue to processing and utilization.However, degumming is extra large
Band still maintains the original major part high-quality resource of sea-tangle, its discarded huge waste for causing sea-tangle resource in vivo.
Red yeast rice has medical value from ancient times, produces GABA, Lovastatin, monascorubin, gland in sweat
Glycosides, small molecule active group are graded lowering blood pressure and blood fat active component.But at present, the country is yet there are no using red koji fermentation sea-tangle water
Solution liquid prepares the research report of blood pressure lowering peptide.
Content of the invention
It is an object of the invention to provide one kind is with kelp processing waste products degumming Kelp Powder as raw material, using enzymolysis and red yeast rice
Fermentation combines, and rational technology is effective, the method that the good red koji fermentation sea-tangle hydrolyzate of antihypertensive effect prepares blood pressure lowering peptide.
In order to realize the object of the invention, adopt the following technical scheme that：
A kind of method that red koji fermentation sea-tangle hydrolyzate prepares blood pressure lowering peptide, comprises the following steps：
1）Pretreatment of raw material：Degumming Kelp Powder and concentration are that the hydrochloric acid of 1.0～2.0 mol/L compares 1 with weight:10～1:20 mix
Closing, fully dissolving, under ultrasonic assistant effect, temperature is 45～60 DEG C of 2～6 h of hydrolysis, 5～10 is centrifuged in 4000 r/min
Min, supernatant are sea-tangle pretreatment fluid；
2）Prepared by sea-tangle hydrolyzate：The pH for adjusting sea-tangle pretreatment fluid is 7.0～8.5, adds mixing in sea-tangle pretreatment fluid
Enzyme carries out bioconversion, and mixed enzyme addition is the 0.2～3% of sea-tangle pretreatment fluid weight, and conversion temperature is 35～60 DEG C, turns
The change time is 2-8 h, obtains sea-tangle hydrolyzate after the completion of bioconversion；
3）Prepared by red koji fermentation culture medium：In sea-tangle hydrolyzate add sea-tangle hydrolyzate weight 1～2% soluble starch, 1
～2% peptone, 0.05～0.2% MgSO4·7H2O, 0.1～0.3% NaNO3, 0.2～0.3% KH2PO4, 1～3% rice bran carries
Take liquid, 2～5% sodium glutamates and make red koji fermentation culture medium, pH nature, cool down after sterilizing standby；
4）Inoculation red koji fermentation：In the monascus specie seed liquor of red koji fermentation inoculation of medium culture medium weight 7%, 26
～29 DEG C, cultivate 7～10 days under conditions of 150 ~ 180 rpm, obtain the kelp blood pressure peptide system with notable hypotensive activity
Product.
Step 2）Described in mixed enzyme be alkali protease, pepsin, trypsase, papain, local flavor albumen
One or several mixing in enzyme, cellulase, zytase, pectase.
Step 4）Described in monascus specie be monascus parpureus Went、A kind of or a kind of in M.pilosus and red monascus
More than.
Choose in described technique with kelp processing waste products degumming Kelp Powder as raw material, first mutually tied using physics, chemistry
The macromolecular structure such as polysaccharide, albumen in the method degraded sea-tangle of conjunction, then mixed enzyme bioconversion is used, finally fermentation consumes hydrolysis and produces
Raw monosaccharide component and free amino acid, improve kelp blood pressure peptide purity and activity, while producing the drop such as GABA
Blood pressure bioactive ingredients.
In red koji fermentation culture medium, the glutamate decarboxylase bioconversion sodium glutamate of rice bran, accumulate substantial amounts of drop blood
Pressure active component GABA, concentration reach 1200 ~ 3000 mg/100g.
A kind of kelp blood pressure peptides products of preparation method gained as above, biologically active peptide content be 10.5 ~
16.3 g/100g.Show through zoopery, under the disposition of high dose, it is big that kelp blood pressure peptides products reduce primary
37.5 mmHg of mouse blood pressure, with significant hypotensive activity.
The beneficial effects of the present invention is：
1）The present invention is scientifically and rationally chosen with kelp processing waste products degumming Kelp Powder as raw material, using physics, chemistry, enzyme life
Blood pressure lowering peptide prepared by the method that thing conversion and red koji fermentation combine, has not only reached the mesh of sea-tangle comprehensive utilization of resources
, purity and the activity of kelp blood pressure peptide is also improved, while producing the hypotensive bioactive ingredients such as GABA；
2）Red yeast rice used by the present invention can produce the active component GABA of lowering blood pressure and blood fat during the fermentation,
Concentration reaches 1200 ~ 3000 mg/100g；
3）In kelp blood pressure peptides products obtained in the present invention, biologically active peptide content is 10.5 ~ 16.3 g/100g.Zoopery
As a result show, under the disposition of high dose, kelp blood pressure peptides products reduce by 37.5 mmHg of primary rat blood pressure, tool
There is significant hypotensive activity.
Specific embodiment
A kind of method that red koji fermentation sea-tangle hydrolyzate prepares blood pressure lowering peptide, comprises the following steps：
1）Pretreatment of raw material：Degumming Kelp Powder and concentration are that the hydrochloric acid of 1.0～2.0 mol/L compares 1 with weight:10～1:20 mix
Closing, fully dissolving, under ultrasonic assistant effect, temperature is 45～60 DEG C of 2～6 h of hydrolysis, 5～10 is centrifuged in 4000 r/min
Min, supernatant are sea-tangle pretreatment fluid；
2）Prepared by sea-tangle hydrolyzate：The pH for adjusting sea-tangle pretreatment fluid is 7.0～8.5, adds mixing in sea-tangle pretreatment fluid
Enzyme carries out bioconversion, and mixed enzyme addition is the 0.2～3% of sea-tangle pretreatment fluid weight, and conversion temperature is 35～60 DEG C, turns
The change time is 2-8 h, obtains sea-tangle hydrolyzate after the completion of bioconversion；
3）Prepared by red koji fermentation culture medium：In sea-tangle hydrolyzate add sea-tangle hydrolyzate weight 1～2% soluble starch, 1
～2% peptone, 0.05～0.2% MgSO4·7H2O, 0.1～0.3% NaNO3, 0.2～0.3% KH2PO4, 1～3% rice bran carries
Take liquid, 2～5% sodium glutamates and make red koji fermentation culture medium, pH nature, cool down after sterilizing standby；
4）Inoculation red koji fermentation：In the monascus specie seed liquor of red koji fermentation inoculation of medium culture medium weight 7%, 26
～29 DEG C, cultivate 7～10 days under conditions of 150 ~ 180 rpm, obtain the kelp blood pressure peptide system with notable hypotensive activity
Product.
Step 2）Described in mixed enzyme be alkali protease, pepsin, trypsase, papain, local flavor albumen
One or several mixing in enzyme, cellulase, zytase, pectase.
Step 4）Described in monascus specie be monascus parpureus Went、A kind of or a kind of in M.pilosus and red monascus
More than.
The glutamate decarboxylase bioconversion sodium glutamate of rice bran in red koji fermentation culture medium, accumulates substantial amounts of hypotensive work
Property composition GABA, concentration reach 1200 ~ 3000 mg/100g.
In the kelp blood pressure peptides products of gained, biologically active peptide content is 10.5 ~ 16.3 g/100g.
The present invention further illustrates the present invention with the following example, but protection scope of the present invention is not limited to following reality
Apply example.
Embodiment 1
A kind of method that red koji fermentation sea-tangle hydrolyzate prepares blood pressure lowering peptide, comprises the following steps：
1）Pretreatment of raw material：Degumming Kelp Powder and concentration are that the hydrochloric acid of 2.0 mol/L compares 1 with weight:20 mixing, fully dissolve,
Under ultrasonic assistant effect, temperature is 50 DEG C of 3 h of hydrolysis, is centrifuged 8min in 4000 r/min, and supernatant is sea-tangle and locates in advance
Reason liquid；
2）Prepared by sea-tangle hydrolyzate：Add pepsin and the cellulase of 3wt%, wherein stomach cardia in sea-tangle pretreatment fluid
The mass ratio of enzyme and cellulase is 1:1, the pH for adjusting sea-tangle pretreatment fluid is 7.0, and conversion temperature is 35 DEG C, and transformation time is
7 h, are placed in water bath with thermostatic control and react；
3）Prepared by red koji fermentation culture medium：Add 2% soluble starch, 2% egg of sea-tangle hydrolyzate weight in sea-tangle hydrolyzate
White peptone, 0.2% MgSO4·7H2O、0.3% NaNO3、0.3% KH2PO4, 3% rice bran extract, 5% sodium glutamate make red yeast rice and send out
Ferment culture medium, pH nature, cool down after sterilizing standby；
4）Inoculation red koji fermentation：In the monascus parpureus Went bacterial classification seed liquor of red koji fermentation inoculation of medium culture medium weight 7%,
At 27 DEG C, cultivate 10 days under conditions of 180 rpm, obtain the kelp blood pressure peptides products with notable hypotensive activity.
Composition detection is carried out to gained finished product, as a result for：
In kelp blood pressure peptides products, biologically active peptide content is 16.3 g/100g；GABA concentration reaches 3000 mg/
100g.
Test basis：Function of health food assessment process and the method for inspection
Zoopery：
1 experiment material and method
1.1 experimental subjects
1.1.1 sample treatment
Test sample is the product dilution of variable concentrations, sample preparation is become each dosage with physiological saline during experiment.
1.1.2 animal used as test and detection environmental condition
Experiment original hypertensive rat（SHR）, male, healthy cleaning grade, 160 ~ 200 g of body weight, totally 40, by Fujian
Medical university's Experimental Animal Center is provided.Detection environmental condition, 25 DEG C of temperature range, RH range 70%, standard feed are fed
Support, ad lib and drinking-water, rat adapts to one week in Animal House before the test.
The packet of 1.2 dosage and given the test agent give the time
Experiment sets three dosage groups, a negative control group and positive controls, and the tested time is 28 d.
1.3 experimental procedure
SHR rat according to surveyed basic blood pressure and body weight, 40 is randomly divided into 5 groups after laboratory adapts to one week, per group 8
Only, it is respectively：Blank control group（Physiological saline）, three groups of the high, medium and low dosage of kelp blood pressure peptide (is followed successively by 80,40,20
), and positive controls mg/kg（5 mg/kg of captopril）.Gavage gives corresponding tested material to each group respectively, once a day, even
Continue and give 4 weeks.Administration determines weekly the blood pressure of a rat after terminating, be repeated three times every time and average, the survey of blood pressure
Surely shrink platen press using rat tail artery to determine.Experiment the last week carries out multiple blood pressure measurement to tested rat, so as to its adaptation
Pressure measurement environment.
1.4 experimental datas are counted
Data summarization and analysis are carried out using spss19.0 and sigmaplot11.0 software.
2 experimental results
Impact of the 2.1 kelp blood pressure peptides products to rat blood pressure
Impact of the 1 kelp blood pressure peptides products of table to rat blood pressure
From table 1, before rat gives tested material (0 d), each experimental group blood pressure content is compared nothing conspicuousness with blank control group
Difference (p＞ 0.05).In the fed SHR regular period（7、14、21、28 d）Afterwards, each dosage group with to before tested material and
Blank group compares, the difference of blood pressure have conspicuousness (p＜ 0.01), with the prolongation of the time of continued administration, SHR rat blood pressure declines
Trend slow down and gradually tend towards stability.When testing to 28 d, contrast with blank group, low dose group have dropped 21.4 mmHg,
Middle dose group have dropped 30.5 mmHg, and high dose group have dropped 37.5 mmHg.Show that the kelp blood pressure peptides products have bright
The aobvious effect for reducing blood pressure.
Impact of the 2.2 kelp blood pressure peptides products to rat body weight
Impact of the 2 kelp blood pressure peptides products of table to rat body weight
From table 2, before rat is to tested material（0 d）, each group body weight is not significantly different from.To the tested material regular period (7,14
, 21,28 d) after, compared with blank control group, remaining each group body weight all do not occur significant difference (p＞ 0.05).Show the sea
Band blood pressure lowering peptide product does not have harmful effect to rat growthing development.
Impact of the 2.3 kelp blood pressure peptides products to rat heart rate
Impact of the 3 kelp blood pressure peptides products of table to rat heart rate
From table 3, SHR rat before administration after changes in heart rate all do not occur significant difference (p＞ 0.05).Show the sea-tangle drop
Blood pressure peptides products do not have harmful effect to rat growthing development.
3 conclusions
In sum, before and after experiment the body weight of SHR rat, changes in heart rate all do not occur significant difference (p＞ 0.05).Show the sea
Band blood pressure lowering peptide product does not have harmful effect to rat growthing development.And although the SHR rat blood pressure of each dosage group is general compared with Kato
The antihypertensive effect of profit group is poor, but still in certain downward trend, wherein, low dose group declines 21.4 mmHg, and middle dose group declines
30.5 mmHg, high dose group decline 37.5 mmHg.High dose group illustrates that the kelp blood pressure peptides products have significant drop blood
Pressure activity.
Embodiment 2
A kind of method that red koji fermentation sea-tangle hydrolyzate prepares blood pressure lowering peptide, comprises the following steps：
1）Pretreatment of raw material：Degumming Kelp Powder and concentration compare 1 for the hydrochloric acid of 1.0mol/L with weight:10 mixing, fully dissolve,
Under ultrasonic assistant effect, temperature is 45 DEG C of 5 h of hydrolysis, is centrifuged 10 min in 4000 r/min, and supernatant is sea-tangle and locates in advance
Reason liquid；
2）Prepared by sea-tangle hydrolyzate：Add alkali protease, flavor protease and the pectase of 1wt% in sea-tangle pretreatment fluid,
The weight ratio of wherein alkali protease, flavor protease and pectase is 1:1:2, the pH for adjusting sea-tangle pretreatment fluid is 8.5, turns
It is 50 DEG C to change temperature, and 5 h of transformation time is placed in water bath with thermostatic control and reacts；
3）Prepared by red koji fermentation culture medium：Add 1% soluble starch, 1% egg of sea-tangle hydrolyzate weight in sea-tangle hydrolyzate
White peptone, 0.05% MgSO4·7H2O、0.1% NaNO3、0.2% KH2PO4, 1% rice bran extract, 3% sodium glutamate make red yeast rice and send out
Ferment culture medium, pH nature, cool down after sterilizing standby；
4）Inoculation red koji fermentation：In the Monascus ruber mould species seed liquor of red koji fermentation inoculation of medium culture medium weight 7%,
At 29 DEG C, cultivate 10 days under conditions of 150 rpm, obtain the kelp blood pressure peptides products with notable hypotensive activity.
Composition detection is carried out to gained finished product, as a result for：
In kelp blood pressure peptides products, biologically active peptide content is 14.5 g/100g；GABA concentration reaches 2200mg/
100g.
Test basis are with embodiment 1.Experimental result shows that the body weight of SHR rat, changes in heart rate all do not occur before and after experiment
Significant difference (p＞ 0.05).Show that the kelp blood pressure peptides products are had no adverse effects to rat growthing development.And each dosage group
SHR rat blood pressure situation of change is：Low dose group declines 14.6 mmHg, and middle dose group declines 21.3 mmHg, under high dose group
29.8 mmHg drop.Illustrate that the kelp blood pressure peptides products have significant hypotensive activity.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes that is done according to scope of the present invention patent with
Modification, should all belong to the covering scope of the present invention.
Claims (5)
1. a kind of method that red koji fermentation sea-tangle hydrolyzate prepares blood pressure lowering peptide, it is characterised in that comprise the following steps：
1）Pretreatment of raw material：Degumming Kelp Powder and concentration are that the hydrochloric acid of 1.0～2.0 mol/L compares 1 with weight:10～1:20 mix
Closing, fully dissolving, under ultrasonic assistant effect, temperature is 45～60 DEG C of 2～6 h of hydrolysis, 5～10 is centrifuged in 4000 r/min
Min, supernatant are sea-tangle pretreatment fluid；
2）Prepared by sea-tangle hydrolyzate：The pH for adjusting sea-tangle pretreatment fluid is 7.0～8.5, adds mixing in sea-tangle pretreatment fluid
Enzyme carries out bioconversion, and mixed enzyme addition is the 0.2～3% of sea-tangle pretreatment fluid weight, and conversion temperature is 35～60 DEG C, turns
The change time is 2 ~ 8 h, obtains sea-tangle hydrolyzate after the completion of bioconversion；
3）Prepared by red koji fermentation culture medium：In sea-tangle hydrolyzate add sea-tangle hydrolyzate weight 1～2% soluble starch, 1
～2% peptone, 0.05～0.2% MgSO4·7H2O, 0.1～0.3% NaNO3, 0.2～0.3% KH2PO4, 1～3% rice bran carries
Take liquid, 2～5% sodium glutamates and make red koji fermentation culture medium, pH nature, cool down after sterilizing standby；
4）Inoculation red koji fermentation：In the monascus specie seed liquor of red koji fermentation inoculation of medium culture medium weight 7%, 26
～29 DEG C, cultivate 7～10 days under conditions of 150 ~ 180 rpm, obtain the kelp blood pressure peptide system with notable hypotensive activity
Product.
2. the method that a kind of red koji fermentation sea-tangle hydrolyzate according to claim 1 prepares blood pressure lowering peptide, it is characterised in that：
Step 2）Described in mixed enzyme be alkali protease, pepsin, trypsase, papain, flavor protease, cellulose
One or several mixing in enzyme, zytase, pectase.
3. the method that a kind of red koji fermentation sea-tangle hydrolyzate according to claim 1 prepares blood pressure lowering peptide, it is characterised in that：
Step 4）Described in monascus specie be monascus parpureus Went、In M.pilosus and red monascus one or more.
4. the method that a kind of red koji fermentation sea-tangle hydrolyzate according to claim 1 prepares blood pressure lowering peptide, it is characterised in that：
The glutamate decarboxylase bioconversion sodium glutamate of rice bran in red koji fermentation culture medium, accumulates substantial amounts of hypotensive activity composition
GABA, concentration reach 1200 ~ 3000 mg/100g.
5. the method that a kind of red koji fermentation sea-tangle hydrolyzate according to claim 1 prepares blood pressure lowering peptide, it is characterised in that：
In the kelp blood pressure peptides products of gained, biologically active peptide content is 10.5 ~ 16.3 g/100g.
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| JP2020115785A (en) * | 2019-01-24 | 2020-08-06 | 濱田 奈保子 | Immunomodulating composition and method for producing the same |
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| JP2020115785A (en) * | 2019-01-24 | 2020-08-06 | 濱田 奈保子 | Immunomodulating composition and method for producing the same |
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