CN107046848A - A kind of processing method of watermelon seed - Google Patents
A kind of processing method of watermelon seed Download PDFInfo
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- CN107046848A CN107046848A CN201710159139.4A CN201710159139A CN107046848A CN 107046848 A CN107046848 A CN 107046848A CN 201710159139 A CN201710159139 A CN 201710159139A CN 107046848 A CN107046848 A CN 107046848A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
A kind of processing method of watermelon seed of disclosure of the invention, including, seed preparation, prepare treatment fluid, treatment fluid A processing, treatment fluid B processing, carbon dioxide treatment, treatment fluid C processing.The present invention is handled watermelon seed respectively successively by the various concentrations treatment fluid of preparation, the increasing of watermelon seedlings, the accumulation of watermelon dry can be promoted to a certain extent, and promote its root growth and improve root activity, increase chlorophyll content, increasing for mda content can also be suppressed simultaneously, so as to promote the growth of watermelon seedlings.
Description
Technical field
The present invention relates to field of planting, more particularly to a kind of processing method of watermelon seed.
Background technology
Watermelon is Curcurbitaceae Citrullus, and watermelon originates in South Africa, in the southern group that there are various watermelon wild species, have a sweet tea and
It is edible, also have sweetless but capable of using as feed, have bitter without edible, watermelon juice multi-flavor sweet tea, the cultivated area of China is very wide
General, watermelon area accounts for more than the 60% of world's gross area, and yield accounts for 70% or so, with the development of society, watermelon has no longer been season
The section property consumer goods, and it is increasingly becoming nutraceutical.
Watermelon seed typically can all be handled to improve germination percentage, and existing processing method also has many kinds, hangs
Well method, refrigeration, mix micromicrofarad etc..Different processing modes, the different effect of correspondence, these conventional processing methods can
Percentage of seedgermination is improved to a certain extent, shortens germination time, mitigates Field diseases, and seedling seedling is neat, Miao Zhuan.But, it is existing
Some processing methods improve limited to Germination of Watermelon Seed rate, and on watermelon seedlings root activity, the influence of photosynthesis characteristics aspect not
Greatly.
The content of the invention
It is an object of the invention to provide a kind of processing method of watermelon seed, to improve the germination percentage of watermelon seed.
The present invention is achieved through the following technical solutions：
A kind of processing method of watermelon seed, comprises the following steps：
A seed preparations：Ripe watermelon seed is gathered, full grains are selected, the seed of no disease and pests harm first uses these seeds
25-28 DEG C of clear water immersion 15-18min, then pulls out, drains naturally；
B prepares treatment fluid：Handling liquid and preparation method thereof is：Duck wheat, Radix Baccaureae ramiflorae are cleaned, the removal of impurity is gone, is crushed to 100 mesh, plus
The water of duck wheat, Radix Baccaureae ramiflorae mixing quality 70%, is heated after boiling 20min, natural cooling, is inoculated with inoculated mixture quality
0.03% aspergillus oryzae, loads blind boxes, is placed at 32 DEG C and is cultivated, and incubation time is 20 hours, then by gained
Culture adds water, in water: weight of material ratio be 1: 2,10min is incubated at 45 DEG C, then with 2000r/min rotating speed centrifugations at
20min is managed, 2 hours are stood, then the ultrasonication 2min for then using power to be 40KHz for 450W, frequency carries out solid-liquid
Separation, retains filtrate, after filtrate is sterilized, and adds the glucose of filtrate quality 2%, after stirring, and is inoculated with monascus ruber
Kind, inoculum concentration is filtrate quality 0.02%, after being cultivated 25 hours at 32 DEG C, then carries out sterilization treatment, obtains treatment fluid；By hardship
Buckwheat and Radix Baccaureae ramiflorae different quality ratio are prepared into following three kinds for the treatment of fluids：Treatment fluid A：Duck wheat is 1 with Radix Baccaureae ramiflorae mass ratio：
1, treatment fluid B：Duck wheat is 3 with Radix Baccaureae ramiflorae mass ratio：1, treatment fluid C：Duck wheat is 6 with Radix Baccaureae ramiflorae mass ratio：1；
C treatment fluid A processing：The watermelon seed that will be handled in step a, 1 is pressed with treatment fluid A：After the mixing of 10 mass ratios, by temperature
Regulation is stirred 2 hours with 30r/min rotating speeds, then taken out to 5-8 DEG C, is cleaned surface using clear water, is drained naturally；
D treatment fluid B processing：The watermelon seed that will be handled in step c, 1 is pressed with treatment fluid B：After the mixing of 10 mass ratios, by temperature
Regulation is stirred 2 hours with 50r/min rotating speeds, then taken out to 18-20 DEG C, is cleaned surface using clear water, is drained naturally；
E carbon dioxide treatments：The step d watermelon seeds handled are put into closed no light conditions, gas concentration lwevel in environment
Keep fixing, be stably maintained at 455mg/kg, after 1 hour, take out；
F treatment fluid C processing：The watermelon seed that step e is handled, 1 is pressed with treatment fluid C：After the mixing of 10 mass ratios, temperature is adjusted
Section is stirred 2 hours with 80r/min rotating speeds, then taken out to 28-30 DEG C, is cleaned surface using clear water, is drained naturally.
The beneficial effects of the invention are as follows：The present invention is divided watermelon seed successively by the various concentrations treatment fluid of preparation
Other places are managed, and can promote the increasing of watermelon seedlings, the accumulation of watermelon dry to a certain extent, and promote its root growth and
Root activity, increase chlorophyll content are improved, while can also suppress increasing for mda content, so as to promote the life of watermelon seedlings
It is long, by being handled after treatment fluid B handles watermelon seed, then using the carbon dioxide of fixed concentration watermelon seed, energy
Enough so that seed endoperoxides hydrogenase activity, Acid Phosphatase Activity and the amount of ATP of germination period are significantly improved,
And the vigor of the height of catalase activity and seed causes watermelon into positive correlation by improving the activity of catalase
Physiological metabolism is more vigorous in seed, and Acid Phosphatase Activity gradually increases with the sprouting process of seed, its active height
The low speed for showing seed sprouting, by improving Acid Phosphatase Activity, to accelerate the sprouting of watermelon seed, finally by
Consolidations of the treatment fluid C to watermelon seed is handled, and can further promote watermelon seed vigor, improves Germination of Watermelon Seed rate, and
And there is significant facilitation to the growth of watermelon seedlings lateral root.
Embodiment
Embodiment 1
A kind of processing method of watermelon seed, comprises the following steps：
A seed preparations：Ripe watermelon seed is gathered, full grains, the seed of no disease and pests harm, by these seeds first with 25 are selected
DEG C clear water immersion 15min, then pull out, drain naturally；
B prepares treatment fluid：Handling liquid and preparation method thereof is：Duck wheat, Radix Baccaureae ramiflorae are cleaned, the removal of impurity is gone, is crushed to 100 mesh, plus
The water of duck wheat, Radix Baccaureae ramiflorae mixing quality 70%, is heated after boiling 20min, natural cooling, is inoculated with inoculated mixture quality
0.03% aspergillus oryzae, loads blind boxes, is placed at 32 DEG C and is cultivated, and incubation time is 20 hours, then by gained
Culture adds water, in water: weight of material ratio be 1: 2,10min is incubated at 45 DEG C, then with 2000r/min rotating speed centrifugations at
20min is managed, 2 hours are stood, then the ultrasonication 2min for then using power to be 40KHz for 450W, frequency carries out solid-liquid
Separation, retains filtrate, after filtrate is sterilized, and adds the glucose of filtrate quality 2%, after stirring, and is inoculated with monascus ruber
Kind, inoculum concentration is filtrate quality 0.02%, after being cultivated 25 hours at 32 DEG C, then carries out sterilization treatment, obtains treatment fluid；By hardship
Buckwheat and Radix Baccaureae ramiflorae different quality ratio are prepared into following three kinds for the treatment of fluids：Treatment fluid A：Duck wheat is 1 with Radix Baccaureae ramiflorae mass ratio：
1, treatment fluid B：Duck wheat is 3 with Radix Baccaureae ramiflorae mass ratio：1, treatment fluid C：Duck wheat is 6 with Radix Baccaureae ramiflorae mass ratio：1；
C treatment fluid A processing：The watermelon seed that will be handled in step a, 1 is pressed with treatment fluid A：After the mixing of 10 mass ratios, by temperature
Regulation is stirred 2 hours with 30r/min rotating speeds, then taken out to 5 DEG C, is cleaned surface using clear water, is drained naturally；
D treatment fluid B processing：The watermelon seed that will be handled in step c, 1 is pressed with treatment fluid B：After the mixing of 10 mass ratios, by temperature
Regulation is stirred 2 hours with 50r/min rotating speeds, then taken out to 18 DEG C, is cleaned surface using clear water, is drained naturally；
E carbon dioxide treatments：The step d watermelon seeds handled are put into closed no light conditions, gas concentration lwevel in environment
Keep fixing, be stably maintained at 455mg/kg, after 1 hour, take out；
F treatment fluid C processing：The watermelon seed that step e is handled, 1 is pressed with treatment fluid C：After the mixing of 10 mass ratios, temperature is adjusted
Section is stirred 2 hours with 80r/min rotating speeds, then taken out to 28 DEG C, is cleaned surface using clear water, is drained naturally.
Embodiment 2
A kind of processing method of watermelon seed, comprises the following steps：
A seed preparations：Ripe watermelon seed is gathered, full grains, the seed of no disease and pests harm, by these seeds first with 28 are selected
DEG C clear water immersion 18min, then pull out, drain naturally；
B prepares treatment fluid：Handling liquid and preparation method thereof is：Duck wheat, Radix Baccaureae ramiflorae are cleaned, the removal of impurity is gone, is crushed to 100 mesh, plus
The water of duck wheat, Radix Baccaureae ramiflorae mixing quality 70%, is heated after boiling 20min, natural cooling, is inoculated with inoculated mixture quality
0.03% aspergillus oryzae, loads blind boxes, is placed at 32 DEG C and is cultivated, and incubation time is 20 hours, then by gained
Culture adds water, in water: weight of material ratio be 1: 2,10min is incubated at 45 DEG C, then with 2000r/min rotating speed centrifugations at
20min is managed, 2 hours are stood, then the ultrasonication 2min for then using power to be 40KHz for 450W, frequency carries out solid-liquid
Separation, retains filtrate, after filtrate is sterilized, and adds the glucose of filtrate quality 2%, after stirring, and is inoculated with monascus ruber
Kind, inoculum concentration is filtrate quality 0.02%, after being cultivated 25 hours at 32 DEG C, then carries out sterilization treatment, obtains treatment fluid；By hardship
Buckwheat and Radix Baccaureae ramiflorae different quality ratio are prepared into following three kinds for the treatment of fluids：Treatment fluid A：Duck wheat is 1 with Radix Baccaureae ramiflorae mass ratio：
1, treatment fluid B：Duck wheat is 3 with Radix Baccaureae ramiflorae mass ratio：1, treatment fluid C：Duck wheat is 6 with Radix Baccaureae ramiflorae mass ratio：1；
C treatment fluid A processing：The watermelon seed that will be handled in step a, 1 is pressed with treatment fluid A：After the mixing of 10 mass ratios, by temperature
Regulation is stirred 2 hours with 30r/min rotating speeds, then taken out to 8 DEG C, is cleaned surface using clear water, is drained naturally；
D treatment fluid B processing：The watermelon seed that will be handled in step c, 1 is pressed with treatment fluid B：After the mixing of 10 mass ratios, by temperature
Regulation is stirred 2 hours with 50r/min rotating speeds, then taken out to 20 DEG C, is cleaned surface using clear water, is drained naturally；
E carbon dioxide treatments：The step d watermelon seeds handled are put into closed no light conditions, gas concentration lwevel in environment
Keep fixing, be stably maintained at 455mg/kg, after 1 hour, take out；
F treatment fluid C processing：The watermelon seed that step e is handled, 1 is pressed with treatment fluid C：After the mixing of 10 mass ratios, temperature is adjusted
Section is stirred 2 hours with 80r/min rotating speeds, then taken out to 30 DEG C, is cleaned surface using clear water, is drained naturally.
Embodiment 3
A kind of processing method of watermelon seed, comprises the following steps：
A seed preparations：Ripe watermelon seed is gathered, full grains, the seed of no disease and pests harm, by these seeds first with 27 are selected
DEG C clear water immersion 16min, then pull out, drain naturally；
B prepares treatment fluid：Handling liquid and preparation method thereof is：Duck wheat, Radix Baccaureae ramiflorae are cleaned, the removal of impurity is gone, is crushed to 100 mesh, plus
The water of duck wheat, Radix Baccaureae ramiflorae mixing quality 70%, is heated after boiling 20min, natural cooling, is inoculated with inoculated mixture quality
0.03% aspergillus oryzae, loads blind boxes, is placed at 32 DEG C and is cultivated, and incubation time is 20 hours, then by gained
Culture adds water, in water: weight of material ratio be 1: 2,10min is incubated at 45 DEG C, then with 2000r/min rotating speed centrifugations at
20min is managed, 2 hours are stood, then the ultrasonication 2min for then using power to be 40KHz for 450W, frequency carries out solid-liquid
Separation, retains filtrate, after filtrate is sterilized, and adds the glucose of filtrate quality 2%, after stirring, and is inoculated with monascus ruber
Kind, inoculum concentration is filtrate quality 0.02%, after being cultivated 25 hours at 32 DEG C, then carries out sterilization treatment, obtains treatment fluid；By hardship
Buckwheat and Radix Baccaureae ramiflorae different quality ratio are prepared into following three kinds for the treatment of fluids：Treatment fluid A：Duck wheat is 1 with Radix Baccaureae ramiflorae mass ratio：
1, treatment fluid B：Duck wheat is 3 with Radix Baccaureae ramiflorae mass ratio：1, treatment fluid C：Duck wheat is 6 with Radix Baccaureae ramiflorae mass ratio：1；
C treatment fluid A processing：The watermelon seed that will be handled in step a, 1 is pressed with treatment fluid A：After the mixing of 10 mass ratios, by temperature
Regulation is stirred 2 hours with 30r/min rotating speeds, then taken out to 7 DEG C, is cleaned surface using clear water, is drained naturally；
D treatment fluid B processing：The watermelon seed that will be handled in step c, 1 is pressed with treatment fluid B：After the mixing of 10 mass ratios, by temperature
Regulation is stirred 2 hours with 50r/min rotating speeds, then taken out to 19 DEG C, is cleaned surface using clear water, is drained naturally；
E carbon dioxide treatments：The step d watermelon seeds handled are put into closed no light conditions, gas concentration lwevel in environment
Keep fixing, be stably maintained at 455mg/kg, after 1 hour, take out；
F treatment fluid C processing：The watermelon seed that step e is handled, 1 is pressed with treatment fluid C：After the mixing of 10 mass ratios, temperature is adjusted
Section is stirred 2 hours with 80r/min rotating speeds, then taken out to 29 DEG C, is cleaned surface using clear water, is drained naturally.
Comparative example 1：Step step cdf is differed only in embodiment 1 and uses treatment fluid A processing, and processing method is constant.
Comparative example 2：Step step cdf is differed only in embodiment 1 and uses treatment fluid B processing, and processing method is constant.
Comparative example 3：Step step cdf is differed only in embodiment 1 and uses treatment fluid C processing, and processing method is constant.
Experiment：The glad watermelon seed in capital is selected, respectively to embodiment（Average）With the seed that is handled in comparative example in phase
After being showed money or valuables one carries unintentionally with vernalization under environment to 70% seed, sowed, its cultivation matrix selects high-quality crop field topsoil, using 13cm
The plastic tub of × 15cm specifications is potted plant, and 3 seeds are sowed per basin, after emerging, and retains 1 plant, in the 4th leaf of seedling to its leaf
Chlorophyll contents, root activity and MDA are measured：
Measuring chlorophyll content is measured using AAS, root activity is determined and determined using TTC methods（Choose watermelon root
Sharp sample 0.2g is put into 50mL beakers, adds the equivalent mixed liquor 10mL of 0.4%TTC solution and phosphate buffer, and root is abundant
In the solution, dark insulation 3h, then adds 1mol/L sulfuric acid 2mL at 37 DEG C, to stop reaction, while making a blank for submergence
Experiment, suck dry moisture after the tip of a root takes out, adds ethyl acetate 3mL and a small amount of quartz sand is ground together, then filters and uses acetic acid
Ethyl ester is settled to 10mL, using spectrophotometer under 485nm colorimetric, calculate TTC reduction intensities mg/gh）, mda content
Determine and use thiobarbituricacidα- development process；
Table 1
As can be seen from Table 1, the processing energy for the treatment of fluid A, treatment fluid B and treatment fluid C to watermelon seed in processing method of the present invention
The chlorophyll, root activity, mda content of watermelon seedlings are enough significantly affected, single treatment fluid treatment effect is substantially reduced.
Claims (1)
1. a kind of processing method of watermelon seed, it is characterised in that comprise the following steps：
A seed preparations：Ripe watermelon seed is gathered, full grains are selected, the seed of no disease and pests harm first uses these seeds
25-28 DEG C of clear water immersion 15-18min, then pulls out, drains naturally；
B prepares treatment fluid：Handling liquid and preparation method thereof is：Duck wheat, Radix Baccaureae ramiflorae are cleaned, the removal of impurity is gone, is crushed to 100 mesh, plus
The water of duck wheat, Radix Baccaureae ramiflorae mixing quality 70%, is heated after boiling 20min, natural cooling, is inoculated with inoculated mixture quality
0.03% aspergillus oryzae, loads blind boxes, is placed at 32 DEG C and is cultivated, and incubation time is 20 hours, then by gained
Culture adds water, in water: weight of material ratio be 1: 2,10min is incubated at 45 DEG C, then with 2000r/min rotating speed centrifugations at
20min is managed, 2 hours are stood, then the ultrasonication 2min for then using power to be 40KHz for 450W, frequency carries out solid-liquid
Separation, retains filtrate, after filtrate is sterilized, and adds the glucose of filtrate quality 2%, after stirring, and is inoculated with monascus ruber
Kind, inoculum concentration is filtrate quality 0.02%, after being cultivated 25 hours at 32 DEG C, then carries out sterilization treatment, obtains treatment fluid；By hardship
Buckwheat and Radix Baccaureae ramiflorae different quality ratio are prepared into following three kinds for the treatment of fluids：Treatment fluid A：Duck wheat is 1 with Radix Baccaureae ramiflorae mass ratio：
1, treatment fluid B：Duck wheat is 3 with Radix Baccaureae ramiflorae mass ratio：1, treatment fluid C：Duck wheat is 6 with Radix Baccaureae ramiflorae mass ratio：1；
C treatment fluid A processing：The watermelon seed that will be handled in step a, 1 is pressed with treatment fluid A：After the mixing of 10 mass ratios, by temperature
Regulation is stirred 2 hours with 30r/min rotating speeds, then taken out to 5-8 DEG C, is cleaned surface using clear water, is drained naturally；
D treatment fluid B processing：The watermelon seed that will be handled in step c, 1 is pressed with treatment fluid B：After the mixing of 10 mass ratios, by temperature
Regulation is stirred 2 hours with 50r/min rotating speeds, then taken out to 18-20 DEG C, is cleaned surface using clear water, is drained naturally；
E carbon dioxide treatments：The step d watermelon seeds handled are put into closed no light conditions, gas concentration lwevel in environment
Keep fixing, be stably maintained at 455mg/kg, after 1 hour, take out；
F treatment fluid C processing：The watermelon seed that step e is handled, 1 is pressed with treatment fluid C：After the mixing of 10 mass ratios, temperature is adjusted
Section is stirred 2 hours with 80r/min rotating speeds, then taken out to 28-30 DEG C, is cleaned surface using clear water, is drained naturally.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710159139.4A CN107046848A (en) | 2017-03-17 | 2017-03-17 | A kind of processing method of watermelon seed |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710159139.4A CN107046848A (en) | 2017-03-17 | 2017-03-17 | A kind of processing method of watermelon seed |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107969295A (en) * | 2017-12-08 | 2018-05-01 | 颍上县心农甜西甜瓜种植专业合作社 | A kind of western Muskmelon Planting method of preventing disease and pest |
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| CN104838766A (en) * | 2015-06-10 | 2015-08-19 | 安徽徽大农业有限公司 | Germination method for water melon seeds |
| CN104885618A (en) * | 2014-09-29 | 2015-09-09 | 安徽盛创农业科技有限公司 | Watermelon seed germination method |
| CN104885625A (en) * | 2014-10-06 | 2015-09-09 | 安徽国盛农业科技有限责任公司 | Germinating method for watermelon seeds |
| CN105993287A (en) * | 2016-07-13 | 2016-10-12 | 安徽菲扬农业科技有限公司 | Watermelon seed germination method |
| CN106134571A (en) * | 2016-07-13 | 2016-11-23 | 合肥隆扬农业科技有限公司 | A kind of germination method of watermelon seed |
| CN106416499A (en) * | 2016-08-25 | 2017-02-22 | 合肥市田然农业科技园有限公司 | Method for accelerating germination of watermelon seeds |
-
2017
- 2017-03-17 CN CN201710159139.4A patent/CN107046848A/en not_active Withdrawn
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104885618A (en) * | 2014-09-29 | 2015-09-09 | 安徽盛创农业科技有限公司 | Watermelon seed germination method |
| CN104885625A (en) * | 2014-10-06 | 2015-09-09 | 安徽国盛农业科技有限责任公司 | Germinating method for watermelon seeds |
| CN104838766A (en) * | 2015-06-10 | 2015-08-19 | 安徽徽大农业有限公司 | Germination method for water melon seeds |
| CN105993287A (en) * | 2016-07-13 | 2016-10-12 | 安徽菲扬农业科技有限公司 | Watermelon seed germination method |
| CN106134571A (en) * | 2016-07-13 | 2016-11-23 | 合肥隆扬农业科技有限公司 | A kind of germination method of watermelon seed |
| CN106416499A (en) * | 2016-08-25 | 2017-02-22 | 合肥市田然农业科技园有限公司 | Method for accelerating germination of watermelon seeds |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107969295A (en) * | 2017-12-08 | 2018-05-01 | 颍上县心农甜西甜瓜种植专业合作社 | A kind of western Muskmelon Planting method of preventing disease and pest |
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Application publication date: 20170818 |