CN107073095B - 基于脂多糖缺失的细胞组分的抗鲍氏不动杆菌疫苗 - Google Patents

Abstract

本发明涉及一种包含来自不动杆菌属的LPS缺失的灭活细胞和/或由其形成的外膜囊泡的组合物，及其在制备用于预防由不动杆菌属生物体引起的疾病的药物，优选是疫苗，中的用途。

Description

基于脂多糖缺失的细胞组分的抗鲍氏不动杆菌疫苗

技术领域

本发明一般涉及药理学和免疫学领域，并且特别地涉及适于预防性治疗由鲍氏不动杆菌(Acinetobacter baumannii)菌株在哺乳动物中引起的感染的疫苗组合物。

背景技术

由多药耐药鲍氏不动杆菌引起的感染的临床重要性提高确保用于预防和治疗由该病原体引起的感染的新方法的发展。在这种背景下，某些患者群体的免疫可有助于降低由该病原体引起的发病率和死亡率。基于灭活的全细菌细胞的抗革兰氏阴性细菌疫苗具有高度免疫原性，并且已经显示出对许多细菌种类产生保护性免疫。然而，由于脂多糖的存在，这些疫苗中高水平的内毒素使它们在人类中的使用复杂化。

鲍氏不动杆菌是在医院环境中具有临床重要性的革兰氏阴性结肠杆菌。这种生物体高度分布在土壤和周围水源中(Baumann,P.1968.J.Bacteriol.96,39-42)，并且可以作为院内病原体引起不同类型的感染，例如肺炎、菌血症、脑膜炎和皮肤，软组织感染等(García-Quintanilla et al.,2013.Curr.Pharm.Biotechnol.In press)。这种病原体通常感染接受机械通气的患者和身受烧伤的患者(

-Price yWeinstein.2008.N.Engl.J.Med.358,1271-1281)。然而，在社区获得性肺炎的情况下(Hoet al.,2009.Chest.136,1119-1127；Ong et al.,2009.Respirology 14,1200-1205)和在越南、伊拉克、科威特和阿富汗中身受战争相关创伤的军事人员中(Jones et al.,2006.Lancet Infect.Dis.6,317-318；Tong,1972.JAMA 219,1044-1047)也分离出鲍氏不动杆菌。对于医院内感染，与鲍氏不动杆菌感染相关的粗死亡率在35％和70％之间(Vila yPachón,2008.Expert Opin.Pharmacother.9,587-599)。由于鲍氏不动杆菌获得对抗生素的耐药性的能力，多药耐药菌株的数量在前几年显著增加(Tasbakan et al.,2009.Mikrobiyol Bul.43,61-70；Valencia et al.,2009.Infect.ControlHosp.Epidemiol.30:257-263)。这些高耐药性菌株的出现使得由鲍氏不动杆菌引起的感染的临床管理复杂化。在这种背景下，抗鲍氏不动杆菌疫苗的开发可以降低由该病原体引起的发病率和死亡率(Pachón y McConnell,2014.Vaccine,In press)。

已经描述的鲍氏不动杆菌实验疫苗可以分为两大类，由单一纯化抗原组成的疫苗和多组分疫苗。在第一组内，外膜蛋白OmpA(Luo et al.,2012.PLoS One 7,e2944)，生物膜相关蛋白Bap(Fattahian et al.,2011.Microb.Pathog.51,402-406)，膜转运蛋白Ata(Bentancor et al.,2012.Infect.Immun.80,3381-3388)和表面多糖聚-N-乙酰基-β-(1-6)-葡萄糖胺(Bentancor et al.,2012.Infect Immun 80,651-656)由于其引发特异性免疫应答的能力而被描述为良好的候选者。然而，主动免疫后测试存活实验仅证明OmpA提供部分保护，并且在没有形成生物膜的菌株中没有清楚地证明Bap的表达。使用多组分疫苗的方法包括外膜复合物(McConnell et al.,2011.Infect.Immun.79,518-526)，外膜囊泡(outer membrane vesicles；McConnell et al.,2011.Vaccine 29,5705-5710)和灭活的全细胞(McConnell y Pachón,2010.Vaccine 29:1-5)。这些疫苗中的每一种诱导强免疫应答，并且能够在使用ATCC 19606菌株和临床分离物的小鼠模型中引发高水平抗感染保护。然而，尽管这些结果给人以希望，鉴于这些疫苗中高含量的内毒素(因这些制剂中存在大量的脂多糖(LPS))，这些疫苗在人类中的使用是复杂的。

LPS由O抗原、核心多糖和类脂A形成，类脂A负责LPS的内毒素活性。使用大肠杆菌的第一研究证明LPS的产生对细菌活力是必需的(Raetz,1990.Annu.Rev Biochem.59,129-170)。然而，随后的工作表明，某些细菌物种，比如脑膜炎奈瑟氏菌和卡他莫拉菌(Neisseria meningitidis y Moraxella catarrhalis)，在编码参与LPS生物合成的酶的基因突变后是活的，导致菌株完全缺失LPS(Peng et al.,2005.Infect.Immun.73,7569-7577；Steeghs et al.,1999.Infect.Immun.67,4988-4993)。最近的研究表明鲍氏不动杆菌可以通过参与LPS生物合成的基因lpxA、lpxC和lpxD的突变获得对抗生素粘菌素的耐药性(Moffatt et al.,2010.Antimicrob.Agents Chemother.54,4971-4977)，完全缺乏LPS的菌株的存活上升。该结果表明鲍氏不动杆菌在不存在LPS的情况下也可存活，提高了基于这些菌株开发疫苗的可能性。

外膜囊泡(OMV)是由许多革兰氏阴性细菌分泌的来自细菌外膜的囊泡(Kulp etal.,2010.Annu.Rev.Microbiol.64,163-184)。OMV是约20-200nm的球形囊泡，其由外膜蛋白、周质蛋白和LPS组成(Kuehn et al.,2005.Genes Dev.19,2645-2655；Mashburn etal.,2005.Nature.437,422-425)。分泌的OMV已经显示参与群体感应检测，毒力因子转运和基因转移，表明它们在细菌发病机理中起作用。还已经证明，OMV可以通过与脂筏融合将蛋白质递送至宿主细胞内部，表明OMV可以用于大距离运输细菌产物(Kesty et al.,2004.Embo J.23,4538-4549)。Kwon等人最近的研究证明，鲍氏不动杆菌的临床分离物在体外生长期间分泌OMV(Kwon et al.,2009.FEMS Microbiol.Lett.297,150-156)。OMV的蛋白质组学分析证明它们含有多种毒力因子和免疫调节蛋白，这表明OMV在鲍氏不动杆菌的发病机理中起重要作用。

已经开发有基于OMV的疫苗，其用于各种革兰氏阴性细菌，包括脑膜炎奈瑟氏菌、幽门螺杆菌和霍乱弧菌(Bjune et al.,1991.NIPH Ann.14,125-130；Keenan et al.,2003.FEMS Immunol.Med.Microbiol.36,199-205；Bishop et al.,2012.J.Infect.Dis.205,412-21)。用OMV免疫显示会诱导抗多种细菌抗原的抗体，以及在感染的动物模型中提供保护性免疫的能力。此外，分离自脑膜炎奈瑟氏菌血清群B的OMV显示在人类中是安全且致免疫的，并且已经用于控制新西兰脑膜炎球菌性脑膜炎的爆发(Nokleby et al.,2007.Vaccine.25,3080-84)。

由鲍氏不动杆菌引起的感染通常发生在由单个克隆引起的爆发中。为此，如果OMV从致病性克隆中分离，基于OMV的疫苗可能更有效。细菌培养物中OMV的纯化是快速且简单的，仅需要过滤培养物上清液和浓缩OMV。

发明内容

方面A：本发明的该方面涉及适于预防性治疗(感染前)由鲍氏不动杆菌菌株在哺乳动物中引起的感染的药物组合物，优选疫苗组合物，其包括：

a.脂多糖(LPS)缺失的鲍氏不动杆菌菌株全细胞，其特征在于使编码内源性

LPS的一种或多种细胞核酸分子部分或完全失活；和/或

b.来源于上段a)中定义的脂多糖(LPS)缺失的鲍氏不动杆菌菌株的外膜囊泡(OMV)。

在本发明该方面的优选实施例中，脂多糖(LPS)缺失的鲍氏不动杆菌菌株的特征在于选自lpxA、lpxB和/或lpxC的基因的部分或完全失活。

在本发明该方面的另一个优选实施例或其任何优选实施例中，所述药物组合物，优选疫苗组合物，还包含选自以下的多肽，优选重组多肽：

a.氨基酸序列SEQ ID No 27(推定的三价铁铁载体受体(鲍氏不动杆菌ATCC17978；登录号YP_001084684))或其片段，其中术语“片段”在本文中理解为生物活性片段，其选自SEQ ID No 1至SEQ ID NO 11或其任何组合，或与序列SEQ ID NO 1至SEQ ID NO 11中的任一个具有至少85％一致性的氨基酸序列；和/或

b.氨基酸序列SEQ ID No 28(推定的异羟肟酸铁铁载体受体(鲍氏不动杆菌ATCC17978；登录号YP_001084696))或其片段，其中术语片段在本文中理解为生物活性片段，其选自SEQ ID NO 12至SEQ ID NO 23或其任何组合，或与氨基酸SEQ ID NO 12至SEQ ID NO23具有至少85％一致性的序列。

在本发明该方面的另一个优选实施例或其任何优选实施例中，药物组合物，优选疫苗组合物，还包含选自以下的鲍氏不动杆菌的纯化的外膜蛋白序列：SEQ ID NO:29、SEQID NO:30、SEQ ID NO:31、SEQ ID NO:32、SEQ ID NO:33、SEQ ID NO:34、SEQ ID NO:35、SEQID NO:36、SEQ ID NO:37、SEQ ID NO:38、SEQ ID NO:39、SEQ ID NO:40、SEQ ID NO:41、SEQID NO:42、SEQ ID NO:43、SEQ ID NO:44、SEQ ID NO:45、SEQ ID NO:46、SEQ ID NO:47、SEQID NO:48、SEQ ID NO:49、SEQ ID NO:50、SEQ ID NO:51、SEQ ID NO:52、SEQ ID NO:53、SEQID NO:54、SEQ ID NO:55、SEQ ID NO:56、SEQ ID NO:57、SEQ ID NO:58、SEQ ID NO:59、SEQID NO:60、SEQ ID NO:61、SEQ ID NO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ ID NO:65、SEQID NO:66、SEQ ID NO:67、SEQ ID NO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ ID NO:71、SEQID NO:72、SEQ ID NO:73、SEQ ID NO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQID NO:78、SEQ ID NO:79、SEQ ID No:80、SEQ ID NO:81、SEQ ID NO:82、SEQ ID NO:83、SEQID NO:84、SEQ ID NO:85、SEQ ID NO:86、SEQ ID NO:87、SEQ ID NO:88、SEQ ID NO:89或其任何组合。

在本发明该方面的另一个优选实施例或其任何优选实施例中，药物组合物，优选疫苗组合物，还包含融合重组多肽序列SEQ ID NO 24和/或25和/或由核苷酸序列SEQ IDNO 26编码的氨基酸序列。

在本发明该方面的另一个优选实施例或其任何优选实施例中，脂多糖(LPS)缺失的鲍氏不动杆菌菌株包含这样的核苷酸序列或用这样的核苷酸序列转化、转导或转染：能够编码在任何前述段落中定义的任何氨基酸序列，使得所述菌株能够产生任何这些氨基酸序列的外源表达。

在本发明该方面的另一个优选实施例或其任何优选实施例中，所述药物组合物，优选疫苗组合物，还包含载体，例如病毒载体、质粒或表达盒，其包含能够编码任何前述段落中定义的任何氨基酸序列的核苷酸序列并表达所述氨基酸序列。

在本发明该方面的另一个优选实施例或其任何优选实施例中，脂多糖(LPS)缺失的鲍氏不动杆菌菌株是灭活的。优选地，所述菌株或细胞来源于ATCC菌株19606。

方面B：本发明的该方面涉及本发明的方面A或其任何优选实施例的药物组合物，优选疫苗组合物，用于预防性治疗或主动免疫由鲍氏不动杆菌在哺乳动物中，优选在人类中引起的感染。

方面C：本发明的该方面涉及包含抗体(单克隆或多克隆)或其片段的疫苗组合物，抗体(单克隆或多克隆)或其片段优选地选自Fab、Fab'、Fab'-SH、Fv、scFv、F(ab')₂、Vhh、纳米抗体和双抗体，其对脂多糖(LPS)缺失的鲍氏不动杆菌菌株和/或由其衍生的外膜囊泡(OMV)具有亲和力或结合亲和力。在优选的实施例中，所述抗体或其片段特异性结合脂多糖(LPS)缺失的鲍氏不动杆菌菌株和/或由其衍生的外膜囊泡(OMV)。

“亲和力”或“结合亲和力”KD通常通过测量平衡结合常数(ka)和平衡解离常数(kd)并计算kd与ka的商(KD＝kd/ka)来确定。

术语“特异性结合”是指抗体以低于或等于10"⁶M的亲和力KD(单价亲和力)结合缺乏LPS的菌株或由其衍生的OMV。与其它不相关分子相比，该抗体可以对靶抗原具有明显更大的亲和力抗体。与同源物相比，该抗体对靶抗原具有明显更大的亲和力，例如至少1.5倍、2倍、5倍、10倍、100倍、10"³倍、10"⁴倍、10"⁵倍、10"⁶倍或对靶抗原具有更大的相对亲和力。这种亲和力可以使用常规技术轻易地确定，例如，通过平衡透析、通过使用放射性标记的靶抗原的放射免疫测定法，或本领域技术人员知晓的另一方法。亲和力数据可以通过例如[Kaufman RJ,Sharp PA.(1982)Amplification and expression of sequencescotransfected with a modular dihydrofolate reductase complementary dna gene.JMol Biol.159:601-621]中描述的方法进行分析。

术语“抗体”以最广泛的含义使用且包括完全组装的抗体、单克隆抗体、多克隆抗体、多特异性抗体(例如，双特异性抗体)、可结合抗原的抗体片段(例如，Fab'、F'(ab)2、Fv、单链抗体、双抗体)、骆驼抗体和包含前述抗体的重组肽，只要它们显示出所需的生物活性。

因此，抗体/免疫球蛋白的“功能片段”或“抗原结合抗体片段”定义为保留抗原结合区的抗体/免疫球蛋白片段(例如，IgG的可变区)。抗体的“抗原结合区”通常见于抗体的一个或多个高变区，即CDR-1、CDR-2和/或CDR-3区；然而，可变“框架”区也可以在抗原结合中起重要作用，例如通过为CDR提供支架。

术语“高变区”是指负责抗原结合的抗体或功能片段的可变结构域VH和VL的氨基酸残基。抗体片段的非限制性实例包括Fab、Fab'、F(ab')2、Fv，结构域抗体(dAb)、互补决定区(CDR)片段、单链抗体(scFv)、单链抗体片段、双抗体、三链抗体(triabodies)、四链抗体(tetrabodies)、微型抗体(minibodies)、，线性抗体[J Johnson G,Wu TT.(2000)Kabatdatabase and its applications:30years after the first variabilityplot.Nucleic Acids Res.28:214-218]；螯合重组抗体、三体或双体、胞内抗体、纳米抗体、小模块免疫药物(SMI Ps)、抗原结合结构域免疫球蛋白融合蛋白、骆驼化抗体、含VHH的抗体，或突变蛋白或其衍生物，和含至少一部分免疫球蛋白(足以使特异性抗原结合至多肽)的多肽，例如CDR序列，只要该抗体保留所需的生物活性；以及由抗体片段形成的多特异性抗体[Chothia C,Lesk AM.(1987)Canonical structures for the hypervariableregions of immunoglobulins.J Mol Biol.196:901-917；Zapata G,Ridgway JB,Mordenti J,Osaka G,Wong WL,Bennett GL,Carter P.(1995)Engineering linear F(ab')2fragments for efficient production in Escherichia coli and enhancedantiproliferative activity.Protein Eng.8:1057-1062]。

在本发明方面C的优选实施例中，药物或疫苗组合物是用如本发明第一方面定义的疫苗组合物免疫哺乳动物后获得的或可获得的。

方面D：本发明的该方面涉及如本发明的方面C中所定义的疫苗组合物，其用于治疗性处理(感染后)，或用于由鲍氏不动杆菌在哺乳动物，优选在人中，引起的感染的被动免疫。方面E：本发明的该方面涉及脂多糖(LPS)缺失的鲍氏不动杆菌菌株，该脂多糖(LPS)缺失的鲍氏不动杆菌菌株用能够编码如本发明方面A中所定义的任何氨基酸序列的核苷酸序列转化，转导或转染，使得这种菌株能够产生任何所述氨基酸序列的外源表达。优选地，所述鲍氏不动杆菌缺陷菌株用作药物。

方面F：本发明的该方面涉及生产抗体或其片段的方法，所述抗体或其片段优选地选自Fab、Fab'、Fab'-SH、Fv、scFv、F(ab')₂、Vhh、纳米抗体和双抗体，所述方法包括：

a.选择抗体或其片段，优选地选自抗体库；

b.使用脂多糖(LPS)缺失的鲍氏不动杆菌菌株和/或由其衍生的外膜囊泡(OMV)作为抗原靶；

c.选择具有亲和力或结合亲和力或能够特异性结合这种菌株和/或OMV的那些抗体或其片段；并且

d.生产在上述步骤c)中鉴定的那些抗体或其片段

应当注意，鲍氏不动杆菌中的血清型特异性变异依赖于LPS组分的表位的变化。通过根据上述方面F中详述的方法消除LPS组分，我们使抗体集中提升至针对与LPS组分无关的细菌表面变异较少的表位的抗体。这些表位在鲍氏不动杆菌菌株或属于鲍氏不动杆菌分类中使用的所有鲍氏不动杆菌国际克隆的个体分离物中是保守的。因此，该方法提供了用于抗鲍氏不动杆菌感染的普及治疗的工具，其与造成宿主感染的菌株的来源无关。

方面G：本发明的该方面涉及通过本发明方面F的方法产生，获得或可获得的抗体或其片段。方面H：本发明的该方面涉及脂多糖(LPS)缺失和/或由其衍生的外膜囊泡(OMV)的鲍氏不动杆菌菌株用于生产具有亲和力或结合亲和力，或能够特异性结合这种菌株和/或OMV，的抗体或其片段的用途。

发明的具体描述

本发明涉及由鲍氏不动杆菌(A.baumannii)的LPS缺失的全细胞和/或外膜囊泡组成的组合物和疫苗，所述组合物和疫苗能够抗由感染性病原体引起的感染提供保护。

发明人证明，LPS缺失的鲍氏不动杆菌灭活细胞和/或来自鲍氏不动杆菌的外膜囊泡在接种后产生免疫，这为抗由所述细菌引起的后感染提供了保护，证明了这些细胞或菌株作为抗鲍氏不动杆菌引起的感染的预防性疫苗的效用。

本发明的第一方面涉及一种在LPS缺失的鲍氏不动杆菌细胞或菌株，下文称为本发明的细胞或菌株。

不动杆菌的种类是严格好氧的非发酵和氧化酶阴性非运动杆菌，并在显微镜中成对出现。它们在自然界中广泛分布，并且在土壤中是重要的且有助于土壤的矿化。

应当理解，本发明中的“灭活细胞”是不具有复制能力但保持其免疫原性能力的细胞。本发明的细胞在接种之前被灭活以防止它们在宿主中复制，并因此阻止通过其施用产生的发明。本发明的细胞的灭活可以使用现有技术中已知的各种方法进行，例如但不限于：吸附、加热、紫外光、电离辐射、超声、苯酚、甲醛、甲醛、结晶紫、甘油醛、环氧乙烷、丙内酯、乙烯胺(ethylenamina)、溴乙烯胺(bromoethyleneamina)或福尔马林。在优选的实施例中，本发明的细胞用福尔马林灭活。在另一个优选的实施例中，本发明的细胞来自鲍氏不动杆菌并且它们用福尔马林灭活。

在本发明的该方面的一个优选实施例中，LPS缺陷可以通过使编码LPS亚基(特别是LPS的lpxA、lpxB和/或lpxC)的内源基因的一种或多种细胞核酸分子部分或全部失活而实现。

在本发明的另一个优选实施例中，通过在编码参与LPS生物合成的基因的核酸序列和/或控制其表达的序列中缺失和/或插入一个或多个核苷酸而获得LPS缺失的不动杆菌细胞或菌株。缺失和/或插入可以通过同源重组、插入转座子或本领域已知的其它适当方法产生。

在本发明的优选实施例中，通过，例如，构建含有基因lpxA,lpxB,lpxC,lpxD,lpxK,lpxL和/或lpxM或其任何组合的自杀载体，或者以筛选用标记基因打断，用载体转化靶细胞并且筛选LPS表达阴性的阳性细胞，从而使所述序列失活。

在本发明的另一个优选实施例中，本发明的细胞或菌株优选为鲍氏不动杆菌细胞，特别是减毒的鲍氏不动杆菌细胞或其它不动杆菌、贝氏不动杆菌(Acinetobacterbaylyi)、拜叶林克氏不动杆菌(A.beijerinckii)、A.bereziniae、A.boissieri、波氏不动杆菌(A.bouvetii)、A.brisouii、碳酸钙不动杆菌(A.calcoaceticus)、金氏不动杆菌(A.gerneri)、A.guillouiae、格氏不动杆菌(A.grimontii)、A.gyllenbergii、溶血不动杆菌(A.haemolyticus)、A.indicus、约翰逊不动杆菌(A.johnsonii)、琼氏不动杆菌(A.junii)、洛菲不动杆菌(A.lwoffii)、A.nectaris、医院不动杆菌(A.nosocomialis)、小茵落不动杆菌(A.parvus)、皮特不动杆菌(A.pittii)、A.puyangensis、抗辐射不动杆菌(A.radioresistens)、A.rudis、信氏不动杆菌(A.schindleri)、A.soli、坦氏不动杆菌(A.tandoii)、腾氏不动杆菌(A.tjernbergiae)、唐氏不动杆菌(A.towneri)、乌氏不动杆菌(A.ursingii)或威尼斯不动杆菌(A.venetianus)。

在本报告中，应当理解，不动杆菌是指细菌界，变形菌门，γ变形菌纲，假单胞菌目，莫拉氏菌科。

应当理解，本发明中的“鲍氏不动杆菌的细胞或菌株”是属于细菌界，变形菌门，γ变形菌纲，假单胞菌目，莫拉氏菌科，不动杆菌属，鲍氏不动杆菌种的那些细胞。

应当理解，“脂多糖(LPS)或脂寡糖”是在各种革兰氏阴性菌的外膜上发现的组分。由于其发现史，术语LPS通常和“内毒素”可互换使用。LPS由多糖链组成，其余是脂质，称为脂质A，其是形成内毒素活性的原因。多糖链在不同细菌之间是可变的并且决定血清型。内毒素的大小约为10kDa，但可形成高达1000kDa的大聚集体。人类能够生产针对LPS的抗体，但是通常这些抗体只能防御特定血清型的细菌。内毒素是形成由革兰氏阴性菌如脑膜炎奈瑟氏菌和鲍氏不动杆菌引起的感染的许多临床表现的原因。

本发明的组合物

本发明的第二方面涉及一种组合物，下文成为本发明的组合物，其包括：

a)本发明的细胞或菌株，以及

b)任选地，核苷酸和/或氨基酸或多肽序列。

在本发明该方面的更优选的实施例中，核酸和/或氨基酸序列或多肽是重组的。

在另一个更优选的实施例中，所述多肽选自

I)肽序列SEQ ID NO.27(推定的三价铁载体受体(鲍氏不动杆菌ATCC 17978；登录号YP_001084684))或其片段，其中所述片段是生物活性片段，优选地选自SEQ ID NO：

1至SEQ ID NO.11或其任何组合，或与肽序列SEQ ID NO.1至SEQ ID NO.11具有至少85％序列一致性的序列，和/或

II)肽序列SEQ ID No 28(推定的异羟肟酸铁铁载体受体(鲍氏不动杆菌ATCC17978；登录号YP_001084696))或其片段，其中所述片段是生物活性片段，优选地选自SEQID NO：12至SEQ ID NO.23或其任何组合，或与肽序列SEQ ID NO.12至SEQ ID NO.23具有至少85％序列一致性的序列。

在另一个更优选的实施例中，本发明的组合物包含氨基酸序列SEQ ID NO.28和氨基酸序列SEQ ID NO.27。

在另一个优选的实施例中，本发明的组合物包含由选自SEQ ID NO：1至SEQ IDNO：23或者与序列SEQ ID NO：1至SEQ ID NO：23具有至少85％一致性的这些序列的变体的至少2个，优选3个，更优选4个氨基酸序列组成的融合蛋白。

在另一个优选的实施例中，融合蛋白包含氨基酸序列SEQ ID NO：24或氨基酸序列SEQ ID NO：25。

在另一个优选的实施例中，本发明的组合物包含能够转录本发明中所述氨基酸序列的核苷酸序列，下文称为本发明的核苷酸序列。更优选地，核苷酸序列为SEQ ID NO：26。

前述片段在氨基酸序列上至少有一个氨基酸不同。最优选的变体是与SEQ ID NO：1至SEQ ID NO：25中所示的任何多肽具有至少85％或更多，包括90％，93％或更多，并且优选95％或更多，96％或更多，97％或更多，98％或更多，99％或更多序列一致性的那些变体。更优选地，本发明涉及通过与多肽SEQ ID NO：1至SEQ ID NO：25中任一个相关的至少一个(至少两个，至少三个，至少四个)突变表征的序列变体。与发明内容部分中描述的发明一致，突变可以指通过插入、缺失和取代选择的任何突变。优选取代。

在另一个优选的实施例中，本发明的组合物包含表达载体(下文称为本发明的表达载体)，其包含本发明的核苷酸序列。

在另一个优选的实施例中，本发明的组合物还包含LPS缺失的外膜囊泡，下文称为本发明的外膜囊泡。

在另一个优选的实施例中，本发明的组合物还包含从鲍氏不动杆菌的膜纯化的具有氨基酸序列SEQ ID NO:29、SEQ ID NO:30、SEQ ID NO:31、SEQ ID NO:32、SEQ ID NO:33、SEQ ID NO:34、SEQ ID NO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ ID NO:38、SEQ ID NO:39、SEQ ID NO:40、SEQ ID NO:41、SEQ ID NO:42、SEQ ID NO:43、SEQ ID NO:44、SEQ IDNO:45、SEQ ID NO:46；SEQ ID NO:47、SEQ ID NO:48、SEQ ID NO:49、SEQ ID NO:50、SEQ IDNO:51、SEQ ID NO:52、SEQ ID NO:53、SEQ ID NO:54、SEQ ID NO:55、SEQ ID NO:56、SEQ IDNO:57、SEQ ID NO:58、SEQ ID NO:59、SEQ ID NO:60、SEQ ID NO:61、SEQ ID NO:62、SEQ IDNO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ ID NO:66、SEQ ID NO:67、SEQ ID NO:68、SEQ IDNO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ ID NO:72、SEQ ID NO:73、SEQ ID NO:74、SEQ IDNO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ ID NO:78、SEQ ID NO:79、SEQ ID NO:80、SEQ IDNO:81、SEQ ID NO:82、SEQ ID NO:83、SEQ ID NO:84、SEQ ID NO:85、SEQ ID NO:86、SEQ IDNO:87、SEQ ID NO:88，和SEQ ID NO:89或其任何组合的至少一种或蛋白。

更优选地，鲍氏不动杆菌外膜的至少一种蛋白质通过包括以下步骤的方法获得：

a.用含鲍氏不动杆菌ATCC 19606的菌落接种一升Mueller-Hinton肉汤；

b.孵育培养物直到600nm光密度为0.6；

c.用30ml 10mM磷酸盐缓冲液pH7.2洗涤细菌细胞；

d.以6000×g离心10分钟；

e.将细菌沉淀重悬在pH为7.2的10ml 10mM磷酸盐缓冲液中，通过超声处理5次持续1分钟进行裂解；

f.通过以6000xg离心5分钟除去未裂解的细胞；

g.以4℃，20000×g将上清液离心1小时；

h.通过将5ml 2％的N-月桂酰肌氨酸盐溶解在pH7.2的10mM磷酸盐缓冲液中在37℃下持续30分钟，除去外膜的蛋白质；

i.通过在4℃下以20000×g离心1小时沉淀不溶性部分(含有外膜蛋白质)；

j.用pH 6.8的2ml 62.5mM Tric-HCl洗涤沉淀一次，并在4℃下离心1小时；

k.将沉淀重悬在5％SDS溶液中，用甲醇/氯仿沉淀；并且

l.在无菌PBS中重悬沉淀。

在本发明的另一个优选实施例中，本发明的组合物是药物组合物，更优选还包含可接受的药物载体，甚至更优选还包含另一种活性成分。

在另一个优选的实施例中，本发明的组合物还包含佐剂。在另一个优选的实施例中，本发明的组合物为疫苗。

在本发明的上下文中，术语“疫苗”是指用于诱导对疾病的免疫应答的抗原制剂。疫苗由抗原制备，所述抗原一旦在宿主内，通过产生抗体激发免疫应答，并产生免疫记忆，从而产生瞬时或永久免疫。应注意，本文所用的术语“疫苗”还可理解为来自适于由不动杆菌菌株，特别是鲍氏不动杆菌菌株引起的感染的治疗性处理或被动免疫的抗体或其片段的制剂。

核苷酸序列和表达载体

本发明的第三方面涉及核苷酸序列，下文称为本发明的第二核苷酸序列，其编码SEQ ID NO 1至25、27或28中的任一个或其任何组合。本发明的所述第二核苷酸序列还包括核酸序列SEQ ID NO：26。

本发明的第四方面涉及包含本发明的第二核苷酸序列的表达载体，以下称为本发明的表达载体。

在该方面的优选实施例中，本发明的细胞包含本发明的表达载体。

核酸可以位于重组载体中。优选地，重组载体为原核载体，即，含有用于复制和/或整合到原核细胞的基因组中的元件的载体。优选地，重组载体含有可操作地连接表达控制序列的本发明的核酸分子。控制序列优选控制不动杆菌，特别是鲍氏不动杆菌中的活性表达的序列。载体可以是足以整合入染色体的染色体外的载体。本领域的专家已知这样的载体的实例，例如在Sambrook等人的上述文献中所述。

本发明的外膜囊泡

本发明的第五方面涉及LPS缺失的外膜囊泡。在该方面的优选实施例中，外膜囊泡自本发明的细胞或菌株获得。

细胞、囊泡和本发明组合物的用途

第六方面涉及用作药物的本发明的组合物，或者可替换地，涉及本发明的组合物在药物制备上的用途。

本报告中使用的术语“药物”或“药物组合物”是指用于预防、缓解、治疗或治愈人或动物疾病的任何物质。在本发明的上下文中是指包含本发明的组分的组合物。本发明的此组合物包含治疗有效量的不动杆菌属的灭活细胞和/或其外膜囊泡，其能够在针对不动杆菌属而施用该灭活细胞和/或其外膜囊泡的生物体中诱导免疫应答。因此，术语“药物”或“药物组合物”被称为疫苗。

用于获得有效治疗量的剂量取决于多种因素，例如哺乳动物的年龄、体重、性别、耐受性……。本说明书中所使用的“有效治疗量”是指不动杆菌属无活性细胞和/或外膜囊泡产生期望的效果的量，并且通常由期望的治疗效果决定。

本发明的第七方面涉及本发明的组合物，其用于预防，改善或治疗由鲍氏不动杆菌在哺乳动物中引起的感染，或者可替换地，涉及本发明的组合物在加工用于预防，改善或治疗由鲍氏不动杆菌在哺乳动物中引起的感染的药物的用途。

“由不动杆菌属的生物体引起的疾病”应理解为病理病原来自不动杆菌属或其任何代谢产物的那些疾病。不动杆菌属产生各种病理，例如，但不限于：菌血症、脑膜炎、尿路感染、皮肤和软组织感染、手术部位感染和肺炎。由于这些原因，由不动杆菌属生物体引起的疾病的较优选的形式之一选自菌血症、脑膜炎、尿路感染、皮肤和软组织感染、手术部位感染和肺炎。

本发明的药物和组合物可以单独使用或与用于治疗由来自不动杆菌属生物体引起的疾病的其它药物或组合物组合使用。

本发明的药物和组合物也可以包括药学上可接受的载体或赋形剂。

本发明的药物和组合物可以单独使用或与用于治疗或预防由不动杆菌属生物体引起的疾病的其它药物或组合物组合使用。

术语“赋形剂”指的是这样的物质：有助于吸收本发明的药物的组合物的元素，稳定所述元件，活化或帮助制备药物，从而提供稠度或风味，使其更可口。在不排除本段中所述的其它类型的赋形剂的情况下，赋形剂可以将成分保持在一起，例如，为淀粉、糖、纤维素、甜味剂、着色剂的情况，保护药物的功能，例如，将药物与空气和/或湿气隔离，填充丸子、胶囊或任何其它呈递形式的功能，例如，磷酸二氢钙的情况，促进组分的溶解及其在肠中的吸收的功能。

以与赋形剂相同的方式，载体是在药物中用于将本发明的任何组分稀释至期望体积或重量的物质。药学上可接受的载体是惰性物质或与本发明的任何元素具有相似作用的惰性物质。载体的功能是便于结合其它元件，允许更好地配量和施用并且为药物提供稠度和形式。当呈递形式是液体时，药学上可接受的载体是稀释剂。

可用于本发明组合物中的佐剂和药学上可接受的载体是本领域技术人员已知的那些载体。在本报告中，术语“佐剂”是指本身不造成抗原活性的任何试剂，其可用于刺激免疫系统以增加对疫苗的应答。存在许多佐剂，例如，但不限于：磷酸铝、氢氧化铝、toll样受体激动剂、细胞因子、鲨烯、弗氏不完全佐剂和完全佐剂。在本发明的该方面的优选形式中，佐剂选自磷酸铝、氢氧化铝、toll样受体激动剂、细胞因子、鲨烯、弗氏不完全佐剂和完全佐剂。在本发明的该方面的甚至更优选的形式中，佐剂为磷酸铝。

本文所使用的术语“活性成分”、“活性物质”或“药物活性物质”或“药物活性成分”是指在疾病的诊断、治愈、缓解、治疗或预防中潜在地提供药理活性或其它不同作用或影响人或动物体的结构或功能的任何组分。该术语包括在药物加工中促进化学变化，并且以提供特定活性或效果的相同和修饰形式呈递的的那些组分。

本发明的第八方面涉及针对鲍氏不动杆菌在哺乳动物中引起的感染提供保护的本发明的组合物，或者可替换地，涉及本发明的组合物在加工针对鲍氏不动杆菌在哺乳动物中引起的感染提供保护的药物上的用途。

本发明的另一方面涉及本发明的融合蛋白或肽或本发明的第一或第二方面的组合物或本发明的药物组合物，其可以施用一次或多次，例如两次、三次、四次、五次、六次、七次、八次、九次或十次或更多次。每剂中活性成分的量没有特别限制。

本发明的另一方面涉及组合物，其由能够结合至SEQ ID NO：27或SEQ ID NO：28的抗体或其片或在第二方面中定义的融合蛋白或本发明的融合蛋白段组成，其中优选地所述组合物为药物组合物，优选疫苗，并且其中所述药物组合物用于治疗或预防鲍氏不动杆菌引起的感染。

本发明的第九方面涉及可通过用本发明的第一或第二方面的组合物或用本发明的融合蛋白免疫哺乳动物而获得的抗体或其活性片段，优选地所述抗体或活性片段包括这样的组合物，其中优选所述组合物为药物组合物并且所述药物组合物用作治疗方案，特别是用于治疗由鲍氏不动杆菌引起的感染。

根据本发明制备不动杆菌细胞的方法

本发明的第十方面涉及一种制备如上所述的鲍氏不动杆菌细胞的方法。

不动杆菌

与此方面一致，该方法包括以下步骤：(i)提供LPS缺失的细菌细胞，特别是不动杆菌细胞；(ii)在所述细菌细胞中插入重组核酸分子，所述核酸分子编码的融合肽包括以下结构域(a)多肽的至少一个结构域，其中所述结构域能够在哺乳动物中产生免疫应答，和(b)吞噬溶酶体的逃脱结构域；以及(iii)在适当的条件下培养获得的与步骤(ii)一致的细胞。优选地，获得能够表达所述核酸的细胞。更优选地，该细胞为鲍氏不动杆菌细胞。

与另一方面一致，该方法包括以下步骤：(i)提供LPS缺失的细菌细胞，特别是不动杆菌细胞，(ii)在所述细菌中插入重组核酸分子，所述核酸分子编码用于逃脱吞噬溶酶体的肽或多肽，以及(iii)在适当的条件下培养获得的与步骤(ii)一致的细胞。

如果需要，本发明的方法包括在细菌细胞中插入至少一种重组核酸分子，所述分子编码能够在哺乳动物中产生免疫应答的肽或多肽。

应当理解，本发明中的“感染”是由不动杆菌属任何生物体，优选是鲍氏不动杆菌的任何生物体侵袭或定殖任何宿主组织所产生的病状。

应当理解，本发明中的“软组织”是生物体的所有非骨组织。

本发明中所理解的术语“预防”包括避免损伤的出现，所述损伤的原因是不动杆菌属细胞或其任何衍生物或代谢产物。

报告中的术语“抗原”是指可以诱导抗体形成的分子(通常是蛋白质或多糖)。有许多不同类型的分子可以作为抗原，例如蛋白质、肽、多糖，以及更少见的其它分子例如核酸。

术语“耐药性”是指细菌针对药物产生的任何防御机制。由细菌获得和传播的耐药性的机制是最重要的，并且主要包括：产生灭活抗生素的酶，阻碍药物到达其靶标的修饰的出现和/或靶标本身的改变。一种细菌菌株可以针对一种或多种抗生素产生各种耐药性机制，并且抗生素可以以相同的方式被来自各种细菌物种的不同的机制灭活。

术语“多核苷酸”和“核酸”可互换使用，指任何长度的核苷酸的聚合形式，包括DNA和RNA。

术语“氨基酸序列”、“肽”、“寡肽”、“多肽”和“蛋白质”可互换使用，并且是指可以以化学方法或生物化学方法修饰的任何长度的氨基酸的聚合形式。

在权利要求的描述张，词语“包括”及其变体不意在排除其它技术特征，添加物、组分或步骤。对于本领域的专家来说，本发明的描述和实践部分公开了本发明的其它目的、优点和特征。提供以下实施例和附图作为说明，其并不意在限制本发明。

附图说明

图1.IB010的稳定性和内毒素含量。(A)从ATCC 19606和IB010的三份独立培养物提取基因组DNA，并使用lpxD基因特异引物扩增。对应于约1000Kb的条带与完整的lpxD基因对应，而较快的迁移条带与缺失462个核苷酸的lpxD基因对应。(B)通过鲎变形细胞试验(Limulus Amebocyte Assay)测定的ATCC 19606和IB010的内毒素水平。柱代表三份独立培养物的中值，误差条代表平均值的标准误差。EU：内毒素单位。

图2.使用IB010免疫的抗体应答。在免疫前(第0天)和第一次免疫后第7和21天从ATCC 19606免疫小鼠、IB010免疫小鼠和对照小鼠收集血清样本，并通过ELISA测量抗原特异性总IgG(A)和IgM(B)的水平(n＝8只小鼠/组)。在21天测量IgG1(C)和IgG2c(D)水平，在ATCC 19606免疫小鼠、IB010免疫小鼠和对照小鼠中通过ELISA测量血清。在所有图中，盒须图分别表示四分位数范围和范围，水平线表示中值。*与相同时间点对照小鼠中的水平相比，p<0.05，与来自相同实验组的7天样本相比，#p<0.05，与ATCC 19606免疫小鼠中的21天样本相比，

图3.免疫对组织细菌负荷的影响。用2.0×10⁶cfu(300x LD₅₀)的ATCC 19606菌株感染免疫小鼠和对照小鼠，并在感染后12小时确定脾细菌负荷(n＝8只小鼠/组)。数据点代表来自个体小鼠的细菌负荷，水平线代表来自小鼠组的中值。*与对照小鼠相比，p<0.05。

图4.免疫对感染后促炎细胞因子水平的影响。用2.0x 10⁶cfu(300x LD₅₀)的ATCC19606菌株感染免疫小鼠和对照小鼠，确定IL-1β、TNF-α和IL-6的血清水平(n＝8只小鼠/组)。数据点代表来自个体小鼠的细胞因子水平，并且水平线代表来自小鼠组的中值。与对照小鼠相比，*p<0.05，与ATCC 19606免疫小鼠相比，#p<0.05。

图5.免疫对弥散性鲍氏不动杆菌感染的小鼠模型中的存活的影响。用2.25x10⁶cfu(340.9x LD₅₀)的ATCC 19606菌株(A)或1.05x 10⁶cfu(2.18x LD₅₀)的鲍氏不动杆菌临床分离物Ab-154(B)感染免疫小鼠和对照小鼠，在接下来的7天监测存活(n＝8只小鼠/组)。*与对照小鼠相比，p<0.05。

图6.没有LPS的鲍氏不动杆菌OMV的蛋白质图谱。在培养24小时、48小时和72小时后菌株ATCC 19606和IB010用于产生OMV。在纯化OMV后，使用布Bradford方法定量蛋白质的量，并在10％聚丙烯酰胺凝胶中用考马斯染色使10mcg蛋白质显现。

图7.2,2-联吡啶、铁螯合剂对OMV的蛋白质图谱的影响。菌株ATCC 19606和IB010用于分析培养24小时后2,2-联吡啶(BIP)对OMV的蛋白质图谱的影响。在ATCC 19606菌株的情况下，使用200mcM的浓度，而在IB010的情况下使用100mcM和150mcM的浓度。纯化OMV后，使用Bradford方法定量蛋白质的量，并在10％聚丙烯酰胺凝胶中用考马斯染色使10mcg的蛋白质显现。

图8.2,2联吡啶、铁螯合剂对OMV的生产的影响。菌株ATCC 19606和IB010用于分析培养24小时后2,2联吡啶(BIP)对OMV的生产的影响。在该图中，在用BIP处理并用Bradford测量浓度后显示OMV的总蛋白质含量。

图9.OMV的可视化。使用1.6％的戊二醛固定从ATCC 19606纯化的OMV，用四氧化锇和铅及铀染色，并通过电子显微镜显现。

图10.纯化的OMV的可视化。使用1.6％的戊二醛固定从IB010纯化的OMV，四氧化锇和铅及铀染色，并通过电子显微镜显现。

图11.不含LPS的OMV的蛋白质图谱。菌株IB010和167R用于生产OMV，并且定量每个样本的蛋白质的量。在10％聚丙烯酰胺凝胶中用考马斯染色使10mcg的蛋白质显现。

本发明的实施例

实施例1

道德声明

涉及使用动物的所有实验由大学医院伦理和实验委员会批准(niversityHospital Virgen del Rocío Committee on Ethics and Experimentation，评估代码：2013PI/296)。在所有实验中，努力使痛苦最小化，并且在实验过程中使用硫喷妥钠使看起来濒死的任何动物立即安乐死。

细菌菌株。鲍氏不动杆菌ATCC19606是一种对抗生素敏感的参照菌株。如先前所述(临床和实验室标准协会2013)，通过将ATCC 19606的过夜培养物在含有10mg/l粘菌素的Mueller Hinton琼脂上铺板，获得ATCC 19606的LPS缺陷型衍生物。通过对37℃过夜生长后存在的粘菌素耐药突变体的lpxA、lpxC和lpxD基因进行测序，鉴定在涉及LPS生物合成的基因中有突变的菌株。鉴定了lpxD基因中缺失较大的菌株，并命名为IB010。根据临床和实验室标准协会指南[23]，通过肉汤微量稀释来确认对粘菌素的耐药性。使用QCL-1000鲎变形细胞试验(Lonza)，根据制造商的说明书测量每种菌株的三份独立培养物的内毒素水平来证实LPS不存在。Ab-154菌株是先前表征的鲍氏不动杆菌临床分离物(Gautom,1997.J.Clin.Microbiol.35,2977-2980)。

疫苗制备和小鼠免疫。基于先前描述的方法(Moffatt et al.,2010.Antimicrob.Agents Chemother.54,4971-4977)制备IWC疫苗(含LPS和LPS缺陷型)。简而言之，ATCC19606和IB010菌株在Mueller-Hinton肉汤中生长至OD₆₀₀为0.8。在IB010的情况下，向培养物中加入10μg/ml粘菌素。为了证实在IB010生长期间没有发生野生型回复，使ATCC 19606和IB010的三份独立培养物生长，并使用QIAmp DNAMini Kit(Qiagen)从每份培养物中分离基因组DNA。使用lpxD特异性引物5′GCTAATTGGTGAAGGTAGTC 3′和5′GACGAATCGTTTGAATCTGC 3′来从培养物中扩增基因组DNA，以证实在生长后IB010中lpxD缺失的存在。

对于疫苗制备，在磷酸盐缓冲液中彻底洗涤细菌，然后在0.5M福尔马林中在室温下摇动18小时进行灭活。通过在血琼脂上铺板来确认细菌完全灭活。将灭活细胞的浓度调节至1x10¹⁰个细胞/ml，并与基于铝的佐剂，Alhydrogel 2％(w/v)(InvivoGen)以1:1(v/v)组合。通过在第0和14天向每只四头肌肌内注射100μl疫苗在6至8周龄的雌性C57BL/6小鼠中进行免疫。对照小鼠类似地注射磷酸盐缓冲液和佐剂的混合物。

鲍氏不动杆菌感染的小鼠模型。将先前由我们小组开发并用于评价抗鲍氏不动杆菌的疫苗的败血症小鼠模型用于表征功效(Batson et al.,1950.J.Exp.Med.91,219-229；Rodríguez-Hernández et al.,2000.J.Antimicrob.Chemother.45,493-501)。在腹膜内灌注接种物后，该模型产生弥散性感染，通常导致在24至48小时内死亡。为了制备接种物，鲍氏不动杆菌菌株在37℃下在Mueller-Hinton肉汤培养中生长18小时，并如先前所述(Moffatt et al.,2010.Antimicrob.Agents Chemother.54,4971-4977；Martin et al.,1998.J.Immunol.Methods 212,187-192)在生理盐水中调节到适当的浓度。通过在血琼脂上铺板确定接种物的细菌浓度。在第21天(第二次免疫后一周)通过腹膜内注射0.5ml细菌悬浮液感染小鼠，并监测存活7天。

脾细菌负荷和血清细胞因子水平。在感染后12小时，在免疫小鼠和对照小鼠中确定感染后细菌负荷。用过量的硫喷妥钠使小鼠安乐死，在从眶后窦收集血液样本后，无菌取出脾脏，称重并在2ml生理盐水中进行匀浆。将系列对数稀释液在血琼脂平板上铺板，以进行细菌定量。使用BD OptEIA小鼠试剂盒(BD Biosciences)在感染后12小时确定小鼠白细胞介素-1β(IL-1β)、肿瘤坏死因子α(TNF-α)和白细胞介素-6(IL-6)的血清水平。

酶联免疫吸附试验(ELISA)。对于间接酶联免疫吸附试验(ELISA)，通过在4℃下孵育过夜，用含5x 10⁷个细菌细胞/孔的磷酸盐缓冲液涂布96孔板。如先前所述[28]，使用在第0、7和21天收集的血清进行ELISA。针对用于免疫小鼠的菌株测量抗体滴度，并且定义为分光光度计读数比背景孔(不含血清的孔)高至少0.1个单位的稀释度。

统计分析。对独立样本使用Kruskal-Wallis H检验和Mann-Whitney U检验，对相依样本使用Friedmann和Wilcoxon检验，比较抗体滴度、细菌负荷和细胞因子水平。适当时应用Bonferroni校正。使用对数秩检验比较存活数据。所有统计均使用15.0版SPSS软件(SPSS Inc.)进行，p值≤0.05被认为是显著的。

结果

选择LPS缺失的菌株用于疫苗开发。在存在10μg/ml粘菌素的情况下，ATCC 19606的生长导致lpxA、lpxC和lpxD基因突变的许多粘菌素耐药衍生物(数据未显示)。这些菌株之一，IB010，在lpxD(核苷酸104-565)基因中包含462个核苷酸的较大缺失，并被选择在疫苗研究中进一步使用。我们推断，基于该菌株含有较大缺失，与在LPS生物合成基因中包含单个核苷酸变化或较小缺失的菌株相比，该菌株在生长期间不太可能回复为野生型。肉汤微量稀释实验证明ATCC 19606菌株的最小抑制浓度为≤0.25μg/ml，并且对于IB010，最小抑制浓度>128μg/ml。这证明：与前述结果类似，lpxD中的突变可导致对粘菌素的耐药性(Moffatt et al.,2010.Antimicrob.Agents Chemother.54,4971-4977)。为了确保IB010在生长期间的遗传稳定性，来自ATCC 19606和IB010的三份独立培养物的基因组DNA用lpxD特异性引物扩增以证实缺失存在。如图1A所示，从所有IB010培养物扩增后，存在与包含462个核苷酸缺失的IB010的突变lpxD基因的条带，表明没有发生回复。对于ATCC 19606和IB010通过鲎变形细胞试验来表征LPS的表型缺失和内毒素水平的降低，并且证明lpxD基因中的突变导致内毒素水平显著降低至每10⁶个细胞>1EU(图1B)。

对LPS缺失的IWC疫苗的抗体应答。用福尔马林处理ATCC 19606和IB010导致没有活细菌，表明细菌被完全灭活。为了定量通过用灭活的IB010免疫产生的抗体应答，使用从阴性对照小鼠(用PBS和佐剂免疫)和用1x 10⁹个灭活的IB010细胞免疫的小鼠收集的血清，进行间接ELISA。基于我们先前已经显示用这些细胞免疫会诱导强烈的免疫应答并产生抗实验感染的保护性免疫(McConnell y Pachón,2010.Vaccine 29,1-5)，作为阳性对照，小鼠组用1x 10⁹个灭活的ATCC 19606细胞免疫。如图2A所示，用灭活的IB010免疫在单次肌内给药后7天，在所有小鼠中引起可检测的抗原特异性总IgG水平，并且在通过第二次施用疫苗进行加强后，这些抗体水平明显提高(p＝0.03，Wicoxon试验)。接受施用两次灭活的IB010疫苗的小鼠的总IgG滴度与接受含有灭活的野生型细胞的疫苗的小鼠中的滴度相似(p＝0.726Mann Whitney U检验)。对照小鼠在任何点都没有可检测的抗原特异性IgG。相比之下，在单次施用后7天，用灭活的IB010疫苗免疫的小鼠和接受灭活的野生型细胞的小鼠之间的IgM水平相似(p＝0.186Mann Whitney U检验)，然而在第二次免疫后7天，IB010免疫小鼠中没有可检测的抗原特异性IgM，而用灭活的野生型细胞免疫的所有小鼠都具有可检测的IgM水平(图2B)。

确定21天血清中的IgG亚型IgG1和IgG2c(C57BL/6中的IgG2a同源物)的水平(Martin et al.,1998.J.Immunol.Methods 212:187-192)(图2C和D)。与对照小鼠相比，接受灭活疫苗的两组小鼠都具有显著的IgG1和IgG2c水平(p<0.001；Mann-Whitney U检验)。有趣的是，与ATCC 19606免疫小鼠相比，IB010免疫小鼠中的IgG1滴度明显更高(p＝0.003；Mann-Whitney U检验)，而IgG2c滴度在这些组之间相似。这些结果表明，Th1和Th2应答均由灭活的IB010疫苗引起，类似于对于灭活的ATCC 19606疫苗先前所显示的应答(McConnelly Pachón,2010.Vaccine 29,1-5)。

免疫对感染后细菌负荷的影响。为了表征疫苗对感染后组织细菌负载的影响，我们使用由我们组先前开发的小鼠模型表征用于预防鲍氏不动杆菌引起的感染的疫苗(McConnell et al.,2011.Infect.Immun.79,518-526；McConnell et al.,2011.Vaccine29:5705-5710；McConnell y Pachón,2010.Vaccine 29,1-5)。该模型快速产生弥散性感染，其中感染后刚1小时就在远端器官中检测到细菌[16]。用2.0x10⁶ cfu(300x LD₅₀)的ATCC 19606菌株感染免疫小鼠和对照小鼠，并且在感染后12小时测定脾脏细菌负荷(图3)。与对照小鼠相比，IB010免疫使脾脏中的细菌数量降低约1000倍(p<0.05；Mann-Whitney U检验)。IB010免疫小鼠中的脾脏细菌负荷与用灭活的ATCC 19606细胞免疫的小鼠中的细菌负荷没有明显差异。

免疫对感染后血清细胞因子水平和存活的影响。为了表征用灭活的LPS缺陷疫苗免疫对细胞因子水平的影响，在感染后12小时从免疫小鼠和对照小鼠收集血清，并确定IL-1β、IL-6和TNF-α的水平(图4)。两组免疫小鼠的所有三种细胞因子水平明显低于对照小鼠的所有三种细胞因子水平(对于IL-1β、IL-6和TNF-α，p＝0.003；Mann-Whitney U检验)，表明免疫小鼠没有经历与败血性休克的发展相关的促炎细胞因子释放。

在第二次免疫后7天，通过用2.25x 10⁶cfu(340.9x LD₅₀)的ATCC 19606菌株感染免疫小鼠和对照小鼠来测试疫苗功效，并监测存活七天(图5)。用IB010疫苗免疫的所有小鼠都免于攻击，而所有对照小鼠在48小时内死亡(P<0.001；对数秩检验)。如预期，用ATCC19606菌株免疫的所有小鼠都在攻击中存活，类似于先前报道的结果(McConnell et al.,2011.Infect.Immun.79,518-526；McConnell et al.,2011.Vaccine 29:5705-5710；McConnell y Pachón,2010.Vaccine 29,1-5)。为了确定用IB010免疫是否可以保护免于被不相关的菌株进行异源攻击，用1.05x 10⁶cfu(2.18x LD₅₀)以前表征的鲍氏不动杆菌临床分离物Ab-154感染免疫小鼠和对照小鼠[29]。再次，所有免疫小鼠在攻击下存活，而对照小鼠在48小时内死于感染(p<0.001；对数秩检验)，表明用IB010免疫可针对异源菌株攻击提供的交叉保护。总之，这些结果提供了关于基于缺乏LPS的全细菌细胞开发用于预防由鲍氏不动杆菌引起的感染的疫苗的重要信息。这些结果还可以提供对基于缺乏LPS的菌株开发用于其它细菌物种的疫苗的可能性的见解。

实施例2

该实施例涉及基于从不含LPS的突变体的所述培养物纯化的OMVS的抗鲍氏不动杆菌的疫苗的开发。

为了实现该目的，菌株ATCC 19606T及其不含LPS的突变体IB010，IB010在我们的实验室中由ATCC 19606T菌株产生，并且包含在基因lpxD的位置103和565之间的462个核苷酸缺失。

在实现所述菌株的OMV的纯化时，使用以下方案：

-使菌株在具有10mcg/ml的粘菌素的血琼脂或MHBII平板上更新，并在37℃下生长过夜。

-液体培养物用于使ATCC 19606或IB010生长。将它们以37℃，180rpm通气培养过夜。

-第二天在MHB中制成50或100ml或1L培养物，并以37℃，通气(180rpm)孵育过夜，

-孵育后，将细胞以4℃，4000rpm离心30分钟。

-接下来，用0.22微米过滤器过滤上清液。

-然后，通过以4℃，以30000rpm超速离心90分钟来沉淀OMV。

最后，将沉淀物重悬浮于PBS中，通过铺板证实不存在活细菌。将OMV存储在-80℃。

(方案改编自McConnell MJ et al.2011Aug 5；29(34):5705-10)

使用先前的方案，对OMV进行了不同的纯化。

-纯化在培养24、48和72小时后产生OMV的菌株ATCC 19606和IB010的OMV。在纯化OMV后，使用Bradford方法定量蛋白质的量，并在10％聚丙烯酰胺凝胶中用考马斯染色使10mcg的蛋白质显现。

此外，在存在和不存在作为铁螯合剂的Bip的情况下，用来自菌株ATCC 19606和IB010的OMV进行纯化，目的是验证螯合剂的存在是否导致与铁代谢相关的蛋白质，例如铁载体受体，的表达增加。在这种情况下，纯化OMV，进行蛋白质定量和丙烯酰胺凝胶的考马斯染色，且通过电子显微镜使OMV显现。

最后，从鲍氏不动杆菌Ab-167的临床分离物的LPS突变体纯化OMV，鲍氏不动杆菌Ab-167在lpxC基因中含有ISAba1插入。将蛋白质定量并通过考马斯染色在丙烯酰胺凝胶上显现。并且通过Bradford和2D Quant试剂盒定量蛋白质。

实施例3.外膜蛋白质的纯化。

鲍氏不动杆菌ATCC19606在1升Mueller-Hinton肉汤中生长至600nm的光密度(OD600)为0.6，将沉淀的细菌重悬于10ml 10mM pH7.2的磷酸盐缓冲液中，并通过声波降解法裂解。通过以4,000x g离心5分钟除去未裂解的细胞，并将上清液以20,000_g离心1小时以沉淀细胞被膜。通过在37℃下孵育30分钟，用5ml的2％N-月桂酰肌氨酸盐选择性地溶解内膜。通过以20,000_g离心1小时使不溶性碎片沉淀，然后用2ml 62.5mM Tris-Cl(pH 6.8)洗涤。

通过使用冷清洗剂洗涤步骤从制剂中提取内毒素，其中将蛋白质重悬于5％SDS中并在4℃下孵育10分钟。随后通过在甲醇氯仿中沉淀来除去SDS和内毒素，并重悬浮于PBS中。

佐剂的添加

将浓度为500mcg/ml的纯化蛋白质与磷酸铝佐剂以1:1比例混合。

条款

1.一种LPS缺失的不动杆菌细胞。

2.根据前面权利要求所述的不动杆菌细胞，其通过使编码内源LPS生物合成基因的一个或多个核酸部分或完全失活而获得。

3.根据前面权利要求所述的不动杆菌细胞，其中所述基因选自lpxA、lpxB和/或lpxC或其任何组合。

4.根据前面权利要求中任一项所述的不动杆菌细胞，其中通过在所述基因的编码序列中缺失和/或插入一个或多个核苷酸而获得所述细胞。

5.根据前面权利要求中任一项所述的不动杆菌细胞，其中所述细胞是减毒的不动杆菌细胞。

6.一种组合物，其包含：

a)根据权利要求1-2中任一项的细胞，和

b)核酸分子，和/或多肽。

7.根据前面权利要求所述的组合物，其中所述核酸分子是重组的，并且所述多肽是重组的。

8.根据权利要求6-7中任一项所述的组合物，其中所述多肽选自：

a)肽序列SEQ ID NO：27(推定的三价铁载体受体(鲍氏不动杆菌ATCC 17978；登录号YP_001084684))或其片段，其中所述片段是生物活性片段，并且优选地选自SEQ ID NO：1至SEQ ID NO：11或其任何组合，或与肽序列SEQ ID NO：1至SEQ ID NO：11具有至少85％序列一致性的序列，和/或

b)肽序列SEQ ID No 28(推定的异羟肟酸铁铁载体受体(鲍氏不动杆菌ATCC17978；登录号YP_001084696))或其片段，其中所述片段是生物活性片段，优选地选自SEQID NO：12至SEQ ID NO：23或其任何组合，或与肽序列SEQ ID NO：12至SEQ ID NO：23具有至少85％序列一致性的序列。

9.根据权利要求6-8中任一项所述的组合物，其还包含氨基酸序列SEQ ID NO：28和氨基酸序列SEQ ID NO：27。

10.根据权利要求6-9中任一项所述的组合物，其还包含融合蛋白，所述融合蛋白包含来自SEQ ID NO：1至SEQ ID NO：23，或与SEQ ID NO：1至SEQ ID NO：23具有至少85％的序列一致性的这些序列的变体的至少2个，优选3个，更优选4个氨基酸序列。

11.根据权利要求10所述的组合物，其中所述融合蛋白还包含氨基酸序列SEQ IDNO：24或氨基酸序列SEQ ID NO：25。

12.根据权利要求6-11中任一项所述的组合物，其中所述组合物包含能够转录根据权利要求8-11中任一项所述的氨基酸序列的核苷酸序列。

13.根据权利要求12所述的组合物，其中所述核苷酸序列为SEQ ID NO：26。

14.根据权利要求6-13中任一项所述的组合物，其还包含表达载体，所述表达载体包含根据权利要求12-13中任一项所述的核苷酸序列

15.根据权利要求6-14中任一项所述的组合物，其还包含LPS缺失的外膜囊泡或根据权利要求1至5中任一项所述的细胞。

16.根据权利要求6-14中任一项所述的组合物，其还包含具有氨基酸序列SEQ IDNO:29、SEQ ID NO:30、SEQ ID NO:31、SEQ ID NO:32、SEQ ID NO:33、SEQ ID NO:34、SEQ IDNO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ ID NO:38、SEQ ID NO:39、SEQ ID NO:40、SEQ IDNO:41、SEQ ID NO:42、SEQ ID NO:43、SEQ ID NO:44、SEQ ID NO:45、SEQ ID NO:46、SEQ IDNO:47、SEQ ID NO:48、SEQ ID NO:49、SEQ ID NO:50、SEQ ID NO:51、SEQ ID NO:52、SEQ IDNO:53、SEQ ID NO:54、SEQ ID NO:55、SEQ ID NO:56、SEQ ID NO:57、SEQ ID NO:58、SEQ IDNO:59、SEQ ID NO:60、SEQ ID NO:61、SEQ ID NO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ IDNO:65、SEQ ID NO:66、SEQ ID NO:67、SEQ ID NO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ IDNO:71、SEQ ID NO:72、SEQ ID NO:73、SEQ ID NO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ IDNO:77、SEQ ID NO:78、SEQ ID NO:79、SEQ ID NO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ IDNO:83、SEQ ID NO:84、SEQ ID NO:85、SEQ ID NO:86、SEQ ID NO:87、SEQ ID NO:88、SEQ IDNO:89或其组合的，鲍氏不动杆菌的纯化的至少一种外膜蛋白。

17.一种LPS缺失的外膜囊泡。

18.根据权利要求17所述的外膜囊泡，其从根据权利要求1-5中任一项所述的细胞获得。

19.根据权利要求6-15中任一项所述的组合物，其中所述细胞或所述外膜囊泡设计为生产根据权利要求8-11或16中任一项所述的氨基酸序列和/或包含根据权利要求12-13中任一项所述的核苷酸或核酸。

20.一种核苷酸序列，其能够转录根据权利要求10-11中任一项所述的任何融合蛋白。

21.一种表达载体，其包含根据权利要求12-13中任一项所述的核苷酸或核酸。

22.根据权利要求7至16和19中任一项所述的组合物，所述组合物是药物组合物。

23.根据权利要求22所述的组合物，其还包含药学上可接受的载体。

24.根据权利要求22或23中任一项所述的组合物，其还包含另一种活性成分。

25.根据权利要求22-24中任一项所述的组合物，其还包含佐剂。

26.根据权利要求7至16、19至22和25中任一项所述的组合物，其中所述组合物为疫苗。

27.根据权利要求7至16、19至22和25中任一项所述的组合物，其用作药物。

28.根据权利要求7至16、19至22和25中任一项所述的组合物，其用于预防、改善或治疗由鲍氏不动杆菌在哺乳动物中引起的感染。

29.根据权利要求7至16、19至22和25中任一项所述的组合物，其用于针对鲍氏不动杆菌在哺乳动物中引起的感染提供保护作用。

30.一种抗体或其活性片段，其通过用根据权利要求7至16、19至22和25中任一项所述的组合物免疫哺乳动物获得。

31.根据前面权利要求所述的抗体或其片段，其中所述组合物是药物组合物，并且其中所述组合物用于治疗，特别是用于治疗和预防由鲍氏不动杆菌引起的感染。

附加条款

1.一种LPS缺失的不动杆菌细胞，其通过使编码内源性LPS生物合成基因的一种或多种细胞核酸分子部分或完全失活获得。

2.根据前面权利要求所述的不动杆菌细胞，其中所述基因选自lpxA、lpxB和/或lpxC，或其任何组合。

3.一种组合物，其包含：

a)根据权利要求1-2中任一项所述的细胞，和

b)重组核酸分子，和/或重组多肽。

4.根据权利要求3所述的组合物，其中所述多肽选自：

a)肽序列SEQ ID NO：27(推定的三价铁载体受体(鲍氏不动杆菌ATCC 17978；登录号YP_001084684))或其片段，其中所述片段是生物活性片段，并且优选地选自SEQ ID NO：1至SEQ ID NO：11或其任何组合，或与肽序列SEQ ID NO：1至SEQ ID NO：11具有至少85％序列一致性的序列，和/或

b)肽序列SEQ ID No 28(推定的异羟肟酸铁铁载体受体(鲍氏不动杆菌ATCC17978；登录号YP_001084696))或其片段，其中所述片段是生物活性片段，并且优选地选自SEQ ID NO：12至SEQ ID NO：23或其任何组合，或与肽序列SEQ ID NO：12至SEQ ID NO：23具有至少85％序列一致性的序列。

5.根据权利要求3-4中任一项所述的组合物，其中所述组合物包含能够转录根据权利要求4所述的氨基酸序列的核苷酸序列，优选SEQ ID NO：26。

6.根据权利要求3-5中任一项所述的组合物，还包含LPS缺失的外膜囊泡或根据权利要求1和2中任一项所述的细胞。

7.根据权利要求3-6中任一项所述的组合物，其还包含从鲍氏不动杆菌的外膜纯化的至少一种蛋白质SEQ ID NO:29,SEQ ID NO:30,SEQ ID NO:31,SEQ ID NO:32,SEQ IDNO:33,SEQ ID NO:34,SEQ ID NO:35,SEQ ID NO:36,SEQ ID NO:37,SEQ ID NO:38,SEQ IDNO:39,SEQ ID NO:40,SEQ ID NO:41,SEQ ID NO:42,SEQ ID NO:43,SEQ ID NO:44,SEQ IDNO:45,SEQ ID NO:46；SEQ ID NO:47；SEQ ID NO:48；SEQ ID NO:49；SEQ ID NO:50；SEQ IDNO:51；SEQ ID NO:52；SEQ ID NO:53；SEQ ID NO:54；SEQ ID NO:55；SEQ ID NO:56；SEQ IDNO:57；SEQ ID NO:58；SEQ ID NO:59；SEQ ID NO:60；SEQ ID NO:61；SEQ ID NO:62；SEQ IDNO:63；SEQ ID NO:64；SEQ ID NO:65；SEQ ID NO:66；SEQ ID NO:67；SEQ ID NO:68；SEQ IDNO:69；SEQ ID NO:70；SEQ ID NO:71；SEQ ID NO:72；SEQ ID NO:73；SEQ ID NO:74；SEQ IDNO:75；SEQ ID NO:76,SEQ ID NO:77,SEQ ID NO:78,SEQ ID NO:79,SEQ ID NO:80,SEQ IDNO:81,SEQ ID NO:82,SEQ ID NO:83,SEQ ID NO:84,SEQ ID NO:85,SEQ ID NO:86,SEQ IDNO:87,SEQ ID NO:88,SEQ ID NO:89，或其组合。

8.一种LPS缺失的外膜囊泡。

9.根据权利要求8所述的外膜囊泡，其由根据权利要求1-2中任一项所述的细胞获得。

10.根据权利要求3-7中任一项所述的组合物，其中所述细胞或所述外膜囊泡设计为生产根据权利要求4和7中任一项所述的多肽序列，和/或包含权利要求5所述的核酸序列。

11.根据权利要求3-7和10中任一项所述的组合物，其中所述组合物是药物组合物。

12.根据权利要求3-7、10和11中任一项所述的组合物，其用作药物。

13.根据权利要求3-7、10和11中任一项所述的组合物，其用于预防、改善或治疗由鲍氏不动杆菌在哺乳动物中引起的感染。

14.一种抗体或其活性片段，其通过用根据权利要求3至7、10和11中任一项所述的组合物免疫哺乳动物获得。

15.根据前面权利要求所述的抗体或其活性片段，其中所述组合物为药物组合物，并且其中所述组合物用于治疗，特别是用于治疗和预防由鲍氏不动杆菌引起的感染。

序列表

<110> 安达卢西亚健康服务部

塞维利亚安达卢西亚健康研究管理公共基金会

瓦克斯丁公司

<120> 基于脂多糖缺失的细胞组分的抗鲍氏不动杆菌疫苗

<130> EP-06839

<140> EP14382164.3

<141> 04.07.2014

<160> 101

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<222> (1)..(23)

<223> Anti-A. Baumannii immunogen

<400> 8

Asn Thr Ser Asp Lys Thr Val Gln Phe Asn Asn Arg Ala Ala Lys Val

1 5 10 15

Val Asp Thr Asp Gln Arg Val

20

<210> 9

<211> 22

<212> PRT

<213> Acinetobacter baumannii

<220>

<221> PEPTIDE

<222> (1)..(22)

<223> Anti-A. Baumannii immunogen

<400> 9

Thr Arg Gly Gln Tyr Lys Asp Val Ala Asn Lys Trp His Glu Leu Asn

1 5 10 15

Ser Phe Thr Val Ala Pro

20

<210> 10

<211> 36

<212> PRT

<213> Acinetobacter baumannii

<220>

<221> PEPTIDE

<222> (1)..(36)

<223> Anti-A. Baumannii immunogen

<400> 10

Ile Lys Gly Thr Asn Lys Ala Tyr Lys Asp Asp Arg Glu Leu Ala Ala

1 5 10 15

Phe Ala Thr Thr Gln Asp Glu Ala Phe Gln Asn Ala Val Lys Asn Asp

20 25 30

Ala Asn Ser Ala

35

<210> 11

<211> 33

<212> PRT

<213> Acinetobacter baumannii

<220>

<221> PEPTIDE

<222> (1)..(33)

<223> Anti-A. Baumannii immunogen

<400> 11

Tyr Asn Val Trp Asn Arg Gln Tyr Arg Thr Val Phe Ala Gln Gln Ala

1 5 10 15

Ala Val Ser Asn Ala Asn Pro Leu Leu Ala Ile Pro Ala Glu Gly Arg

20 25 30

Thr

<210> 12

<211> 28

<212> PRT

<213> Acinetobacter baumannii

<220>

<221> PEPTIDE

<222> (1)..(28)

<223> Anti-A. Baumannii immunogen

<400> 12

Val Lys Gly Ala Asp Ser Leu Thr Asn Ala Phe Gly Asp Pro Ser Ala

1 5 10 15

Thr Ile Asn Asn Ile Arg Lys Arg Pro Thr Gln Glu

20 25

<210> 13

<211> 28

<212> PRT

<213> Acinetobacter baumannii

<220>

<221> PEPTIDE

<222> (1)..(28)

<223> Anti-A. Baumannii immunogen

<400> 13

Ser Gly Lys Val Arg Gly Arg Ile Met Gly Tyr Glu Gln Thr Gly Asp

1 5 10 15

Ser Tyr Leu Asp Arg Tyr Ser Ala Glu Lys Asn Gly

20 25

<210> 14

<211> 11

<212> PRT

<213> Acinetobacter baumannii

<220>

<221> PEPTIDE

<222> (1)..(11)

<223> Anti-A. Baumannii immunogen

<400> 14

Ser Gln Glu Gln Asn Lys Pro Asn Ala Asn Asn

1 5 10

<210> 15

<211> 15

<212> PRT

<213> Acinetobacter baumannii

<220>

<221> PEPTIDE

<222> (1)..(15)

<223> Anti-A. Baumannii immunogen

<400> 15

Asn Pro Asn Pro Asp Trp Ala His Trp Asp Asn Glu Thr Gln Asn

1 5 10 15

<210> 16

<211> 36

<212> PRT

<213> Acinetobacter baumannii

<220>

<221> PEPTIDE

<222> (1)..(36)

<223> Anti-A. Baumannii immunogen

<400> 16

Leu Asp Thr Lys His Asn Ser Arg Leu Leu Tyr Tyr Tyr Gly Tyr Pro

1 5 10 15

Lys Ala Asp Gly Ser Gly Val Ser Leu Thr Pro Trp Gly Gly Gln Glu

20 25 30

His Gln Glu Lys

35

<210> 17

<211> 50

<212> PRT

<213> Acinetobacter baumannii

<220>

<221> PEPTIDE

<222> (1)..(50)

<223> Anti-A. Baumanii immunogen

<400> 17

Val Arg Asn His Gln Gln Asp Lys Gln Ser Thr Gly Thr Ile Asn Asp

1 5 10 15

Ser Asn Val Ile Lys Ser Thr Thr Thr Asp Trp Ala Ser Trp Thr Pro

20 25 30

Gln Ser Ile Thr Trp Ser Asp Phe Thr Glu Ala Ala Asn Tyr Lys Gln

35 40 45

Asn Ile

50

<210> 18

<211> 20

<212> PRT

<213> Acinetobacter baumannii

<220>

<221> PEPTIDE

<222> (1)..(20)

<223> Anti-A. Baumannii immunogen

<400> 18

Val Gln Ala Glu Ser Lys Gly Glu Ser Tyr Ser Ser Pro Met Ser Tyr

1 5 10 15

Ser Glu Ser Lys

20

<210> 19

<211> 21

<212> PRT

<213> Acinetobacter baumannii

<220>

<221> PEPTIDE

<222> (1)..(21)

<223> Anti-A. Baumannii immunogen

<400> 19

Arg Pro Gln Thr Gly Ile Asp Lys Asp Thr Asn Gln Ala Leu Lys Pro

1 5 10 15

Ile Glu Gly Lys Ser

20

<210> 20

<211> 29

<212> PRT

<213> Acinetobacter baumannii

<220>

<221> PEPTIDE

<222> (1)..(29)

<223> Anti-A. Baumannii immunogen

<400> 20

Thr Glu Gln Asn Asn Tyr Pro Leu Arg Asn Ser Asp Gly Asn Pro Leu

1 5 10 15

Asn Arg Lys Val Pro Thr Ser Asp Leu Glu Ser Gln Gly

20 25

<210> 21

<211> 20

<212> PRT

<213> Acinetobacter baumannii

<220>

<221> PEPTIDE

<222> (1)..(20)

<223> Anti-A. Baumannii immunogen

<400> 21

Ala Gln Phe Ser Ile Lys Asp Thr Lys Asn Gly Gly Glu Ala Arg Thr

1 5 10 15

Tyr Asn Pro Asn

20

<210> 22

<211> 20

<212> PRT

<213> Acinetobacter baumannii

<220>

<221> PEPTIDE

<222> (1)..(20)

<223> Anti-A. Baumannii immunogen

<400> 22

Gln Asp Gly Ile Lys Leu Tyr Asp Ser Asn Val Asn Gly Thr Ile Lys

1 5 10 15

Gln Asp Ala Tyr

20

<210> 23

<211> 20

<212> PRT

<213> Acinetobacter baumannii

<220>

<221> PEPTIDE

<222> (1)..(20)

<223> Anti-A. Baumannii immunogen

<400> 23

Asp Lys Lys Tyr Leu Asn Ser Phe Pro Asp Gly Gln Ala Phe Tyr Gly

1 5 10 15

Ala Pro Ala Asn

20

<210> 24

<211> 586

<212> PRT

<213> Artificial Sequence

<220>

<223> Fusion protein

<220>

<221> PEPTIDE

<222> (1)..(586)

<223> Fusion protein

<400> 24

Val Lys Gly Ala Asp Ser Leu Thr Asn Ala Phe Gly Asp Pro Ser Ala

1 5 10 15

Thr Ile Asn Asn Ile Arg Lys Arg Pro Thr Gln Glu Ser Gln Glu Gln

20 25 30

Asn Lys Pro Asn Ala Asn Asn Asn Pro Asn Pro Asp Trp Ala His Trp

35 40 45

Asp Asn Glu Thr Gln Asn Leu Asp Thr Lys His Asn Ser Arg Leu Leu

50 55 60

Tyr Tyr Tyr Gly Tyr Pro Lys Ala Asp Gly Ser Gly Val Ser Leu Thr

65 70 75 80

Pro Trp Gly Gly Gln Glu His Gln Glu Lys Val Arg Asn His Gln Gln

85 90 95

Asp Lys Gln Ser Thr Gly Thr Ile Asn Asp Ser Asn Val Ile Lys Ser

100 105 110

Thr Thr Thr Asp Trp Ala Ser Trp Thr Pro Gln Ser Ile Thr Trp Ser

115 120 125

Asp Phe Thr Glu Ala Ala Asn Tyr Lys Gln Asn Ile Val Gln Ala Glu

130 135 140

Ser Lys Gly Glu Ser Tyr Ser Ser Pro Met Ser Tyr Ser Glu Ser Lys

145 150 155 160

Arg Pro Gln Thr Gly Ile Asp Lys Asp Thr Asn Gln Ala Leu Lys Pro

165 170 175

Ile Glu Gly Lys Ser Thr Glu Gln Asn Asn Tyr Pro Leu Arg Asn Ser

180 185 190

Asp Gly Asn Pro Leu Asn Arg Lys Val Pro Thr Ser Asp Leu Glu Ser

195 200 205

Gln Gly Ala Gln Phe Ser Ile Lys Asp Thr Lys Asn Gly Gly Glu Ala

210 215 220

Arg Thr Tyr Asn Pro Asn Gln Asp Gly Ile Lys Leu Tyr Asp Ser Asn

225 230 235 240

Val Asn Gly Thr Ile Lys Gln Asp Ala Tyr Asp Lys Lys Tyr Leu Asn

245 250 255

Ser Phe Pro Asp Gly Gln Ala Phe Tyr Gly Ala Pro Ala Asn Thr Ser

260 265 270

Ser Asp Thr Phe Arg Ser Asp Gly Leu Ala Thr Ser Arg Gly Ser Gln

275 280 285

Phe Asp Gly Asn Gly Asp Arg Ile Ser Leu Ser Pro Trp Gln Gly Ser

290 295 300

Thr Met Asp Thr Asp Thr Tyr Lys Asp Lys Gln Asp Thr Asp Tyr Gly

305 310 315 320

Pro Asp Tyr Ser Tyr Leu Pro Thr Thr Ser Lys Ser Asn Asp Ala Thr

325 330 335

Thr Pro Thr Tyr Lys Ala Ile Lys Gly Leu Lys Leu Ser Asn Pro Leu

340 345 350

Arg Asn Glu Lys Ser Arg Phe Phe Pro Tyr Gly Leu Ser Asn Lys Ser

355 360 365

Val Thr Ser Val Asn Gln Ser Gln Ser Glu Ile Glu Val Glu Lys Asp

370 375 380

Lys Gln Phe Val Asp Ile Leu Ala Thr Gln Tyr Pro Tyr Leu Val Tyr

385 390 395 400

Thr Pro Thr Gly Gln Arg Lys Gly Tyr Gly Pro Asn Thr Glu Ile Gln

405 410 415

Asn Ile Gln Ala Asp Thr Asp Ala Tyr Ile Pro Ser Arg Glu Thr Thr

420 425 430

Met Val Pro Ala Gly Ser Thr His Asp Asp Lys Pro Leu Pro Asp Val

435 440 445

Gln Arg Met Leu Arg Asp Val Ser Thr Tyr Thr Val Ser Thr Ala Asn

450 455 460

Leu Gln Pro Ile Thr Val Asn Ser Asn Thr Ser Asp Lys Thr Val Gln

465 470 475 480

Phe Asn Asn Arg Ala Ala Lys Val Val Asp Thr Asp Gln Arg Val Thr

485 490 495

Arg Gly Gln Tyr Lys Asp Val Ala Asn Lys Trp His Glu Leu Asn Ser

500 505 510

Phe Thr Val Ala Pro Ile Lys Gly Thr Asn Lys Ala Tyr Lys Asp Asp

515 520 525

Arg Glu Leu Ala Ala Phe Ala Thr Thr Gln Asp Glu Ala Phe Gln Asn

530 535 540

Ala Val Lys Asn Asp Ala Asn Ser Ala Tyr Asn Val Trp Asn Arg Gln

545 550 555 560

Tyr Arg Thr Val Phe Ala Gln Gln Ala Ala Val Ser Asn Ala Asn Pro

565 570 575

Leu Leu Ala Ile Pro Ala Glu Gly Arg Thr

580 585

<210> 25

<211> 615

<212> PRT

<213> Artificial Sequence

<220>

<223> Fusion protein

<220>

<221> PEPTIDE

<222> (1)..(615)

<223> Fusion protein

<400> 25

Met Ser Gly Lys Val Arg Gly Arg Ile Met Gly Tyr Glu Gln Thr Gly

1 5 10 15

Asp Ser Tyr Leu Asp Arg Tyr Ser Ala Glu Lys Asn Gly Val Lys Gly

20 25 30

Ala Asp Ser Leu Thr Asn Ala Phe Gly Asp Pro Ser Ala Thr Ile Asn

35 40 45

Asn Ile Arg Lys Arg Pro Thr Gln Glu Ser Gln Glu Gln Asn Lys Pro

50 55 60

Asn Ala Asn Asn Asn Pro Asn Pro Asp Trp Ala His Trp Asp Asn Glu

65 70 75 80

Thr Gln Asn Leu Asp Thr Lys His Asn Ser Arg Leu Leu Tyr Tyr Tyr

85 90 95

Gly Tyr Pro Lys Ala Asp Gly Ser Gly Val Ser Leu Thr Pro Trp Gly

100 105 110

Gly Gln Glu His Gln Glu Lys Val Arg Asn His Gln Gln Asp Lys Gln

115 120 125

Ser Thr Gly Thr Ile Asn Asp Ser Asn Val Ile Lys Ser Thr Thr Thr

130 135 140

Asp Trp Ala Ser Trp Thr Pro Gln Ser Ile Thr Trp Ser Asp Phe Thr

145 150 155 160

Glu Ala Ala Asn Tyr Lys Gln Asn Ile Val Gln Ala Glu Ser Lys Gly

165 170 175

Glu Ser Tyr Ser Ser Pro Met Ser Tyr Ser Glu Ser Lys Arg Pro Gln

180 185 190

Thr Gly Ile Asp Lys Asp Thr Asn Gln Ala Leu Lys Pro Ile Glu Gly

195 200 205

Lys Ser Thr Glu Gln Asn Asn Tyr Pro Leu Arg Asn Ser Asp Gly Asn

210 215 220

Pro Leu Asn Arg Lys Val Pro Thr Ser Asp Leu Glu Ser Gln Gly Ala

225 230 235 240

Gln Phe Ser Ile Lys Asp Thr Lys Asn Gly Gly Glu Ala Arg Thr Tyr

245 250 255

Asn Pro Asn Gln Asp Gly Ile Lys Leu Tyr Asp Ser Asn Val Asn Gly

260 265 270

Thr Ile Lys Gln Asp Ala Tyr Asp Lys Lys Tyr Leu Asn Ser Phe Pro

275 280 285

Asp Gly Gln Ala Phe Tyr Gly Ala Pro Ala Asn Thr Ser Ser Asp Thr

290 295 300

Phe Arg Ser Asp Gly Leu Ala Thr Ser Arg Gly Ser Gln Phe Asp Gly

305 310 315 320

Asn Gly Asp Arg Ile Ser Leu Ser Pro Trp Gln Gly Ser Thr Met Asp

325 330 335

Thr Asp Thr Tyr Lys Asp Lys Gln Asp Thr Asp Tyr Gly Pro Asp Tyr

340 345 350

Ser Tyr Leu Pro Thr Thr Ser Lys Ser Asn Asp Ala Thr Thr Pro Thr

355 360 365

Tyr Lys Ala Ile Lys Gly Leu Lys Leu Ser Asn Pro Leu Arg Asn Glu

370 375 380

Lys Ser Arg Phe Phe Pro Tyr Gly Leu Ser Asn Lys Ser Val Thr Ser

385 390 395 400

Val Asn Gln Ser Gln Ser Glu Ile Glu Val Glu Lys Asp Lys Gln Phe

405 410 415

Val Asp Ile Leu Ala Thr Gln Tyr Pro Tyr Leu Val Tyr Thr Pro Thr

420 425 430

Gly Gln Arg Lys Gly Tyr Gly Pro Asn Thr Glu Ile Gln Asn Ile Gln

435 440 445

Ala Asp Thr Asp Ala Tyr Ile Pro Ser Arg Glu Thr Thr Met Val Pro

450 455 460

Ala Gly Ser Thr His Asp Asp Lys Pro Leu Pro Asp Val Gln Arg Met

465 470 475 480

Leu Arg Asp Val Ser Thr Tyr Thr Val Ser Thr Ala Asn Leu Gln Pro

485 490 495

Ile Thr Val Asn Ser Asn Thr Ser Asp Lys Thr Val Gln Phe Asn Asn

500 505 510

Arg Ala Ala Lys Val Val Asp Thr Asp Gln Arg Val Thr Arg Gly Gln

515 520 525

Tyr Lys Asp Val Ala Asn Lys Trp His Glu Leu Asn Ser Phe Thr Val

530 535 540

Ala Pro Ile Lys Gly Thr Asn Lys Ala Tyr Lys Asp Asp Arg Glu Leu

545 550 555 560

Ala Ala Phe Ala Thr Thr Gln Asp Glu Ala Phe Gln Asn Ala Val Lys

565 570 575

Asn Asp Ala Asn Ser Ala Tyr Asn Val Trp Asn Arg Gln Tyr Arg Thr

580 585 590

Val Phe Ala Gln Gln Ala Ala Val Ser Asn Ala Asn Pro Leu Leu Ala

595 600 605

Ile Pro Ala Glu Gly Arg Thr

610 615

<210> 26

<211> 1650

<212> DNA

<213> Artificial Sequence

<220>

<223> Polynucleotide coding for the fusion protein

<220>

<221> misc_feature

<222> (1)..(1650)

<223> Polynucleotide coding for fusion protein

<400> 26

atgtctggta aagttcgtgg tcgtatcatg ggttacgaac agaccggtga ctcttacctg 60

gaccgttact ctgctgaaaa aaacggtgtt aaaggtgctg actctctgac caacgctttc 120

ggtgacccgt ctgctaccat caacaacatc cgtaaacgtc cgacccagga atctcaggaa 180

cagaacaaac cgaacgctaa caacaacccg aacccggact gggctcactg ggacaacgaa 240

acccagaacc tggacaccaa acacaactct cgtctgctgt actactacgg ttacccgaaa 300

gctgacggtt ctggtgtttc tctgaccccg tggggtggtc aggaacacca ggaaaaagtt 360

cgtaaccacc agcaggacaa acagtctacc ggtaccatca acgactctaa cgttatcaaa 420

tctaccacca ccgactgggc ttcttggacc ccgcagtcta tcacctggtc tgacttcacc 480

gaagctgcta actacaaaca gaacatcgtt caggctgaat ctaaaggtga atcttactct 540

tctccgatgt cttactctga atctaaacgt ccgcagaccg gtatcgacaa agacaccaac 600

caggctctga aaccgatcga aggtaaatct accgaacaga acaactaccc gctgcgtaac 660

tctgacggta acccgctgaa ccgtaaagtt ccgacctctg acctggaatc tcagggtgct 720

cagttctcta tcaaagacac caaaaacggt ggtgaagctc gtacctacaa cccgaaccag 780

gacggtatca aactgtacga ctctaacgtt aacggtacca tcaaacagga cgcttacgac 840

aaaaaatacc tgaactcttt cccggacggt caggctttct acggtgctcc ggctaacacc 900

tcttctgaca ccttccgttc tgacggtctg gctacctctc gtggttctca gttcgacggt 960

aacggtgacc gtatctctct gtctccgtgg cagggttcta ccatggacac cgacacctac 1020

aaagacaaac aggacaccga ctacggtccg gactactctt acctgccgac cacctctaaa 1080

tctaacgacg ctaccacccc gacctacaaa gctatcaaag gtctgaaact gtctaacccg 1140

ctgcgtaacg aaaaatctcg tttcttcccg tacggtctgt ctaacaaatc tgttacctct 1200

gttaaccagt ctcagtctga aatcgaagtt gaaaaagaca aacagttcgt tgacatcctg 1260

gctacccagt acccgtacct ggtttacacc ccgaccggtc agcgtaaagg ttacggtccg 1320

aacaccgaaa tccagaacat ccaggctgac accgacgctt acatcccgtc tcgtgaaacc 1380

accatggttc cggctggttc tacccacgac gacaaaccgc tgccggacgt tcagcgtatg 1440

ctgcgtgacg tttctaccta caccgtttct accgctaacc tgcagccgat caccgttaac 1500

tctaacacct ctgacaaaac cgttcagttc aacaaccgtg ctgctaaagt tgttgacacc 1560

gaccagcgtg ttacccgtgg tcagtacaaa gacgttgcta acaaatggca cgaactgaac 1620

tctttcaccg ttgctccgat caaaggtacc 1650

<210> 27

<211> 746

<212> PRT

<213> Acinetobacter baumannii

<220>

<221> PEPTIDE

<222> (1)..(746)

<223> Amino acid sequence of putative ferric siderophore receptor

protein

<400> 27

Met Thr Pro Cys Cys Leu Ala Ile Ser Ala Ile Phe Ala Gln Gln Ala

1 5 10 15

Tyr Ala Glu Thr Val Thr Gln Thr Ala Glu Val Ser Glu Asn Ala Thr

20 25 30

Gln Lys Pro Val Ala Gln Leu Gln Lys Ile Val Val Thr Ala Thr Arg

35 40 45

Thr Pro Lys Asn Ile Ala Glu Ile Ala Gly Thr Val Gln Ser Ile Asp

50 55 60

Gln Lys Gln Ile Ile Gln Gln Ala Thr Ala Gly Arg Lys Val Ala Asp

65 70 75 80

Ile Leu Ala Gln Leu Val Pro Ser Leu Ala Ser Ser Ser Gly Thr Thr

85 90 95

Ser Asn Tyr Gly Gln Thr Met Arg Gly Arg Asn Val Leu Val Met Ile

100 105 110

Asp Gly Val Ser Gln Thr Gly Ser Arg Asp Val Ser Arg Gln Leu Asn

115 120 125

Ser Ile Ser Pro Gly Met Ile Glu Arg Ile Glu Val Ile Ser Gly Ala

130 135 140

Thr Ser Ile Tyr Gly Ser Gly Ala Thr Gly Gly Ile Ile Asn Ile Ile

145 150 155 160

Thr Lys Arg Ala Asp Thr Ser Lys Pro Leu Ser Phe Glu Thr Lys Val

165 170 175

Gly Ile Thr Ser Ser Asp Thr Phe Arg Ser Asp Gly Leu Ala Tyr Glu

180 185 190

Val Gly Gln Ser Val Ser Phe Asn Lys Gly Asn Ile Asp Gly Phe Leu

195 200 205

Gly Ala Asn Phe Thr Ser Arg Gly Ser Gln Phe Asp Gly Asn Gly Asp

210 215 220

Arg Ile Ser Leu Ser Pro Trp Gln Gly Ser Thr Met Asp Thr Asp Thr

225 230 235 240

Ile Asp Val Asn Gly Arg Leu Asn Phe Asn Leu Asn Asp Thr Gln Thr

245 250 255

Leu Ser Phe Gly Ala Gln Tyr Tyr Lys Asp Lys Gln Asp Thr Asp Tyr

260 265 270

Gly Pro Asp Tyr Ser Tyr Leu Pro Thr Thr Ser Lys Ser Asn Asp Ala

275 280 285

Thr Thr Pro Thr Tyr Lys Ala Ile Lys Gly Leu Lys Leu Ser Asn Pro

290 295 300

Leu Phe Thr Glu Arg Tyr Ala Val Asn Ser Gln Tyr Gln Asn Gln Asp

305 310 315 320

Phe Leu Gly Gln Ile Leu Asn Val Glu Ala Tyr Tyr Arg Asn Glu Lys

325 330 335

Ser Arg Phe Phe Pro Tyr Gly Leu Ser Asn Lys Ser Val Thr Ser Val

340 345 350

Asn Gln Ser Gln Ser Glu Ile Glu Val Ala Gly Leu Arg Ser Thr Met

355 360 365

Gln Thr Asp Leu Asn Ile Ala Asn Arg Asp Met Lys Ile Thr Tyr Gly

370 375 380

Leu Asp Tyr Asp Trp Glu Lys Asp Lys Gln Phe Val Asp Ile Leu Ala

385 390 395 400

Thr Gln Tyr Pro Tyr Leu Val Tyr Thr Pro Thr Gly Gln Arg Lys Gly

405 410 415

Tyr Gly Pro Asn Thr Glu Ile Gln Asn Ile Gly Ala Phe Val Gln Ser

420 425 430

Asp Tyr Ala Val Thr Asp Lys Leu Asn Leu Gln Ala Gly Ile Arg Tyr

435 440 445

Gln Tyr Ile Gln Ala Asp Thr Asp Ala Tyr Ile Pro Ser Arg Glu Thr

450 455 460

Thr Met Val Pro Ala Gly Ser Thr His Asp Asp Lys Pro Leu Phe Asn

465 470 475 480

Leu Gly Ala Val Tyr Lys Leu Thr Asp Ala Gln Gln Val Tyr Ala Asn

485 490 495

Phe Ser Gln Gly Phe Ser Phe Pro Asp Val Gln Arg Met Leu Arg Asp

500 505 510

Val Ser Thr Tyr Thr Val Ser Thr Ala Asn Leu Gln Pro Ile Thr Val

515 520 525

Asn Ser Tyr Glu Leu Gly Trp Arg Leu Asn Gln Asp Asp Gly Leu Asn

530 535 540

Leu Gly Leu Thr Gly Phe Tyr Asn Thr Ser Asp Lys Thr Val Gln Phe

545 550 555 560

Asn Asn Arg Ala Ala Lys Val Val Asp Thr Asp Gln Arg Val Tyr Gly

565 570 575

Ala Glu Ala Thr Ile Ser Tyr Pro Phe Met Glu Asn Tyr Lys Val Gly

580 585 590

Gly Thr Leu Gly Tyr Thr Arg Gly Gln Tyr Lys Asp Val Ala Asn Lys

595 600 605

Trp His Glu Leu Asn Ser Phe Thr Val Ala Pro Val Lys Gly Thr Leu

610 615 620

Phe Ala Glu Trp Asp Asn Asn Glu Gly Tyr Gly Val Arg Val Gln Met

625 630 635 640

Gln Ala Ile Lys Gly Thr Asn Lys Ala Tyr Lys Asp Asp Arg Glu Leu

645 650 655

Ala Ala Phe Ala Thr Thr Gln Asp Glu Ala Phe Gln Asn Ala Val Lys

660 665 670

Asn Asp Ala Asn Ser Ala Ala Gln Ile Lys Gly Tyr Thr Thr Met Asp

675 680 685

Val Leu Ala His Phe Pro Ala Trp Lys Gly Arg Val Asp Phe Gly Val

690 695 700

Tyr Asn Val Trp Asn Arg Gln Tyr Arg Thr Val Phe Ala Gln Gln Ala

705 710 715 720

Ala Val Ser Asn Ala Asn Pro Leu Leu Ala Ile Pro Ala Glu Gly Arg

725 730 735

Thr Tyr Gly Leu Ser Tyr Thr Phe Asn Tyr

740 745

<210> 28

<211> 679

<212> PRT

<213> Acinetobacter baumannii

<220>

<221> PEPTIDE

<222> (1)..(679)

<223> Amino acid sequence of putative ferric hydroxamate siderophore

receptor

<400> 28

Met Gln Thr Ile Gln Val Lys Ala Ser Asp Ala Glu Gln Ser Ser Glu

1 5 10 15

Gln Thr Lys Ala Tyr Asn Val Lys Asn Ser Ser Ser Ala Thr Lys Leu

20 25 30

Asn Ile Glu Ala Lys Glu Thr Pro Gln Thr Ile Asn Val Val Thr Arg

35 40 45

Gln Gln Ile Glu Asp Phe Gly Leu Thr Ser Thr Arg Asp Val Leu Arg

50 55 60

Asn Thr Pro Gly Val Thr Val Ser Asn Gln Glu Thr Glu Arg Thr Thr

65 70 75 80

Tyr Met Ala Arg Gly Phe Glu Ile Ser Asn Ile Leu Thr Asp Gly Val

85 90 95

Gly Phe Pro Leu Ser Gly Tyr Asn Tyr Asn Asn Thr Asn Pro Asp Thr

100 105 110

Tyr Phe Tyr Asp Arg Val Glu Val Val Lys Gly Ala Asp Ser Leu Thr

115 120 125

Asn Ala Phe Gly Asp Pro Ser Ala Thr Ile Asn Asn Ile Arg Lys Arg

130 135 140

Pro Thr Gln Glu Phe Gln Ala Ser Asp Gly Val Ser Tyr Gly Ser Trp

145 150 155 160

Asp Thr Gln Arg Tyr Glu Ala Asp Val Ser Gly Ser Ile Leu Pro Ser

165 170 175

Gly Lys Val Arg Gly Arg Ile Met Gly Tyr Glu Gln Thr Gly Asp Ser

180 185 190

Tyr Leu Asp Arg Tyr Ser Ala Glu Lys Asn Gly Phe Ala Gly Ile Val

195 200 205

Glu Ala Asp Leu Thr Asp Ser Thr Leu Leu Thr Ala Gly Tyr Ser Gln

210 215 220

Glu Gln Asn Lys Pro Asn Ala Asn Asn Trp Gly Ala Leu Pro Leu Leu

225 230 235 240

Asp Ala Asn Gly Lys Gln Ile Ser Tyr Asp Arg Ser Tyr Asn Pro Asn

245 250 255

Pro Asp Trp Ala His Trp Asp Asn Glu Thr Gln Asn Ala Phe Val Glu

260 265 270

Leu Lys Gln Lys Leu Asn Asp Gln Trp Asn Ala Lys Leu Thr Tyr Asn

275 280 285

Tyr Leu Asp Thr Lys His Asn Ser Arg Leu Leu Tyr Tyr Tyr Gly Tyr

290 295 300

Pro Lys Ala Asp Gly Ser Gly Val Ser Leu Thr Pro Trp Gly Gly Gln

305 310 315 320

Glu His Gln Glu Lys His Ala Val Asp Phe Asn Leu Glu Gly Thr Tyr

325 330 335

Lys Leu Phe Asn Arg Glu His Glu Ala Thr Leu Gly Tyr Ser Tyr Val

340 345 350

Arg Asn His Gln Gln Asp Lys Gln Ser Thr Gly Thr Ile Asn Asp Ser

355 360 365

Asn Val Ile Lys Ser Thr Thr Thr Asp Trp Ala Ser Trp Thr Pro Gln

370 375 380

Ser Ile Thr Trp Ser Ala Phe Thr Glu Ala Ala Asn Tyr Lys Gln Asn

385 390 395 400

Ile Asn Ser Ile Tyr Ala Ala Thr Arg Leu His Leu Asn Glu Asp Leu

405 410 415

Lys Leu Leu Leu Gly Val Asn Tyr Val Gln Ala Glu Ser Lys Gly Glu

420 425 430

Ser Tyr Ser Ser Pro Met Ser Tyr Ser Glu Ser Lys Val Ser Pro Tyr

435 440 445

Val Gly Leu Thr Tyr Asn Phe Thr Pro Glu Tyr Thr Gly Tyr Met Ser

450 455 460

Tyr Thr Ser Ile Phe Arg Pro Gln Thr Gly Ile Asp Lys Asp Thr Asn

465 470 475 480

Gln Ala Leu Lys Pro Ile Glu Gly Lys Ser Tyr Glu Met Gly Val Lys

485 490 495

Ser Ser Trp Leu Asp Asp Arg Leu Thr Gly Thr Leu Ser Val Phe Lys

500 505 510

Thr Glu Gln Asn Asn Tyr Pro Leu Arg Asn Ser Asp Gly Asn Pro Leu

515 520 525

Asn Arg Lys Val Pro Thr Ser Asp Leu Glu Ser Gln Gly Val Glu Val

530 535 540

Gly Leu Ser Gly Gln Ile Thr Asp Asn Val Asn Leu Ser Leu Gly Tyr

545 550 555 560

Ala Gln Phe Ser Ile Lys Asp Thr Lys Asn Gly Gly Glu Ala Arg Ile

565 570 575

Tyr Asn Pro Asn Gln Thr Leu Asn Leu Leu Thr Thr Tyr Thr Pro Pro

580 585 590

Val Leu Pro Lys Leu Lys Val Gly Ala Gly Leu Gln Trp Gln Asp Gly

595 600 605

Ile Lys Leu Tyr Asp Ser Asn Val Asn Gly Thr Ile Lys Gln Asp Ala

610 615 620

Tyr Ala Leu Val Asn Leu Met Ala Ser Tyr Glu Val Asn Asp His Ile

625 630 635 640

Thr Leu Gln Ala Asn Gly Asn Asn Ile Phe Asp Lys Lys Tyr Leu Asn

645 650 655

Ser Phe Pro Asp Gly Gln Ala Phe Tyr Gly Ala Pro Ala Asn Tyr Thr

660 665 670

Val Ala Val Lys Phe Lys Tyr

675

<210> 29

<211> 829

<212> PRT

<213> Acinetobacter baumannii

<400> 29

Met Pro Leu Ala Leu Val Ser Ala Met Ala Ala Val Gln Gln Ala Tyr

1 5 10 15

Ala Ala Asp Asp Phe Val Val Arg Asp Ile Arg Val Asn Gly Leu Val

20 25 30

Arg Leu Thr Pro Ala Asn Val Tyr Thr Met Leu Pro Ile Asn Ser Gly

35 40 45

Asp Arg Val Asn Glu Pro Met Ile Ala Glu Ala Ile Arg Thr Leu Tyr

50 55 60

Ala Thr Gly Leu Phe Asp Asp Ile Lys Ala Ser Lys Glu Asn Asp Thr

65 70 75 80

Leu Val Phe Asn Val Ile Glu Arg Pro Ile Ile Ser Lys Leu Glu Phe

85 90 95

Lys Gly Asn Lys Leu Ile Pro Lys Glu Ala Leu Glu Gln Gly Leu Lys

100 105 110

Lys Met Gly Ile Ala Glu Gly Glu Val Phe Lys Lys Ser Ala Leu Gln

115 120 125

Thr Ile Glu Thr Glu Leu Glu Gln Gln Tyr Thr Gln Gln Gly Arg Tyr

130 135 140

Asp Ala Asp Val Thr Val Asp Thr Val Ala Arg Pro Asn Asn Arg Val

145 150 155 160

Glu Leu Lys Ile Asn Phe Asn Glu Gly Thr Pro Ala Lys Val Phe Asp

165 170 175

Ile Asn Val Ile Gly Asn Thr Val Phe Lys Asp Ser Glu Ile Lys Gln

180 185 190

Ala Phe Ala Val Lys Glu Ser Gly Trp Ala Ser Val Val Thr Arg Asn

195 200 205

Asp Arg Tyr Ala Arg Glu Lys Met Ala Ala Ser Leu Glu Ala Leu Arg

210 215 220

Ala Met Tyr Leu Asn Lys Gly Tyr Ile Asn Phe Asn Ile Asn Asn Ser

225 230 235 240

Gln Leu Asn Ile Ser Glu Asp Lys Lys His Ile Phe Ile Glu Val Ala

245 250 255

Val Asp Glu Gly Ser Gln Phe Lys Phe Gly Gln Thr Lys Phe Leu Gly

260 265 270

Asp Ala Leu Tyr Lys Pro Glu Glu Leu Gln Ala Leu Lys Ile Tyr Lys

275 280 285

Asp Gly Asp Thr Tyr Ser Gln Glu Lys Val Asn Ala Val Lys Gln Leu

290 295 300

Leu Leu Arg Lys Tyr Gly Asn Ala Gly Tyr Tyr Phe Ala Asp Val Asn

305 310 315 320

Ile Val Pro Gln Ile Asn Asn Glu Thr Gly Val Val Asp Leu Asn Tyr

325 330 335

Tyr Val Asn Pro Gly Gln Gln Val Thr Val Arg Arg Ile Asn Phe Thr

340 345 350

Gly Asn Ser Lys Thr Ser Asp Glu Val Leu Arg Arg Glu Met Arg Gln

355 360 365

Met Glu Gly Ala Leu Ala Ser Asn Glu Lys Ile Asp Leu Ser Lys Val

370 375 380

Arg Leu Glu Arg Thr Gly Phe Phe Lys Thr Val Asp Ile Lys Pro Ala

385 390 395 400

Arg Ile Pro Asn Ser Pro Asp Gln Val Asp Leu Asn Val Asn Val Glu

405 410 415

Glu Gln His Ser Gly Thr Thr Thr Leu Ala Val Gly Tyr Ser Gln Ser

420 425 430

Gly Gly Ile Thr Phe Gln Ala Gly Leu Ser Gln Thr Asn Phe Met Gly

435 440 445

Thr Gly Asn Arg Val Ala Ile Asp Leu Ser Arg Ser Glu Thr Gln Asp

450 455 460

Tyr Tyr Asn Leu Ser Val Thr Asp Pro Tyr Phe Thr Ile Asp Gly Val

465 470 475 480

Ser Arg Gly Tyr Asn Val Tyr Tyr Arg Lys Thr Lys Leu Asn Asp Asp

485 490 495

Tyr Asn Val Asn Asn Tyr Val Thr Asp Ser Phe Gly Gly Ser Leu Ser

500 505 510

Phe Gly Tyr Pro Ile Asp Glu Asn Gln Ser Leu Ser Ala Ser Val Gly

515 520 525

Val Asp Asn Thr Lys Val Thr Thr Gly Ala Phe Val Ser Thr Tyr Val

530 535 540

Arg Asp Tyr Leu Leu Ala Asn Gly Gly Lys Thr Thr Ser Thr Asn Thr

545 550 555 560

Tyr Cys Leu Val Asp Leu Val Gln Asp Pro Gln Thr Gly Leu Tyr Lys

565 570 575

Cys Pro Glu Gly Gln Thr Ser Gln Pro Tyr Gly Asn Ala Phe Glu Gly

580 585 590

Glu Phe Phe Thr Tyr Asn Leu Asn Leu Gly Trp Ser Tyr Asn Thr Leu

595 600 605

Asn Arg Pro Ile Phe Pro Thr Ser Gly Met Ser His Arg Val Gly Leu

610 615 620

Glu Ile Gly Leu Pro Gly Ser Asp Val Asp Tyr Gln Lys Val Thr Tyr

625 630 635 640

Asp Thr Gln Ala Phe Phe Pro Ile Gly Ser Thr Gly Phe Val Leu Arg

645 650 655

Gly Tyr Gly Lys Leu Gly Tyr Gly Asn Asp Leu Pro Phe Tyr Lys Asn

660 665 670

Phe Tyr Ala Gly Gly Tyr Gly Ser Val Arg Gly Tyr Asp Asn Ser Thr

675 680 685

Leu Gly Pro Lys Tyr Ala Ser Val Asn Leu Gln Glu Glu Lys Lys Asn

690 695 700

Asp Ser Ser Pro Glu Glu Val Gly Gly Asn Ala Leu Val Gln Phe Gly

705 710 715 720

Thr Glu Leu Val Leu Pro Met Pro Phe Lys Gly Asp Trp Thr Arg Gln

725 730 735

Val Arg Pro Val Leu Phe Ala Glu Gly Gly Gln Val Phe Asp Thr Lys

740 745 750

Cys Asp Val Arg Ser Tyr Ser Met Ile Met Asn Gly Gln Gln Ile Ser

755 760 765

Asp Ala Lys Lys Tyr Cys Glu Asp Asn Tyr Gly Phe Asp Leu Gly Asn

770 775 780

Leu Arg Tyr Ser Val Gly Val Gly Val Thr Trp Ile Thr Met Ile Gly

785 790 795 800

Pro Leu Ser Leu Ser Tyr Ala Phe Pro Leu Asn Asp Lys Pro Gly Asp

805 810 815

Glu Thr Lys Glu Ile Gln Phe Glu Ile Gly Arg Thr Phe

820 825

<210> 30

<211> 458

<212> PRT

<213> Acinetobacter baumannii

<400> 30

Met Arg Gly Pro Glu Pro Val Val Lys Thr Asp Ile Pro Gln Ser Tyr

1 5 10 15

Ala Tyr Asn Ser Ala Ser Gly Thr Ser Ile Ala Glu Gln Gly Tyr Lys

20 25 30

Gln Phe Phe Ala Asp Pro Arg Leu Leu Glu Val Ile Asp Leu Ala Leu

35 40 45

Ala Asn Asn Arg Asp Leu Arg Thr Ala Thr Leu Asn Ile Glu Arg Ala

50 55 60

Gln Gln Gln Tyr Gln Ile Thr Gln Asn Asn Gln Leu Pro Thr Ile Gly

65 70 75 80

Ala Ser Gly Ser Ala Ile Arg Gln Val Ser Gln Ser Arg Asp Pro Asn

85 90 95

Asn Pro Tyr Ser Thr Tyr Gln Val Gly Leu Gly Val Thr Ala Tyr Glu

100 105 110

Leu Asp Phe Trp Gly Arg Val Arg Ser Leu Lys Asp Ala Ala Leu Asp

115 120 125

Ser Tyr Leu Ala Thr Gln Ser Ala Arg Asp Ser Thr Gln Ile Ser Leu

130 135 140

Ile Ser Gln Val Ala Gln Ala Trp Leu Asn Tyr Ser Phe Ala Thr Ala

145 150 155 160

Asn Leu Arg Leu Ala Glu Gln Thr Leu Lys Ala Gln Leu Asp Ser Tyr

165 170 175

Asn Leu Asn Lys Lys Arg Phe Asp Val Gly Ile Asp Ser Glu Val Pro

180 185 190

Leu Arg Gln Ala Gln Ile Ser Val Glu Thr Ala Arg Asn Asp Val Ala

195 200 205

Asn Tyr Lys Thr Gln Ile Ala Gln Ala Gln Asn Leu Leu Asn Leu Leu

210 215 220

Val Gly Gln Pro Val Pro Gln Asn Leu Leu Pro Thr Gln Pro Val Lys

225 230 235 240

Arg Ile Ala Gln Gln Asn Val Phe Thr Ala Gly Leu Pro Ser Asp Leu

245 250 255

Leu Asn Asn Arg Pro Asp Val Lys Ala Ala Glu Tyr Asn Leu Ser Ala

260 265 270

Ala Gly Ala Asn Ile Gly Ala Ala Lys Ala Arg Leu Phe Pro Thr Ile

275 280 285

Ser Leu Thr Gly Ser Ala Gly Tyr Ala Ser Thr Asp Leu Ser Asp Leu

290 295 300

Phe Lys Ser Gly Gly Phe Val Trp Ser Val Gly Pro Ser Leu Asp Leu

305 310 315 320

Pro Ile Phe Asp Trp Gly Thr Arg Arg Ala Asn Val Lys Ile Ser Glu

325 330 335

Thr Asp Gln Lys Ile Ala Leu Ser Asp Tyr Glu Lys Ser Val Gln Ser

340 345 350

Ala Phe Arg Glu Val Asn Asp Ala Leu Ala Thr Arg Ala Asn Ile Gly

355 360 365

Glu Arg Leu Thr Ala Gln Gln Arg Leu Val Glu Ala Thr Asn Arg Asn

370 375 380

Tyr Thr Leu Ser Asn Ala Arg Phe Arg Ala Gly Ile Asp Ser Tyr Leu

385 390 395 400

Thr Val Leu Asp Ala Gln Arg Ser Ser Tyr Ala Ala Glu Gln Gly Leu

405 410 415

Leu Leu Leu Gln Gln Ala Asn Leu Asn Asn Gln Ile Glu Leu Tyr Lys

420 425 430

Thr Leu Gly Gly Gly Leu Lys Ala Asn Thr Ser Asp Thr Val Val His

435 440 445

Gln Pro Ser Ser Ala Glu Leu Lys Lys Gln

450 455

<210> 31

<211> 412

<212> PRT

<213> Acinetobacter baumannii

<400> 31

Met Met Ser Gly Ala Asn Ala Ala Thr Ser Asp Lys Glu Glu Ile Arg

1 5 10 15

Lys Leu Arg Gln Glu Val Glu Ala Leu Lys Ala Leu Val Gln Glu Gln

20 25 30

Arg Gln Val Gln Gln Gln Gln Gln Gln Val Gln Gln Gln Gln Gln Val

35 40 45

Gln Leu Ala Glu Val Lys Ala Gln Pro Gln Pro Val Ala Ala Pro Val

50 55 60

Ser Pro Leu Ala Gly Phe Lys Ser Lys Ala Gly Ala Asp Val Asn Leu

65 70 75 80

Tyr Gly Phe Val Arg Gly Asp Ala Asn Tyr Ile Ile Glu Gly Ala Asp

85 90 95

Asn Asp Phe Gly Asp Val Ser Lys Ser Asp Gly Lys Thr His Asp Lys

100 105 110

Leu Arg Ala Thr Ala Lys Thr Thr Arg Leu Gly Leu Asp Phe Asn Thr

115 120 125

Pro Val Gly Asp Asp Lys Val Gly Gly Lys Ile Glu Val Asp Phe Ala

130 135 140

Gly Ser Thr Thr Asp Ser Asn Gly Ser Leu Arg Ile Arg His Ala Tyr

145 150 155 160

Leu Thr Tyr Asn Asn Trp Leu Phe Gly Gln Thr Thr Ser Asn Phe Leu

165 170 175

Ser Asn His Ala Pro Glu Met Ile Asp Phe Ser Thr Asn Ile Gly Gly

180 185 190

Gly Thr Lys Arg Val Pro Gln Val Arg Tyr Asn Tyr Lys Leu Gly Pro

195 200 205

Thr Thr Gln Leu Phe Val Ser Ala Glu Lys Gly Asp Ser Thr Thr Ser

210 215 220

Val Thr Gly Asp Ser Ile Lys Tyr Ser Leu Pro Ala Leu Thr Ala Lys

225 230 235 240

Ile Thr Gln Gly Tyr Ala Glu Gly Arg Gly Ser Ala Ser Ala Arg Val

245 250 255

Leu Val Glu Asn Tyr Lys Ser Gln Leu Ala Asp Asp Asp Lys Thr Gly

260 265 270

Trp Gly Val Ala Val Gly Thr Asp Phe Lys Val Ser Asp Pro Leu Lys

275 280 285

Leu Phe Ala Asp Ala Ser Tyr Val Val Gly Asp Asn Ser Tyr Leu Tyr

290 295 300

Gly Ser Asn Ser Pro Tyr Ala Val Asp Gly Asn Ser Ile Glu Gln Asn

305 310 315 320

Glu Phe Val Ala Val Gln Val Gly Gly Thr Tyr Lys Ile Leu Pro Asn

325 330 335

Leu Arg Ser Thr Leu Ala Tyr Gly Ala Gln Phe Ser Asp Asp Gly Thr

340 345 350

Asp Tyr Ala Arg Leu Asn Ala Ser Ala Asn Glu Lys Val Gln Gln Ala

355 360 365

Trp Ile Asn Phe Ile Tyr Thr Pro Val Lys Pro Ile Asp Leu Gly Val

370 375 380

Glu Tyr Val Asn Gly Lys Arg Asp Thr Phe Asp Gly Lys Ser Tyr Lys

385 390 395 400

Asp Asn Arg Val Gly Leu Met Ala Lys Tyr Ser Phe

405 410

<210> 32

<211> 233

<212> PRT

<213> putative outer membrane protein

<400> 32

Tyr Gln Ala Glu Val Gly Gly Ser Tyr Asn Tyr Leu Asp Pro Asp Asn

1 5 10 15

Gly Ser Ser Val Ser Lys Phe Gly Val Asp Gly Thr Tyr Tyr Phe Asn

20 25 30

Pro Val Gln Thr Arg Asn Ala Pro Leu Ala Glu Ala Ala Phe Leu Asn

35 40 45

Arg Ala Ser Asn Val Asn Ala His Val Asn Tyr Gly Asp Asn Ser Gly

50 55 60

Thr Lys Asp Thr Gln Tyr Gly Val Gly Val Glu Tyr Phe Val Pro Asn

65 70 75 80

Ser Asp Phe Tyr Leu Ser Gly Asp Val Gly Arg Asn Glu Arg Glu Ile

85 90 95

Asp Asn Thr Asn Ile Asp Ser Lys Val Thr Thr Tyr Ala Ala Glu Val

100 105 110

Gly Tyr Leu Pro Ala Pro Gly Leu Leu Leu Ala Leu Gly Val Lys Gly

115 120 125

Tyr Asp Glu Lys Asp Gly Lys Asp Gly Ala Asp Pro Thr Val Arg Ala

130 135 140

Lys Tyr Val Thr Gln Val Gly Gln His Asp Val Asn Leu Glu Ala Tyr

145 150 155 160

Gly Ala Phe Gly Asp Leu Asp Glu Tyr Lys Val Arg Gly Asp Tyr Tyr

165 170 175

Ile Asp Lys Thr Leu Ser Leu Gly Val Asp Tyr Tyr Asn Asn Asp Leu

180 185 190

Thr Asp Lys Asp Glu Phe Gly Ile Asn Ala Lys Lys Phe Leu Asn Gln

195 200 205

Gln Val Ser Val Glu Gly Arg Val Gly Phe Gly Asp Asn Asp Asn Thr

210 215 220

Tyr Gly Val Arg Ala Ala Tyr Arg Phe

225 230

<210> 33

<211> 364

<212> PRT

<213> Acinetobacter baumannii

<400> 33

Met Gly Cys Ser Ser Asn Pro Ser Lys Lys Glu Val Val Asp Thr Gly

1 5 10 15

Pro Gln Ser Ser Glu Gln Ala Tyr Phe Asp Lys Ala Gln Lys Ala Leu

20 25 30

Asp Arg Gly Gln Tyr Leu Asp Ala Thr Lys Ser Leu Glu Ala Ile Asp

35 40 45

Thr Tyr Tyr Pro Thr Gly Gln Tyr Ala Gln Gln Ala Gln Leu Glu Leu

50 55 60

Leu Tyr Ser Lys Phe Lys Gln Lys Asp Tyr Glu Gly Ala Ile Ala Leu

65 70 75 80

Ala Glu Arg Phe Ile Arg Leu Asn Pro Gln His Pro Asn Val Asp Tyr

85 90 95

Ala Tyr Tyr Val Arg Gly Val Ser Asn Met Glu Met Asn Tyr Asp Ser

100 105 110

Leu Leu Arg Tyr Thr Ser Leu Gln Gln Ser His Arg Asp Val Ser Tyr

115 120 125

Leu Lys Val Ala Tyr Gln Asn Phe Val Asp Leu Ile Arg Arg Phe Pro

130 135 140

Ser Ser Gln Tyr Ser Val Asp Ala Ala Gln Arg Met Lys Phe Ile Gly

145 150 155 160

Gln Glu Leu Ala Glu Ser Glu Met Asn Ala Ala Arg Phe Asn Val Lys

165 170 175

Arg Lys Ala Trp Ile Ala Ala Ala Glu Arg Ser Gln Trp Val Ile Glu

180 185 190

His Tyr Pro Gln Thr Pro Gln Val Pro Glu Ala Leu Ala Thr Leu Ala

195 200 205

Tyr Ser Tyr Asp Gln Leu Gly Asp Lys Ala Thr Ser Gln Gln Tyr Ile

210 215 220

Glu Val Leu Lys Leu Asn Tyr Pro Ser Leu Val Asn Lys Asn Gly Thr

225 230 235 240

Val Asn Met Arg Ala Ala Arg Lys Glu Gly Asn Trp Ile Asn Arg Ala

245 250 255

Thr Leu Gly Ile Leu Gly Arg Glu Ser Lys Ser Val Thr Pro Asp Thr

260 265 270

Thr Thr Ser Ser Glu Ala Glu Pro Lys Arg Ser Leu Leu Asn Arg Val

275 280 285

Ser Phe Gly Leu Ile Gly Asn Ser Gly Lys Glu Glu Thr Glu Glu Thr

290 295 300

Thr Asn Thr Pro Val Glu Ala Pro Lys Ser Glu Arg Ser Trp Thr Asn

305 310 315 320

Arg Leu Ser Phe Gly Leu Leu Asp Lys Pro Lys Pro Lys Ala Ala Glu

325 330 335

Gly Ala Thr Ile Ala Pro Ala Thr Ser Ser Ser Glu Ala Pro Ser Ala

340 345 350

Ser Pro Ala Asp Asn Glu Ala Asp Asp Ala Ala Gln

355 360

<210> 34

<211> 356

<212> PRT

<213> Acinetobacter baumannii

<400> 34

Met Lys Leu Ser Arg Ile Ala Leu Ala Thr Met Leu Val Ala Ala Pro

1 5 10 15

Leu Ala Ala Ala Asn Ala Gly Val Thr Val Thr Pro Leu Leu Leu Gly

20 25 30

Tyr Thr Phe Gln Asp Ser Gln His Asn Asn Gly Gly Lys Asp Gly Asn

35 40 45

Leu Thr Asn Gly Pro Glu Leu Gln Asp Asp Leu Phe Val Gly Ala Ala

50 55 60

Leu Gly Ile Glu Leu Thr Pro Trp Leu Gly Phe Glu Ala Glu Tyr Asn

65 70 75 80

Gln Val Lys Gly Asp Val Asp Gly Ala Ser Ala Gly Ala Glu Tyr Lys

85 90 95

Gln Lys Gln Ile Asn Gly Asn Phe Tyr Val Thr Ser Asp Leu Ile Thr

100 105 110

Lys Asn Tyr Asp Ser Lys Ile Lys Pro Tyr Val Leu Leu Gly Ala Gly

115 120 125

His Tyr Lys Tyr Asp Phe Asp Gly Val Asn Arg Gly Thr Arg Gly Thr

130 135 140

Ser Glu Glu Gly Thr Leu Gly Asn Ala Gly Val Gly Ala Phe Trp Arg

145 150 155 160

Leu Asn Asp Ala Leu Ser Leu Arg Thr Glu Ala Arg Ala Thr Tyr Asn

165 170 175

Ala Asp Glu Glu Phe Trp Asn Tyr Thr Ala Leu Ala Gly Leu Asn Val

180 185 190

Val Leu Gly Gly His Leu Lys Pro Ala Ala Pro Val Val Glu Val Ala

195 200 205

Pro Val Glu Pro Thr Pro Val Ala Pro Gln Pro Gln Glu Leu Thr Glu

210 215 220

Asp Leu Asn Met Glu Leu Arg Val Phe Phe Asp Thr Asn Lys Ser Asn

225 230 235 240

Ile Lys Asp Gln Tyr Lys Pro Glu Ile Ala Lys Val Ala Glu Lys Leu

245 250 255

Ser Glu Tyr Pro Asn Ala Thr Ala Arg Ile Glu Gly His Thr Asp Asn

260 265 270

Thr Gly Pro Arg Lys Leu Asn Glu Arg Leu Ser Leu Ala Arg Ala Asn

275 280 285

Ser Val Lys Ser Ala Leu Val Asn Glu Tyr Asn Val Asp Ala Ser Arg

290 295 300

Leu Ser Thr Gln Gly Phe Ala Trp Asp Gln Pro Ile Ala Asp Asn Lys

305 310 315 320

Thr Lys Glu Gly Arg Ala Met Asn Arg Arg Val Phe Ala Thr Ile Thr

325 330 335

Gly Ser Arg Thr Val Val Val Gln Pro Gly Gln Glu Ala Ala Ala Pro

340 345 350

Ala Ala Ala Gln

355

<210> 35

<211> 581

<212> PRT

<213> Acinetobacter baumannii

<400> 35

Met Asn Gln Glu Thr Gly Arg Gly Val Thr Arg Gly Thr Lys Leu Tyr

1 5 10 15

Val Lys Asp Val Pro Val Leu Ala Val Pro Tyr Phe Asn Phe Pro Ile

20 25 30

Asp Asp Arg Arg Thr Thr Gly Ile Leu Asn Pro Gln Phe Gly Phe Ser

35 40 45

Asn Asp Gly Gly Ile Glu Leu Ser Val Pro Val Tyr Leu Asn Leu Ala

50 55 60

Pro Asn Tyr Asp Ala Thr Ile Thr Pro Arg Tyr Leu Ala Asp Arg Gly

65 70 75 80

Ala Met Leu Gln Gly Glu Phe Arg Tyr Leu Thr Asp Gly Phe Gly Ala

85 90 95

Gly Gln Ile Trp Gly Gly Ile Leu Pro Ser Asp Lys Glu Tyr Asp Asp

100 105 110

Lys Asp Arg Lys Asp Phe His Phe Leu His Asn Trp Asp Ile Asn Asp

115 120 125

Gln Trp Ser Thr Asn Leu Glu Tyr Asn Tyr Ala Ser Asp Lys Asp Tyr

130 135 140

Phe Ser Asp Leu Asp Ser Ser Pro Ile Ser Lys Thr Asp Leu Asn Leu

145 150 155 160

Arg Arg Ala Trp Glu Leu Asn Tyr Gln His Gly Ile Pro Gly Leu Lys

165 170 175

Ala Gln Leu Lys Val Glu Asp Phe Gln Thr Leu Asp Pro Gln Val Lys

180 185 190

Asp Ala Asp Lys Pro Tyr Ala Arg Leu Pro Gln Phe Leu Leu Asn Tyr

195 200 205

Val Thr Gly Asn Pro Leu Gly Leu Gln Tyr Glu Phe Asn Asn Asp Thr

210 215 220

Ala Tyr Phe Lys Lys Ser Ile Asn Asp Asn Ser Ala Gln Glu Ser Ser

225 230 235 240

Gly Thr Arg Ile Tyr Asn Gln Phe Ala Thr Arg Tyr Asn Tyr Arg Thr

245 250 255

Pro Ala Ala Phe Val Ile Pro Glu Val Ser Val Arg Ser Ile Gln Thr

260 265 270

Phe Tyr Asp Lys Asp Thr Gln Leu Asn Asn Pro Gly Gly Ser Glu Asn

275 280 285

Lys Ser Val Val Val Pro Gln Phe Thr Leu Asp Thr Gly Leu Asn Phe

290 295 300

Glu Arg Glu Gly Lys Tyr Leu Gln Thr Leu Thr Pro Arg Ala Phe Tyr

305 310 315 320

Ala Tyr Ala Pro Tyr Lys Asn Gln Asp Gly Tyr Pro Asn Phe Asp Ser

325 330 335

Thr Thr Ala Ser Ile Ser Tyr Asp Gln Leu Phe Asn Pro Tyr Arg Phe

340 345 350

Tyr Gly His Asp Arg Leu Glu Asp Asn Asn Phe Leu Ser Leu Gly Val

355 360 365

Ser Tyr Ser Leu Phe Asp Thr Val Gly Leu Glu Arg Leu Arg Ala Ser

370 375 380

Val Gly Gln Ser Tyr Tyr Phe Glu Asp Arg Arg Val Thr Leu Lys Gln

385 390 395 400

Gly Gln Asp Glu Phe Asp Thr Glu Arg Lys Thr Gly Pro Val Ile Ser

405 410 415

Leu Ser Ser Gln Leu Asn Gln Asn Phe Thr Ile Ala Ala Asn Ser Ala

420 425 430

Trp Met Ser Asn Gly Asp Asn Ala Gln Arg Asp Phe Gln Val Tyr Tyr

435 440 445

Thr Gly Asp Lys Gly Asn Leu Tyr Asn Leu Gly Tyr Phe Tyr Arg Lys

450 455 460

Asp Ile Pro Gly Arg Gln Asp Thr Tyr Asp Gln Val Val Ala Ser Phe

465 470 475 480

Ile Gln Pro Ile Lys Asp Asn Trp Arg Ile Met Gly His Val Gln Tyr

485 490 495

Asp Met Asp Asn Asp Val Ala Arg Glu Leu Leu Leu Gly Val Asn Tyr

500 505 510

Glu Ser Cys Cys Trp Gly Ile Ser Val Tyr Gly Arg Ser Tyr Tyr Asn

515 520 525

Asp Leu Asp Asp Pro Lys Thr Ser Asp Val Ser Glu Lys Arg Ala Ile

530 535 540

Met Ala Glu Ile Thr Leu Lys Gly Leu Gly Gly Leu Asn Asn Lys Leu

545 550 555 560

Ala Ser Leu Leu Glu Asn Arg Phe Leu Gly Phe Asn Lys Ile Asn Gln

565 570 575

Ser Trp Thr Gln Arg

580

<210> 36

<211> 747

<212> PRT

<213> Acinetobacter baumannii

<400> 36

Met Ser Ser Glu Lys Arg Asp Leu Thr Asn Phe Ser Arg Phe Leu Val

1 5 10 15

Asn Ile Asn Tyr Val Asp Phe Pro Glu Tyr Ala Lys Leu Pro Leu Ile

20 25 30

Gln Asn Phe Arg Asp Phe Lys His Phe Lys Thr Ala Ile Asp Trp Ser

35 40 45

Asn Lys Phe Asn Ile Gln Lys Ser Val Asp Gly Arg Ile Leu Leu Ser

50 55 60

Val Leu Tyr Ala Glu Ala Gln Asp Val Ala Asn Ala Lys Asp Gln Leu

65 70 75 80

Ser Lys Ile Asp Ile Lys Gly Leu Thr Ala Asp Gln Leu Val Arg Val

85 90 95

Ala Tyr Ala Tyr Arg Leu Ile Asn Leu Pro Val Asp Ala Leu Ala Thr

100 105 110

Val Glu His Ala Tyr Gln Gln Gln Pro Lys Ser Leu Ser Val Leu Gln

115 120 125

Glu Tyr Val Tyr Asp Leu Ile Ala Ile Gly Ser Tyr Lys Lys Ala Gln

130 135 140

Gln Leu Leu Gln Ala Ser Glu Lys Thr Glu Gln Thr Val Gln Met Leu

145 150 155 160

Lys Thr Leu Gln Val Ser Glu Phe Ser Gln His Ile Asn Asn Ala Ile

165 170 175

Ala Arg Tyr Lys Tyr Leu Asn Arg Glu Gly Leu Ser Asp Ala Glu Ser

180 185 190

Phe Ala Glu Leu Asp Lys Val Leu Glu Gln Gly Gln Lys Met His Gln

195 200 205

Gln Met Asn Pro Ser Asp Pro Asn Tyr Leu Arg Phe Tyr Tyr Asp Tyr

210 215 220

Leu Tyr Gly Leu Asp Phe Arg Gly Arg Ser Lys Ala Val Ile Glu Ser

225 230 235 240

Phe Thr Gln Leu Asn Ile Pro Leu Glu Lys Leu Pro Ala Tyr Val Arg

245 250 255

His Ala Ile Ala Asp Ser Tyr Leu Ala Glu Gln Lys Pro Lys Gln Ala

260 265 270

Glu Phe Ala Tyr Lys Thr Leu Leu Thr Glu Lys Asn Tyr Pro Asp Met

275 280 285

Thr Val Tyr Thr Gly Leu Tyr Tyr Ser Tyr Ile Glu Gln Glu Lys Tyr

290 295 300

Lys Glu Ala Glu Gln Leu Leu Ala Glu Val Asp Arg Leu Ile Pro Thr

305 310 315 320

Tyr Lys Tyr Ser Gln Ala Lys Gly Val Asp Lys Ile Ser His Pro Asp

325 330 335

Arg Asp Asp Tyr Ile Ala Leu Gln Gly Met His Leu Ala Tyr Ala Asn

340 345 350

His Leu Asp Gln Ala Glu Lys His Phe Gln Lys Lys Val Glu Gln Ala

355 360 365

Pro Ala Asn Glu Ser Leu Ile Asn Asn Leu Ala Arg Val Glu Arg Trp

370 375 380

Arg Glu Lys Pro Leu Glu Ala Lys Lys Thr Ile Ser Arg Leu Asn Gly

385 390 395 400

Ile Asp Pro Ile Ala Lys Asp Thr Arg Ile Asn Glu Met Gln Asn Ala

405 410 415

Gln Ala Leu Gly Asp Ile Pro Thr Trp Arg Lys Thr Thr Gln Asn Leu

420 425 430

Val Gln Tyr Tyr Pro Asp Asp Ser Gly Val Ile Lys Ser Arg Lys Glu

435 440 445

Leu Glu Asp Arg Asn Arg Ala Thr Ile Ser His Ser Thr Thr Trp Gly

450 455 460

Gln Ser Lys Ala Asp Gly Arg Asp Thr Val Ser Gly Gln Asn Gly Leu

465 470 475 480

Lys Asp Arg Glu Met Glu Thr Arg Leu Asn Ser Pro Trp Ile Asn Asp

485 490 495

Asn Tyr Arg Leu Phe Ala Trp His Gln Asp Arg Tyr Gly Glu Tyr Arg

500 505 510

Phe Gly Asp Val His Asp Gln Arg Tyr Gly Val Gly Ala Glu Trp Gln

515 520 525

Ala Asn Arg Lys Ala Leu Ser Ala Ile Val Ser Gln Ser Thr Asp Gly

530 535 540

Gly Gln Ala Gly Val Arg Leu Asp Trp Ser Gln Trp Leu Asn Asp His

545 550 555 560

Trp Gln Tyr Gln Leu Gln Tyr Asn Ser Gln Ala Asp Ile Pro Leu Gln

565 570 575

Ala Leu Asp Ala Gly Glu Asp Gly Gln Ser Tyr Arg Ala Ala Val Thr

580 585 590

Trp Gln Lys Asp Glu Ser Arg Gln Ile Gly Ala Ser Tyr Gly Leu Thr

595 600 605

Asp Ile Ser Asp Gly Asn Lys Gln Gln Glu Phe Ser Thr Phe Trp Arg

610 615 620

Glu Arg Leu Phe Asp Ala Pro His His Ile Thr Tyr Gly Thr Val Arg

625 630 635 640

Gly Phe Tyr Gly Thr Asn Ser Gln Asp Gln Thr Ala Tyr Phe Ser Pro

645 650 655

Ser Ser His Tyr Ser Ala Glu Leu Asn Leu Ser His Asp Trp Val Thr

660 665 670

Trp Arg Glu Tyr Glu Arg Ser Phe Lys Gln His Phe Glu Ala Gly Val

675 680 685

Gly Leu Tyr Lys Gln Ala Asp Tyr Ser Ala Lys Pro Thr Tyr Ser Leu

690 695 700

Gln Tyr Gln His Gln Trp Gln Leu Ser Arg Thr Trp Gln Leu Asn Tyr

705 710 715 720

Gly Ile Gly Trp Gln Tyr His Pro Tyr Asp Gly His Asp Glu Gln His

725 730 735

Thr Tyr Gly Ile Phe Gly Phe Glu Gly Arg Phe

740 745

<210> 37

<211> 381

<212> PRT

<213> Acinetobacter baumannii

<400> 37

Met Leu Gly Asp Trp Asn Gly Gln Arg Thr Ala Leu Gln Ala Gln Gly

1 5 10 15

Tyr Asp Phe Ser Phe Gly Tyr Thr Gly Glu Tyr Ala Gly Ile Leu Asp

20 25 30

Ser Lys Gln Thr Ser Thr His Gly Ser Ala Tyr Thr Gly Gln Leu Ala

35 40 45

Leu Gly Ser His Leu Asp Leu Gly Lys Ile Leu Gly Trp Gln Asp Thr

50 55 60

Glu Ala Gln Ile Thr Leu Thr Tyr Arg Asp Gly Gln Ser Leu Ser Glu

65 70 75 80

His Ser Pro Ala Leu Ala Gly His Gln Ser Ser Val Gln Glu Val Trp

85 90 95

Gly Arg Glu Gln Thr Trp Arg Leu Thr Asp Leu Trp Ile Lys Lys Lys

100 105 110

Phe Leu Asp Gln Lys Leu Asp Val Lys Val Gly Arg Phe Gly Glu Gly

115 120 125

Glu Asp Phe Asn Ser Phe Asp Cys Asp Phe Gln Asn Leu Ala Leu Cys

130 135 140

Gly Ser Gln Val Gly Asn Trp Val Gly Asp Gln Trp Tyr Asn Trp Pro

145 150 155 160

Val Ser Gln Trp Ala Met Arg Val Lys Tyr Asn Leu Gln Pro Asp Leu

165 170 175

Tyr Thr Gln Val Gly Val Tyr Glu Tyr Asn Pro Glu Asn Leu Glu Arg

180 185 190

Gly Lys Gly Phe Asn Leu Ser Thr Asp Gly Ser His Gly Ala Ile Ile

195 200 205

Pro Ala Glu Val Val Trp Ser Pro Lys Leu Gly Val Gln Ser Met Pro

210 215 220

Gly Glu Tyr Arg Leu Gly Tyr Tyr Tyr Ser Thr Ala Asp Ala Lys Glu

225 230 235 240

Ile Ala Asp Ser Thr Lys Thr Ser His Lys Gln Gly Val Trp Val Thr

245 250 255

Ala Lys Gln Lys Leu Phe Gln Pro Ala Asp Gln Thr Asp Arg Gly Leu

260 265 270

Thr Gly Phe Val Asn Leu Thr Phe His Asp Ser Asp Thr Asn Lys Val

275 280 285

Asp Asn Met Gln Asn Ile Gly Leu Val Tyr Lys Gly Leu Leu Asn Gln

290 295 300

Arg Pro Gln Asp Glu Leu Ala Leu Gly Val Ala Arg Ile His Ile Asn

305 310 315 320

Asp Asp Trp Ser Asp Val Gln Ala Lys Glu Tyr Asp Thr Glu Tyr Asn

325 330 335

Thr Glu Leu Tyr Tyr Gly Ile His Ala Thr Asn Trp Leu Thr Ile Arg

340 345 350

Pro Asn Val Gln Tyr Val Arg His Val Gly Ala Leu Lys Asn Gly Asp

355 360 365

Asn Thr Trp Val Gly Gly Ile Lys Phe Ser Thr Ala Phe

370 375 380

<210> 38

<211> 183

<212> PRT

<213> Acinetobacter baumannii

<400> 38

Met Leu Phe Arg Thr Gly Leu Ala Asp Gly Leu Glu Leu Gln Leu Gly

1 5 10 15

Trp Gln Gly Pro Ala Trp Thr Gln Thr Lys Arg Ala Gly Lys Lys Thr

20 25 30

Asp Asn Ser Gly Phe Gly Asp Val Ser Ile Gly Leu Lys Lys Ala Ile

35 40 45

Asp Leu Asn Asp Glu Asn Leu Thr Met Ala Val Leu Ala Glu Ala Val

50 55 60

Ile Ala Thr Gly Asn Asp Glu Phe Thr Ala His Asp Asp Ile Tyr Ser

65 70 75 80

Leu Ser Ser Ala Val Ala Tyr Lys Tyr Asn Asp Leu Leu Asp Thr Ser

85 90 95

Ile Thr Met Arg Tyr Glu Val Gln Asn Ser Asn Trp Ala Val Thr Ala

100 105 110

Ile Pro Thr Ile Asn Tyr Lys Ile Ala Gly Lys Leu Ser Gly Tyr Ser

115 120 125

Glu Phe Val Tyr Arg Lys Ala Glu Ser Gln Asp Tyr Gln Tyr Gly Leu

130 135 140

Gly Thr Gly Leu Val Tyr Ala Val Asn Asn Arg Thr Gln Leu Asp Ala

145 150 155 160

Asn Ile Gly Val Asp Leu Glu Gly Gln Asp Lys Ser Tyr Lys Gly Gly

165 170 175

Leu Gly Met Ala Phe Leu Phe

180

<210> 39

<211> 217

<212> PRT

<213> Acinetobacter baumannii

<400> 39

Met Arg Ala Leu Val Ile Ser Thr Val Val Gly Ala Ala Val Val Leu

1 5 10 15

Ser Gly Cys Gln Thr Thr Gly Asn Asn Leu Gly Gly Val Glu Tyr Asp

20 25 30

Lys Ala Ala Leu Gly Thr Leu Ile Gly Ala Ala Ala Gly Tyr Gly Ile

35 40 45

Ser Lys Ser Asn Ala Asn Ser Ser Arg Gln Asn Asn Arg Ala Ala Ala

50 55 60

Ile Gly Ala Val Leu Gly Ala Ala Gly Gly Leu Tyr Leu Asp Gln Lys

65 70 75 80

Glu Lys Lys Leu Arg Glu Gln Met Ala Gly Thr Gly Val Glu Val Gly

85 90 95

Arg Asn Pro Asp Gly Ser Val Gln Leu Ile Met Pro Gly Ser Ile Thr

100 105 110

Phe Asp Thr Asn Lys Ser Asn Ile Lys Pro Asn Phe Tyr Ala Thr Leu

115 120 125

Asp Lys Val Ala Gln Thr Leu Ala Glu Asp Asn Lys Ser Ala Ile Leu

130 135 140

Val Thr Gly Tyr Thr Asp Asn Thr Gly Asn Asp Ser Ile Asn Ile Pro

145 150 155 160

Leu Ser Gln Ala Arg Ala Gln Ser Val Lys Asn Tyr Leu Ala Gly Lys

165 170 175

Gly Val Pro Ser Ser Arg Ile Asp Ala Gln Gly Tyr Gly Ser Ser Asn

180 185 190

Pro Ile Ala Asp Asn Ser Thr Ala Ser Gly Arg Glu Gln Asn Arg Arg

195 200 205

Val Glu Ile Ser Ile Tyr Ala Lys Gln

210 215

<210> 40

<211> 299

<212> PRT

<213> Acinetobacter baumannii

<400> 40

Met Lys Lys Leu Gly Leu Ala Thr Ala Val Leu Leu Ala Met Thr Gly

1 5 10 15

Ala His Ala Tyr Gln Phe Glu Val Gln Gly Gln Ser Glu Tyr Val Asp

20 25 30

Thr Thr Ala Asn Asp Lys Asn Phe Thr Gly Asp Val Ala Gly Thr Phe

35 40 45

Tyr Leu Lys Asn Val Asp Thr Ala Lys Gly Pro Leu Ala Glu Ala Ala

50 55 60

Phe Leu Asn Gln Ala Ser Ser Val Ser Leu Gly Tyr Ser Tyr Gln Gln

65 70 75 80

Tyr Asp Gln Asn Asn Val Asn Tyr His Ile Gly Thr Tyr Gly Val Lys

85 90 95

Gly Glu Ala Tyr Val Pro Thr Pro Tyr Leu Pro Val Tyr Ala Ser Ala

100 105 110

Thr Tyr Asn His Thr Asp Val Asp Gly Lys Asn Asn Phe Ser Lys Asp

115 120 125

Asp Asn Gly Asp Arg Tyr Ala Leu Glu Val Gly Ala Met Leu Leu Pro

130 135 140

Asn Phe Leu Met Thr Val Gly Tyr Thr Ser Val Ala Asn Gln Phe Ala

145 150 155 160

Leu Asp Asn Phe Gly Ile Ile Gly Asn Gly Ile Tyr Ser Ala Val Asn

165 170 175

Gln Thr Ala Ala Ile Gln Asn Asp Gln Asp Ala Val Thr Ala Arg Ala

180 185 190

Lys Tyr Val Gly Pro Ile Asp Gly Thr Asn Met Ala Ile Gly Phe Glu

195 200 205

Ala Ala Gly Ala Phe Gly Gln Glu Asn Gln Tyr Gly Leu Lys Thr Asp

210 215 220

Leu Tyr Leu Thr Pro Lys Leu Ser Val Gly Ala Thr Phe Val Gly Asn

225 230 235 240

Asp Gly Glu Ala Asp Ile Lys Gly Asn Asp Leu Gly Glu Phe Arg Gln

245 250 255

Ala Trp Gly Gly Asn Val Asn Tyr Phe Ile Thr Pro Ala Leu Ala Val

260 265 270

Gly Ala Ser Tyr Met Lys Ala Asp Val Lys Lys Ser Ser Tyr Asp Thr

275 280 285

Gln Thr Ile Gly Leu Asn Ala Lys Phe Arg Phe

290 295

<210> 41

<211> 228

<212> PRT

<213> Acinetobacter baumannii

<400> 41

Asp Glu Ala Val Val His Asp Ser Tyr Ala Phe Asp Lys Asn Gln Leu

1 5 10 15

Ile Pro Val Gly Ala Arg Ala Glu Val Gly Thr Thr Gly Tyr Gly Gly

20 25 30

Ala Leu Leu Trp Gln Ala Asn Pro Tyr Val Gly Leu Ala Leu Gly Tyr

35 40 45

Asn Gly Gly Asp Ile Ser Trp Thr Asp Asp Val Ser Val Asn Gly Thr

50 55 60

Lys Tyr Asp Leu Asp Met Asp Asn Asn Asn Val Tyr Leu Asn Ala Glu

65 70 75 80

Ile Arg Pro Trp Gly Ala Ser Thr Asn Pro Trp Ala Gln Gly Leu Tyr

85 90 95

Ile Ala Ala Gly Ala Ala Tyr Leu Asp Asn Asp Tyr Asp Leu Ala Lys

100 105 110

Arg Ile Gly Asn Gly Asp Thr Leu Ser Ile Asp Gly Lys Asn Tyr Gln

115 120 125

Gln Ala Val Pro Gly Gln Glu Gly Gly Val Arg Gly Lys Met Ser Tyr

130 135 140

Lys Asn Asp Ile Ala Pro Tyr Leu Gly Phe Gly Phe Ala Pro Lys Ile

145 150 155 160

Ser Lys Asn Trp Gly Val Phe Gly Glu Val Gly Ala Tyr Tyr Thr Gly

165 170 175

Asn Pro Lys Val Glu Leu Thr Gln Tyr Asn Leu Ala Pro Val Thr Gly

180 185 190

Asn Pro Thr Ser Ala Gln Asp Ala Val Asp Lys Glu Ala Asn Glu Ile

195 200 205

Arg Asn Asp Asn Lys Tyr Glu Trp Met Pro Val Gly Lys Val Gly Val

210 215 220

Asn Phe Phe Trp

225

<210> 42

<211> 609

<212> PRT

<213> Acinetobacter baumannii

<400> 42

Met Gly Phe Asn Ser Ile Gln Ser Gly Gly Thr Asn Gly Asp Val Thr

1 5 10 15

Phe Arg Gly Met Phe Gly Ser Arg Ile Lys Ile Leu Thr Asp Gly Thr

20 25 30

Glu Asn Leu Gly Ala Cys Pro Asn Arg Met Asp Ala Pro Thr Ser Tyr

35 40 45

Ile Ser Pro Glu Ser Tyr Asp Arg Ile Ser Val Ile Lys Gly Pro Gln

50 55 60

Thr Val Gln Tyr Ala Asn Thr Gly Ser Ala Ala Thr Val Leu Phe Glu

65 70 75 80

Arg Gln Pro Glu Lys Leu Thr Ser Glu Lys Pro Tyr Arg Gly Gln Ala

85 90 95

Ser Val Leu Leu Gly Ser Tyr Gly Arg Ile Asp His Asn Ile Glu Ala

100 105 110

Ala Val Gly Asp Glu Lys Lys Tyr Ile Arg Leu Asn Ala Asn Arg Ser

115 120 125

Glu Ser Asn Ser Tyr Gln Asp Gly Asp Gly Asn Thr Val Pro Ser Ala

130 135 140

Trp Lys Lys Trp Asn Ala Asp Val Ala Leu Gly Phe Thr Pro Asp Glu

145 150 155 160

Asn Thr Trp Val Glu Ile Thr Gly Gly Lys Ser Asp Gly Glu Ser Leu

165 170 175

Tyr Ala Gly Arg Ser Met Asp Gly Ser Gln Phe Ala Arg Glu Ser Leu

180 185 190

Gly Leu Arg Phe Glu Lys Lys Asn Ile Thr Asp Val Ile Lys Lys Ile

195 200 205

Glu Gly Gln Val Asn Tyr Ser Tyr Asn Asp His Val Met Asp Asn Phe

210 215 220

Arg Leu Arg Ile Pro Pro Met Thr His Asp Met Met Thr His Gln Met

225 230 235 240

Val Val Asn Pro Ser Glu Met Gln Val Thr Arg Arg Thr Leu Asn Thr

245 250 255

Arg Phe Ala Met Thr Ser Glu Trp Gly Lys Leu Asn Val Ile Thr Gly

260 265 270

Ile Asp Ser Gln Gln Asn His His Ala Glu Ser Met Lys Ser Leu Met

275 280 285

Met Asp Met Pro Leu Thr Thr Asn Met Lys Phe Gln Ser Tyr Gly Ala

290 295 300

Phe Gly Glu Leu Gly Tyr Gln Leu Ser Glu Asn Ser Lys Leu Val Thr

305 310 315 320

Gly Ala Arg Leu Asp Gln Val Lys Ile Asp Ala Leu Lys Leu Asn Asp

325 330 335

Asp Arg Ser Glu Thr Leu Pro Ser Gly Phe Ile Arg Leu Glu Thr Gln

340 345 350

Leu Pro Glu His Asn Ala Lys Ser Tyr Ile Gly Leu Gly Tyr Val Glu

355 360 365

Arg Val Pro Asp Tyr Trp Glu Leu Phe Ser Thr Ala His Gly Asn Ser

370 375 380

Gly Met Pro Lys Pro Thr Phe Asn Asp Leu Asp Thr Glu Lys Thr Leu

385 390 395 400

Gln Leu Asp Met Gly Tyr Gln His Gln His Gly Ala Phe Asn Ser Trp

405 410 415

Ala Ser Ala Tyr Val Gly Leu Ile Asn Asp Tyr Ile Leu Met Ser Tyr

420 425 430

His Asn His Pro Thr Ser Gly Gly His Gly His Gly Ser Ser Phe Ser

435 440 445

Ala Gly Ala Lys Asn Val Asp Ala Thr Ile Ala Gly Ala Glu Ala Gly

450 455 460

Ile Gly Tyr Gln Phe Thr Asp Arg Ile Gln Ala Asp Leu Ser Ala Met

465 470 475 480

Tyr Ala Trp Gly Lys Asn Thr Thr Asp Asp Lys Pro Leu Pro Gln Ile

485 490 495

Ser Pro Leu Glu Gly Arg Leu Asn Ile Arg Tyr Val Ala Asp Lys Tyr

500 505 510

Asn Leu Gly Leu Leu Trp Arg Ala Val Ala Glu Gln Asn Arg Val Ser

515 520 525

Leu His Gln Gly Asn Ile Val Gly Tyr Asp Leu Lys Pro Ser Lys Gly

530 535 540

Phe Ser Thr Leu Ser Leu Asn Gly Ser Tyr Asn Leu Arg Lys Asp Ile

545 550 555 560

Asp Val Ser Val Gly Ile Asp Asn Val Leu Asp Lys Thr Tyr Thr Glu

565 570 575

His Leu Asn Lys Ala Gly Ser Ala Gly Phe Gly Phe Ala Ser Glu Glu

580 585 590

Gln Phe Asn Asn Ile Gly Arg Asn Tyr Trp Val Arg Met Ser Met Lys

595 600 605

Phe

<210> 43

<211> 170

<212> PRT

<213> Acinetobacter baumannii

<400> 43

Met Thr Gly Cys Ala Ser Arg Lys Pro Ala Thr Thr Ala Thr Thr Gly

1 5 10 15

Thr Thr Asn Pro Ser Thr Val Asn Thr Thr Gly Leu Ser Glu Asp Ala

20 25 30

Ala Leu Asn Ala Gln Asn Leu Ala Gly Ala Ser Ser Lys Gly Val Thr

35 40 45

Glu Ala Asn Lys Ala Ala Leu Ala Lys Arg Val Val His Phe Asp Tyr

50 55 60

Asp Ser Ser Asp Leu Ser Thr Glu Asp Tyr Gln Thr Leu Gln Ala His

65 70 75 80

Ala Gln Phe Leu Met Ala Asn Ala Asn Ser Lys Val Ala Leu Thr Gly

85 90 95

His Thr Asp Glu Arg Gly Thr Arg Glu Tyr Asn Met Ala Leu Gly Glu

100 105 110

Arg Arg Ala Lys Ala Val Gln Asn Tyr Leu Ile Thr Ser Gly Val Asn

115 120 125

Pro Gln Gln Leu Glu Ala Val Ser Tyr Gly Lys Glu Ala Pro Val Asn

130 135 140

Pro Gly His Asp Glu Ser Ala Trp Lys Glu Asn Arg Arg Val Glu Ile

145 150 155 160

Asn Tyr Glu Ala Val Pro Pro Leu Leu Lys

165 170

<210> 44

<211> 480

<212> PRT

<213> Acinetobacter baumannii

<400> 44

Met Phe Leu Arg Lys Thr Leu Ser Ile Ala Leu Leu Ala Thr Ala Ser

1 5 10 15

Ser Ala Val Phe Ala Gln Gly Leu Val Leu Asn Asn Asp Asp Leu Arg

20 25 30

Thr Asp Leu Asn Trp Leu Asn Gln Gln Gly Val Ile Asn Ile Ser Thr

35 40 45

Ser Thr Trp Pro Leu Ser Gly Asp Glu Ile Gln Arg Ala Leu Ser Gln

50 55 60

Ala Lys Val Thr His Pro Ala Gln Gln Lys Val Ile Asn Ser Val Leu

65 70 75 80

Asn Ala Leu Lys Ala Asp Asn Asn Thr Val Lys Val Gly Ala Phe Ala

85 90 95

Glu Ser Asp Ile Lys Asn Ile Pro Gln Ala Phe Gly Asp Asn Gln Lys

100 105 110

Ser Gln Tyr Gln Gly Ser Leu Glu Phe Asn Ala Gly Gly Glu Asn Trp

115 120 125

Asp Ala Lys Ile Arg Val Asn Ala Glu Lys Asp Pro Gln Ile Asp Ser

130 135 140

Gly His Asp Val Asn Val Glu Gly Ser Tyr Val Ala Gly Lys Leu Trp

145 150 155 160

Asn Gln Trp Ile Val Ala Gly Gln Ile Pro Thr Trp Trp Gly Pro Gly

165 170 175

His Asp Gly Ser Leu Ile Arg Gly Asp Ala Ser Arg Pro Val Tyr Gly

180 185 190

Val Thr Ala Gln Arg Ala Val Gln Asn Ala Phe Glu Thr Lys Trp Leu

195 200 205

Ser Trp Ile Gly Pro Trp Gln Tyr Gln Ala Phe Ala Gly Gln Leu Asp

210 215 220

Asp Tyr Lys Ala Val Pro Asp Ala Lys Leu Ile Gly Leu Arg Leu Thr

225 230 235 240

Ala Gln Pro Leu Pro Tyr Leu Glu Leu Gly Ala Ser Arg Thr Ile Gln

245 250 255

Trp Gly Gly Asp Gly Arg Ser Glu Ser Phe Ser Ser Leu Trp Asp Ala

260 265 270

Ile Lys Gly Asn Asp Asn Val Tyr Gly Asp Thr Glu Asn Pro Ser Asn

275 280 285

Gln Leu Ala Gly Phe Asp Gly Arg Leu Leu Leu Gln Pro Leu Leu Asn

290 295 300

Ile Pro Val Ser Leu Tyr Gly Gln Tyr Val Gly Glu Asp Glu Ala Gly

305 310 315 320

Tyr Leu Pro Ser Lys Lys Met Tyr Leu Ala Gly Val Asp Tyr Ser Ser

325 330 335

Ser Tyr Asn Asp Met Pro Tyr Gln Leu Tyr Ala Glu Trp Ala Asp Thr

340 345 350

Arg Thr Asn Gly Asp Val Lys Ser Ile Ser Tyr Thr His Ser Val Tyr

355 360 365

Lys Asp Gly Tyr Tyr Gln His Gly Phe Pro Leu Gly His Ala Met Gly

370 375 380

Gly Asp Gly Gln Met Tyr Ser Val Gly Gly Asp Ile Arg Phe Asp Val

385 390 395 400

Met Asn Arg Leu Ser Gly Arg Ala Met Val Val Lys Val Asn Gln Ser

405 410 415

Asn Leu Ala Ile Asn Lys Ala Phe Pro Lys Asp Asp Glu Ile Lys Ala

420 425 430

Leu Asp Leu Thr Trp Thr His Tyr Ile Lys Pro Asp Leu Pro Leu Lys

435 440 445

Ile Asn Gly Trp Val Ser Asp Ser Asp Leu Glu Gly Asn Asp Ala Gly

450 455 460

Ala Ser Ile Gly Val Glu Ile Pro Leu Glu Arg Lys Met Phe Gly Phe

465 470 475 480

<210> 45

<211> 114

<212> PRT

<213> Acinetobacter baumannii

<400> 45

Met Asn Lys Leu Leu Val Ala Leu Gly Leu Ala Ala Thr Val Ala Leu

1 5 10 15

Val Gly Cys Asn Lys Asp Lys Ala Pro Glu Thr Gly Ala Thr Thr Gly

20 25 30

Glu His Leu Glu Asn Ala Ala Gln Gln Ala Thr Ala Asp Ile Lys Ser

35 40 45

Ala Gly Asp Gln Ala Ala Ser Asp Ile Ala Thr Ala Thr Asp Asn Ala

50 55 60

Ser Ala Lys Ile Asp Ala Ala Ala Asp His Ala Ala Asp Ala Thr Ala

65 70 75 80

Lys Ala Ala Ala Glu Thr Glu Ala Thr Ala Arg Lys Ala Thr Ala Asp

85 90 95

Thr Ala Gln Ala Val Glu Asn Ala Ala Ala Asp Val Lys Lys Asp Ala

100 105 110

Gln His

<210> 46

<211> 725

<212> PRT

<213> Acinetobacter baumannii

<400> 46

Met Tyr Glu Tyr Pro Ser Tyr Asp Tyr Arg Gly Asn Phe Lys Ile Thr

1 5 10 15

Val Asp Pro Ser Gln Ile Lys Gln Asn Val Lys Ala Glu Asn Thr Ala

20 25 30

Lys Leu Asp Ala Glu Leu Gln Lys Lys Val Asp Gln Tyr Leu Arg Glu

35 40 45

Gln Lys Val Ala Leu Ser Lys Ala Gln Lys Gln Thr Leu Tyr Ala Ala

50 55 60

Ile Ala Asn Glu Gln Gly Asp Trp Gly Leu Thr Ser Ser Ala Arg Ser

65 70 75 80

Glu Lys Ile Asn Asn Ile Leu Ile Asn Leu Leu Asn Asp Leu Gln Phe

85 90 95

Ser Tyr Asp Gly Ser Ile His Tyr Arg Gln Lys Met Gly Ser Phe Asn

100 105 110

Leu Thr Ala Arg Tyr Glu Lys Pro Thr Leu Leu Val Gln Ala Lys Leu

115 120 125

Pro Met Val Leu Asp Leu Glu Asn Tyr Lys Phe Tyr Ile Asn Tyr Phe

130 135 140

Gly Leu Met Pro Tyr Leu Val Asn Lys Asp Asn Gln Asn Asn Leu Ala

145 150 155 160

Tyr Val Asp Phe Ser Lys Tyr Lys Ala Phe Phe Lys Asn Val Asp Lys

165 170 175

Lys Lys Phe Ile Glu Tyr Leu Lys Ala Ser Ser Ala Val Ser Tyr Arg

180 185 190

Leu Ala Glu Pro Gln Asn Leu Gln Arg Val Ser Leu Thr Glu Ala Asp

195 200 205

Arg Lys Ala Gly Ala Val Glu Arg Ile Arg Leu Lys Thr Thr Val Glu

210 215 220

Gln Leu Leu Leu Glu Val Asp Leu Phe Gly Gln Val Asn Glu Lys Tyr

225 230 235 240

Leu Gln Lys Ser Val Leu Gly Leu Asp Glu Glu Lys Leu Ala Glu Thr

245 250 255

Leu Ala Ala Glu Ile Ala Ala Ser Asp Ala Lys Lys Gly Thr Ala Gly

260 265 270

Lys Glu Glu Gln Lys Val Ser Ser Asp Asp Ala Ala Ala Val Ser Gln

275 280 285

Gln Leu Tyr Ser Leu Val Asn Ala His Leu Gly Asn Thr Ser Thr Ser

290 295 300

Glu Asp Glu Glu Val Glu Ser Ala Ser Ser Glu Glu Ala Ser Asp Val

305 310 315 320

Ala Val Ala Glu Ala Glu Gln Thr Ser Glu Asn Glu Glu Val Val Val

325 330 335

Leu Thr Glu Asp Gln Cys Ile Glu Leu Lys Ser Leu Lys Asn Pro Val

340 345 350

Ala Leu Gly Asp Ile Asn Tyr Cys Gln Ile Tyr Gly Ile Asp Val Leu

355 360 365

Asp Gln Ser Asp Thr Ser Ile Gln Lys Ala Gln Ile Lys Ser Arg Gln

370 375 380

Asp Ala Leu Lys Gln Thr Phe Glu Val Tyr Asn Gln Asn Gln Phe Ile

385 390 395 400

Asn Asp Glu Ala Phe Lys Val Leu Trp Leu Lys His Lys Asp Glu Ile

405 410 415

Glu Gln Ala Leu Pro Lys Gln Arg Asn Pro Ile Thr Ile Asp Val Ala

420 425 430

Leu Asp Asp Lys Gly Arg Ala Val Asn Met Asp Tyr Asp Val Asp Tyr

435 440 445

Thr Pro Ala Glu Phe Lys His Arg Phe Asn Ile Lys Ala Asp Met Gln

450 455 460

Ile Leu Asn Tyr Gly Lys Ala Thr Ser Ile Asp Gln Gln Gln Leu Lys

465 470 475 480

Gln Ala Lys Ser Val Ala Glu Ala Ser Lys Gly Ser Met Leu Glu Asn

485 490 495

Ile Ile Lys Gly Phe Ser Glu Lys Leu Gly Gln Ser Asp Val Ser Glu

500 505 510

His Pro Val Gly Thr His Ser Asp Val Gln Asp Leu Asp Ala Asn Leu

515 520 525

Ala Ile Leu Ala Asp Lys Thr Tyr Asp Ala Thr His Ala Tyr Asp Lys

530 535 540

Thr Tyr Lys Ala Val Phe Ile Ala Lys Leu Thr Ala Glu Lys Pro Ser

545 550 555 560

Tyr Ile Lys Tyr Tyr Ser Val Gln Gln Leu Gln Glu Ile Ala Glu Val

565 570 575

Tyr Ala Tyr Trp Phe Ser Asp Glu Asp Thr Tyr Asn Pro Gln Gly Lys

580 585 590

Ala Leu Glu Arg Ile Thr Ala Leu Gln Lys Lys His His Leu Glu Gln

595 600 605

Asp Asp Gln Phe Asp His Glu Leu Gly Arg Ala Val Asp His Ile Val

610 615 620

Leu Thr Thr Ile Gln Gly Lys Thr Gly Arg Glu Ala Trp Gln Arg Leu

625 630 635 640

Gln Lys Gln Tyr Lys Gln Pro Ala Gln Leu Phe Ser Lys Gln Tyr Gln

645 650 655

Leu Glu Phe Glu Lys Gln Asn Gly Val Ser Ala Glu Glu Lys His Leu

660 665 670

Leu Ser Glu Thr Ala Asp Ile Leu Gly Asn Val Tyr Val Ala Ala His

675 680 685

Lys Lys Gln Leu Ser Glu Lys Thr Ile Gln Asn Leu Lys Pro Glu His

690 695 700

Asn Glu Phe Ile Asp Tyr Glu Ile Phe Arg Glu Val Tyr Lys Gln Met

705 710 715 720

Val Ala Ala Arg Lys

725

<210> 47

<211> 746

<212> PRT

<213> Acinetobacter baumannii

<400> 47

Met Thr Pro Cys Cys Leu Ala Ile Ser Ala Ile Phe Ala Gln Gln Ala

1 5 10 15

Tyr Ala Glu Thr Val Thr Gln Thr Ala Glu Val Ser Glu Asn Ala Thr

20 25 30

Gln Lys Pro Val Ala Gln Leu Gln Lys Ile Val Val Thr Ala Thr Arg

35 40 45

Thr Pro Lys Asn Ile Ala Glu Ile Ala Gly Thr Val Gln Ser Ile Asp

50 55 60

Gln Lys Gln Ile Ile Gln Gln Ala Thr Ala Gly Arg Lys Val Ala Asp

65 70 75 80

Ile Leu Ala Gln Leu Val Pro Ser Leu Ala Ser Ser Ser Gly Thr Thr

85 90 95

Ser Asn Tyr Gly Gln Thr Met Arg Gly Arg Asn Val Leu Val Met Ile

100 105 110

Asp Gly Val Ser Gln Thr Gly Ser Arg Asp Val Ser Arg Gln Leu Asn

115 120 125

Ser Ile Ser Pro Gly Met Ile Glu Arg Ile Glu Val Ile Ser Gly Ala

130 135 140

Thr Ser Ile Tyr Gly Ser Gly Ala Thr Gly Gly Ile Ile Asn Ile Ile

145 150 155 160

Thr Lys Arg Ala Asp Thr Ser Lys Pro Leu Ser Phe Glu Thr Lys Val

165 170 175

Gly Ile Thr Ser Ser Asp Thr Phe Arg Ser Asp Gly Leu Ala Tyr Glu

180 185 190

Val Gly Gln Ser Val Ser Phe Asn Lys Gly Asn Ile Asp Gly Phe Leu

195 200 205

Gly Ala Asn Phe Thr Ser Arg Gly Ser Gln Phe Asp Gly Asn Gly Asp

210 215 220

Arg Ile Ser Leu Ser Pro Trp Gln Gly Ser Thr Met Asp Thr Asp Thr

225 230 235 240

Ile Asp Val Asn Gly Arg Leu Asn Phe Asn Leu Asn Asp Thr Gln Thr

245 250 255

Leu Ser Phe Gly Ala Gln Tyr Tyr Lys Asp Lys Gln Asp Thr Asp Tyr

260 265 270

Gly Pro Asp Tyr Ser Tyr Leu Pro Thr Thr Ser Lys Ser Asn Asp Ala

275 280 285

Thr Thr Pro Thr Tyr Lys Ala Ile Lys Gly Leu Lys Leu Ser Asn Pro

290 295 300

Leu Phe Thr Glu Arg Tyr Ala Val Asn Ser Gln Tyr Gln Asn Gln Asp

305 310 315 320

Phe Leu Gly Gln Ile Leu Asn Val Glu Ala Tyr Tyr Arg Asn Glu Lys

325 330 335

Ser Arg Phe Phe Pro Tyr Gly Leu Ser Asn Lys Ser Val Thr Ser Val

340 345 350

Asn Gln Ser Gln Ser Glu Ile Glu Val Ala Gly Leu Arg Ser Thr Met

355 360 365

Gln Thr Asp Leu Asn Ile Ala Asn Arg Asp Met Lys Ile Thr Tyr Gly

370 375 380

Leu Asp Tyr Asp Trp Glu Lys Asp Lys Gln Phe Val Asp Ile Leu Ala

385 390 395 400

Thr Gln Tyr Pro Tyr Leu Val Tyr Thr Pro Thr Gly Gln Arg Lys Gly

405 410 415

Tyr Gly Pro Asn Thr Glu Ile Gln Asn Ile Gly Ala Phe Val Gln Ser

420 425 430

Asp Tyr Ala Val Thr Asp Lys Leu Asn Leu Gln Ala Gly Ile Arg Tyr

435 440 445

Gln Tyr Ile Gln Ala Asp Thr Asp Ala Tyr Ile Pro Ser Arg Glu Thr

450 455 460

Thr Met Val Pro Ala Gly Ser Thr His Asp Asp Lys Pro Leu Phe Asn

465 470 475 480

Leu Gly Ala Val Tyr Lys Leu Thr Asp Ala Gln Gln Val Tyr Ala Asn

485 490 495

Phe Ser Gln Gly Phe Ser Phe Pro Asp Val Gln Arg Met Leu Arg Asp

500 505 510

Val Ser Thr Tyr Thr Val Ser Thr Ala Asn Leu Gln Pro Ile Thr Val

515 520 525

Asn Ser Tyr Glu Leu Gly Trp Arg Leu Asn Gln Asp Asp Gly Leu Asn

530 535 540

Leu Gly Leu Thr Gly Phe Tyr Asn Thr Ser Asp Lys Thr Val Gln Phe

545 550 555 560

Asn Asn Arg Ala Ala Lys Val Val Asp Thr Asp Gln Arg Val Tyr Gly

565 570 575

Ala Glu Ala Thr Ile Ser Tyr Pro Phe Met Glu Asn Tyr Lys Val Gly

580 585 590

Gly Thr Leu Gly Tyr Thr Arg Gly Gln Tyr Lys Asp Val Ala Asn Lys

595 600 605

Trp His Glu Leu Asn Ser Phe Thr Val Ala Pro Val Lys Gly Thr Leu

610 615 620

Phe Ala Glu Trp Asp Asn Asn Glu Gly Tyr Gly Val Arg Val Gln Met

625 630 635 640

Gln Ala Ile Lys Gly Thr Asn Lys Ala Tyr Lys Asp Asp Arg Glu Leu

645 650 655

Ala Ala Phe Ala Thr Thr Gln Asp Glu Ala Phe Gln Asn Ala Val Lys

660 665 670

Asn Asp Ala Asn Ser Ala Ala Gln Ile Lys Gly Tyr Thr Thr Met Asp

675 680 685

Val Leu Ala His Phe Pro Ala Trp Lys Gly Arg Val Asp Phe Gly Val

690 695 700

Tyr Asn Val Trp Asn Arg Gln Tyr Arg Thr Val Phe Ala Gln Gln Ala

705 710 715 720

Ala Val Ser Asn Ala Asn Pro Leu Leu Ala Ile Pro Ala Glu Gly Arg

725 730 735

Thr Tyr Gly Leu Ser Tyr Thr Phe Asn Tyr

740 745

<210> 48

<211> 476

<212> PRT

<213> Acinetobacter baumannii

<400> 48

Met Gly Ala Gln Leu Val Arg Glu Val Ser Ser Lys Thr Asn Asp Ile

1 5 10 15

Ala Gly Asp Gly Thr Thr Thr Ala Thr Val Leu Ala Gln Ala Ile Leu

20 25 30

Asn Glu Gly Ile Lys Ser Val Thr Ala Gly Met Asn Pro Met Asp Leu

35 40 45

Lys Arg Gly Ile Asp Ile Ala Val Lys Thr Val Val Glu Asn Ile Arg

50 55 60

Ser Ile Ala Lys Pro Ala Asp Asp Phe Lys Ala Ile Glu Gln Val Gly

65 70 75 80

Ser Ile Ser Ala Asn Ser Asp Thr Thr Val Gly Lys Leu Ile Ala Gln

85 90 95

Ala Met Glu Lys Val Gly Lys Glu Gly Val Ile Thr Val Glu Glu Gly

100 105 110

Ser Gly Phe Glu Asp Ala Leu Asp Val Val Glu Gly Met Gln Phe Asp

115 120 125

Arg Gly Tyr Ile Ser Pro Tyr Phe Ala Asn Lys Gln Asp Thr Leu Thr

130 135 140

Ala Glu Leu Glu Asn Pro Phe Ile Leu Leu Val Asp Lys Lys Ile Ser

145 150 155 160

Asn Ile Arg Glu Leu Ile Ser Val Leu Glu Ala Val Ala Lys Thr Gly

165 170 175

Lys Pro Leu Leu Ile Ile Ala Glu Asp Val Glu Gly Glu Ala Leu Ala

180 185 190

Thr Leu Val Val Asn Asn Met Arg Gly Ile Ile Lys Val Cys Ala Val

195 200 205

Lys Ala Pro Gly Phe Gly Asp Arg Arg Lys Ala Met Leu Gln Asp Ile

210 215 220

Ala Ile Leu Thr Gly Ala Thr Val Ile Ser Glu Glu Val Gly Met Ser

225 230 235 240

Leu Glu Gln Ala Thr Leu Gln Asp Leu Gly Thr Ala His Lys Ile Thr

245 250 255

Val Ser Lys Glu Asn Thr Val Ile Val Asp Gly Ala Gly Asp Ala Ala

260 265 270

Ala Ile Ala Glu Arg Val Gln Gln Ile Arg Ala Gln Ile Glu Glu Ser

275 280 285

Thr Ser Glu Tyr Asp Arg Glu Lys Leu Gln Glu Arg Val Ala Lys Leu

290 295 300

Ala Gly Gly Val Ala Val Ile Lys Ile Gly Ala Ala Thr Glu Val Glu

305 310 315 320

Met Lys Glu Lys Lys Asp Arg Val Asp Asp Ala Leu His Ala Thr Arg

325 330 335

Ala Ala Val Glu Glu Gly Val Val Ala Gly Gly Gly Val Ala Leu Val

340 345 350

Arg Ala Val Asn Ala Leu Glu Gly Leu Lys Gly Ala Asn Glu Asp Gln

355 360 365

Thr Ala Gly Ile Asn Ile Leu Arg Arg Ala Ile Glu Ala Pro Leu Arg

370 375 380

Gln Ile Val Ala Asn Ala Gly Asp Glu Pro Ser Val Val Ile Asn Ala

385 390 395 400

Val Lys Asn Gly Glu Gly Asn Phe Gly Tyr Asn Ala Ala Thr Gly Glu

405 410 415

Tyr Gly Asp Met Leu Glu Met Gly Ile Leu Asp Pro Ala Lys Val Thr

420 425 430

Arg Ser Ala Leu Glu His Ala Ala Ser Val Ala Gly Leu Met Leu Thr

435 440 445

Thr Glu Cys Met Ile Thr Asp Ile Pro Glu Asp Lys Pro Ala Ala Pro

450 455 460

Asp Met Gly Gly Met Gly Gly Met Gly Gly Met Met

465 470 475

<210> 49

<211> 472

<212> PRT

<213> Acinetobacter baumannii

<400> 49

Met Ser Ala Phe Phe Gln Pro Gly Asn Tyr Phe Glu Ala Gly Ile Ser

1 5 10 15

Val Leu Asp Pro Asp Val Ala Gly Lys Glu Ala Gly Ser Ser Ala Thr

20 25 30

Arg Arg Asp Ile Gly Asp Met Ala Asn Asp Tyr Tyr Phe Pro Ser Ala

35 40 45

Ala Leu Lys Leu Gln Ile Asn Asp Gln Phe Ser Phe Gly Leu Leu Tyr

50 55 60

Asp Gln Pro Phe Gly Ala Asp Ala Glu Tyr Ser Gly Asn Asn Val Phe

65 70 75 80

Val Ser Asn Pro Gly Ser Asp Thr Ile Leu Ser Gln Lys Ala Leu Gly

85 90 95

Asp Leu Ala Thr Ser Ser Ile Gln Lys Leu Val Gln Ala Ser Gly Ser

100 105 110

Ala Phe Thr Pro Ala Leu Ile Glu Val Thr Lys Val Thr Gly Gly Asp

115 120 125

Pro Thr Lys Pro Thr Gln Thr Glu Ile Leu Gly Ala Leu Gln Gln Val

130 135 140

Ala Ala Gly Gly Asn Thr Thr Val Gly Ala Gly Leu Thr Ala Leu Gln

145 150 155 160

Lys Thr Gln Ala Ala Ile Asn Ala Ala Asn Asn Tyr Leu Gly Thr Gly

165 170 175

Gly Thr Lys Val Lys Val Asp Thr Gln Asn Leu Ser Phe Val Phe Gly

180 185 190

Tyr Gln Pro Thr Lys Asn Phe Asn Phe Tyr Ala Gly Pro Val Leu Gln

195 200 205

Thr Val Lys Gly Asn Val Ser Leu Arg Gly Gln Ala Tyr Ser Leu Tyr

210 215 220

Asn Gly Tyr Asp Ala Asn Ile Lys Glu Thr Thr Gly Ala Gly Trp Leu

225 230 235 240

Ala Gly Ala Ala Tyr Gln Ile Pro Glu Ile Ala Leu Arg Ala Ser Val

245 250 255

Thr Tyr Arg Ser Glu Ile Asp His Lys Val Asn Ile Asp Glu Asn Leu

260 265 270

Ser Ile Leu Asn Phe Pro Gly Leu Thr Ser Val Leu Ala Gly Leu Asp

275 280 285

Val Pro Ala Ser Lys Leu Gln Ala Ile Asn Ser Ser Gly Lys Thr Thr

290 295 300

Ile Thr Thr Pro Gln Ser Val Asn Leu Asp Phe Gln Thr Gly Ile Met

305 310 315 320

Ala Asp Thr Val Ala Phe Ala Asn Val Arg Trp Val Asn Trp Lys Asp

325 330 335

Phe Ser Ile Gln Pro Tyr Lys Phe Gly Lys Val Ser Glu Ala Val Gly

340 345 350

Gly Leu Ile Gly Arg Pro Asn Gly Phe Asn Leu Val Glu Tyr Ser Asp

355 360 365

Asp Gln Trp Ser Val Asn Ala Gly Val Gly Arg Lys Leu Asn Asp Lys

370 375 380

Trp Ala Gly Asn Val Ser Val Gly Trp Asp Ser Gly Ala Gly Asn Pro

385 390 395 400

Val Thr Thr Leu Gly Pro Thr Glu Gly Tyr Trp Asn Val Gly Leu Gly

405 410 415

Val Gln Tyr Ser Pro Thr Pro Gln Thr Phe Ile Ala Gly Gly Val Lys

420 425 430

Tyr Phe Trp Leu Gly Asp Ala Lys Ala Gln Thr Gly Ala Gln Ala Gly

435 440 445

Ser Asp Glu Tyr Val Ala Asp Phe Ser Asp Asn Asn Ala Ile Ala Tyr

450 455 460

Gly Leu Lys Leu Gly Tyr Lys Phe

465 470

<210> 50

<211> 216

<212> PRT

<213> Acinetobacter baumannii

<400> 50

Met Val Phe Ala Gln Asp Asp Asn Ala Leu His Tyr Asn Ile Val Asn

1 5 10 15

Val Gln Ala Glu Ala Ser Arg Gln Val Ser Asn Asp Glu Met His Ala

20 25 30

Thr Leu Tyr Ile Glu Lys Ser Asn Lys Gln Pro Ala Glu Leu Ser Asn

35 40 45

Gln Ile Asn Gln Leu Met Asn Gln Ala Leu Ala Thr Ser Arg Lys Tyr

50 55 60

Pro Gln Val Lys Val Glu Thr Gly Ala Gln Ser Thr Tyr Pro Ile Tyr

65 70 75 80

Asp Asn Asp Ser Asn Lys Leu Lys Glu Trp Arg Gly Arg Ala Glu Ile

85 90 95

Arg Leu Glu Ser Lys Asp Phe Lys Ala Ala Ser Gln Leu Ile Asn Glu

100 105 110

Leu Gln Gln Ser Phe Gln Thr Gln Ser Ile Asn Phe Ser Val Ser Asp

115 120 125

Glu Gln Arg Lys Lys Val Glu Asn Glu Leu Met Val Glu Ala Ser Lys

130 135 140

Asn Phe Gln Gln Arg Ala Gln Met Leu Thr Gln Ala Trp Asn Lys Ser

145 150 155 160

Gln Tyr Ser Leu Val Thr Leu Asn Leu Asn Thr Asn Asn Tyr Phe Pro

165 170 175

Gln Pro Val Met Arg Ala Ser Leu Ala Lys Phe Ala Ala Ala Glu Ala

180 185 190

Ala Pro Ala Gln Asp Met Ala Ala Gly Glu Ser Lys Ile Thr Val Asn

195 200 205

Ala Asn Gly Ser Ile Gln Phe Lys

210 215

<210> 51

<211> 366

<212> PRT

<213> Acinetobacter baumannii

<400> 51

Met Lys Tyr Cys Gln Phe Phe Ser Val Leu Ala Leu Ser Leu Ser Ala

1 5 10 15

Ala Ser Cys Ala Val Thr Ser Gly Leu Gln Thr Tyr Asp Ile Pro Ser

20 25 30

Glu Gly Val Tyr Lys Thr Asp Leu Gly Thr Thr Val Asn Val Val Lys

35 40 45

Ile Ser Gln Glu Thr Leu Pro Ala Ile Gln Pro Ala Gln Ile Asp Tyr

50 55 60

Gln Arg Asp Tyr Ala Ser Leu Phe Lys Asn Gln Gln Ser Ile Tyr Arg

65 70 75 80

Leu Ser Pro Gly Asp Val Leu Ser Ile Gln Leu Trp Ala Tyr Pro Glu

85 90 95

Ile Thr Pro Pro Val Asn Asn Ile Ser Asn Glu Gln Ser Ile Gln Ala

100 105 110

Asn Gly Tyr Pro Ile Asp Gln Ser Gly Tyr Ile Gln Phe Pro Leu Val

115 120 125

Gly Arg Tyr Lys Ala Ala Gly Lys Thr Leu Ala Gln Val Asn Arg Glu

130 135 140

Leu His Ser Gln Leu Ala Arg Phe Leu Lys Asn Pro Asp Val Val Val

145 150 155 160

Arg Val Val Ser Tyr Glu Gly Gln Arg Phe Ser Val Gln Gly Ser Val

165 170 175

Thr Lys Gly Gly Gln Phe Tyr Leu Ser Asp Gln Pro Val Ser Ile Tyr

180 185 190

Thr Ala Leu Gly Met Ala Gly Gly Val Thr Thr Thr Gly Asp Asn Thr

195 200 205

Tyr Ile Gln Leu Ile Arg Asn Gly Arg Thr Tyr Asn Leu Asn Thr Ile

210 215 220

Asp Leu Glu Lys Ala Gly Tyr Ser Leu His Lys Leu Leu Val Gln Pro

225 230 235 240

Asn Asp Thr Ile Tyr Val Ser Thr Arg Glu Asn Gln Lys Ile Tyr Val

245 250 255

Met Gly Glu Ser Gly Lys Asn Gln Ala Leu Pro Met Arg Asp Gln Gly

260 265 270

Met Thr Leu Ser Asp Ala Leu Gly Glu Ser Leu Gly Ile Asn Pro Asn

275 280 285

Ser Ala Ser Ala Ser Arg Ile Tyr Val Val Arg Thr Asn Pro Asn Asp

290 295 300

Arg Thr Thr Glu Ile Tyr His Leu Asn Leu Met Ser Leu Gly Asp Phe

305 310 315 320

Gly Leu Ala Asn Gln Phe Arg Leu Arg Ser Asn Asp Ile Val Tyr Ile

325 330 335

Asp Ala Thr Gly Leu Thr Arg Trp Gln Arg Val Val Asn Gln Ile Ile

340 345 350

Pro Phe Ser Asn Ala Leu Tyr Asn Ile Asp Arg Leu Gly Gln

355 360 365

<210> 52

<211> 208

<212> PRT

<213> Acinetobacter baumannii

<400> 52

Met Leu Asp Arg His Val Leu Arg Pro Val Ala Val Glu Tyr Arg Glu

1 5 10 15

Lys Thr Pro Glu Asp Val Arg Gly Ser Tyr Arg Gln Phe Arg Lys Asn

20 25 30

Leu Gly Glu Pro Trp Asn Ala Val Asn Gln Leu Ile Gln Gly Arg Pro

35 40 45

Gly Arg Ala Ala Lys Thr Leu Gly Arg Phe Thr Ile Asn Thr Leu Thr

50 55 60

Thr Leu Gly Leu Ala Asp Pro Ala Ser Arg Leu Gly Leu Pro Pro Glu

65 70 75 80

Glu Glu Ser Phe Gly Val Thr Leu Gly Tyr Tyr Gly Val Pro Ser Gly

85 90 95

Pro Phe Leu Met Leu Pro Phe Phe Gly Pro Ser Thr Leu Arg Asp Gly

100 105 110

Val Gly Leu Ala Val Asp Ala Gln Ala Arg Pro Gln Lys Tyr Ile Met

115 120 125

Asp Asp Gln Asp Gly Leu Tyr Trp Ser Thr Asn Leu Leu Gln Ala Val

130 135 140

Asp Thr Arg Ala Gln Tyr Leu Asp Leu Asp Gln Thr Ile Gln Gly Asp

145 150 155 160

Gln Tyr Ala Met Ile Arg Asp Leu Tyr Leu Gln Arg Lys Ala Phe Gln

165 170 175

Ile Ala Glu Lys Lys Gly Asp Ser Ala Asp Val Ser Phe Ile Asp Asp

180 185 190

Asp Glu Ser Glu Asp Val Pro Glu Asp Asn Thr Asp Lys Thr Glu Lys

195 200 205

<210> 53

<211> 326

<212> PRT

<213> Acinetobacter baumannii

<400> 53

Met Val Leu Leu Asn Ser Lys Gly Pro Val Gly Gln Gly Gln Ser Asp

1 5 10 15

Leu Met Met Thr Ala Ile Tyr Leu Met Leu Leu Val Val Ile Pro Ser

20 25 30

Ile Ile Met Ala Leu Trp Phe Gly Trp Lys Tyr Arg Ala Ser Asn Lys

35 40 45

Asp Ala Asp Tyr Lys Pro Thr Trp Ala His Ser Thr Ala Ile Glu Val

50 55 60

Val Val Trp Gly Ile Pro Val Ile Ile Ile Gly Ile Leu Ala Trp Leu

65 70 75 80

Thr Trp Trp Gly Ser His Lys Tyr Asp Pro Tyr Arg Pro Leu Glu Ser

85 90 95

Asp Lys Ala Pro Leu Thr Ile Gln Val Ile Ala Glu Gln Phe Lys Trp

100 105 110

Ile Phe Ile Tyr Pro Glu Gln Asn Ile Ala Thr Val Asn Glu Val Arg

115 120 125

Phe Pro Glu Lys Thr Pro Leu Ser Phe Lys Ile Thr Ser Asn Phe Thr

130 135 140

Met Asn Ser Phe Phe Ile Pro Gln Leu Gly Gly Gln Ile Tyr Ala Met

145 150 155 160

Ala Gly Met Gln Thr His Leu His Leu Leu Ala Asn Glu Thr Gly Val

165 170 175

Tyr Arg Gly Phe Ser Ser Asn Tyr Ser Gly Tyr Gly Phe Ser Gln Met

180 185 190

Arg Phe Lys Ala His Ser Val Thr Glu Gln Gln Phe Asn Glu Trp Val

195 200 205

Ala Ala Val Lys Ala Gly Asn Gly Thr Thr Ile Asn Pro Glu Ala Val

210 215 220

Gln Lys Thr Thr Leu Asp Gln Ala Glu Leu Ala Thr Leu Arg Asp Gly

225 230 235 240

Asp Arg Ser Lys His Gln Ile Glu His Leu Val Asn Arg Ala Lys Ala

245 250 255

Ala Gly Asp Gln Glu Ala Leu Ala Lys Ala Glu Ala Met Lys Pro Phe

260 265 270

Pro Thr Lys Pro His Pro Val Thr Tyr Tyr Ser Ser Val Glu Pro Lys

275 280 285

Leu Phe Glu Thr Ile Ile Asn His Tyr Met Ser Asn Tyr His Gly Ala

290 295 300

Asp His Ser Ala Ala His Thr Ala Ala Glu Thr His Val Ala Ala Glu

305 310 315 320

His Ala Ala Gln Gly Glu

325

<210> 54

<211> 207

<212> PRT

<213> Acinetobacter baumannii

<400> 54

Met Ala Arg Tyr Ile Gly Pro Lys Cys Lys Leu Ser Arg Arg Glu Gly

1 5 10 15

Thr Asp Leu Gln Leu Lys Ser Gly Val Lys Pro Phe Asp Val Lys Thr

20 25 30

Lys Lys His Ala Lys Ala Pro Gly Gln His Gly Gln Ala Arg Gly Lys

35 40 45

Gln Ser Glu Tyr Ser Leu Gln Leu Arg Glu Lys Gln Lys Val Arg Arg

50 55 60

Met Tyr Gly Val Leu Glu Arg Gln Phe Ser Asn Tyr Tyr Lys Glu Ala

65 70 75 80

Ala Arg Val Lys Gly Ala Thr Gly Glu Asn Leu Leu Lys Leu Leu Glu

85 90 95

Ser Arg Leu Asp Asn Val Val Tyr Arg Met Gly Phe Gly Ser Thr Arg

100 105 110

Ala Glu Ala Arg Gln Leu Val Ser His Arg Ser Ile Thr Leu Asn Gly

115 120 125

Arg Arg Val Asn Ile Ala Ser Ile Gln Val Lys Ala Gly Asp Val Ile

130 135 140

Ala Val His Glu Gly Ala Lys Gln Gln Leu Arg Ile Lys Asn Ala Ile

145 150 155 160

Glu Leu Ala Ala Gln Arg Gly Ile Pro Ala Trp Met Asp Val Asp His

165 170 175

Ser Lys Leu Glu Gly Thr Phe Lys Ala Ala Pro Asp Arg Ser Asp Leu

180 185 190

Pro Ala Glu Ile Asn Glu Ser Leu Ile Val Glu Leu Tyr Ser Lys

195 200 205

<210> 55

<211> 355

<212> PRT

<213> Acinetobacter baumannii

<400> 55

Met Ala His Ala Glu Val Asn Ser Ser Ser Thr Gln Gln Val Asn Gly

1 5 10 15

Leu Ser Ser Gly Ala Gln Ala Glu Glu Asn Lys Asp Glu Asn Leu Leu

20 25 30

Asp Gln Ile Pro Arg Trp Ile Asp Ala Thr Pro Thr Ile Phe Pro Glu

35 40 45

Gln Ser Asn Glu Pro Ile Val Pro Pro Thr Glu Gln Thr Glu Asp Gln

50 55 60

Thr Trp Phe Asp Arg Lys Gln Lys Lys Ile Arg Asn Trp Ala Asp Arg

65 70 75 80

Thr Ser Gly Lys Ile Asp Asn Trp Phe Gly Glu Val Asp Pro Gln Lys

85 90 95

Pro Ala Ser Ala Thr Ile Arg Val Met Ile Asp Asn Tyr Trp Asn Glu

100 105 110

Tyr Asp Asn Tyr Glu Ile Lys Pro Arg Ile Arg Gly Lys Ile Lys Leu

115 120 125

Pro Thr Leu Glu Lys Arg Leu Ser Val Val Phe Gly Asp Asp Ser Leu

130 135 140

Asp Asp Glu Phe Asn Asn Ser Pro Ala Asn Ile Asn Gln Asn Pro Asn

145 150 155 160

Gln Asp Pro Asn Lys Lys Leu Asp Gly Lys Arg Thr Arg Asp Asp Asn

165 170 175

Ser Ser Ile Ala Leu Arg Trp Ser Asn Phe Ser Lys Lys Leu Pro Phe

180 185 190

Glu Thr Asp Ala Asp Leu Gly Ile Arg Ser Gly Asp Asp Ile Tyr Val

195 200 205

Arg Leu Lys Ala Ser Arg Asp Trp Gln Leu Arg Asn Asp Phe Lys Phe

210 215 220

Tyr Ala Glu Gln Ile Tyr Arg Tyr Gly Ile Asp Ser Glu Asn Tyr Leu

225 230 235 240

Arg Thr Asn Leu Glu Leu Thr His Ala Arg Pro Asn Gln Pro Ile Leu

245 250 255

Ser Asn Gln Phe Ser Leu Thr Tyr Ala Asp Asp Gln Asp Asp Asp Leu

260 265 270

Thr Trp Glu Asn Arg Leu Phe Arg Glu His Ser Phe Phe Ala Asn Asn

275 280 285

Arg Phe Asn Tyr Gly Ile Tyr Thr Gly Gly Tyr Tyr Asn Asp Asn Asp

290 295 300

Leu Arg Leu Asn Ser Trp Gly Pro Phe Val Ser Trp Arg Gln Pro Val

305 310 315 320

Leu Arg Glu Trp Phe Phe Val Gln Gly Asp Leu Asn Tyr Phe Asn Asp

325 330 335

His Arg Glu Asp Arg Asn His Tyr Val Ser Thr Phe Leu Arg Leu Glu

340 345 350

Ala Leu Phe

355

<210> 56

<211> 108

<212> PRT

<213> Acinetobacter baumannii

<400> 56

Met Ser Thr Leu Leu Val Ala Cys Asn Lys His Glu Asn Lys Thr Glu

1 5 10 15

Thr Thr Ser Asp Ala Ser Thr Pro Val Gln Thr Ala Gln Ser Asn Asn

20 25 30

Asn Glu Ala Val Asp Thr Ala His Thr Ala Glu Asn Ser Leu Asp Trp

35 40 45

Asp Gly Lys Tyr Lys Gly Thr Leu Pro Cys Ala Asp Cys Glu Gly Ile

50 55 60

Lys Thr Glu Leu Glu Leu Lys Asp Asp Lys Thr Tyr Glu Leu Thr Glu

65 70 75 80

Thr Tyr Leu Gly Lys Gly Asp Ala Asn Pro Phe Glu Pro Met Val Ser

85 90 95

Leu Leu Ser Ile Lys Thr Ile Leu Leu Leu Leu Pro

100 105

<210> 57

<211> 635

<212> PRT

<213> Acinetobacter baumannii

<400> 57

Met Arg Gly Phe Gly Ala Arg Ser Thr Phe Gly Val Arg Gly Ile Arg

1 5 10 15

Leu Tyr Val Asp Gly Ile Pro Ala Thr Met Pro Asp Gly Gln Gly Gln

20 25 30

Thr Ser Asn Ile Asp Leu Ser Ser Leu Asp His Val Glu Val Leu Thr

35 40 45

Gly Pro Phe Ser Ser Leu Tyr Gly Asn Ser Ser Gly Gly Thr Ile Leu

50 55 60

Thr Ser Thr Lys Glu Gly Gln Gly Lys Asp Ser Ile Glu Leu Ser Tyr

65 70 75 80

Ser Gly Gly Ser His Asp Lys Ser Arg Ala Gly Leu Val Leu Gln Gly

85 90 95

Gly Ala Lys Gly Ala Asn Glu Pro Ser Tyr Ile Ile Ser Ser Ser Tyr

100 105 110

Phe Asp Thr Asp Gly Tyr Arg Glu His Ser Gly Ala Glu Lys Val Leu

115 120 125

Asn Asn Ala Lys Leu Ser Trp Asn Leu Asp Asp Gly Ser Lys Ile Asn

130 135 140

Trp Val Thr Asn Tyr Val Lys Ile His Ala Asp Asp Pro Met Gly Leu

145 150 155 160

Glu Arg Lys Asp Trp Gln Ala Asn Pro Lys Gln Ile Ala Pro Tyr Val

165 170 175

Lys Lys Trp Gly Phe Asn Ala Arg Lys Asp Ile Glu Gln Thr Gln Thr

180 185 190

Gly Ile Thr Trp Phe Lys Pro Ile Asn Asp Gln His Glu Leu Tyr Ala

195 200 205

Met Ala Tyr Leu Gly Asn Arg Gln Val Thr Gln Tyr Gln Ser Ile Pro

210 215 220

Gln Gly Gln Val Val Val Glu Asn Gly Lys Pro Val Tyr Thr Gly Gln

225 230 235 240

Lys Ser Pro Lys His Ala Gly Gly Val Ile Asp Phe Glu Arg Asn Tyr

245 250 255

Tyr Gly Ala Asp Phe Arg Trp Thr Gly Lys Glu Leu Leu Pro Asn Thr

260 265 270

Thr Val Ser Ile Gly Val Ala Phe Asp Ala Met Asp Glu Glu Arg Lys

275 280 285

Gly Phe Glu Asn Phe Asn Ala Asp Gly Ile Tyr Gly Val Lys Gly Asn

290 295 300

Leu Arg Arg Asp Glu Asp Asn Thr Leu Trp Asn Ile Asp Pro Tyr Leu

305 310 315 320

Gln Ala Ser Trp Gln Phe Leu Pro Thr Trp Arg Leu Asp Thr Gly Val

325 330 335

Arg Tyr Ser Asn Val His Tyr Lys Ser Lys Asp His Phe Thr Ser Gly

340 345 350

Pro Asp Glu Tyr Gly Thr Val Asn Gly Asp Asp Ser Gly Lys Thr Asp

355 360 365

Tyr Glu Lys Val Leu Pro Ser Ala Ala Leu Ser Trp Gln Ile Leu Pro

370 375 380

Glu Leu Met Ala Tyr Val Ser Tyr Ala Lys Gly Phe Glu Thr Pro Thr

385 390 395 400

Phe Thr Glu Met Ala Tyr His Thr Asp Ile Ser Lys Ser Gly Phe Asn

405 410 415

Phe Gly Leu Lys Pro Ser Thr Ser Asp Thr Tyr Glu Thr Gly Leu Lys

420 425 430

Ser Gln Asn Leu Leu Gly Asp Phe Thr Leu Ala Val Phe Gln Thr Lys

435 440 445

Thr Lys Asn Asp Ile Val Ser Ala Gly Asn Leu Gly Gly Arg Ser Thr

450 455 460

Phe Arg Asn Ala Asp Lys Thr Leu Arg Glu Gly Val Glu Phe Ala Trp

465 470 475 480

Asn Lys Lys Leu Trp Arg Asp Leu Thr Ala Thr Ala Ser Tyr Thr Tyr

485 490 495

Leu Asp Ala Thr Phe Asp Ala Asn Val Pro Glu Lys Leu Asp Gln Asp

500 505 510

Asn Lys Val Leu Ala Ser Ala Ile Pro Thr Gly Asn Ala Ile Pro Gly

515 520 525

Ile Ala Lys Asn Gln Ala Tyr Ala Ser Leu Ala Trp Gln Pro Ser His

530 535 540

Gly Leu Tyr Gly Gly Val Asp Val Gln Tyr Met Asp Lys Val Tyr Val

545 550 555 560

Asn Asp Thr Asn Ser Asp Ala Ala Pro Ser Tyr Ser Val Thr Ser Ala

565 570 575

Asn Val Gly Tyr Ala Trp Val Met Gly Asp Trp Lys Val Asn Ser Phe

580 585 590

Ala Arg Val Asp Asn Leu Phe Asp Lys Lys Tyr Ala Gly Ser Val Ile

595 600 605

Val Asn Asp Gly Asn Ser Arg Tyr Phe Glu Pro Ala Asp Gly Arg Asn

610 615 620

Trp Ser Ala Gly Leu Arg Val Ile Lys Gln Phe

625 630 635

<210> 58

<211> 381

<212> PRT

<213> Acinetobacter baumannii

<400> 58

Met Thr Gly Ile Ser Ser Val Tyr Ala Gln Glu Gln Val Asp Pro Ala

1 5 10 15

Tyr Lys Pro Gly Asn Pro Ile Tyr Asp Lys Trp Asp Arg Phe Tyr Lys

20 25 30

Ile Glu Gln Ser Gln Pro Gln Glu Ala Glu Lys Ile Leu Val Glu Leu

35 40 45

Ser Lys Leu Thr Pro Thr Asp Ile Lys Val Trp Lys Ser Leu Thr Tyr

50 55 60

Leu Gln Ile Arg Leu Glu Lys Arg Glu Glu Ala Leu Gln Ser Leu Arg

65 70 75 80

Gln Ala Arg Asn Leu Ala Pro Gln Asp Asp Thr Leu Lys Leu Gln Glu

85 90 95

Ala Tyr Leu Leu Asn Gln Gln Lys Lys Asp Arg Glu Ala Leu Val Leu

100 105 110

Phe Lys Glu Leu Ser Ser Ser Ser Asp Pro Glu Ile Ala Ala Lys Ala

115 120 125

Thr Gln Ala Val Lys Asn Leu Ser Gly Gly Glu Val Lys Pro Tyr Phe

130 135 140

Lys Asp Ile Tyr Phe Ala Pro Ser Tyr Glu Ser Arg Tyr Asp Asp Val

145 150 155 160

Ile Phe Pro Leu Lys Met Arg Tyr Gly Lys Asn Ile Asp Asn Gly Arg

165 170 175

Ala Gln Val Tyr Ala Phe Leu Asn Leu Asn Arg Asp Thr Gln Ser Gln

180 185 190

Gly Gly Val Arg Pro Glu Ile Ile Asp Glu Asn Ala Ala Thr Leu Gly

195 200 205

Leu Gly Ala Asn Tyr Gln Pro Trp Thr Ser Ile Pro Val Arg Ala Tyr

210 215 220

Val Glu Val Gly Gly Ser Tyr Asp Leu Ile Asp Arg Asn Arg Lys Arg

225 230 235 240

Phe Arg Glu Ser Val Val Gly Gly Val Thr Gly Tyr Gln Glu Trp Tyr

245 250 255

Ser Gln Ser Asn Cys Asp His Ser Leu Cys Leu Asp Asn Tyr Phe Thr

260 265 270

Asp Leu Tyr Gly Asn Val Ala Thr Tyr Ser Arg Glu Asp Tyr Asn Val

275 280 285

Ile Gly Asp Leu Arg Leu Arg Thr Gly Leu Asn Leu Tyr Lys Gly Glu

290 295 300

Ser Gly Thr Val Gln Ala Tyr Val Lys Leu His Gly Leu Ala Asp Ser

305 310 315 320

Glu Asp Glu Tyr Tyr Asn Asn Leu Phe Glu Tyr Gly Pro Gly Ile Ser

325 330 335

Trp Gln Pro Phe Asn Tyr Gln Pro Ile Lys Leu Arg Val Glu Arg Leu

340 345 350

Tyr Gly Asn Tyr Phe Lys Asp Val Pro Val Asn Thr Lys Asp His Tyr

355 360 365

Asn Asn Thr Arg Val Glu Leu Val Phe Tyr Lys Asp Phe

370 375 380

<210> 59

<211> 212

<212> PRT

<213> Acinetobacter baumannii

<400> 59

Met Ala Ile Gly Leu Val Gly Arg Lys Cys Gly Met Thr Arg Ile Phe

1 5 10 15

Thr Asp Ala Gly Val Ser Val Pro Val Thr Val Ile Glu Val Asp Pro

20 25 30

Asn Arg Ile Thr Gln Ile Lys Thr Leu Glu Thr Asp Gly Tyr Gln Ala

35 40 45

Val Gln Val Thr Thr Gly Glu Arg Arg Glu Ser Arg Val Thr Asn Ala

50 55 60

Gln Lys Gly His Phe Ala Lys Ala Gly Val Ala Ala Gly Arg Leu Val

65 70 75 80

Lys Glu Phe Arg Val Thr Glu Ala Glu Leu Glu Gly Arg Glu Ile Gly

85 90 95

Gly Thr Ile Gly Val Asp Leu Phe Thr Val Gly Gln Val Val Asp Val

100 105 110

Thr Gly Gln Ser Lys Gly Lys Gly Phe Gln Gly Gly Val Lys Arg Trp

115 120 125

Asn Phe Arg Thr Gln Asp Ala Thr His Gly Asn Ser Val Ser His Arg

130 135 140

Val Leu Gly Ser Thr Gly Gln Asn Gln Thr Pro Gly Arg Val Phe Lys

145 150 155 160

Gly Lys Lys Met Ala Gly His Leu Gly Asp Glu Arg Val Thr Val Gln

165 170 175

Gly Leu Glu Ile Val Ser Ile Asp Ala Glu Arg Ser Val Leu Val Val

180 185 190

Lys Gly Ala Ile Pro Gly Ala Thr Gly Gly Asp Val Ile Val Arg Pro

195 200 205

Thr Ile Lys Ala

210

<210> 60

<211> 424

<212> PRT

<213> Acinetobacter baumannii

<400> 60

Met Leu Lys Ala Gln Lys Leu Thr Leu Ala Val Leu Ile Ser Ala Ala

1 5 10 15

Ile Ile Ser Ser Ala Gln Ala Ser Glu Gln Ser Glu Ala Lys Gly Phe

20 25 30

Val Glu Asp Ala Asn Gly Ser Ile Leu Phe Arg Thr Gly Tyr Ile Ser

35 40 45

Arg Asp Lys Lys Asp Gly Arg Ala Asp Asn Ser Ser Phe Ala Gln Thr

50 55 60

Ala Ile Val Asn Ile Asp Ser Gly Phe Thr Pro Gly Ile Val Gly Phe

65 70 75 80

Gly Val Gly Val Val Gly Asp Gly Ser Phe Lys Ile Gly Glu Asn Lys

85 90 95

Asn Ala Gly Asn Asn Met Ile Pro Gln His Asn Asp Gly Ser Ala Tyr

100 105 110

Asp His Trp Ala Arg Gly Gly Ala Asn Val Lys Ala Arg Phe Ser Asn

115 120 125

Thr Thr Val Arg Tyr Gly Thr Gln Val Leu Asp Leu Pro Val Leu Ala

130 135 140

Ser Asn Thr Ala Arg Leu Val Pro Glu Tyr Phe Thr Gly Thr Leu Leu

145 150 155 160

Thr Ser His Glu Ile Lys Asp Leu Glu Val Val Ala Gly Lys Phe Thr

165 170 175

Lys Asn Gln Tyr Ser Asp Gln Ile Ala Thr Asp Gln Asn Gly Leu Asp

180 185 190

Arg Ala Val Val Trp Gly Ala Lys Tyr Lys Phe Asp Asp Gln Ile Ser

195 200 205

Gly Ser Tyr Tyr Gly Val Asp Val Lys Asp Lys Leu Asp Arg His Tyr

210 215 220

Val Asn Val Asn Tyr Lys Gln Pro Leu Ala Asn Asp Ser Ser Leu Thr

225 230 235 240

Tyr Asp Phe Ser Gly Tyr His Thr Lys Phe Asp Lys Gly Ala Asn Leu

245 250 255

Ser Tyr Ala Thr Gly Pro Ala Asp Glu Asp Lys Thr Asn Asn Ile Trp

260 265 270

Ala Ile Ser Gly Thr Tyr Ala Thr Gly Pro His Ser Val Met Leu Ala

275 280 285

Tyr Gln Gln Asn Ser Gly Asn Ile Gly Tyr Asn Tyr Gly Val Asn Gln

290 295 300

Asp Gly Gly Gln Ser Val Tyr Leu Pro Asn Ser Tyr Leu Ser Asp Phe

305 310 315 320

Ile Gly Asn Asp Glu Lys Ser Ala Gln Ile Gln Tyr Ser Leu Asp Phe

325 330 335

Gly Lys Leu Gly Val Leu Pro Gly Leu Asn Trp Thr Thr Ala Tyr Val

340 345 350

Tyr Gly Trp Asp Ile Lys Thr Ser Asn Gly Ala Asp Asp Ser Asn Glu

355 360 365

Ser Glu Phe Phe Asn Gln Val Lys Tyr Thr Val Gln Ser Gly Phe Ala

370 375 380

Lys Gly Ser Ser Leu Arg Leu Arg Asn Ser Ile Tyr Arg Ala Asp Asn

385 390 395 400

Ala Tyr Thr Thr Asp Tyr Met Pro Asp Thr Asn Glu Trp Arg Ile Phe

405 410 415

Leu Asp Ile Pro Val Thr Leu Phe

420

<210> 61

<211> 641

<212> PRT

<213> Acinetobacter baumannii

<400> 61

Met Gln Leu Lys Asp Val Pro Gln Ile Val Asn Val Val Pro Lys Gln

1 5 10 15

Val Leu Arg Glu Gln Thr Val Thr Ser Met Gln Gly Ala Leu Gln Asn

20 25 30

Val Ala Gly Leu Ser Phe Ser Val Gly Asp Gly Gln Arg Asp Gln Val

35 40 45

Met Ile Arg Gly Phe Ser Ala Ile Thr Asp Asn Tyr Val Asp Gly Ile

50 55 60

Arg Asp Asp Ala Leu Tyr Phe Arg Asp Met Ser Asn Val Glu Arg Ile

65 70 75 80

Glu Val Leu Lys Gly Pro Ala Ser Val Leu Tyr Gly Arg Gly Ser Ala

85 90 95

Gly Gly Leu Val Asn Lys Ile Asn Lys Lys Pro Met Asp Gln Ser Leu

100 105 110

Arg Glu Val Ser Leu Ile Gly Ser Thr Thr Gly Gln Arg Arg Ala Glu

115 120 125

Val Asp Val Asn Glu Lys Val Ala Glu Asn Val Lys Val Arg Leu Thr

130 135 140

Gly Ala Val Glu Asp Ser Asp Gly Tyr Arg Asp Gln Ala Phe Leu Lys

145 150 155 160

Arg Gln Ala Val Ala Pro Ser Val Gln Trp Asp Ile Thr Asp Lys Thr

165 170 175

Lys Leu Leu Leu Gln Ala Asp Tyr Leu His Asp Asn Arg Leu Ala Asp

180 185 190

Gln Gly Phe Pro Thr Asp Pro Ile Thr Gly Lys Pro Val Lys Thr Asn

195 200 205

Pro Lys Thr Phe Tyr Gly Ala Leu Asn Gly Lys Glu Val Gly Asp Val

210 215 220

Asp Thr Glu Ile Ser Ser Gln Thr Ile Ser Leu Asp His Glu Phe Asn

225 230 235 240

Asp Asn Phe Lys Tyr His Gly Ala Val Arg His Tyr Asn Tyr Ser Leu

245 250 255

Asp Arg Gln Tyr Ser Val Asp Ser His Gln Lys Leu Pro Ala Asp Gln

260 265 270

Ile Gln Leu Thr Gln Asn Lys Arg Leu Arg Asn Glu Asp Gly Val Tyr

275 280 285

Val Gln Gln Glu Leu Ser Ala Val Phe Asn Thr Gly Phe Leu Lys His

290 295 300

Ser Thr Leu Ile Gly Ala Glu Tyr Ser Lys Gln His Lys Asp Glu Leu

305 310 315 320

Val Trp Ser Lys Ala Arg Gln Ile Thr Asn Ile Phe Asn Pro Gln Leu

325 330 335

Glu Asn Trp Ala Pro Leu Asp Thr Asn Val Asp Ala Asp Thr Asn Asn

340 345 350

Thr Asn Thr Phe Glu Asn Tyr Gly Val Tyr Leu Gln Asp Leu Met Thr

355 360 365

Val Thr Asp Gln Leu Lys Val Leu Val Gly Leu Arg Tyr Asp Asn Leu

370 375 380

Ser Gln Asp Arg Asp Asp Lys Thr Ser Lys Asn Val Asp Leu Asn Arg

385 390 395 400

Thr Asp Asn Thr Tyr Ser Pro Arg Ile Gly Val Val Tyr Gln Pro Val

405 410 415

Asn Asn Leu Ser Leu Tyr Thr Ser Tyr Asn Arg Ser Phe Gln Pro Leu

420 425 430

Ala Asp Ser Phe Val Phe Tyr Lys Asn Ser Asp Asp Leu Arg Pro Thr

435 440 445

Lys Thr Glu Asn Tyr Glu Ile Gly Ala Lys Trp Asp Val Asn Asp Gln

450 455 460

Leu Asn Val Thr Leu Ala Leu Phe Glu Met Ser Gln Thr Asn Ile Gln

465 470 475 480

Asn Lys Asp Pro Asn Asp Pro Lys Gly Leu Thr Ala Ile Leu Ala Gly

485 490 495

Glu Gln Lys Thr Lys Gly Val Glu Ile Ser Leu Ala Gly Gln Leu Thr

500 505 510

Asp Gln Leu Ser Val Leu Ala Gly Tyr Ser Tyr Met Asp Gly Lys Ile

515 520 525

Glu Lys Ser Ala Ile Gly Phe Thr Gly Asn His Ser Ala Leu Thr Pro

530 535 540

Asn Asn Thr Ala Asn Leu Trp Leu Lys Tyr Gln Ile Asn Asp His Trp

545 550 555 560

Tyr Ala Ala Val Gly Gly Arg Gly Glu Ser Ser Arg Phe Ser Ala Pro

565 570 575

Asp Asn Lys Asn Val Leu Pro Gly Tyr Ala Val Val Asn Ala Ala Leu

580 585 590

Gly Tyr Gln Ser Glu Arg Tyr Asp Val Asn Leu Asn Leu Asn Asn Leu

595 600 605

Phe Asp Arg Asp Tyr Phe Val Ser Gly His Ser Gly Ala Asn Asp Ser

610 615 620

Asn Met Met Gly Asp Pro Leu Asn Ala Gln Val Ala Leu Arg Tyr Arg

625 630 635 640

Phe

<210> 62

<211> 209

<212> PRT

<213> Acinetobacter baumannii

<400> 62

Met Glu Glu Ala Val Gln Val Leu Asn Ser Leu Pro Ala Ala Lys Phe

1 5 10 15

Lys Glu Ser Leu Asp Ile Ser Val Asn Leu Gly Val Asp Pro Arg Lys

20 25 30

Ser Asp Gln Val Val Arg Gly Ala Thr Thr Leu Pro Ala Gly Thr Gly

35 40 45

Lys Thr Val Arg Val Ala Val Phe Ala Gln Gly Ala Gln Ala Glu Ala

50 55 60

Ala Lys Glu Ala Gly Ala Asp Val Val Gly Phe Asp Asp Leu Ala Glu

65 70 75 80

Ser Ile Gln Gly Gly Asn Leu Asp Phe Asp Val Val Ile Ala Ala Pro

85 90 95

Asp Ala Met Arg Val Val Gly Lys Leu Gly Thr Ile Leu Gly Pro Arg

100 105 110

Gly Leu Met Pro Asn Pro Lys Val Gly Thr Val Thr Pro Asp Val Ala

115 120 125

Gly Ala Val Lys Asn Ala Lys Ser Gly Gln Ala Arg Tyr Arg Val Asp

130 135 140

Lys Ala Gly Ile Ile His Ala Ala Ile Gly Gln Val Gly Phe Asp Ala

145 150 155 160

Ala Ala Ile Arg Gln Asn Val Glu Thr Leu Val Ala Asp Leu Lys Lys

165 170 175

Leu Lys Pro Ala Thr Ser Lys Gly Val Tyr Ile Lys Lys Ile Thr Leu

180 185 190

Ser Ser Thr Met Gly Pro Gly Leu Thr Val Asp Val Asn Asn Val Ser

195 200 205

Asn

<210> 63

<211> 147

<212> PRT

<213> Acinetobacter baumannii

<400> 63

Met Tyr Pro Ala Pro Thr Val Asp Gln Leu Ala Ile Asp His Ala Pro

1 5 10 15

Lys Phe Glu Asn Lys Arg Gly Asn Arg Phe Ala Leu Pro Arg Pro Glu

20 25 30

Pro Leu Gln Thr Asp Thr Thr Ala Asp Ala Ser Ala Gln Thr Gly Ser

35 40 45

Ala Leu Gly Arg Pro Gln Leu Val Thr Asp Gly Asn Lys Asn Pro Leu

50 55 60

Leu Lys Ile Asp Gly Ser Thr Ala Glu Ile Trp Gln Tyr Thr Lys Ala

65 70 75 80

Thr Leu Ser Thr Leu Asn Tyr Asn Val Ile Ala Gln Gly Asn Asn Gln

85 90 95

Ala Thr Ile Lys Val Asn Asp Asn Thr Tyr Val Leu Lys Leu Thr Gly

100 105 110

Val Gly Ser Ser His Ser Leu Ala Leu Phe Asn Pro Asp Asn Thr Phe

115 120 125

Ala Ser Pro Asp Val Ala Ala Glu Val Leu Asn Gln Ile Tyr Gln Asn

130 135 140

Trp Pro Ala

145

<210> 64

<211> 201

<212> PRT

<213> Acinetobacter baumannii

<400> 64

Met Lys Val Ser Gly Leu Val Gly Thr Asn Leu Ser Asp Gly Tyr Thr

1 5 10 15

Met Lys Ala Gln Phe Asp Asn Val Asn Gly Leu Lys Pro Arg Ala Lys

20 25 30

Val Thr Met Ser Gly Val Thr Ile Gly Arg Val Asp Ser Ile Thr Leu

35 40 45

Asp Pro Val Thr Arg Leu Ala Thr Val Thr Phe Asp Leu Asp Gly Lys

50 55 60

Leu Thr Ser Phe Asn Ala Glu Gln Leu Lys Glu Val Gln Lys Asn Ala

65 70 75 80

Leu Asp Glu Leu Arg Tyr Ser Ser Asp Tyr Thr Gln Ala Thr Pro Ala

85 90 95

Gln Gln Lys Thr Met Glu Gln Gln Leu Ile Ser Asn Met Asn Ser Ile

100 105 110

Thr Ser Ile Asp Glu Asp Ala Tyr Ile Met Val Ala Thr Asn Gly Leu

115 120 125

Leu Gly Glu Lys Tyr Leu Lys Ile Val Pro Gly Gly Gly Leu Asn Tyr

130 135 140

Leu Lys Arg Gly Asp Thr Ile Ser Asn Thr Gln Gly Thr Met Asp Leu

145 150 155 160

Glu Asp Leu Ile Ser Lys Phe Ile Thr Gly Gly Gly Ala Gly Lys Val

165 170 175

Ala Ala Gly Ser Ser Ser Ala Glu Glu Lys Ala Pro Ala Ser Thr Asp

180 185 190

Ser Ser Ala Gln Pro Ser Phe Val Glu

195 200

<210> 65

<211> 183

<212> PRT

<213> Acinetobacter baumannii

<400> 65

Met Gly Val Ser Ser Phe Thr Phe Ala Gly Asn Trp Gln Val Lys Phe

1 5 10 15

Gly Gly Ser Val Ile Ala Pro Ser Glu Asp Thr Thr Thr Ala Leu Gly

20 25 30

Val Val Lys Ala Asp His Glu Tyr Ala Phe Thr Pro Ser Val Glu Tyr

35 40 45

Phe Phe Gly Gln Ser Pro Phe Ser Ala Glu Leu Leu Leu Ala Thr Pro

50 55 60

Val Asn His Asp Val Leu Leu Asp Gly Gln Lys Val Ala Arg Ile Lys

65 70 75 80

Gln Leu Pro Pro Thr Ile Thr Ala Lys Tyr His Phe Lys Asn Ser Thr

85 90 95

Arg Phe Thr Pro Tyr Ile Gly Ile Gly Ala Thr Ala Phe Ile Pro Trp

100 105 110

Asp Glu Gln Gly Val Ala Asp Lys Val Lys Glu Asp Phe Gly Val Ala

115 120 125

Gly Gln Ile Gly Phe Asn Phe Gln Pro Ala Asp Ala Lys Asn Trp Gly

130 135 140

Val Phe Val Asp Val Arg Tyr Ala Asp Ile Ser Pro Glu Val Thr Leu

145 150 155 160

Thr Asn Gly Ala Lys Phe Asp Leu Asp Ile Asn Pro Phe Val Tyr Thr

165 170 175

Leu Gly Tyr Ser Tyr Lys Phe

180

<210> 66

<211> 231

<212> PRT

<213> Acinetobacter baumannii

<400> 66

Met Met Lys Ile Leu Lys Leu Ser Phe Leu Ala Leu Gly Met Gly Leu

1 5 10 15

Ser Gly Phe Ala Gln Ala Asp Phe Ile Gly Val Lys Gly Asp Val Gly

20 25 30

Tyr Trp Phe Tyr Asp Gly Lys Ala Asn Met Ser Ser Gln Ser Pro Glu

35 40 45

Asp Gln Asp Leu Asp Arg Lys Gly Ser Ala Gln Leu Ser Leu Ala Phe

50 55 60

Glu His Pro Ile Pro Phe Ile Pro Asn Ala Lys Ile Arg Tyr Val Asn

65 70 75 80

Leu Asp Thr Gln Thr Lys Ser Glu Thr Leu Gly Gln Ala Asn Tyr Asn

85 90 95

Val Asp Leu Asp His Ser Asp Phe Ile Leu Tyr Tyr Glu Leu Leu Asp

100 105 110

Asn Ile Val Ser Val Asp Ala Gly Leu Gly Ala Thr Val Leu Asn Gly

115 120 125

Asp Ile Thr Ala Tyr Thr Gly Lys Arg Val Asp Ile Asp Lys Thr Tyr

130 135 140

Pro Ile Ala Tyr Leu Ser Gly Glu Val Lys Leu Pro Phe Thr Gly Leu

145 150 155 160

Ser Ala Lys Gly Glu Ala Thr Tyr Thr Asn Phe Asp Asp Ala Lys Ile

165 170 175

Thr Asp Ala Leu Val Glu Ala Lys Tyr Lys Phe Ala Asp Asn Leu Leu

180 185 190

Ile Asp Leu Gly Leu Thr Ala Gly Tyr Arg Ile Leu Asn Ile Asp Leu

195 200 205

Asp Asp Tyr Asp Asn Asn Asp Leu Lys Phe Glu Phe Lys Gly Pro Tyr

210 215 220

Val Gly Leu Glu Ala His Phe

225 230

<210> 67

<211> 142

<212> PRT

<213> Acinetobacter baumannii

<400> 67

Met Asp Asp Leu Lys Glu Asn Val Lys Glu Lys Gln Thr Ala Gly Lys

1 5 10 15

Glu Ala Val Ala Asp Lys Val Asp Glu Leu Lys Thr Lys Ala Ala Asp

20 25 30

Ala Lys Val Gln Gly Glu Lys Ala Leu Glu Asp Leu Lys Glu Asn Val

35 40 45

Lys Glu Lys Gln Ala Ala Ala Lys Glu Ala Val Glu Asp Lys Ala Ser

50 55 60

Asp Leu Lys Gly Lys Leu Asp Asp Ala Gln His Ser Leu Gln Asp Lys

65 70 75 80

Phe Asp His Leu Arg Thr Glu Ala Ala His Lys Leu Asp Asp Ala Lys

85 90 95

Ala Lys Ala Ala Glu Leu Lys Glu Glu Ala Ala Thr Lys Phe Asp Glu

100 105 110

Leu Lys Thr Gln Ala Thr Ala Lys Phe Asp Glu Leu Lys Lys Thr Ala

115 120 125

Thr Glu Lys Leu Asn Lys Leu Lys Asn His Asp Ser Ala Glu

130 135 140

<210> 68

<211> 344

<212> PRT

<213> Acinetobacter baumannii

<400> 68

Met Lys Ala Gln Val His Ala Gly Gly Arg Gly Lys Ala Gly Gly Val

1 5 10 15

Lys Val Ala Lys Ser Lys Glu Asp Val Ile Glu Phe Ala Asn Asn Ile

20 25 30

Ile Gly Thr Arg Leu Val Thr Tyr Gln Thr Asp Ala Asn Gly Gln Pro

35 40 45

Val Asn Ser Ile Ile Val Ala Glu Asp Val Tyr Pro Val Glu Arg Glu

50 55 60

Leu Tyr Leu Gly Ala Val Val Asp Arg Ser Ser Arg Arg Ile Thr Phe

65 70 75 80

Met Ala Ser Thr Glu Gly Gly Val Glu Ile Glu Lys Val Ala Glu Glu

85 90 95

Thr Pro Glu Lys Ile Ile Lys Val Glu Val Asp Pro Leu Val Gly Leu

100 105 110

Gln Pro Phe Gln Ala Arg Glu Val Ala Phe Ala Leu Gly Leu Lys Asp

115 120 125

Lys Gln Ile Gly Gln Phe Val Lys Ile Met Thr Ala Ala Tyr Gln Ala

130 135 140

Phe Val Glu Asn Asp Phe Ala Leu Phe Glu Ile Asn Pro Leu Ser Val

145 150 155 160

Arg Glu Asn Gly Glu Ile Leu Cys Val Asp Ala Lys Val Gly Ile Asp

165 170 175

Ser Asn Ala Leu Tyr Arg Leu Pro Lys Val Ala Ala Leu Arg Asp Lys

180 185 190

Ser Gln Glu Asn Glu Arg Glu Leu Lys Ala Ser Glu Phe Asp Leu Asn

195 200 205

Tyr Val Ala Leu Glu Gly Asn Ile Gly Cys Met Val Asn Gly Ala Gly

210 215 220

Leu Ala Met Ala Thr Met Asp Ile Ile Lys Leu Tyr Gly Gly Gln Pro

225 230 235 240

Ala Asn Phe Leu Asp Val Gly Gly Gly Ala Thr Lys Glu Arg Val Ile

245 250 255

Glu Ala Phe Lys Ile Ile Leu Ala Asp Thr Ser Val Gln Gly Val Leu

260 265 270

Ile Asn Ile Phe Gly Gly Ile Val Arg Cys Asp Met Ile Ala Glu Ala

275 280 285

Ile Ile Ala Ala Val Gln Glu Val Asn Val Thr Val Pro Val Val Val

290 295 300

Arg Leu Glu Gly Asn Asn Ala Glu Leu Gly Ala Lys Leu Leu Asp Glu

305 310 315 320

Ser Gly Leu Lys Leu Ile Ser Ala Asn Gly Leu Ser Asp Ala Ala Glu

325 330 335

Lys Val Val Ala Ala Val Lys Ala

340

<210> 69

<211> 415

<212> PRT

<213> Acinetobacter baumannii

<400> 69

Met Met Ser Ala Lys Leu Trp Ala Pro Ala Leu Thr Ala Cys Ala Leu

1 5 10 15

Ala Thr Ser Ile Ala Leu Val Gly Cys Ser Lys Gly Ser Asp Glu Lys

20 25 30

Gln Gln Ala Ala Ala Ala Gln Lys Met Pro Pro Ala Glu Val Gly Val

35 40 45

Ile Val Ala Gln Pro His Ser Val Glu Gln Ser Val Glu Leu Ser Gly

50 55 60

Arg Thr Ser Ala Tyr Gln Ile Ser Glu Val Arg Pro Gln Thr Ser Gly

65 70 75 80

Val Ile Leu Lys Arg Leu Phe Ala Glu Gly Ser Tyr Val Arg Glu Gly

85 90 95

Gln Ala Leu Tyr Glu Leu Asp Ser Arg Thr Asn Arg Ala Thr Leu Glu

100 105 110

Asn Ala Lys Ala Thr Leu Leu Gln Gln Gln Ala Asn Leu Ala Ser Leu

115 120 125

Arg Thr Lys Leu Asn Arg Tyr Lys Gln Leu Val Ser Ser Asn Ala Val

130 135 140

Ser Lys Gln Glu Tyr Asp Asp Leu Leu Gly Gln Val Asn Val Ala Glu

145 150 155 160

Ala Gln Val Ser Ala Ala Lys Ala Gln Val Thr Asn Ala Asn Val Asp

165 170 175

Leu Gly Tyr Ser Thr Ile Arg Ser Pro Ile Ser Gly Gln Ser Gly Arg

180 185 190

Ser Ser Val Thr Ala Gly Ala Leu Val Thr Ala Asn Gln Thr Asp Pro

195 200 205

Leu Val Thr Ile Gln Gln Leu Asp Pro Ile Tyr Val Asp Ile Asn Gln

210 215 220

Ser Ser Ala Glu Leu Leu Arg Leu Arg Gln Gln Leu Ser Lys Gly Ser

225 230 235 240

Leu Asn Asn Ser Asn Asn Thr Lys Val Lys Leu Lys Leu Glu Asp Gly

245 250 255

Ser Thr Tyr Pro Ile Glu Gly Gln Leu Ala Phe Ser Asp Ala Ser Ala

260 265 270

Asn Gln Asp Thr Gly Thr Ile Thr Leu Arg Ala Val Phe Ser Asn Pro

275 280 285

Asn His Leu Leu Leu Pro Gly Met Tyr Thr Thr Ala Gln Ile Val Gln

290 295 300

Gly Val Val Pro Asn Ala Tyr Leu Ile Pro Gln Ala Ala Ile Thr Arg

305 310 315 320

Leu Pro Thr Gly Gln Ala Val Ala Met Leu Val Asn Ala Lys Gly Ala

325 330 335

Val Glu Ser Arg Pro Val Glu Thr Ser Gly Val Gln Gly Gln Asn Trp

340 345 350

Ile Val Thr Asn Gly Leu Lys Ala Gly Asp Lys Val Ile Val Asp Gly

355 360 365

Val Ala Lys Val Lys Glu Gly Gln Glu Val Ser Ala Lys Pro Tyr Gln

370 375 380

Ala Gln Pro Ala Asn Pro Gln Gly Ala Ala Pro Asn Ala Thr Lys Pro

385 390 395 400

Ala Gln Ser Gly Lys Pro Gln Ala Glu Gln Lys Ala Ser Asn Ala

405 410 415

<210> 70

<211> 656

<212> PRT

<213> Acinetobacter baumannii

<400> 70

Met Asp Ser Ser Ser Ser Ala Thr Arg Ser Glu Ile Ala Leu Gln Asp

1 5 10 15

Thr Pro Gln Ser Val Ser Val Val Thr Gln Lys Val Ile Glu Asp Ile

20 25 30

Gly Ala Thr Arg Leu Val Glu Ala Leu Asp Leu Ala Gly Gly Val Thr

35 40 45

Arg Ala Asn Asn Phe Gly Gly Gln Gly Leu Thr Gly Phe Asn Val Arg

50 55 60

Gly Phe Thr Ser Gly Glu Phe Tyr Arg Asn Gly Phe Pro Ile Asn Arg

65 70 75 80

Gly Tyr Pro Asn Ala Pro Asp Ser Asn Thr Ile Glu Arg Val Asp Val

85 90 95

Leu Arg Gly Pro Ser Ser Ser Leu Tyr Gly Arg Gly Asp Pro Gly Gly

100 105 110

Thr Phe Asn Leu Ile Ser Lys Thr Pro Asn Ser Glu Gln Gln Thr Thr

115 120 125

Leu Gly Ala Gln Leu Asn Ser Glu Gly Leu Tyr Arg Thr Thr Val Asp

130 135 140

Thr Thr Gly Thr Ile Pro Asn Ala Glu Asn Ile Gly Tyr Arg Leu Asn

145 150 155 160

Val Ile Ala Glu Gly Gly Asp Ser Tyr Arg Asp His Val Glu Ser Lys

165 170 175

Arg Tyr Gly Ile Ala Pro Val Ile Gln Trp Gln Ala Thr Asp Ala Thr

180 185 190

Lys Val Thr Phe Glu Ala Asp Ile Leu Arg Asn Gln His Pro Leu Asp

195 200 205

Arg Gly His Thr Arg Tyr Pro Thr Gln Lys Ser Phe Asn Ser Ser Pro

210 215 220

Glu Thr Tyr Leu Trp Glu Thr Gly Lys Tyr Tyr Asn Arg Leu Tyr Asn

225 230 235 240

Asp Asn Asn Met Thr Gln Leu Arg Val Glu His Asp Leu Gly Asn Asp

245 250 255

Trp Lys Leu Asn Ala Gly Val Gln Tyr Leu Asn Gly Lys Leu His Gly

260 265 270

Tyr Ala Val Glu Ala Asn Gly Ile Gln Asn Asp Gly Glu Thr Leu Gly

275 280 285

Arg Asn Tyr Asn Tyr Arg Glu Leu Lys Trp Gln Asp Thr Asp Ala Gln

290 295 300

Ile Asn Leu Thr Gly Asn Phe Gln Leu Leu Gly Leu Ala His Thr Leu

305 310 315 320

Val Thr Gly Leu Glu Tyr Glu Asn Tyr Asp Tyr Lys Ser Tyr Ile Ile

325 330 335

Arg Ser Ser Glu Asp Ile Gly Ser Tyr Ser Ile Asn Ile Tyr Asn Pro

340 345 350

Val Leu Gly Gln Pro Leu Pro Glu Leu Asn Thr Val Thr Thr His Asp

355 360 365

Arg Glu Asn Leu Lys Thr Thr Ala Leu Phe Val Gln Asp Gln Leu Glu

370 375 380

Leu Asn Glu Arg Leu Ser Ala Leu Leu Gly Leu Arg Phe Glu His Tyr

385 390 395 400

Glu His Asp Tyr Gln Asp Leu Arg Pro Gly Lys Pro Asn Trp Asn Thr

405 410 415

Ser His Asp Ala Phe Ile Pro Arg Leu Gly Leu Val Tyr Lys Ala Ser

420 425 430

Asp Asp Leu Ser Leu Tyr Gly Asn Ala Ala Lys Ser Phe Lys Pro Asn

435 440 445

Thr Gly Ala Ser His Ser Gly Glu Gly Phe Asp Pro Glu Glu Gly Met

450 455 460

Ala Tyr Glu Leu Gly Phe Lys Trp Leu Ala Leu Asn Asn Met Leu Ser

465 470 475 480

Val Asp Ser Ala Ile Phe Tyr Ala Asn Lys Glu Asn Val Leu Thr Asn

485 490 495

Asp Pro Leu Phe Pro Asn Tyr Lys Val Ala Ala Gly Glu Val Arg Ser

500 505 510

Arg Gly Ile Glu Leu Asn Ile Ala Gly Gln Ile Thr Pro Ala Trp Lys

515 520 525

Ile Ile Gly Gly Tyr Ala Tyr Thr Asp Ala Glu Val Thr Lys Asp Asn

530 535 540

Thr Leu Gln Lys Gly Thr Ala Leu Ala Asn Ile Pro Lys Asn Ser Phe

545 550 555 560

Asn Leu Leu Asn Ile Tyr Glu Phe Gln Asp Gly Pro Leu Gln Gly Leu

565 570 575

Gly Leu Gly Ile Asn Gln Lys Tyr Ile Asp Lys Arg Ala Gly Gln Thr

580 585 590

Ala Asn Ser Thr Tyr Ile Met Lys Gly Tyr Ala Val Thr Asp Leu Val

595 600 605

Ser Tyr Tyr Gln Ala Thr Pro Lys Leu Arg Leu Asn Leu Asp Val Lys

610 615 620

Asn Ile Phe Asp Lys Val Tyr Asp Glu Ser Ala Phe Asn Leu Tyr Ala

625 630 635 640

Tyr Pro Gly Glu Ser Arg Thr Val Gln Leu Gly Met Ser Tyr Thr Phe

645 650 655

<210> 71

<211> 177

<212> PRT

<213> Acinetobacter baumannii

<400> 71

Met Asp Arg Met Arg Gly Glu Asp Asp Tyr Lys Ser Thr Tyr Phe Glu

1 5 10 15

Leu Ser Pro Arg Leu Ser Leu Gly Glu Val Ser Gly Lys Lys Leu Thr

20 25 30

Tyr Gly Pro Ile Lys Asp Val Leu Ile Ser Thr Thr Trp Glu Ser Asn

35 40 45

Thr Gln Asn Gly Asn Asn Phe Asp Asn Phe Leu Tyr Gly Phe Ala Val

50 55 60

Asp Leu Asp Ile Pro Tyr Phe Gln Tyr Ala Asn Leu Asn Phe Tyr Arg

65 70 75 80

Ala Asn Asn Glu Lys Thr Asp Asp Asp Tyr Gln Met Thr Phe Val Tyr

85 90 95

Gly Ile Pro Phe Lys Ile Ala Ser Glu Asp Phe Leu Val Asp Gly Phe

100 105 110

Leu Asp Trp Ser Thr Ala Glu Asp Asp His Ala Ser Glu Leu Asn Trp

115 120 125

Thr Thr Gln Trp Lys Trp Asn Val Gly Lys His Ile Ser Pro Asp Thr

130 135 140

Arg Leu Tyr Leu Gly Ile Glu His Ser Val Trp Asn Asn Lys Phe Gly

145 150 155 160

Ile Lys Gly Ala Asp Glu Asn Asn Val Ser Ala Leu Val Lys Tyr His

165 170 175

Phe

<210> 72

<211> 581

<212> PRT

<213> Acinetobacter baumannii

<400> 72

Met Tyr Ala Asn Lys Asn Gln Lys Ser Glu Val Thr Tyr Ala Gly Tyr

1 5 10 15

Phe Leu Ser Glu Asn Gly Asn Asn Asn Leu Lys Glu Glu Leu Lys Ala

20 25 30

Asp Ile Ser Ala Leu Phe Ile Pro Thr Gln Asp Asn Gln Ser Ile Arg

35 40 45

Cys Lys Phe Pro Ala Arg Ser Gln Trp Leu Ile Gln Gln Leu Gly Ile

50 55 60

Gln Glu Asn Glu Leu Pro Gln Val Lys Cys Ser Glu Phe Glu Asn Trp

65 70 75 80

Ile Gly Gln Ile Lys Pro Tyr Lys Ala Thr Leu Ile Tyr Ala Thr Asp

85 90 95

Phe Met Gly Asn Pro Ser Ser Met Phe Gly His Thr Leu Leu Arg Leu

100 105 110

Asp Pro Lys Asp Gln Gln Gln Leu Asn Leu Val Ser Tyr Ala Val Asn

115 120 125

Tyr Ala Ala Thr Val Ala Gly Asn Asp Asn Trp Ser Tyr Ala Trp Lys

130 135 140

Gly Leu Thr Gly Gln Tyr Pro Gly Glu Tyr Ser Leu Met Pro Tyr Tyr

145 150 155 160

Arg Lys Val Lys Glu Tyr Gly Asp Phe Glu Ser Arg Asp Leu Trp Glu

165 170 175

Tyr Glu Leu Asn Leu Ser Pro Glu Glu Thr Arg Phe Leu Val Ser His

180 185 190

Ile Trp Glu Met Gln His Val Ser Phe Pro Tyr Tyr Phe Val Ser Asp

195 200 205

Asn Cys Ala Tyr Arg Leu Leu Gly Leu Val Asp Leu Val Lys Pro Glu

210 215 220

Ser His Leu Gln Glu Lys Phe Asn Tyr Ala Ser Ile Pro Met Glu Thr

225 230 235 240

Ile Lys Ala Met Gln Gln Gln Gly Leu Thr Lys Ala Pro Val Tyr Arg

245 250 255

Pro Ala Leu Glu Thr Gln Leu Leu Ala Gln Ala His Gln His Gly Ala

260 265 270

Ser Leu Ala Lys Val Ala His Gln Leu Ala Met Lys Pro Ile Lys Glu

275 280 285

Ser Ser Glu Thr Leu Lys Ser Phe Ser Pro Ser Asp Gln Ala Lys Ile

290 295 300

Leu Glu Met Ala Tyr Asp Asp Leu Tyr Leu Gln Phe Ile Gly Arg Lys

305 310 315 320

Val Glu Glu Ser Phe Ala Gln Pro Gln Leu Arg Gln Leu Leu Ala Leu

325 330 335

Arg Ser Gln Ile Asp Leu Asp Lys Gln Arg Gln Glu Pro Lys Arg Pro

340 345 350

Ser Thr Glu Pro Thr Gln Gly His Asn Ala Arg Asn Val Ser Leu Lys

355 360 365

Leu Gly Glu Val Gln Gly Asp Lys Phe Ile Glu Ile Gly His Arg Gln

370 375 380

Ala Tyr His Asp Leu Ile Asp Pro Gln Gly Gly Tyr Arg Ala Gly Thr

385 390 395 400

Gln Leu Leu Phe Leu Asn Gly Asn Ala Gln Trp Arg Asp Asp His Leu

405 410 415

Lys Leu Glu Arg Leu Asp Leu Leu Glu Val Asn Ser Tyr Asn Pro Ile

420 425 430

Gln Pro Phe Lys Thr Pro Leu Thr Trp Gly Phe Asn Leu Gly Trp Arg

435 440 445

Gln Glu Ala Val His Asp Gly Val Tyr Ser Asp Glu Lys Gln His Gly

450 455 460

Val Ala Ser Phe Asn Ala Gln Val Gly Tyr Ser Leu Ala Asp Tyr Glu

465 470 475 480

Arg Lys His Ile Cys Tyr Gly Gln Val Gln Thr Tyr Val Gln Ala Gly

485 490 495

Ser Asn Leu Asp Lys Gly Trp Arg Val Gly Val Gly Pro Thr Leu Gly

500 505 510

Cys Met Asn Gln Trp Leu Glu Lys Phe Asn Thr Val Val Gln Val Glu

515 520 525

Leu Pro Tyr Trp Glu Asp Gln Asn Gln Trp Asn Leu Arg Leu Asn Thr

530 535 540

Gln Trp Gln Tyr Ala Ile Asn Ser Asn Asn Ala Ile Arg Phe Asn Trp

545 550 555 560

Asp Tyr Glu Lys Gln Asn His Leu Asp Trp Met Lys Ser Ser Leu Gly

565 570 575

Tyr Val Trp Phe Phe

580

<210> 73

<211> 205

<212> PRT

<213> Acinetobacter baumannii

<400> 73

Met Arg Gly Asn Asn Val Asn Leu Lys Thr Val Ser Gly Ser Ala Val

1 5 10 15

Glu Leu Ser Glu Val Ala Phe Gly Arg Glu Phe Asn Glu Ala Leu Val

20 25 30

His Gln Val Val Thr Ala Tyr Leu Ala Gly Gly Arg Gln Gly Thr Arg

35 40 45

Ala Gln Lys Ser Arg Ala Glu Val Ser Gly Gly Gly Lys Lys Pro Phe

50 55 60

Arg Gln Lys Gly Thr Gly Arg Ala Arg Ala Gly Ser Ile Arg Ser Pro

65 70 75 80

Ile Trp Val Gly Gly Gly Lys Thr Phe Ala Ala Arg Pro Gln Asp Trp

85 90 95

Ser Gln Lys Val Asn Arg Lys Met Tyr Arg Gly Ala Met Gln Cys Ile

100 105 110

Leu Ala Glu Leu Val Arg Gln Asp Arg Leu Val Leu Val Glu Glu Phe

115 120 125

Ala Val Ala Ala Pro Lys Thr Lys Glu Leu Leu Ala Lys Leu Asn Asp

130 135 140

Leu Asn Ala Ala Arg Ala Leu Ile Val Thr Asp Ala Val Asp Glu Asn

145 150 155 160

Leu Tyr Leu Ala Ala Arg Asn Leu Pro His Val Asp Val Val Asp Ala

165 170 175

Thr Ala Ile Asp Pro Val Ser Leu Ile Ala Phe Asp Lys Val Val Met

180 185 190

Ser Val Ala Ala Ala Lys Lys Ile Glu Val Glu Leu Gly

195 200 205

<210> 74

<211> 152

<212> PRT

<213> Acinetobacter baumannii

<400> 74

Met Lys Gly Ser Lys Gly Thr Leu Ser Phe Asn Leu His Ala Leu Val

1 5 10 15

Glu Leu Lys Gln Glu Glu Gly Lys Leu Gln Leu Ala Pro Ala Lys Glu

20 25 30

Ser Lys Asp Ala Trp Met Gln Ala Gly Thr Ala Arg Ala Val Leu Asn

35 40 45

Asn Leu Val Lys Gly Val Ser Glu Gly Phe Glu Arg Lys Leu Gln Leu

50 55 60

Val Gly Val Gly Tyr Lys Ala Ala Val Lys Gly Thr Val Val Asn Leu

65 70 75 80

Asn Leu Gly Tyr Ser His Pro Ile Asp Tyr Ala Leu Pro Glu Gly Val

85 90 95

Thr Ala Glu Thr Pro Thr Ala Thr Glu Ile Ile Leu Lys Ser Ala Asn

100 105 110

Lys Gln Leu Leu Gly Gln Val Ala Ala Glu Ile Arg Ala Tyr Arg Ser

115 120 125

Pro Glu Pro Tyr Lys Gly Lys Gly Val Arg Tyr Ser Asp Glu Val Ile

130 135 140

Leu Arg Lys Glu Ala Lys Lys Lys

145 150

<210> 75

<211> 221

<212> PRT

<213> Acinetobacter baumannii

<400> 75

Met Thr Asn Leu Thr Asp Tyr Val Val Leu Asn Thr Ser Val Ala Ser

1 5 10 15

Gly Glu Gln Asp Val Asn Ala Phe Gln Ser Phe Asn Tyr Leu Ala Ala

20 25 30

Tyr Asn Ala Ser Asn Lys Ala Lys Val Ala Ala Val Ala Thr Thr Tyr

35 40 45

Leu Glu Pro Met Gly Ile Tyr Ala Asn Lys Val Lys Thr Val Asp Glu

50 55 60

Phe Pro Gln Gly Ala Thr Ile Ala Ile Pro Asn Asp Thr Ala Asn Glu

65 70 75 80

Ala Arg Ala Leu Thr Leu Leu Gln Ser Ala Lys Leu Ile Lys Leu Lys

85 90 95

Pro Asp Phe Asp Pro Val Lys Gly Thr Thr Asn Asp Val Val Glu Asn

100 105 110

Pro Lys Asn Leu Gln Leu Lys Pro Ile Gln Met Thr Thr Ala Val Arg

115 120 125

Val Lys Asn Asp Val Asp Ala Ile Val Leu Gly Asn Thr Leu Ala Leu

130 135 140

Glu Gly Gly Leu Asn Val Met Lys Asp Ala Ile Phe Arg Glu Pro Ile

145 150 155 160

Asp Gln Ser Thr Lys Leu Tyr Val Asn Leu Leu Gly Val Ala Glu Ala

165 170 175

Asn Lys Asn Asp Pro Ile Tyr Thr Lys Leu Gly Glu Leu Tyr His Leu

180 185 190

Pro Lys Val Gln Lys Phe Val Asn Glu Lys Phe Gly Gly Thr Lys Val

195 200 205

Glu Val Asn Lys Pro Val Ser Glu Phe Ala Asp Ile Lys

210 215 220

<210> 76

<211> 99

<212> PRT

<213> Acinetobacter baumannii

<400> 76

Met Ala Thr Ala Val Arg Val Lys Asp Glu Val Asp Ala Ile Val Leu

1 5 10 15

Gly Asn Thr Leu Ala Met Glu Gly Gly Leu Asn Val Leu Lys Asp Ser

20 25 30

Ile Tyr Tyr Glu Pro Val Asp Gln Ser Thr Lys Leu Asn Val Asn Ile

35 40 45

Leu Ala Thr Ala Glu Ser Arg Lys Asp Asp Pro Val Leu Gln Lys Val

50 55 60

Gly Gln Leu Tyr His Thr Glu Ala Val Lys Lys Tyr Val Glu Gln His

65 70 75 80

Phe Gly Gly Thr Lys Val Asp Val Asn Gln Pro Ile Ser Tyr Leu Thr

85 90 95

Gln Ala Lys

<210> 77

<211> 169

<212> PRT

<213> Acinetobacter baumannii

<400> 77

Met Val Asn Glu Thr Met Lys His Gln Phe Lys Phe Asn Pro Leu Ala

1 5 10 15

Thr Ala Ile Phe Thr Leu Leu Cys Ser Gly Ser Ile Gln Ser Ser Tyr

20 25 30

Ala Glu Ser Ala Gly Val Val Ser Asn Ile Asp Asn Asn Gln Leu Lys

35 40 45

Ala Ser Ile Lys Glu Ala Tyr Pro Gly Gln Glu Phe Phe Gln Gln Tyr

50 55 60

Tyr Val Asp Lys Ser Ala Pro Glu Ala Gln Leu Arg Asn Asn Lys Tyr

65 70 75 80

Leu Ser Ser Ala Phe Cys Gln Gly Thr Trp Ile Thr Pro Ile Asn Pro

85 90 95

Glu Thr Lys Ala Leu Asp Ala Asp Lys Ala Thr Ser Val Val Thr Ala

100 105 110

Asp Tyr Gly His Tyr Asn Pro Ala Gly Asp Ser Val Leu Glu Gly Asn

115 120 125

Val Val Ile Asp Gln Glu Gly Arg Thr Val Arg Ala Asp Lys Val Thr

130 135 140

Ile Asp Lys Thr Gln Thr Phe Ala His Ala Gln Gly Arg Val Gln Leu

145 150 155 160

Ala Gln Gly Gly Leu Leu Ser Thr Lys

165

<210> 78

<211> 236

<212> PRT

<213> Acinetobacter baumannii

<400> 78

Met Ser Arg Gly Thr Arg Thr Phe Glu Ile Tyr Arg Tyr Asp Pro Asp

1 5 10 15

Lys Asp Lys Ala Pro Tyr Met Gln Thr Phe Lys Leu Glu Leu Thr Asp

20 25 30

Lys His Arg Met Leu Leu Asp Ala Leu Leu Ala Leu Lys Val Gln Asp

35 40 45

Glu Thr Leu Thr Phe Arg Arg Ser Cys Arg Glu Gly Ile Cys Gly Ser

50 55 60

Asp Gly Val Asn Ile Asn Gly Lys Asn Gly Leu Ala Cys Leu Trp Asn

65 70 75 80

Leu Asn Asp Leu Pro Glu Lys Ile Val Ile Arg Pro Leu Pro Gly Leu

85 90 95

Pro Val Ile Lys Asp Leu Val Val Asp Met Asn Gln Phe Tyr Asp Gln

100 105 110

Tyr Asp Lys Ile Gln Pro Phe Leu Ile Asn Asn Gln Pro Ala Pro Pro

115 120 125

Lys Glu Arg Leu Gln Ser Pro Glu Glu Arg Glu His Leu Asn Gly Leu

130 135 140

Tyr Glu Cys Ile Leu Cys Ala Cys Cys Ser Thr Ser Cys Pro Ser Phe

145 150 155 160

Trp Trp Asn Pro Asp Lys Phe Leu Gly Pro Ser Ala Leu Leu Asn Ala

165 170 175

Tyr Arg Phe Ile Ile Asp Ser Arg Asp Thr Ala Thr Gln Asp Arg Leu

180 185 190

Ser Arg Leu Asp Asp Pro Phe Ser Leu Phe Arg Cys Lys Gly Ile Met

195 200 205

Asn Cys Val Ser Val Cys Pro Lys Gly Leu Asn Pro Thr Lys Ala Ile

210 215 220

Gly His Ile Arg Asn Met Leu Phe Asp Gln Ala Gly

225 230 235

<210> 79

<211> 300

<212> PRT

<213> Acinetobacter baumannii

<400> 79

Met Leu Gly Lys Asp Gln Pro Val Ile Leu Gln Leu Leu Glu Val Pro

1 5 10 15

Val Glu Lys Ala Gln Gln Ala Leu Lys Gly Val Met Met Glu Leu Asp

20 25 30

Asp Cys Ala Phe Pro Leu Leu Ala Gly Met Ile Gly Thr Asp Asp Pro

35 40 45

Lys Val Ala Phe Lys Asp Ala Asp Tyr Ala Leu Leu Val Gly Ser Arg

50 55 60

Pro Arg Gly Pro Gly Met Glu Arg Ala Asp Leu Leu Lys Val Asn Gly

65 70 75 80

Glu Ile Phe Ile Gly Gln Gly Gln Ala Leu Asn Glu Val Ala Ser Arg

85 90 95

Asp Val Lys Val Leu Val Val Gly Asn Pro Ala Asn Thr Asn Ala Tyr

100 105 110

Ile Ala Met Lys Ser Ala Pro Asp Leu Pro Ala Lys Asn Phe Thr Ala

115 120 125

Met Leu Arg Leu Asp His Asn Arg Ala Leu Thr Gln Val Ala Gln Lys

130 135 140

Ala Gly Val Ala Val Ala Asp Ile Glu Lys Leu Thr Val Trp Gly Asn

145 150 155 160

His Ser Pro Thr Met Tyr Ala Asp Tyr Arg Phe Ala Thr Ala Asn Gly

165 170 175

Glu Ser Leu Lys Asp Lys Ile Asn Asp Pro Ala Trp Asn Lys Asp Val

180 185 190

Phe Leu Pro Thr Val Gly Lys Arg Gly Ala Ala Ile Ile Glu Ala Arg

195 200 205

Gly Leu Ser Ser Ala Ala Ser Ala Ala Asn Ala Ala Ile Asp His Met

210 215 220

Arg Asp Trp Ala Leu Gly Thr Asn Gly Lys Trp Val Thr Met Gly Val

225 230 235 240

Pro Ser Asp Gly Ser Tyr Gly Ile Pro Glu Gly Val Met Phe Gly Phe

245 250 255

Pro Val Thr Thr Glu Asn Gly Glu Tyr Lys Ile Val Gln Gly Leu Glu

260 265 270

Ile Asp Glu Phe Ser Arg Glu Arg Ile Asn Phe Thr Leu Asn Glu Leu

275 280 285

Glu Glu Glu Arg Ala Ala Ile Ala Asp Met Val Lys

290 295 300

<210> 80

<211> 373

<212> PRT

<213> Acinetobacter baumannii

<400> 80

Met Gly Glu Pro Val Ser Arg Asp Glu Val Ile Cys Asp Ile Glu Thr

1 5 10 15

Asp Lys Val Val Leu Glu Val Val Ala Pro Ala Asp Gly Ser Leu Val

20 25 30

Ala Ile Ile Lys Gly Glu Gly Asp Thr Val Leu Ser Asp Glu Val Ile

35 40 45

Ala Gln Phe Glu Ala Gly Ala Gly Ala Ala Ala Ala Ala Pro Ala Ala

50 55 60

Val Glu Gln Ala Val Ala Gln Thr Gln Ala Gly Ala Ala Pro Val Val

65 70 75 80

Glu Arg Asn Glu Thr Val Ser Asp Gln Ala Pro Ala Val Arg Lys Ala

85 90 95

Leu Thr Glu Ser Gly Ile Ala Ala Ser Asp Val Gln Gly Thr Gly Arg

100 105 110

Gly Gly Arg Ile Thr Lys Glu Asp Val Ala Asn His Gln Ala Lys Pro

115 120 125

Ala Ala Asn Val Thr Pro Leu Ser Val Ala Val Gly Glu Arg Ile Glu

130 135 140

Lys Arg Val Pro Met Thr Arg Leu Arg Lys Arg Val Ala Glu Arg Leu

145 150 155 160

Leu Ala Ala Thr Gln Glu Thr Ala Met Leu Thr Thr Phe Asn Glu Val

165 170 175

Asn Met Lys Pro Ile Met Glu Leu Arg Lys Gln Tyr Lys Asp Ala Phe

180 185 190

Glu Lys Arg His Gly Ala Arg Leu Gly Phe Met Ser Phe Phe Val Lys

195 200 205

Ala Ala Thr Glu Ala Leu Lys Arg Tyr Pro Ala Val Asn Ala Ser Ile

210 215 220

Asp Gly Asp Asp Ile Val Tyr His Gly Tyr Tyr Asp Ile Gly Val Ala

225 230 235 240

Val Ser Ser Asp Arg Gly Leu Val Val Pro Val Leu Arg Asp Thr Asp

245 250 255

Arg Met Ser Tyr Ala Glu Val Glu Ala Gly Ile Ala Ala Tyr Ala Ala

260 265 270

Lys Ala Arg Asp Gly Lys Leu Ser Ile Glu Glu Met Thr Gly Gly Thr

275 280 285

Phe Thr Ile Thr Asn Gly Gly Thr Phe Gly Ser Leu Leu Ser Thr Pro

290 295 300

Ile Leu Asn Gln Pro Gln Thr Gly Ile Leu Gly Met His Lys Ile Gln

305 310 315 320

Glu Arg Pro Met Ala Val Asn Gly Gln Val Glu Ile Leu Pro Met Met

325 330 335

Tyr Leu Ala Leu Ser Tyr Asp His Arg Met Ile Asp Gly Lys Glu Ala

340 345 350

Val Gly Phe Leu Val Ala Ile Lys Glu Leu Leu Glu Glu Pro Ala Lys

355 360 365

Leu Ile Leu Asp Leu

370

<210> 81

<211> 468

<212> PRT

<213> Acinetobacter baumannii

<400> 81

Met Ser Phe Ser Met Thr Lys Leu Ser Ala Ala Leu Leu Leu Thr Ser

1 5 10 15

Ser Leu Val Gly Cys Ala Ala Val Val Lys Thr Pro Tyr Gln Ala Pro

20 25 30

Ala Val Gln Val Pro Gly Ser Phe Gln Tyr Asp Lys Ala Lys Ala Lys

35 40 45

Thr Ala Ser Val Glu Gln Tyr Ser Asp Arg Trp Trp Thr Leu Phe Gly

50 55 60

Asp Thr Gln Leu Asn Gln Leu Val Thr Asn Val Leu Glu Arg Asn Ser

65 70 75 80

Asp Leu Ala Val Ala Gly Ile Thr Leu Gln Gln Ala Arg Leu Gln Ala

85 90 95

Asp Leu Thr Ala Asn Lys Gln Gly Leu Arg Thr Ser Ser Ser Val Ser

100 105 110

Thr Gly His Ser Phe Asp Leu Asn Ser Gly Asp Asp Ser Ala Lys Gly

115 120 125

Leu Ser Met Ser Ala Gly Val Ser Tyr Glu Leu Asp Leu Phe Gly Lys

130 135 140

Leu Ala Arg Gln Thr Glu Ala Ser Lys Trp Glu Ala Leu Ala Thr Glu

145 150 155 160

Gln Asp Leu Gln Ala Thr Gly Gln Ser Leu Ile Ala Thr Thr Ala Lys

165 170 175

Leu Tyr Trp Gln Leu Gly Tyr Leu Asn Glu Arg Tyr Ala Thr Ala Gln

180 185 190

Gln Ser Leu Ala Thr Ser Gln Lys Leu Tyr Gln Leu Val Gln Thr Gln

195 200 205

Tyr Lys Ala Gly Ala Val Ser Gly Leu Asp Leu Thr Gln Ala Glu Gln

210 215 220

Ser Val Gln Ser Gln Lys Ala Ser Leu Ser Gln Ile Glu Gln Gln Leu

225 230 235 240

Val Glu Thr Arg Thr Ala Ile Ala Val Leu Leu His Glu Pro Leu Gln

245 250 255

Gln Leu Asn Ile Gln Glu Pro Gln Arg Leu Pro Arg Thr Ala Leu Pro

260 265 270

Ala Ile Gly Ala Gly Leu Pro Ala Asp Ile Leu Ser Arg Arg Pro Asp

275 280 285

Leu Gln Ala Ala Glu Leu Arg Leu Arg Lys Ala Leu Ala Thr Lys Asp

290 295 300

Ala Thr Lys Ala Ser Tyr Tyr Pro Ser Ile Ser Leu Thr Ser Ser Leu

305 310 315 320

Gly Ser Ser Ser Thr Ser Leu Thr Glu Leu Leu Arg Asn Pro Ala Leu

325 330 335

Thr Leu Gly Ala Ser Leu Ser Leu Pro Phe Leu Gln Tyr Asn Asp Met

340 345 350

Lys Lys Asp Ile Ala Ile Ser Asn Leu Asp Tyr Glu Lys Ala Ile Ile

355 360 365

Gln Tyr Arg Gln Thr Leu Tyr Gln Ala Phe Ala Asp Val Glu Asn Ala

370 375 380

Leu Ser Ser Arg Thr Glu Leu Asp Lys Gln Val Ala Leu Gln Glu Arg

385 390 395 400

Asn Val Glu Leu Ala Glu Lys Thr Glu Arg Leu Thr Glu Val Arg Tyr

405 410 415

Arg Tyr Gly Ala Val Ala Leu Lys Thr Leu Leu Asp Ala Gln Gln Thr

420 425 430

Thr Arg Thr Ala Arg Leu Ser Leu Val Glu Thr Lys Gln Ser Gln Tyr

435 440 445

Asn Ala Tyr Val Thr Leu Met Gln Ala Leu Gly Gly Ser Pro Val Lys

450 455 460

Glu Leu Pro Gln

465

<210> 82

<211> 358

<212> PRT

<213> Acinetobacter baumannii

<400> 82

Met Ala Tyr Ala Asp Thr Leu Ala Pro Val Lys Pro Ala Ser Val Asp

1 5 10 15

Ala Cys Val Ala Leu Ala Ser Asn Ala Asp Arg Leu Ala Cys Tyr Asp

20 25 30

Ala Val Phe Lys Pro Ser Ala Leu Pro Val Val Gln Ala Ala Val Val

35 40 45

Pro Glu Pro Val Lys Lys Ile Asp Lys Pro Val Val Gln Pro Glu Thr

50 55 60

Phe Lys Glu Lys Val Val Asp Lys Val Ser Asn Ile Lys Val Ile Gly

65 70 75 80

Lys Ala Pro Thr Leu Glu Pro Thr Thr Ser Leu Leu Asp Gln Arg Trp

85 90 95

Glu Leu Ser Glu Lys Ser Lys Leu Gly Val Trp Asn Ile Arg Ala Tyr

100 105 110

Gln Pro Val Tyr Leu Leu Pro Val Phe Trp Thr Ser Asp Lys Asn Glu

115 120 125

Phe Pro Ser Ser Pro Asn Pro Asn Asn Thr Val Thr Glu Ala Gln Asn

130 135 140

Leu Lys Ser Thr Glu Ser Lys Phe Gln Ile Ser Leu Lys Thr Lys Ala

145 150 155 160

Trp Glu Asn Ile Phe Gly Asn Asn Gly Asp Leu Trp Val Gly Tyr Thr

165 170 175

Gln Ser Ser Arg Trp Gln Thr Phe Asn Ala Glu Glu Ser Arg Pro Phe

180 185 190

Arg Glu Thr Asn Tyr Glu Pro Glu Ala Ser Leu Met Phe Arg Thr Asn

195 200 205

Tyr Glu Leu Leu Gly Leu Asp Gly Arg Leu Leu Gly Val Thr Leu Asn

210 215 220

His Gln Ser Asn Gly Arg Ser Asp Pro Leu Ser Arg Ser Trp Asn Arg

225 230 235 240

Val Ile Phe Asn Val Gly Leu Glu Arg Gly Asn Phe Ala Leu Met Leu

245 250 255

Arg Pro Trp Ile Arg Leu Glu Glu Asp Ser Lys Asp Asp Asn Asn Pro

260 265 270

Asp Met Glu Asp Tyr Ile Gly Arg Gly Asp Leu Thr Ala Phe Tyr Lys

275 280 285

Trp Lys Gln Asn Asp Phe Ser Leu Met Leu Arg His Ser Leu Lys Gly

290 295 300

Gly Asp Asp Ser His Gly Ala Val Gln Phe Asp Trp Ala Phe Pro Ile

305 310 315 320

Ser Gly Lys Leu Arg Gly His Phe Gln Leu Phe Asn Gly Tyr Gly Glu

325 330 335

Ser Leu Ile Asp Tyr Asn His Arg Ala Thr Tyr Ala Gly Leu Gly Val

340 345 350

Ser Leu Met Asn Trp Tyr

355

<210> 83

<211> 108

<212> PRT

<213> Acinetobacter baumannii

<400> 83

Met Glu Gly Leu Ser Val Thr Tyr Asn Gly Ser Thr Asp Thr Ala Ile

1 5 10 15

Ile Lys Gly Gln Val Gln Ser Gln Ala Asp Lys Glu Lys Ile Ile Leu

20 25 30

Ile Val Gly Asn Val Asp His Val Ala Gln Val Asp Asp Gln Met Thr

35 40 45

Val Ala Thr Pro Glu Pro Glu Ser Lys Phe Tyr Thr Val Lys Ser Gly

50 55 60

Asp Asn Leu Ser Lys Ile Ala Lys Glu Phe Tyr Gly Asp Ala Asn Gln

65 70 75 80

Tyr Gln Lys Ile Phe Glu Ala Asn Lys Pro Met Leu Lys Asp Pro Asp

85 90 95

Glu Ile Phe Pro Gly Gln Val Leu Arg Ile Pro Gln

100 105

<210> 84

<211> 291

<212> PRT

<213> Acinetobacter baumannii

<400> 84

Met Thr Ala Ile Thr Ala Ser Met Val Lys Glu Leu Arg Asp Arg Thr

1 5 10 15

Gly Leu Ala Met Met Glu Cys Lys Lys Ala Leu Thr Glu Ala Asn Gly

20 25 30

Asp Ile Glu Leu Ala Ile Asp Asn Leu Arg Lys Ser Gly Gln Ala Lys

35 40 45

Ala Ala Lys Lys Ala Gly Asn Ile Ala Ala Asp Gly Ala Ile Thr Ile

50 55 60

Val Gln Asp Gly Asn Lys Ala Ile Leu Val Glu Val Asn Cys Gln Thr

65 70 75 80

Asp Phe Val Ala Lys Asp Glu Asn Phe Ser Asn Phe Ala His Thr Val

85 90 95

Ala Ala Ala Ala Leu Ala Ala Gly Glu Thr Asp Ala Ala Lys Ile Ala

100 105 110

Glu Leu Lys Leu Ala Asp Gly Gln Ser Val Glu Glu Ala Arg Ile Ala

115 120 125

Leu Val Gln Lys Ile Gly Glu Asn Ile Gln Val Arg Arg Ala Lys Ile

130 135 140

Val Glu Gly Glu Gln Leu Ala Ile Tyr Lys His Gly Leu Lys Ile Gly

145 150 155 160

Val Val Val Ser Tyr Thr Gly Asp Ala Asp Thr Gly Lys Gly Ile Ala

165 170 175

Met His Val Ala Ala Phe Asn Pro Val Ala Val Asn Ala Glu Ala Val

180 185 190

Pro Ala Asp Leu Ile Ala Lys Glu Lys Glu Ile Ala Glu Ala Lys Ala

195 200 205

Leu Glu Ser Gly Lys Pro Ala Asn Ile Val Glu Lys Met Val Thr Gly

210 215 220

Ser Val Glu Lys Tyr Leu Asn Glu Val Ala Leu Asp Arg Gln Met Tyr

225 230 235 240

Val Ile Asp Asn Glu Lys Lys Val Ala Asp Val Leu Lys Ala Thr Gly

245 250 255

Thr Asn Val Ala Asn Phe Val Arg Phe Glu Val Gly Glu Gly Ile Glu

260 265 270

Lys Lys Ala Glu Leu Ser Phe Ala Glu Glu Val Ala Ala Ala Gln Ala

275 280 285

Ala Ala Lys

290

<210> 85

<211> 170

<212> PRT

<213> Acinetobacter baumannii

<400> 85

Met Thr Gly Cys Ala Ser Arg Lys Pro Ala Thr Thr Ala Thr Thr Gly

1 5 10 15

Thr Thr Asn Pro Ser Thr Val Asn Thr Thr Gly Leu Ser Glu Asp Ala

20 25 30

Ala Leu Asn Ala Gln Asn Leu Ala Gly Ala Ser Ser Lys Gly Val Thr

35 40 45

Glu Ala Asn Lys Ala Ala Leu Ala Lys Arg Val Val His Phe Asp Tyr

50 55 60

Asp Ser Ser Asp Leu Ser Thr Glu Asp Tyr Gln Thr Leu Gln Ala His

65 70 75 80

Ala Gln Phe Leu Met Ala Asn Ala Asn Ser Lys Val Ala Leu Thr Gly

85 90 95

His Thr Asp Glu Arg Gly Thr Arg Glu Tyr Asn Met Ala Leu Gly Glu

100 105 110

Arg Arg Ala Lys Ala Val Gln Asn Tyr Leu Ile Thr Ser Gly Val Asn

115 120 125

Pro Gln Gln Leu Glu Ala Val Ser Tyr Gly Lys Glu Ala Pro Val Asn

130 135 140

Pro Gly His Asp Glu Ser Ala Trp Lys Glu Asn Arg Arg Val Glu Ile

145 150 155 160

Asn Tyr Glu Ala Val Pro Pro Leu Leu Lys

165 170

<210> 86

<211> 144

<212> PRT

<213> Acinetobacter baumannii

<400> 86

Met Lys Lys Ile Ile Phe Leu Gly Leu Ala Leu Val Ser Leu Thr Ala

1 5 10 15

Cys Ser Ser Val Gln His Lys Asp Ser Thr Pro Pro Lys Ile Gly Ser

20 25 30

Pro Asn Pro Ala Ser Gln Tyr Cys Val Glu Gln Gly Gly Lys Leu Glu

35 40 45

Ile Arg Asn Glu Ala Asn Gly Gln Val Gly Tyr Cys His Leu Pro Asn

50 55 60

Gly Gln Val Val Glu Glu Trp Lys Leu Phe Arg Asp Asn Gln Ala Asn

65 70 75 80

Cys Val Ser Glu Glu Ala Gln Lys Leu Val Gly Leu Ser Gly Leu Thr

85 90 95

Asp Asp Gln Ile Lys Gln Lys Thr Lys Ser Glu Ile Val Arg Lys Val

100 105 110

Ala Pro Gly Gln Pro Met Thr Met Asp Tyr Arg Ser Asn Arg Val Thr

115 120 125

Val Thr Ile Asp Pro Thr Ser Lys Lys Ile Thr Gln Ala Thr Cys Gly

130 135 140

<210> 87

<211> 249

<212> PRT

<213> Acinetobacter baumannii

<400> 87

Met Lys Val Leu Arg Val Leu Val Thr Thr Thr Ala Leu Leu Ala Ala

1 5 10 15

Gly Ala Ala Met Ala Asp Glu Ala Val Val His Asp Ser Tyr Ala Phe

20 25 30

Asp Lys Asn Gln Leu Ile Pro Val Gly Ala Arg Ala Glu Val Gly Thr

35 40 45

Thr Gly Tyr Gly Gly Ala Leu Leu Trp Gln Ala Asn Pro Tyr Val Gly

50 55 60

Leu Ala Leu Gly Tyr Asn Gly Gly Asp Ile Ser Trp Thr Asp Asp Val

65 70 75 80

Ser Val Asn Gly Thr Lys Tyr Asp Leu Asp Met Asp Asn Asn Asn Val

85 90 95

Tyr Leu Asn Ala Glu Ile Arg Pro Trp Gly Ala Ser Thr Asn Pro Trp

100 105 110

Ala Gln Gly Leu Tyr Ile Ala Ala Gly Ala Ala Tyr Leu Asp Asn Asp

115 120 125

Tyr Asp Leu Ala Lys Arg Ile Gly Asn Gly Asp Thr Leu Ser Ile Asp

130 135 140

Gly Lys Asn Tyr Gln Gln Ala Val Pro Gly Gln Glu Gly Gly Val Arg

145 150 155 160

Gly Lys Met Ser Tyr Lys Asn Asp Ile Ala Pro Tyr Leu Gly Phe Gly

165 170 175

Phe Ala Pro Lys Ile Ser Lys Asn Trp Gly Val Phe Gly Glu Val Gly

180 185 190

Ala Tyr Tyr Thr Gly Asn Pro Lys Val Glu Leu Thr Gln Tyr Asn Leu

195 200 205

Ala Pro Val Thr Gly Asn Pro Thr Ser Ala Gln Asp Ala Val Asp Lys

210 215 220

Glu Ala Asn Glu Ile Arg Asn Asp Asn Lys Tyr Glu Trp Met Pro Val

225 230 235 240

Gly Lys Val Gly Val Asn Phe Tyr Trp

245

<210> 88

<211> 999

<212> PRT

<213> Acinetobacter baumannii

<400> 88

Met Arg Ala Val Glu Gly Asp Arg Leu Leu Ile Leu Lys His Gly Asp

1 5 10 15

Val Trp Lys Arg Met Ala Val Gly Phe Lys Met Asp Leu Asn His Trp

20 25 30

Asp Pro Arg Ile Glu Ala Gln Arg Ser Trp Phe Ile Ser Arg Gln Pro

35 40 45

Tyr Leu Asp Arg Leu Ser Ala Arg Ala Ser Arg Tyr Leu Tyr His Thr

50 55 60

Val Lys Glu Ala Glu Arg Arg Gly Leu Pro Thr Glu Leu Ala Leu Leu

65 70 75 80

Pro Val Ile Glu Ser Ser Tyr Asp Pro Ala Ala Thr Ser Ser Ala Ala

85 90 95

Ala Ala Gly Leu Trp Gln Phe Ile Pro Ser Thr Gly Arg Ile Tyr Gly

100 105 110

Leu Gln Gln Thr Gly Met Tyr Asp Gly Arg Arg Asp Val Val Glu Ser

115 120 125

Thr Arg Ala Ala Tyr Glu Phe Leu Gly Ser Leu Tyr Asn Gln Phe Gly

130 135 140

Ser Trp Glu Leu Ala Leu Ala Ala Tyr Asn Ala Gly Pro Gly Arg Ile

145 150 155 160

Gln Gln Ala Ile Asn Arg Asn Gln Ala Ala Gly Leu Pro Thr Asp Tyr

165 170 175

Trp Ser Leu Lys Leu Pro Gln Glu Thr Met Asn Tyr Val Pro Arg Phe

180 185 190

Leu Ala Val Ala Gln Ile Ile Lys Asn Pro Arg Ala Tyr Gly Val Ser

195 200 205

Leu Pro Pro Ile Ala Asn Arg Pro His Phe Arg Glu Val Thr Leu Ser

210 215 220

Ala Pro Leu Ser Leu Asn Glu Ile Ala Ser Val Thr Gly Leu Ser Arg

225 230 235 240

Ala Glu Leu Tyr Ala Leu Asn Pro Gly Tyr Arg Gly Glu Thr Val Asp

245 250 255

Pro Ala Ser Pro Met Arg Ile Leu Ile Pro Ala Asp Ile Ser Pro Ser

260 265 270

Val Asp Asn Lys Leu Lys Gly Met Lys Ala Gly Gly Ser Ser Gly Trp

275 280 285

Trp Ala Ser Val Thr Ser Pro Ser Lys Pro Thr Thr Thr Thr Ser Thr

290 295 300

Ser Val Thr Val Arg Thr Thr Pro Ser Thr Pro Ala Gln Pro Val Arg

305 310 315 320

Pro Ser Thr Pro Ala Lys Thr Ser Ser Ser Ser Val Thr Val Lys Thr

325 330 335

Ala Thr Pro Arg Gly Ser Asp Ala Leu Ala Ala Phe Ala Ala Ser Ala

340 345 350

Asp Val Pro Ser Ala Pro Arg Ile Pro Val Ala Val Thr Pro Ala Ala

355 360 365

Asn Ile Lys Pro Val Arg Thr Glu Pro Pro Ile Ser Ala Thr Glu Arg

370 375 380

Glu Lys Ile Leu Ala Ala Val Arg Ala Glu Gly Glu Lys Glu Thr Val

385 390 395 400

Asp Gln Ala Leu Glu Pro Gln Ala Thr Gln Ala Glu Lys Asp Gln Val

405 410 415

Val Ala Glu Leu Lys Ala Leu Ala Pro Gln Gly Thr Glu Ile Val Asp

420 425 430

Pro Tyr Asp Gly Lys Ile Lys Leu Thr Ala Ile Gln Thr Ser Gln Ser

435 440 445

Val Ala Glu Gln Gln Gly Lys Glu Val Ser Lys Gly Phe Ala Tyr Pro

450 455 460

Lys Thr Leu Ala Glu Asp Ala Thr Leu Ala Asn Ser Glu Asp Ala Gln

465 470 475 480

Arg Asn Lys Asp Lys Pro Tyr Ile Lys Thr Asp Thr Asp Val Val Val

485 490 495

Val Gln Pro Lys Gly Lys Arg Ser Thr Tyr Thr Val Gln Pro Gly Asp

500 505 510

Thr Leu Ala Val Ile Ala Met Lys Asn Gly Val Asn Trp Arg Asp Val

515 520 525

Ala Lys Trp Asn Gln Ile Asp Pro Glu Lys Thr Leu Phe Val Gly Thr

530 535 540

Ser Leu Tyr Leu Tyr Asp Ala Lys Pro Gln Glu Ala Glu Thr Thr Ala

545 550 555 560

Lys Ser Ala Ala Lys Pro Asp Val Tyr Val Val Gln Ala Asn Asp Ser

565 570 575

Leu Thr Gly Val Ala Asn Gln Phe Asn Leu Ser Val Lys Gln Leu Ala

580 585 590

Glu Tyr Asn Asp Leu Ser Val Thr Asp Gly Leu Phe Val Gly Gln Lys

595 600 605

Leu Gln Leu Lys Glu Pro Lys Gly Asn Arg Ala Ala Lys Val Glu Pro

610 615 620

Lys Ala Ile Gln Ala Ser Thr Arg Arg Ile Ala Thr Lys Ser Tyr Thr

625 630 635 640

Val Lys Arg Gly Glu Tyr Leu Lys Leu Ile Ala Asp Arg Tyr Ala Leu

645 650 655

Ser Asn Gln Glu Leu Ala Asp Leu Thr Pro Gly Leu Thr Ala Gly Ser

660 665 670

Asn Leu Ile Val Gly Gln Lys Ile Asn Val Pro Ala Lys Glu Ile Thr

675 680 685

Val Asp Glu Val Asp Asp Ser Lys Ala Ser Gly Lys Tyr Glu Lys Leu

690 695 700

Ala Ala Gly Pro Ser Tyr Lys Thr Glu Ser Tyr Lys Val Gln Arg Gly

705 710 715 720

Asp Thr Leu Ser Ser Ile Ala Thr Lys Ser Lys Ile Ser Leu Ala Glu

725 730 735

Leu Ala Glu Leu Asn Asn Leu Lys Ala Asn Ser His Val Gln Leu Gly

740 745 750

Gln Thr Leu Lys Val Pro Ala Gly Ala Ser Val Pro Asp Gln Tyr Val

755 760 765

Val Gln Ser Gly Asp Ser Leu Asn Ala Ile Ala Ala Lys Tyr Asn Leu

770 775 780

Gln Thr Ser Tyr Leu Ala Asp Leu Asn Gly Leu Ser Arg Thr Ala Gly

785 790 795 800

Leu Arg Ala Gly Gln Arg Leu Lys Leu Thr Gly Glu Val Glu Thr Thr

805 810 815

Ser Lys Val Ser Ala Lys Asn Thr Lys Glu Glu Thr Pro Glu Thr Tyr

820 825 830

Thr Val Lys Ser Gly Asp Ser Leu Gly Asn Ile Ala Asn Arg Tyr His

835 840 845

Leu Gln Leu Asp Tyr Leu Ala Ala Leu Asn Gly Leu Ser Arg Asn Ser

850 855 860

Asn Val Arg Val Gly Gln Arg Leu Lys Leu Thr Gly Asp Leu Pro Thr

865 870 875 880

Val Glu Thr Ala Lys Thr Asp Thr Ala Lys Ser Ser Pro Lys Ala Val

885 890 895

Val Ala Gly Lys Asn Thr Glu Lys Tyr Thr Val Lys Ala Gly Glu Ser

900 905 910

Leu Asn Ala Ile Ala Ser Arg Ala Gly Ile Ser Val Arg Glu Leu Ala

915 920 925

Glu Met Asn Ala Leu Lys Ala Asn Ala Asn Leu Gln Arg Gly Gln Asn

930 935 940

Ile Val Ile Pro Lys Thr Val Val Glu Tyr Lys Val Lys Arg Gly Asp

945 950 955 960

Thr Leu Ile Gly Leu Ala Ser Lys Tyr Gly Leu Glu Thr Thr Leu Leu

965 970 975

Ala Glu Leu Asn Asn Leu Thr Pro Ser Thr Gln Leu Arg Ile Gly Asp

980 985 990

Ile Ile Lys Val Pro Asn Leu

995

<210> 89

<211> 519

<212> PRT

<213> Acinetobacter baumannii

<400> 89

Met Arg Asn Ser Ala Met Gln Gln Leu Asn Pro Ser Glu Ile Ser Ala

1 5 10 15

Leu Ile Lys Gln Arg Ile Gly Asp Leu Asp Thr Ser Ala Thr Ala Lys

20 25 30

Asn Glu Gly Thr Ile Val Met Val Ser Asp Gly Ile Val Arg Ile His

35 40 45

Gly Leu Ala Asp Ala Met Tyr Gly Glu Met Ile Glu Phe Asp Gly Gly

50 55 60

Leu Phe Gly Met Ala Leu Asn Leu Glu Gln Asp Ser Val Gly Ala Val

65 70 75 80

Val Leu Gly Asn Tyr Leu Ser Leu Gln Glu Gly Gln Lys Ala Arg Cys

85 90 95

Thr Gly Arg Val Leu Glu Val Pro Val Gly Pro Glu Leu Leu Gly Arg

100 105 110

Val Val Asp Ala Leu Gly Asn Pro Ile Asp Gly Lys Gly Pro Ile Asp

115 120 125

Ala Lys Leu Thr Asp Ala Val Glu Lys Val Ala Pro Gly Val Ile Trp

130 135 140

Arg Gln Ser Val Asp Glu Pro Val Gln Thr Gly Tyr Lys Ser Val Asp

145 150 155 160

Thr Met Ile Pro Val Gly Arg Gly Gln Arg Glu Leu Ile Ile Gly Asp

165 170 175

Arg Gln Thr Gly Lys Thr Ala Met Ala Ile Asp Ala Ile Ile Ala Gln

180 185 190

Lys His Ser Gly Ile Lys Cys Val Tyr Val Ala Ile Gly Gln Lys Gln

195 200 205

Ser Thr Ile Ala Asn Val Val Arg Lys Leu Glu Glu Thr Gly Ala Met

210 215 220

Ala Tyr Thr Thr Val Val Ala Ala Ala Ala Ala Asp Pro Ala Ala Met

225 230 235 240

Gln Tyr Leu Ala Pro Tyr Ser Gly Cys Thr Met Gly Glu Tyr Phe Arg

245 250 255

Asp Arg Gly Glu Asp Ala Leu Ile Ile Tyr Asp Asp Leu Ser Lys Gln

260 265 270

Ala Val Ala Tyr Arg Gln Ile Ser Leu Leu Leu Arg Arg Pro Pro Gly

275 280 285

Arg Glu Ala Tyr Pro Gly Asp Val Phe Tyr Leu His Ser Arg Leu Leu

290 295 300

Glu Arg Ala Ser Arg Val Ser Ala Asp Tyr Val Glu Lys Phe Thr Asn

305 310 315 320

Gly Ala Val Thr Gly Gln Thr Gly Ser Leu Thr Ala Leu Pro Ile Ile

325 330 335

Glu Thr Gln Ala Gly Asp Val Ser Ala Phe Val Pro Thr Asn Val Ile

340 345 350

Ser Ile Thr Asp Gly Gln Ile Phe Leu Glu Thr Ser Leu Phe Asn Ala

355 360 365

Gly Ile Arg Pro Ala Val Asn Ala Gly Ile Ser Val Ser Arg Val Gly

370 375 380

Gly Ser Ala Gln Thr Lys Ile Ile Lys Lys Leu Ser Gly Gly Ile Arg

385 390 395 400

Thr Ala Leu Ala Gln Tyr Arg Glu Leu Ala Ala Phe Ala Gln Phe Ala

405 410 415

Ser Asp Leu Asp Glu Ala Thr Arg Lys Gln Leu Glu His Gly Gln Arg

420 425 430

Val Thr Glu Leu Met Lys Gln Lys Gln Tyr Ala Pro Tyr Ser Ile Ala

435 440 445

Asp Gln Ala Val Ser Val Tyr Ala Ser Asn Glu Gly Tyr Met Ala Asp

450 455 460

Val Glu Val Lys Lys Ile Val Asp Phe Asp Ala Ala Leu Ile Ser Tyr

465 470 475 480

Phe Arg Ser Glu Tyr Ala Pro Leu Met Lys Gln Ile Asp Glu Thr Gly

485 490 495

Asp Tyr Asn Lys Asp Ile Glu Ala Ala Ile Lys Ser Gly Ile Glu Ser

500 505 510

Phe Lys Ala Thr Gln Thr Tyr

515

<210> 90

<400> 90

000

<210> 91

<400> 91

000

<210> 92

<400> 92

000

<210> 93

<400> 93

000

<210> 94

<400> 94

000

<210> 95

<400> 95

000

<210> 96

<400> 96

000

<210> 97

<400> 97

000

<210> 98

<400> 98

000

<210> 99

<400> 99

000

<210> 100

<211> 20

<212> DNA

<213> Artificial Sequence

<220>

<223> primer forward

<400> 100

gctaattggt gaaggtagtc 20

<210> 101

<211> 20

<212> DNA

<213> Artificial Sequence

<220>

<223> primer reverse

<400> 101

gacgaatcgt ttgaatctgc 20

Claims (15)

1.一种疫苗组合物，其适用于预防性治疗由鲍氏不动杆菌菌株在哺乳动物体内引起的感染，所述疫苗组合物包含：

a.脂多糖缺失的鲍氏不动杆菌菌株的耐粘菌素突变体，其特征在于选自由lpxA、lpxC、lpxD组成的组的基因部分或完全失活；和/或

b.衍生自如上段a)中定义的脂多糖缺失的鲍氏不动杆菌菌株的耐粘菌素突变体的外膜囊泡。

2.根据权利要求1所述的疫苗组合物，其特征在于，其中所述脂多糖缺失的鲍氏不动杆菌菌株的lpxD基因的核苷酸序列部分缺失。

3.根据权利要求1或2所述的疫苗组合物，其特征在于，所述疫苗还包含选自以下序列的重组多肽：

a)氨基酸序列SEQ ID NO.27或其片段，其中术语片段在此理解为生物活性片段，其选自SEQ ID NO.1至SEQ ID NO.11或其任何组合，或与序列SEQ ID NO.1至SEQ ID NO.11具有至少85％一致性的氨基酸序列；和/或

b)氨基酸序列SEQ ID No.28或其片段，其中术语片段在此理解为生物活性片段，其选自SEQ ID NO.12至SEQ ID NO.23或其任何组合，或与氨基酸序列SEQ ID NO.12至SEQ IDNO.23具有至少85％一致性的序列。

4.根据权利要求1或2所述的疫苗组合物，其特征在于，所述疫苗组合物还包含选自SEQID NO:29、SEQ ID NO:30、SEQ ID NO:31、SEQ ID NO:32、SEQ ID NO:33、SEQ ID NO:34、SEQID NO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ ID NO:38、SEQ ID NO:39、SEQ ID NO:40、SEQID NO:41、SEQ ID NO:42、SEQ ID NO:43、SEQ ID NO:44、SEQ ID NO:45、SEQ ID NO:46、SEQID NO:47、SEQ ID NO:48、SEQ ID NO:49、SEQ ID NO:50、SEQ ID NO:51、SEQ ID NO:52、SEQID NO:53、SEQ ID NO:54、SEQ ID NO:55、SEQ ID NO:56、SEQ ID NO:57、SEQ ID NO:58、SEQID NO:59、SEQ ID NO:60、SEQ ID NO:61、SEQ ID NO:62、SEQ ID NO:63、SEQ ID NO:64、SEQID NO:65、SEQ ID NO:66、SEQ ID NO:67、SEQ ID NO:68、SEQ ID NO:69、SEQ ID NO:70、SEQID NO:71、SEQ ID NO:72、SEQ ID NO:73、SEQ ID NO:74、SEQ ID NO:75、SEQ ID NO:76、SEQID NO:77、SEQ ID NO:78、SEQ ID NO:79、SEQ ID No:80、SEQ ID NO:81、SEQ ID NO:82、SEQID NO:83、SEQ ID NO:84、SEQ ID NO:85、SEQ ID NO:86、SEQ ID NO:87、SEQ ID NO:88、SEQID NO:89或其组合的鲍氏不动杆菌的纯化的外膜蛋白序列。

5.根据权利要求1或2所述的疫苗组合物，其特征在于，所述疫苗还包含SEQ ID NO.24或25的融合重组多肽或由SEQ ID NO.26的核酸分子编码的融合重组多肽。

6.根据权利要求1或2所述的疫苗组合物，其特征在于，所述脂多糖缺失的鲍氏不动杆菌菌株包含以下核苷酸序列：该核苷酸序列能够编码氨基酸序列SEQ ID Nos.1-89中的任何一个，以使所述菌株能够产生氨基酸序列SEQ ID Nos.1-89中的任何一个的外源表达。

7.根据权利要求1或2所述的疫苗组合物，其特征在于，所述疫苗组合物还包含载体，所述载体包含核苷酸序列，该核苷酸序列能够编码并且表达氨基酸序列SEQ ID Nos.1-89中的任何一个。

8.根据权利要求1或2所述的疫苗组合物，其特征在于，所述脂多糖缺失的鲍氏不动杆菌菌株被灭活。

9.根据权利要求1或2所述的疫苗组合物，其特征在于，所述脂多糖缺失的鲍氏不动杆菌菌株来自ATCC菌株19606。

10.根据权利要求1或2所述的疫苗组合物，其中，该疫苗组合物用于预防性治疗由鲍氏不动杆菌在哺乳动物体内引起的感染。

11.根据权利要求10所述的疫苗组合物，其中，所述哺乳动物为人类。

12.一种疫苗组合物，其包含分离的抗体或其片段，所述抗体或其片段是在用权利要求1或2所述的疫苗组合物对哺乳动物进行免疫处理后获得的，或者能够在进行该免疫处理后获得。

13.根据权利要求12所述的疫苗组合物在制备治疗由鲍氏不动杆菌在哺乳动物中引起的感染的药物上的用途。

14.根据权利要求13所述的用途，其中所述哺乳动物为人类。

15.脂多糖缺失的鲍氏不动杆菌菌株和/或由其衍生的外膜囊泡在生产能够结合所述菌株和/或外膜囊泡的抗体或其片段上的用途，所述脂多糖缺失的鲍氏不动杆菌菌株的特征在于选自由lpxA、lpxC、lpxD组成的组的基因的部分或完全失活。

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