CN107056353A - A kind of method that utilization cheap aquatic product prepares microorganism compost - Google Patents
A kind of method that utilization cheap aquatic product prepares microorganism compost Download PDFInfo
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- CN107056353A CN107056353A CN201710427492.6A CN201710427492A CN107056353A CN 107056353 A CN107056353 A CN 107056353A CN 201710427492 A CN201710427492 A CN 201710427492A CN 107056353 A CN107056353 A CN 107056353A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical Kinetics & Catalysis (AREA)
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Abstract
A kind of method that utilization cheap aquatic product prepares microorganism compost, is with 4 by cheap aquatic product and rice husk meal matrix：1 amount carries out the preparation of heap body, and it is respectively Bacillus cercus, Alcaligenes faecalis, Arthrobacter protophormiae and Proteus penneri with bacterium to filter out fermentation；Optimize composite bacteria and expand condition of culture：Temperature is 30 DEG C, and PH=6, rotating speed is 160r/min, and inoculum concentration is 5%, and culture medium most preferably culture is sucrose 20g/l, yeast extract 30g/l, magnesium sulfate 0.15g/l, sodium chloride 0.13g/l, potassium dihydrogen phosphate 0.13g/l.The nutrient content for obtaining compost finished product is：Amino acid nitrogen content Wei≤0.5% of compost fermentation finished product, soluble Dan white matters Han Liang≤200mg/g You machine matter Han Liang≤40%, total nutrient Han Liang≤4%, induced worm egg death rate, coliform group count, arsenic, cadmium, lead, chromium, mercury and mercuric compounds content reach Standard.Nutrient content in fertilizer is improved, the twice laid of low value water product resource is realized.
Description
Technical field
The present invention relates to a kind of method of microorganism compost, particularly a kind of cheap aquatic product fermentation prepares microorganism compost
Method.
Background technology
Compost is exactly under conditions of certain temperature, humidity and PH, to make discarded object using obligate or facultative well foster microorganism
In fresh organic matter occur biochemical degradation, organic matter is converted into the process of stable humus by labile state.Have
After the degraded of machine thing the humus that produces have improved soil, promote fertilizer efficiency, regulating crop growth, the resistance for improving crop and
Improve the functions such as crop quality.Therefore composting process can be used as one of important method of Stock raising waste harmless treatment.
It is existing to prepare in compost method mostly using the external strain of purchase, and the fermentation maturity phase is between 25-30 days.This reality
The separation screening for the different times progress superior microorganism that fermented from heap body is tested, antagonism between microorganism is reduced to greatest extent, plus
Become thoroughly decomposed soon speed.
With developing rapidly for fish processing industry, the amount of fishing of aquatic products also increases sharply, due to some small volumes, body
The collection of the long small fish shrimp in 2-3cm or so and difficulty of processing are too big, therefore are often discarded in seashore.Research data shows,
These chemical compositions for fishing for discarded cheap aquatic product are that moisture accounts for 70-80%, and crude protein accounts for 20% or so, and fat accounts for 6.5-30%,
Below carbohydrate 1%, ash content 1-2%, and its component content is different with the species of fish, volume size, fishes and shrimps ratio difference, if directly
It is discarded by the sea with waste formation, stink, attracting flies winged insect etc. are had after rotting, great environmental pollution is caused.And no
Economic benefit beneficial to enterprise is improved.If can make full use of, the added value of fish processing is not only improved, while environment can be reduced
Pollution.
The content of the invention
It is an object of the invention to provide a kind of method that utilization cheap aquatic product fermentation prepares microorganism compost, by low value
Aquatic products carry out Composting treatment, carry out twice laid.The problem of solving difficult cheap aquatic product processing, pollution environment simultaneously, hair
Ferment, which turns into, can solve that soil is hardened caused by chemical fertilizer and chemical fertilizer nutrient Single-issue after compost, improve soil
Organic quality.
The method that a kind of utilization cheap aquatic product that the present invention is provided prepares microorganism compost, by cheap aquatic product and rice chaff
After powder mixing, regulation heap body carbon-nitrogen ratio is in alkalescent in 50-60%, PH in 30-50%, water content, compost is set up, in compost fermentation
1st, 3,5,7,15, during 21 days, extract superior microorganism carry out it is compound expand culture, for preparing microbial bacterial agent；By bacterium
Agent is mixed with cheap aquatic product raw material, prepares compost, is treated its fermentation maturity drying and processing under the conditions of 105 DEG C, that is, is obtained heap
Fertile finished product；The notable feature of compost fermentation finished product is solubility Dan white matters Han Liang≤200mg/g.
The inventive method comprises the following steps：
（1）Feedstock capture：The cheap aquatic product that seashore is adopted back carries out rubbing processing, uses rice husk meal for fermentation substrate, both
It is 4 to add part by weight：1；
（2）Fermentation extracts from compost fermentation body with strain, is respectively Bacillus cercus (bacillus through strain idenfication
Cereus.frankland), Alcaligenes faecalis (Alcaligenes faecalis), Arthrobacter protophormiae (Arthrobacter), Peng
Family name mycetozoan (Proteus penneri)；
（3）When preparing mixed bacteria liquid activating solution, the inoculum concentration of 4 kinds of bacterium is respectively every kind of 1 ring；The optimum temperature for expanding culture is 30
DEG C, PH is 6, and shaking table culture 12h, rotating speed is 160r/min；The bacterium amount that connects of mixed bacteria liquid is 5%（v/v）, optimal medium is sugarcane
Sugared 20g/l, yeast extract 30g/l, magnesium sulfate 0.15g/l, sodium chloride 0.13g/l, potassium dihydrogen phosphate 0.13g/l；
（4）The preparation of fermenting agent：Composite bacteria scale-up medium 500ml is mixed with 50g rice husk meals, supplement 20g Portugals
Dried and both obtained after grape sugar, fermentation 24h；
（5）Compost fermentation：It regard the composite fermentation microbial inoculum obtained in step (4) as fermentation substrate and the low-value aquatic product for grinding processing
Product carry out mixed fermentation, and fermentation period is 10-20 days；
（6）It is prepared by compost finished product：After fermentation ends, obtained finished product is dried, crushed and packed, low-value aquatic product is both obtained
Product compost finished product.
Embodiment
The present invention is described further with reference to embodiments, but protection scope of the present invention not limited to this.
Embodiment 1
The cheap aquatic product that 15 jin of seashores are adopted back, including discarded low value trash fish shrimp is fished for, the shellfish on bank is retained in after ebb
Class fishes and shrimps etc. are standby after being rubbed；3 jin of rice husk meals are placed in autoclave after crossing 20 mesh sieves, set 121 DEG C of sterilizings of sterilising conditions
Handle 20min.Two kinds of raw materials are mixed, regulation water content is 56.34%, PH=7.34, carries out the foundation of compost model, is placed in 15-
Fermented and cultured under the conditions of 25 DEG C.In fermentation 1, during 3,5,7,15,21 days, microbiologic population's group in separately sampled determination sample
Into, the extraction of superior microorganism in heap body is carried out by the method for microorganism plate count,（Extraction is that nutrient agar is trained with culture
Support base and casein medium）, then separated and purified using plate streak.4 plants of bacterium point are drawn through PCR sequencing identifications
Wei not Bacillus cercus, Alcaligenes faecalis, Arthrobacter protophormiae and Proteus penneri；This 4 plants of bacterium are inoculated with the amount of an every kind of ring
In the test tube containing 5ml liquid agar mediums, mixed bacteria activating solution is prepared into；Configure sucrose 15g/l, yeast extract
25g/l, magnesium sulfate 0.13g/l, sodium chloride 0.11g/l, potassium dihydrogen phosphate 0.11g/l culture medium 1000ml, regulation initial p H
For 5.5, cultivation temperature is 28 DEG C, shaking table culture 12h, and rotating speed is 140r/min, and it is 4% that actication of culture liquid, which connects bacterium amount,（v/v）Carry out
The expansion culture of composite bacteria, the 45g rice husk meals that obtained composite bacteria scale-up medium 400ml is poured into after sterilization treatment
In, supplement after glucose 15g, fermentation 24h, drying and processing 48h under the conditions of 105 DEG C both obtains composite bacteria agent.According to above-mentioned technique system
Standby 3 jin of composite bacteria agents, carry out mixed fermentation 15-30 days, composting material becomes thoroughly decomposed with ready 15 jin of low value catches sample, in
Under the conditions of 105 DEG C dry 48h, grind after cross 20 mesh sieves, packaging both cheap aquatic product compost finished product.The ammonia of compost fermentation finished product
Base acid-state nitrogen content is 0.31%, and content of soluble protein is that the 125.22mg/g contents of organic matter are 49.87%, total nutrient content
For 4.07%, induced worm egg death rate is 98.45%, and coliform group count is 35/g, arsenic, cadmium, lead, chromium, tribute and its compounds content
Reach Standard.
Bacillus cercus of the present invention, Arthrobacter protophormiae, Proteus penneri and Alcaligenes faecalis are public in the literature
Develop table.The document published is respectively：（1）In Isolation and identification [J] of the Bacillus cercuses such as He Yueying
State's feed, 2005, (15):15-16+19；（2）The few cutting edge of a knife or a sword Tilapia mossambica decay processes Bacterial community analysis of Korea Spro and point of spoilage organisms
From, identification and regulation and control [D] the Chinese Academy of Agricultural Sciences, 2010；（3）Chen Yan, waits the plant heights of 1 to imitate the separation identification of poly- phosphorus arthrobacterium
And its poly- phosphorus characteristic research [J] Agriculture of Anhui science, 2011, (01):447-450；（4）Growing nitrogen-fixing is thin in Xing Yong show sugarcanes
Separation, identification and growth characteristics [D] the Guangxi University of bacterium, 2006.4 plants of bacterium of the above can be in Agricultural University Of Shenyang's Foodstuffs Academy
Obtain.
Embodiment 2
Standby after 20 jin of low-value aquatic product catches are rubbed, 5 jin of rice husk meals are placed in autoclave after crossing 20 mesh sieves, set sterilizing bar
121 DEG C of sterilization treatment 20min of part.Two kinds of raw materials are mixed, regulation water content is 58.70%, PH=7, carries out the foundation of compost model,
Fermented and cultured under the conditions of being placed in 15-25 DEG C.In fermentation 1, during 3,5,7,15,21 days, micro- life in separately sampled determination sample
Thing group constitutes, and carries out the extraction of superior microorganism in heap body（Extraction is nutrient agar and casein culture with culture
Base）, then by plate streak separated and purified.Through PCR sequencing identifications draw 4 plants of bacterium be respectively Bacillus cercus,
Alcaligenes faecalis, Arthrobacter protophormiae and Proteus penneri.This 4 plants of bacterium are inoculated in containing 5ml liquid agar with the amount of an every kind of ring
In the test tube of culture medium, mixed bacteria activating solution is prepared into；Configure sucrose 20g/l, yeast extract 30g/l, magnesium sulfate 0.15g/
L, sodium chloride 0.13g/l, potassium dihydrogen phosphate 0.13g/l culture medium 1000ml, regulation initial p H are 6, and cultivation temperature is 30 DEG C,
Shaking table culture 12h, rotating speed is 160r/min, and it is the 5% expansion culture for carrying out composite bacteria to connect bacterium amount, by obtained composite bacteria
Scale-up medium 500ml is poured into the 50g rice husk meals after sterilization treatment, is supplemented after glucose 20g, fermentation 24h, 105 DEG C of conditions
Lower drying and processing 48h, both obtains composite bacteria agent.5 jin of composite bacteria agents are prepared according to above-mentioned technique, with ready 20 jin of low value fishery harvesting
Thing sample carries out mixed fermentation 15-30 days, composting material become thoroughly decomposed under the conditions of 105 DEG C drying 48h, grind after 20 mesh sieves of mistake,
Packaging both obtains cheap aquatic product compost finished product, and the amino acid nitrogen content of compost fermentation finished product is 0.81%, and soluble protein contains
Amount is that the 207.27mg/g contents of organic matter are 53.72%, and total nutrient content is 4.75%, and induced worm egg death rate is 98.82%, large intestine
Flora number is 26/g, and arsenic, cadmium, lead, chromium, tribute and its compounds content reach Standard.For optimal experimental group.
Embodiment 3
Standby after 25 jin of low-value aquatic product catches are rubbed, 7 jin of rice husk meals are placed in autoclave after crossing 20 mesh sieves, set sterilizing bar
121 DEG C of sterilization treatment 20min of part.Two kinds of raw materials are mixed, regulation water content is 56.32%, PH=7.20, carries out building for compost model
It is vertical, fermented and cultured under the conditions of being placed in 15-25 DEG C.It is micro- in separately sampled determination sample in fermentation 1, during 3,5,7,15,21 days
Biocoene is constituted, and the extraction of superior microorganism in heap body is carried out by the method for microorganism plate count（Extraction is with culture
Nutrient agar and casein medium）, again by plate streak separated and purified.Obtained through PCR sequencing identifications
It is respectively Bacillus cercus, Alcaligenes faecalis, Arthrobacter protophormiae and Proteus penneri to go out 4 plants of bacterium.By this 4 plants of bacterium with an every kind of ring
Amount be inoculated in the test tube containing 5ml liquid agar mediums, be prepared into mixed bacteria activating solution, configure sucrose 25g/l, ferment
Mother leaching powder 32g/l, magnesium sulfate 0.17g/l, sodium chloride 0.15g/l, potassium dihydrogen phosphate 0.15g/l culture medium 1000ml, regulation
Initial p H is 6.5, and shaking table culture 12h, rotating speed is 180r/min, and it is the 6% expansion culture for carrying out composite bacteria to connect bacterium amount, will
To composite bacteria scale-up medium take 600ml to pour into the 55g rice husk meals after sterilization treatment, supplement glucose 25g, fermentation
After 24h, 48h is dried under the conditions of 105 DEG C, composite bacteria agent is both obtained.Obtained composite bacteria scale-up medium 600ml is poured into and gone out
In 55g rice husk meals after bacterium processing, supplement after glucose 25g, fermentation 24h, 48h is dried under the conditions of 105 DEG C, compound bacteria is both obtained
Agent.7 jin of composite bacteria agents are prepared according to above-mentioned technique, mixed fermentation 15-30 is carried out with ready 25 jin of low value catches sample
My god, composting material becomes thoroughly decomposed, under the conditions of 105 DEG C drying 48h, grind after cross 20 mesh sieves, pack both cheap aquatic product compost into
Product.The amino acid nitrogen content of compost fermentation finished product is 0.56%, and content of soluble protein is the 168.75mg/g contents of organic matter
For 50.12%, total nutrient content is 4.05%, and induced worm egg death rate is 98.54%, and coliform group count is 54/g, arsenic, cadmium, lead,
Chromium, tribute and its compounds content reach Standard.
Claims (2)
1. a kind of method that utilization cheap aquatic product prepares microorganism compost, it is characterised in that：By cheap aquatic product and rice husk meal
After mixing, regulation heap body carbon-nitrogen ratio is in 30-50%, and water content is in alkalescent in 50-60%, PH, sets up compost, compost fermentation 1,
3rd, 5,7,15, during 21 days, extract superior microorganism carry out it is compound expand culture, for preparing microbial bacterial agent；By microbial inoculum
Mixed with cheap aquatic product raw material, prepare compost, treated its fermentation maturity drying and processing under the conditions of 105 DEG C, that is, obtain compost
Finished product；The notable feature of compost fermentation finished product is solubility Dan white matters Han Liang≤200mg/g.
2. the method that a kind of utilization cheap aquatic product according to claim 1 prepares microorganism compost, it is characterised in that should
Method comprises the following steps：
（1）Feedstock capture：The cheap aquatic product that seashore is adopted back carries out rubbing processing, uses rice husk meal for fermentation substrate, both
It is 4 to add part by weight:1；
（2）Fermentation extracts from compost fermentation body with strain, is respectively Bacillus cercus through strain idenfication, Alcaligenes faecalis, primary
Arthrobacterium and Proteus penneri；
（3）When preparing mixed bacteria liquid activating solution, the inoculum concentration of 4 kinds of bacterium is respectively every kind of 1 ring；The optimum temperature for expanding culture is 30
DEG C, PH is 6, concussion and cultivate 24 hours, and rotating speed is 160r/min；The bacterium amount that connects of mixed bacteria liquid is 5%（v/v）, optimal medium is
Sucrose 20g/l, yeast extract 30g/l, magnesium sulfate 0.15g/l, sodium chloride 0.13g/l, potassium dihydrogen phosphate 0.13g/l；
（4）The preparation of fermenting agent：Composite bacteria scale-up medium 500ml is mixed with 50g rice husk meals, supplement 20g Portugals
Dried and both obtained after grape sugar, fermentation 24h；
（5）Compost fermentation：It regard the composite fermentation microbial inoculum obtained in step (4) as fermentation substrate and the low-value aquatic product for grinding processing
Product carry out mixed fermentation, and fermentation period is 15-30 days；
（6）It is prepared by compost finished product：After fermentation ends, obtained finished product is dried, crushed and packed, low-value aquatic product is both obtained
Product compost finished product.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111689818A (en) * | 2020-06-23 | 2020-09-22 | 慈溪市农业监测中心 | Special formula compound fertilizer for vegetable soybeans and application method thereof |
-
2017
- 2017-06-08 CN CN201710427492.6A patent/CN107056353A/en active Pending
Non-Patent Citations (3)
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| 刘峰等: "鱼类下脚料微生物发酵饲料的初步研究", 《饲料工业》 * |
| 赵玉谨: "发酵鱼类下脚料制备微生物肥料的菌种筛选及工艺研究", 《中国优秀硕士学位论文数据库农业科技编辑》 * |
| 黄琼: "《食品加工技术》", 30 November 2012, 厦门大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111689818A (en) * | 2020-06-23 | 2020-09-22 | 慈溪市农业监测中心 | Special formula compound fertilizer for vegetable soybeans and application method thereof |
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Application publication date: 20170818 |