CN107047943A - The preparation method and applications of cabbage extract and the feed for promoting animal and bird intestines healthy development - Google Patents
The preparation method and applications of cabbage extract and the feed for promoting animal and bird intestines healthy development Download PDFInfo
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- CN107047943A CN107047943A CN201611225479.4A CN201611225479A CN107047943A CN 107047943 A CN107047943 A CN 107047943A CN 201611225479 A CN201611225479 A CN 201611225479A CN 107047943 A CN107047943 A CN 107047943A
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Abstract
The present invention relates to a kind of preparation method and applications of cabbage extract and the feed of promotion animal and bird intestines healthy development.Raw material sources of the present invention are extensive, with low cost, and do not contain any antibiotic, are used as additive by the extract of cabbage, stimulate immunologic function in the enteron aisle of livestock and poultry；Promote intestinal health development, promote growth, improve livestock and poultry daily gain, and reduce the feedstuff-meat ratio of livestock and poultry so that the animal meat quality health cultivated out, meet the safe dietary requirements of consumption, be conducive to widely using in livestock and poultry cultivation.
Description
Technical field
The invention belongs to poultry and livestock feed technical field, and in particular to a kind of preparation method of cabbage extract and its should
With the feed with promotion animal and bird intestines healthy development.
Background technology
Livestock and poultry will guarantee production capacity that will be good, and it is necessary major premise to keep fit, and livestock birds health is primary
Emphasis is that unique access approaches of the various nutriments required for gastrointestinal health, livestock and poultry are intestines and stomach, simultaneously because stomach and intestine
Road is directly communicated with the external world, is also to resist the forefront of extraneous pathogeny infection while being also one of main battle ground, is thus not difficult to find out,
The health and its function of intestines and stomach are normal significant to Production of Livestock and Poultry.
In recent years, many breeding enterprises have abuse and excessively made to promote growth of animal and keep livestock birds health
With the phenomenon of antibiotic and chemicals, as a result cause livestock products medicament residue too high, healthy to consumer is caused
Harmful effect.With the food-safe attention of people, reduce or substitute antibiotics and chemicals will be using will be following livestock and poultry
The inexorable trend of aquaculture development.And plant extracts as feed addictive so that application effect is good, low-residual, be not likely to produce it is anti-
The property of medicine and it is pollution-free and as a kind of preferable feed addictive, be also one of focus of present feed supplement research.But
The plant feed addition of promising result can be reached inexpensively and in terms of animal and bird intestines health is promoted by still lacking a kind of use at present
Agent.
The content of the invention
It is an object of the invention to provide a kind of preparation method and applications of cabbage extract and promotion animal and bird intestines
The feed of healthy development, using the extract of cabbage as additive application in feed, the enteron aisle of livestock and poultry can be promoted to send out
Educate, protect intestinal health, be conducive to improving the feed-weight ratio for delivering weight and reduction livestock and poultry for sale of livestock and poultry.
In order to solve the above technical problems, the technical solution adopted by the present invention is as follows：
A kind of preparation method of cabbage extract is designed, takes fresh cabbage blade plus deionized water to crush and beats
Slurry, and add the material liquid volume ratio of deionized water adjustment slurry to 1:12~18, the ultrasonic-leaching under conditions of 45~55 DEG C
30~60min, gained filtrate is cabbage extract after filtering.
Specifically, taking fresh cabbage blade plus deionized water to crush mashing, and add deionized water adjustment slurry
Material liquid volume ratio is to 1:15, the 70w ultrasonic-leachings 50min under conditions of 50 DEG C, with 250 μm of screen filtrations 2~6 times, most
Gained filtrate is cabbage extract eventually.
Cabbage extract is in application of the animal and fowl fodder as additive obtained by the above method.
Through long-term experimental study, the selected cabbage of present invention screening is the plant of Cruciferae, Brassica genus, is wild cabbage
Mutation, also known as cabbage, cabbage, cabbage, cabbage etc..Wild cabbage has cold-resistant, disease-resistant, strong adaptability, easily stores resistance to fortune, production
The features such as amount height, quality better, generally cultivated all over China.The rich in nutrition content of cabbage, contains per hectogram edible part
1.2~2.4 grams of protein, about 0.3 gram of fat, carbohydrate is about 3.5 grams.And study discovery, knot by inventor
Cabbage is also containing the vitamin enriched, to improving livestock and poultry production performance and promoting animal and bird intestines healthy development to have well
Effect.
Specifically, the feed of the promotion animal and bird intestines healthy development containing the cabbage extract, by following weight
Part raw material is made：
50~57 parts of corn, 19~24 parts of dregs of beans, 5~7 parts of Cottonseed Meal, 2~4 parts of corn protein powder, 2~4 parts of vegetable oil,
7~9 parts of wheat-middlings, 0.3~0.35 part of salt, 0.8~1.2 part of medical stone, 1.2~1.5 parts of stone flour, calcium monohydrogen phosphate 1.5~1.6
Part, 0.8~1.2 part of premix, and the cabbage added according to 50~200mL/kg into above-mentioned raw mixture are extracted
Thing.
Wherein, lysine 14~16%, methionine 3~4%, B B-complex 3%, cupric sulfate pentahydrate 0.7%, a water sulphur
Sour ferrous iron 4.8%, monohydrate zinc sulphate 3.1%, manganese sulfate monohydrate 4.2%, purity is 1% sodium selenite 1.8%, and purity is
1% KI 1.1%, litter shell powder 25%, surplus is medical stone powder.
The present invention compared with prior art, has the following advantages that：
1) raw material sources that feed of the present invention is used are extensive, with low cost, and do not contain any antibiotic, pass through knot
The extract of cabbage is used as additive so that the animal meat quality health that cultivation is delivered for sale, meets the safe dietary requirements of consumption, has
Widely used beneficial in livestock and poultry cultivation.
2) present invention prepares cabbage extract and remains vitamin and other benefit materials to greatest extent, dimension
Raw element U is important bioactive substance, and research shows that vitamin can stimulate immunologic function in animal and bird intestines, and vitamin is to portion
Point harmful intestinal tract bacteria has inhibitory action, can promote animal intestinal tract healthy development, promotes growth, improves livestock and poultry daily gain, and reduce
The feedstuff-meat ratio of livestock and poultry, improves the egg laying performance of birds.
3) in feed of the present invention also contain several amino acids, vitamin and some unknown growth-promoting materials, can reduce ammonia and its
The generation of its corrupt substance, hinders the absorption of harmful substance and waste, reduces the harm to breeding environment.
Brief description of the drawings
Fig. 1 is that the Day-old Broiler Chickens Duodenal villi form Electronic Speculum of control group 49 of broiler trial in embodiment is shone
Piece；
Fig. 2 is that II group of 49 Day-old Broiler Chickens Duodenal villi form Electronic Speculum of experiment of broiler trial in embodiment is shone
Piece；
Fig. 3 is the control group gavage 14d mouse duodenal fluff morphology Electronic Speculum of Experiment on white mice in embodiment
Photo；
Fig. 4 is the experimental group gavage 14d mouse duodenal fluff morphology Electronic Speculum of Experiment on white mice in embodiment
Photo.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Test method or analysis method involved in the present invention, following embodiments is limited, is conventional method unless otherwise instructed,
Agents useful for same is commercially available unless otherwise instructed.
Embodiment 1：A kind of feed of promotion animal and bird intestines healthy development, 56 parts of corn, 22 parts of dregs of beans, 5 parts of Cottonseed Meal, jade
3 parts of rice gluten powder, 3 parts of vegetable oil, 7 parts of wheat-middlings, 0.3 part of salt, 0.8 part of medical stone, 1.3 parts of stone flour, 1.5 parts of calcium monohydrogen phosphate,
1 part of premix, and add cabbage extract 100mL/kg to above-mentioned raw mixture.The constituent mass of the premix
Proportioning is as follows：Lysine 14~16%, methionine 3~4%, B B-complex 3%, cupric sulfate pentahydrate 0.7%, sulfuric acid monohydrate is sub-
Iron 4.8%, monohydrate zinc sulphate 3.1%, manganese sulfate monohydrate 4.2%, purity is 1% sodium selenite 1.8%, and purity is 1%
KI 1.1%, litter shell powder 25%, surplus is medical stone powder.
Embodiment 2：A kind of feed of promotion animal and bird intestines healthy development, 56 parts of corn, 22 parts of dregs of beans, 5 parts of Cottonseed Meal, jade
3 parts of rice gluten powder, 3 parts of vegetable oil, 7 parts of wheat-middlings, 0.3 part of salt, 0.8 part of medical stone, 1.3 parts of stone flour, 1.5 parts of calcium monohydrogen phosphate,
1 part of premix, and add cabbage extract 200mL/kg to above-mentioned raw mixture.The constituent mass of the premix
Proportioning is as follows：Lysine 14~16%, methionine 3~4%, B B-complex 3%, cupric sulfate pentahydrate 0.7%, sulfuric acid monohydrate is sub-
Iron 4.8%, monohydrate zinc sulphate 3.1%, manganese sulfate monohydrate 4.2%, purity is 1% sodium selenite 1.8%, and purity is 1%
KI 1.1%, litter shell powder 25%, surplus is medical stone powder.
Embodiment 3：A kind of feed of promotion animal and bird intestines healthy development, 55 parts of corn, 22 parts of dregs of beans, 6 parts of Cottonseed Meal, jade
3 parts of rice gluten powder, 3 parts of vegetable oil, 7 parts of wheat-middlings, 0.32 part of salt, 0.8 part of medical stone, 1.3 parts of stone flour, calcium monohydrogen phosphate 1.55
Part, 1 part of premix, and add cabbage extract 50mL/kg to above-mentioned raw mixture.The component matter of the premix
Amount proportioning is as follows：Lysine 14~16%, methionine 3~4%, B B-complex 3%, cupric sulfate pentahydrate 0.7%, sulfuric acid monohydrate
Ferrous iron 4.8%, monohydrate zinc sulphate 3.1%, manganese sulfate monohydrate 4.2%, purity is 1% sodium selenite 1.8%, and purity is 1%
KI 1.1%, litter shell powder 25%, surplus is medical stone powder.
The preparation method of cabbage extract described in embodiment 1-3 is as follows：Fresh cabbage blade is taken to add
Ionized water crushes mashing, and adds the material liquid volume ratio of deionized water adjustment slurry to 1:15, the 70w ultrasounds under conditions of 50 DEG C
Ripple extracts 50min, and with 250 μm of screen filtrations four times, final gained filtrate is cabbage extract.
Contrast on effect example：
First, broiler chicken is tested
1.1 experimental designs and management：
Experiment uses single factor test completely randomized design, from the healthy broiler chicken of 3 close ages in days of 150 health, active, body weight
It is randomly divided into 3 treatment groups, respectively control group and experiment 1,2 groups, each 5 repetitions for the treatment of group, 10 broiler chicken of each repetition.
Broiler chicken is prevented epidemic if pre-feeding period 7 days, in pre-feeding period, feeding and drinking-water, then into formal experiment periods.Control group fed is normal
Advise Basic drawing, the feed of the gained of 1 group of feeding embodiment of experiment 3, the feed of the gained of 2 groups of feeding embodiments of experiment 5.
1.2 daily management
Experiment is raised using cage mode in house, and natural lighting, all broiler feeding mixed feeds and are adopted at free water
Food, sterilization and immune is carried out according to a conventional method.Daily evening 20:00 weighs and records every group of feed intake.Two days cleaning up excrement are once.It is real
The state of mind of daily observation broiler chicken, behavior during testing, record every group of daily material consumption, and experimental period is 49 days.
2nd, small white mouse is tested
Healthy female Kunming small white mouse and mouse feed are purchased from the experimental animal center of henan province (certificate of origin used in this experiment
Number NO.4100310000).
2.1 experimental designs and packet
The small female mice in Kunming 24 of health is chosen, 2 groups, every group 12 are randomly divided into.Wherein 1 group is control group, and 2 groups are real
Group is tested, control group gavages physiological saline 0.4ml for every daily, and experimental group small white mouse gavages cabbage extract for every daily
0.6ml.After each group gavage 30 days, 8 fasting 16h are selected at random for every group and butchered, detect index of correlation.
2.2 daily management
Laboratory mouse feeding and management condition is consistent, is raised in units of cage, feeds common mouse daily ration；Small white mouse is daily
After free choice feeding and free water 6 hours, in the morning 10:00 point is prohibited water, fasting, afternoon 15:00 gavage 1 time；Daily with cage
Feed intake is recorded for unit, a mouse weight is recorded weekly；Observe Kunming small white mouse health status and clean in health, cage and pad
Material is changed 2~3 times weekly.
3rd, testing index and method
3.1 meat chicken growth performance
According to weighing and feed intake record, count full period feed consumption rate, calculate full period average daily gain, average daily gain and
Feedstuff-meat ratio.
The meat chicken production performance experimental result of table 1 is contrasted
3.2 Intestine of Broiler development indexs are determined
In 49 age in days of broiler chicken, every group is selected 10 chickens to butcher at random, and (stomach and bile duct are in small intestine for clip duodenum in experiment
Opening between, at pylorus 175cm), the portion such as jejunum (jejunum stage casing) and ileum (part between ileocaecal sphineter film and colon)
Position each 3cm of intestinal tube, content is removed with normal saline flushing, fixed preservation is put into 10% formalin, for intestinal tissue
The measure of structure index.
Go bail for keep it is broken-hearted, be dehydrated step by step with ethanol, dimethylbenzene is transparent, embedding machine embedding, slicer cut into slices
((4um), is dyed with h and E, gummy mounting, and photomicrograph equipment carries out IMAQ (see accompanying drawing 1-2), slide
Under 40X and 100X multiplication factors histotomy is carried out with light microscopy and then with the softwares of Motic Images 2000
The measurement of height of naps and Crypt depth.Suede gland ratio=height of naps (um)/Crypt depth (um).Index determining is herded in Henan
Industry institute of economics Physiological Experiment room is carried out.
Influence (um) of the cabbage extract of table 2 to broiler chicken 49d Morphological Indices of Duodenum Villus
3.3 small white mouse intestinal growth index determinings
During 30 age in days of small white mouse, every group is selected 8 at random, clip its duodenum (stomach and bile duct at the opening of small intestine it
Between) each 1cm of intestinal tube, content is removed with normal saline flushing, fixed preservation is put into 10% formalin, for intestinal tissue
The measure of structure index.
Go bail for keep it is broken-hearted, be dehydrated step by step with ethanol, dimethylbenzene is transparent, embedding machine embedding, slicer cut into slices
((4um), is dyed with h and E, gummy mounting, and photomicrograph equipment carries out IMAQ (see accompanying drawing 3-4), suede gland ratio
Example=height of naps (um)/Crypt depth (um).Index determining is carried out in Henan animal husbandry institute of economics Physiological Experiment room.
Influence (um) of the cabbage extract of table 3 to mouse 21d Morphological Indices of Duodenum Villus
3.4 data processing
Variance analysis and Multiple range test (LSD methods) are carried out to data using SPSS statistical softwares 18.0.
4th, interpretation of result
The change of intestinal tissue form and the production performance of livestock and poultry have much relations, and the structure of intestinal mucosa can be shown
The relevant information of function of intestinal canal, such as height of naps, Crypt depth and enteron aisle epidermis area.Increase villus length, which can increase, to disappear
Change absorption area, strengthen the effect of digestive ferment and the transport of nutriment, and then strengthen the digestion and absorption function of enteron aisle.The present invention
Research shows, cabbage extract is added in broiler chickens mixed feed, Duodenal villi can be obviously promoted and small intestine is smooth
Flesh is developed, wherein, when cabbage extract addition is 200mL/kg, 49 days Duodenal villi height and small intestine are smooth
Flesh thickness and control group significant difference (P<0.05).Cabbage extract can improve meat chicken production performance, reduce feedstuff-meat ratio, when
When cabbage extract addition is 200mL/kg, weight and control group significant difference (P are delivered within 49 days for sale<0.05)；Kunming little Bai
Mouse experiment shows, cabbage extract is added in small white mouse daily ration, Duodenal villi length and suede gland can be significantly improved
Than, by increasing capacitance it is possible to increase colonic smooth muscle thickness.Test result indicates that, cabbage extract can promote animal and bird intestines to develop and tie up
Protect intestinal health.
Claims (5)
1. a kind of preparation method of cabbage extract, it is characterised in that take fresh cabbage blade plus deionized water
Mashing is crushed, and adds the material liquid volume ratio of deionized water adjustment slurry to 1:12~18, it is ultrasonic under conditions of 45~55 DEG C
Ripple extracts 30~60min, and gained filtrate is cabbage extract after filtering.
2. the preparation method of cabbage extract as claimed in claim 1, it is characterised in that
Take fresh cabbage blade plus deionized water to crush mashing, and add the material liquid volume ratio of deionized water adjustment slurry extremely
1：15, with 70w ultrasonic-leaching 50min under conditions of 50 DEG C, with 250 μm of screen filtrations 2~6 times, final gained filtrate
As cabbage extract.
3. if claim 1 gained cabbage extract is in application of the animal and fowl fodder as additive.
4. a kind of feed of promotion animal and bird intestines healthy development, it is characterised in that its preparing raw material includes：
The mixture being made up of the raw material of following parts by weight：50~57 parts of corn, 19~24 parts of dregs of beans, 5~7 parts of Cottonseed Meal, jade
2~4 parts of rice gluten powder, 2~4 parts of vegetable oil, 7~9 parts of wheat-middlings, 0.3~0.35 part of salt, 0.8~1.2 part of medical stone, stone flour
1.2~1.5 parts, 1.5~1.6 parts of calcium monohydrogen phosphate, 0.8~1.2 part of premix；
And the gained cabbage extract of claim 1 or 2, its addition is according to 50~200mL/kg above-mentioned raw materials mixtures
Calculate.
5. promote the feed of animal and bird intestines healthy development as claimed in claim 4, it is characterised in that by weight percentage,
The premix raw material composition is as follows：Lysine 14~16%, methionine 3~4%, B B-complex 3%, cupric sulfate pentahydrate 0.7%,
Ferrous sulfate monohydrate 4.8%, monohydrate zinc sulphate 3.1%, manganese sulfate monohydrate 4.2%, purity is 1% sodium selenite 1.8%, and purity is
1% KI 1.1%, litter shell powder 25%, surplus is medical stone powder.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114601068A (en) * | 2022-02-28 | 2022-06-10 | 河南牧业经济学院 | Growth-promoting feed additive for improving intestinal flora of broiler chicken and preparation and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102940160A (en) * | 2012-12-10 | 2013-02-27 | 四川铁骑力士实业有限公司 | Poult compound feed for promoting development of digestive tracts of chickens and preparation method thereof |
| CN103494001A (en) * | 2013-08-27 | 2014-01-08 | 安徽省瑞森生物科技有限责任公司 | Laying hen forage and preparation method thereof |
| CN105104903A (en) * | 2015-09-29 | 2015-12-02 | 河南牧业经济学院 | Broiler compound feed with purple cabbage extracts and method for manufacturing broiler compound feed |
-
2016
- 2016-12-27 CN CN201611225479.4A patent/CN107047943A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102940160A (en) * | 2012-12-10 | 2013-02-27 | 四川铁骑力士实业有限公司 | Poult compound feed for promoting development of digestive tracts of chickens and preparation method thereof |
| CN103494001A (en) * | 2013-08-27 | 2014-01-08 | 安徽省瑞森生物科技有限责任公司 | Laying hen forage and preparation method thereof |
| CN105104903A (en) * | 2015-09-29 | 2015-12-02 | 河南牧业经济学院 | Broiler compound feed with purple cabbage extracts and method for manufacturing broiler compound feed |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114601068A (en) * | 2022-02-28 | 2022-06-10 | 河南牧业经济学院 | Growth-promoting feed additive for improving intestinal flora of broiler chicken and preparation and application thereof |
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Application publication date: 20170818 |