CN107064076A - A kind of detecting system for susceptibility - Google Patents
A kind of detecting system for susceptibility Download PDFInfo
- Publication number
- CN107064076A CN107064076A CN201710037785.3A CN201710037785A CN107064076A CN 107064076 A CN107064076 A CN 107064076A CN 201710037785 A CN201710037785 A CN 201710037785A CN 107064076 A CN107064076 A CN 107064076A
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- Prior art keywords
- kit
- array
- aperture
- susceptibility
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/59—Transmissivity
Abstract
The present invention relates to a kind of detecting system for susceptibility, including white light LED array, optical collimating component, photosensitive array, signal transacting control centre, software analysis system.During detection, the LED in white light LED array is lighted and kept for several seconds one by one, and LED light is by optical collimating component, by multiple reflections, forms the stronger light beam of intensity of illumination, is irradiated on the aperture of kit and is penetrated aperture arrival photosensitive array.Photosensitive array changes into the power of the transmitted light detected the pulse of different frequency, is transferred to signal transacting control centre, pulse signal is changed into decimal data by signal transacting control centre, and sends software analysis system to.Software analysis system stores the data received sequentially in time, and timing sends detection signal, detects the turbidity of liquid in aperture during whole susceptibility.By the data record detected every time and it is depicted as the growth curve of bacterium.The drug resistance of bacterium is judged according to growth curve.With very strong creativeness.
Description
Technical field
The present invention relates to technical field of medical equipment, and in particular to a kind of detecting system for susceptibility.
Background technology
Because the drug resistance of microorganism is on the rise, infectious diseases needs precise Identification Species of Pathogens and tests its body
Outer Susceptibility to antibiotics (AST), medication reference is provided for clinic.Current clinic depends on manual method or commercialization
Analysis system is detected.Traditional manual method and commercialization analysis system, is, by diluting colonies certain multiple, to be added to
It has been coated with the antibiotic agent box of variety classes various concentrations, has been put into incubator and cultivates 8-12 hours, visually observe reagent
The turbidity of liquid in box aperture, or judge by way of instrument interpretation the drug resistance of bacterium.Culture and detection are separated, by
To the limitation and the limitation of traditional interpretation mode of naked eyes identification.Whole process is needed to consume a longer time, it is impossible to which satisfaction is faced
Requirement of the bed for this project quick detection.
The content of the invention
(1) technical problem solved
In view of the shortcomings of the prior art, the invention provides a kind of detecting system for susceptibility, set according to system,
During culture, the turbidity of liquid in timing detection kit aperture.By the data record detected every time and it is depicted as thin
The growth curve of bacterium.The drug resistance of bacterium is judged according to growth curve.
(2) technical scheme
To realize object above, the present invention is achieved by the following technical programs：
A kind of detecting system for susceptibility, it is characterised in that：Comprise the following steps：
Step 1: during detection, the LED in white light LED array is lighted and kept for several seconds one by one, and LED light passes through optical alignment
Part, by multiple reflections, forms the stronger light beam of intensity of illumination, is irradiated on the aperture of kit and penetrates aperture and reach and feels
Optical arrays.
Step 2: because antibiotic does not work for the bacterium to the antibiotics resistance, to the thin of the antibiotics resistance
Bacterium is bred rapidly during susceptibility, and kit aperture, which is mixed with antibiotic and the aperture to the bacterium of the antibiotics resistance, to be become
Muddiness, the transmitted light that photosensitive array is detected is weak, then breeds relatively slow to the bacterium of the antibiotic sensitive or even no longer breeds, photosensitive
The transmitted light that array detection is arrived is stronger, and photosensitive array changes into the power of the transmitted light detected the pulse of different frequency, passes
It is defeated by signal transacting control centre.
Step 3: pulse signal is changed into decimal data by signal transacting control centre, and send software analysis system to
System.
Step 4: software analysis system stores the data received sequentially in time, and timing sends detection signal,
The turbidity of liquid in aperture during whole susceptibility is detected, by the data record detected every time and the growth that is depicted as bacterium is bent
Line, the drug resistance of bacterium is judged according to growth curve.
It is preferred that, including white light LED array, optical collimating component, photosensitive array, signal management control centre and software point
Analysis system, in addition to telecontrol equipment, photoelectric sensor, LED drive circuit and multiplex electronics；The telecontrol equipment includes bottom
Plate, motor and displacement platform, motor, displacement platform are arranged on bottom plate center, are located on the same line, motor shaft passes through screw
Shaft coupling is fixedly connected with, the other end of shaft coupling is screwed in the rotating shaft for being connected to displacement platform, displacement platform turns
Close to motor one end by bridgeware on axle, screw is fixedly connected on kit pallet, kit pallet and sets multiple kits
Rest area, each kit rest area one group of white light LED array of correspondence, the size of kit rest area according to kit size come
It is determined that, multiple circular holes are left on the panel of kit rest area, are distributed in array-like, between quantity, size and the aperture of circular hole
Distance determine that there is a row dentation protrusion side of kit pallet, and the number of dentation protrusion is in kit by kit
The line number of aperture determines that the side of bottom plate is screwed a L-shaped metalwork, and position is protruded with the dentation of kit pallet
In the same side, some of L-shaped metalwork is highly screwed a U-shaped photoelectric sensor, is zero-bit photoelectric sensor, zero
The center of level sensor is centrally located at sustained height with dentation protrusion, and the both sides of bottom plate are screwed multiple doorframe shape gold
Belong to frame, the line number of number kit rest area on kit pallet of doorframe shape metal frame is determined, multiple doorframe shape metals
Frame is parallel to each other, and the doorframe shape metal frame side wall screw of neighbouring L-shaped metalwork fixes a U-shaped photoelectric sensor, is detecting position
Photoelectric sensor, detecting position photoelectric sensor is located at sustained height with zero-bit photoelectric sensor.
It is preferred that, the top of the doorframe shape metalwork is provided with white light LED array, optical collimating component, the optics
The underface of collimating components is photosensitive array.
It is preferred that, the white light LED array is positive white light cold light source, colour temperature 6000-7000K, every group of white light LED array
Number is determined that every group of white light LED array corresponds to one group of photosensitive array by the columns of kit.
It is preferred that, the optical collimating component is metal parts, and inside, which is scratched, multiple cylindrical holes, aperture inwall light
It is sliding, be conducive to the LED in the reflection of light, one white light LEDs of each aperture correspondence, optical driver circuit control white light LED array
Light and kept for several seconds one by one.
It is preferred that, a part of multiplex electronics by electric signal connect signal transacting control centre and LED drive circuit,
Another part multiplex electronics connect signal transacting control centre and photosensitive array by electric signal, with signal transacting control
The more white light LED array of the less output end control of the heart and photosensitive array.
It is preferred that, during detection, signal transacting control centre sends signal, and controlled motor is tried with being rotated forward compared with low velocity
The center of U-shaped detecting position photoelectric sensor is protruded through on agent box pallet away from motor side dentation, causes optoelectronic switch output electricity
Flat change, this level change is transferred to signal transacting control centre, and control centre sends instruction, LED drive circuit driving
The LED array on all metal frame tops is lighted one by one towards same direction, and the time lighted, drug sensitivity tests were optimal by many experiments
To determine, LED light passes through optical collimating component, by multiple reflections, forms the stronger light velocity of illumination and is irradiated to the small of kit
Hole simultaneously penetrates aperture arrival photosensitive array, and the transmitted light received is converted into pulse frequency by photosensitive array, and transmitted light is stronger, frequency
Rate is higher, and these frequencies are transferred to signal transacting control centre, and in signal transacting control centre, these frequencies are converted into ten
Binary data, is transferred to software analysis system, and software analysis system is stored and sorted to data sequentially in time.
It is preferred that, the signal transacting control centre has received kit the first row, and last detects data, the first row
Detection terminates, and signal processing control unit continues low speed and rotated forward, with protruding phase away from motor side dentation on kit pallet
The adjacent center for projecting through detecting position photoelectric sensor, starts to detect the second row.
(3) beneficial effect
The present invention is in detection, and the LED in white light LED array is lighted and kept for several seconds one by one, and LED light passes through optical alignment
Part, by multiple reflections, forms the stronger light beam of intensity of illumination, is irradiated on the aperture of kit and penetrates aperture and reach and feels
Optical arrays.Antibiotic is resistance to not to work for the bacterium to the antibiotics resistance, to the bacterium of the antibiotics resistance in susceptibility mistake
Bred rapidly in journey, kit aperture, which is mixed with antibiotic and the aperture to the bacterium of the antibiotics resistance, becomes muddy, photosensitive
The transmitted light that array detection is arrived is weak, then breeds relatively slow to the bacterium of the antibiotic sensitive or even no longer breeds, photosensitive array detection
The transmitted light arrived is stronger, and photosensitive array changes into the power of the transmitted light detected the pulse of different frequency, is transferred to signal
Control centre is handled, pulse signal is changed into decimal data by signal transacting control centre, and sends software analysis system to.
Software analysis system stores the data received sequentially in time, and timing sends detection signal, detects whole susceptibility mistake
In journey in aperture liquid turbidity.By the data record detected every time and it is depicted as the growth curve of bacterium.It is bent according to growth
Line judges the drug resistance of bacterium, and it is more accurate that it is detected, it is more convenient to operate, with very strong creativeness.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is the accompanying drawing used required in technology description to be briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is detection means profile of the invention；
Fig. 2 is the quick medicine-sensitive dimensional structure diagram of the susceptibility detection using the present invention；
Fig. 3 is control circuit structure block diagram of the invention；
Label in figure is represented respectively：
1. bottom plate, 2. motors, 3. displacement platforms, 4. bridgewares, 5. shaft couplings, 6.L shape metalworks, 7. zero-bit photoelectric sensings
Device, 8. gate-shaped metal frames, 9. detecting position photoelectric sensors, 10. white light LED arrays, 11. optical collimating components, 12. photosensitive battle arrays
Row, 13. kit pallets, 14. kits.
Embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with the embodiment of the present invention
In accompanying drawing, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is
A part of embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art
The every other embodiment obtained under the premise of creative work is not made, belongs to the scope of protection of the invention.
As Figure 1-3, the detection means for susceptibility includes bottom plate 1, motor 2, displacement platform 3, bridgeware 4, shaft coupling
Section 5, L-shaped metalwork 6, zero-bit photoelectric sensor 7, gate-shaped metal frame 8, detecting position photoelectric sensor 9, white light LED array 10, light
Learn collimating components 11, photosensitive array 12, kit pallet 13, kit 14.Motor 2, displacement platform 3 are arranged in bottom plate 1
Centre, is located on the same line.Motor shaft, which is screwed, is connected with shaft coupling 5, and the other end of shaft coupling is solid by screw
Surely in the rotating shaft for being connected to displacement platform 3.One end in the rotating shaft of displacement platform 3 close to motor 1 is by bridgeware 4, and screw is fixed
Connect kit pallet 13.Multiple kit rest areas are set on kit pallet 13.Each one group of kit rest area correspondence
White light LED array 10.The size of kit rest area is determined according to the size of kit 14, is left on the panel of kit rest area
Multiple circular holes, are distributed in array-like, and the distance between quantity, size and aperture of circular hole are determined by kit 14.Reagent
There is a row dentation protrusion side of box pallet 13, and the number of dentation protrusion is determined by the line number of the aperture in kit 14.Bottom
The side of plate 1 is screwed a L-shaped metalwork 6, is located at the same side with the dentation protrusion of kit pallet 13.L-shaped gold
Some of category part 6 is highly screwed a U-shaped photoelectric sensor, is zero-bit photoelectric sensor 7.Null pick-up 7
Center is centrally located at sustained height with dentation protrusion.The both sides of bottom plate 1 are screwed multiple doorframe shape metal frames 8, door
The line number of the number of frame-shaped metal frame 8 kit rest area on kit pallet 13 is determined.Multiple phases of doorframe shape metal frame 8
It is mutually parallel.The side wall screw of doorframe shape metal frame 8 of neighbouring L-shaped metalwork 6 fixes a U-shaped photoelectric sensor, is detecting position light
Electric transducer 9, detecting position photoelectric sensor 9 is located at sustained height with zero-bit photoelectric sensor 7.The top peace of doorframe shape metalwork
Equipped with white light LED array, optical collimating component.The underface of optical collimating component is photosensitive array.In white light LED array
The columns of LED number aperture in kit 14 determines that the aperture in such as kit 14 has 6 rows 8 to arrange, then one group of white light
LED array is made up of 8 LED.An aperture in each LED correspondences optical collimating component.
When software analysis system sends detection signal, signal transacting control centre sends low frequency pulse signal, P1.0 and sent
High level signal, P1.2 sends low frequency pulse signal, and controlled motor 2 drives kit pallet 13 to be rotated forward compared with low velocity
Detecting position photoelectric sensor 9 is moved to from zero-bit photoelectric sensor 7.Protruded through on kit pallet 13 away from motor side dentation
The center of detecting position photoelectric sensor 9, causes the output level of detecting position photoelectric sensor 9 by high step-down, this level change is logical
Cross pin P1.4 and be transferred to signal transacting control centre, control centre sends instruction, start detection.P4.1 and P4.2 are simultaneously emitted by
Low level signal, enables two line decoders of 3 line -8, and the level combinations sent by P5.1, P5.2, P5.3, one group of enable is white
8 LED in light LED drive circuit, one group of white light LED array of driving are lighted one by one towards same direction, and each LED lights 1
Second, while drive the work of LED corresponding photo-sensitive cells, the transmitted light received is changed into pulse frequency, and by this frequency
Signal passes through P1.1 pins
It is transferred to signal processing control unit.Signal processing control unit has received kit the first row, and last is detected
Data, the first row detection terminates.Signal processing control unit continues low speed and rotated forward, with remote motor side on kit pallet
Dentation protrudes the adjacent center for projecting through detecting position photoelectric sensor.Start to detect the second row.
Whole kit keyhole detection terminates.The time set according to system, repeat above step.By the number detected every time
According to the growth curve for recording and being depicted as bacterium.The drug resistance of bacterium is judged according to growth curve.
It should be noted that herein, such as first and second or the like relational terms are used merely to a reality
Body or operation make a distinction with another entity or operation, and not necessarily require or imply these entities or deposited between operating
In any this actual relation or order.Moreover, term " comprising ", "comprising" or its any other variant are intended to
Nonexcludability is included, so that process, method, article or equipment including a series of key elements not only will including those
Element, but also other key elements including being not expressly set out, or also include being this process, method, article or equipment
Intrinsic key element.In the absence of more restrictions, the key element limited by sentence "including a ...", it is not excluded that
Also there is other identical element in process, method, article or equipment including the key element.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although with reference to the foregoing embodiments
The present invention is described in detail, it will be understood by those within the art that：It still can be to foregoing each implementation
Technical scheme described in example is modified, or carries out equivalent substitution to which part technical characteristic；And these modification or
Replace, the essence of appropriate technical solution is departed from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (8)
1. a kind of detecting system for susceptibility, it is characterised in that：Comprise the following steps：
Step 1: during detection, the LED in white light LED array is lighted and kept for several seconds one by one, LED light by optical collimating component,
By multiple reflections, the stronger light beam of intensity of illumination is formed, is irradiated on the aperture of kit and penetrates the photosensitive battle array of aperture arrival
Row.
Step 2: because antibiotic does not work for the bacterium to the antibiotics resistance, existing to the bacterium of the antibiotics resistance
Bred rapidly during susceptibility, kit aperture, which is mixed with antibiotic and the aperture to the bacterium of the antibiotics resistance, becomes muddy
Turbid, the transmitted light that photosensitive array is detected is weak, then breeds relatively slow to the bacterium of the antibiotic sensitive or even no longer breeds, photosensitive battle array
The transmitted light that row are detected is stronger, and photosensitive array changes into the power of the transmitted light detected the pulse of different frequency, transmission
Give signal transacting control centre.
Step 3: pulse signal is changed into decimal data by signal transacting control centre, and send software analysis system to.
Step 4: software analysis system stores the data received sequentially in time, and timing sends detection signal, detection
During whole susceptibility in aperture liquid turbidity, by the data record detected every time and be depicted as the growth curve of bacterium,
The drug resistance of bacterium is judged according to growth curve.
2. the detecting system according to claim 1 for susceptibility, including it is white light LED array, optical collimating component, photosensitive
Array, signal management control centre and software analysis system, it is characterised in that：Also include telecontrol equipment, photoelectric sensor, LED
Drive circuit and multiplex electronics；The telecontrol equipment includes bottom plate, motor and displacement platform, and motor, displacement platform are installed
Entreat, be located on the same line in the soleplate, motor shaft, which is screwed, is connected with shaft coupling, and the other end of shaft coupling passes through
Screw is fixedly attached in the rotating shaft of displacement platform, and close to motor one end by bridgeware in displacement platform rotating shaft, screw is fixed
Connect and multiple kit rest areas are set on kit pallet, kit pallet, one group of white light of each kit rest area correspondence
LED array, the size of kit rest area is determined according to kit size, and multiple circles are left on the panel of kit rest area
Hole, is distributed in array-like, and the distance between quantity, size and aperture of circular hole are determined by kit, kit pallet
There is a row dentation protrusion side, and the line number of number aperture in kit of dentation protrusion determines that the side of bottom plate passes through spiral shell
Silk fixes a L-shaped metalwork, is located at the same side with the dentation protrusion of kit pallet, some of L-shaped metalwork highly passes through
Screw fixes a U-shaped photoelectric sensor, is zero-bit photoelectric sensor, the center of null pick-up and the centre bit of dentation protrusion
In sustained height, the both sides of bottom plate are screwed multiple doorframe shape metal frames, and the number of doorframe shape metal frame is by kit
The line number of kit rest area is determined on pallet, and multiple doorframe shape metal frames are parallel to each other, the doorframe shape of neighbouring L-shaped metalwork
Metal frame side wall screw fixes a U-shaped photoelectric sensor, is detecting position photoelectric sensor, detecting position photoelectric sensor and zero-bit
Photoelectric sensor is located at sustained height.
3. the detecting system according to claim 2 for susceptibility, it is characterised in that：The top of the doorframe shape metalwork
White light LED array is installed, optical collimating component, the underface of the optical collimating component is photosensitive array.
4. the detecting system according to claim 2 for susceptibility, it is characterised in that：The white light LED array is just white
Light cold light source, colour temperature 6000-7000K, the number of every group of white light LED array determines by the columns of kit, every group of white light LEDs
Array one group of photosensitive array of correspondence.
5. the detecting system according to claim 2 for susceptibility, it is characterised in that：The optical collimating component is metal
Part, inside, which is scratched, multiple cylindrical holes, aperture inner wall smooth, is conducive to the reflection of light, one white light of each aperture correspondence
LED in LED, optical driver circuit control white light LED array is lighted and kept for several seconds one by one.
6. the detecting system according to claim 2 for susceptibility, it is characterised in that：A part of multiplex electronics pass through
Electric signal, which connects signal transacting control centre and LED drive circuit, another part multiplex electronics and connected by electric signal, to be believed
Number processing control centre and photosensitive array, more white light LED array is controlled with the less output end of signal transacting control centre
And photosensitive array.
7. the detecting system according to claim 1 for susceptibility, it is characterised in that：During detection, in signal transacting control
The heart sends signal, and controlled motor protrudes through U-shaped inspection on kit pallet to be rotated forward compared with low velocity away from motor side dentation
The center of location photoelectric sensor, causes the change of optoelectronic switch output level, and this level change is transferred to signal transacting control
Center processed, control centre sends instruction, LED drive circuit drive the LED array on all metal frame tops towards same direction by
Individual to light, the time lighted, drug sensitivity tests were most preferably determined by many experiments, and LED light passes through optical collimating component, by multiple
Reflection, forms the stronger light velocity of illumination and is irradiated to the aperture of kit and penetrates aperture arrival photosensitive array, photosensitive array will connect
The transmitted light received is converted into pulse frequency, and transmitted light is stronger, and frequency is higher, and these frequencies are transferred in signal transacting control
The heart, in signal transacting control centre, these frequencies are converted into decimal data, are transferred to software analysis system, software analysis
System is stored and sorted to data sequentially in time.
8. the detecting system according to claim 1 for susceptibility, it is characterised in that：The signal transacting control centre connects
Harvesting kit the first row, last detects data, and the first row detection terminates, and it is positive that signal processing control unit continues low speed
Rotate, with protruding the adjacent center for projecting through detecting position photoelectric sensor away from motor side dentation on kit pallet, open
Begin the second row of detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710037785.3A CN107064076A (en) | 2017-01-18 | 2017-01-18 | A kind of detecting system for susceptibility |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710037785.3A CN107064076A (en) | 2017-01-18 | 2017-01-18 | A kind of detecting system for susceptibility |
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| CN107064076A true CN107064076A (en) | 2017-08-18 |
Family
ID=59597991
Family Applications (1)
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| CN201710037785.3A Pending CN107064076A (en) | 2017-01-18 | 2017-01-18 | A kind of detecting system for susceptibility |
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| CN2911669Y (en) * | 2006-04-24 | 2007-06-13 | 上海奥普生物医药有限公司 | Fast quantitative medicine sensitivity detecting sheet for mycoplasma |
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| WO2009143446A2 (en) * | 2008-05-23 | 2009-11-26 | The United States Of America, As Represented By The Secretary Of Agriculture | Lambdasa2 endolysin truncation |
| CN103060186A (en) * | 2012-12-24 | 2013-04-24 | 山东省海水养殖研究所 | Aquatic product Edwardsiella tarda rapid drug allergy detection kit |
| CN103760159A (en) * | 2014-01-24 | 2014-04-30 | 山东鑫科生物科技股份有限公司 | Method and system for identifying bacteria and analyzing drug sensitivity |
| CN103820310A (en) * | 2014-02-15 | 2014-05-28 | 江苏中新医药有限公司 | Concentration gradient detection kit for drug sensitivity tests and measuring method thereof |
| CN105136684A (en) * | 2015-08-14 | 2015-12-09 | 上海蓝怡科技股份有限公司 | Multi-sample detection device and method |
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- 2017-01-18 CN CN201710037785.3A patent/CN107064076A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2911669Y (en) * | 2006-04-24 | 2007-06-13 | 上海奥普生物医药有限公司 | Fast quantitative medicine sensitivity detecting sheet for mycoplasma |
| CN201141824Y (en) * | 2007-11-21 | 2008-10-29 | 上海复星佰珞生物技术有限公司 | Turbidimeter for testing bacterium liquid turbidity |
| WO2009143446A2 (en) * | 2008-05-23 | 2009-11-26 | The United States Of America, As Represented By The Secretary Of Agriculture | Lambdasa2 endolysin truncation |
| CN103060186A (en) * | 2012-12-24 | 2013-04-24 | 山东省海水养殖研究所 | Aquatic product Edwardsiella tarda rapid drug allergy detection kit |
| CN103760159A (en) * | 2014-01-24 | 2014-04-30 | 山东鑫科生物科技股份有限公司 | Method and system for identifying bacteria and analyzing drug sensitivity |
| CN103820310A (en) * | 2014-02-15 | 2014-05-28 | 江苏中新医药有限公司 | Concentration gradient detection kit for drug sensitivity tests and measuring method thereof |
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Application publication date: 20170818 |