CN206479250U - Multiband spectrum imager - Google Patents

Multiband spectrum imager Download PDF

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Publication number
CN206479250U
CN206479250U CN201720077930.6U CN201720077930U CN206479250U CN 206479250 U CN206479250 U CN 206479250U CN 201720077930 U CN201720077930 U CN 201720077930U CN 206479250 U CN206479250 U CN 206479250U
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China
Prior art keywords
light source
collimation
light
spectrum imager
light path
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CN201720077930.6U
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Chinese (zh)
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曾立波
廖乘胜
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WUHAN HER MEDICAL TECHNOLOGY DEVELOPMENT Co Ltd
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WUHAN HER MEDICAL TECHNOLOGY DEVELOPMENT Co Ltd
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  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The utility model provides a kind of multiband spectrum imager, combined light source, collimation assembles component, illuminate slide, microlens, imaging lens and digital imaging camera machine, it is identical that wherein described combined light source includes exit direction, but at least two different monochrome light emission chips of wavelength, on the emitting light path of combined light source component is assembled provided with collimation, component is assembled by collimation to collimate the light that combined light source is sent, assemble, assembled in collimation and the illumination slide is provided with the emitting light path of component, emitting light path through over-illumination slide is provided with the microlens, the emitting light path of microlens is provided with the imaging lens, the emitting light path of imaging lens is provided with digital imagery video camera.The multiband spectrum imager that the utility model is provided, light path is simple, is not required to set filtering device and light-splitting device, passes through the different monochrome light emission chips of switching combining light source, you can realize that medical domain fast multispectral is imaged.

Description

Multiband spectrum imager
Technical field
The utility model is related to medical science spectral imaging technology field, specifically a kind of multiband spectrum imager, uses In the quantitative analysis of the positioning and quantitative analysis, the particularly intracellular members of medical domain of cell component.
Background technology
Spectral imaging technology originates from the multispectral romote sensing technology of 1970s, and the need applied with remote sensing of the earth The most important characteristics and mark wanted and develop spectral imaging technologies are that spectrum and image are combined as a whole, the earth acquired in it Surface image contains abundant space, radiation and the triple information of spectrum, thus in geology, agricultural, vegetation, environment, city, army There is good application in terms of thing, the hydrology, air.
Spectral imaging technology is applied to the detection of biological organization sample, it is qualitative, fixed that biological tissue's spectroscopic data is carried out Amount and positioning analysis, can really realize to biological tissue carry out " where place, have what, how many " comprehensive analysis target, So as to realize the early diagnosis to some pathological changes.
The method that light spectrum image-forming is realized mainly has three kinds at present:
1. using the automatically controlled modulation device filters of the AOTF based on electroacoustic light principle or the principle of interference based on polarised light LCTF Light, the image after filtering is gathered with CCD.The short slab of this kind of method is the inhomogeneities of automatically controlled light filter part, veiling glare and low Transmitance influence is larger, and cost of implementation is higher.
2. the automatically controlled filter wheel being made of thin-film interference filterses is filtered, different optical filters are turned to, are gathered with CCD Image after different wave length optical filtering.Have the disadvantage to be restricted by filter wheel switch speed, image taking speed is slower.
3. the principle of pull-broom type imaging spectrometer in air remote sensing is continued to use, using prism or grating combination element in rear optics System carries out spectrum, and push-scanning image is carried out using the driving of high-precision objective table automatics.Advantage be spectral resolution compared with Height, has the disadvantage that image taking speed is still very slow.
Utility model content
The utility model provides a kind of multiband spectrum imager, is not required to set filtering device and light-splitting device, you can Solve the problem of medical domain fast multispectral is imaged.
To reach above-mentioned purpose, the utility model is implemented by the following technical programs:
Multiband spectrum imager, combined light source, collimation assemble component, illumination slide, microlens, imaging lens sum Word imaging camera machine, wherein the combined light source includes that exit direction is identical but at least two monochromatic light that wavelength is different hair Optical chip, component is assembled on the emitting light path of the combined light source provided with the collimation, and component is assembled to group by the collimation The light that closing light source is sent is collimated, assembled, and is assembled in the collimation on the emitting light path of component provided with the illumination load glass Piece, is provided with the microlens, the emitting light path of the microlens by the emitting light path of the illumination slide and sets There are the imaging lens, the emitting light path of the imaging lens is provided with the digital imagery video camera.
The combined light source includes four monochrome light emission chips.
The monochrome light emission chip of the combined light source is arranged in array.
The emission wavelength of the monochrome light emission chip is any one in 470nm, 530nm, 570nm, 630nm.
The emission wavelength of the monochrome light emission chip can also be other wavelength.
The collimation assembles component, including the collimating mirror and convergent mirror set along light path, and the collimating mirror is to described group Directional light is formed after the light collimation that closing light source is sent, the convergent mirror enters line convergence to the directional light.
The collimation assembles component, in addition to reflecting optics, and the reflecting optics are arranged on the emergent light of the collimating mirror Lu Shang, the convergent mirror is located on the emitting light path of the reflecting optics.
The digital imagery video camera is B/W camera.
Multiband spectrum imager, in addition to:
Camera control circuit, is connected with the digital imagery video camera, for receiving the digital imagery video camera Imaging data;
Memory, is connected with the camera control circuit, receives the imaging data of the camera control circuit, goes forward side by side Row storage.
Multiband spectrum imager, in addition to:
Control circuit for light source, for being connected with the camera control circuit, and according to the camera control circuit Imaging data controls the combined light source to switch different monochrome light emission chip light emittings.
The multiband spectrum imager that the utility model is provided, light path is simple, is not required to set filtering device and light-splitting device, Pass through the different monochrome light emission chips of switching combining light source, you can realize that medical domain fast multispectral is imaged.
Brief description of the drawings
The utility model is described in further detail below according to drawings and examples.
Fig. 1 is a kind of structural representation of multiband spectrum imaging source described in an embodiment.
Fig. 2 is LED (or semiconductor laser) luminescence chip layout structure schematic diagram of combined light source described in an embodiment.
Fig. 3 is a kind of time diagram of multiband light spectrum imaging method described in an embodiment.
Fig. 4 is the circuit diagram of camera control circuit described in an embodiment.
Fig. 5 is the circuit diagram of control circuit for light source described in an embodiment.
Embodiment
As Figure 1-3, the utility model embodiment provides multiband spectrum imager, and combined light source 11, collimation are assembled Component, illumination slide 60, microlens 51, imaging lens 61 and digital imaging camera machine 71, wherein the combined light source 11 is wrapped Include that exit direction is identical but at least two monochrome light emission chips that wavelength is different, in the emitting light path of the combined light source Component is assembled provided with the collimation, the light that combined light source is sent is collimated by the collimation convergence component, assembled, The collimation assembles the emitting light path of component provided with the illumination slide 60, and described illuminate places sample on slide 60, It is provided with by the emitting light path of the illumination slide 60 on the microlens 51, the emitting light path of the microlens 51 Provided with the imaging lens 61, the emitting light path of the imaging lens 61 is provided with the digital imagery video camera 71.
As shown in Fig. 2 the combined light source 11 includes four monochrome light emission chips.Needed depending on application, the monochromatic light Luminescence chip can be 2-16.
The monochrome light emission chip of the combined light source 11 is arranged in array.
The emission wavelength of the monochrome light emission chip is any one in 470nm, 530nm, 570nm, 630nm.
The emission wavelength of the monochrome light emission chip can also be other wavelength.
The collimation assembles component, including the collimating mirror 31 and convergent mirror 41 set along light path, 31 pairs of the collimating mirror Directional light is formed after the light collimation that the combined light source is sent, described 41 pairs of directional lights of convergent mirror enter line convergence.
The collimation assembles component, in addition to reflecting optics 50, and the reflecting optics 50 are arranged on the collimating mirror 31 On emitting light path, the convergent mirror 41 is located on the emitting light path of the reflecting optics 50.
The digital imagery video camera 71 is B/W camera.
As shown in figure 4, the multiband spectrum imager, in addition to:
Camera control circuit 81, is connected with the digital imagery video camera 71, for receiving the digital imagery shooting The imaging data of machine 71;The camera control circuit 81 is using integrated chip POIC.
Memory 21, is connected with the camera control circuit 81, receives the imaging number of the camera control circuit 81 According to, and stored.
As shown in figure 5, the multiband spectrum imager, in addition to:
Control circuit for light source 91, for being connected with the camera control circuit 81, and according to camera control electricity The imaging data on road 81 controls the combined light source 11 to switch different monochrome light emission chip light emittings.
Camera control circuit 81 is signaled to control circuit for light source 91 before camera exposure, lights in combined light source 11 A monochrome light emission chip, be signaled to control circuit for light source after camera exposure terminates, extinguish monochrome light emission core Piece, then according to the wavelength for the monochrome light emission chip lighted, corresponding multi-band image in computer 21 is stored in by digital picture Corresponding wave band;Then light source control module 91 is signaled to before next camera exposure again, is lighted in combined light source Another monochrome light emission chip ..., is circulated successively.
In accompanying drawing 3, the first row represents the sequential of camera exposure, and high level represents camera in exposure.The second to four rows are represented The sequential that each LED (or semiconductor laser) is lighted, it is luminous that high level represents that LED is lit.
Illustrate the process of multispectral imaging by taking four LED (four wave bands) as an example.
LED1 is lit before camera exposure, and is persistently illuminated to after the completion of camera exposure and is just extinguished, now camera exposure The image of collection is a spectrum picture of the wavelength of monochromatic LED 1, is immediately stored.
Before second of exposure of camera, LED2 is lit before camera exposure, and is persistently illuminated to after the completion of camera exposure Just extinguish, the image of now camera exposure collection is a spectrum picture of the wavelength of monochromatic LED 2, is immediately stored.
Before camera third time exposes, LED3 is lit before camera exposure, and is persistently illuminated to after the completion of camera exposure Just extinguish, the image of now camera exposure collection is a spectrum picture of the wavelength of monochromatic LED 4, is immediately stored.
Before the 4th exposure of camera, LED4 is lit before camera exposure, and is persistently illuminated to after the completion of camera exposure Just extinguish, the image of now camera exposure collection is a spectrum picture of the wavelength of monochromatic LED 4, is immediately stored.
The spectrum picture of four storages together form the four band spectrums imaging of observed sample.
In the present embodiment, combined light source 11 is employed such as blue (470nm), green (530nm), red (630nm) and 570nm tetra- color LED integrated LED, this first three color and the photosensitive correspondence of human eye cone cell, are easy to implement colour imaging, while being the discrete list in arrowband Color spectrum, meets Lambert law, is conducive to quantitative analysis, while there is off-the-shelf in this LED market.4th color 570nm monochrome LED, for cell DNA quantitative measurment, the dyestuff of cell feulgen's stain absorbs very strong on this wavelength.
Using the utility model, 470nm, 530nm, 570nm and 630nm micro- multispectral fast short-term training of four wave bands are realized Picture;By related analytical technology, realize Pasteur and Fu Ergen redye under the conditions of cell DNA ploidy analysis, meet same The demand of the analysis of DNA ploidy body and TBS cell analysis is realized on Shi Yi slides.
The multiband spectrum imager that the utility model is provided, light path is simple, is not required to set filtering device and light-splitting device, Pass through the different monochrome light emission chips of switching combining light source, you can realize that medical domain fast multispectral is imaged.
Finally it should be noted that:The preferred embodiment of utility model is the foregoing is only, practicality is not limited to It is new, although utility model is described in detail with reference to the foregoing embodiments, for those skilled in the art, its The technical scheme described in foregoing embodiments can still be modified, or which part technical characteristic is equal Replace.All spirit in utility model is with principle, and any modification, equivalent substitution and improvements made etc. should be included in reality Within new protection domain.

Claims (9)

1. multiband spectrum imager, combined light source, collimation assemble component, illumination slide, microlens, imaging lens and numeral Imaging camera machine, it is characterised in that wherein described combined light source includes that exit direction is identical but wavelength is different at least two Monochrome light emission chip, component is assembled on the emitting light path of the combined light source provided with the collimation, is assembled by the collimation Component is collimated to the light that combined light source is sent, assembled, and is assembled in the collimation on the emitting light path of component provided with described Slide is illuminated, the microlens, the outgoing of the microlens are provided with by the emitting light path of the illumination slide Light path is provided with the imaging lens, and the emitting light path of the imaging lens is provided with the digital imagery video camera.
2. multiband spectrum imager as claimed in claim 1, it is characterised in that
The combined light source includes four monochrome light emission chips.
3. multiband spectrum imager as claimed in claim 1 or 2, it is characterised in that
The monochrome light emission chip of the combined light source is arranged in array.
4. multiband spectrum imager as claimed in claim 3, it is characterised in that
The emission wavelength of the monochrome light emission chip is any one in 470nm, 530nm, 570nm, 630nm.
5. multiband spectrum imager as claimed in claim 1, it is characterised in that
The collimation assembles component, including the collimating mirror and convergent mirror set along light path, and the collimating mirror is to the combination light Directional light is formed after the light collimation that source is sent, the convergent mirror enters line convergence to the directional light.
6. multiband spectrum imager as claimed in claim 5, it is characterised in that
The collimation assembles component, in addition to reflecting optics, and the reflecting optics are arranged on the emitting light path of the collimating mirror, The convergent mirror is located on the emitting light path of the reflecting optics.
7. multiband spectrum imager as claimed in claim 1, it is characterised in that
The digital imagery video camera is B/W camera.
8. multiband spectrum imager as claimed in claim 1, it is characterised in that
Multiband spectrum imager, in addition to:
Camera control circuit, is connected with the digital imagery video camera, the imaging for receiving the digital imagery video camera Data;
Memory, is connected with the camera control circuit, receives the imaging data of the camera control circuit, and is deposited Storage.
9. multiband spectrum imager as claimed in claim 8, it is characterised in that
Multiband spectrum imager, in addition to:
Control circuit for light source, for being connected with the camera control circuit, and according to the imaging of the camera control circuit Data control the combined light source to switch different monochrome light emission chip light emittings.
CN201720077930.6U 2017-01-20 2017-01-20 Multiband spectrum imager Active CN206479250U (en)

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CN201720077930.6U CN206479250U (en) 2017-01-20 2017-01-20 Multiband spectrum imager

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Application Number Priority Date Filing Date Title
CN201720077930.6U CN206479250U (en) 2017-01-20 2017-01-20 Multiband spectrum imager

Publications (1)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110293536A (en) * 2019-07-12 2019-10-01 哈尔滨工业大学 A kind of micro-nano robot control system

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110293536A (en) * 2019-07-12 2019-10-01 哈尔滨工业大学 A kind of micro-nano robot control system
CN110293536B (en) * 2019-07-12 2020-09-18 哈尔滨工业大学 Micro-nano robot control system

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