CN107064044A - The quick determination method and device of polyphenol content in a kind of tea extract - Google Patents
The quick determination method and device of polyphenol content in a kind of tea extract Download PDFInfo
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- CN107064044A CN107064044A CN201710377745.3A CN201710377745A CN107064044A CN 107064044 A CN107064044 A CN 107064044A CN 201710377745 A CN201710377745 A CN 201710377745A CN 107064044 A CN107064044 A CN 107064044A
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- 239000000284 extract Substances 0.000 title claims abstract description 115
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 69
- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 69
- 238000000034 method Methods 0.000 title claims abstract description 26
- 241001122767 Theaceae Species 0.000 title claims abstract 40
- 238000001514 detection method Methods 0.000 claims abstract description 76
- 239000013307 optical fiber Substances 0.000 claims abstract description 73
- 230000005540 biological transmission Effects 0.000 claims abstract description 29
- 238000002329 infrared spectrum Methods 0.000 claims abstract description 29
- 230000003595 spectral effect Effects 0.000 claims abstract description 19
- 238000004611 spectroscopical analysis Methods 0.000 claims abstract description 16
- 238000004458 analytical method Methods 0.000 claims abstract description 14
- 239000000835 fiber Substances 0.000 claims description 26
- 238000001228 spectrum Methods 0.000 claims description 26
- 230000003287 optical effect Effects 0.000 claims description 14
- 229910052736 halogen Inorganic materials 0.000 claims description 10
- 150000002367 halogens Chemical class 0.000 claims description 10
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 claims description 10
- 229910052721 tungsten Inorganic materials 0.000 claims description 10
- 239000010937 tungsten Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 8
- 239000000126 substance Substances 0.000 claims description 8
- 239000003981 vehicle Substances 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 6
- KXGFMDJXCMQABM-UHFFFAOYSA-N 2-methoxy-6-methylphenol Chemical compound [CH]OC1=CC=CC([CH])=C1O KXGFMDJXCMQABM-UHFFFAOYSA-N 0.000 claims description 5
- 229910000530 Gallium indium arsenide Inorganic materials 0.000 claims description 5
- 238000001746 injection moulding Methods 0.000 claims description 5
- 239000004005 microsphere Substances 0.000 claims description 5
- 239000005011 phenolic resin Substances 0.000 claims description 5
- 229920001568 phenolic resin Polymers 0.000 claims description 5
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 3
- 238000002835 absorbance Methods 0.000 claims description 3
- 229910052802 copper Inorganic materials 0.000 claims description 3
- 239000010949 copper Substances 0.000 claims description 3
- 230000004907 flux Effects 0.000 claims description 3
- 238000013433 optimization analysis Methods 0.000 claims description 3
- 238000010223 real-time analysis Methods 0.000 claims description 3
- 230000003044 adaptive effect Effects 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 6
- 238000012545 processing Methods 0.000 abstract description 6
- 238000007705 chemical test Methods 0.000 abstract description 2
- 238000009614 chemical analysis method Methods 0.000 abstract 1
- 238000007405 data analysis Methods 0.000 abstract 1
- 238000004940 physical analysis method Methods 0.000 abstract 1
- 235000013616 tea Nutrition 0.000 description 99
- 244000269722 Thea sinensis Species 0.000 description 96
- 210000003739 neck Anatomy 0.000 description 12
- 239000000243 solution Substances 0.000 description 8
- 235000009569 green tea Nutrition 0.000 description 5
- 238000004445 quantitative analysis Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 3
- 244000062793 Sorghum vulgare Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- RYYVLZVUVIJVGH-UHFFFAOYSA-N caffeine Chemical compound CN1C(=O)N(C)C(=O)C2=C1N=CN2C RYYVLZVUVIJVGH-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 235000019713 millet Nutrition 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- LPHGQDQBBGAPDZ-UHFFFAOYSA-N Isocaffeine Natural products CN1C(=O)N(C)C(=O)C2=C1N(C)C=N2 LPHGQDQBBGAPDZ-UHFFFAOYSA-N 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 229960001948 caffeine Drugs 0.000 description 1
- VJEONQKOZGKCAK-UHFFFAOYSA-N caffeine Natural products CN1C(=O)N(C)C(=O)C2=C1C=CN2C VJEONQKOZGKCAK-UHFFFAOYSA-N 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000013500 data storage Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000005670 electromagnetic radiation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000005064 physico chemical analysis method Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000001028 reflection method Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000002235 transmission spectroscopy Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
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- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention provides the quick determination method and device of polyphenol content in a kind of tea extract.Using conventional physical and chemical analysis method, polyphenol content in tea extract is detected；By the use of half transmitting detection means as the carrier of sample, mini optical fibre device as spectral signal transport vehicle, portable near infrared spectrometer collection tea extract in half transmitting spectroscopic data；By the tea extract polyphenol content and spectroscopic data of collection, with reference to chemometrics method, polyphenol content forecast model in tea extract is set up；By USB transmission, spectroscopic data is real-time transmitted to computer client；With reference to the model program of write-in, in computer client to spectral data analysis, polyphenol content in tea extract is shown in real time.The technology of the present invention, based near infrared spectrum USB transmission technology, quickly quantitative detection can be carried out to polyphenol content in tea extract, compared to traditional Physico-chemical tests, corresponding detection efficiency is remarkably improved, be more suitable for the on-line checking analysis of deep processing field of tea.
Description
Technical field
The present invention relates to the detection technique of polyphenol content in tea extract and equipment, specifically based near infrared spectrum skill
Art, USB transmission technology, realize the method and device of polyphenol content in quantitative analysis tea extract.
Background technology
With the raising developed rapidly with living standards of the people of food industry, the tea beverage mesh represented as health drink
Preceding positive fast development, and as the maximum beverage of the market sales volume in addition to drinking water.Tea Polyphenols (tea polyphenols, TPs)
Be one of topmost functional components in tealeaves, account for the 20%~35% of dry matter of tea amount, determined in tea extract
The bulk composition of flavour, color, is also the critical substances for determining tea leaf quality.Therefore, compel during tea beverage processing
Be essential to be used for quickly detecting and on-line monitoring to polyphenol content in tea extract.
At present, Tea Polyphenols in Tea content is determined using national standard GB/T8313-2008 forint phenolic resin for injection molding mostly, but
This method is time-consuming, consumption chemical reagent, complex steps, it is difficult to adapt to during production of tea drinks the need for detection real-time.
Therefore, it is necessary to go the quick determination method of polyphenol content and the corresponding inspection of exploitation in the accurate quick measure tea extract of research
Measurement equipment, this has highly important meaning for deep processing field of tea.
Near infrared spectrum is one kind between visible ray (visible light, VIS) and mid-infrared light (middle-
Infrared, MIR) between electromagnetic radiation as waves, corresponding wave-length coverage be 780~2500nm, the Tea Polyphenols in for dry tea
The Fast nondestructive evaluation of content and the Qualitive test of tealeaves obtain more satisfactory result, and nearly infrared application is in tea
The also rare report of the detection of composition in extract solution；Analysis and research simultaneously qualitative and quantitative on tealeaves, are confined to experiment mostly
Fourier's near-infrared (FT-NIR) detection of room operation, and corresponding instrument price is expensive, on line real time is difficult to.
Such as " a kind of device of rapidly and nondestructively detecting content of green tea composition that Chinese Patent Application No. is 200810162600.2
And method " patent, green tea absorption spectrum is determined using diffusing reflection method, with reference to chemometrics method, realized in green tea
Amino acid, caffeine, Tea Polyphenols, the Fast nondestructive evaluation of four kinds of components of moisture；But the device can not be in tea extract
Internal component realizes quick determine, it is impossible to be used in the Site Detection of deep processing field of tea.
Such as Jiang Liyi, Liu Fuli et al., in " the light path selection of Tea Polyphenols Near-Infrared Quantitative Analysis in a green tea millet paste " text
In, modeling analysis, but the research have been carried out to the NIR transmittance spectroscopy in green tea millet paste using offset minimum binary modeling method
The MPA Fourier Transform Near Infrared instruments of the German Brooker optical instrument company used, expensive, volume is larger,
Do not have stronger universality for deep processing field of tea.
Therefore the method for finding polyphenol content in a kind of easy quick detection tea extract, to meeting deep processing field of tea
There is important realistic meaning.The present invention is made by the use of half transmitting detection means as the carrier of spectra collection, mini optical fibre device
For the transport vehicle of spectral signal, portable near infrared spectrometer gathers the half transmitting spectroscopic data in tea extract, with reference to normal
Rule physico-chemical analysis method obtains polyphenol content in different grades of tea extract；By polyphenol content and tea in the tea extract of collection
Half transmitting spectroscopic data in extract solution, with reference to chemometrics method, sets up polyphenol content forecast model in tea extract；It is based on
USB transmission technology, the quantitative analysis of polyphenol content in tea extract can be realized in computer end.
The content of the invention
It is an object of the invention to provide the quick determination method and device of polyphenol content in a kind of tea extract, realization pair
The fast quantitative analysis of polyphenol content in tea extract.
For the method and device of the present invention, the technical scheme specifically used is as follows：
The quick determination method of polyphenol content in a kind of tea extract, it is characterised in that comprise the following steps：
Step one, in tea extract polyphenol content detection：Using forint phenolic resin for injection molding, different grades of tea is determined
Polyphenol content in extract solution, obtains polyphenol content in the tea extract of physico-chemical analysis measure；
Step 2, the collection of tea extract spectroscopic data：Using light source module as signal source, half transmitting detection means is made
Carried for the carrier of sample, mini optical fibre device as transport vehicle, the portable near infrared spectrum module reception tea of spectral signal
The half transmitting spectrum in liquid is taken, spectra collection in the tea extract of quick nondestructive is carried out, obtains half transmitting in tea extract closely red
External spectrum data；
Step 3, the optimization analysis of tea extract detection model：SNV is converted in tea extract using standard normal variable
Half transmitting near infrared spectrum data is pre-processed, then the tea that pretreated near infrared spectrum data and physico-chemical analysis are determined
Polyphenol content in extract solution, sets up tea extract detection model；
The quick detection of polyphenol content in step 4, tea extract：Computer client is connected to by USB data line, it is right
Near infrared spectrum data real-time Transmission in tea extract；The tea extract detection model write with reference to exploitation software, in software circle
The sample spectrum information of tea extract is analyzed at face end in real time, realizes the quick detection of polyphenol content in tea extract；.
Further, in step 2, the light source module has two halogen tungsten lamps；The halogen tungsten lamp operating power is 1.4W,
Placed in 40 ° of angles of level；It is described portable, be that near infrared spectrometer is integrated into 10*6*5cm cuboid, can one hand take
Band；It is described quick for scanning used time 2s/ times.
Further, in step 2, the wave-length coverage of the near infrared spectrum module is 900~1700nm, can effectively be covered
Near infrared spectrum region；The slit size of the near infrared spectrum module is 1.69*0.025mm, and signal to noise ratio is 6000:1, have
High flux spectral information；The near infrared spectrum module, using InGaAs detectors, with higher sensitivity and detectivity.
Further, in step 2, in the half transmitting detection means, spectra collection is carried out in half transmitting mode；Described half
Transmission mode, is the optical signal for only receiving single halogen tungsten lamp；The optical signal, incidence end is gathered in through special ball lens
Interface.
Further, in step 2, in the mini optical fibre device, optical signal is passed from fiber exit port through mini optical fibre
Transport to fiber exit end；It is semi-transparent in the fiber exit end, the tea extract that optical signal is mapped in cuvette neck to be incident upon just
Take formula spectrometer and receive half transmitting signal.
Further, in step 3, the tea extract detection model joint [1024.31nm 1081.22nm],
[1311.08nm 1362.62nm], [1518.36nm 1563.60nm], [1612.88nm 1656.14nm] four range of wavelengths
It is interior.
Further, in step 4, the detection model of multiple internal component indexs in tea extract is supported in the software write-in；
The tea extract detection model, with the increase of collecting sample number, the spectral information increase in characteristic wavelength interval, model can be with
Voluntarily optimize；In real time analysis, using superposition spectrum by the way of verify tea extract spectral intensity, absorbance and transmissivity it is steady
It is qualitative.
The technical scheme of device of the present invention is：
The detection means of polyphenol content in a kind of tea extract, it is characterised in that including half transmitting detection means and miniature
Fiber device；
The half transmitting detection means includes incident end interface, light source exit portal, cuvette neck, light source incidence mouthful, spiral shell
Hole；Device front end is provided with light source exit portal, and rear end is provided with light source incidence mouthful, screw, and device upper end is provided with cuvette neck, device
Side is provided with incident end interface；
The half transmitting detection means is fixed through screw with portable spectrometer；The light signal is poly- through light source incidence mouthful
The ball lens combined in inside device；The ball lens are placed in incident end interface；The incident end interface is connected optical fiber and entered
Penetrate end；The light source exit portal is through ball lens focus in cuvette outer wall；The cuvette is placed in cuvette neck；
The mini optical fibre device includes optical fiber transmission port, optical fiber incidence end, mini optical fibre, fiber exit end；Optical fiber
Incidence end center is provided with optical fiber transmission port；The optical fiber incidence end is connected with fiber exit end through mini optical fibre；The light
Fine exit end is connected with light source exit portal.
Further, in the half transmitting detection means, the screw through 2* Φ 2mm is anchored on portable spectrometer；It is described small
The a diameter of Φ 10mm of globe lens, its focus can effectively be placed in optical fiber transmission port；The light source exit portal is the SMA905 of standard
Interface, with preferable universality；The cuvette neck specification 12.5*12.5*45mm, is applicable the quartzy standard colorimetrics of 10mm
Ware.
Further, in the mini optical fibre device, optical fiber incidence end is bottom surface circular diameter Φ 10mm, a height of copper materials of 10mm
The cylinder of material, light weight is portable；The mini optical fibre length is 105mm, is connected to optical fiber incidence end and fiber exit end,
Optical fiber incidence end center is provided with Φ 2mm optical fiber transmission port.
Beneficial effects of the present invention, it is specific as follows：
First, for the collection of tea extract spectroscopic data：The present invention is used as spectrum using half transmitting spectrum detection device
The carrier of collection, mini optical fibre device are extracted as the transport vehicle of spectral signal with reference to portable near infrared spectrometer collection tea
Half transmitting spectroscopic data in liquid, with the advantage such as quick, lossless, portable, meets the requirement of Site Detection analysis；
Second, for the transmission of spectroscopic data in tea extract：The present invention uses USB transmission technology, carries out half transmitting light
The transmission of modal data, signal stabilization, noise is small, beneficial to effective transmission of spectroscopic data：
Third, for the quick detection of polyphenol content in tea extract：The present invention is directed to polyphenol content in tea extract, builds
Corresponding forecast model is found, is analyzed different from conventional Physico-chemical tests, quick determination method is more easy, and operability is higher；Branch
Internal multicomponent prediction in tea extract is held, and with data storage function, polyphenol content mould in later stage tea extract can be improved
The robustness of type；
Fourth, the optimization for polyphenol content detection model in tea extract is analyzed：For polyphenol content in tea extract
Detection model is in joint [1024.31nm 1081.22nm], [1311.08nm 1362.62nm], [1518.36nm
1563.60nm], [1612.88nm 1656.14nm] four range of wavelengths when, model coefficient correlation is higher, and stability is preferable；
Fifth, for the detection means of polyphenol content in tea extract：The present invention is used as spectrum using half transmitting detection means
The carrier of collection, mini optical fibre device are as the transport vehicle of spectral signal, and combined type portable near infrared spectrometer collection tea carries
The half transmitting spectroscopic data in liquid is taken, quickly quantitative detection can be carried out to polyphenol content in tea extract, it is adaptable to which scene is fast
Speed detection and analysis.
Brief description of the drawings
The schematic device of Fig. 1 present invention；Fig. 1 (a) is half transmitting detection means schematic diagram of the present invention；Fig. 1 (b) is this
Invention mini optical fibre schematic device；
Fig. 2 is the light path principle figure of polyphenol content detection means in tea extract of the present invention；
In figure：1 incident end interface, 2 light source exit portals, 3 cuvette necks, 4 light source incidences mouthful, 5 screws, the transmission of 6 optical fiber
Port, 7 optical fiber incidence ends, 8 mini optical fibres, 9 fiber exit ends；
Embodiment
Technical scheme is described in further detail below with reference to the drawings and specific embodiments.The present invention
Solution internal component is quantitatively detected with versatility.Target polyphenol content selected by the implementation case is middle master in tea extract
Composition is wanted, other solution internal components refer to the progress of this example.
Carrier of the implementation case using half transmitting detection means as half transmitting spectra collection, is made using mini optical fibre device
For the transport vehicle of spectral signal；Combined type portable near infrared spectrometer, detects half transmitting spectral signal, reaches fast quantification point
The purpose of analysis.Concrete technical scheme is as follows：
Embodiments of the invention are described below in detail, the example of the embodiment is shown in the drawings, wherein from beginning to end
Same or similar label represents same or similar element or the element with same or like function.Below with reference to attached
The embodiment of figure description is exemplary, is only used for explaining the present invention, and is not considered as limiting the invention.
Step one, in tea extract polyphenol content detection：Using forint phenolic resin for injection molding, different grades of tea is determined
Polyphenol content in extract solution, obtains polyphenol content in the tea extract of physico-chemical analysis measure；
Step 2, the collection of tea extract spectroscopic data：Using light source module as signal source, half transmitting detection means is made
Carried for the carrier of sample, mini optical fibre device as transport vehicle, the portable near infrared spectrum module reception tea of spectral signal
The half transmitting spectrum in liquid is taken, spectra collection in the tea extract of quick nondestructive is carried out, obtains half transmitting in tea extract closely red
External spectrum data；In step 2, the light source module has two halogen tungsten lamps；The halogen tungsten lamp operating power is 1.4W, in water
Flat 40 ° of angles are placed；It is described portable, it is that near infrared spectrometer is integrated into 10*6*5cm cuboid, singlehanded can carries；Institute
State quick for scanning used time 2s/ times.
The wave-length coverage of the near infrared spectrum module is 900~1700nm, can effectively cover near infrared spectrum region；Institute
The slit size for stating near infrared spectrum module is 1.69*0.025mm, and signal to noise ratio is 6000:1, with high flux spectral information；Institute
Near infrared spectrum module is stated, using InGaAs detectors, with higher sensitivity and detectivity.
In the half transmitting detection means, spectra collection is carried out in half transmitting mode；The half transmitting mode, is only to connect
Receive the optical signal of single halogen tungsten lamp；The optical signal, incident end interface 1 is gathered in through special ball lens.
In the mini optical fibre device, optical signal is transmitted to fiber exit end from fiber exit port 6 through mini optical fibre 8
9；The semi-transparent portable spectrometer that is incident upon connects in the fiber exit end 9, the tea extract that optical signal is mapped in cuvette neck 3
Receive half transmitting signal.
Step 3, the optimization analysis of tea extract detection model：SNV is converted in tea extract using standard normal variable
Half transmitting near infrared spectrum data is pre-processed, then the tea that pretreated near infrared spectrum data and physico-chemical analysis are determined
Polyphenol content in extract solution, sets up tea extract detection model；The tea extract detection model is in joint
[1024.31nm1081.22nm]、[1311.08nm 1362.62nm]、[1518.36nm 1563.60nm]、
In [1612.88nm1656.14nm] four range of wavelengths.
The quick detection of polyphenol content in step 4, tea extract：Computer client is connected to by USB data line, it is right
Near infrared spectrum data real-time Transmission in tea extract；The tea extract detection model write with reference to exploitation software, in software circle
The sample spectrum information of tea extract is analyzed at face end in real time, realizes the quick detection of polyphenol content in tea extract.The software
The detection model of multiple internal component indexs in tea extract is supported in write-in；The tea extract detection model, with collecting sample
Spectral information increase in several increases, characteristic wavelength interval, model can voluntarily optimize；The real-time analysis, using superposition
The mode of spectrum verifies the stability of the spectral intensity, absorbance and transmissivity of tea extract.
The detection means of polyphenol content in a kind of tea extract, including half transmitting detection means and mini optical fibre device；
The half transmitting detection means includes incident end interface 1, light source exit portal 2, cuvette neck 3, light source incidence mouthful
4th, screw 5；Device front end is provided with light source exit portal 2, and rear end is provided with light source incidence mouthful 4, screw 5, and device upper end is provided with cuvette
Neck 3, device side is provided with incident end interface 1；
The half transmitting detection means is fixed through screw 5 and portable spectrometer；The light signal is through light source incidence mouthful 4
It is gathered in the ball lens inside device；The ball lens are placed in incident end interface 1；The incident end interface 1 connects light
Fine incidence end 7；The light source exit portal 2 is through ball lens focus in cuvette outer wall；The cuvette is placed in cuvette neck 3
It is interior；
The mini optical fibre device includes optical fiber transmission port 6, optical fiber incidence end 7, mini optical fibre 8, fiber exit end 9；
The center of optical fiber incidence end 7 is provided with optical fiber transmission port 6；The optical fiber incidence end 7 is with fiber exit end 9 through the phase of mini optical fibre 8
Even；The fiber exit end 9 is connected with light source exit portal 2.
In the half transmitting detection means, the screw through 2* Φ 2mm is anchored on portable spectrometer；The microsphere lenses are straight
Footpath is Φ 10mm, and its focus can effectively be placed in optical fiber transmission port 6；The light source exit portal 2 is the SMA905 interfaces of standard, tool
There is preferable universality；The cuvette neck specification 12.5*12.5*45mm, is applicable the quartzy standard colorimetric wares of 10mm.
In the mini optical fibre device, optical fiber incidence end 7 is bottom surface circular diameter Φ 10mm, the circle of a height of 10mm copper materials
Post, light weight is portable；The length of mini optical fibre 8 is 105mm, is connected to optical fiber incidence end 7 and fiber exit end 9, optical fiber
The center of incidence end 7 is provided with Φ 2mm optical fiber transmission port 6.
The half transmitting detection means schematic diagram of Fig. 1 present invention, according to Fig. 1 (a), the half transmitting detection means, through screw
Fixed with portable spectrometer；The light signal is gathered in microsphere lenses through light source incidence mouthful；The microsphere lenses be placed in into
Penetrate in end interface；The incident end interface, connects optical fiber incidence end；The light source exit portal focuses on cuvette through microsphere lenses
Outer wall；The cuvette is placed in cuvette neck；
Fig. 1 (b) for the present invention mini optical fibre schematic device, in the mini optical fibre device, optical fiber incidence end center
Place is provided with optical fiber transmission port；The optical fiber incidence end is connected with fiber exit end through mini optical fibre；The fiber exit end with
Light source exit portal is connected.
Below in conjunction with specific case study on implementation, the present invention will be described in detail.But these case study on implementation are not intended to limit this
Invention, structure that one of ordinary skill in the art is made according to these case study on implementation, method or conversion functionally are wrapped
Containing within the scope of the present invention.
Case study on implementation：The detection of polyphenol content in different brackets tea extract
The quick determination method of polyphenol content, comprises the following steps in a kind of new tea extract：
(1) 60 kinds of different grades of tea extract samples are taken, using forint phenolic resin for injection molding, detect many in different samples
Phenol content.
(2) according to operating procedure, connection near infrared spectrometer, half transmitting detection means and mini optical fibre device；Each not
Sample in the tea extract of ad eundem, takes three parallel test samples respectively.Spectrometer relevant parameter, is set to：Scanning wavelength
Scope 900nm-1700nm, resolution ratio 10.53, number of scan points 400, smoothness 5 is counted, mean scan number of times 5 times, is carried out near red
Outer half transmitting spectroscopic data detection.
(3) near infrared spectrum of collection contains noise information, background drift etc., is converted (SNV) using standard normal variable
Spectroscopic data is pre-processed；Selection variables are carried out using the interval PLS (si-PLS) of joint, and to spectrum number
According to being modeled, in quantitative analysis tea extract in polyphenol content.When principal component is 9, joint [3 8 12 14] is interval, its
Coefficient correlation reaches that 0.965, RMSECV is 1.36, and model stability is preferable.Table 1 extracts for the tea based on near-infrared spectrum technique
Polyphenol content predicts the outcome and physico-chemical analysis result in liquid.
Table 1
| Sample number | Measured value (%) | Predicted value (%) | Error |
| 1 | 36.42 | 35.89 | -0.53 |
| 2 | 37.55 | 36.51 | -1.04 |
| 3 | 38.28 | 37.66 | -0.62 |
| 4 | 42.22 | 44.12 | 1.90 |
| 5 | 46.15 | 47.89 | 1.74 |
| 6 | 50.35 | 51.58 | 1.23 |
| 7 | 53.22 | 52.14 | -1.08 |
| 8 | 57.68 | 58.86 | 1.18 |
It is illustrated in figure 2 Fig. 1 light path principle figure, half transmitting module：Halogen tungsten lamp is poly- through ball lens as light signal
Jiao, in transmission mode through cuvette, incites somebody to action in mini optical fibre input, then through light transmission fiber source signal to fiber-optic output
Transmitted spectrum signal is transmitted to portable spectrometer.
Spectrometer：The transmitted spectrum signal of sample passes through scanning slit, after collimated lens calibration, points to diffraction grating；
Line focus lens, light is got on the corresponding DLP micro mirrors mirror row of special wavelength light again；Last acquired camera lens capture, will
Light points to InGaAs detectors, on ADC analog-digital converters, obtains the half transmitting spectroscopic data of sample.
Transmission advantage：
Spectrometer based on DLP instead of conventional line array using digital micro-mirror (DMD) and single-point InGaAs detectors
Detector.The one group mirror row corresponding with special wavelength light by opening in order, correspondence light is pointed at detector, and is caught
Obtain.Arranged by scanning one group of mirror on DMD, transmitted spectrum can be calculated.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " illustrative examples ",
The description of " example ", " specific example " or " some examples " etc. means to combine specific features, the knot that the embodiment or example are described
Structure, material or feature are contained at least one embodiment of the present invention or example.In this manual, to above-mentioned term
Schematic representation is not necessarily referring to identical embodiment or example.Moreover, specific features, structure, material or the spy of description
Point can in an appropriate manner be combined in any one or more embodiments or example.
Although an embodiment of the present invention has been shown and described, it will be understood by those skilled in the art that：Not
In the case of departing from the principle and objective of the present invention a variety of change, modification, replacement and modification can be carried out to these embodiments, this
The scope of invention is limited by claim and its equivalent.
Claims (10)
1. the quick determination method of polyphenol content in a kind of tea extract, it is characterised in that comprise the following steps：
Step one, in tea extract polyphenol content detection：Using forint phenolic resin for injection molding, determine different grades of tea and extract
Polyphenol content in liquid, obtains polyphenol content in the tea extract of physico-chemical analysis measure；
Step 2, the collection of tea extract spectroscopic data：Using light source module as signal source, half transmitting detection means is used as sample
Transport vehicle, portable near infrared spectrum module reception tea extract of the carrier, mini optical fibre device of product as spectral signal
In half transmitting spectrum, carry out quick nondestructive tea extract in spectra collection, obtain half transmitting near infrared light in tea extract
Modal data；
Step 3, the optimization analysis of tea extract detection model：SNV is converted to semi-transparent in tea extract using standard normal variable
Near infrared spectrum data is penetrated to be pre-processed, then the tea that pretreated near infrared spectrum data and physico-chemical analysis are determined is extracted
Polyphenol content in liquid, sets up tea extract detection model；
The quick detection of polyphenol content in step 4, tea extract：Computer client is connected to by USB data line, tea is carried
Take near infrared spectrum data real-time Transmission in liquid；The tea extract detection model write with reference to exploitation software, at software interface end
The sample spectrum information of analysis tea extract, realizes the quick detection of polyphenol content in tea extract in real time.
2. the quick determination method of polyphenol content in a kind of tea extract according to claim 1, it is characterised in that step
In two, the light source module has two halogen tungsten lamps；The halogen tungsten lamp operating power is 1.4W, is placed in 40 ° of angles of level；Institute
State portable, be that near infrared spectrometer is integrated into 10*6*5cm cuboid, singlehanded can carry；It is described quick for the scanning used time
2s/ times.
3. the quick determination method of polyphenol content in a kind of tea extract according to claim 1, it is characterised in that step
In two, the wave-length coverage of the near infrared spectrum module is 900~1700nm, can effectively cover near infrared spectrum region；It is described
The slit size of near infrared spectrum module is 1.69*0.025mm, and signal to noise ratio is 6000:1, with high flux spectral information；It is described
Near infrared spectrum module uses InGaAs detectors.
4. the quick determination method of polyphenol content in a kind of tea extract according to claim 1, it is characterised in that step
In two, in the half transmitting detection means, spectra collection is carried out in half transmitting mode；The half transmitting mode, is only to receive
The optical signal of single halogen tungsten lamp；The optical signal, incident end interface (1) is gathered in through special ball lens.
5. the quick determination method of polyphenol content in a kind of tea extract according to claim 1, it is characterised in that step
In two, in the mini optical fibre device, optical signal is transmitted to fiber exit end from fiber exit port (6) through mini optical fibre (8)
(9)；It is semi-transparent in the fiber exit end (9), the tea extract that optical signal is mapped in cuvette neck (3) to be incident upon portable light
Spectrometer receives half transmitting signal.
6. the quick determination method of polyphenol content in a kind of tea extract according to claim 1, it is characterised in that step
In three, the tea extract detection model joint [1024.31nm 1081.22nm], [1311.08nm 1362.62nm],
In four range of wavelengths of [1518.36nm 1563.60nm], [1612.88nm 1656.14nm].
7. the quick determination method of polyphenol content in a kind of tea extract according to claim 1, it is characterised in that step
In four, the detection model of multiple internal component indexs in tea extract is supported in the software write-in；The tea extract detects mould
Type, with the increase of collecting sample number, the spectral information in characteristic wavelength interval increases, and model can voluntarily optimize；It is described real-time
Analysis, verifies the stability of the spectral intensity, absorbance and transmissivity of tea extract by the way of superposition spectrum.
8. the detection means of polyphenol content in a kind of tea extract, it is characterised in that including half transmitting detection means and miniature light
Fine device；
The half transmitting detection means includes incident end interface (1), light source exit portal (2), cuvette neck (3), light source incidence
Mouth (4), screw (5)；Device front end is provided with light source exit portal (2), and rear end is provided with light source incidence mouthful (4), screw (5), device
End is provided with cuvette neck (3), and device side is provided with incident end interface (1)；
The half transmitting detection means is fixed through screw (5) with portable spectrometer；The light signal is through light source incidence mouthful (4)
It is gathered in the ball lens inside device；The ball lens are placed in incident end interface (1)；The incident end interface (1) connects
Logical optical fiber incidence end (7)；The light source exit portal (2) is through ball lens focus in cuvette outer wall；The cuvette is placed in ratio
In color ware neck (3)；
The mini optical fibre device includes optical fiber transmission port (6), optical fiber incidence end (7), mini optical fibre (8), fiber exit end
(9)；Optical fiber incidence end (7) center is provided with optical fiber transmission port (6)；The optical fiber incidence end (7) passes through with fiber exit end (9)
Mini optical fibre (8) is connected；The fiber exit end (9) is connected with light source exit portal (2).
9. the detection means of polyphenol content in a kind of tea extract according to claim 8, it is characterised in that described semi-transparent
Penetrate in detection means, the screw through 2* Φ 2mm is anchored on portable spectrometer；The a diameter of Φ 10mm of microsphere lenses, it is burnt
Point can effectively be placed in optical fiber transmission port (6)；The light source exit portal (2) is the SMA905 interfaces of standard, with preferably general
Adaptive；The cuvette neck specification 12.5*12.5*45mm, is applicable the quartzy standard colorimetric wares of 10mm.
10. the detection means of polyphenol content in a kind of tea extract according to claim 8, it is characterised in that described micro-
In type fiber device, optical fiber incidence end (7) be bottom surface circular diameter Φ 10mm, the cylinder of a height of 10mm copper materials, light weight, easily
Carry；Mini optical fibre (8) length is 105mm, is connected to optical fiber incidence end (7) and fiber exit end (9), optical fiber incidence end
(7) center is provided with Φ 2mm optical fiber transmission port (6).
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108241016A (en) * | 2018-02-01 | 2018-07-03 | 江苏大学 | The rapid detection method and device of theaflavin content in a kind of black tea |
| CN108572158A (en) * | 2018-07-11 | 2018-09-25 | 天津博霆光电技术有限公司 | A kind of household superminiature near infrared spectrum tester |
| CN109187454A (en) * | 2018-08-10 | 2019-01-11 | 江苏大学 | A kind of fluorescence detection method of tealeaves risk substance fluorine |
| CN110161025A (en) * | 2019-06-12 | 2019-08-23 | 郑州轻工业学院 | A kind of Continuous Flow Analysis method measuring tea polyphenols in tealeaves and tea product |
| CN111024630A (en) * | 2019-11-20 | 2020-04-17 | 大连民族大学 | Portable visible absorption spectrometer |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08114543A (en) * | 1994-10-17 | 1996-05-07 | Shizuoka Seiki Co Ltd | Method for evaluating quality of leaf tea |
| CN1811391A (en) * | 2006-02-22 | 2006-08-02 | 中国农业大学 | Quantitative detecting method for the polyphenol content |
| CN101059426A (en) * | 2007-05-29 | 2007-10-24 | 浙江大学 | Method for non-destructive measurement for tea polyphenol content of tea based on near infrared spectrum technology |
| CN103743703A (en) * | 2013-12-20 | 2014-04-23 | 贵州省分析测试研究院 | Method for detecting main components in tea leaves by adopting near infrared spectrum |
-
2017
- 2017-05-25 CN CN201710377745.3A patent/CN107064044B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08114543A (en) * | 1994-10-17 | 1996-05-07 | Shizuoka Seiki Co Ltd | Method for evaluating quality of leaf tea |
| CN1811391A (en) * | 2006-02-22 | 2006-08-02 | 中国农业大学 | Quantitative detecting method for the polyphenol content |
| CN101059426A (en) * | 2007-05-29 | 2007-10-24 | 浙江大学 | Method for non-destructive measurement for tea polyphenol content of tea based on near infrared spectrum technology |
| CN103743703A (en) * | 2013-12-20 | 2014-04-23 | 贵州省分析测试研究院 | Method for detecting main components in tea leaves by adopting near infrared spectrum |
Non-Patent Citations (2)
| Title |
|---|
| 吴瑞梅 等: "特征变量筛选在近红外光谱测定绿茶汤中茶多酚的应用", 《农业机械学报》 * |
| 许文丽 等: "脐橙放置方位对近红外光谱检测结果的影响", 《光谱学与光谱分析》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108241016A (en) * | 2018-02-01 | 2018-07-03 | 江苏大学 | The rapid detection method and device of theaflavin content in a kind of black tea |
| CN108241016B (en) * | 2018-02-01 | 2020-08-28 | 江苏大学 | Method and device for rapidly detecting theaflavin content in black tea |
| CN108572158A (en) * | 2018-07-11 | 2018-09-25 | 天津博霆光电技术有限公司 | A kind of household superminiature near infrared spectrum tester |
| CN109187454A (en) * | 2018-08-10 | 2019-01-11 | 江苏大学 | A kind of fluorescence detection method of tealeaves risk substance fluorine |
| CN110161025A (en) * | 2019-06-12 | 2019-08-23 | 郑州轻工业学院 | A kind of Continuous Flow Analysis method measuring tea polyphenols in tealeaves and tea product |
| CN111024630A (en) * | 2019-11-20 | 2020-04-17 | 大连民族大学 | Portable visible absorption spectrometer |
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Application publication date: 20170818 Assignee: NANJING RONGDIAN FOODSTUFF Co.,Ltd. Assignor: JIANGSU University Contract record no.: X2020980010180 Denomination of invention: A rapid detection method and device for polyphenol content in tea extract Granted publication date: 20200925 License type: Exclusive License Record date: 20201230 |
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