CN107083393B - 用于在纤毛虫宿主细胞中异源表达病毒蛋白的系统 - Google Patents

用于在纤毛虫宿主细胞中异源表达病毒蛋白的系统 Download PDF

Info

Publication number
CN107083393B
CN107083393B CN201710152797.0A CN201710152797A CN107083393B CN 107083393 B CN107083393 B CN 107083393B CN 201710152797 A CN201710152797 A CN 201710152797A CN 107083393 B CN107083393 B CN 107083393B
Authority
CN
China
Prior art keywords
leu
ser
gly
asn
thr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201710152797.0A
Other languages
English (en)
Chinese (zh)
Other versions
CN107083393A (zh
Inventor
M·哈特曼
C·萨克塞
J·阿佩尔特
U·博考
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cilian AG
Original Assignee
Cilian AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cilian AG filed Critical Cilian AG
Publication of CN107083393A publication Critical patent/CN107083393A/zh
Application granted granted Critical
Publication of CN107083393B publication Critical patent/CN107083393B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/162—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/145—Orthomyxoviridae, e.g. influenza virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C07K14/11—Orthomyxoviridae, e.g. influenza virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16211—Influenzavirus B, i.e. influenza B virus
    • C12N2760/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16211—Influenzavirus B, i.e. influenza B virus
    • C12N2760/16234—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/60—Vectors containing traps for, e.g. exons, promoters

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Epidemiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Pulmonology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Communicable Diseases (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201710152797.0A 2009-06-17 2010-06-15 用于在纤毛虫宿主细胞中异源表达病毒蛋白的系统 Active CN107083393B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0910357.3 2009-06-17
GB0910357A GB2471093A (en) 2009-06-17 2009-06-17 Viral protein expression in ciliates
CN2010800367364A CN102712909A (zh) 2009-06-17 2010-06-15 用于在纤毛虫宿主细胞中异源表达病毒蛋白的系统

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN2010800367364A Division CN102712909A (zh) 2009-06-17 2010-06-15 用于在纤毛虫宿主细胞中异源表达病毒蛋白的系统

Publications (2)

Publication Number Publication Date
CN107083393A CN107083393A (zh) 2017-08-22
CN107083393B true CN107083393B (zh) 2021-12-10

Family

ID=40940892

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201710152797.0A Active CN107083393B (zh) 2009-06-17 2010-06-15 用于在纤毛虫宿主细胞中异源表达病毒蛋白的系统
CN2010800367364A Pending CN102712909A (zh) 2009-06-17 2010-06-15 用于在纤毛虫宿主细胞中异源表达病毒蛋白的系统

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN2010800367364A Pending CN102712909A (zh) 2009-06-17 2010-06-15 用于在纤毛虫宿主细胞中异源表达病毒蛋白的系统

Country Status (11)

Country Link
US (1) US8377682B2 (https=)
EP (1) EP2443232B1 (https=)
JP (1) JP2012529899A (https=)
CN (2) CN107083393B (https=)
CA (1) CA2765799C (https=)
DK (1) DK2443232T3 (https=)
ES (1) ES2627402T3 (https=)
GB (1) GB2471093A (https=)
IN (1) IN2012DN00448A (https=)
PL (1) PL2443232T3 (https=)
WO (1) WO2010146043A2 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2471093A (en) 2009-06-17 2010-12-22 Cilian Ag Viral protein expression in ciliates
WO2011100369A2 (en) * 2010-02-09 2011-08-18 The Trustees Of Columbia University In The City Of New York Methods for altering polypeptide expression and solubility
JP6841184B2 (ja) * 2017-08-07 2021-03-10 日立金属株式会社 半導体装置の製造方法
CN108130340B (zh) * 2018-02-06 2021-02-23 福建省农业科学院畜牧兽医研究所 表达鸭源禽流感病毒ns1蛋白的方法以及该方法的应用
CN114569710A (zh) * 2022-03-09 2022-06-03 华南农业大学 一种基于嗜热四膜虫tubulin蛋白的刺激隐核虫疫苗的制备方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003006480A1 (en) * 2001-07-13 2003-01-23 University Of Rochester Tetrahymena metallothionein gene promoter and its use
WO2003078566A3 (en) * 2002-03-19 2004-03-11 Cilian Ag Dna sequences of major secreted proteins from the ciliate tetrahymena and use thereof
WO2007006812A1 (en) * 2005-07-13 2007-01-18 Cilian Ag Tetrahymena heat inducible promoters and their use

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4752473A (en) 1984-10-12 1988-06-21 The Regents Of The University Of California Expression of glycosylated human influenza hemagglutinin proteins
WO1994020622A1 (en) * 1993-03-11 1994-09-15 Akzo Nobel N.V. Polypeptide fragment capable of inducing neutralising antibodies against feline immuno-deficiency virus
US5762939A (en) 1993-09-13 1998-06-09 Mg-Pmc, Llc Method for producing influenza hemagglutinin multivalent vaccines using baculovirus
AU2756300A (en) 1999-02-04 2000-08-25 University Of Georgia Research Foundation, Inc., The Recombinant expression of heterologous nucleic acids in protozoa
PT1465987E (pt) * 2001-12-07 2008-04-15 Crucell Holland Bv Produção de vírus, isolados virais e vacinas
DE10214406A1 (de) * 2002-03-30 2003-10-09 Nutrinova Gmbh Verfahren und Marker zur einfachen Transformation und Selektion von rekombinanten Protisten
DE10214413A1 (de) * 2002-03-30 2003-10-09 Nutrinova Gmbh Expression von rekombinanten humanen Proteinen in Tetrahymena
AU2006279323B2 (en) * 2005-08-16 2013-08-01 Merck Sharp & Dohme Corp. Influenza recombinant subunit vaccine
US20070286873A1 (en) * 2006-05-23 2007-12-13 Williams John V Recombinant Influenza H5 Hemagluttinin Protein And Nucleic Acid Coding Therefor
WO2009026397A2 (en) * 2007-08-20 2009-02-26 Fraunhofer Usa, Inc. Prophylactic and therapeutic influenza vaccines, antigens, compositions, and methods
GB2471093A (en) 2009-06-17 2010-12-22 Cilian Ag Viral protein expression in ciliates

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003006480A1 (en) * 2001-07-13 2003-01-23 University Of Rochester Tetrahymena metallothionein gene promoter and its use
WO2003078566A3 (en) * 2002-03-19 2004-03-11 Cilian Ag Dna sequences of major secreted proteins from the ciliate tetrahymena and use thereof
WO2007006812A1 (en) * 2005-07-13 2007-01-18 Cilian Ag Tetrahymena heat inducible promoters and their use

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
High efficiency transformation of Tetrahymena using a codon-optimized neomycin resistance gene;MOCHIZUKI ,ET AL;《GENE》;20081201;第425卷(第1-2期);79-83 *

Also Published As

Publication number Publication date
EP2443232A2 (en) 2012-04-25
CN107083393A (zh) 2017-08-22
US8377682B2 (en) 2013-02-19
WO2010146043A2 (en) 2010-12-23
WO2010146043A3 (en) 2011-02-17
ES2627402T3 (es) 2017-07-28
CA2765799A1 (en) 2010-12-23
GB2471093A (en) 2010-12-22
GB0910357D0 (en) 2009-07-29
CN102712909A (zh) 2012-10-03
IN2012DN00448A (https=) 2015-05-15
EP2443232B1 (en) 2017-03-01
PL2443232T3 (pl) 2017-08-31
DK2443232T3 (en) 2017-05-22
JP2012529899A (ja) 2012-11-29
US20110008835A1 (en) 2011-01-13
CA2765799C (en) 2018-04-03

Similar Documents

Publication Publication Date Title
JP7065803B2 (ja) 植物におけるインフルエンザウイルス様粒子の生成
CN101883856B (zh) 包含植物内生产之血凝素的流感病毒样颗粒(vlp)
EP2477650B1 (en) Virus like particles comprising target proteins fused to plant viral coat proteins
US20090117144A1 (en) Recombinant flu vaccines
KR20100120157A (ko) 헤마글루티닌을 발현하는 트랜스제닉 식물에서 생산된 재조합 인플루엔자 바이러스-유사 입자(ＶＬＰｓ)
CN107083393B (zh) 用于在纤毛虫宿主细胞中异源表达病毒蛋白的系统
JP2023539356A (ja) 改変コロナウイルス構造タンパク質
US12467058B2 (en) Influenza virus-like particle production in plants
US20240050559A1 (en) Method of making virus-like particle
CA2907591C (en) Influenza virus-like particle production in plants
Esteban et al. Production of Recombinant HA1 subunit from H5N1 Avian Influenza Hemagglutinin in Escherichia coli

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant