CN107096062A - A kind of preparation method and application of the dressing for skin containing carragheen/agarose - Google Patents
A kind of preparation method and application of the dressing for skin containing carragheen/agarose Download PDFInfo
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- CN107096062A CN107096062A CN201710169113.8A CN201710169113A CN107096062A CN 107096062 A CN107096062 A CN 107096062A CN 201710169113 A CN201710169113 A CN 201710169113A CN 107096062 A CN107096062 A CN 107096062A
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- carragheen
- agarose
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/425—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/225—Mixtures of macromolecular compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/26—Mixtures of macromolecular compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
- A61L2300/408—Virucides, spermicides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
Abstract
The invention discloses a kind of preparation method and application of the dressing for skin containing carragheen/agarose.Described skin wound dressing is using mass ratio as (10 by carragheen and agarose:90)~(50:50) laminated film is obtained by convection drying after being blended, or freeze-drying obtains the compound rest with three-dimensional porous layer structure；Described carragheen is carragheen of the commerical grade carragheen after purification processes.The carragheen method of purification that the present invention is used solves the problem of carragheen differs greatly between commerical grade carragheen purity deficiency, each manufacturer, it is prepared the high biomaterial of stability.Dressing for skin obtained by the present invention can play organization healing and provide certain nutrition; and protect the surface of a wound, resistance virus to prevent the discovery of infection during organization healing; vivo immuning system partial function can be adjusted in the process simultaneously, veritably play the effect for promoting the surface of a wound to recover healing.
Description
Technical field
The invention belongs to biomedical materials field, and in particular to a kind of dressing for skin containing carragheen/agarose
Preparation method and application.
Background technology
Carragheen (Carrageenan), also known as antler glue, are that one kind is extracted in marine red alga and gone out, with linear
The anion polysaccharide that sulphation galactan is polymerized.Since being found from 20th century by scientist, carragheen is widely used
It is used in systematism processing, thickener and as gel-forming component among food and medicine industry.By years development, card
Glue is drawn already to turn into one of big algin in the world three.According to the difference in its structure, current commercialized carragheen is mainly κ
Type, ι types, three kinds of λ types, wherein with the carrageenan sulfated degree of esterification highest of λ types.Early in 1972, U.S.'s food and drug surveilance
Management board (Food And Drugs Administration) divides carragheen group in the classification of " Generally Recognized as safe ", meanwhile,
Because carragheen the separation of pre- grease-proof and can provide the property of nutrition, FDA also allows it to be applied to the production such as baby's dairy produce
Among product.In 2001, FAO (Food and Agriculture Organization of the United Nation) and the additive Committee of Experts of the World Health Organization eliminated and are directed to OK a karaoke club
The limitation of glue acceptable daily intake, it is a kind of safe and nontoxic, addition for having no side effect further to confirm carragheen
Agent.Part carragheen has excellent gel-forming property, and such as κ carragheens itself have gel characteristic, and have in the solution micro-
The K of amount+In the presence of ion, gel strength will be greatly promoted, and be conducive to the application in food service industry.Except this it
Outside, its many potential medical value that carragheen is also exhibited, make it also gradually obtain widely should among pharmaceuticals industry
With.In gradually in-depth study, carragheen to it is many virus (such as：Herpesviral, inhibition of HIV etc.) there is the inhibitory activity of wide spectrum
Effect.Especially λ carragheens can by adsorption process of the blocking virus to host cell so that suppress herpesviral (HSV-1,
Infecting HSV-2), its activity is relevant with sulfur content.In addition carragheen is in terms of antitumor, antiviral, reducing blood lipid, anti-agglomeration
On have good effect, also have to the regulation of immune system and have certain effect.It can belong to natural many due to carragheen itself
The characteristic of sugar, its species is various.Obtained carragheen is extracted in marine red alga not of the same race in nature in composition and sulphur
It is different the index such as Esterification, or even also can in the carragheen property that marine red alga of the same race is obtained by distinct methods extraction
Difference.Commercially, different company is prepared and the commerical grade carragheen sold component with it is otherwise varied in purity,
Carragheen not of the same race may be present in certain single commerical grade carragheen simultaneously.Card is limited in these characteristic parts
Glue is drawn to need the application and development of the technical field of biological material of higher stability.
Agarose is a kind of linear polysaccharide for proposing to obtain among tangleweed, and it has hydrophily, electroneutral, chemistry
The properties such as stability and abundance, it is with low cost, food service industry has been widely used to since self-discovery.Agarose dissolves in 90
DEG C hot water formation solution, when its aqueous solution drops to 35 DEG C or so, never random coil gradually becomes list to agarose molecules
Chain or double-stranded helical coil conformation, helical conformation connect the tridimensional network of formation rule arrangement with flexible chain, finally
Make agarose solution gelation.Compared with other gel rubber materials, Ago-Gel has suitable advantage in mechanical performance
(such as：Viscoplasticity, compressibility etc.) and have more superior biocompatibility, a degradability.In view of above advantageous property, agarose should
Research for field of tissue engineering technology has been carried out the quite a while, and in terms of nerve, cartilage, mucous membrane of mouth, cornea
Achieve some gratifying achievements in research.But because special anti-of Ago-Gel is adhered characteristic, it is not used alone, need
With other materials it is used in conjunction with that composite is made improving the cell adhesion of obtained material.Current Ago-Gel exists
Sufficiently studied and applied in the fields such as cartilage filling renovation material, bone impairment renovation material, injured nerve protection materials.
Skin, is the maximum organ of human body, accounts for 16% or so of normal person's body weight.As the most key between human body and environment
Physical barriers, skin undertake this it is many maintain human normals operation important physiological functions.Control section material (water, electrolysis
Matter etc.) human body is passed in and out, stops various types of radiation, completely cut off the part that extraneous harmful substance is this variety of important physiological function.Can
As the first line of defence of human body, when wound occurs, skin is then to stand in the breach to sustain damage.In view of skin injury is universal
Property, the research for the dressing of skin repair is also valued by people.Skin repair is divided into four-stage：1) hemostasis, 2) inflammation
Disease, 3) propagation, 4) remodeling.Each stage is directed to, different requirements are also generated in the performance to dressing for skin.It is preferable
Dressing for skin should have：1) contribute to blood coagulation, prevent wound Continuous hemorrhage from deteriorating；2) protect the surface of a wound ventilative while not by the external world
Harmful substance infects；3) dressing mitigates pain and does not glue wound when using, it is to avoid secondary insult is caused during taking-up；4) it is easy to make
With, be not required to frequently replacing.
In existing market in the prior art, existing many casting products：1) collagen/nylon；2) collagen/agar；3) silk egg
In vain；4) acellular dermal matrix；5) other natural or artificial synthesized macromolecules etc..These products are to be based on collagen/sulphur mostly
The materials such as aching and limp ossein, collagen/nylon, collagen/silicon rubber, existing market occupation rate is low, using less.
In existing skin ultrastructure material, because of carragheen own partial characteristic, seldom make to itself and its derivative
For raw material and related component application.But carragheen is nontoxic and with good gelling performance, has partial monopoly to be related to OK a karaoke club
Glue is as the gelling agent of the materials such as capsule, jelly, and such as patent CN201510654564.1 is by carrageenan treatment as plant flexible glue
Capsule material；κ, ι type carrageenan treatment are used for non-gelatin capsules shell etc. by patent CN03806246.1.
The content of the invention
In order to solve the deficiency and technical problem of existing dressing for skin, and commerical grade carragheen is being asked present on application
Topic, primary and foremost purpose of the invention is to provide a kind of dressing for skin containing carragheen/agarose.The dressing can be organization healing
Certain nutrition is provided, and protects the surface of a wound, resistance virus to prevent the discovery of infection during organization healing, while in the process
Vivo immuning system partial function can be adjusted, veritably plays a part of promoting the surface of a wound to recover healing.
Another object of the present invention is to provide the preparation method of the above-mentioned dressing for skin containing carragheen/agarose.Should
The commerical grade carragheen that method effectively solves different company is used to causing property when prepared by biomaterial that difference to be asked
Topic, makes obtained timbering material stability be significantly improved.
A further object of this patent is the application for providing the above-mentioned dressing for skin containing carragheen/agarose.
In order to realize foregoing invention, the present invention takes following technical scheme to implement：
A kind of dressing for skin containing carragheen/agarose, described dressing for skin be by carragheen and agarose with
(10:90)~(50:50) after mass ratio blending, be cooled into gel has three-dimensional porous layer by what freeze-drying was obtained again
The laminated film that convection drying is obtained after the compound support frame material of shape structure, or blending；Described carragheen is commerical grade card
Draw carragheen of the glue after purification processes.
The skin extra earning repair materials of the present invention can play organization healing and provide certain nutrition, and in organization healing
The period protection surface of a wound, resistance virus prevent the discovery of infection, while can adjust vivo immuning system partial function in the process, inhale
Draw neutrophil leucocyte and reach impaired place, accelerating wound healing veritably plays the effect for promoting the surface of a wound to recover healing.
The purification processes step is：Preparation mass fraction is 10% commerical grade carrageenan solutions, is placed in 90 DEG C of water-baths
Dissolve by heating, filter while hot；Add same volume 0.8mol/L KCl solution；Stand or centrifuge at room temperature, take precipitation to be placed in
Dialysed three days in bag filter, concentrated by rotary evaporation；Stay overnight, be freeze-dried in -20 DEG C of deep colling.
Described carragheen is that commerical grade carragheen passes through purification processes, improves required compositional purity and removes deimpurity card
Draw glue.
It is preferred that, the carragheen includes one kind in κ types carragheen, ι types carragheen, λ type carragheens.
It is preferred that, described three-dimensional porous layer structure compound rest thickness is 3~3.5 millimeters.
It is preferred that, described laminated film thickness is 2~2.5 millimeters.
The above-mentioned dressing for skin containing carragheen/agarose is prepared using following steps：
(1) it is 10% commerical grade carrageenan solutions to prepare mass fraction, is placed in 90 DEG C of water-baths and dissolves by heating, while hot mistake
Filter；Add same volume 0.8mol/L KCl solution；Stand or centrifuge at room temperature, take precipitation to be placed in bag filter and dialyse three
My god, concentrated by rotary evaporation；Stay overnight, be freeze-dried in -20 DEG C of deep colling, it is standby；
(2) with the pure water dissolving agarose for being heated to 75~90 DEG C, be stirred continuously, obtain uniform, mass concentration be 1~
2% agarose solution；
(3) carragheen for purifying step (1) is added in step (2) in the agarose solution of gained, and temperature is maintained at
75~90 DEG C, during which stir and evenly mix, obtain carragheen/agarose blend solution, the mass ratio of wherein carragheen/agarose is
(10:90)~(50:50)；
(4) blend solution obtained by step (3) is poured into convection drying in mould, obtains laminated film；Or it is molten mixing
Liquid is poured into mould, is placed under -20 DEG C of environment and is freezed freeze-drying after 4~5h, obtains answering with three-dimensional porous layer structure
Close timbering material.
Step (1) described carragheen preferably includes one kind among κ types carragheen, ι types carragheen, λ type carragheens.
When the carragheen is commerical grade kappa-carrageenan, specific purification step is as follows：
Preparation mass fraction is 10% commerical grade kappa-carrageenan solution, is placed in 90 DEG C of water-baths and dissolves by heating, filters while hot；
Add same volume 0.8mol/L KCl solution；Stand at room temperature, abandoning supernatant is decanted；Insert and dialyse three in bag filter
My god, concentrated by rotary evaporation；- 20 DEG C of deep colling are stayed overnight, freeze-drying, standby.
When the carragheen is commerical grade ι-carragheen, specific purification step is as follows：
Preparation mass fraction is 10% ι-carrageenan solutions, is placed in 90 DEG C of water-baths and is heated to being completely dissolved, filters while hot；
Add same volume 0.8mol/L KCl solution；High speed centrifugation, abandoning supernatant；Taking precipitate is dialysed three days in bag filter,
Concentrated by rotary evaporation；- 20 DEG C of deep colling are stayed overnight, freeze-drying, standby.The ι-carragheen purification step high speed centrifugal condition is
8000rpm, 20min.
When the carragheen is commerical grade lambda-carrageenan, specific purification step is as follows：
Preparation mass fraction is 10% lambda-carrageenan solution, is placed in 90 DEG C of water-baths and is heated to being completely dissolved, filters while hot；
Add same volume 0.8mol/L KCl solution；High speed centrifugation, takes supernatant in clean beaker；Add two volumes anhydrous
Ethanol, decantation；Take precipitation to be placed in bag filter to dialyse three days, concentrated by rotary evaporation；- 20 DEG C of deep colling are stayed overnight, freeze-drying, standby.Institute
Lambda-carrageenan purification step high speed centrifugal condition is stated for 8000rpm, 10min.
It is preferred that, the bag filter molecular cut off used in being dialysed described in step (1) is 3000.
It is preferred that, mixing speed described in step (2) is 600r/min.
It is preferred that, stir to stir untill mixing is completely with 600r/min at 75~90 DEG C described in step (3).
It is preferred that, the convection drying described in step (4) is that mould is placed at 20~35 DEG C to dry 48~72h, gained
Dressing for skin containing carragheen/agarose is laminated film.
It is preferred that, the freeze-drying described in step (4) is to be freezed at -20~-30 DEG C after 4~5h, in freeze-drying
Machine cold-trap is -30~-60 DEG C, is evacuated in the state of vacuum and dries 36~48h, the skin containing carragheen/agarose of gained
Dressing is the compound support frame material with three-dimensional porous layer structure.
The above-mentioned dressing for skin containing carragheen/agarose can be answered in dressing for skin and tissue engineering bracket material field
With.
Room temperature of the present invention refers to 0~30 DEG C.
Compared with prior art, the present invention has advantages below and beneficial effect：
(1) dressing for skin provided by the present invention containing carragheen/agarose can provide nutrient energy to wound site,
And because of carragheen special property, during use the surface of a wound can be protected not to be infected by the virus, maintain normal healing process.
(2) commerical grade carragheen method of purification provided by the present invention can separate different types of carragheen, needed for making
Specific carragheen content be greatly improved so that steady using the dressing for skin of carragheen/agarose obtained by it
Qualitative stronger, every property is consistent.
(3) extensively, manufacturing cost is low for the dressing for skin raw material sources provided by the present invention containing carragheen/agarose,
And it is strong in improving raw material added value, (carragheen, agarose are all by large-scale for the development of the marine alga plantation industry of promotion surrounding area
Extracted in marine alga).
(4) dressing for skin provided by the present invention containing carragheen/agarose is easy to use, and with good moistening
Property, adhesion will not be produced to the surface of a wound, the generation of secondary insult when preventing from taking out.
(5) dressing for skin provided by the present invention containing carragheen/agarose has a wide range of application, and can be applied to burn,
In the large area skin wound repair such as skin ulcer.And relative to the existing dressing such as agarose/collagen, in stability and intensity
Increase, dressing of the invention is in value of the biomedical materials field with more application.
Brief description of the drawings
Fig. 1 is obtained κ types carragheen/agarose compound rest cross section SEM figures made from embodiment 2.
Fig. 2 is ι types carragheen/agarose compound rest cross section SEM figures obtained by embodiment 3.
Embodiment
With reference to embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited
In this.
Embodiment 1
A kind of dressing for skin laminated film containing λ types carragheen/agarose, be by λ types carragheen and agarose according to
50:The laminated film that 50 mass ratio is constituted.
The preparation method of the above-mentioned dressing for skin laminated film containing λ types carragheen/agarose comprises the following steps：
(1) it is 10% commerical grade lambda-carrageenan solution to prepare mass fraction, is placed in 90 DEG C of water-baths and is heated to being completely dissolved,
Filter while hot.Add same volume 0.8mol/L KCl solution.50mL centrifuge tubes high speed centrifugation (8000rpm, 10min) is inserted,
Supernatant is taken in clean beaker.Add two volumes absolute ethyl alcohol, decantation.Precipitation is taken to be placed in the dialysis of molecular cut off 3000
Dialysed three days in bag, concentrated by rotary evaporation.It is placed in deep colling in -20 DEG C of refrigerators to stay overnight, is freeze-dried.
(2) agarose is dissolved with 90 DEG C of pure water, obtains the agarose solution that mass fraction is 1%.
(3) the λ type carragheens purified are added in above-mentioned agarose solution, and in 80 DEG C, 600r/min condition
Under be stirred continuously make its be well mixed, solution bubble-free obtains carragheen/agarose blend solution.Wherein carragheen and agar
The mass ratio of sugar is 50:50.
(4) blend solution obtained by step (3) is poured into mould and cooled down, the height of blend solution in a mold is about 3 millis
λ types carragheen/agarose laminated film is made in rice, convection drying 36h under the conditions of mould then is placed in into 20~35 DEG C.
The lambda-carrageenan in step (1) before and after purification is taken to carry out elementary analysis, as a result as shown in table 1, λ-OK a karaoke club after purification
Glue content is greatly improved.
The lambda-carrageenan elementary analysis result of table 1
| Lambda-carrageenan | Sulfur content (%) | Sulfate group content (%) |
| Before purification | 7.20 | 18.00 |
| After purification | 9.75 | 24.38 |
Obtained λ types carragheen/agarose laminated film thickness is about 2~2.5 millimeters, Co 60 illumination-based disinfection standby
With.
Embodiment 2
A kind of dressing for skin compound rest containing κ types carragheen/agarose, be by κ types carragheen and agarose according to
40:The compound support frame material that 60 mass ratio is constituted, stretch modulus 0.292MPa.
The preparation method of the above-mentioned dressing for skin compound rest containing κ types carragheen/agarose, comprises the following steps：
(1) it is 10% commerical grade kappa-carrageenan solution to prepare mass fraction, is placed in 90 DEG C of water-baths and dissolves by heating, while hot mistake
Filter.Add same volume 0.8mol/L KCl solution.Stand at room temperature, abandoning supernatant is decanted.Inserting molecular cut off is
Dialysed three days in 3000 bag filter, concentrated by rotary evaporation.Insert deep colling in -20 DEG C of refrigerators to stay overnight, be freeze-dried.
(2) agarose is dissolved with 90 DEG C of pure water, obtains the agarose solution that mass fraction is 1%.
(3) the κ type carragheens purified are added in above-mentioned agarose solution, and in 80 DEG C, 600r/min condition
Under be stirred continuously make its be well mixed, solution bubble-free obtains carragheen/agarose blend solution.Wherein carragheen and agar
The mass ratio of sugar is 40:60.
(4) blend solution obtained by step (3) is poured into mould and cooled down, the height of blend solution in a mold is about 4 millis
Rice, is then placed in 4~5h of freezing at -20 DEG C by mould.The mould of deep colling is put into freeze drier, condenser temperature for-
30~-60 DEG C, pumping freeze-drying 36h under conditions of vacuum, are made κ types carragheen/agarose compound rest.
Obtained κ types carragheen/agarose compound rest thickness is about 3~3.5 millimeters, Co 60 illumination-based disinfection standby
With.
Electron microscopic observation is scanned to obtained κ types carragheen/agarose compound rest cross section, its cross-sectional structure
As shown in Figure 1.
Embodiment 3
A kind of dressing for skin compound rest containing ι types carragheen/agarose, be by ι types carragheen and agarose according to
50:The compound support frame material that 50 mass ratio is constituted, stretch modulus 0.321MPa.
The preparation method of the above-mentioned dressing for skin compound rest containing ι types carragheen/agarose, comprises the following steps：
(1) it is 10% commerical grade ι-carrageenan solutions to prepare mass fraction, is placed in 90 DEG C of water-baths and is heated to being completely dissolved,
Filter while hot.Add same volume 0.8mol/L KCl solution.50mL centrifuge tubes high speed centrifugation (8000rpm, 20min) is inserted,
Abandoning supernatant.Taking precipitate is dialysed three days in molecular cut off is 3000 bag filter, concentrated by rotary evaporation.Insert -20 DEG C of ice
Deep colling is stayed overnight in case, freeze-drying.
(2) agarose is dissolved with 90 DEG C of pure water, obtains the agarose solution that mass fraction is 1%.
(3) the ι type carragheens purified are added in above-mentioned agarose solution, and under 90 DEG C, 600r/min constantly
Stirring is well mixed it, and solution bubble-free obtains carragheen/agarose blend solution.The wherein matter of carragheen and agarose
Amount is than being 50:50.
(4) blend solution obtained by step (3) is poured into mould and cooled down, the height of blend solution in a mold is about 4 millis
Rice, is then placed in 4~5h of freezing at -20 DEG C by mould.The mould of deep colling is put into freeze drier, condenser temperature for-
30~-60 DEG C, pumping freeze-drying 36h under conditions of vacuum, are made ι types carragheen/agarose compound rest.
Obtained ι types carragheen/agarose compound rest thickness is about 3~3.5 millimeters, Co 60 illumination-based disinfection standby
With.
Electron microscopic observation is scanned to obtained ι types carragheen/agarose compound rest cross section, its cross-sectional structure
As shown in Figure 2.
ι types carragheen/effect of the agarose compound rest in 3T3 cytotoxicity experiments：In 37 DEG C, 5%CO2Condition
Under ι types carragheen/agarose compound rest 24h leaching liquors are prepared with 0.1g/mL concentration, gained leaching liquor is added to and carried
The good 3T3 cell densities of preceding kind are 1*10572h is cultivated in 24 orifice plates in individual/hole.Measured after 72h experimental group cell survivaling number with it is right
It is experimental group 98.7% according to a group no significant difference, it was demonstrated that gained ι types carragheen/agarose compound rest safety, acellular poison
Property.
Above-described embodiment is preferably embodiment, but embodiments of the present invention are not by above-described embodiment of the invention
Limitation, other any Spirit Essences without departing from the present invention and the change made under principle, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (10)
1. a kind of dressing for skin containing carragheen/agarose, it is characterised in that described dressing for skin is by carragheen and fine jade
Lipolysaccharide is with (10:90)~(50:50) after mass ratio blending, be cooled into gel has three-dimensional by what freeze-drying was obtained again
The laminated film that convection drying is obtained after the compound support frame material of porous laminated structure, or blending；Described carragheen is business
Carragheen of the grade carragheen after purification processes.
2. a kind of dressing for skin containing carragheen/agarose according to claim 1, it is characterised in that the purification
Process step is：Preparation mass fraction is 10% commerical grade carrageenan solutions, is placed in 90 DEG C of water-baths and dissolves by heating, while hot mistake
Filter；Add same volume 0.8mol/L KCl solution；Stand or centrifuge at room temperature, take precipitation to be placed in bag filter and dialyse three
My god, concentrated by rotary evaporation；Stay overnight, be freeze-dried in -20 DEG C of deep colling.
3. a kind of dressing for skin containing carragheen/agarose according to claim 1, it is characterised in that the OK a karaoke club
Glue includes one kind in κ types carragheen, ι types carragheen or λ type carragheens.
4. a kind of dressing for skin containing carragheen/agarose according to claim 1, it is characterised in that described tool
The compound support frame material thickness for having three-dimensional porous layer structure is 3~3.5 millimeters.
5. a kind of dressing for skin containing carragheen/agarose according to claim 1, it is characterised in that described answers
It is 2~2.5 millimeters to close film thickness.
6. a kind of dressing for skin containing carragheen/agarose, it is characterised in that comprise the following steps：
(1) it is 10% commerical grade carrageenan solutions to prepare mass fraction, is placed in 90 DEG C of water-baths and dissolves by heating, filters while hot；Plus
Enter same volume 0.8mol/L KCl solution；Stand or centrifuge at room temperature, take precipitation to be placed in bag filter and dialyse three days, rotation
Inspissation contracts；Stay overnight, be freeze-dried in -20 DEG C of deep colling, it is standby；
(2) with the pure water dissolving agarose for being heated to 75~90 DEG C, stirring obtains the agarose that mass concentration is 1~2% molten
Liquid；
(3) carragheen for purifying step (1) is added in step (2) in the agarose solution of gained, and temperature is maintained at 75~
90 DEG C, stir and evenly mix, obtain carragheen/agarose blend solution, the wherein mass ratio of carragheen and agarose is (10:90)~
(50:50)；
(4) blend solution obtained by step (3) is poured into convection drying in mould, obtains laminated film；Or blend solution is fallen
Enter in mould, be placed under -20 DEG C of environment and freeze freeze-drying after 4~5h, obtain the composite support with three-dimensional porous layer structure
Frame material.
7. a kind of dressing for skin containing carragheen/agarose according to claim 6, it is characterised in that step (1) institute
State carragheen and preferably include one kind among κ types carragheen, ι types carragheen or λ type carragheens；
The bag filter molecular cut off used in being dialysed described in step (1) is 3000.
8. a kind of dressing for skin containing carragheen/agarose according to claim 6, it is characterised in that step (2),
(3) mixing speed described in is 600r/min.
9. a kind of dressing for skin containing carragheen/agarose according to claim 6, it is characterised in that, step (4)
Described in convection drying be by mould be placed at 20~35 DEG C dry 48~72h, skin of the gained containing carragheen/agarose
Dressing is laminated film；
Freeze-drying described in step (4) be at -20~-30 DEG C freeze 4~5h after, in freeze drier cold-trap be -30
~-60 DEG C, be evacuated in the state of vacuum and dry 36~48h, the dressing for skin containing carragheen/agarose of gained is with three
Tie up the compound support frame material of porous laminated structure.
10. the dressing for skin containing carragheen/agarose described in any one of claim 1 to 5 is in dressing for skin and tissue work
Application in engineering support Material Field.
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| CN108578761A (en) * | 2018-04-12 | 2018-09-28 | 西南大学 | Load the preparation method and applications of silk gum/Ago-Gel of lysozyme |
| CN113425644A (en) * | 2021-07-16 | 2021-09-24 | 山东大学 | Rose mask and preparation method and application thereof |
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| CN113425644A (en) * | 2021-07-16 | 2021-09-24 | 山东大学 | Rose mask and preparation method and application thereof |
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