CN107079626A - It is a kind of by plant three-coloured amaranth reduce soil heavy metal cadmium, lead contamination method - Google Patents
It is a kind of by plant three-coloured amaranth reduce soil heavy metal cadmium, lead contamination method Download PDFInfo
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- CN107079626A CN107079626A CN201710453357.9A CN201710453357A CN107079626A CN 107079626 A CN107079626 A CN 107079626A CN 201710453357 A CN201710453357 A CN 201710453357A CN 107079626 A CN107079626 A CN 107079626A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
- A01H4/006—Encapsulated embryos for plant reproduction, e.g. artificial seeds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Developmental Biology & Embryology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Environmental & Geological Engineering (AREA)
- Health & Medical Sciences (AREA)
- Mechanical Engineering (AREA)
- Processing Of Solid Wastes (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention disclose it is a kind of by plant three-coloured amaranth reduction soil heavy metal cadmium, lead contamination method.The present invention passes through the heavy metal in the root system of three-coloured amaranth and the big Foliage Absorption Enriching soil of three-coloured amaranth, and three-coloured amaranth plant has good landscape effect and solid native effect, also both sides of highway is not suitable for it, containing heavy metal cadmium, lead production and use enterprise, soil remediation containing the emphasis polluted place such as cadmium and lead discarded object, have soil remediation and the double effect of landscaping concurrently, the higher tolerance beyond plant of content of the heavy metal in soil then can not carry out soil remediation using plant, the present invention artificially adds the adverse circumstances of the nutrient solution relative remission plant growth of suitable leaf embryo and callus etc. using artificial seed in seed embeds liquid, the stock for being beneficial to three-coloured amaranth plant reaches the effect of biological prosthetic contaminated soil.
Description
Technical field
The invention belongs to field of environmental improvement, relate to the use of the three-coloured amaranth plant heavy metal pollution of heavy metal accumulation efficiency high
The method that soil is repaired, it is specifically a kind of by plant three-coloured amaranth reduce soil heavy metal cadmium, lead contamination method.
Background technology
In recent years, as population rapid growth, industrial or agricultural are developed rapidly, human activity causes plurality of heavy metal pollutant to enter
Enter soil environment, cause heavy metal pollution of soil increasingly serious.The deterioration of heavy metal pollution seriously threatens national health, a variety of
Taking place frequently for contamination accident, causes the strong worry of various circles of society.The whole world will expend a large amount of manpower and financial resources to administer every year
Heavy metal pollution of soil, in recent years, phytoremediation technology is as a kind of new heavy metal pollution of soil recovery technique, as this
The study hotspot in field.The restorative procedure of traditional heavy-metal contaminated soil has heavy metal pollution of soil, thermal desorption, solidification, soil flushing etc.,
These methods are generally extracted because of investment using green plants and its symbiotic microorganism, transfer, absorb, decompose, conversion or fixed soil
Organic or inorganic pollutant in earth, removes pollutant from soil, so that reach removal, cut down or stable pollutant, or
Reduce the purposes such as pollutant toxicity.But phytoremediation for soil process is slower than physical and chemical process, efficiency is low, and the cycle is long.And
And to select different types of plant for the soil of different contamination class, pollution level.Phytoremediation for soil technology is to soil
The natural conditions such as fertility, weather, moisture, salinity, acid-base value, draining and irrigation system and artificial condition have certain requirement.Plant
Also its repair ability can be influenceed when thing is attacked by pest and disease damage.
Research shows, is acted on by absorption, volatilization, the root filter of plant, degraded, stable etc., can be with the dirt in purification soil
Thing is contaminated, the purpose of environment purification is reached, thus phytoremediation for soil technology is a kind of curbing environmental pollution with development potentiality
Green technology.
The content of the invention
The present invention propose it is a kind of by plant three-coloured amaranth reduce soil heavy metal cadmium, lead contamination method, improve Cadmium in Soil, lead
The remediation efficiency of pollution.Shorten the repair time of contaminated soil, while also acting as the effect beautified the environment.
To achieve the above object, it is of the present invention it is a kind of by plant three-coloured amaranth reduce soil heavy metal cadmium, lead contamination side
Method, by the heavy metal in the root system of three-coloured amaranth and the big Foliage Absorption Enriching soil of three-coloured amaranth, using heavy metal in three-coloured amaranth body
Natural metabolism runs to the cauline leaf part up to ground, by the normal harvesting of three-coloured amaranth, so that the heavy metal pollution in soil is taken away,
Reach the unexpected purpose of repairing heavy metal pollution；It is transferred to concentrate elsewhere after cauline leaf is dried after collection and carries out innoxious place
Reason.
It is of the present invention it is a kind of by plant three-coloured amaranth reduce soil heavy metal cadmium, lead contamination method, including three-coloured amaranth is indefinite
The embedding of bud and the preparation process of artificial seed, when three-coloured amaranth seed length to cotyledonary embryos, take out cotyledonary embryos from culture medium, sterile
It is seeded on cotyledonary embryos growth medium and cultivates；The pH of the cotyledonary embryos growth medium is 5.8, and composition is MS+LKT0.3mg/
The agar of L+NAA2.2mg/L+ glutamic acid 500mg/L+ angelica extract 0.2-0.4mg/L+3.0% brown sugar+0.8%；Condition of culture
For using LED feux rouges as light source, in 35 μm of olm of light intensity-2·s-1Under the conditions of illumination 14h；By sterile artificial endosperm and CaCl2It is molten
Liquid is respectively placed in different beakers；In an aseptic environment, the cotyledonary embryos after the cotyledonary embryos grown cultures basal growth 3 days are put
Enter in the seed embedding liquid, described seed embedding liquid is the sodium alginate hydrosol, and every milliliter of seed, which is embedded, contains 1 in liquid
Cotyledonary embryos；Then one cotyledonary embryos is sucked together with seed embedding liquid with internal diameter 4mm suction pipe, drops to CaCl2In solution,
Per drop volume 0.8ml, after solidification 10min, three-coloured amaranth cotyledonary embryos capsule, as artificial three-coloured amaranth seed are formed.
The sodium alginate hydrosol composition is：
4.0-6.0% sodium alginate+MS+IAA0.4-0.45mg/L+2,4-D0.2-0.25mg/L+3.0-4.0% sucrose+
Bupleurum extract 0.2-0.4mg/L+ Astragalus Root P.Es 0.2-0.4mg/L.
The three-coloured amaranth leaf embryo can also be three-coloured amaranth callus, one kind in three-coloured amaranth adventitious bud or combination.
The angelica extract is the extraction extract of Radix Angelicae Sinensis；Bupleurum extract is that radix bupleuri extracts extract；Astragalus extraction
Thing is that the Radix Astragali extracts extract.
The method of the artificial three-coloured amaranth seed restoration of soil polluted by heavy metal, realizes that step is as follows：
By humic acid fertilizer, stalk fertilizer, fishbone dust with 2:1:After 1 weight is than mixing, heavy metal pollution is uniformly spread on
Soil ploughs deeply 20-25cm behind ground；
Sowing：In the 11-12 months or 2-3 month in and month out samming up to more than 10 DEG C when sow, by between 5cm in the ditch for apply base manure
Put 1 artificial three-coloured amaranth seed or it is dilute be sprinkling upon evenly in ditch, overstocked person, which then picks up, separately puts dilute place's seed treatment；
Fertilising：Can piss element, every mu of each consumption 0.2-0.3kg after height of seedling 3-6cm in the rainy day；After height of seedling 6-8cm,
1 urea, every mu of each consumption 0.1-0.2kg were spread every 17~22 days.
It is described that seed is after planting completed into compacting, pour once permeable, soil moisture content is reached maxmun field capacity
More than 90%.
When the three-coloured amaranth highly reaches more than 10cm, three-coloured amaranth is periodically trimmed, the cauline leaf collection cut collects after drying
Middle carry out harmless treatment.
Described this method is applied to the reparation of heavy-metal contaminated soil, is particularly suitable for use in the reparation of lead-contaminated soil.
It is of the present invention it is a kind of by plant three-coloured amaranth reduce soil heavy metal cadmium, lead contamination method, its advantage exists
In：By the heavy metal in the root system of three-coloured amaranth and the big Foliage Absorption Enriching soil of three-coloured amaranth, and three-coloured amaranth plant has good scape
Effect and solid native effect are seen, is not also suitable for both sides of highway, containing cadmium, lead production and using enterprise, containing cadmium, lead discarded object etc.
The soil remediation of emphasis polluted place, has soil remediation and the double effect of landscaping concurrently, and content of the heavy metal in soil is got over
Height can not then carry out soil remediation beyond the tolerance of plant using plant, and the present invention is artificial in kind of an attached bag using artificial seed
The adverse circumstances for the nutrient solution relative remission plant growth that suitable leaf embryo and callus etc. are added in liquid are buried, are beneficial to three-coloured amaranth
The stock of plant reaches the effect of biological prosthetic contaminated soil.
Embodiment
Embodiment 1
A kind of method of artificial three-coloured amaranth seed restoration of soil polluted by heavy metal of the present invention, realizes that step is as follows：
By humic acid fertilizer, stalk fertilizer, fishbone dust with 2:1:After 1 weight is than mixing, heavy metal pollution is uniformly spread on
Soil ploughs deeply 20cm behind ground；
Sowing：
The preparation of artificial seed：When three-coloured amaranth seed length to cotyledonary embryos, cotyledonary embryos are taken out from culture medium, aseptic inoculation exists
Cultivated on cotyledonary embryos growth medium；The pH of the cotyledonary embryos growth medium is 5.8, and composition is MS+LKT0.3mg/L+
The agar of NAA2.2mg/L+ glutamic acid 500mg/L+ angelica extract 0.25mg/L+3.0% brown sugar+0.8%, angelica extract is
The extraction extract of Radix Angelicae Sinensis；Condition of culture is using LED feux rouges as light source, in 35 μm of olm of light intensity-2·s-1Under the conditions of illumination
14h；By sterile artificial endosperm and CaCl2Solution is respectively placed in different beakers；In an aseptic environment, will be in the cotyledonary embryos
Cotyledonary embryos of the grown cultures basal growth after 3 days are put into the seed embedding liquid, and described seed embedding liquid is sodium alginate water
Colloidal sol；
The sodium alginate hydrosol composition is：
4.5% sodium alginate+MS+IAA0.4mg/L+2,4-D0.2mg/L+3.5% sucrose+bupleurum extract 0.25mg/L
+ Astragalus Root P.E 0.25mg/L, bupleurum extract is that radix bupleuri extracts extract, and Astragalus Root P.E is that the Radix Astragali extracts extract；Often
Contain 1 cotyledonary embryos in milliliter seed embedding liquid；Then one cotyledonary embryos is embedded into liquid one together with seed with internal diameter 4mm suction pipe
Suction is played, CaCl is dropped to2In solution, per drop volume 0.8ml, after solidification 10min, three-coloured amaranth cotyledonary embryos capsule is formed, is artificial
Three-coloured amaranth seed；
In 2 month in and month out samming up to more than 10 DEG C when sow, by putting 1 artificial three-coloured amaranth seed between 5cm in the ditch for apply base manure
Or it is dilute be sprinkling upon evenly in ditch, overstocked person then picks up and separately puts dilute place's seed treatment, described that seed after planting is completed into compacting, pours
It is once permeable, soil moisture content is reached more than the 90% of maxmun field capacity；
Fertilising：Can piss element, every mu of each consumption 0.25kg after height of seedling 3cm in the rainy day；After height of seedling 6cm, every 20
It spreads 1 urea, and every mu of each consumption 0.15kg when three-coloured amaranth highly reaches more than 10cm, is periodically trimmed to three-coloured amaranth, cut
Cauline leaf collect dry after concentrate carry out harmless treatment.
Embodiment 2
A kind of method of artificial three-coloured amaranth seed restoration of soil polluted by heavy metal of the present invention, realizes that step is as follows：
By humic acid fertilizer, stalk fertilizer, fishbone dust with 2:1:After 1 weight is than mixing, heavy metal pollution is uniformly spread on
Soil ploughs deeply 20-25cm behind ground；
Sowing：
The preparation of artificial seed：When three-coloured amaranth seed length to cotyledonary embryos, cotyledonary embryos are taken out from culture medium, aseptic inoculation exists
Cultivated on cotyledonary embryos growth medium；The pH of the cotyledonary embryos growth medium is 5.8, and composition is MS+LKT0.3mg/L+
The agar of NAA2.2mg/L+ glutamic acid 500mg/L+ angelica extract 0.2-0.4mg/L+3.0% brown sugar+0.8%, angelica extract
For the extraction extract of Radix Angelicae Sinensis；Condition of culture is using LED feux rouges as light source, in 35 μm of olm of light intensity-2·s-1Under the conditions of illumination
14h；By sterile artificial endosperm and CaCl2Solution is respectively placed in different beakers；In an aseptic environment, will be in the cotyledonary embryos
Cotyledonary embryos of the grown cultures basal growth after 3 days are put into the seed embedding liquid, and described seed embedding liquid is sodium alginate water
Colloidal sol；
The sodium alginate hydrosol composition is：
4.0% sodium alginate+MS+IAA0.4mg/L+2,4-D0.2mg/L+3.0% sucrose+bupleurum extract 0.2mg/L+
Astragalus Root P.E 0.2mg/L, bupleurum extract is that radix bupleuri extracts extract, and Astragalus Root P.E is that the Radix Astragali extracts extract；Per milli
Rise in seed embedding liquid containing 1 cotyledonary embryos；Then one cotyledonary embryos is embedded into liquid together with seed with internal diameter 4mm suction pipe
Suction, drops to CaCl2In solution, per drop volume 0.8ml, after solidification 10min, three-coloured amaranth cotyledonary embryos capsule, as artificial amaranth are formed
Colza；
In December or 3 month in and month out samming up to more than 10 DEG C when sow, by putting 1 artificial amaranth between 5cm in the ditch for apply base manure
Colza it is sub or it is dilute be sprinkling upon evenly in ditch, overstocked person then picks up and separately puts dilute place's seed treatment, described after planting to complete seed
Compacting, pours once permeable, soil moisture content is reached more than the 90% of maxmun field capacity；
Fertilising：Can piss element, every mu of each consumption 0.2kg after height of seedling 3cm in the rainy day；After height of seedling 6cm, every 22 days
1 urea is spread, every mu of each consumption 0.1kg when three-coloured amaranth highly reaches more than 10cm, is periodically trimmed to three-coloured amaranth, cut
Cauline leaf collection is concentrated after drying and carries out harmless treatment.
Embodiment 3
A kind of method of artificial three-coloured amaranth seed restoration of soil polluted by heavy metal of the present invention, realizes that step is as follows：
By humic acid fertilizer, stalk fertilizer, fishbone dust with 2:1:After 1 weight is than mixing, heavy metal pollution is uniformly spread on
Soil ploughs deeply 20cm behind ground；
Sowing：
The preparation of artificial seed：When three-coloured amaranth seed length to cotyledonary embryos, cotyledonary embryos are taken out from culture medium, aseptic inoculation exists
Cultivated on cotyledonary embryos growth medium；The pH of the cotyledonary embryos growth medium is 5.8, and composition is MS+LKT0.3mg/L+
The agar of NAA2.2mg/L+ glutamic acid 500mg/L+ angelica extract 0.2mg/L+3.0% brown sugar+0.8%, angelica extract is to work as
The extraction extract returned；Condition of culture is using LED feux rouges as light source, in 35 μm of olm of light intensity-2·s-1Under the conditions of illumination 14h；
By sterile artificial endosperm and CaCl2Solution is respectively placed in different beakers；In an aseptic environment, will be in the cotyledon embryonic development
Cultivate cotyledonary embryos of the basal growth after 3 days to be put into the seed embedding liquid, described seed embedding liquid is the sodium alginate hydrosol；
The sodium alginate hydrosol composition is：
6.0% sodium alginate+MS+IAA0.4mg/L+2,4-D0.25mg/L+4.0% sucrose+bupleurum extract 0.4mg/L
+ Astragalus Root P.E 0.4mg/L, bupleurum extract is that radix bupleuri extracts extract, and Astragalus Root P.E is that the Radix Astragali extracts extract；Per milli
Rise in seed embedding liquid containing 1 cotyledonary embryos；Then one cotyledonary embryos is embedded into liquid together with seed with internal diameter 4mm suction pipe
Suction, drops to CaCl2In solution, per drop volume 0.8ml, after solidification 10min, three-coloured amaranth cotyledonary embryos capsule, as artificial amaranth are formed
Colza；
In 11 month in and month out samming up to more than 10 DEG C when sow, by putting 1 artificial three-coloured amaranth seed between 5cm in the ditch for apply base manure
Or it is dilute be sprinkling upon evenly in ditch, overstocked person then picks up and separately puts dilute place's seed treatment, described that seed after planting is completed into compacting, pours
It is once permeable, soil moisture content is reached more than the 90% of maxmun field capacity；
Fertilising：Can piss element, every mu of each consumption 0.3kg after height of seedling 6cm in the rainy day；After height of seedling 8cm, every 22 days
1 urea is spread, every mu of each consumption 0.2kg when three-coloured amaranth highly reaches more than 10cm, is periodically trimmed to three-coloured amaranth, cut
Cauline leaf collection is concentrated after drying and carries out harmless treatment.
4. contrast test：Embodiment 3 is subjected to Soil K+adsorption with not planting the identical contaminated soil of three-coloured amaranth：
From above-mentioned Soil K+adsorption experimental data table, the content of the heavy metal in soil after plantation three-coloured amaranth is all corresponding
Reduce, it is effective to enriching heavy metal, it is effective to the soil of repairing heavy metal pollution.
Claims (8)
1. it is a kind of by plant three-coloured amaranth reduce soil heavy metal cadmium, lead contamination method, include embedding and the people of three-coloured amaranth adventitious bud
The preparation process of work post, it is characterised in that：When three-coloured amaranth seed length to cotyledonary embryos, cotyledonary embryos are taken out from culture medium, it is sterile
It is seeded on cotyledonary embryos growth medium and cultivates；The pH of the cotyledonary embryos growth medium is 5.8, and composition is MS+LKT0.3mg/
The agar of L+NAA2.2mg/L+ glutamic acid 500mg/L+ angelica extract 0.2-0.4mg/L+3.0% brown sugar+0.8%；Condition of culture
For using LED feux rouges as light source, in 35 μm of olm of light intensity-2·s-1Under the conditions of illumination 14h；By sterile artificial endosperm and CaCl2It is molten
Liquid is respectively placed in different beakers；In an aseptic environment, the cotyledonary embryos after the cotyledonary embryos grown cultures basal growth 3 days are put
Enter in the seed embedding liquid, described seed embedding liquid is the sodium alginate hydrosol, and every milliliter of seed, which is embedded, contains 1 in liquid
Cotyledonary embryos；Then one cotyledonary embryos is sucked together with seed embedding liquid with internal diameter 4mm suction pipe, drops to CaCl2In solution,
Per drop volume 0.8ml, after solidification 10min, three-coloured amaranth cotyledonary embryos capsule, as artificial three-coloured amaranth seed are formed.
2. as claimed in claim 1 it is a kind of by plant three-coloured amaranth reduce soil heavy metal cadmium, lead contamination method, its feature exists
In：The sodium alginate hydrosol, composition is 4.0-6.0% sodium alginates+MS+IAA0.4-0.45mg/L+2,4-D0.2-
0.25mg/L+3.0-4.0% sucrose+bupleurum extract 0.2-0.4mg/L+ Astragalus Root P.Es 0.2-0.4mg/L.
3. as claimed in claim 1 it is a kind of by plant three-coloured amaranth reduce soil heavy metal cadmium, lead contamination method, its feature exists
In：The three-coloured amaranth leaf embryo can also be three-coloured amaranth callus, one kind in three-coloured amaranth adventitious bud or combination.
4. as described in any one of claim 1-3 it is a kind of by plant three-coloured amaranth reduce soil heavy metal cadmium, lead contamination side
Method, it is characterised in that：The angelica extract is the extraction extract of Radix Angelicae Sinensis；Bupleurum extract is that radix bupleuri extracts extract；It is yellow
Stilbene extract is that the Radix Astragali extracts extract.
5. a kind of method of artificial three-coloured amaranth seed restoration of soil polluted by heavy metal described in utilization claim 1-4, it is characterised in that：
By humic acid fertilizer, stalk fertilizer, fishbone dust with 2:1:After 1 weight is than mixing, heavy-metal contaminated soil is uniformly spread on
20-25cm is ploughed deeply behind ground；
Sowing：In the 11-12 months or 2-3 month in and month out samming up to more than 10 DEG C when sow, by putting 1 between 5cm in the ditch for apply base manure
The artificial three-coloured amaranth seed of grain or it is dilute be sprinkling upon evenly in ditch, overstocked person, which then picks up, separately puts dilute place's seed treatment；
Fertilising：Can piss element, every mu of each consumption 0.2-0.3kg after height of seedling 3-6cm in the rainy day；After height of seedling 6-8cm, every
Spread within 17~22 days 1 urea, every mu of each consumption 0.1-0.2kg.
6. the method for artificial three-coloured amaranth seed restoration of soil polluted by heavy metal as claimed in claim 5, it is characterised in that：The sowing
Seed is completed into compacting afterwards, pours once permeable, soil moisture content is reached more than the 90% of maxmun field capacity.
7. the method for artificial three-coloured amaranth seed restoration of soil polluted by heavy metal as claimed in claim 6, it is characterised in that：The three-coloured amaranth
When highly reaching more than 10cm, three-coloured amaranth is periodically trimmed, the cauline leaf collection cut is concentrated after drying and carries out harmless treatment.
8. the method for artificial three-coloured amaranth seed restoration of soil polluted by heavy metal as claimed in claim 7, it is characterised in that：The we
Method is particularly suitable for use in the reparation of cadmium and lead-contaminated soil.
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| CN109534895A (en) * | 2018-11-29 | 2019-03-29 | 中国农业科学院棉花研究所 | It is a kind of to administer the base manure auxiliary agent of Cotton Soil lead contamination, base manure and its preparation method and application |
| CN110771507A (en) * | 2019-11-26 | 2020-02-11 | 大连大学 | Preparation method of artificial astragalus membranaceus seeds |
| CN112024584A (en) * | 2020-07-23 | 2020-12-04 | 湖南农业大学 | Cadmium-polluted soil treatment device and method |
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