CN107087455A - A kind of assay method of levisticum seed vigor - Google Patents
A kind of assay method of levisticum seed vigor Download PDFInfo
- Publication number
- CN107087455A CN107087455A CN201710354712.7A CN201710354712A CN107087455A CN 107087455 A CN107087455 A CN 107087455A CN 201710354712 A CN201710354712 A CN 201710354712A CN 107087455 A CN107087455 A CN 107087455A
- Authority
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- China
- Prior art keywords
- seed
- levisticum
- embryo
- dyeing
- degrees celsius
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000212321 Levisticum Species 0.000 title claims abstract description 37
- 238000003556 assay Methods 0.000 title claims abstract description 8
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000004043 dyeing Methods 0.000 claims abstract description 12
- 230000035899 viability Effects 0.000 claims abstract description 12
- 238000005520 cutting process Methods 0.000 claims abstract description 7
- 238000004040 coloring Methods 0.000 claims abstract description 5
- 239000008363 phosphate buffer Substances 0.000 claims abstract description 5
- 230000017074 necrotic cell death Effects 0.000 claims description 4
- 208000036119 Frailty Diseases 0.000 claims description 2
- 206010003549 asthenia Diseases 0.000 claims description 2
- 239000000975 dye Substances 0.000 claims description 2
- 230000013020 embryo development Effects 0.000 claims description 2
- 238000002203 pretreatment Methods 0.000 claims description 2
- 238000001514 detection method Methods 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract description 2
- 230000035784 germination Effects 0.000 description 9
- 238000005286 illumination Methods 0.000 description 6
- 230000007226 seed germination Effects 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 241001254604 Angelica pubescens Species 0.000 description 1
- 241000125183 Crithmum maritimum Species 0.000 description 1
- 238000012550 audit Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- VIKNJXKGJWUCNN-XGXHKTLJSA-N norethisterone Chemical compound O=C1CC[C@@H]2[C@H]3CC[C@](C)([C@](CC4)(O)C#C)[C@@H]4[C@@H]3CCC2=C1 VIKNJXKGJWUCNN-XGXHKTLJSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
Abstract
The invention discloses a kind of assay method of levisticum seed vigor, comprise the following steps：Levisticum seed is immersed in clear water 8 12 hours, filtered out standby；Rip cutting is carried out along seed dorsal midline, embryo and endosperm are divided into two parts, a portion is chosen, it is put into what is configured by phosphate buffer, concentration is in 0.6% 1% TTC solution, in being dyed under 25 degrees Celsius of 45 degrees Celsius of constant temperatures, dyeing time is 50 minutes；After dyeing terminates, seed 2 or 3 times are rinsed with running water, embryo coloring case is observed immediately, judges seed whether there is viability.The present invention provides a fast and convenient method for the detection of levisticum seed quality, is easy in levisticum seed circulation and the seed quality Rapid identification in production.
Description
Technical field
The present invention relates to field of biological detection, and in particular to a kind of assay method of levisticum seed vigor.
Background technology
Levisticum is the dry root of samphire Angelica pubescens, is China's parts of generic medicinal plants, artificial growth existing more than 300
Year history；Main product is saved in Hubei, Gansu etc., and cultivated area is up to more than 100,000 mu, it has also become the pillar industry in local rural area.Market
The levisticum seed of upper circulation is generally the scattered production of peasant household, and levels of audit quality is uneven, is that we once use germination percentage, mass of 1000 kernel, net this
Degree, four indexs of water content, have formulated the quality classification standard of levisticum seed, there is presently no the measure of levisticum seed vigor
The assay method of technology, only levisticum seed germination rate.But levisticum Seed Germination Test needs one-month period, the cycle is longer, together
When levisticum seed not storage tolerance, seed quality reduces with the elongated of storage time.
The content of the invention
To solve the above problems, the invention provides a kind of assay method of levisticum seed vigor.
To achieve the above object, the technical scheme taken of the present invention is：
A kind of assay method of levisticum seed vigor, comprises the following steps：
S1, dyeing pre-treatment：Levisticum seed is immersed in clear water 8-12 hours, filtered out standby；
S2, dyeing：Rip cutting is carried out along seed dorsal midline, embryo and endosperm are divided into two parts, a portion is chosen,
Be put into what is configured by phosphate buffer, concentration for 0.6%-1% TTC solution in, in 25 degrees Celsius of -45 degrees Celsius of constant temperatures
Lower dyeing, dyeing time is 50 minutes；
After S3, dyeing terminate, seed 2 or 3 times are rinsed with running water, embryo coloring case is observed immediately, judges that seed has
Without viability, criterion is：
Embryonic development is good, complete, whole embryo dyes cerise；Cotyledon have fraction necrosis, its position be not embryo axis and
Cotyledon junction；Though embryo has fraction necrosis, other positions are intact, to there is viability seed；Other be debility or
Blind seed.
The invention has the advantages that：
For levisticum seed quality detection provide a fast and convenient method, be easy in levisticum seed circulation with production
Seed quality Rapid identification.
Embodiment
In order that objects and advantages of the present invention are more clearly understood, the present invention is carried out with reference to embodiments further
Describe in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this hair
It is bright.
In following examples, the germination rate formula for speculating levisticum seed according to levisticum seed vigor is：Germination rate=
((viable seed percentage * 0.73-0.0016) ± 0.04) * 100%.
Embodiment 1
On 2 5th, 2017 21 points, fetch the levisticum seed 4g from the east of Sichuan water cloth a strip of land between hills town Yang Jiaya villages, be put into fill 100 milli
Rise in the beaker of running water and soak.
On 2 6th, 2017 8 points, the levisticum seed of immersion is filtered out, is placed on blotting paper, levisticum seed 150 is randomly selected
Grain, is divided into 3 groups, every group 50；Levisticum seed is subjected to rip cutting along seed dorsal midline with scalpel, embryo and endosperm are divided into
Two halves, choose wherein half, abandon second half；Packet is put into what is configured by phosphate buffer after cutting, and concentration is 0.6%, temperature
Spend in the TTC solution for 35 degrees Celsius, in dyeing under 35 degrees Celsius of constant temperatures.
After 50 minutes, levisticum seed is filtered out, seed is rinsed 3 times with running water, embryo coloring case is observed immediately, judges to plant
Son whether there is viability.
Three groups of viable seed amounts are respectively 24,26,30, and average viability is 53.3%.
After viability test terminates, remaining seed is sterilized 2.5 minutes in 75% alcohol, laggard running water rinses 3
It is secondary, then randomly select in 150, the culture dish that the moistening filter paper that haves three layers is put as three, each 50, culture dish；By culture dish
It is placed in 25 degrees Celsius of constant temperature illumination boxs, daily illumination 8 hours, intensity of illumination 2500Lux；Culture dish is checked daily, is protected
Filter paper moistening is held, and counts seed and sprouts quantity.
On March 7th, 2017, sprouting test terminates, the levisticum seed germination rates of three culture dishes is respectively 32%, 38%,
36%, average germination rate is 35.3%.
The viability of this batch of levisticum seed is 53.3%, thus it is speculated that germination rate is：((53.3%*0.73-0.0016) ±
0.04) * 100%=38.75% ± 4%；It is 35.3% to survey germination rate.
Embodiment 2
On 2 9th, 2017 22 points, fetch the levisticum seed 4g from Huating Gansu county Ma Xia towns Jiang Zhuan Villages, be put into and fill
Soaked in the beaker of 80 milliliters of running water.
On 2 10th, 2017 8 points, the levisticum seed of immersion is filtered out, is placed on blotting paper, levisticum seed is randomly selected
150, it is divided into 3 groups, every group 50；Levisticum seed is subjected to rip cutting along seed dorsal midline with scalpel, embryo and endosperm is equal
It is split into two halves, chooses wherein half, abandon second half；Packet is put into what is configured by phosphate buffer after cutting, and concentration is
0.8%th, temperature is in 45 degrees Celsius of TTC solution, in being dyed under 45 degrees Celsius of constant temperatures.
After 50 minutes, levisticum seed is filtered out, seed is rinsed 3 times with running water, embryo coloring case is observed immediately, judges to plant
Son whether there is viability.
Three groups of viable seed amounts are respectively 20,22,19, and average viability is 40.7%.
After viability test terminates, remaining seed is sterilized 2.5 minutes in 75% alcohol, laggard running water rinses 4
It is secondary, then randomly select in 150, the culture dish that the moistening filter paper that haves three layers is put as three, each 50, culture dish；By culture dish
It is placed in 25 degrees Celsius of constant temperature illumination boxs, daily illumination 8 hours, intensity of illumination 2500Lux；Culture dish is checked daily, is protected
Filter paper moistening is held, and counts seed and sprouts quantity.
On April 6th, 2017, sprouting test terminates, the levisticum seed germination rates of three culture dishes is respectively 26%, 30%,
30%, average germination rate is 28.7%.
The viability of this batch of levisticum seed is 40.7%, thus it is speculated that germination rate is：((40.7%*0.73-0.0016) ±
0.04) * 100%=29.6% ± 4%；It is 28.7% to survey germination rate.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (1)
1. a kind of assay method of levisticum seed vigor, it is characterised in that comprise the following steps：
S1, dyeing pre-treatment：Levisticum seed is immersed in clear water 8-12 hours, filtered out standby；
S2, dyeing：Rip cutting is carried out along seed dorsal midline, embryo and endosperm are divided into two parts, a portion is chosen, is put into
Configured by phosphate buffer, concentration for 0.6%-1% TTC solution in, under 25 degrees Celsius of -45 degrees Celsius of constant temperatures contaminate
Color, dyeing time is 50 minutes；
After S3, dyeing terminate, seed 2 or 3 times are rinsed with running water, embryo coloring case is observed immediately, judges seed whether there is life
Vigor, criterion is：
Embryonic development is good, complete, whole embryo dyes cerise, and cotyledon has fraction necrosis, and its position is not embryo axis and cotyledon
Junction, though embryo has fraction necrosis, other positions are intact, to there is viability seed；Other are debility or dead kind
Son.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710354712.7A CN107087455A (en) | 2017-05-11 | 2017-05-11 | A kind of assay method of levisticum seed vigor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710354712.7A CN107087455A (en) | 2017-05-11 | 2017-05-11 | A kind of assay method of levisticum seed vigor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107087455A true CN107087455A (en) | 2017-08-25 |
Family
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|---|---|---|---|
| CN201710354712.7A Pending CN107087455A (en) | 2017-05-11 | 2017-05-11 | A kind of assay method of levisticum seed vigor |
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| CN (1) | CN107087455A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109115955A (en) * | 2018-08-24 | 2019-01-01 | 徐长斌 | A kind of wheat seed quality determining method |
| CN109632433A (en) * | 2018-11-19 | 2019-04-16 | 安徽丰絮农业科技股份有限公司 | A method of for detecting cotton seeds quality |
| CN112930760A (en) * | 2021-04-05 | 2021-06-11 | 兰州大学 | Tetrazole determination method for sweet pepper seed viability |
| CN117813965A (en) * | 2024-03-05 | 2024-04-05 | 蒙草生态环境(集团)股份有限公司 | Method for promoting germination of seeds of radix angelicae pubescentis |
| CN117813965B (en) * | 2024-03-05 | 2024-05-17 | 蒙草生态环境(集团)股份有限公司 | Method for promoting germination of seeds of radix angelicae pubescentis |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103703890A (en) * | 2013-12-19 | 2014-04-09 | 甘肃凯源生物技术开发中心 | Determination method for viability of daphne giraldii seeds |
-
2017
- 2017-05-11 CN CN201710354712.7A patent/CN107087455A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103703890A (en) * | 2013-12-19 | 2014-04-09 | 甘肃凯源生物技术开发中心 | Determination method for viability of daphne giraldii seeds |
Non-Patent Citations (1)
| Title |
|---|
| 沈宇峰 等: "白术种子生活力测定方法及其与发芽率的相关性研究", 《中国中药杂志》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109115955A (en) * | 2018-08-24 | 2019-01-01 | 徐长斌 | A kind of wheat seed quality determining method |
| CN109632433A (en) * | 2018-11-19 | 2019-04-16 | 安徽丰絮农业科技股份有限公司 | A method of for detecting cotton seeds quality |
| CN112930760A (en) * | 2021-04-05 | 2021-06-11 | 兰州大学 | Tetrazole determination method for sweet pepper seed viability |
| CN112930760B (en) * | 2021-04-05 | 2022-05-03 | 兰州大学 | Tetrazole determination method for sweet pepper seed viability |
| CN117813965A (en) * | 2024-03-05 | 2024-04-05 | 蒙草生态环境(集团)股份有限公司 | Method for promoting germination of seeds of radix angelicae pubescentis |
| CN117813965B (en) * | 2024-03-05 | 2024-05-17 | 蒙草生态环境(集团)股份有限公司 | Method for promoting germination of seeds of radix angelicae pubescentis |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170825 |
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