CN203732796U - Structured light illumination optical system - Google Patents
Structured light illumination optical system Download PDFInfo
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- CN203732796U CN203732796U CN201420049633.7U CN201420049633U CN203732796U CN 203732796 U CN203732796 U CN 203732796U CN 201420049633 U CN201420049633 U CN 201420049633U CN 203732796 U CN203732796 U CN 203732796U
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Abstract
The utility model discloses a structured light illumination optical system. The system comprises a computer, a synchronous control system, an ultra-high brightness LED excitation light source, a relay lens group, a compound eye lens array, an intermediate image surface, a dichroscope, a projection objective lens, an objective table, a cylinder lens, a narrow-band optical filter and a photoelectric detector. According to the system of the utility model, the computer is used to control a multi-chip structure of the ultra-high brightness LED excitation light source so that initial stripe light is acquired. Through the relay lens group and the compound eye lens array, the number of the stripes is multiplied. Then the projection objective lens is used to produce images according to certain amplification multiplying power on the illumination objective table so as to form structured light illumination. A mode of electrically controlling a LED chip is used to acquire the structured light illumination. By using a traditional raster and a spatial light modulator, cost is high; the size is large and a stripe phase shift speed is low. By using the system of the utility model, the above disadvantages are abandoned. The system is suitable for rapid real-time three-dimensional imaging research of living biological cells.
Description
Technical field
The utility model relates to Structured Illumination optical system novel in a kind of super-resolution micro-imaging technique, refers to specifically a kind of Structured Illumination microscope imaging optical system based on fly eye lens array, belongs to optical image technology field.
Background technology
Ordinary optical microscope is subject to the restriction of optical diffraction limit, and lateral resolution generally can only reach 200nm, and the about 500nm of longitudinal frame is difficult to meet the Research Requirements of biology at present field to subcellular structure and molecular structure.
Structure light imaging technology is proposed in 1997 first by people such as Neil at first, and it is applied to biology imaging, and resolution can reach 100nm.The light field illumination sample of this utilization particular modulation, by using the information of the never synchronous modulation image extracting data of phase shift algorithm focussing plane, obtain the view data of Structured Illumination micro-imaging, with current existing several super-resolution optical micro imaging methods, as photoactivation localization method (PALM), random optics Reconstruction Method (STORM) and stimulated emission loss method (STED) etc. are compared, there is image taking speed very fast, simple in structure, be suitable for the advantages such as biological tissue Real Time Observation.
Structured Illumination is micro-the earliest by adding a sinusoidal grating to realize in illumination path, grating pattern is projected to and on sample, forms Structured Illumination, grating is installed on a piezoelectric ceramics and realizes stepping by piezoelectric ceramics controller, in 1/3 of each moving grating cycle, be equivalent to grating pattern phase shift 2 π/3.Three phase shifts (0,2 π/3,4 π/3) obtain three width source images of sample, by a simple algorithm, can obtain the tomographic map of sample; And by another one algorithm, can by with grating fringe vertical direction on transverse spatial resolution double.Obtain in the system of Structured Illumination with grating at these, must obtain the source images under different phase shifts by moving grating, therefore this mechanical mobile device can lower the stability of system.
Fig. 1 is traditional Structured Illumination micro imaging system fundamental diagram.
As shown in Figure 1: in structure, adopt the light path of the Structured Illumination micro imaging system of grating mainly to be formed by grating, lighting source, lens combination, CCD and synchronous control system at present.The light that light source sends, through collimation, produces structured light through grating, illumination object under test surface, and the fluorescence imaging that lens combination is sent body surface is in CCD plane.Utilize piezo-electric device that the movement (corresponding to phase shifts) of grating is synchronizeed with the speed of CCD document image, after piece image has recorded, grating moves a displacement, the corresponding image in the new position of record.The imagery exploitation special algorithm of record is reconstructed, obtains the two dimensional image of tomographic map and ultrahigh resolution.This class light path, simple in structure, image taking speed is fast.But, there is the problems such as time precision in the precision, synchronizing process of moving process meta phase, signal to noise ratio (S/N ratio) be low.
Utility model patent 200810071654.8 has proposed a kind ofly to cut tomographic image acquisition methods based on two-dimensional modulation technology, the method utilizes two dimensional spatial light modulator as the grating in the replacement such as digital micromirror DMD, penetration liquid crystal LCD, reflective type silicon-based liquid crystal LCOS traditional structure light microscope, illumination is carried out to two-dimensional modulation, thereby produce the modulation pattern of one dimension out of phase.Utility model patent 201110448980.8 has proposed a kind of high-speed structures light optics microscopic system and method based on Digital Micromirror Device, has realized high speed imaging and high-light-energy utilization factor.But there is the shortcomings such as cost is high, image taking speed is still restricted, system bulk is large in spatial light modulator.So far, all micro-technology of Structured Illumination that relate to, are mainly based on above two kinds of modes.
Utility model content
For solving the deficiencies in the prior art, the purpose of this utility model is to provide a kind of the automatically controlled luminous of LED chip of passing through, the ns level switch speed of implementation structure striations, the Structured Illumination optical system of the micro-imaging capability of acquisition high-speed structures light display.
For achieving the above object, the utility model is to realize by following technical scheme:
A kind of Structured Illumination optical system, is characterized in that, comprising:
There is the LED excitation source of multiple chip structures;
Be communicated with LED excitation source and independent control the bright dark of each chip and then control whole LED excitation source the computing machine that forms bright dark fringe light;
Be positioned at the relay lens group in the transmitting light path of bright dark fringe light;
Comprise multiple fly's-eye lenses unit, and each fly's-eye lens unit all receives through the bright dark fringe light of relay lens group and carries out imaging with the multiple chip structures to LED excitation source, thereby in intermediate image plane, form the fly eye lens array of the structural light stripes of number multiplication;
For the intermediate image plane of reflection configuration striations;
Be positioned at the dichroic mirror for transmittance structure striations in structural light stripes transmitting light path;
Receive the projection objective that sees through the structural light stripes of dichroic mirror and it is amplified to rear projection according to certain multiplying power;
Be arranged on objective table, after the structural light stripes of reception projection objective projection, form Structured Illumination and excite the illumination on target that produces fluorescence;
Be positioned in the light path of described fluorescence after dichroic mirror reflects, for the cylinder mirror of fluorescence described in transmission;
For the narrow band pass filter that the fluorescence that sees through cylinder mirror is filtered;
For receiving the photodetector of described fluorescence.
Further, also comprise a synchronous control system, described synchronous control system is communicated with described computing machine and LED excitation source respectively.
And described LED excitation source and intermediate image plane, intermediate image plane and illumination on target, illumination on target and photodetector imaging surface are all in conjugated structure position.
Further, described LED excitation source comprises LED substrate, organizes LED chip more, positive source and power cathode, described many groups LED chip, positive source and power cathode is provided with on LED substrate, described many groups LED chip is vertically set up in parallel by group, and a wherein electrode tip of described many groups LED chip is all communicated with the electrical connection of the formation common anode utmost point with positive source, and the another one electrode tip of described multiple LED chips is independently drawn by being communicated with computing machine with LED chip equivalent multi-group power negative pole by light path layer.
Or, described LED excitation source comprises LED substrate, organizes LED chip more, positive source and power cathode, described many groups LED chip, positive source and power cathode is provided with on LED substrate, described many groups LED chip is vertically set up in parallel by group, and a wherein electrode tip of described many groups LED chip is all communicated with the electrical connection of formation common cathode with power cathode, and the another one electrode tip of described multiple LED chips is independently drawn by being communicated with computing machine with LED chip equivalent multi-group power is anodal by light path layer.
In addition, described LED substrate is metal substrate or ceramic substrate.And the luminous wave band of described many groups LED chip is identical or different.
Further, described multiple fly's-eye lenses unit is arranged in the mode of matrix.
Wherein, described fly's-eye lens unit is one side fly's-eye lens unit or two-face fly-eye lens unit.
And the wherein one side of described one side fly's-eye lens unit is plane, another side is free form surface or sphere.
The beneficial effects of the utility model are: a kind of novel Structured Illumination optical system the utility model proposes, by fly eye lens array being introduced to Structured Illumination microtechnic, greatly reduce system bulk and cost, and utilize the ns level light switching capability of LED chip, the high speed that has obtained structural light stripes is switched and phase shift ability, the impact of the optical illumination imaging system of effectively having avoided adopting traditional raster formula structure and spatial light modulator on system stability, is more suitable for the real time three-dimensional imaging research of living body biological cell.
Brief description of the drawings
Fig. 1 is traditional Structured Illumination micro imaging system fundamental diagram;
Fig. 2 is the structural representation of the utility model one embodiment;
Fig. 3 is the structural representation of LED excitation source described in the utility model;
Fig. 4 is the planar structure schematic diagram of fly's-eye lens described in the utility model unit;
Fig. 5 is the side structure schematic diagram of fly's-eye lens described in the utility model unit;
Fig. 6 is the structural light stripes schematic diagram that intermediate image plane described in the utility model forms;
Fig. 7 is the structural light stripes schematic diagram of illumination on target described in the utility model.
In figure, main Reference numeral implication is:
1, computing machine 2, synchronous control system 3, LED excitation source
4, fly eye lens array 41, fly's-eye lens unit 5, relay lens group
6, intermediate image plane 7, dichroic mirror 8, projection objective
9, illumination on target 10, cylinder mirror 11, narrow band pass filter
12, photodetector 13, LED chip 14, LED substrate
15, positive source 16, power cathode.
Embodiment
Below in conjunction with the drawings and specific embodiments, the utility model is carried out to concrete introduction.
Fig. 2 is the structural representation of the utility model one embodiment.
As shown in Figure 2: a kind of Structured Illumination optical system, comprising: the LED excitation source 3 with multiple chip structures; Be communicated with LED excitation source 3 and independent control the bright dark of each chip and then control whole LED excitation source 3 computing machine 1 that forms bright dark fringe light; Be positioned at the relay lens group 5 in the transmitting light path of bright dark fringe light; Comprise multiple fly's-eye lenses unit 41, and each fly's-eye lens unit 41 all receives through the bright dark fringe light of relay lens group 5 and carries out imaging with the multiple chip structures to LED excitation source 3, thereby in intermediate image plane 6, form the fly eye lens array 4 of the structural light stripes of number multiplication; For the intermediate image plane 6 of reflection configuration striations; Be positioned at the dichroic mirror 7 for transmittance structure striations in structural light stripes transmitting light path; Receive the projection objective 8 that sees through the structural light stripes of dichroic mirror 7 and it is amplified to rear projection according to certain multiplying power; Be arranged on objective table, after the structural light stripes of reception projection objective 8 projections, form Structured Illumination and excite the illumination on target 9 that produces fluorescence; Be positioned in the light path of described fluorescence after dichroic mirror 7 reflections, for the cylinder mirror 10 of fluorescence described in transmission; For the narrow band pass filter 11 that the fluorescence that sees through cylinder mirror 10 is filtered; And for receiving the photodetector 12 of described fluorescence.Also comprise a synchronous control system 2, described synchronous control system 2 is communicated with described computing machine 1 and LED excitation source 3 respectively simultaneously.
The present embodiment is by using the information of the never synchronous modulation image extracting data of phase shift algorithm focussing plane, obtain the view data of Structured Illumination micro-imaging, and above-mentioned structural light stripes can be via the bright dark control to chip, the rotation of implementation structure striations direction and the movement of structural light stripes phase place.
In the present embodiment, described LED excitation source 3 and intermediate image plane 6, intermediate image plane 6 and illumination on target 9, illumination on target 9 and photodetector 12 imaging surfaces are all in conjugated structure position.
Fig. 3 is the structural representation of LED excitation source described in the utility model.
As shown in Figure 3: described LED excitation source 3 comprises LED substrate 14, many group LED chips 13, positive source 15 and power cathode 16, described many groups LED chip 13, positive source 15 and power cathode 16 are provided with on LED substrate 14, and described many groups LED chip 13 is vertically set up in parallel by group, as, the many groups LED chip 13 described in the present embodiment is provided with eight groups, and label is L1 respectively, L2, L3, L4, L5, L6, L7 and L8, and a wherein electrode tip of described many groups LED chip 13 is all communicated with the electrical connection of the formation common anode utmost point with positive source 15, the another one electrode tip of described multiple LED chips 13 is independently drawn by being communicated with computing machine 1 with the power cathode 16 of the many groups of LED chip 13 equivalent by light path layer, be specially: described power cathode 16 has also been arranged side by side many groups, in the present embodiment, with many groups of LED chip 13 equivalent, be eight groups, label is n1 respectively, n2, n3, n4, n5, n6, n7 and n8, described 8 groups of power cathodes 16 8 groups of LED chips 13 of corresponding control respectively.
Certainly, a wherein electrode tip of described many groups LED chip 13 also can all be communicated with the electrical connection of formation common cathode with power cathode, now, the another one electrode tip of described multiple LED chips 13 is independently drawn by being communicated with computing machine 1 with the positive source 15 of the many groups of LED chip 13 equivalent by light path layer, can independently control to realize every group of LED chip 13.
In addition, in the present embodiment, described LED substrate is metal substrate, and it can be also ceramic substrate certainly, and the luminous wave band of described many groups LED chip 13 is identical or different.When optical system described in the utility model work, control the bright dark of adjacent LED chip 13, thereby form multiple striated structure by computing machine 1, because the bright dark switch speed of LED chip 3 is ns magnitude, high speed that therefore can implementation structure striations is switched.
Fig. 4 is the planar structure schematic diagram of fly's-eye lens described in the utility model unit; Fig. 5 is the side structure schematic diagram of fly's-eye lens described in the utility model unit.
As shown in Figure 3 and Figure 4: described fly's-eye lens unit 41 is arranged in the mode of matrix.And it can be one side fly's-eye lens unit or two-face fly-eye lens unit.In the present embodiment, it is one side fly's-eye lens, and the wherein one side of one side fly's-eye lens unit be plane, and another side is free form surface, and free form surface can effectively reduce the impact of aberration, realizes better even light effect, certain, and it can be also sphere.When system works, each fly's-eye lens unit 41 all carries out imaging to LED chip 13 arrays, thereby in intermediate image plane 6, form the structural light stripes of number multiplication, the multiplication number of the structural light stripes forming in described intermediate image plane 6, is determined by the number of fly's-eye lens unit 41.
Fig. 6 is the structural light stripes schematic diagram that intermediate image plane described in the utility model forms.
As shown in Figure 6, each fly's-eye lens unit 41 in fly eye lens array 4, LED chip 13 arrays are carried out to imaging, thereby the structure number of the structural light stripes that multi-chip structure is formed multiplication, obtain the structural light stripes of nearly continuity, shown in the present embodiment for being close to the striped of sinusoidal structured, the structure of visible each LED chip still in intermediate image plane 6.Certainly, also can, by increasing the number of power electrode, the bright of LED chip 13 secretly be regulated, obtain the structural light stripes of other shapes, all can as circular, square etc.
Fig. 7 is the structural light stripes schematic diagram of illumination on target described in the utility model.
As shown in Figure 7: it is the sinusoidal structured striations forming in illumination on target 9 through projection objective 8, projection objective 8 dwindles striped to image in illumination on target 9, due to the effect of diffraction, in illumination on target 9, form the sinusoidal structured striations of near ideal, certainly, also can secretly regulate the bright of LED chip, obtain the structural light stripes of other shapes, as circular, square etc.
The utility model is by the bright dark variation of LED chip 13, and the high speed of carrying out implementation structure striations is switched and phase shift, and the ns level switching capability of LED chip, by the high speed imaging ability of implementation structure optical illumination microtechnic; And owing to adopting fly eye lens array to replace traditional grating and spatial light modulator, greatly reduce system bulk and cost.
The utility model is illustrated and should be appreciated that above-described embodiment does not limit the utility model in any form according to above-described embodiment, and all employings are equal to replaces or the technical scheme that obtains of equivalent transformation mode, within all dropping on protection domain of the present utility model.
Claims (10)
1. a Structured Illumination optical system, is characterized in that, comprising:
There is the LED excitation source of multiple chip structures;
Be communicated with LED excitation source and independent control the bright dark of each chip and then control whole LED excitation source the computing machine that forms bright dark fringe light;
Be positioned at the relay lens group in the transmitting light path of bright dark fringe light;
Comprise multiple fly's-eye lenses unit, and each fly's-eye lens unit all receives through the bright dark fringe light of relay lens group and carries out imaging with the multiple chip structures to LED excitation source, thereby in intermediate image plane, form the fly eye lens array of the structural light stripes of number multiplication;
For the intermediate image plane of reflection configuration striations;
Be positioned at the dichroic mirror for transmittance structure striations in structural light stripes transmitting light path;
Receive the projection objective that sees through the structural light stripes of dichroic mirror and it is amplified to rear projection according to certain multiplying power;
Be arranged on objective table, after the structural light stripes of reception projection objective projection, form Structured Illumination and excite the illumination on target that produces fluorescence;
Be positioned in the light path of described fluorescence after dichroic mirror reflects, for the cylinder mirror of fluorescence described in transmission;
For the narrow band pass filter that the fluorescence that sees through cylinder mirror is filtered;
For receiving the photodetector of described fluorescence.
2. a kind of Structured Illumination optical system according to claim 1, is characterized in that, also comprises a synchronous control system, and described synchronous control system is communicated with described computing machine and LED excitation source respectively.
3. a kind of Structured Illumination optical system according to claim 1 and 2, is characterized in that, described LED excitation source and intermediate image plane, intermediate image plane and illumination on target, illumination on target and photodetector imaging surface are all in conjugated structure position.
4. a kind of Structured Illumination optical system according to claim 1, it is characterized in that, described LED excitation source comprises LED substrate, many group LED chips, positive source and power cathode, described many groups LED chip, positive source and power cathode are provided with on LED substrate, described many groups LED chip is vertically set up in parallel by group, and a wherein electrode tip of described many groups LED chip is all communicated with the electrical connection of the formation common anode utmost point with positive source, and the another one electrode tip of described multiple LED chips is independently drawn by being communicated with computing machine with LED chip equivalent multi-group power negative pole by light path layer.
5. a kind of Structured Illumination optical system according to claim 1, it is characterized in that, described LED excitation source comprises LED substrate, many group LED chips, positive source and power cathode, described many groups LED chip, positive source and power cathode are provided with on LED substrate, described many groups LED chip is vertically set up in parallel by group, and a wherein electrode tip of described many groups LED chip is all communicated with the electrical connection of formation common cathode with power cathode, and the another one electrode tip of described multiple LED chips is independently drawn by being communicated with computing machine with LED chip equivalent multi-group power is anodal by light path layer.
6. according to a kind of Structured Illumination optical system described in claim 4 or 5, it is characterized in that, described LED substrate is metal substrate or ceramic substrate.
7. a kind of Structured Illumination optical system according to claim 5, is characterized in that, the luminous wave band of described many groups LED chip is identical or different.
8. a kind of Structured Illumination optical system according to claim 1, is characterized in that, described multiple fly's-eye lenses unit is arranged in the mode of matrix.
9. a kind of Structured Illumination optical system according to claim 8, is characterized in that, described fly's-eye lens unit is one side fly's-eye lens unit or two-face fly-eye lens unit.
10. a kind of Structured Illumination optical system according to claim 9, is characterized in that, the wherein one side of described one side fly's-eye lens unit is plane, and another side is free form surface or sphere.
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| CN201420049633.7U CN203732796U (en) | 2014-01-26 | 2014-01-26 | Structured light illumination optical system |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103792654A (en) * | 2014-01-26 | 2014-05-14 | 中国科学院苏州生物医学工程技术研究所 | Structured light lighting optical system |
| CN104570311A (en) * | 2014-12-26 | 2015-04-29 | 中国科学院苏州生物医学工程技术研究所 | Fluorescence microscope based on multi-spectral LED light source |
| CN106019307A (en) * | 2016-05-18 | 2016-10-12 | 北京航空航天大学 | Single-pixel imaging system and method based on array light source |
| CN106707485A (en) * | 2016-12-21 | 2017-05-24 | 中国科学院苏州生物医学工程技术研究所 | Miniature structure light microscopic illumination system |
| CN108169887A (en) * | 2017-12-27 | 2018-06-15 | 清华大学 | The quick micro imaging system of the multifocal area-structure lights of 3D and method |
| CN110082358A (en) * | 2019-05-05 | 2019-08-02 | 苏州天准科技股份有限公司 | A kind of implementation method of Structured Illumination |
-
2014
- 2014-01-26 CN CN201420049633.7U patent/CN203732796U/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103792654A (en) * | 2014-01-26 | 2014-05-14 | 中国科学院苏州生物医学工程技术研究所 | Structured light lighting optical system |
| CN103792654B (en) * | 2014-01-26 | 2015-10-14 | 中国科学院苏州生物医学工程技术研究所 | A kind of Structured Illumination optical system |
| CN104570311A (en) * | 2014-12-26 | 2015-04-29 | 中国科学院苏州生物医学工程技术研究所 | Fluorescence microscope based on multi-spectral LED light source |
| CN106019307A (en) * | 2016-05-18 | 2016-10-12 | 北京航空航天大学 | Single-pixel imaging system and method based on array light source |
| CN106707485A (en) * | 2016-12-21 | 2017-05-24 | 中国科学院苏州生物医学工程技术研究所 | Miniature structure light microscopic illumination system |
| CN106707485B (en) * | 2016-12-21 | 2024-05-03 | 中国科学院苏州生物医学工程技术研究所 | Small-size structure light microscopic lighting system |
| CN108169887A (en) * | 2017-12-27 | 2018-06-15 | 清华大学 | The quick micro imaging system of the multifocal area-structure lights of 3D and method |
| CN110082358A (en) * | 2019-05-05 | 2019-08-02 | 苏州天准科技股份有限公司 | A kind of implementation method of Structured Illumination |
| CN110082358B (en) * | 2019-05-05 | 2022-01-04 | 苏州天准科技股份有限公司 | Method for realizing structured light illumination |
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