CN107044886A - A kind of multiband spectrum imaging source, imaging method and microscope - Google Patents
A kind of multiband spectrum imaging source, imaging method and microscope Download PDFInfo
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- 238000003384 imaging method Methods 0.000 title claims abstract description 46
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- 238000005516 engineering process Methods 0.000 description 7
- 238000004020 luminiscence type Methods 0.000 description 5
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/2823—Imaging spectrometer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
- G01J3/0208—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using focussing or collimating elements, e.g. lenses or mirrors; performing aberration correction
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B17/00—Systems with reflecting surfaces, with or without refracting elements
- G02B17/08—Catadioptric systems
- G02B17/0856—Catadioptric systems comprising a refractive element with a reflective surface, the reflection taking place inside the element, e.g. Mangin mirrors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
- G01J2003/102—Plural sources
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/2823—Imaging spectrometer
- G01J2003/2826—Multispectral imaging, e.g. filter imaging
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Abstract
The invention provides a kind of multiband spectrum imaging source, imaging method and microscope, multiband spectrum imaging source therein, including：First combined light source, the first collimating mirror, speculum, the first semi-transparent semi-reflecting eyeglass, the second combined light source, the second collimating mirror.First combined light source, can at least send the light of more than one monochromatic wavelength, and after the first collimating mirror collimation, with the minute surface of the first incident angle directive speculum；First semi-transparent semi-reflecting eyeglass, is located in the first light path between first combined light source and the speculum；Second combined light source, the second combined light source is located at the position beyond first light path.A kind of multiband spectrum imaging source and imaging method that the present invention is provided, with low cost, the effect of fast imaging, using this method and light source, the multiband multispectral imaging of the 60 frames/more than second can be realized, the requirement of most of medical science multispectral imaging application is met.
Description
Technical field
The present invention relates to medical science spectral imaging technology field, specifically a kind of multiband spectrum imaging source, imaging
Method and microscope, quantitative point for the positioning and quantitative analysis of cell component, the particularly intracellular members of medical domain
Analysis.
Background technology
Spectral imaging technology originates from the multispectral romote sensing technology of 1970s, and the need applied with remote sensing of the earth
The most important characteristics and mark wanted and develop spectral imaging technologies are that spectrum and image are combined as a whole, the earth acquired in it
Surface image contains abundant space, radiation and the triple information of spectrum, thus in geology, agricultural, vegetation, environment, city, army
There is good application in terms of thing, the hydrology, air.
Spectral imaging technology is applied to the detection of biological organization sample, it is qualitative, fixed that biological tissue's spectroscopic data is carried out
Amount and positioning analysis, can really realize to biological tissue carry out " where place, have what, how many " comprehensive analysis target,
So as to realize the early diagnosis to some pathological changes.
The method that light spectrum image-forming is realized mainly has three kinds at present：
1. using the automatically controlled modulation device filters of the AOTF based on electroacoustic light principle or the principle of interference based on polarised light LCTF
Light, the image after filtering is gathered with CCD.The short slab of this kind of method is the inhomogeneities of automatically controlled light filter part, veiling glare and low
Transmitance influence is larger, and cost of implementation is higher.
2. the automatically controlled filter wheel being made of thin-film interference filterses is filtered, different optical filters are turned to, are gathered with CCD
Image after different wave length optical filtering.Have the disadvantage to be restricted by filter wheel switch speed, image taking speed is slower.
3. the principle of pull-broom type imaging spectrometer in air remote sensing is continued to use, using prism or grating combination element in rear optics
System carries out spectrum, and push-scanning image is carried out using the driving of high-precision objective table automatics.Advantage be spectral resolution compared with
Height, has the disadvantage that image taking speed is still very slow.
The content of the invention
The invention provides a kind of multiband spectrum imaging source, imaging method and microscope, medical domain is solved quick
The problem of multispectral imaging.
To reach above-mentioned purpose, the present invention is implemented by the following technical programs：
Following title meaning described in this specification is：
Discrete monochromatic light, refers to the monochromatic light combination of multiple monochromatic wavelength of more than one (containing one).
Combined light source, the discrete monochromatic light source of more than one (containing one) multiple monochromatic wavelength combination can be sent by referring to.
A kind of multiband spectrum imaging source, including：
First combined light source, can at least send the discrete monochromatic light of first wave length, and after the first collimating mirror collimation
With the minute surface of the first incident angle directive speculum；
Speculum, for receiving the incident light of first combined light source and reflecting away, first combined light source with
The first light path is formed between the speculum；
First half-reflection and half-transmission eyeglass, is located in the first light path between first monochromatic source and the speculum, institute
Stating the first half-reflection and half-transmission eyeglass includes mutually deviating from and parallel the first minute surface and the second minute surface, and first combined light source is with the
First minute surface of the first half-reflection and half-transmission eyeglass described in one incident angle directive；
Second combined light source, can send the discrete monochromatic light of second wave length, and the second combined light source is located at first light path
Position in addition, and second combined light source is with the second mirror of the first semi-transparent semi-reflecting eyeglass described in the second incident angle directive
Face；
Wherein, the first wave length and the second wave length are unequal, and first incident angle 30-60 degree it
Between, second incident angle is between 30-60 degree.
Preferably, the first collimation lens, first combined light source are provided with the exit direction of first combined light source
On the photocentre of first collimation lens；
And/or, the exit direction of second combined light source is provided with the second collimation lens, the second combined light source position
In on the photocentre of second collimation lens.
Preferably, first combined light source and/or second combined light source can send one or more kinds of ripples
Long discrete monochromatic light, and the discrete monochromatic wavelength that the first combined light source, the second combined light source can be sent is unequal.
Preferably, the described first semi-transparent semi-reflecting eyeglass is dichroic color filter piece, or the first semi-transparent semi-reflecting eyeglass
The metal film glass reflected for fractional transmission part.
Preferably, in addition to：
Second semi-transparent semi-reflecting eyeglass, is located in the light path between the described first semi-transparent semi-reflecting eyeglass and the speculum, institute
Stating the second semi-transparent semi-reflecting eyeglass includes mutually deviating from and the 3rd parallel minute surface and the 4th minute surface, and first combined light source is with the
3rd minute surface of the second semi-transparent semi-reflecting eyeglass described in three incident angle directives；
3rd combined light source, can at least send the discrete monochromatic light of the 3rd wavelength, the 3rd combined light source is with the 3rd
4th minute surface of the second semi-transparent semi-reflecting eyeglass described in incident angle directive；
Wherein, the 3rd wavelength and the first wave length, second wave length are unequal, and the 3rd incident angle is in 30-60 degree
Between.
Preferably, there is first wave length between the first wave length, second wave length, the 3rd wavelength<Second wave length<3rd ripple
Long relation, the wavelength for making three light sources is in incremental trend；Or between the first wave length, second wave length, the 3rd wavelength
With first wave length>Second wave length>The relation of 3rd wavelength, the trend for making the wavelength of three light sources taper off.
Preferably, the 3rd combined light source can send the discrete monochromatic light of one or more kinds of wavelength, and
The discrete monochromatic wavelength that one combined light source, the second combined light source, the 3rd combined light source can be sent is unequal.
Preferably, the exit direction of the 3rd combined light source is provided with the 3rd collimation lens, the 3rd combined light source
On the photocentre of the 3rd collimation lens.
Preferably, first incident angle is 45 degree；
And/or, second incident angle is 45 degree.
Preferably, the 3rd incident angle is 45 degree.
Speculum in light source of the present invention mainly realizes light path folding, space is saved, particularly in micro- multispectral imaging
Application in.Occupy discounting for space, speculum can be omitted, and main optical path is straightened.
A kind of multiband light spectrum imaging method, including：
According to monochromatic light quantity to be exposed, the exposure cycle of camera, each exposure are detected according to the timing settings of exposure
In cycle, correspondence exposure period, an a kind of monochromatic light of exposure period correspondence, two neighboring exposure are designed for each monochromatic light
Retaining a time interval between light time section is used for data transfer with preserving；
In the corresponding monochromatic light of each exposure period, monochromatic light is lit before the starting point of exposure period, and is continued
It is off after terminating to exposure period, adjacent two kinds of monochromatic time for exposure are not overlapping.
A kind of microscope, including imaging source as described above, or use imaging method as described above.
A kind of multiband spectrum imaging source and imaging method that the present invention is provided, with low cost, the effect of fast imaging
Really, it is possible to achieve the multiband multispectral imaging of the 60 frames/more than second, the requirement of most of medical science multispectral imaging application is met.
Brief description of the drawings
The present invention is described in further detail below according to drawings and examples.
Fig. 1 is a kind of structural representation of multiband spectrum imaging source described in an embodiment.
Fig. 2 is LED (or semiconductor laser) luminescence chip layout structure schematic diagram of combined light source described in an embodiment.
Fig. 3 is a kind of time diagram of multiband light spectrum imaging method described in an embodiment.
Fig. 4 is a kind of structural representation of multiband spectrum imaging source described in another embodiment.
Embodiment
As Figure 1-4, the embodiment of the present invention provides a kind of multiband spectrum imaging source, including：
First combined light source 11, can at least send the discrete monochromatic light of first wave length, and accurate through the first collimating mirror 31
With the minute surface of the first incident angle directive speculum 50 after straight；
Speculum 50, for receiving the incident light of first combined light source 11 and reflecting away, the first combination light
The first light path is formed between source 11 and the speculum 50；
First semi-transparent semi-reflecting eyeglass 21, is located at the first light path between first combined light source 11 and the speculum 50
On, the first semi-transparent semi-reflecting eyeglass 21 includes mutually deviating from and parallel the first minute surface and the second minute surface, first combination
Light source 11 is with the first minute surface of the first semi-transparent semi-reflecting eyeglass 21 described in the first incident angle directive；
Second combined light source 12, can at least send the discrete monochromatic light of second wave length, and the second combined light source 12 is located at institute
The position beyond the first light path is stated, and second combined light source 12 is semi-transparent semi-reflecting with described in the second incident angle directive first
Second minute surface of eyeglass 21；
Wherein, the first wave length and the second wave length are unequal, and first incident angle 30-60 degree it
Between, second incident angle is between 30-60 degree.
Preferably, the first collimation lens 31, first combination are provided with the exit direction of first combined light source 11
Light source 11 is located on the photocentre of first collimation lens 31；
The exit direction of second combined light source 12 is provided with the second collimation lens 32, second combined light source 12
In on the photocentre of second collimation lens 32.
When the first combined light source 11, the second combined light source 12 are spot light, by the way of collimation lens, to diverging
Spot light is collimated, and the light source after collimation is entered back into light path.
Preferably, first combined light source 11 and second combined light source 12 can send one or more kinds of ripples
Long discrete monochromatic light, and the discrete monochromatic wavelength that the first combined light source 11, the second combined light source 12 can be sent is not
It is equal.Specifically, as shown in figure 3, setting the different LED of multiple wavelength by the way of array on the first combined light source 11
Between (or semiconductor laser) luminescence chip, and multiple LED luminescence chips by the way of in parallel, and each LED lights
Chip uses independent control.
Preferably, the described first semi-transparent semi-reflecting eyeglass is dichroic color filter piece, or the first semi-transparent semi-reflecting eyeglass
The metal film reflected for fractional transmission part.Wherein dichroic filter (also known as dichronic mirror) is widely used in fluorescence analysis light
Source and the emission spectrum of fluorogen are separated, and can avoid interference with the progress of analysis.In dichroism filter plate, when the fluorescence of long wave
It is through and the shorter exciting light of wavelength is by high reflection.
Preferably, the multiband spectrum imaging source that the present invention is provided, in addition to：
Second semi-transparent semi-reflecting eyeglass 22, is located at the light path between the described first semi-transparent semi-reflecting eyeglass 21 and the speculum 50
On, the second semi-transparent semi-reflecting eyeglass 22 includes mutually deviating from and the 3rd parallel minute surface and the 4th minute surface, first combination
Light source 11 is with the 3rd minute surface of the second semi-transparent semi-reflecting eyeglass 22 described in the 3rd incident angle directive；
3rd combined light source 13, can at least send the discrete monochromatic light of the 3rd wavelength, the 3rd combined light source 13 with
4th minute surface of the second semi-transparent semi-reflecting eyeglass 22 described in 3rd incident angle directive；
Wherein, the 3rd wavelength and the first wave length, second wave length are unequal, and the 3rd incident angle is in 30-60 degree
Between.
Preferably, there is first wave length between the first wave length, second wave length, the 3rd wavelength<Second wave length<3rd ripple
Long relation, the wavelength for making three light sources is in incremental trend；Or between the first wave length, second wave length, the 3rd wavelength
With first wave length>Second wave length>The relation of 3rd wavelength, the trend for making the wavelength of three light sources taper off.
Preferably, the 3rd combined light source 13 can send the discrete monochromatic light of one or more kinds of wavelength, and
The discrete monochromatic wavelength that first combined light source 11, the second combined light source 12, the 3rd combined light source 13 can be sent not phase
Deng.Specifically refer to the embodiment requirement of the first combined light source 11 and the second combined light source 12.
Preferably, the exit direction of the 3rd combined light source 13 is provided with the 3rd collimation lens 33, the 3rd combination
Light source 13 is located on the photocentre of the 3rd collimation lens 33.
Preferably, first incident angle is 45 degree；
Second incident angle is 45 degree.
Preferably, the 3rd incident angle is 45 degree.
Accompanying drawing 1 is microscope illumination multispectral light source light path proposed by the present invention.The multiband spectrum imaging of the present embodiment
Light source, (or even can also have the 4th combination light using the first combined light source 11, the second combined light source 12, the 3rd combined light source 13
Source, the 5th combined light source), they are collimated into parallel by the first collimating mirror 31, the second collimating mirror 32, the 3rd collimating mirror 33 etc. respectively
Light.The light that first combined light source 11 is sent reaches the first semi-transparent semi-reflecting eyeglass 21 after being collimated through the first collimating mirror 31 (dichroism is filtered
Light microscopic), the light that the second combined light source 12 is sent also reaches the another of the first semi-transparent semi-reflecting eyeglass 21 after being collimated through the second collimating mirror 32
Simultaneously, it can be sent during the principle of the first semi-transparent semi-reflecting eyeglass 21 (dichroic color filter) selection through the first combined light source 11
Light, the light that the second combined light source 12 of reflection is sent.The collimated light of such combined light source 12 of first combined light source 11 and second will be arrived
Up to the second semi-transparent semi-reflecting eyeglass 22 (dichroic color filter), and the light that the 3rd combined light source 13 is sent is collimated through light source collimating mirror 3
The another side of the second semi-transparent semi-reflecting eyeglass 22 will be reached afterwards, and first group can be passed through during the principle that the second semi-transparent semi-reflecting eyeglass 22 is selected
The light that closing light source 11, the second combined light source 12 are sent, the light that the 3rd combined light source 13 of reflection is sent, such first combined light source
11st, the second combined light source 12, the light of the 3rd combined light source 13 collimation can reach speculum, and condenser illumination is entered after reflection and is treated
The sample of imaging.
By the first combined light source 11, the second combined light source 12, the during the simple selection that multiple combined light sources are arranged
Three combined light sources 13 are arranged to the mode of decreasing wavelengths (increasing) successively, and such dichroic color filter can just be grown up with simple selection
The dichroic color filter that (short) wavelength passes through, by wavelength selection reflect and through wavelength centre.
Dichroic color filter can be replaced with part through the Metal film reflector mirror with part reflection, but so light energy meeting
Loss is larger.
It is such to arrange the light source more than 3, but because the limitation of Microscopy spatial, and light are filtered by dichroism
Light microscopic light loss of energy, the number of light source is always limited.
For further increase imaging band number, we are innovated on each combined light source.In view of LED/light source
Luminescence chip volume can be with very little, and much smaller than the volume of traditional Halogen lamp LED, we select the luminous core of multiple monochromatic small sizes
Piece, is arranged closely in around light source center, can at most arrange 1-9 monochromatic LED luminescence chip, and Fig. 3 illustrates four
The arrangement of individual LED chip.
So assume that we have used N number of light source, each light source arranges the chip of M monochrome different wave length, we just can be with
Realize the multispectral imaging of the N number of wave bands of M x.
The present embodiment also provides a kind of multiband light spectrum imaging method, including：
According to monochromatic light (wave band) quantity to be exposed, the exposure cycle of camera is detected according to the timing settings of exposure, often
In individual exposure cycle, correspondence exposure period is designed for each monochromatic light, an a kind of monochromatic light of exposure period correspondence is adjacent
Retaining a time interval between two exposure periods is used for the transmission and preservation of data；
In the corresponding monochromatic light of each exposure period, monochromatic light is lit before the starting point of exposure period, and is continued
It is off after terminating to exposure period, adjacent two kinds of monochromatic time for exposure are not overlapping.
The process of imaging is such：
The first row of accompanying drawing 3 represents the sequential of camera exposure, and high level represents camera in exposure.The second to four rows represent each
The sequential that LED (or semiconductor laser) is lighted, it is luminous that high level represents that LED is lit.
Illustrate the process of multispectral imaging by taking four LED (four wave bands) as an example.
LED1 is lit before camera exposure, and is persistently illuminated to after the completion of camera exposure and is just extinguished, now camera exposure
The image of collection is a spectrum picture of the wavelength of monochromatic LED 1, is immediately stored.
Before second of exposure of camera, LED2 is lit before camera exposure, and is persistently illuminated to after the completion of camera exposure
Just extinguish, the image of now camera exposure collection is a spectrum picture of the wavelength of monochromatic LED 2, is immediately stored.
Before camera third time exposes, LED3 is lit before camera exposure, and is persistently illuminated to after the completion of camera exposure
Just extinguish, the image of now camera exposure collection is a spectrum picture of the wavelength of monochromatic LED 4, is immediately stored.
Before the 4th exposure of camera, LED4 is lit before camera exposure, and is persistently illuminated to after the completion of camera exposure
Just extinguish, the image of now camera exposure collection is a spectrum picture of the wavelength of monochromatic LED 4, is immediately stored.
The spectrum picture of four storages together form the four band spectrums imaging of observed sample.
In the present embodiment, monochromatic LED luminescent device and corresponding thereto thin using required multiple different wave lengths
Film interference dichroic filter is combined to form lighting source, and circuit and camera acquisition image are controlled by appropriate electronic synchronizer
It is synchronous to light each LED/light source successively, the image of different wavelength is obtained by camera acquisition.
So we complete the collection of four wavelength images in the same visual field.
Number of wavelengths increase simply needs to increase the number of times of exposure and collection image, and synchronous camera and light source are very heavy herein
Want.
Accompanying drawing 4 is one of specific embodiment of the invention.
The first combined light source 11 is employed as blue (470nm), green (530nm), red (630nm) three-color LED are integrated herein
LED, this three color and the photosensitive correspondence of human eye cone cell be easy to implement colour imaging, while be the discrete monochromatic spectrum in arrowband, full
Sufficient Lambert law, is conducive to quantitative analysis, while there is off-the-shelf in this LED market.Second combined light source 12 employs 570nm's
Monochromatic LED, the dyestuff of cell feulgen's stain absorbs very strong on this wavelength.
Because in blue (470nm), green (530nm), red (630nm) three color, blue (470nm), green (530nm) wavelength are less than
570nm, red (630nm) is more than 570nm, therefore present embodiment employ semi-transparent semi-reflecting (50% pass through, 50% reflection) without
It is dichroic color filter.Although have lost 50% light energy, because LED luminous intensities are very big, actual answer still can be met
With needs.
Using present embodiment, four wave bands that we realize 470nm, 530nm, 570nm and 630nm are micro- multispectral fast
Rapid-result picture；By related analytical technology, realize Pasteur and Fu Ergen redye under the conditions of cell DNA ploidy analysis, meet
The demand of the analysis of DNA ploidy body and TBS cell analysis is realized on a slide simultaneously.
Finally it should be noted that：The preferred embodiment of invention is the foregoing is only, invention is not limited to, although
Invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, it still can be to preceding
State the technical scheme described in each embodiment to modify, or equivalent substitution is carried out to which part technical characteristic.It is all to send out
Within bright spirit and principle, any modification, equivalent substitution and improvements made etc., should be included in invention protection domain it
It is interior.
Claims (10)
1. a kind of multiband spectrum imaging source, including：
First combined light source (11), can at least send the discrete monochromatic light of first wave length, and accurate through the first collimating mirror (31)
With the minute surface of the first incident angle directive speculum (50) after straight；
Speculum (50), for receiving the incident light of first combined light source (11) and reflecting away, the first combination light
The first light path is formed between source (11) and the speculum (50)；
First semi-transparent semi-reflecting eyeglass (21), is located at the first light between first combined light source (11) and the speculum (50)
Lu Shang, the first semi-transparent semi-reflecting eyeglass (21) includes mutually deviating from and parallel the first minute surface and the second minute surface, described first
Combined light source (11) is with the first minute surface of the described in the first incident angle directive first semi-transparent semi-reflecting eyeglass (21)；
Second combined light source (12), can at least send the discrete monochromatic light of second wave length, and the second combined light source (12) is located at institute
State the position beyond the first light path, and second combined light source (12) is incident with second after collimating mirror (32) is collimated through the
Second minute surface of the first semi-transparent semi-reflecting eyeglass (21) described in angle directive；
Wherein, the first wave length and the second wave length are unequal, and first incident angle is between 30-60 degree,
Second incident angle is between 30-60 degree, it is preferable that first incident angle is 45 degree, it is preferable that described second enters
Firing angle degree is 45 degree.
2. a kind of multiband spectrum imaging source as claimed in claim 1, it is characterised in that
The first collimation lens (31), first combined light source (11) are provided with the exit direction of first combined light source (11)
On the photocentre of first collimation lens (31)；
And/or, the second collimation lens (32), second combination are provided with the exit direction of second combined light source (12)
Light source (12) is located on the photocentre of second collimation lens (32).
3. a kind of multiband spectrum imaging source as claimed in claim 1, it is characterised in that
First combined light source (11) and/or second combined light source (12) can send one or more kinds of wavelength
Discrete monochromatic light, and the discrete monochromatic wavelength that the first combined light source (11), the second combined light source (12) can be sent is not
It is equal.
4. a kind of multiband spectrum imaging source as described in one of claim 1-3, it is characterised in that
The first semi-transparent semi-reflecting eyeglass (21) is dichroic color filter piece, or the first semi-transparent semi-reflecting eyeglass (21) is portion
Divide the metal film of transmissive portion reflection.
5. a kind of multiband spectrum imaging source as described in one of claim 1-4, it is characterised in that also include：
Second semi-transparent semi-reflecting eyeglass (22), is located at the light between the described first semi-transparent semi-reflecting eyeglass (21) and the speculum (50)
Lu Shang, the second semi-transparent semi-reflecting eyeglass (22) includes mutually deviating from and the 3rd parallel minute surface and the 4th minute surface, and described first
Combined light source (11) is with the 3rd minute surface of the described in the 3rd incident angle directive second semi-transparent semi-reflecting eyeglass (22)；
3rd combined light source (13), can at least send the discrete monochromatic light of the 3rd wavelength, the 3rd combined light source is with the 3rd
4th minute surface of the second semi-transparent semi-reflecting eyeglass (22) described in incident angle directive；
Wherein, the 3rd wavelength and the first wave length, second wave length are unequal, the 3rd incident angle between 30-60 degree,
Preferably, the 3rd incident angle is 45 degree.
6. a kind of multiband spectrum imaging source as claimed in claim 5, it is characterised in that
There is first wave length between the first wave length, second wave length, the 3rd wavelength<Second wave length<The relation of 3rd wavelength, makes
The wavelength of three light sources is in incremental trend；Or there is first wave length between the first wave length, second wave length, the 3rd wavelength
>Second wave length>The relation of 3rd wavelength, the trend for making the wavelength of three light sources taper off.
7. a kind of multiband spectrum imaging source as claimed in claim 5, it is characterised in that
3rd combined light source (13) can send the discrete monochromatic light of one or more kinds of wavelength, and the first combination light
The discrete monochromatic wavelength that source (11), the second combined light source (12), the 3rd combined light source (13) can be sent is unequal.
8. a kind of multiband spectrum imaging source as claimed in claim 5, it is characterised in that
The 3rd collimation lens (33), the 3rd combined light source are provided with the exit direction of the 3rd combined light source (13)
(13) on the photocentre of the 3rd collimation lens (33).
9. a kind of multiband light spectrum imaging method, usage right requires the multiband spectrum imaging source described in one of 1-8, bag
Include：
According to monochromatic light quantity to be exposed, detected according to the timing settings of exposure in camera exposure cycle, each exposure cycle,
For each monochromatic light design correspondence exposure period, an a kind of monochromatic light of exposure period correspondence, two neighboring exposure period
Between retain a time interval；
In the corresponding monochromatic light of each exposure period, monochromatic light is lit before the starting point of exposure period, and continues to exposure
Light time section is off after terminating, and adjacent two kinds of monochromatic time for exposure are not overlapping.
10. a kind of microscope, it is characterised in that including the imaging source described in one of claim 1-8, or using such as right
It is required that the imaging method described in 9.
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| CN201710045509.1A CN107044886A (en) | 2017-01-20 | 2017-01-20 | A kind of multiband spectrum imaging source, imaging method and microscope |
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| CN110749282A (en) * | 2019-11-04 | 2020-02-04 | 和卓生物科技（上海）有限公司 | Suction head calibration equipment |
| CN111308289A (en) * | 2020-03-06 | 2020-06-19 | 西安交通大学 | Partial discharge multispectral weak light detection device and method |
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