CN107109489B - 纳米孔rna表征方法 - Google Patents

纳米孔rna表征方法 Download PDF

Info

Publication number
CN107109489B
CN107109489B CN201580069027.9A CN201580069027A CN107109489B CN 107109489 B CN107109489 B CN 107109489B CN 201580069027 A CN201580069027 A CN 201580069027A CN 107109489 B CN107109489 B CN 107109489B
Authority
CN
China
Prior art keywords
rna
dna
polynucleotide
rna polynucleotide
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201580069027.9A
Other languages
English (en)
Chinese (zh)
Other versions
CN107109489A (zh
Inventor
丹尼尔·赖安·加拉尔达
安德鲁·约翰·赫伦
拉科马·贾亚辛格
丹尼尔·约翰·特纳
詹姆斯·怀特
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford Nanopore Technologies PLC
Original Assignee
Oxford Nanopore Technologies PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB201418459A external-priority patent/GB201418459D0/en
Priority claimed from GBGB1508270.4A external-priority patent/GB201508270D0/en
Priority claimed from GBGB1517634.0A external-priority patent/GB201517634D0/en
Priority claimed from PCT/GB2015/052916 external-priority patent/WO2016055777A2/en
Application filed by Oxford Nanopore Technologies PLC filed Critical Oxford Nanopore Technologies PLC
Priority to CN202111321280.2A priority Critical patent/CN113981055A/zh
Publication of CN107109489A publication Critical patent/CN107109489A/zh
Application granted granted Critical
Publication of CN107109489B publication Critical patent/CN107109489B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/04—Hydrolases acting on acid anhydrides (3.6) acting on acid anhydrides; involved in cellular and subcellular movement (3.6.4)
    • C12Y306/04012—DNA helicase (3.6.4.12)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/513—Winding/unwinding enzyme, e.g. helicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/121—Modifications characterised by incorporating both deoxyribonucleotides and ribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/197—Modifications characterised by incorporating a spacer/coupling moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Electrochemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201580069027.9A 2014-10-17 2015-10-19 纳米孔rna表征方法 Active CN107109489B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111321280.2A CN113981055A (zh) 2014-10-17 2015-10-19 纳米孔rna表征方法

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
GB1418459.2 2014-10-17
GB201418459A GB201418459D0 (en) 2014-10-17 2014-10-17 Method
GBGB1508270.4A GB201508270D0 (en) 2015-05-14 2015-05-14 Method
GB1508270.4 2015-05-14
GBPCT/GB2015/052916 2015-10-06
GBGB1517634.0A GB201517634D0 (en) 2015-10-06 2015-10-06 Method
PCT/GB2015/052916 WO2016055777A2 (en) 2014-10-07 2015-10-06 Modified enzymes
GB1517634.0 2015-10-06
PCT/GB2015/053097 WO2016059436A1 (en) 2014-10-17 2015-10-19 Method for nanopore rna characterisation

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202111321280.2A Division CN113981055A (zh) 2014-10-17 2015-10-19 纳米孔rna表征方法

Publications (2)

Publication Number Publication Date
CN107109489A CN107109489A (zh) 2017-08-29
CN107109489B true CN107109489B (zh) 2021-11-30

Family

ID=54337815

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201580069027.9A Active CN107109489B (zh) 2014-10-17 2015-10-19 纳米孔rna表征方法
CN202111321280.2A Pending CN113981055A (zh) 2014-10-17 2015-10-19 纳米孔rna表征方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202111321280.2A Pending CN113981055A (zh) 2014-10-17 2015-10-19 纳米孔rna表征方法

Country Status (6)

Country Link
US (4) US10480026B2 (enExample)
EP (1) EP3207155B1 (enExample)
JP (1) JP6824881B2 (enExample)
KR (1) KR102457147B1 (enExample)
CN (2) CN107109489B (enExample)
WO (1) WO2016059436A1 (enExample)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
AU2013220156B2 (en) 2012-02-15 2018-08-09 Oxford Nanopore Technologies Limited Aptamer method
CN112646019B (zh) 2012-04-10 2022-08-16 牛津纳米孔科技公开有限公司 突变胞溶素孔
WO2013185137A1 (en) 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
AU2013291763B2 (en) 2012-07-19 2019-06-20 Oxford Nanopore Technologies Limited Modified helicases
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
CA2927726A1 (en) 2013-10-18 2015-04-23 Oxford Nanopore Technologies Limited Modified helicases for use in nucleic acid sequencing
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
CN107109489B (zh) 2014-10-17 2021-11-30 牛津纳米孔技术公司 纳米孔rna表征方法
KR102222192B1 (ko) 2016-03-02 2021-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 기공
US11514289B1 (en) * 2016-03-09 2022-11-29 Freenome Holdings, Inc. Generating machine learning models using genetic data
JP7364333B2 (ja) 2016-04-06 2023-10-18 オックスフォード ナノポール テクノロジーズ ピーエルシー 変異体ポア
GB201609221D0 (en) * 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
WO2017203268A1 (en) * 2016-05-25 2017-11-30 Oxford Nanopore Technologies Limited Method
GB201617886D0 (en) * 2016-10-21 2016-12-07 Oxford Nanopore Technologies Limited Method
GB201620450D0 (en) * 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
WO2018200847A1 (en) * 2017-04-27 2018-11-01 The Regents Of The University Of California Methods of producing ribosomal ribonucleic acid complexes
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
KR102593484B1 (ko) * 2017-06-29 2023-10-24 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 나노포어를 통한 폴리머의 결정적 스텝핑
CN117106037B (zh) 2017-06-30 2025-07-25 弗拉芒区生物技术研究所 新颖蛋白孔
GB2569977A (en) * 2018-01-05 2019-07-10 Oxford Nanopore Tech Ltd Method
WO2019149626A1 (de) 2018-02-02 2019-08-08 Bayer Aktiengesellschaft Bekämpfung resistenter schadorganismen
WO2019226822A1 (en) * 2018-05-23 2019-11-28 The Regents Of The University Of California Methods of analyzing capped ribonucleic acids
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
EP3650558A1 (en) * 2018-11-07 2020-05-13 Siemens Healthcare GmbH Liquid sample workflow for nanopore sequencing
ES2927412T3 (es) * 2018-11-08 2022-11-04 Siemens Healthcare Gmbh Secuenciación directa de nanoporos de ARN con la ayuda de un polinucleótido de horquilla
CN111455031A (zh) * 2019-01-18 2020-07-28 中国科学院微生物研究所 基于Nanopore测序技术的多组学测序及分析方法
CN110093399A (zh) * 2019-04-22 2019-08-06 武汉大学 一种利用二磷酸尿嘧啶脱氧核苷酸检测核酸中腺嘌呤n6位发生甲基化修饰的方法
GB201907244D0 (en) * 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
US11926819B2 (en) 2019-05-28 2024-03-12 The Regents Of The University Of California Methods of adding polymers to ribonucleic acids
WO2021036995A1 (en) * 2019-08-23 2021-03-04 Nanjing University Direct microrna sequencing using enzyme assisted sequencing
CN110568039B (zh) * 2019-09-06 2021-05-04 中国科学院生物物理研究所 使用新型电化学传感器的氨基酸特异实时检测方法
CN111164097B (zh) * 2019-09-29 2021-02-19 北京齐碳科技有限公司 一种Mnep单体变体及其应用
CN113930406B (zh) * 2021-12-17 2022-04-08 北京齐碳科技有限公司 一种Pif1-like解旋酶及其应用
GB202107192D0 (en) * 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method
CN114134142B (zh) * 2021-11-23 2024-05-28 成都齐碳科技有限公司 衔接体、复合物、单链分子、试剂盒、方法和应用
CN116497102A (zh) * 2021-12-21 2023-07-28 成都齐碳科技有限公司 用于表征目标多核苷酸的衔接体、方法及其用途
GB202204919D0 (en) 2022-04-04 2022-05-18 Oxford Nanopore Tech Plc Method
CN119213145A (zh) * 2022-05-27 2024-12-27 深圳华大生命科学研究院 一种维持纳米孔测序速度的方法
WO2024099985A1 (en) 2022-11-10 2024-05-16 Bayer Aktiengesellschaft Targeted crop protection product application based on genetic profiles
CN116478983B (zh) * 2022-11-21 2023-10-24 北京普译生物科技有限公司 一种rna-dna嵌合接头及其应用
CN116622822A (zh) * 2023-03-17 2023-08-22 四川大学 一种多重混样直接rna纳米孔测序方法

Family Cites Families (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
CA2128891A1 (en) 1992-01-29 1993-08-05 Cylia Keller Polynucleotide immobilized support
US6426231B1 (en) 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
DE60025739T2 (de) 1999-09-07 2006-08-31 The Regents Of The University Of California, Oakland Verfahren um die anwesenheit von doppelsträngiger dns in einer probe nachzuweisen
EP1097993A3 (en) * 1999-11-05 2004-01-07 National Institute of Advanced Industrial Science and Technology Functional ribozyme chimeric molecules capable of sliding
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
US6706203B2 (en) 2001-10-30 2004-03-16 Agilent Technologies, Inc. Adjustable nanopore, nanotome, and nanotweezer
US20040029158A1 (en) 2002-05-17 2004-02-12 Eric Olson HOP - a novel cardiac-restricted transcriptional factor potentially useful for cardiac regeneration and specification
AU2003272438B2 (en) * 2002-09-20 2009-04-02 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
CN101245376A (zh) 2003-01-17 2008-08-20 香港中文大学 作为妊娠相关病症的诊断标志物的循环mRNA
US20050250094A1 (en) 2003-05-30 2005-11-10 Nanosphere, Inc. Method for detecting analytes based on evanescent illumination and scatter-based detection of nanoparticle probe complexes
US7347921B2 (en) 2003-07-17 2008-03-25 Agilent Technologies, Inc. Apparatus and method for threading a biopolymer through a nanopore
US20060030535A1 (en) 2004-03-05 2006-02-09 Healy Judith M Controlled modulation of the pharmacokinetics and biodistribution of aptamer therapeutics
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
US9233846B2 (en) 2005-10-14 2016-01-12 The Regents Of The University Of California Formation and encapsulation of molecular bilayer and monolayer membranes
EP1954838B1 (en) 2005-11-14 2014-02-26 Life Technologies Corporation Coded molecules for detecting target analytes
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
US20070224613A1 (en) 2006-02-18 2007-09-27 Strathmann Michael P Massively Multiplexed Sequencing
WO2007117832A2 (en) 2006-03-12 2007-10-18 Applera Corporation Methods of detecting target nucleic acids
WO2008066621A2 (en) 2006-10-19 2008-06-05 Duke University Reversible platelet inhibition
NZ579083A (en) 2007-02-20 2012-07-27 Oxford Nanopore Tech Ltd Lipid bilayer sensor system
AU2008236694B2 (en) 2007-04-04 2014-01-23 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
WO2009046149A1 (en) 2007-10-01 2009-04-09 Applied Biosystems Inc. Chase ligation sequencing
ES2448426T3 (es) 2007-10-12 2014-03-13 Pronota Nv Uso de aptámeros en proteómica
WO2009053691A1 (en) 2007-10-22 2009-04-30 Pronota Nv Method of selecting aptamers
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US20110263459A1 (en) 2008-03-12 2011-10-27 Borer Philip N Direct selection of structurally defined aptamers
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US9447152B2 (en) 2008-07-07 2016-09-20 Oxford Nanopore Technologies Limited Base-detecting pore
US20110229877A1 (en) 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
US20100092960A1 (en) 2008-07-25 2010-04-15 Pacific Biosciences Of California, Inc. Helicase-assisted sequencing with molecular beacons
WO2010047797A2 (en) 2008-10-21 2010-04-29 Research Foundation Of The City University Of New York Single quantum-dot-based aptameric sensors
JP2012507565A (ja) * 2008-10-30 2012-03-29 グオ・ペイシュエン Ｄｎａのシークエンシング及び他の用途のための、膜に組み込まれたウイルスｄｎａパッケージングモータタンパク質コネクタバイオセンサ
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
CA2750874A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Hybridization linkers
JP5843614B2 (ja) 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
US8986928B2 (en) 2009-04-10 2015-03-24 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
JP5781502B2 (ja) 2009-04-20 2015-09-24 オックスフォード ナノポール テクノロジーズ リミテッド 脂質二重層センサアレイ
BR112012013074B1 (pt) 2009-12-01 2018-09-18 Oxford Nanopore Technologies Limited instrumento de análise e módulo para realizar análise bioquímica, e, método para operar um instrumento de análise para realizar análise bioquímica
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
WO2011103424A2 (en) 2010-02-19 2011-08-25 The Trustees Of The University Of Pennsylvania High-resolution analysis devices and related methods
US8557529B2 (en) 2010-04-09 2013-10-15 International Business Machines Corporation Nanopore capture system
JP6016792B2 (ja) 2010-07-14 2016-10-26 ザ キュレイターズ オブ ザ ユニバーシティ オブ ミズーリ ナノポア促進型の核酸の単分子検出
WO2012033524A2 (en) * 2010-09-07 2012-03-15 The Regents Of The University Of California Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme
GB2500360B (en) 2010-12-22 2019-10-23 Genia Tech Inc Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps
KR101939420B1 (ko) 2011-02-11 2019-01-16 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 세공
US9290815B2 (en) * 2011-02-21 2016-03-22 Qiagen Gmbh Method for quantifying human DNA
EP2689028B1 (en) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
AU2012264497B2 (en) 2011-05-27 2017-06-15 Oxford Nanopore Technologies Limited Coupling method
US10228347B2 (en) * 2011-06-24 2019-03-12 Electronic Biosciences, Inc. High contrast signal to noise ratio device components
JP6298404B2 (ja) * 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
JP2014521956A (ja) 2011-07-27 2014-08-28 ザ ボード オブ トラスティーズ オブ ザ ユニヴァーシティー オブ イリノイ 生体分子を特徴付けるためのナノ細孔センサー
EP2769007B1 (en) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
JP6226869B2 (ja) * 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
CN102430153A (zh) * 2011-12-24 2012-05-02 中国人民解放军第三军医大学第二附属医院 一种基于交联技术的仿生骨界面、构建方法及其应用
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
KR102086182B1 (ko) 2011-12-29 2020-03-06 옥스포드 나노포어 테크놀로지즈 리미티드 효소 방법
AU2013220156B2 (en) 2012-02-15 2018-08-09 Oxford Nanopore Technologies Limited Aptamer method
BR102012007246A2 (pt) 2012-03-30 2016-02-10 Fundação Antonio Prudente métodos para prognóstico e classificação de resultados de um evento
CN112646019B (zh) 2012-04-10 2022-08-16 牛津纳米孔科技公开有限公司 突变胞溶素孔
AU2013291763B2 (en) * 2012-07-19 2019-06-20 Oxford Nanopore Technologies Limited Modified helicases
US9797009B2 (en) 2012-07-19 2017-10-24 Oxford Nanopore Technologies Limited Enzyme construct
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
EP2895618B1 (en) 2012-09-14 2017-07-26 Oxford Nanopore Technologies Limited Sample preparation method
US9995728B2 (en) 2012-11-06 2018-06-12 Oxford Nanopore Technologies Ltd. Quadruplex method
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
AU2014224432B2 (en) 2013-03-08 2019-10-24 Oxford Nanopore Technologies Limited Enzyme stalling method
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CA2927726A1 (en) 2013-10-18 2015-04-23 Oxford Nanopore Technologies Limited Modified helicases for use in nucleic acid sequencing
EP3126515B1 (en) 2014-04-04 2018-08-29 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
CN107109489B (zh) 2014-10-17 2021-11-30 牛津纳米孔技术公司 纳米孔rna表征方法

Also Published As

Publication number Publication date
US20200063199A1 (en) 2020-02-27
JP6824881B2 (ja) 2021-02-03
EP3207155B1 (en) 2019-06-05
WO2016059436A1 (en) 2016-04-21
KR102457147B1 (ko) 2022-10-19
US20250084472A1 (en) 2025-03-13
US10480026B2 (en) 2019-11-19
CN107109489A (zh) 2017-08-29
KR20170072915A (ko) 2017-06-27
CN113981055A (zh) 2022-01-28
US11021747B2 (en) 2021-06-01
JP2017532962A (ja) 2017-11-09
EP3207155A1 (en) 2017-08-23
US20170253923A1 (en) 2017-09-07
US20210395811A1 (en) 2021-12-23
US12129518B2 (en) 2024-10-29
US20230012471A9 (en) 2023-01-12

Similar Documents

Publication Publication Date Title
CN107109489B (zh) 纳米孔rna表征方法
US12473593B2 (en) Method of characterizing a target ribonucleic acid (RNA) comprising forming a complementary polynucleotide which moves through a transmembrane pore
US11542551B2 (en) Sample preparation method
CN110088299B (zh) 通过纳米孔指导的核酸检测方法
EP2895618B1 (en) Sample preparation method
EP3033435B1 (en) Method for fragmenting nucleic acid by means of transposase

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
TG01 Patent term adjustment
TG01 Patent term adjustment