CN107094497A - A kind of Cordyceps militaris aerial hyphae sweeps bacterium method - Google Patents
A kind of Cordyceps militaris aerial hyphae sweeps bacterium method Download PDFInfo
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- CN107094497A CN107094497A CN201710360079.2A CN201710360079A CN107094497A CN 107094497 A CN107094497 A CN 107094497A CN 201710360079 A CN201710360079 A CN 201710360079A CN 107094497 A CN107094497 A CN 107094497A
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Abstract
Bacterium method is swept the present invention relates to a kind of Cordyceps militaris aerial hyphae, this method includes：Cordyceps militaris link bacterial strain is cultivated in magazine to cover with the snow-white mycelia of fish scale-shaped and annesl to yellow but without producing the fructification young shoot stage, the aerial hyphae on substrate mycelia is all swept in an aseptic environment using the wooden hair planting brush of old mycelia remover tool, to stimulate substrate mycelia to form former base budding as early as possible.Present invention reduces the time of cordycep militaris fruiting body entity primodium formation, and Cordyceps militaris former base is set to break up neat and consistent, so as to shorten the cultivation cycle of Cordyceps militaris, budding is neat, condition is good, improves the quality and yield of Cordyceps militaris.
Description
Technical field
The present invention relates to the technical field of Applied Biotechnology principle artificial culture fungi, and in particular to the training of Cordyceps militaris
Educate, relate more specifically to a kind of Cordyceps militaris aerial hyphae sweeps bacterium method.
Background technology
Cordyceps militaris also known as northern Chinese caterpillar Fungus, Cordceps militaris, pupa grass, northeast cordyceps sinensis, colonize in Lepidoptera, coleoptera, Diptera
Deng on insect pupal cell, warm nature in bacterium property category is mainly distributed on the provinces and regions such as northeast, North China, northwest.Fungi could is referred to as Cordyceps,
Belong to Ascomycetes Hypocreales Clavicipitaceae.Cordyceps militaris generally receives as Cordyceps, more domestic and foreign scholars.Cordyceps militaris is used as medicine
History is not long, but Cordyceps militaris and cordyceps sinensis are biologically belonging to a category together, have it is very same or analogous it is chemical into
Point, and the component content beneficial to human body such as cordycepin, K, Ca, Se, the vitamin of Cordyceps militaris is higher than wild cordyceps.Greatly
The experiment of pharmacology medicine and clinical practice of amount prove that Cordyceps militaris has same or analogous work(to human body as wild cordyceps sinensis
Energy.But, the price of Cordyceps militaris is but well below rare cordyceps sinensis, and this just brings very big market business opportunity to Cordyceps militaris.
Artificial culture has the fruiting bodies of cordyceps militaris of natural form, is close in active ingredient with traditional cordyceps sinensis, contains worm
The special nutrient compositions such as careless element, cordycepic acid, Cordyceps sinensis polysaccharide, SOD, but also contain protein, amino acid, dimension necessary to human body
Raw element and various trace elements.Also, Cordyceps militaris has obtained the national class medicinal material of Chinese medicine one examination ＆ approval production.As people are to Cordyceps militaris
Accreditation, engenders the figure of Cordyceps militaris, this promotes the market demand of Cordyceps militaris constantly to expand on the dining table of common people.
The breeding method of Cordyceps militaris is most important for the product for obtaining homogeneous, good quality and yield.In cultivating process
In, it is also such for selecting optimal sweep bacterium opportunity and suitable bacterium operation of sweeping.
Cordyceps militaris grow media surface and air contact during secretly training room bacterium germination and grow for aerial hyphae, downwards
Grow for Cordyceps militaris mycelia., can be while Chinese caterpillar fungus culture medium nutrition be absorbed if aerial hyphae does not remove by external force
Have an effect on the formation of Cordyceps militaris former base and change and divide, so as to extend the Cordyceps militaris ripe time and easily grow up to lopsided grass.Therefore,
Need to remove aerial hyphae by sweeping bacterium.
The bacterium mode of sweeping of original Cordyceps militaris mycelia is：1）Use sheet metal（Such as size is that the wide 1cm* of long 10cm* are thick
2mm+15cm handles）Or bamboo chip（Such as size is the wide 1cm* thickness 2mm+15cm handles of long 10cm*）Etc. sweeping bacterium；2）Using metal system
Small scratch sweeps bacterium, and it scratches body provided with many one metal wires in one line and parallel to each other, and the end of each wire is towards same side
It is vertical with the plane where wire to the plane where being bent to form rake hook, and rake hook, as shown in Figure 1（For example wire is straight
Footpath is only 0.8-1.2 millimeters, and wire length is 5cm, and rake hook length is 0.9cm, and scratcher handle length is 15cm）.Metal system is small to scratch
It is to prepare to find the culture medium of microbiological contamination.
Aerial hyphae can be cleared away or scratched out by sheet metal or small scratch of bamboo chip and metal, but passed through production repeatedly and tasted
Examination, it is found that the position that sheet metal or bamboo chip scrape aerial hyphae off is rectilinear form, once culture medium charge level is uneven or has indivedual trainings
Base particle projection is supported, will be scraped, or sweep less than some corners or position.In addition, existing metal long-handled hook body region with
Support zone is vertical and gap is big, therefore some total positions are scratched less than this has had a strong impact on batch production during mycelia is scratched
Final yield.
The content of the invention
What the present invention provided a kind of Cordyceps militaris aerial hyphae sweeps bacterium method, it is characterised in that in Cordyceps militaris spawn in culture
To aerial hyphae is produced, simultaneously annesl is that yellow carries out sweeping bacterium but without the growth phase for producing fruit body primordium for culture in base.
Sweeping bacterium method and can shortening the time of cordycep militaris fruiting body entity primodium formation for the Cordyceps militaris that the present invention is provided, and makes pupa
Cordyceps sinensis former base breaks up neat and consistent, so as to shorten the cultivation cycle of Cordyceps militaris, budding is neat, condition is good, improves Cordyceps militaris
Quality and yield.
Further, it is described sweep bacterium sterilized water be stained with by old mycelia remover tool aerial hyphae is unidirectionally swept out, and make culture base table
Aerial hyphae on face lodges in the surface of culture medium.
Further, the old mycelia remover tool includes hairbrush and/or metal is scratched.
Further, the hairbrush is wooden hair planting brush, and the wooden hair planting brush includes brush holder, brush and is fixed on brush
Bristle（As shown in Figure 2）.
Further, the entire length of the wooden hair planting brush is 15-30cm, and width is 15-20cm, and thickness is 1-2cm；Institute
The length for stating bristle is 3-8cm, and it is 15-20cm to plant hair width, and plant gross thickness is 1-2cm（This thickness refers to that batt is planted in accumulation
Thickness）.
Further, the metal is scratched is scratched for metal stainless steel wire, and the metal stainless steel wire is scratched including scratcher handle, scratches this
Body and located at many steel wires in one line and parallel to each other scratched on body, the end of each steel wire is bent to form in the same direction
The plane where plane and steel wire where long-handled hook, and long-handled hook is same plane（As shown in Figure 3）.
Further, the entire length that the metal stainless steel wire is scratched is 15-25cm, and width is 20-25cm；The steel wire
Between spacing be 0.8-1.2cm, gauge of wire is 0.8-1.2mm, and the length of steel wire is 5-10cm, and the length of long-handled hook is
0.7-1cm.
Further, when Chinese caterpillar fungus culture medium surface finds miscellaneous bacteria mildew, quick lime is noted at mildew, and scratched with metal
The aerial hyphae for opening and not contaminating miscellaneous bacteria is scratched, makes aerial hyphae lodging on Chinese caterpillar fungus culture medium surface.
Further, the area for noting quick lime is 4-6 times of miscellaneous bacteria mildew.
Brief description of the drawings
Fig. 1 is the small structural representation scratched of existing metal.
Fig. 2 is the structural representation of the wooden hair planting brush used in the present invention.
The structural representation that Fig. 3 scratches for the metal stainless steel wire that uses in the present invention.
Embodiment
Cordyceps militaris only stimulates the generation of Cordyceps militaris former base in growth course by temperature, illumination etc., can greatly prolong pupa
Cordyceps sinensis goes out the time of former base, so as to influence fruiting bodies of cordyceps militaris maturation time.The present inventor is by the summing up experience hair that makes repeated attempts
It is existing, the aerial hyphae after the annesl of Cordyceps militaris is carried out to sweep bacterium, the generation of Cordyceps militaris mycelia can be obviously stimulated, typically
It can be seen that Cordyceps militaris former base was grown at 2 days or so, ripe can harvest within 50-55 days, shorten growth cycle, saved cultivation
Cost, and Time To Market is enriched, meet the market demand.
By the present invention in that scratching the feelings that complete Chinese caterpillar fungus culture medium top layer aerial hyphae in annesl with sweeping bacterium brush and sweeping bacterium
Swept out under condition, the growth of Cordyceps militaris mycelia can be stimulated, and then stimulate the growth of Cordyceps militaris former base, greatly shorten Cordyceps militaris length former
The time of base, so as to shorten the fruiting bodies of cordyceps militaris ripe time.
The wooden hair planting brush and metal stainless steel wire that the present invention is used are scratched, and the former wooden hair planting brush plants hair softness, is sweeping
Can be with 360 degree without dead angle except on aerial hyphae, and the size of hair planting brush, one-way operation two can be set according to the size of culture medium
It is secondary to complete；The width that the latter's metal stainless steel wire is scratched also can be with being configured that the width of culture medium matches, steel wire
The long-handled hook for scratching steel wire formation of the front end steel wire with extending is in the same plane, and 90 ° of formation of end bend of preferably steel wire are scratched
Hook, i.e., each long-handled hook is into a line（As shown in Figure 3）.When scratching out aerial hyphae, between scratching between coupler body and the steel wire for supporting it
Gap is small, can more fully scratch more positions.Also, flexible and elasticity due to steel wire length length partially, are running into charge level
Can very flexible fine motion up and down during uneven or culture medium particle projection.They are metal or bamboo in pliability and coverage
What piece can not compare.The aerial hyphae on culture medium after inswept can completely lodge in media surface, culture medium
Grain is intact, without departing from culture medium.Wooden hair planting brush and metal stainless steel wire is scratched in the later stage in use, can be by blending dichloro
The clean mould smart sterilization of sodium isocyanurate, to prevent living contaminants, is greatly lowered contamination rate.It is easy to operate that this sweeps bacterium method,
Human resources can be saved, unnecessary economic loss is reduced.
Sheet metal or bamboo chip, traditional metal scratch cleaning aerial hyphae it is not thorough, cause the Cordyceps militaris maturity period in later stage to be differed
Cause, Cordyceps militaris abnormal rate is 5% or so, and the abnormal rate for sweeping the Cordyceps militaris that bacterium method is cultivated using the present invention is only 0.02% left
The right side, Cordyceps militaris list box net weight improves 2g or so.
In a specific embodiment, the specific size of the wooden hair planting brush is：Entire length is 25cm, width
For 16.5cm, thickness is 1.5cm；Hair planting brush bristle position staple length is 5cm, and it is 16.5cm to plant hair width, and plant gross thickness is 1.5cm
（This thickness refers to that the thickness of batt is planted in accumulation）.Bristle is preferred to use the good binder of viscosity and binded, and with resistance to dissolubility,
Do not loosen, not running, floating brush fall no fracture of wire and running.Carried out to avoid using when Cordyceps militaris sweeps bacterium process with hair planting brush
Running hairbrush, so as not to the later stage cause miscellaneous bacteria to infect.
In a specific embodiment, metal stainless steel wire scratches specific size and is：Entire length is 21cm, width
For 22cm, gauge of wire is 1mm, and the length of steel wire is that the spacing between 7cm, steel wire is 1cm, the formation of steel wire end bend
The length of long-handled hook is 0.7cm.The size that the wooden hair planting brush and metal stainless steel wire that the present invention is used are scratched can be according to culture
The setting change of base size, other adapt to size range in the range of inventive article use, not illustrated separately herein.
The embodiment to the present invention is described in detail below, it should be appreciated that described herein to implement
Mode is merely to illustrate and explain the present invention, and is not intended to limit the invention.
In the present invention, as well known in the art, in order to avoid living contaminants, the various operations carried out are preferably in base
Carried out under conditions of this is sterile, wooden hair planting brush and metal stainless steel wire used is scratched first to be sterilized with sodium dichloro cyanurate
Powder（Active constituent content is 2.5% ± 0.25%）1:10（That is 1 kg of water:10g disinfectant powders）Ratio carry out sterilizing 4 it is small
When, taking-up instrument and material be placed in it is sterile between dry, carry out disinfection processing, make for p=2.144g/l disinfection room in ozone concentration
The clean mould essence of sodium dichloro cyanurate is used before being scratched with wooden hair planting brush and metal stainless steel wire again（Active constituent content：
40%）According to 1:3（That is 1 kg of water:The clean mould essences of 3g）Soaking disinfection 4 hours.
Wherein, the Cordyceps militaris spawn can be commercially available various Cordyceps militaris spawns, and the method for invention of the invention is to pupa
The selection of cordyceps species is not specially required.
Wherein, the Cordyceps militaris spawn can be before culture medium be inoculated in, according to the conventional method of this area, training in advance
Support the form of the form, preferably liquid spawn for solid spawn or liquid spawn.Wherein, the Cordyceps militaris spawn can be according to
Conventional method is inoculated in culture medium, and the present invention is not required particularly.
Culture medium needs to be put into environment culture 10-12 days of shading after strain has been connect, and temperature is at 17-19 DEG C, and humidity is protected
Hold 60%, when Chinese caterpillar fungus culture medium charge level covers with snow-white fish scale-shaped aerial hyphae, and charge level bottom Cordyceps militaris mycelia is
Through taking root completion, it is possible to Chinese caterpillar fungus culture medium is transferred into the culture room equipped with incandescent lamp and carries out seeing that light annesl is handled.
What Cordyceps militaris took root downwards extension is exactly Cordyceps militaris mycelia, and grow culture medium is exactly aerial hyphae, when seeing
Magazine cover with the uniform fish scale-shaped aerial hyphae of snowy white and upset magazine find magazine bottom into rule it is netted when, that is, send out
Bacterium completes.
Mycelia and aerial hyphae continued growth simultaneously in culture medium, until the state that the mycelia of Cordyceps militaris takes root is reached,
It is preferred that carrying out the culture in light culture stage.Now light culture is light protected environment.The culture in the light culture stage can be from routine
Selected in the method for Tissue Culture of Cordyceps militaris, as long as being carried out under no light condition.The light culture stage need to meet following condition：
Temperature is 15-20 DEG C, preferably 17-19 DEG C；Relative humidity is 50%-70%, preferably 60%.Conventional light culture cultivation stage
For 10-12 days.
In accordance with the present production process, it could carry out artificial sweeping bacteria thorn in the case where aerial hyphae is changed into yellow
Swash.In order to shorten the time that aerial hyphae annesl is yellow, following steps can be carried out：Light stimulation is carried out, it is white by room is cultivated
Vehement lamp is opened, and daily light application time is 16-19 hours, and intensity of illumination is 100-150 Luxs, and the temperature of illumination period is
18-25 DEG C, be optimal with 20 DEG C；Temperature during no light is 18-25 DEG C, is optimal with 22 DEG C, relative air humidity is 50-
70%, optimum humidity is 60%.The conventional light stimulation time is 36-48 hours.Visible Chinese caterpillar fungus culture medium gas after 36 hours
Raw mycelia is gradually converted into yellow.
The growth of Cordyceps militaris mycelia can be stimulated by manually sweeping bacterium, the formation of Cordyceps militaris former base is stimulated, and shorten pupa worm
The time of the long former base of grass, but it is artificial sweep bacteria thorn and swash can not carry out at any time.First, manually sweep bacterium when
The aerial hyphae that must assure that on Chinese caterpillar fungus culture medium is waited to have succeeded annesl yellowly, once do not complete can not for this step
Bacterium is swept in progress.In addition, Cordyceps militaris aerial hyphae also may proceed to growth between culture, Cordyceps militaris aerial hyphae is found during annesl
It is higher by media surface 0.02mm or so, and the single fish scale-shaped area rided out is when being 0.6-0.9 square millimeter, now illumination turn
Color range section terminates, and can enter the artificial stage for sweeping the sharp Cordyceps militaris mycelial growth of bacteria thorn.
Bacterium instrument is swept used in the inventive method：Wooden hair planting brush（The long wide 18cm* thickness 1cm of 25cm*）With metal system
After stainless steel wire is scratched the step of by thorough disinfection, bacterium operation is swept in unidirectional progress, can not carry out flyback repeatedly in this process
Brush or scratch, should try one's best makes the aerial hyphae of Cordyceps militaris lodge direction unanimously, bacterium depth is swept for 2.2-2.6mm, according to the thin of bacterium germination
Thickness, can not exert oneself very much, to prevent from scraping off culture medium.
The method according to the invention, wherein, the instrument that the surface of the aerial hyphae is swept out can be wooden hair planting brush
At least one of scratched with metal stainless steel wire.The situation of miscellaneous bacteria is infected according to culture medium, for the available wood that charge level is intact
Hair planting brush processed is stained with the aerial hyphae that sterilized water sweeps out Cordyceps militaris surface, it is found that culture medium charge level has miscellaneous bacteria that mildew then is noted into raw
Lime（Quick lime cold water is with uniformly, and note quick lime area need to be 5 times of miscellaneous equal mildew, it is ensured that miscellaneous bacteria mildew is completely covered,
Prevent the later stage from propagating infection）, then scratched with stainless steel wire and scratch out aerial hyphae, make aerial hyphae lodging in Chinese caterpillar fungus culture medium
On surface.
Below by by implementing the detailed description to present invention progress example.
Embodiment
Culture medium is obtained after wheat 350g, water 560ml are mixed, putting culture medium under 121 DEG C and 0.14Mpa steam goes out
Room temperature is cooled to after bacterium 90Min, culture medium is obtained standby.
The process that culture medium inoculated is completed on superpurgative working table streamline, then carries out the culture in light culture stage.
The light culture stage need to meet following condition：Temperature is 18 DEG C；Relative humidity is 60%.Chinese caterpillar fungus culture medium charge level covers with snow-white
Fish scale-shaped aerial hyphae, and charge level bottom Cordyceps militaris mycelia has taken root completion, and just Chinese caterpillar fungus culture medium is transferred to equipped with white
The culture room of vehement lamp carries out seeing that light annesl is handled.Light culture cultivation stage was completed in 10-12 days.
Next culture room carry out illumination annesl culture, in order to faster make aerial hyphae annesl for yellow, it is necessary to enter
Portable lighter light irradiation culture：Daily light application time is 17 hours, and intensity of illumination is 100 Luxs, and the temperature of illumination period is 20
℃；Temperature during no light is 20 DEG C, and relative air humidity is 60%.Find that Cordyceps militaris aerial hyphae is higher by during annesl
Media surface 0.02mm or so, and the fish scale-shaped area rided out be 0.6-0.9 square millimeter when, now the illumination annesl stage knot
Beam, can enter artificial bacteria thorn of sweeping and swash Cordyceps militaris vegetative stage.
Bacterium instrument is swept used in the inventive method：Wooden hair planting brush（The long wide 18cm* thickness 1cm of 25cm*）With metal system
Stainless steel wire carries out sweeping bacterium after scratching the step of by thorough disinfection.The process for sweeping bacterium is one-way operation, it is impossible to carry out flyback repeatedly
Brush or scratch, the aerial hyphae of Cordyceps militaris is lodged direction unanimously, sweep bacterium depth for 2.4mm.It can not exert oneself very much, to prevent from cultivating
Base is scraped off.
Cordyceps militaris is easily infected in growth course by miscellaneous bacteria, and wooden hair planting brush and metal stainless steel wire used is scratched
First use Sodium DichloroisocyanurateDisinfection Disinfection Powder（Active constituent content is 2.5% ± 0.25%）1:10（That is 1 kg of water:10g disinfectant powders）
Ratio carry out sterilizing 4 hours, taking-up instrument and material be placed in it is sterile between dry, be p=2.144g/l's in ozone concentration
Disinfection room carries out disinfection processing, clean mould with sodium dichloro cyanurate again before being scratched using wooden hair planting brush and metal stainless steel wire
Essence（Active constituent content：40%）According to 1:3（That is 1 kg of water:The clean mould essences of 3g）Soaking disinfection 4 hours.
To change wooden hair planting brush in time when sweeping to the culture medium charge level for being infected with miscellaneous bacteria with wooden hair planting brush, and by microbiological contamination
Wooden hair planting brush emphasis carry out disinfection processing, preparing for disinfectant must be 2 times of usually concentration.
In order to ensure not cross-infection and reduction later stage contamination rate, wooden hair planting brush and metal stainless steel wire are being used
During scratching, often sweep sharp 50 boxes of bacteria thorn and scratch and sweep bacterium water it is necessary to change wooden hair planting brush and metal stainless steel wire.
Sweep and the processing that carries out disinfection is scratched to used wooden hair planting brush and metal stainless steel wire after bacterium stimulation period terminates, with
Just next time uses, and disinfectant program is：First use Sodium DichloroisocyanurateDisinfection Disinfection Powder（Active constituent content is 2.5% ± 0.25%）1:10
（That is 1 kg of water:10g disinfectant powders）Ratio carry out sterilizing 4 hours, taking-up instrument and material be placed in it is sterile between dry,
Ozone concentration carries out disinfection processing for p=2.144g/l disinfection room.It is scratched using wooden hair planting brush and metal stainless steel wire
Before, again with the clean mould essence of sodium dichloro cyanurate（Active constituent content：40%）According to 1:3（That is 1 kg of water:The clean mould essences of 3g）Leaching
Bubble sterilization 4 hours.
Now manually sweep the sharp Cordyceps militaris vegetative stage of bacteria thorn to terminate, being next just into induction stimulates pupa worm
The grass growth former base stage.
Induction Cordyceps militaris grows the condition in former base stage：Daily light application time is 17 hours, and intensity of illumination is 100 Le gram
Department, illumination period temperature is 22 DEG C.Temperature during no light is 18 DEG C, and relative air humidity is 70%.Former base induction period 3-
4 days, it is seen that fruit body primordium is initially formed on aerial hyphae（The projection of rice-shaped）, now, terminate the cultivation of former base induction period
Training culture, then carries out the cultivation culture in sporophore growth stage.
Requirement of the fructification in growth phase to temperature and humidity is higher, and temperature and humidity now concerns pupa
Length, weight of cordyceps sinensis later stage fructification etc., so now the condition in sporophore growth stage is including daily light application time
17 hours, intensity of illumination was 150 Luxs, and the temperature of illumination period is 22 DEG C, and the temperature during no light is 20 DEG C, relatively empty
Air humidity degree is 85%.From the 50-55 days after inoculation, fructification top conidia powder was all blown successively, as overgrowing with thorn one
Sample, Cordyceps militaris is ripe, and nutritive value is of a relatively high, can terminate the culture in fruiting bodies of cordyceps militaris stage, be harvested.
In the present embodiment, from initial charging production to final harvesting, by 50-55 days, each culture medium was averagely received
35g dry fruiting bodies of cordyceps militaris is obtained, single culture medium fructification weight differential is in 1-2g.
Comparative example
According to the step of embodiment 1 and condition carry out Cordyceps militaris culture, except that the light stimulation stage terminate after do not have
Artificial bacteria thorn of sweeping is carried out to culture medium to swash, and is not swept out the surface of aerial hyphae, but be directly entered culture medium former base
Culture, daily light application time is 17 hours, and intensity of illumination is 100 Luxs, and illumination period temperature is 22 DEG C.During no light
Temperature be 18 DEG C, relative air humidity is 70%.Former base culture 10-12 days, is just shown on aerial hyphae and initially forms fructification original
Base（The projection of rice-shaped）.Now, terminate the cultivation culture of former base induction period, then carry out the cultivation in sporophore growth stage
Culture.It is observed that the quantity that former base is gone out is few, uneven.
Next fructification cultivation stage is entered, the wherein condition in sporophore growth stage is including daily light application time
17 hours, intensity of illumination was 150 Luxs, and the temperature of illumination period is 22 DEG C, and the temperature during no light is 20 DEG C, relatively empty
Air humidity degree is 85%.After the 20-30 days stage of sporophore growth, it is seen that the size of the fructification of Cordyceps militaris is no longer grown up substantially, still
Fructification shape is strange, and growth is uneven, and spinule occurs in top, terminates the culture in fructification stage, is harvested.According to
Observation, the media surface fructification rareness not swashed through overscan bacteria thorn, sporophore shape variation is sprouted irregular.
In this comparative example, from initial charging production to final harvesting, by 55-60 days, each culture medium was averagely received
23g dry fruiting bodies of cordyceps militaris is obtained, single culture medium fructification weight differential is in 5-6g.
Conclusion：By the comparison of embodiment and comparative example, under same illumination, temperature, humidity condition, by this
There is morning and evening, sporophore growth form, son in fact in growth cycle, fructification in the Cordyceps militaris that inventive method cleaning aerial hyphae is cultivated
The single weight of body collecting time, fructification（It is dry, single box）With the Cordyceps militaris for being superior to cultivate without overscan bacterium in weight differential.Tool
Body：
1）By manually sweeping bacterium, it is stained with sterilized water with wooden hair planting brush and metal stainless steel wire scratches the culture medium 3-4 of stimulation
Its visible formation fruit body primordium（The projection of rice-shaped）.Without being seen by manually sweeping the sharp culture medium 10-12 talent of bacteria thorn
Fruit body primordium is initially formed on aerial hyphae（The projection of rice-shaped）.
2）Shortened by the culture basal growth fructification time of artificial stimulation, the fructification shape grown is uniformly neat, and
Aerial hyphae is not swept out by manually sweeping the sharp culture medium of bacteria thorn, the fructification shape of growth is strange, and grows week
Phase is long.It can be harvested by the 50-55 days by manually sweeping the sharp fruiting bodies of cordyceps militaris of bacteria thorn, without by manually sweeping
The sharp fruiting bodies of cordyceps militaris for sweeping out aerial hyphae of bacteria thorn will be by that could be harvested for 55-60 days.
3）Fruiting bodies of cordyceps militaris list box dry product average out to 35g/ boxes in embodiment, difference is 1-2g, pupa worm between box and box
Careless abnormal rate is only 0.02%, fruiting bodies of cordyceps militaris list box dry product average out to 23g/ boxes in comparative example, and difference is 5- between box and box
6g, Cordyceps militaris abnormal rate is 19%.
Therefore, the operation that the present invention sweeps out aerial hyphae, for improving fruiting bodies of cordyceps militaris yield, shortens Cordyceps militaris
Entity growth cycle, promotes fruiting bodies of cordyceps militaris form perfection to serve very crucial effect.The present invention can shorten pupa worm
The division time of grassland base, divide cordycep militaris fruiting body entity primodium consistent, reduce the difference between box and box, improve single box production
Amount.
It is further to note that carrying out manually sweeping bacterium, during sweeping out Chinese caterpillar fungus culture medium aerial hyphae, it is ensured that
It is thorough that all instruments are sterilized, for example：The various operations carried out are preferably carried out under conditions of substantially sterile, and used is each
The instrument of kind and material first use Sodium DichloroisocyanurateDisinfection Disinfection Powder（Active constituent content is 2.5% ± 0.25%）1:10（I.e. 1 kilogram
Water:10g disinfectant powders）Ratio carry out sterilizing 4 hours, take out wooden hair planting brush and metal stainless steel wire scratch be placed in it is sterile
Between dry, carried out disinfection processing for p=2.144g/l disinfection room in ozone concentration, using before instrument and material again with two
The clean mould essence of symclosene sodium（Active constituent content：40%）According to 1:3（That is 1 kg of water:The clean mould essences of 3g）Soaking disinfection 4 hours.
The personnel of operation will also be carried out in strict accordance with operating process, entered and exited culture room and wanted timely opponent to clean, and and
When replace with clothes, shoes, gloves, the mouth mask of sanitized（It can be carried out disinfection in advance with ultraviolet lamp or ozone machine）.Avoid
Cross-infection and miscellaneous bacteria infection.
Carrying out artificial sweep during bacterium sweeps out Chinese caterpillar fungus culture medium aerial hyphae, it is ensured that all to sweep the sterilization of bacterium instrument thorough
While bottom, used tool is more for several sets, such as finds that culture medium charge level is abnormal, and bacterium instrument of sweeping will be changed in time, and by
The bacterium instrument of sweeping acted on abnormal culture medium is individually repeatedly disinfected, and it is conventional configuration to be needed in the proportioning of disinfectant
2 times of concentration, and the bacterium instrument of sweeping of sanitized carries out Detection, checks whether disinfection thoroughly, for abnormal tight
Weight is swept bacterium instrument and abandoned in time, and what is more renewed sweeps bacterium instrument.
Process and contrast of the present invention described in detail above in embodiment, detail of the present invention are not limited to
This, operating process can be deformed according to actual conditions, and these simple deformations belong to protection scope of the present invention.In not lance
It can be combined in the case of shield.
Generality data in this invention are drawn by experimental summary repeatedly, with ensure the degree of accuracy of the data obtained with
And general geological coodinate system.
Claims (9)
1. a kind of Cordyceps militaris aerial hyphae sweeps bacterium method, it is characterised in that cultivated in the medium to production in Cordyceps militaris spawn
Bacterium is swept in the growth phase progress of raw aerial hyphae and annesl for yellow but without generation fruit body primordium.
2. Cordyceps militaris aerial hyphae according to claim 1 sweeps bacterium method, it is characterised in that described to sweep bacterium by sweeping bacterium
Apparatus is stained with sterilized water and unidirectionally sweeps out aerial hyphae, and the aerial hyphae in media surface is lodged in the table of culture medium
Face.
3. Cordyceps militaris aerial hyphae according to claim 2 sweeps bacterium method, it is characterised in that the old mycelia remover tool includes
Hairbrush and/or metal are scratched.
4. Cordyceps militaris aerial hyphae according to claim 3 sweeps bacterium method, it is characterised in that the hairbrush is planted to be wooden
Hairbrush, the wooden hair planting brush includes brush holder, brush and the bristle being fixed on brush.
5. Cordyceps militaris aerial hyphae according to claim 4 sweeps bacterium method, it is characterised in that the wooden hair planting brush
Entire length is 15-30cm, and width is 15-20cm, and thickness is 1-2cm；The length of the bristle is 3-8cm, plants hair width and is
15-20cm, plant gross thickness is 1-2cm.
6. Cordyceps militaris aerial hyphae according to claim 3 sweeps bacterium method, it is characterised in that the metal stainless steel
Silk is scratched including scratcher handle, scratches body and located at many steel wires in one line and parallel to each other scratched on body, the end of each steel wire
The plane being bent to form in the same direction where long-handled hook, and long-handled hook and the plane where steel wire are same plane.
7. Cordyceps militaris aerial hyphae according to claim 6 sweeps bacterium method, it is characterised in that the metal stainless steel
The entire length that silk is scratched is 15-25cm, and width is 20-25cm；Spacing between the steel wire is 0.8-1.2cm, steel wire it is straight
Footpath is 0.8-1.2mm, and the length of steel wire is 5-10cm, and the length of long-handled hook is 0.7-1cm.
8. Cordyceps militaris aerial hyphae according to any one of claim 1 to 7 sweeps bacterium method, it is characterised in that when pupa worm
When careless media surface finds miscellaneous bacteria mildew, in quick lime on note at mildew, and scratched with metal and open the gas for not contaminating miscellaneous bacteria life
Mycelia, makes aerial hyphae lodging on Chinese caterpillar fungus culture medium surface.
9. Cordyceps militaris aerial hyphae according to claim 8 sweeps bacterium method, it is characterised in that the area of note quick lime is
4-6 times of miscellaneous bacteria mildew.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283477A (en) * | 2012-02-24 | 2013-09-11 | 北京市弘科农场 | Method for producing cordyceps militaris |
| CN104170650A (en) * | 2014-08-14 | 2014-12-03 | 安徽亿源中药饮片科技有限公司 | Cordyceps militaris industrialized artificial cultivation method |
| CN204482433U (en) * | 2015-02-23 | 2015-07-22 | 江苏康能生物工程股份有限公司 | The electronic mycelium stimulation brush of Cordyceps militaris |
| CN105309199A (en) * | 2014-07-30 | 2016-02-10 | 庄芳 | Black fungus cultivation method |
| CN106171523A (en) * | 2016-07-26 | 2016-12-07 | 河北北尚农业科技有限公司 | The breeding method of Cordyceps militaris |
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2017
- 2017-05-20 CN CN201710360079.2A patent/CN107094497A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283477A (en) * | 2012-02-24 | 2013-09-11 | 北京市弘科农场 | Method for producing cordyceps militaris |
| CN105309199A (en) * | 2014-07-30 | 2016-02-10 | 庄芳 | Black fungus cultivation method |
| CN104170650A (en) * | 2014-08-14 | 2014-12-03 | 安徽亿源中药饮片科技有限公司 | Cordyceps militaris industrialized artificial cultivation method |
| CN204482433U (en) * | 2015-02-23 | 2015-07-22 | 江苏康能生物工程股份有限公司 | The electronic mycelium stimulation brush of Cordyceps militaris |
| CN106171523A (en) * | 2016-07-26 | 2016-12-07 | 河北北尚农业科技有限公司 | The breeding method of Cordyceps militaris |
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