CN107099572A - A kind of processing method of Optimization of Low Value Fish antibacterial peptide - Google Patents
A kind of processing method of Optimization of Low Value Fish antibacterial peptide Download PDFInfo
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- CN107099572A CN107099572A CN201710390345.6A CN201710390345A CN107099572A CN 107099572 A CN107099572 A CN 107099572A CN 201710390345 A CN201710390345 A CN 201710390345A CN 107099572 A CN107099572 A CN 107099572A
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- antibacterial peptide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/461—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from fish
Abstract
The invention discloses a kind of processing method of Optimization of Low Value Fish antibacterial peptide, comprise the following steps：Prepare and degerming, degreasing, microwave abstracting purification, centrifugation purification, the purifying of enzymolysis, centrifugal purification, ammonium sulfate and finished product, fat in Optimization of Low Value Fish air bladder carries out degreasing, filter out impurity and purify protein therein, protein is set to be hydrolyzed to antibacterial peptide by enzyme digestion reaction afterwards, increase the activity of compound protease using enzymatic activity peptide, accelerate hydrolysis, improve processing efficiency.Have the beneficial effect that：Antibacterial peptide is produced using Optimization of Low Value Fish air bladder, it is set to obtain higher value application, the features such as antibacterial peptide of the inventive method production has efficient natural, nontoxic, pollution-free, antibacterial, antibacterial spectrum width, last gained antibacterial peptide high income, purity are high, industrial production is may apply to, there is good development prospect.
Description
Technical field
The present invention relates to antibacterial peptide manufacture field, more particularly, to a kind of processing method of Optimization of Low Value Fish antibacterial peptide.
Technical background
Antibacterial peptide is the micromolecule polypeptide that a class has antibacterial action, is typically made up of 12-15 amino acid, antibacterial peptide because
Its excellent antibacterial characteristics reduces the incidence of disease of infectious disease, the ideal substitute as antibiotic, is sent out from nature at present
More than 2,000 kinds of antibacterial peptide is showed, antibacterial peptide has broad spectrum antibacterial, can kill gram-positive bacteria, Gram-negative bacteria and fungi
Deng.Antibacterial peptide has the advantages that to have no toxic side effect as antibacterials, noresidue, pollution-free, the preparation on antibacterial peptide at present
Deploy extensively with Antibacterial Mechanism research.One of acquisition methods of antibacterial peptide enzymatic isolation method is by hydrolyzing agricultural byproducts and aquatic products etc.
Discarded object obtains antibacterial peptide, is to produce one of most promising method of antibacterial peptide in enormous quantities, this method is in antibacterial peptide is prepared
It is widely applied.
Small fish, shrimp and disposal from fishery product processing such as internal organ in catches etc. be referred to as low value fishery -ies product, Optimization of Low Value Fish because
Technical reason uses to produce feed or even directly abandon directly as waste, and most Optimization of Low Value Fish contains abundant albumen
Matter, is had a great deal of practical meanings and economic value using Optimization of Low Value Fish enzymatic isolation method production antibacterial peptide.
The technology for preparing antibacterial peptide on enzymatic isolation method has many methods, and prior art is as authorized public number
The B of CN 103388014 Chinese invention patent, discloses a kind of ferrous chelating antibacterial peptide preparation method, and this method has
Simple to operate, with low cost the features such as, but it has the shortcomings that impurity is remained, antibacterial peptide amount to obtain is few, purity is not high.
The content of the invention
The purpose of this method is to provide a kind of processing method of Optimization of Low Value Fish antibacterial peptide.This method has materials environmental protection, former
Expect cheap, the impurity less residue such as bacterium, aliphatic acid, finished product acquisition rate is high, the advantages of purity is high.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：A kind of Optimization of Low Value Fish antibacterial peptide plus
Work method, including：Prepare and degerming, degreasing, microwave abstracting purification, centrifugation purification, enzymolysis, centrifugal purification, ammonium sulfate purify and
Finished product, specifically includes following steps：
Prepare and degerming：Take fresh air bladder some, split and remove blood vessel and mucous membrane after cleaned 3-5 times with distilled water, shred
20-30g distilled water can be added in closed rustless steel container by weighing 10-15g and being placed in 500ml, be 50-80MPa, temperature in pressure
For boiling 30-50 minutes under the conditions of 90-100 DEG C, its general principle sterilized was exactly lethal effect of the HTHP to microorganism,
Mainly realized by the activity for destroying cell membrane inhibitory enzyme and the duplication for influenceing the inhereditary materials such as DNA；
Degreasing：0.010-0.015g lipase is added in the air bladder slurries obtained after sterilization step, in 35-38 DEG C of condition
Under, stir and divulge information, it is reacted 20-30 minutes, then heating to 90-100 DEG C of boiling inactivates enzyme in 5 minutes, is down to normal temperature,
Fat enzymolysis and fatty acid oxidation are completed, degreasing air bladder slurries are obtained, fat enzymolysis and fatty acid oxidation can effectively reduce fat
The content of the metabolite of acid；
Microwave abstracting is purified：By in the air bladder slurries injection 1L beakers after defatting step, slurry weight is weighed, with distilled water not
Residual slurry on rust steel container wall is rinsed into beaker, and toward distilled water is added in beaker, until the steaming used of eluant container wall
Distilled water and the distilled water gross weight being directly added into beaker are 25-30 times of air bladder slurry weight, stir, slurries are carried out
Microwave abstracting, extraction power is 300-400W, and extraction time is 2-3 minutes, and microwave abstracting can be miscellaneous by what is generated in defatting step
Thing is removed, the effective accounting for improving protein in slurries；
Centrifugation purification：Extract after microwave abstracting purification step is centrifuged 15-20 minutes under the conditions of 4000-4500r/min,
Supernatant is taken, extraction is centrifuged again with distilled water rinse centrifuge tube and residue once, it is fish maw protein extract solution to merge supernatant,
Centrifugation purification further purifies fish maw protein matter；
Enzymolysis：It will centrifuge in the fish maw protein extract solution injection closed rustless steel containers of 500ml after purification, with clear water rinse beaker
Wall, rinse liquid is also injected into rustless steel container, and it is 5-7 to adjust its pH with 0.1ml hydrochloric acid, adds 0.07-0.1g compound egg
White enzyme, adds 0.02-0.05g enzymatic activity peptide, is reacted 2.5-3 hours at 50-60 DEG C, after be warming up to 90-100 DEG C of boiling 5
Minute inactivate enzyme, after be down to normal temperature, enzymatic activity peptide can effectively improve the hydrolysing activity of compound protease, improve hydrolysis speed
Rate, is antibacterial peptide by the effectively enzymolysis of the protein in extract solution, the amino acid sequence of the enzymatic activity peptide is：
SCASRCKSRCRARRCRARFGKRCICKCFRC；
Centrifugal purification：Enzymolysis liquid after enzymolysis step is placed in a centrifuge, in 35-38 DEG C under the conditions of 4000-4500r/min
Centrifugation 10-15 minutes, takes supernatant, by supernatant at 4-6 DEG C refrigerated centrifuge, under the conditions of the rotating speed 7500-8000r/min under
Centrifugation 10-15 minutes, takes supernatant standby, the content of antibacterial peptide effectively in purification enzymolysis liquid；
Ammonium sulfate is purified：Finely ground ammonium sulfate is added in above-mentioned supernatant according to 15-20% saturation degree, preserved at 4-6 DEG C
Centrifuged 30-45 minutes under 9500-10000r/min after overnight, take supernatant plus ammonium sulfate to 55-60% saturation degrees；At 4-6 DEG C
Centrifuged 30-45 minutes with 9500-10000r/min after preserving overnight, take precipitation to continue to employ, this step was effectively eliminated tentatively before this
The foreign protein of separation, rear precipitation gained is purifying antibacterial peptide.
Finished product：By ammonium sulfate after purification be deposited in -60 ~ -80 DEG C it is stored refrigerated, contribute to preserve transport.
Compared with prior art, the advantage of the invention is that：Air bladder used is low value fishery -ies product, discarded object air bladder is become useless
For treasured, higher value application is obtained, a kind of new approaches and methods is provided for the production of antibacterial peptide, is produced using the inventive method
Antibacterial peptide, belong to natural extract, be a kind of new green the features such as with natural, nontoxic, pollution-free, high-efficiency antimicrobial
Antimicrobial material, the enzymatic activity peptide added can effectively improve the hydrolysing activity of compound protease, improve hydrolysis reaction,
Last gained antibacterial peptide high income, purity are high, antibacterial spectrum width, there is good Industry Development Prospect.
Embodiment
This programme is described further below by embodiment：
Embodiment 1：
A kind of processing method of Optimization of Low Value Fish antibacterial peptide, including：Prepare and degerming, degreasing, microwave abstracting purification, centrifugation purification, enzyme
Solution, centrifugal purification, ammonium sulfate purifying and finished product, specifically include following steps：
1）Prepare and degerming：Take fresh air bladder some, split and remove blood vessel and mucous membrane after cleaned with distilled water 3 times, shred
25g distilled water can be added in closed rustless steel container by weighing 10g and being placed in 500ml, be 50MPa in pressure, temperature is 90 DEG C of conditions
Lower boiling 30 minutes；
2）Degreasing：0.010g lipase is added in the air bladder slurries obtained after step 1, under the conditions of 35 DEG C, stirs and leads to
Wind, makes it react 20 minutes, then heating to 90 DEG C of boilings inactivates enzyme in 5 minutes, is down to normal temperature, completes fat enzymolysis and fat
Acid oxidase, obtains degreasing air bladder slurries；
3）Microwave abstracting is purified：By in the air bladder slurries injection 1L beakers after step 2, slurry weight is weighed, with distilled water not
Residual slurry on rust steel container wall is rinsed into beaker, and toward distilled water is added in beaker, until the steaming used of eluant container wall
Distilled water and the distilled water gross weight being directly added into beaker are 25 times of air bladder slurry weight, stir, slurries are carried out micro-
Ripple is extracted, and microwave power is 300, and extraction time is 2 minutes；
4）Centrifugation purification：Extract after step 3 is centrifuged 15 minutes under the conditions of 4000r/min, supernatant is taken, distilled water is used
Rinse centrifuge tube and residue centrifuge extraction once again, and it is fish maw protein extract solution to merge supernatant；
5）Enzymolysis：Fish maw protein extract solution after step 4 is injected in the closed rustless steel containers of 500ml, with clear water rinse beaker
Wall, rinse liquid is also injected into rustless steel container, and it is 5 that its pH is adjusted with 0.1ml hydrochloric acid, adds 0.07g compound protease, plus
Enter 0.02g enzymatic activity peptides, reacted 2.5 hours at 50 DEG C, after be warming up to 90 DEG C of boilings and inactivate enzyme in 5 minutes, after be down to normal temperature；
6）Centrifugal purification：Enzymolysis liquid after step 5 is placed in a centrifuge, 10 points are centrifuged under the conditions of 4000r/min in 35 DEG C
Clock, takes supernatant, supernatant is lower under the conditions of refrigerated centrifuge, rotating speed 7500r/min at 4 DEG C to centrifuge 10 minutes, takes supernatant
It is standby；
7）Ammonium sulfate is purified：Finely ground ammonium sulfate is added in the supernatant obtained after step 6 according to 15% saturation degree, 4 DEG C
It is lower preserve overnight after centrifuged 30 minutes under 9500r/min, take supernatant plus ammonium sulfate to 55% saturation degree, preserve and stay overnight at 4 DEG C
Centrifuged 30 minutes with 9500r/min afterwards, take precipitation to continue to employ；
8）Finished product：By after step 7 be deposited in -60 DEG C it is stored refrigerated.
Embodiment 2：
A kind of processing method of Optimization of Low Value Fish antibacterial peptide, including：Prepare and degerming, degreasing, microwave abstracting purification, centrifugation purification, enzyme
Solution, centrifugal purification, ammonium sulfate purifying and finished product, specifically include following steps：
1）Prepare and degerming：Take fresh air bladder some, split and remove blood vessel and mucous membrane after cleaned with distilled water 5 times, shred
30g distilled water can be added in closed rustless steel container by weighing 15g and being placed in 500ml, be 80MPa in pressure, temperature is 100 DEG C of bars
Boiling 50 minutes under part；
2）Degreasing：0.015g lipase is added in the air bladder slurries obtained after step 1, under the conditions of 36 DEG C, stirs and leads to
Wind, makes it react 30 minutes, then heating to 100 DEG C of boilings inactivates enzyme in 5 minutes, is down to normal temperature, completes fat enzymolysis and fat
Fat acid oxidase, obtains degreasing air bladder slurries；
3）Microwave abstracting is purified：By in the air bladder slurries injection 1L beakers after step 2, slurry weight is weighed, with distilled water not
Residual slurry on rust steel container wall is rinsed into beaker, and toward distilled water is added in beaker, until the steaming used of eluant container wall
Distilled water and the distilled water gross weight being directly added into beaker are 30 times of air bladder slurry weight, stir, slurries are carried out micro-
Ripple is extracted, and microwave power is 400, and extraction time is 3 minutes；
4）Centrifugation purification：Extract after step 3 is centrifuged 20 minutes under the conditions of 4500r/min, supernatant is taken, distilled water is used
Rinse centrifuge tube and residue centrifuge extraction once again, and it is fish maw protein extract solution to merge supernatant；
5）Enzymolysis：Fish maw protein extract solution after step 4 is injected in the closed rustless steel containers of 500ml, with clear water rinse beaker
Wall, rinse liquid is also injected into rustless steel container, and it is 7 that its pH is adjusted with 0.1ml hydrochloric acid, adds 0.1g compound protease, plus
Enter 0.04g enzymatic activity peptides, reacted 3 hours at 60 DEG C, after be warming up to 100 DEG C of boilings and inactivate enzyme in 5 minutes, after be down to normal temperature；
6）Centrifugal purification：Enzymolysis liquid after step 5 is placed in a centrifuge, 15 points are centrifuged under the conditions of 4500r/min in 36 DEG C
Clock, takes supernatant, supernatant is lower under the conditions of refrigerated centrifuge, rotating speed 8000r/min at 6 DEG C to centrifuge 15 minutes, takes supernatant
It is standby；
7）Ammonium sulfate is purified：Finely ground ammonium sulfate is added in the supernatant obtained after step 6 according to 20% saturation degree, 5 DEG C
It is lower preserve overnight after centrifuged 45 minutes under 10000r/min, take supernatant plus ammonium sulfate to 55% saturation degree, preserved at 5 DEG C
Centrifuged 45 minutes with 10000r/min after night, take precipitation to continue to employ；
8）Finished product：By after step 7 be deposited in -80 DEG C it is stored refrigerated.
Embodiment 3：
A kind of processing method of Optimization of Low Value Fish antibacterial peptide, including：Prepare and degerming, degreasing, microwave abstracting purification, centrifugation purification, enzyme
Solution, centrifugal purification, ammonium sulfate purifying and finished product, specifically include following steps：
1）Prepare and degerming：Take fresh air bladder some, split and remove blood vessel and mucous membrane after cleaned with distilled water 5 times, shred
28g distilled water can be added in closed rustless steel container by weighing 12g and being placed in 500ml, be 70MPa in pressure, temperature is 95 DEG C of conditions
Lower boiling 40 minutes；
2）Degreasing：0.014g lipase is added in the air bladder slurries obtained after step 1, under the conditions of 37 DEG C, stirs and leads to
Wind, makes it react 25 minutes, then heating to 95 DEG C of boilings inactivates enzyme in 5 minutes, is down to normal temperature, completes fat enzymolysis and fat
Acid oxidase, obtains degreasing air bladder slurries；
3）Microwave abstracting is purified：By in the air bladder slurries injection 1L beakers after step 2, slurry weight is weighed, with distilled water not
Residual slurry on rust steel container wall is rinsed into beaker, and toward distilled water is added in beaker, until the steaming used of eluant container wall
Distilled water and the distilled water gross weight being directly added into beaker are 30 times of air bladder slurry weight, stir, slurries are carried out micro-
Ripple is extracted, and microwave power is 300, and extraction time is 2 minutes；
4）Centrifugation purification：Extract after step 3 is centrifuged 30 minutes under the conditions of 4000r/min, supernatant is taken, distilled water is used
Rinse centrifuge tube and residue centrifuge extraction once again, and it is fish maw protein extract solution to merge supernatant；
5）Enzymolysis：Fish maw protein extract solution after step 4 is injected in the closed rustless steel containers of 500ml, with clear water rinse beaker
Wall, rinse liquid is also injected into rustless steel container, and it is 6 that its pH is adjusted with 0.1ml hydrochloric acid, adds 0.08g compound protease, plus
Enter 0.03g enzymatic activity peptides, reacted 2.5 hours at 60 DEG C, after be warming up to 95 DEG C of boilings and inactivate enzyme in 5 minutes, after be down to normal temperature；
6）Centrifugal purification：Enzymolysis liquid after step 5 is placed in a centrifuge, 15 points are centrifuged under the conditions of 4000r/min in 37 DEG C
Clock, takes supernatant, supernatant is lower under the conditions of refrigerated centrifuge, rotating speed 8000r/min at 4 DEG C to centrifuge 15 minutes, takes supernatant
It is standby；
7）Ammonium sulfate is purified：Finely ground ammonium sulfate is added in the supernatant obtained after step 6 according to 20% saturation degree, 4 DEG C
It is lower preserve overnight after centrifuged 30 minutes under 10000r/min, take supernatant plus ammonium sulfate to 60% saturation degree, preserved at 4 DEG C
Centrifuged 30 minutes with 10000r/min after night, take precipitation to continue to employ；
8）Finished product：By after step 7 be deposited in -65 DEG C it is stored refrigerated.
By the antibacterial peptide obtained by embodiment 1-3 preparations with applying agar dilution as a control group with certain commercially available antibacterial peptide
Bacteriostatic experiment is carried out, easy steps are：By the antibacterial peptide of various dose, it is added to and melts and be cooled to 50 DEG C or so of quantitative agar
In, the agar plate containing different decreasing concentration antibacterial peptides is made, is inoculated with tested bacterium, bacterial growth situation is regularly observed after incubation.It is real
Test data such as following table：
Fungistatic effect after the antibacterial peptide of table 1. effect different time（%）
Obtained antibacterial peptide has broad spectrum antibacterial as can be seen from Table 1, and fungistatic effect is substantially better than commercial antimicrobial peptide, enzyme activity
Property peptide can effectively improve the reactivity of compound protease, accelerate its enzymolysis protein matter, the antibacterial peptide obtained by this method is natural,
It is nontoxic, pollution-free, therefore this method is a kind of processing method of efficient Optimization of Low Value Fish antibacterial peptide.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Zhoushan Da Kang Science and Technology Ltd.s
<120>A kind of processing method of Optimization of Low Value Fish antibacterial peptide
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 30
<212> PRT
<213>It is artificial synthesized
<400> 1
Ser Cys Ala Ser Arg Cys Lys Ser Arg Cys Arg Ala Arg Arg Cys Arg
1 5 10 15
Ala Arg Phe Gly Lys Arg Cys Ile Cys Lys Cys Phe Arg Cys
20 25 30
Claims (10)
1. a kind of processing method of Optimization of Low Value Fish antibacterial peptide, it is characterised in that comprise the following steps：
1）Prepare and degerming；
2）Degreasing：Lipase is added in the air bladder slurries obtained after step 1 and carries out degreasing；
3）Microwave abstracting is purified：Microwave abstracting is carried out to the degreasing slurries obtained after step 2；
4）Centrifugation purification：Centrifugation purification is carried out to the purification slurries obtained after step 3；
5）Enzymolysis：Compound protease is added in the purification slurries obtained after step 4 to be digested with enzymatic activity peptide；
6）Centrifugal purification：Multiple centrifugal purification is carried out to the enzymolysis liquid obtained after step 5；
7）Ammonium sulfate is purified：Ammonium sulfate is added in the mixed serum obtained after step 6 purify obtaining antibacterial peptide；
8）Finished product：The sterling antibacterial peptide obtained after step 7 is carried out stored refrigerated.
2. a kind of processing method of Optimization of Low Value Fish antibacterial peptide according to claim 1, it is characterised in that：In described step 1
Air bladder wash number is 3-5 times, takes weight for 10-15g, adds distilled water weight for 20-30g, and boiling pressure is 50-
80MPa, temperature is 90-100 DEG C, and digestion time is 30-50 minutes.
3. a kind of processing method of Optimization of Low Value Fish antibacterial peptide according to claim 1, it is characterised in that：In described step 5
The amino acid sequence of the enzymatic activity peptide added is：SCASRCKSRCRARRCRARFGKRCICKCFRC.
4. a kind of processing method of Optimization of Low Value Fish antibacterial peptide according to claim 1, it is characterised in that：In described step 2
The lipase weight of addition is 0.010-0.015g, is reacted 20-30 minutes under the conditions of 35-38 DEG C, then heats to 90-100
DEG C inactivate enzyme.
5. a kind of processing method of Optimization of Low Value Fish antibacterial peptide according to claim 1, it is characterised in that：In described step 3
Added distilled water gross weight is 25-30 times of air bladder slurry weight, and the microwave power of microwave abstracting is 300-400W, during extraction
Between be 2-3 minutes.
6. a kind of processing method of Optimization of Low Value Fish antibacterial peptide according to claim 1, it is characterised in that：In described step 4
The centrifuge speed of centrifugation purification is 4000-4500r/min, and centrifugation time is 15-20 minutes.
7. a kind of processing method of Optimization of Low Value Fish antibacterial peptide according to claim 1, it is characterised in that：In described step 5
The pH value of enzymolysis is 5-7, and the compound protease weight of addition is 0.07-0.1g, and the weight of the enzymatic activity peptide of addition is 0.02-
0.05g, hydrolysis temperature is 50-60 DEG C, and enzymolysis time is 2.5-3 hours, after be warming up to 90-100 DEG C and inactivate enzyme.
8. a kind of processing method of Optimization of Low Value Fish antibacterial peptide according to claim 1, it is characterised in that：In described step 6
In 35-38 DEG C under the conditions of 4000-4500r/min centrifugation time be 10-15 minutes, take supernatant in 4-6 DEG C in 7500-
Centrifugation time is 10-15 minutes under the conditions of 8000r/min.
9. a kind of processing method of Optimization of Low Value Fish antibacterial peptide according to claim 1, it is characterised in that：In described step 7
Finely ground ammonium sulfate is added in gained supernatant 15-20% saturation degree solution is made, in 4-6 DEG C in 9500-10000 DEG C of condition
Lower centrifugation 30-45 minutes；Take supernatant to add ammonium sulfate and 55-66% saturation degree solution be made, in -6 DEG C in 9500-10000
Centrifuged 30-45 minutes under the conditions of DEG C.
10. a kind of processing method of Optimization of Low Value Fish antibacterial peptide according to claim 1, it is characterised in that：In described step 8
Stored refrigerated temperature is -60--80 DEG C.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107568409A (en) * | 2017-09-23 | 2018-01-12 | 赵德润 | A kind of preparation method of colored glue active peptide |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1309345A2 (en) * | 2000-08-17 | 2003-05-14 | Cistem Biotechnologies GmbH | Cathelicidins as vaccine adjuvants |
| CN102051398A (en) * | 2009-10-29 | 2011-05-11 | 浙江海洋学院 | Method for preparing half-fin anchovy antibacterial peptides |
-
2017
- 2017-05-27 CN CN201710390345.6A patent/CN107099572A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1309345A2 (en) * | 2000-08-17 | 2003-05-14 | Cistem Biotechnologies GmbH | Cathelicidins as vaccine adjuvants |
| CN102051398A (en) * | 2009-10-29 | 2011-05-11 | 浙江海洋学院 | Method for preparing half-fin anchovy antibacterial peptides |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107568409A (en) * | 2017-09-23 | 2018-01-12 | 赵德润 | A kind of preparation method of colored glue active peptide |
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Application publication date: 20170829 |