CN104570311A - Fluorescence microscope based on multi-spectral LED light source - Google Patents
Fluorescence microscope based on multi-spectral LED light source Download PDFInfo
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- CN104570311A CN104570311A CN201410830190.XA CN201410830190A CN104570311A CN 104570311 A CN104570311 A CN 104570311A CN 201410830190 A CN201410830190 A CN 201410830190A CN 104570311 A CN104570311 A CN 104570311A
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- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
Abstract
The invention discloses a fluorescence microscope based on a multi-spectral LED light source. The fluorescence microscope comprises a fluorescence microscope body and the light source, wherein the light source comprises a plurality of LED chips, wave bands of the LED chips comprise wave bands with multiple different central wavelengths, and the kind number of the wave bands with multiple different central wavelengths is equal to or smaller than the number of the LED chips; the LED chips are arranged on a line layer which is arranged on a substrate, the line layer is further provided with a plurality of pins, the number of the pins corresponds to that of the LED chips, an electrode at one end of each LED chip is connected in series and/or connected in parallel, and electrodes at the other ends of the LED chips are led out by the pins. Compared with the prior art, the cost is reduced, more importantly, different wavelengths of one set of light source can be obtained through adjustment as required, so that use variability of the light source of the fluorescence microscope is extended, and the fluorescence microscope can have the multi-spectral LED light source.
Description
Technical field
The present invention relates to a kind of fluorescent microscope, be specifically related to a kind of fluorescent microscope based on multispectral section of LED light source.
Background technology
Fluorescent microscope, the narrow band light mainly sent with certain light source or wide spectrum optical, the narrow band light (as 365nm ultraviolet light or 420nm purplish blue light) of certain wavelength is produced as exciting light through color filter array, irradiate by the sample of fluorochrome label, fluorescent material in excited sample launches the fluorescence of various different colours, after another fluorescence color filter array, then observed by the amplification of object lens and eyepiece.Like this strong under backing scape, also easily recognize even if fluorescence is very faint, highly sensitive, can be used for studying the absorption of intracellular matter, transport, the distribution of chemical substance and location etc., is the desirable instrument carrying out the research work such as biology, cytology, oncology, science of heredity, immunology.
The essential structure of fluorescent microscope is added some annexes (as fluorescence light source, exciting filter disc, dichromatic beam separation vessel and block filter glass etc.) basis by ordinary optical microscope forms.Fluorescence light source generally adopts ultrahigh pressure mercury lamp (50 ~ 200W), it can send the light of various wavelength, but often kind of fluorescent material has an excitation wavelength producing most hyperfluorescenceZeng Yongminggaoyingguang, so need add with optical filter (generally having ultraviolet, purple, blueness and green to excite filter disc), only make the exciting light of certain wavelength through being irradiated on sample, and other light are all sponged.Often kind of material is excited after illumination penetrates, and launches the visible fluorescence longer compared with illumination wavelength in very short time.Fluorescence has selectivity, generally all weak than exciting light, for observing single-minded fluorescence, needs to add cutoff filter after object lens.Its effect has be absorb and stop exciting light enter eyepiece in order to avoid interference fluorescence and damage eyes at two: one, two be select and allow special fluorescence through, show single-minded fluorescent color.Two kinds of optical filters must select with the use of.
Current world-leading traditional fluorescence microscope producer, comprise Olympus, Nikon, Zeiss, Lycra etc., the conventional light source such as main employing xenon lamp, mercury lamp, Metal halogen lamp are as excitation source, by being equipped with the mode of different fluorescence excitation module, realize the fluorescence excitation to the fluorescent dye of different peak excitation wavelength, the conventional light source life-span is shorter, mercury lamp typical life 200 hours, in 2000 hours Metal halogen lamp life-spans, equipment is maintainable poor.Eight wave band fluorescence excitations are accomplished at most by current Olympus, Nikon company, as Olympus IX83, BX63 series microscope, Nikon ECLIPSE Ti series microscope, primary structure is equipped with white light source (as mercury lamp, Metal halogen lamp, LED etc.), in order to obtain multiple different fluorescent samples, fluorescent microscope generally needs to overlap color filter system more, arranges multiplely to excite position, hole, for arranging optical filter, by the light output realizing multiple different excitation wavelength that filters.This series microscope is powerful, there is auto-focusing, the support of multilevel extension design multiport, possess the functions such as differential interference (DIC), difference, dark-field imaging function, just because of being integrated with many functions, therefore expensive (3-5 ten thousand dollars), the colleges and universities of minority research funding abundance and scientific research institutions are only had to have the ability to buy.And what is more important, the conventional light source such as xenon lamp, mercury lamp, Metal halogen lamp are as excitation source, and its wavelength is fixing after microscope manufacture completes, and are the wavelength that cannot remove to control and regulate excitation source as required in follow-up use.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of low cost and the adjustable fluorescent microscope based on multispectral section of LED light source of optical source wavelength.
In order to achieve the above object, technical scheme of the present invention is as follows:
Based on the fluorescent microscope of multispectral section of LED light source, comprise fluorescent microscope body and the light source of light is provided to fluorescent microscope body, this light source comprises multiple LED chip and/or LED chip group, the wave band of multiple different centre wavelength is contained in the wave band that this LED chip and/or LED chip group have, its kind quantity is equal to or less than the quantity of this LED chip and/or LED chip group, this LED chip and/or LED chip group are also arranged on line layer, this line layer is arranged on substrate, this line layer also has the multiple pins corresponding with the quantity of LED chip and/or LED chip group, one end electrode of this LED chip and/or LED chip group is connected and/or is connected in parallel, relative other end electrode is drawn by pin.
The excitation source of the present invention to fluorescent microscope has made improvement, first instead of xenon lamp of the prior art, mercury lamp, Metal halogen lamp equal excitation light source with LED light source, reduces the cost that light source uses, secondly, in multiple LED chip and/or LED chip group, it is provided with the wave band of multiple different centre wavelength, each there is different centre wavelength LED chip or LED chip group by be located on line layer with LED chip or LED chip group relative to individual pin draw, one or more LED chip or LED chip group can connected sometime by this pin, all the other LED chips be not switched on or LED chip group are in closed condition, now, the LED chip that the light that whole LED light source exports is switched on power by these or the light that LED chip group sends synthesize, the light source of what wavelength is needed only to need to connect the LED chip or LED chip group accordingly with different centre wavelength, thus achieve the regulable control of the centre wavelength to light source.
Therefore, the present invention compared with prior art, which not only reduces cost, what is more important, also achieve the object to a set of light source adjusting as required the light source of different wave length, thus extend the polytrope of light source on using on fluorescent microscope, make fluorescent microscope can have the LED light source of multispectral section.
Simultaneously, this fluorescent microscope based on multispectral section of LED light source has LED chip or the LED chip group of multiple different centre wavelength, thus the light outgoing of different wave length can be obtained as required, therefore, exciter filter in optical filter box is optional components, if LED light bandwidth is enough narrow, without exciter filter, exciting light can be directly incident in microscopic system.
On the basis of technique scheme, the present invention can also do following improvement:
As preferred scheme, above-mentioned LED chip and/or LED chip group are arranged in the form of an array.
Adopt above-mentioned preferred scheme, LED chip and/or LED chip group are arranged in the form of an array, make the bright dipping of light source can be more even.
As preferred scheme, a termination electrode of above-mentioned LED chip and/or LED chip group is that common anode pole connects, and multiple pin is that common cathode connects; Or a termination electrode of LED chip and/or LED chip group is that common cathode connects, multiple pin is that common anode pole connects.
Adopt above-mentioned preferred scheme, two kinds of optional electrical connection scheme of LED chip and/or LED chip group can be formed.
As preferred scheme, in above-mentioned LED chip and/or LED chip group, there is the LED chip of identical central wavelength and/or LED chip group in parallel or be connected in series, LED chip and/or the LED chip group with different centre wavelength are only connected in parallel.
Adopt above-mentioned preferred scheme, the LED chip of identical central wavelength and/or the connection of LED chip group are put together and are once connected or gradation connection, the LED chip of different centre wavelength and/or LED chip component time are connected, thus can so that the LED chip of identical central wavelength and/or the LED chip of LED chip group and different centre wavelength and/or the connection of LED chip group are made a distinction.
As preferred scheme, above-mentioned light source is also provided with control circuit, and one controls the connecting and disconnecting of the circuit of a LED chip or LED chip group, or once controls the connecting and disconnecting of the circuit of multiple LED chip and/or LED chip group.
Adopt above-mentioned preferred scheme, automatically the connection of LED chip and/or LED chip group can be controlled by control circuit, this control circuit can be independently circuit, also can be by the integrated circuit of microcomputerized control, the LED chip of each different centre wavelength or LED chip group are connected on the different output pin of control circuit by the independently pin be located on line layer, realize independently controlling different LED chip or the real-time of LED chip group with this, one or more LED chip or LED chip group can connected sometime, remaining LED chip or LED chip group are in closed condition.
As preferred scheme, above-mentioned control circuit is also for controlling the electrical current size of LED chip and/or LED chip group.
Adopt above-mentioned preferred scheme, the adjustment of the brightness to LED chip and/or LED chip group can be realized.
As preferred scheme, above-mentioned control circuit also controls the intrinsic optical filter of fluorescent microscope according to the conducting of the pin of certain LED chip corresponding or LED chip group and is adjusted to corresponding position.
Adopt above-mentioned preferred scheme, after the wavelength adjustment of light source, optical filter also needs to adjust to relative position, all manual adjustments in the past, can control optical filter by control circuit according to the conducting of pin now automatically to regulate, can be specifically to drive motor signal, drive motor again automatic traction optical filter box rotates certain stroke.
As preferred scheme, between above-mentioned line layer and substrate, be provided with insulation course.
Adopt above-mentioned preferred scheme, the insulation between line layer and substrate can be formed.
Accompanying drawing explanation
Fig. 1 is the structural representation of the fluorescent microscope based on multispectral section of LED light source of the present invention.
Fig. 2 is the structural representation based on LED light source involved in the fluorescent microscope of multispectral section of LED light source of the present invention.
Fig. 3 for of the present invention based on the circuit diagram of LED light source involved in the fluorescent microscope of multispectral section of LED light source.
Wherein, 1. optical filter 26. catoptron 27. mirror 28. detector 29. object lens 30. objective tables launched by light source 11.LED chip 12. pin 13. common cathode 14. substrate 15. line layer 16. insulation course 2. fluorescent microscope body 21. lens 22. catoptron 23. exciter filter 24. dichroic mirror 25..
Embodiment
The preferred embodiment of the present invention is described in detail below in conjunction with accompanying drawing.
In order to reach object of the present invention, as shown in Figure 1-2, in some embodiments of the fluorescent microscope based on multispectral section of LED light source of the present invention, comprise fluorescent microscope body 2 and the light source 1 of light is provided to fluorescent microscope body 2, this fluorescent microscope body 2 comprises lens 21, catoptron 22, exciter filter 23, dichroic mirror 24, launch optical filter 25, catoptron 26, cylinder mirror 27, detector 28, object lens 29 and objective table 30, in some implementations, also difference can be there is therewith in the structure of fluorescent microscope body 2, only provide a kind of fluorescent microscope body 2 of structure herein, its with can know from prior art, will not enumerate at this, as improvement, this light source 1 comprises nine LED chips 11, contain in the wave band that these nine LED chips 11 have nine kinds or be less than the wave band of nine kinds of different centre wavelengths, the centre wavelength scope of nine LED chips 11 contains ultraviolet band to infrared band, this LED chip 11 is also arranged on line layer 15, this line layer 15 is arranged on substrate 14, this line layer 15 also has nine pins 12 corresponding with the quantity of LED chip 11, one end electrode of this LED chip 11 is connected in series or in parallel, also can connect and deposit enforcement by series and parallel connections, other end electrode relative on this LED chip 11 is drawn by pin 12.
The light that light source 1 sends, first carry out beam shaping through two lens 21, the uniform illumination forming collimation is bright, , from the light of lens 21 outgoing, after catoptron 22 reflects, incide exciter filter 23, after filtering, form narrow band light, reflected by dichroic mirror 24, incide object lens 29, focus on through object lens 29, incide on the fluorescent samples of objective table 30, fluorescent samples irradiates through exciting light and produces fluorescence, dichroic mirror 24 is turned back to through object lens 29 along identical light path, fluorescence bands be in dichroic mirror 24 through wave band, after dichroic mirror 24, after launching optical filter 25 and filtering further, after catoptron 26 reflects, tube mirror 27 focuses on the target surface of detector 28, thus acquisition fluoroscopic image.
This has made improvement based on the excitation source of fluorescent microscope to fluorescent microscope of multispectral section of LED light source, first instead of xenon lamp of the prior art, mercury lamp, Metal halogen lamp equal excitation light source with LED light source, reduces the cost that light source uses, secondly, in multiple LED chip and/or LED chip group, it is provided with the wave band of multiple different centre wavelength, each there is different centre wavelength LED chip or LED chip group by be located on line layer with LED chip or LED chip group relative to individual pin draw, one or more LED chip or LED chip group can connected sometime by this pin, all the other LED chips be not switched on or LED chip group are in closed condition, now, the LED chip that the light that whole LED light source exports is switched on power by these or the light that LED chip group sends synthesize, the light source of what wavelength is needed only to need to connect the LED chip or LED chip group accordingly with different centre wavelength, thus achieve the regulable control of the centre wavelength to light source.
Therefore, this fluorescent microscope compared with prior art, which not only reduces cost, what is more important, also achieve the object to a set of light source adjusting as required the light source of different wave length, thus extend the polytrope of light source on using on fluorescent microscope, make fluorescent microscope can have the LED light source of multispectral section.
Simultaneously, this fluorescent microscope based on multispectral section of LED light source has LED chip or the LED chip group of multiple different centre wavelength, thus the light outgoing of different wave length can be obtained as required, therefore, exciter filter in optical filter box is optional components, if LED light bandwidth is enough narrow, without exciter filter, exciting light can be directly incident in microscopic system.
In order to optimize implementation result of the present invention further, as shown in Figure 2, in other embodiments of the fluorescent microscope based on multispectral section of LED light source of the present invention, on the basis of foregoing teachings, above-mentioned LED chip 11 is arranged in the form of an array, and this array format can be circular array or rectangular array etc.Adopt the scheme of this embodiment, LED chip is arranged in the form of an array, make the bright dipping of light source can be more even.
In order to optimize implementation result of the present invention further, as shown in Figure 2, in other embodiments of the fluorescent microscope based on multispectral section of LED light source of the present invention, on the basis of foregoing teachings, one termination electrode of above-mentioned LED chip 11 is that common anode pole connects, and multiple pin 12 is connected by common cathode 13.Alternatively, a termination electrode of LED chip 11 can connect for common cathode, and multiple pin 12 can connect for common anode pole.Adopt the scheme of this embodiment, two kinds of optional electrical connection scheme of LED chip can be formed.
In order to optimize implementation result of the present invention further, in other embodiments of the fluorescent microscope based on multispectral section of LED light source of the present invention, on the basis of foregoing teachings, in above-mentioned LED chip, have the LED chip parallel connection of identical central wavelength or be connected in series, the LED chip with different centre wavelength is only connected in parallel.Adopt the scheme of this embodiment, the connection of the LED chip of identical central wavelength is put together and is once connected or gradation connection, the LED chip gradation of different centre wavelength is connected, thus can so that the connection of the LED chip of the LED chip of identical central wavelength and different centre wavelength made a distinction.
In order to optimize implementation result of the present invention further, in other embodiments of the fluorescent microscope based on multispectral section of LED light source of the present invention, on the basis of foregoing teachings, above-mentioned light source is also provided with control circuit, one controls the connecting and disconnecting of the circuit of a LED chip, or once control the connecting and disconnecting of the circuit of multiple LED chip, namely control circuit has the on-off circuit controlling break-make.Adopt the scheme of this embodiment, automatically the connection of LED chip can be controlled by control circuit, this control circuit can be independently circuit, also can be by the integrated circuit of microcomputerized control, the LED chip of each different centre wavelength is connected on the different output pin of control circuit by the independently pin be located on line layer, independently controlling in real time different LED chip is realized with this, can connect one or more LED chip sometime, remaining LED chip is in closed condition.
In order to optimize implementation result of the present invention further, in other embodiments of the fluorescent microscope based on multispectral section of LED light source of the present invention, on the basis of foregoing teachings, above-mentioned control circuit is also for controlling the electrical current size of LED chip, and namely control circuit also has current control circuit.Adopt the scheme of this embodiment, the adjustment of the brightness to LED chip can be realized.
In order to optimize implementation result of the present invention further, in other embodiments of the fluorescent microscope based on multispectral section of LED light source of the present invention, on the basis of foregoing teachings, above-mentioned control circuit also controls the intrinsic optical filter of fluorescent microscope according to the conducting of the pin of certain LED chip corresponding and is adjusted to corresponding position.Adopt the scheme of this embodiment, after the wavelength adjustment of light source, optical filter also needs to adjust to relative position, all manual adjustments in the past, can control optical filter by control circuit according to the conducting of pin now automatically to regulate, can be specifically to drive motor signal, drive motor again automatic traction optical filter box rotates certain stroke.
In order to optimize implementation result of the present invention further, as shown in Figure 2, in other embodiments of the fluorescent microscope based on multispectral section of LED light source of the present invention, on the basis of foregoing teachings, between above-mentioned line layer 15 and substrate 14, be provided with insulation course 16.Adopt the scheme of this embodiment, the insulation between line layer and substrate can be formed.
In above-mentioned embodiment, substrate 14 can adopt the substrate of high heat conduction; The quantity of LED chip 11 can also be set to minimum two or more than nine etc. as required, and in LED chip 11, some or all of can also with LED chip group substitute, this LED chip group can comprise one or more LED chip further, by the LED chip of integrated difference group number, can realize different adjustment progression, a group can be connected or be connected in parallel into multiple LED chip.
Above-described is only the preferred embodiment of the present invention, it should be pointed out that for the person of ordinary skill of the art, and without departing from the concept of the premise of the invention, can also make some distortion and improvement, these all belong to protection scope of the present invention.
Claims (8)
1. based on the fluorescent microscope of multispectral section of LED light source, comprise fluorescent microscope body and the light source of light is provided to described fluorescent microscope body, it is characterized in that, described light source comprises multiple LED chip and/or LED chip group, the wave band of multiple different centre wavelength is contained in the wave band that described LED chip and/or LED chip group have, its kind quantity is equal to or less than the quantity of described LED chip and/or LED chip group, described LED chip and/or LED chip group are also arranged on line layer, described line layer is arranged on substrate, described line layer also has the multiple pins corresponding with the quantity of described LED chip and/or LED chip group, one end electrode of described LED chip and/or LED chip group is connected and/or is connected in parallel, relative other end electrode is drawn by described pin.
2. the fluorescent microscope based on multispectral section of LED light source according to claim 1, is characterized in that, described LED chip and/or LED chip group are arranged in the form of an array.
3. the fluorescent microscope based on multispectral section of LED light source according to claim 1 and 2, is characterized in that, a described termination electrode of described LED chip and/or LED chip group is that common anode pole connects, and described multiple pin is that common cathode connects; Or a described termination electrode of described LED chip and/or LED chip group is that common cathode connects, described multiple pin is that common anode pole connects.
4. the fluorescent microscope based on multispectral section of LED light source according to claim 1, it is characterized in that, in described LED chip and/or LED chip group, have LED chip and/or the parallel connection of LED chip group of identical central wavelength or be connected in series, LED chip and/or the LED chip group with different centre wavelength are only connected in parallel.
5. the fluorescent microscope based on multispectral section of LED light source according to claim 1, it is characterized in that, described light source is also provided with control circuit, one controls the connecting and disconnecting of the circuit of a described LED chip or LED chip group, or once controls the connecting and disconnecting of the circuit of multiple described LED chip and/or LED chip group.
6. the fluorescent microscope based on multispectral section of LED light source according to claim 5, is characterized in that, described control circuit is also for controlling the electrical current size of described LED chip and/or LED chip group.
7. the fluorescent microscope based on multispectral section of LED light source according to claim 5 or 6, it is characterized in that, described control circuit also controls the intrinsic optical filter of described fluorescent microscope according to the conducting of the pin of corresponding LED chip described in certain or LED chip group and is adjusted to corresponding position.
8. the fluorescent microscope based on multispectral section of LED light source according to claim 1, is characterized in that, is provided with insulation course between described line layer and described substrate.
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| CN201410830190.XA CN104570311A (en) | 2014-12-26 | 2014-12-26 | Fluorescence microscope based on multi-spectral LED light source |
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| CN201410830190.XA CN104570311A (en) | 2014-12-26 | 2014-12-26 | Fluorescence microscope based on multi-spectral LED light source |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108050429A (en) * | 2017-12-12 | 2018-05-18 | 苏州科医世凯半导体技术有限责任公司 | A kind of multispectral light path light source illuminating apparatus altogether of LED |
| CN108761756A (en) * | 2018-07-13 | 2018-11-06 | 广州市明美光电技术有限公司 | Multiband LED fluorescence microscopes |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060232855A1 (en) * | 2005-04-15 | 2006-10-19 | Mitaka Kohki Co., Ltd | Bright-field light source for fluorescence observation and surgical microscope with bright-field light source |
| CN203224665U (en) * | 2013-04-17 | 2013-10-02 | 宁波舜宇仪器有限公司 | LED fluorescence lighting device |
| US20140063797A1 (en) * | 2011-04-15 | 2014-03-06 | Dicon Fiberoptics, Inc. | Multiple wavelength led array illuminator for fluorescence microscopy |
| CN103836387A (en) * | 2012-11-23 | 2014-06-04 | 苏州科医世凯半导体技术有限责任公司 | LED fluorescence excitation light source system |
| CN203732796U (en) * | 2014-01-26 | 2014-07-23 | 中国科学院苏州生物医学工程技术研究所 | Structured light illumination optical system |
-
2014
- 2014-12-26 CN CN201410830190.XA patent/CN104570311A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060232855A1 (en) * | 2005-04-15 | 2006-10-19 | Mitaka Kohki Co., Ltd | Bright-field light source for fluorescence observation and surgical microscope with bright-field light source |
| US20140063797A1 (en) * | 2011-04-15 | 2014-03-06 | Dicon Fiberoptics, Inc. | Multiple wavelength led array illuminator for fluorescence microscopy |
| CN103836387A (en) * | 2012-11-23 | 2014-06-04 | 苏州科医世凯半导体技术有限责任公司 | LED fluorescence excitation light source system |
| CN203224665U (en) * | 2013-04-17 | 2013-10-02 | 宁波舜宇仪器有限公司 | LED fluorescence lighting device |
| CN203732796U (en) * | 2014-01-26 | 2014-07-23 | 中国科学院苏州生物医学工程技术研究所 | Structured light illumination optical system |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108050429A (en) * | 2017-12-12 | 2018-05-18 | 苏州科医世凯半导体技术有限责任公司 | A kind of multispectral light path light source illuminating apparatus altogether of LED |
| CN108761756A (en) * | 2018-07-13 | 2018-11-06 | 广州市明美光电技术有限公司 | Multiband LED fluorescence microscopes |
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