IN2012DN00448A - - Google Patents
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- Publication number
- IN2012DN00448A IN2012DN00448A IN448DEN2012A IN2012DN00448A IN 2012DN00448 A IN2012DN00448 A IN 2012DN00448A IN 448DEN2012 A IN448DEN2012 A IN 448DEN2012A IN 2012DN00448 A IN2012DN00448 A IN 2012DN00448A
- Authority
- IN
- India
- Prior art keywords
- viral protein
- fragment
- host cell
- heterologous expression
- cdna
- Prior art date
Links
- 108010067390 Viral Proteins Proteins 0.000 abstract 3
- 241000223782 Ciliophora Species 0.000 abstract 2
- 239000002299 complementary DNA Substances 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/162—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from virus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
- A61K39/145—Orthomyxoviridae, e.g. influenza virus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
- C07K14/08—RNA viruses
- C07K14/11—Orthomyxoviridae, e.g. influenza virus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16051—Methods of production or purification of viral material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16211—Influenzavirus B, i.e. influenza B virus
- C12N2760/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16211—Influenzavirus B, i.e. influenza B virus
- C12N2760/16234—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/60—Vectors containing traps for, e.g. exons, promoters
Abstract
"System for the heterologous expression of a viral protein in a ciliate host cell" The present invention relates to a system for the heterologous expression of a viral protein or a fragment thereof, said system comprising a) a ciliate host cell, b) at least one cDNA encoding for a viral protein, or a fragment thereof, and c) a promoter operably linked to said cDNA (Fig. 8)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0910357A GB2471093A (en) | 2009-06-17 | 2009-06-17 | Viral protein expression in ciliates |
PCT/EP2010/058360 WO2010146043A2 (en) | 2009-06-17 | 2010-06-15 | System for the heterologous expression of a viral protein in a ciliate host cell |
Publications (1)
Publication Number | Publication Date |
---|---|
IN2012DN00448A true IN2012DN00448A (en) | 2015-05-15 |
Family
ID=40940892
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IN448DEN2012 IN2012DN00448A (en) | 2009-06-17 | 2010-06-15 |
Country Status (11)
Country | Link |
---|---|
US (1) | US8377682B2 (en) |
EP (1) | EP2443232B1 (en) |
JP (1) | JP2012529899A (en) |
CN (2) | CN107083393B (en) |
CA (1) | CA2765799C (en) |
DK (1) | DK2443232T3 (en) |
ES (1) | ES2627402T3 (en) |
GB (1) | GB2471093A (en) |
IN (1) | IN2012DN00448A (en) |
PL (1) | PL2443232T3 (en) |
WO (1) | WO2010146043A2 (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2471093A (en) | 2009-06-17 | 2010-12-22 | Cilian Ag | Viral protein expression in ciliates |
US20160186188A1 (en) * | 2010-02-09 | 2016-06-30 | Rutgers, The State University Of New Jersey | Methods for altering polypeptide expression and solubility |
JP6841184B2 (en) * | 2017-08-07 | 2021-03-10 | 日立金属株式会社 | Manufacturing method of semiconductor devices |
CN108130340B (en) * | 2018-02-06 | 2021-02-23 | 福建省农业科学院畜牧兽医研究所 | Method for expressing duck source avian influenza virus NS1 protein and application of method |
CN114569710A (en) * | 2022-03-09 | 2022-06-03 | 华南农业大学 | Preparation method of Cryptocaryon irritans vaccine based on tetrahymena thermophila tubulin protein |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4752473A (en) | 1984-10-12 | 1988-06-21 | The Regents Of The University Of California | Expression of glycosylated human influenza hemagglutinin proteins |
WO1994020622A1 (en) * | 1993-03-11 | 1994-09-15 | Akzo Nobel N.V. | Polypeptide fragment capable of inducing neutralising antibodies against feline immuno-deficiency virus |
US5762939A (en) | 1993-09-13 | 1998-06-09 | Mg-Pmc, Llc | Method for producing influenza hemagglutinin multivalent vaccines using baculovirus |
EP1151104A1 (en) | 1999-02-04 | 2001-11-07 | University Of Georgia Research Foundation, Inc. | Diagnostic and protective antigen gene sequences of ichthyophthirius |
WO2003048348A2 (en) * | 2001-12-07 | 2003-06-12 | Crucell Holland B.V. | Production of viruses, viral isolates and vaccines |
US20030027192A1 (en) * | 2001-07-13 | 2003-02-06 | Gorovsky Martin A. | Tetrahymena metallothionein gene promoter and its use |
US20060127973A1 (en) * | 2002-03-19 | 2006-06-15 | Marcus Hartmann | Dna sequences of major secreted proteins from the ciliate tetrahymena and use thereof |
DE10214413A1 (en) * | 2002-03-30 | 2003-10-09 | Nutrinova Gmbh | Expression of recombinant human proteins in Tetrahymena |
DE10214406A1 (en) * | 2002-03-30 | 2003-10-09 | Nutrinova Gmbh | Preparation of recombinant protists, useful for preparation of recombinant proteins, by transforming auxotrophic mutant with DNA that complements the auxotrophy |
ATE498690T1 (en) * | 2005-07-13 | 2011-03-15 | Cilian Ag | HEAT-INDUCABLE TETRAHYMENA PROMOTORS AND USE THEREOF |
CA2656705A1 (en) * | 2005-08-16 | 2007-02-22 | Hawaii Biotech, Inc. | Influenza recombinant subunit vaccine |
US20070286873A1 (en) * | 2006-05-23 | 2007-12-13 | Williams John V | Recombinant Influenza H5 Hemagluttinin Protein And Nucleic Acid Coding Therefor |
US20110059130A1 (en) * | 2007-08-20 | 2011-03-10 | Fraunhofer Usa, Inc. | Prophylactic and therapeutic influenza vaccines, antigens, compositions and methods |
GB2471093A (en) | 2009-06-17 | 2010-12-22 | Cilian Ag | Viral protein expression in ciliates |
-
2009
- 2009-06-17 GB GB0910357A patent/GB2471093A/en not_active Withdrawn
-
2010
- 2010-06-15 CA CA2765799A patent/CA2765799C/en active Active
- 2010-06-15 EP EP10724856.9A patent/EP2443232B1/en active Active
- 2010-06-15 WO PCT/EP2010/058360 patent/WO2010146043A2/en active Application Filing
- 2010-06-15 DK DK10724856.9T patent/DK2443232T3/en active
- 2010-06-15 JP JP2012515458A patent/JP2012529899A/en active Pending
- 2010-06-15 PL PL10724856T patent/PL2443232T3/en unknown
- 2010-06-15 IN IN448DEN2012 patent/IN2012DN00448A/en unknown
- 2010-06-15 CN CN201710152797.0A patent/CN107083393B/en active Active
- 2010-06-15 ES ES10724856.9T patent/ES2627402T3/en active Active
- 2010-06-15 CN CN2010800367364A patent/CN102712909A/en active Pending
- 2010-06-16 US US12/816,883 patent/US8377682B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
CA2765799A1 (en) | 2010-12-23 |
US20110008835A1 (en) | 2011-01-13 |
CN102712909A (en) | 2012-10-03 |
CA2765799C (en) | 2018-04-03 |
ES2627402T3 (en) | 2017-07-28 |
CN107083393A (en) | 2017-08-22 |
WO2010146043A3 (en) | 2011-02-17 |
EP2443232A2 (en) | 2012-04-25 |
WO2010146043A2 (en) | 2010-12-23 |
DK2443232T3 (en) | 2017-05-22 |
US8377682B2 (en) | 2013-02-19 |
EP2443232B1 (en) | 2017-03-01 |
JP2012529899A (en) | 2012-11-29 |
CN107083393B (en) | 2021-12-10 |
PL2443232T3 (en) | 2017-08-31 |
GB0910357D0 (en) | 2009-07-29 |
GB2471093A (en) | 2010-12-22 |
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