KR102457147B1 - 나노 세공 rna 특징 규명을 위한 방법 - Google Patents

나노 세공 rna 특징 규명을 위한 방법 Download PDF

Info

Publication number
KR102457147B1
KR102457147B1 KR1020177013136A KR20177013136A KR102457147B1 KR 102457147 B1 KR102457147 B1 KR 102457147B1 KR 1020177013136 A KR1020177013136 A KR 1020177013136A KR 20177013136 A KR20177013136 A KR 20177013136A KR 102457147 B1 KR102457147 B1 KR 102457147B1
Authority
KR
South Korea
Prior art keywords
rna
dna
polynucleotide
seq
rna polynucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020177013136A
Other languages
English (en)
Korean (ko)
Other versions
KR20170072915A (ko
Inventor
다니엘 라이언 제럴드
앤드류 존 헤론
라크말 자야징헤
다니엘 존 터너
제임스 화이트
Original Assignee
옥스포드 나노포어 테크놀로지즈 피엘씨
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB201418459A external-priority patent/GB201418459D0/en
Priority claimed from GBGB1508270.4A external-priority patent/GB201508270D0/en
Priority claimed from GBGB1517634.0A external-priority patent/GB201517634D0/en
Priority claimed from PCT/GB2015/052916 external-priority patent/WO2016055777A2/en
Application filed by 옥스포드 나노포어 테크놀로지즈 피엘씨 filed Critical 옥스포드 나노포어 테크놀로지즈 피엘씨
Publication of KR20170072915A publication Critical patent/KR20170072915A/ko
Application granted granted Critical
Publication of KR102457147B1 publication Critical patent/KR102457147B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/04—Hydrolases acting on acid anhydrides (3.6) acting on acid anhydrides; involved in cellular and subcellular movement (3.6.4)
    • C12Y306/04012—DNA helicase (3.6.4.12)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/513—Winding/unwinding enzyme, e.g. helicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/121—Modifications characterised by incorporating both deoxyribonucleotides and ribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/197—Modifications characterised by incorporating a spacer/coupling moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
KR1020177013136A 2014-10-17 2015-10-19 나노 세공 rna 특징 규명을 위한 방법 Active KR102457147B1 (ko)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
GB201418459A GB201418459D0 (en) 2014-10-17 2014-10-17 Method
GB1418459.2 2014-10-17
GBGB1508270.4A GB201508270D0 (en) 2015-05-14 2015-05-14 Method
GB1508270.4 2015-05-14
GBGB1517634.0A GB201517634D0 (en) 2015-10-06 2015-10-06 Method
GBPCT/GB2015/052916 2015-10-06
PCT/GB2015/052916 WO2016055777A2 (en) 2014-10-07 2015-10-06 Modified enzymes
GB1517634.0 2015-10-06
PCT/GB2015/053097 WO2016059436A1 (en) 2014-10-17 2015-10-19 Method for nanopore rna characterisation

Publications (2)

Publication Number Publication Date
KR20170072915A KR20170072915A (ko) 2017-06-27
KR102457147B1 true KR102457147B1 (ko) 2022-10-19

Family

ID=54337815

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020177013136A Active KR102457147B1 (ko) 2014-10-17 2015-10-19 나노 세공 rna 특징 규명을 위한 방법

Country Status (6)

Country Link
US (4) US10480026B2 (enExample)
EP (1) EP3207155B1 (enExample)
JP (1) JP6824881B2 (enExample)
KR (1) KR102457147B1 (enExample)
CN (2) CN113981055A (enExample)
WO (1) WO2016059436A1 (enExample)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
CN104039979B (zh) 2011-10-21 2016-08-24 牛津纳米孔技术公司 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法
CA2864035C (en) 2012-02-15 2021-05-18 Oxford Nanopore Technologies Limited A method for determining the presence of an analyte using an aptamer
WO2013153359A1 (en) 2012-04-10 2013-10-17 Oxford Nanopore Technologies Limited Mutant lysenin pores
GB2559073A (en) 2012-06-08 2018-07-25 Pacific Biosciences California Inc Modified base detection with nanopore sequencing
AU2013291763B2 (en) 2012-07-19 2019-06-20 Oxford Nanopore Technologies Limited Modified helicases
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
EP3575410A3 (en) 2013-10-18 2020-03-04 Oxford Nanopore Technologies Limited Modified enzymes
GB201417712D0 (en) * 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
CN119899249A (zh) 2014-09-01 2025-04-29 弗拉芒区生物技术研究所 突变csgg孔
WO2016059436A1 (en) 2014-10-17 2016-04-21 Oxford Nanopore Technologies Limited Method for nanopore rna characterisation
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
KR102222191B1 (ko) 2016-03-02 2021-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 기공
US11514289B1 (en) * 2016-03-09 2022-11-29 Freenome Holdings, Inc. Generating machine learning models using genetic data
CN118272512A (zh) 2016-04-06 2024-07-02 牛津纳米孔科技公开有限公司 突变体孔
EP3464616B1 (en) * 2016-05-25 2022-05-04 Oxford Nanopore Technologies plc Method
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201617886D0 (en) * 2016-10-21 2016-12-07 Oxford Nanopore Technologies Limited Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
US11578362B2 (en) * 2017-04-27 2023-02-14 The Regents Of The University Of California Methods of producing ribosomal ribonucleic acid complexes
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
KR102885764B1 (ko) * 2017-06-29 2025-11-13 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 나노포어를 통한 폴리머의 결정적 스텝핑
EP3645552B1 (en) 2017-06-30 2023-06-28 Vib Vzw Novel protein pores
GB2569977A (en) * 2018-01-05 2019-07-10 Oxford Nanopore Tech Ltd Method
WO2019149626A1 (de) 2018-02-02 2019-08-08 Bayer Aktiengesellschaft Bekämpfung resistenter schadorganismen
US12195794B2 (en) 2018-05-23 2025-01-14 The Regents Of The University Of California Methods of analyzing capped ribonucleic acids
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
EP3650558A1 (en) * 2018-11-07 2020-05-13 Siemens Healthcare GmbH Liquid sample workflow for nanopore sequencing
ES2927412T3 (es) * 2018-11-08 2022-11-04 Siemens Healthcare Gmbh Secuenciación directa de nanoporos de ARN con la ayuda de un polinucleótido de horquilla
CN111455031A (zh) * 2019-01-18 2020-07-28 中国科学院微生物研究所 基于Nanopore测序技术的多组学测序及分析方法
CN110093399A (zh) * 2019-04-22 2019-08-06 武汉大学 一种利用二磷酸尿嘧啶脱氧核苷酸检测核酸中腺嘌呤n6位发生甲基化修饰的方法
GB201907244D0 (en) * 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
US11926819B2 (en) 2019-05-28 2024-03-12 The Regents Of The University Of California Methods of adding polymers to ribonucleic acids
WO2021036995A1 (en) * 2019-08-23 2021-03-04 Nanjing University Direct microrna sequencing using enzyme assisted sequencing
CN110568039B (zh) * 2019-09-06 2021-05-04 中国科学院生物物理研究所 使用新型电化学传感器的氨基酸特异实时检测方法
CN111164097B (zh) * 2019-09-29 2021-02-19 北京齐碳科技有限公司 一种Mnep单体变体及其应用
CN113930406B (zh) * 2021-12-17 2022-04-08 北京齐碳科技有限公司 一种Pif1-like解旋酶及其应用
MX2023001142A (es) 2020-07-30 2023-05-25 Cambridge Epigenetix Ltd Composiciones y metodos para analisis de acidos nucleicos.
GB202107192D0 (en) * 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method
CN114134142B (zh) * 2021-11-23 2024-05-28 成都齐碳科技有限公司 衔接体、复合物、单链分子、试剂盒、方法和应用
WO2023116575A1 (zh) * 2021-12-21 2023-06-29 成都齐碳科技有限公司 用于表征目标多核苷酸的衔接体、方法及其用途
GB202204919D0 (en) 2022-04-04 2022-05-18 Oxford Nanopore Tech Plc Method
CN119213145A (zh) * 2022-05-27 2024-12-27 深圳华大生命科学研究院 一种维持纳米孔测序速度的方法
CN120457488A (zh) 2022-11-10 2025-08-08 拜耳公司 基于遗传谱的靶向性作物保护产品施用
CN116478983B (zh) * 2022-11-21 2023-10-24 北京普译生物科技有限公司 一种rna-dna嵌合接头及其应用
CN116622822A (zh) * 2023-03-17 2023-08-22 四川大学 一种多重混样直接rna纳米孔测序方法

Family Cites Families (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
MX9300492A (es) 1992-01-29 1994-07-29 Hitachi Chemical Co Ltd Soporte inmovilizado de nucleotido y metodo para su produccion.
US6426231B1 (en) 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
EP1212462B1 (en) 1999-09-07 2006-01-25 The Regents of the University of California Methods of determining the presence of double stranded nucleic acids in a sample
EP1097993A3 (en) * 1999-11-05 2004-01-07 National Institute of Advanced Industrial Science and Technology Functional ribozyme chimeric molecules capable of sliding
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
US6706203B2 (en) 2001-10-30 2004-03-16 Agilent Technologies, Inc. Adjustable nanopore, nanotome, and nanotweezer
WO2004041843A2 (en) 2002-05-17 2004-05-21 Board Of Regents, The University Of Texas System Hop - a novel cardiac-restricted transcriptional factor potentially useful for cardiac regeneration and specification
AU2003272438B2 (en) * 2002-09-20 2009-04-02 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
CN100379882C (zh) 2003-01-17 2008-04-09 香港中文大学 作为妊娠相关病症的诊断标志物的循环mRNA
US20050250094A1 (en) 2003-05-30 2005-11-10 Nanosphere, Inc. Method for detecting analytes based on evanescent illumination and scatter-based detection of nanoparticle probe complexes
US7347921B2 (en) 2003-07-17 2008-03-25 Agilent Technologies, Inc. Apparatus and method for threading a biopolymer through a nanopore
EP1732571A4 (en) 2004-03-05 2009-09-09 Archemix Corp CONTROLLED MODULATION OF PHARMACOKINETICS AND BIODISTRIBUTION OF APTAMER THERAPEUTICS
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
US9233846B2 (en) 2005-10-14 2016-01-12 The Regents Of The University Of California Formation and encapsulation of molecular bilayer and monolayer membranes
US20070190543A1 (en) 2005-11-14 2007-08-16 Applera Corporation Coded Molecules for Detecting Target Analytes
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
WO2007098427A2 (en) 2006-02-18 2007-08-30 Michael Strathmann Massively multiplexed sequencing
US20100291548A1 (en) 2006-03-12 2010-11-18 Applera Corporation Methods of Detecting Target Nucleic Acids
US20110118187A1 (en) 2006-10-19 2011-05-19 Duke University Reversible platelet inhibition
EP2122344B8 (en) 2007-02-20 2019-08-21 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
WO2008124107A1 (en) 2007-04-04 2008-10-16 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
US20090191553A1 (en) 2007-10-01 2009-07-30 Applied Biosystems Inc. Chase Ligation Sequencing
ES2448426T3 (es) 2007-10-12 2014-03-13 Pronota Nv Uso de aptámeros en proteómica
EP2209914B2 (en) 2007-10-22 2017-07-26 Caris Life Sciences Switzerland Holdings GmbH Method of selecting aptamers
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
CN102066562A (zh) 2008-03-12 2011-05-18 雪城大学 结构限定的适配子的直接选择
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US20110229877A1 (en) 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
CA2730068A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
US20100092960A1 (en) 2008-07-25 2010-04-15 Pacific Biosciences Of California, Inc. Helicase-assisted sequencing with molecular beacons
US8642260B2 (en) 2008-10-21 2014-02-04 The Research Foundation Of The City University Of New York Single quantum-dot based aptameric nanosensors
AU2009320006A1 (en) * 2008-10-30 2010-06-03 Peixuan Guo Membrane-integrated viral DNA-packaging motor protein connector biosensor for DNA sequencing and other uses
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
CN102482330A (zh) 2009-01-30 2012-05-30 牛津纳米孔技术有限公司 酶突变体
EP2391732B1 (en) 2009-01-30 2015-05-27 Oxford Nanopore Technologies Limited Methods using adaptors for nucleic acid constructs in transmembrane sequencing
GB0905140D0 (en) * 2009-03-25 2009-05-06 Isis Innovation Method
WO2010117470A2 (en) 2009-04-10 2010-10-14 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
CA2758617C (en) 2009-04-20 2017-10-03 Oxford Nanopore Technologies Limited Lipid bilayer sensor array
EP2507387B1 (en) 2009-12-01 2017-01-25 Oxford Nanopore Technologies Limited Biochemical analysis instrument and method
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
EP2537026B1 (en) 2010-02-19 2018-06-27 The Trustees Of The University Of Pennsylvania High-resolution analysis devices and related methods
US8557529B2 (en) 2010-04-09 2013-10-15 International Business Machines Corporation Nanopore capture system
EP2593568B1 (en) 2010-07-14 2017-04-19 The Curators Of The University Of Missouri Nanopore-facilitated single molecule detection of nucleic acids
EP2614156B1 (en) 2010-09-07 2018-08-01 The Regents of The University of California Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme
US9121059B2 (en) 2010-12-22 2015-09-01 Genia Technologies, Inc. Nanopore-based single molecule characterization
BR112013020411B1 (pt) * 2011-02-11 2021-09-08 Oxford Nanopore Technologies Limited Monômero de msp mutante, construto, polinucleotídeo, poro, kit e aparelho para caracterizar uma sequência de ácido nucleico alvo, e, método para caracterizar uma sequência de ácido nucleico alvo
WO2012113780A1 (en) * 2011-02-21 2012-08-30 Qiagen Gmbh Method for quantifying human dna
EP2689028B1 (en) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
SG10201604316WA (en) 2011-05-27 2016-07-28 Oxford Nanopore Tech Ltd Coupling method
WO2012178093A1 (en) * 2011-06-24 2012-12-27 Electronic Biosciences Inc. High contrast signal to noise ratio device components
KR20140050067A (ko) * 2011-07-25 2014-04-28 옥스포드 나노포어 테크놀로지즈 리미티드 막횡단 포어를 사용한 이중 가닥 폴리뉴클레오티드 서열분석을 위한 헤어핀 루프 방법
WO2013016486A1 (en) 2011-07-27 2013-01-31 The Board Of Trustees Of The University Of Illinois Nanopore sensors for biomolecular characterization
EP2769007B1 (en) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
CN104039979B (zh) * 2011-10-21 2016-08-24 牛津纳米孔技术公司 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法
CN102430153A (zh) * 2011-12-24 2012-05-02 中国人民解放军第三军医大学第二附属医院 一种基于交联技术的仿生骨界面、构建方法及其应用
CA2861808C (en) 2011-12-29 2021-02-23 Oxford Nanopore Technologies Limited Enzyme method
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
CA2864035C (en) 2012-02-15 2021-05-18 Oxford Nanopore Technologies Limited A method for determining the presence of an analyte using an aptamer
BR102012007246A2 (pt) 2012-03-30 2016-02-10 Fundação Antonio Prudente métodos para prognóstico e classificação de resultados de um evento
WO2013153359A1 (en) 2012-04-10 2013-10-17 Oxford Nanopore Technologies Limited Mutant lysenin pores
CA2879355C (en) 2012-07-19 2021-09-21 Oxford Nanopore Technologies Limited Helicase construct and its use in characterising polynucleotides
AU2013291763B2 (en) 2012-07-19 2019-06-20 Oxford Nanopore Technologies Limited Modified helicases
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
WO2014041337A1 (en) 2012-09-14 2014-03-20 Oxford Nanopore Technologies Limited Sample preparation method
WO2014072703A1 (en) 2012-11-06 2014-05-15 Oxford Nanopore Technologies Limited Quadruplex method
CA2901545C (en) 2013-03-08 2019-10-08 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
EP3575410A3 (en) 2013-10-18 2020-03-04 Oxford Nanopore Technologies Limited Modified enzymes
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
US10337060B2 (en) 2014-04-04 2019-07-02 Oxford Nanopore Technologies Ltd. Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
WO2016059436A1 (en) 2014-10-17 2016-04-21 Oxford Nanopore Technologies Limited Method for nanopore rna characterisation

Also Published As

Publication number Publication date
US20200063199A1 (en) 2020-02-27
WO2016059436A1 (en) 2016-04-21
US10480026B2 (en) 2019-11-19
CN107109489B (zh) 2021-11-30
EP3207155A1 (en) 2017-08-23
EP3207155B1 (en) 2019-06-05
US20250084472A1 (en) 2025-03-13
CN107109489A (zh) 2017-08-29
US12129518B2 (en) 2024-10-29
US11021747B2 (en) 2021-06-01
US20170253923A1 (en) 2017-09-07
US20230012471A9 (en) 2023-01-12
JP6824881B2 (ja) 2021-02-03
JP2017532962A (ja) 2017-11-09
US20210395811A1 (en) 2021-12-23
CN113981055A (zh) 2022-01-28
KR20170072915A (ko) 2017-06-27

Similar Documents

Publication Publication Date Title
US20250084472A1 (en) Method for nanopore rna characterisation
US12473593B2 (en) Method of characterizing a target ribonucleic acid (RNA) comprising forming a complementary polynucleotide which moves through a transmembrane pore
US20230250474A1 (en) Sample preparation method
EP2895618B1 (en) Sample preparation method
KR102280161B1 (ko) 방법
EP3259281B1 (en) Hetero-pores
EP3033435B1 (en) Method for fragmenting nucleic acid by means of transposase
JP6298404B2 (ja) 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
EP3519585A1 (en) Method for nucleic acid detection by guiding through a nanopore
WO2026057755A1 (en) Adaptors and kits for rna molecules labelling and characterising

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

R18-X000 Changes to party contact information recorded

St.27 status event code: A-3-3-R10-R18-oth-X000

A201 Request for examination
E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

PN2301 Change of applicant

St.27 status event code: A-3-3-R10-R13-asn-PN2301

St.27 status event code: A-3-3-R10-R11-asn-PN2301

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

St.27 status event code: A-1-2-D10-D22-exm-PE0701

GRNT Written decision to grant
PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U12-oth-PR1002

Fee payment year number: 1

PG1601 Publication of registration

St.27 status event code: A-4-4-Q10-Q13-nap-PG1601

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 4

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-OTH-PR1001 (AS PROVIDED BY THE NATIONAL OFFICE)

Year of fee payment: 4