CN107033209A - It is a kind of at the same extract the method without the residual mogroside of agriculture and water-soluble dietary fiber - Google Patents
It is a kind of at the same extract the method without the residual mogroside of agriculture and water-soluble dietary fiber Download PDFInfo
- Publication number
- CN107033209A CN107033209A CN201710388435.1A CN201710388435A CN107033209A CN 107033209 A CN107033209 A CN 107033209A CN 201710388435 A CN201710388435 A CN 201710388435A CN 107033209 A CN107033209 A CN 107033209A
- Authority
- CN
- China
- Prior art keywords
- water
- agriculture
- dietary fiber
- soluble dietary
- residual
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 45
- 229930189775 mogroside Natural products 0.000 title claims abstract description 44
- 239000000284 extract Substances 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 39
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 70
- 241001409321 Siraitia grosvenorii Species 0.000 claims abstract description 48
- 235000011171 Thladiantha grosvenorii Nutrition 0.000 claims abstract description 48
- 238000001728 nano-filtration Methods 0.000 claims abstract description 38
- 239000012466 permeate Substances 0.000 claims abstract description 25
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 24
- 239000012465 retentate Substances 0.000 claims abstract description 24
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 23
- 238000004587 chromatography analysis Methods 0.000 claims abstract description 20
- 239000012530 fluid Substances 0.000 claims abstract description 15
- 238000005119 centrifugation Methods 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 11
- 239000012528 membrane Substances 0.000 claims abstract description 11
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000010828 elution Methods 0.000 claims abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims description 21
- 238000001035 drying Methods 0.000 claims description 17
- 235000021022 fresh fruits Nutrition 0.000 claims description 15
- 238000000605 extraction Methods 0.000 claims description 9
- 239000007787 solid Substances 0.000 claims description 9
- 239000002023 wood Substances 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 5
- 235000015203 fruit juice Nutrition 0.000 claims description 5
- 229910052799 carbon Inorganic materials 0.000 claims description 4
- 239000003245 coal Substances 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 4
- 239000003610 charcoal Substances 0.000 claims 1
- 229930182470 glycoside Natural products 0.000 abstract description 19
- 150000002338 glycosides Chemical class 0.000 abstract description 19
- 238000004519 manufacturing process Methods 0.000 abstract description 12
- 239000000047 product Substances 0.000 abstract description 5
- 239000003905 agrochemical Substances 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 12
- 239000012535 impurity Substances 0.000 description 11
- 235000009508 confectionery Nutrition 0.000 description 8
- 239000000575 pesticide Substances 0.000 description 8
- 244000269722 Thea sinensis Species 0.000 description 7
- 229940023032 activated charcoal Drugs 0.000 description 7
- 239000003513 alkali Substances 0.000 description 7
- 235000009815 Momordica Nutrition 0.000 description 6
- 241000218984 Momordica Species 0.000 description 6
- 235000013399 edible fruits Nutrition 0.000 description 6
- 239000012153 distilled water Substances 0.000 description 5
- 239000002994 raw material Substances 0.000 description 5
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 4
- DLRVVLDZNNYCBX-UHFFFAOYSA-N Polydextrose Polymers OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(O)O1 DLRVVLDZNNYCBX-UHFFFAOYSA-N 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- 239000006013 carbendazim Substances 0.000 description 4
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 4
- 230000002255 enzymatic effect Effects 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- GHBNZZJYBXQAHG-KUVSNLSMSA-N (2r,3r,4s,5s,6r)-2-[[(2r,3s,4s,5r,6r)-6-[[(3s,8s,9r,10r,11r,13r,14s,17r)-17-[(2r,5r)-5-[(2s,3r,4s,5s,6r)-4,5-dihydroxy-3-[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-6-[[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@H](CC[C@@H](C)[C@@H]1[C@]2(C[C@@H](O)[C@@]3(C)[C@H]4C(C([C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO[C@H]6[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O6)O)O5)O)CC4)(C)C)=CC[C@H]3[C@]2(C)CC1)C)C(C)(C)O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O GHBNZZJYBXQAHG-KUVSNLSMSA-N 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000004811 liquid chromatography Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- TVJXHJAWHUMLLG-UHFFFAOYSA-N mogroside V Natural products CC(CCC(OC1OC(COC2OC(CO)C(O)C(O)C2OC3OC(CO)C(O)C(O)C3O)C(O)C(O)C1O)C(C)(C)O)C4CCC5(C)C6CC=C7C(CCC(OC8OC(COC9OC(CO)C(O)C(O)C9O)C(O)C(O)C8O)C7(C)C)C6(C)C(O)CC45C TVJXHJAWHUMLLG-UHFFFAOYSA-N 0.000 description 3
- 230000007935 neutral effect Effects 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
- 229920001100 Polydextrose Polymers 0.000 description 2
- 238000010306 acid treatment Methods 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 239000004411 aluminium Substances 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000008101 lactose Substances 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000001814 pectin Substances 0.000 description 2
- 229920001277 pectin Polymers 0.000 description 2
- 235000010987 pectin Nutrition 0.000 description 2
- 239000001259 polydextrose Substances 0.000 description 2
- 229940035035 polydextrose Drugs 0.000 description 2
- 235000013856 polydextrose Nutrition 0.000 description 2
- 150000008442 polyphenolic compounds Chemical class 0.000 description 2
- 235000013824 polyphenols Nutrition 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- ZMYFCFLJBGAQRS-IRXDYDNUSA-N (2R,3S)-epoxiconazole Chemical compound C1=CC(F)=CC=C1[C@@]1(CN2N=CN=C2)[C@H](C=2C(=CC=CC=2)Cl)O1 ZMYFCFLJBGAQRS-IRXDYDNUSA-N 0.000 description 1
- QNBTYORWCCMPQP-JXAWBTAJSA-N (Z)-dimethomorph Chemical compound C1=C(OC)C(OC)=CC=C1C(\C=1C=CC(Cl)=CC=1)=C/C(=O)N1CCOCC1 QNBTYORWCCMPQP-JXAWBTAJSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 239000005761 Dimethomorph Substances 0.000 description 1
- 239000005767 Epoxiconazole Substances 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 240000006053 Garcinia mangostana Species 0.000 description 1
- 235000017048 Garcinia mangostana Nutrition 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 150000003851 azoles Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000010812 external standard method Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 235000021096 natural sweeteners Nutrition 0.000 description 1
- 150000002825 nitriles Chemical class 0.000 description 1
- UWVQIROCRJWDKL-UHFFFAOYSA-N oxadixyl Chemical compound CC=1C=CC=C(C)C=1N(C(=O)COC)N1CCOC1=O UWVQIROCRJWDKL-UHFFFAOYSA-N 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 229940126680 traditional chinese medicines Drugs 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J17/00—Normal steroids containing carbon, hydrogen, halogen or oxygen, having an oxygen-containing hetero ring not condensed with the cyclopenta(a)hydrophenanthrene skeleton
- C07J17/005—Glycosides
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Sustainable Development (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
It is a kind of at the same extract the method without the residual mogroside of agriculture and water-soluble dietary fiber, comprise the following steps：（1）Momordica grosvenori is cleaned, squeezed the juice, pomace elution obtains extract solution；（2）Boil, cool down, centrifugation obtains centrifugate；（3）By the aluminum oxide and activated carbon chromatography post of tandem, efflux is collected；（4）Ultrafiltration membrance filter, collects trapped fluid and permeate respectively；（5）Nanofiltration, concentration：By ultrafiltration permeate nanofiltration membrane, trapped fluid and permeate are collected respectively, and nanofiltration retentate fluid concentration is dried, obtains the residual mogroside of no agriculture；Nanofiltration permeate liquid is merged into concentration with ultra-filter retentate, dries, obtains the residual Momordica grosvenori water-soluble dietary fiber of no agriculture.Gained mogroside and the equal non agricultural chemical residuum of water-soluble dietary fiber product；Glycosides V content is up to 90.6%, and yield is up to 96.3%；The purity of water-soluble dietary fiber is up to 95%, and yield is up to 91%；The inventive method is simple, easy to operate, is adapted to industrialization production.
Description
Technical field
The present invention relates to the production method of Fructus Monordicae extract, and in particular to one kind extracts nothing simultaneously from Momordica grosvenori fresh fruit
The method of the residual mogroside of agriculture and water-soluble dietary fiber.
Background technology
Momordica grosvenori sugariness is high, and heat is low, with the health care such as raising immunity, protection liver, hypoglycemic, anticancer, anti-oxidant
Function, therefore, health ministry, the State Administration of Traditional Chinese Medicines are included in first " being both food and the kind list of medicine ",
Meanwhile, and originated as preferable natural sweetener.
At present, China's Fructus Monordicae extract industry development has begun to take shape, and is mainly to extract and separate sweet Momordica grosvenori
Based on glycosides.However, the beneficiating ingredient in Momordica grosvenori is in addition to mogroside, also other important components, such as dietary fiber.
The simple sweet tea glycosides using in Momordica grosvenori, and other compositions are treated as discarded object or wastewater treatment, it is current Fructus Monordicae extract row
The customary practice of industry, not only causes serious pollution to environment, adds the cost of waste water control, while being also to Momordica grosvenori
The huge waste of resource.Further, since indiscriminate use of pesticide, the reason, city such as coarse of the backwardness of production technology and process equipment
Most of Fructus Monordicae extract on face is difficult to reach that low agriculture is residual, the even residual quality requirement without agriculture.
CN105273033A disclose it is a kind of from Momordica grosvenori extract mogroside method, be using Momordica grosvenori dry fruit as
Raw material, by crushing, microwave radiation exaraction, ultrafiltration, nanofiltration, concentration, drying and other steps, obtains mogroside.But, due to
This method is not handled targetedly the residues of pesticides in raw material, and product quality is unable to reach the residual organic standard of no agriculture, and
Other compositions that this method can only obtain in mogroside, Momordica grosvenori are not used.
CN102742801A discloses Momordica grosvenori high dietary-fiber fruit powder and preparation method thereof, is by by mangosteen powder
The steps such as broken, extraction, concentration, alcohol precipitation, alkali process, acid treatment, obtain Siraitia grosvenorii high dietary cellulose fruit powder.But, due to this
Method largely uses organic solvent and soda acid, and pollution is big, and cost is high, and does not isolate in Momordica grosvenori most sieve of economic value
Chinese fruit sweet tea glycosides, it is impossible to meet the requirement of industrialized production.
CN105124520A discloses the method for removing pesticide residue from Fructus Monordicae extract, is by sieve of powdery
Chinese berry extract is dissolved in after water, passes through the post of granular activated carbon.But, this method removes residues of pesticides and activated carbon one kind is used only
Approach, therefore, the effect for removing the residual species of agriculture and removal are all limited.In addition, this method is not related to sweet tea glycosides and dietary fiber
Separation, and this method needs are using Fructus Monordicae extract finished product as raw material, it is with high costs, it is not suitable for industrialization.
CN104431841A discloses a kind of method for reducing carbendazim in Fructus Monordicae extract, is first to use activated-charcoal column
Alkali and acid treatment, then the Fructus Monordicae extract aqueous solution is flowed through into activated-charcoal column, elute, nanofiltration, be concentrated in vacuo, spray drying is obtained
It is less than 0.01mg/kg to carbendazim content, mogroside V content is higher than 40% extract.But, this method is only to Momordica grosvenori
A kind of residues of pesticides of carbendazim in extract play the role of part removal, and the content of extract mogroside V is relatively low.This
Outside, this method can only obtain the Fructus Monordicae extract containing Momordica grosvenori mogroside V, can not obtain Momordica grosvenori dietary fiber.
The content of the invention
The technical problems to be solved by the invention are that there is provided a kind of product yield, pure for the defect that overcomes prior art to exist
Degree is high, residual without agriculture, and technical process is workable, and cost is low, no pollution, is suitable for the same from Momordica grosvenori of industrialized production
When extract the method without the residual mogroside of agriculture and water-soluble dietary fiber.
The technical solution adopted for the present invention to solve the technical problems is as follows：It is a kind of to extract simultaneously without the residual mogroside of agriculture
With the method for water-soluble dietary fiber, comprise the following steps：
（1）Squeeze the juice extraction：Momordica grosvenori fresh fruit is cleaned, squeezed the juice at room temperature, pomace uses water wash again, merges fruit juice and leacheate,
Obtain extract solution；
（2）Boil：By step（1）Gained extract solution boils, and is cooled to room temperature, centrifugation, obtains centrifugate；
（3）Chromatography：By step（2）Gained centrifugate is collected by the alumina chromatographic column and activated carbon chromatography post of tandem
Efflux；
（4）Ultrafiltration：By step（3）Gained efflux ultrafiltration membrance filter, collects ultra-filter retentate and ultrafiltration permeate respectively；
（5）Nanofiltration, concentration：By step（3）Gained ultrafiltration permeate nanofiltration membrane, collects nanofiltration retentate fluid and nanofiltration respectively
Permeate, nanofiltration retentate fluid is concentrated under reduced pressure, and is dried, is obtained the residual mogroside of no agriculture；By gained nanofiltration permeate liquid and step（4）
Gained ultra-filter retentate merges, and is concentrated under reduced pressure, and dries, obtains the residual Momordica grosvenori water-soluble dietary fiber of no agriculture.
Preferably, step（1）In, in the Momordica grosvenori fresh fruit, the mass content of momordica glycoside V is 0.3~0.8%, water
The mass content of soluble dietary fiber is 5~10%.The water-soluble dietary fiber includes pectin, polydextrose of macromolecular etc.,
And FOS, oligoisomaltose, oligomeric lactose, the xylo-oligosaccharide of small molecule etc..
Preferably, step（1）In, the mode squeezed the juice is squeezed the juice for spiral squeezing type.Equipment used in squeezing the juice is spiral shell
Extrusion juice extractor is revolved, the juice extractor juicing effect is high, and pomace easily elutes and drained；The clean purpose is to remove fresh fruit
The most agriculture in surface is residual；The purpose that room temperature is squeezed the juice is the leaching of impurity when avoiding the high temperature from squeezing the juice.
Preferably, step（1）In, the consumption for the water of elution is 0.8~1.2 times of fresh fruit quality.
Preferably, step（2）In, the time boiled is 10~30min.The purpose boiled is to make the egg in extract solution
White matter is denatured, condensed at high temperature, if the time boiled is too short, and protein has little time to be denatured and can not condense；If boiling
Overlong time, then the water-soluble dietary fiber and mogroside in extract solution, which are easily charred, is even carbonized.
Preferably, step（2）In, the rotating speed of the centrifugation is 8000~30000r/min.The purpose of centrifugation be by from
Solid-liquor separation if the rotating speed of centrifugation is excessively slow, is difficult to the purpose for reaching thorough separation of solid and liquid by mental and physical efforts；If the rotating speed mistake of centrifugation
It hurry up, then easily cause the loss of equipment.
Preferably, step（3）In, the consumption of aluminum oxide is the 0.05 of Momordica grosvenori fresh fruit quality in the alumina chromatographic column
~1.00 times（More preferably 0.08~0.50 times, still more preferably 0.1~0.2 times）.If the consumption of aluminum oxide is very few, impurity
Absorption it is not thorough enough；If the consumption of aluminum oxide is excessive, the waste of material is easily caused.
Preferably, step（3）In, the particle diameter of the aluminum oxide is 100~300 mesh.The type of the aluminum oxide is acidity
Aluminum oxide, neutral alumina or alkali alumina, the purpose of alumina column chromatography are to adsorb organic acid, the flavones in centrifugate
The impurity components such as class, Polyphenols and fat-soluble residues of pesticides.
It is preferred that, step（3）In, the ratio of height to diameter of the alumina chromatographic column is 1~12:1（More preferably 5~10:1）, on
The flow velocity of post is 0.4~5.0BV/h（More preferably 0.5~2.0BV/h）.If alumina chromatographic column ratio of height to diameter is too small or flow velocity mistake
It hurry up, impurity will all be difficult to abundant absorption；If alumina chromatographic column ratio of height to diameter is excessive or flow velocity is too small, it will all extend the cycle of production
And increase the cost of production.
Preferably, step（3）In, the consumption of activated carbon is the 0.05 of Momordica grosvenori fresh fruit quality in the activated carbon chromatography post
~2.00 times（More preferably 0.08~0.50 times, still more preferably 0.1~0.2 times）.If the consumption of activated carbon is very few, impurity
It will adsorb not thorough；If the consumption of activated carbon is excessive, the waste of material is easily caused.
Preferably, step（3）In, the particle diameter of the activated carbon is 10~500 mesh（More preferably 100~400 mesh）.
Preferably, step（3）In, the activated carbon is wood activated charcoal or coal mass active carbon.The purpose of activated carbon chromatography
It is the impurity components such as pigment and water soluble pesticide residual in adsorption and oxidation aluminium efflux.Because activated carbon is residual to fat-soluble agriculture several
Without adsorption capacity, and the absorption residual to water-soluble agriculture is easily lost by the residual pollution of fat-soluble agriculture, therefore, it is necessary to will oxidation
Aluminium chromatographic column is placed in before activated carbon chromatography post.
Preferably, step（3）In, the ratio of height to diameter of the activated carbon chromatography post is 1~10:1（More preferably 4~8:1）, upper prop
Flow velocity be 0.4~5.0BV/h（More preferably 0.5~2.0BV/h）.If activated carbon chromatography post ratio of height to diameter is too small or flow velocity is too fast,
Impurity will all be difficult to abundant absorption；If activated carbon chromatography post ratio of height to diameter is excessive or flow velocity is too small, will all it extend the cycle of production simultaneously
Increase the cost of production.
Preferably, step（4）In, the molecular cut off of the milipore filter is 1~50,000 Da, the pressure of filtering for 0.1~
1.2MPa（More preferably 0.5~1.0MPa）.The purpose of ultrafiltration is to remove the molecular weight material bigger than mogroside, therefore, is surpassed
It is pectin, polydextrose etc. to filter the main component in trapped fluid.If ultrafiltration retaining molecular weight is too small, mogroside is by part
It is retained in ultra-filter retentate, the yield for causing mogroside is relatively low；If ultrafiltration retaining molecular weight is excessive, big point of part
The impurity of son amount will can not be separated with mogroside molecule also cross milipore filter, and subsequent step, cause mogroside
Purity it is relatively low.
Preferably, step（5）In, the molecular cut off of the NF membrane is 1000~5000Da（More preferably 2000~
3000Da）, the pressure of filtering is 0.2~2.0MPa（More preferably 1.0~1.5MPa）.The purpose of nanofiltration is to remove molecular weight to compare sieve
The small material of Chinese fruit sweet tea glycosides, therefore, main component in nanofiltration permeate liquid is FOS, oligoisomaltose, oligomeric lactose,
Xylo-oligosaccharide etc..If nanofiltration retaining molecular weight is too small, the impurity of part small-molecular-weight will be unable to by NF membrane, same to Momordica grosvenori
Sweet tea glycosides is partly retained in nanofiltration retentate fluid together, causes the purity of mogroside relatively low；If nanofiltration retaining molecular weight mistake
Greatly, part mogroside also will be relatively low by the yield for causing mogroside by NF membrane.
Preferably, step（5）In, the vacuum being concentrated under reduced pressure is -0.06~-0.09MPa, and temperature is 65~80
DEG C, it is 20~50% to be concentrated into solid content（More preferably 25~40%）.
Preferably, step（5）In, the drying is microwave drying, and the temperature of microwave drying is 60~80 DEG C, microwave drying
Vacuum be -0.05~-0.09MPa, time of microwave drying is 1~4h.
In the inventive method is distilled water or pure water or step with water（5）Collected by being concentrated under reduced pressure
Distilled water；Due to residual without agriculture in pure water or distilled water, the residual pollution of additional agriculture can be avoided.
The principle of the inventive method is：Momordica grosvenori squeeze the juice extract solution by alumina chromatographic column can remove organic acid,
The impurity such as flavonoids, Polyphenols and fat-soluble residues of pesticides, activated carbon chromatography post can be except depigmentaton and water soluble pesticide residual
Deng impurity, and mogroside therein and water-soluble dietary fiber composition can be divided by the screening of milipore filter and NF membrane
From so as to obtain the residual mogroside of no agriculture and without the residual Momordica grosvenori water-soluble dietary fiber of agriculture.
The inventive method has the beneficial effect that：
（1）After testing, mogroside obtained by the inventive method and the equal non agricultural chemical residuum of Momordica grosvenori water-soluble dietary fiber, reach
European Union's agriculture residual limit standard；Glycosides V content is up to 90.6% in mogroside, and the high income of momordica glycoside V is up to 96.3%；
The product purity of Momordica grosvenori water-soluble dietary fiber is up to 95%, and high income is up to 91%；
（2）The inventive method is compared with existing Momordica grosvenori extracting method, and the inventive method is in extraction and separation process only with a small amount of
Water is as solvent, without using any organic solvent, and by last concentration step, the distilled water of recovery can be with circulating repetition profit
With whole production process sewage zero-discharge has been truly realized clean manufacturing, green, economical, safe and environment-friendly；The inventive method is set
It is standby simple, easy to operate, it is adapted to industrialization production.
Embodiment
With reference to embodiment, the invention will be further described.
Momordica grosvenori fresh fruit used in the embodiment of the present invention is purchased from Guilin, and the mass content of momordica glycoside V is
0.5%, the mass content of water-soluble dietary fiber is 7.2%；The residual mass content of raw material agriculture is 0.20ppm, wherein, dimethomorph
0.05ppm, nitrile bacterium azoles 0.04ppm, epoxiconazole 0.02ppm, Wakil 0.03ppm, carbendazim 0.06ppm；In the inventive method
Be the residual distilled water of no agriculture with water；Raw material used in the embodiment of the present invention or chemical reagent, unless otherwise specified, lead to
Cross the acquisition of routine business approach.
In the embodiment of the present invention, using gas chromatograph-mass spectrometer (GC-MS)（GC-MS）Detect that agriculture is residual, using enzymatic gravimetric method-liquid
Phase chromatography detects the content of Momordica grosvenori water-soluble dietary fiber, using high performance liquid chromatography（HPLC）External standard method detects Momordica grosvenori
Sweet tea glycosides V content.
Embodiment 1
（1）Squeeze the juice extraction：500kg Momordica grosvenori fresh fruits are eluted with water, squeezed the juice at room temperature with spiral squeezing type juice extractor, pomace is again
Water wash is distilled with 500kg, merges fruit juice and leacheate, obtains extract solution 750kg；
（2）Boil：By step（1）Gained extract solution boils 20min, is cooled to room temperature, under the conditions of rotating speed is 10000r/min
Centrifugation, obtains centrifugate；
（3）Chromatography：By step（2）Gained centrifugate is chromatographed by the alkali alumina chromatographic column and wood activated charcoal of tandem
Post, collects efflux；The mesh number of the alkali alumina is 100 mesh, and consumption is 50kg, and the ratio of height to diameter of alumina chromatographic column is 5:
1, the flow velocity of upper prop is 0.5BV/h；The mesh number of the wood activated charcoal is 100 mesh, and consumption is 60kg, the height of activated carbon chromatography post
Footpath ratio is 5:1, the flow velocity of upper prop is 0.5BV/h；
（4）Ultrafiltration：By step（3）Gained efflux molecular cut off is 10,000 Da milipore filter, the mistake in the case where pressure is 0.6MPa
Filter, collects ultra-filter retentate and ultrafiltration permeate respectively；
（5）Nanofiltration, concentration：By step（3）The NF membrane that gained ultrafiltration permeate is 3000Da with molecular cut off, be in pressure
In vacuum it is -0.08MPa, 70 by nanofiltration retentate fluid 1.5MPa lower filtering, collects nanofiltration retentate fluid and nanofiltration permeate liquid respectively
At DEG C, it is 25% to be concentrated under reduced pressure into solid content, and in the case where 68 DEG C, vacuum are -0.09MPa, microwave drying 2h obtains no agriculture residual
Mogroside 2.62kg；By gained nanofiltration permeate liquid and step（4）Gained ultra-filter retentate merge, vacuum for-
0.08MPa, at 70 DEG C, it is 20% to be concentrated under reduced pressure into solid content, in the case where 68 DEG C, vacuum are -0.09MPa, microwave drying
2h, obtains the residual Momordica grosvenori water-soluble dietary fiber 34.1kg of no agriculture.
Detected through GC-MS, 400 remainder agricultures are residual in mogroside obtained by the present embodiment and Momordica grosvenori water-soluble dietary fiber
Testing result meets the residual limit standard of European Union's agriculture；Detected through HPLC, glycosides V content is in mogroside obtained by the present embodiment
90.6%, the yield of momordica glycoside V is 95%；Through enzymatic gravimetric method-liquid chromatography detection, Momordica grosvenori obtained by the present embodiment is water-soluble
Property dietary fiber purity be 95%, the yield of water-soluble dietary fiber is 90%.
Embodiment 2
（1）Squeeze the juice extraction：1500kg Momordica grosvenori fresh fruits are eluted with water, squeezed the juice at room temperature with spiral squeezing type juice extractor, pomace
Water wash is distilled with 1350kg again, merges fruit juice and leacheate, obtains extract solution 2100kg；
（2）Boil：By step（1）Gained extract solution boils 30min, is cooled to room temperature, under the conditions of rotating speed is 20000r/min
Centrifugation, obtains centrifugate；
（3）Chromatography：By step（2）Gained centrifugate is chromatographed by the neutral alumina chromatographic column and wood activated charcoal of tandem
Post, collects efflux；The mesh number of the neutral alumina is 300 mesh, and consumption is 180kg, and the ratio of height to diameter of alumina chromatographic column is
10:1, the flow velocity of upper prop is 2BV/h；The mesh number of the wood activated charcoal is 200 mesh, and consumption is 150kg, activated carbon chromatography post
Ratio of height to diameter is 8:1, the flow velocity of upper prop is 2BV/h；
（4）Ultrafiltration：By step（3）Gained efflux molecular cut off is 30,000 Da milipore filter, the mistake in the case where pressure is 0.5MPa
Filter, collects ultra-filter retentate and ultrafiltration permeate respectively；
（5）Nanofiltration, concentration：By step（3）The NF membrane that gained ultrafiltration permeate is 2000Da with molecular cut off, be in pressure
Filtered under 1MPa, nanofiltration retentate fluid and nanofiltration permeate liquid are collected respectively, in vacuum be -0.08MPa, 75 DEG C by nanofiltration retentate fluid
Under, it is 25% to be concentrated under reduced pressure into solid content, and in the case where 70 DEG C, vacuum are -0.08MPa, microwave drying 1h obtains residual sieve of no agriculture
Chinese fruit sweet tea glycosides 10.3kg；By gained nanofiltration permeate liquid and step（4）Gained ultra-filter retentate merge, vacuum for-
0.08MPa, at 75 DEG C, it is 25% to be concentrated under reduced pressure into solid content, in the case where 70 DEG C, vacuum are -0.08MPa, microwave drying
1h, obtains the residual Momordica grosvenori water-soluble dietary fiber 101.8kg of no agriculture.
Detected through GC-MS, 400 remainder agricultures are residual in mogroside obtained by the present embodiment and Momordica grosvenori water-soluble dietary fiber
Testing result meets the residual limit standard of European Union's agriculture；Detected through HPLC, glycosides V content is in mogroside obtained by the present embodiment
70.1%, the yield of momordica glycoside V is 96.3%；Through enzymatic gravimetric method-liquid chromatography detection, Momordica grosvenori water obtained by the present embodiment
The purity of soluble dietary fiber is 92.3%, and the yield of water-soluble dietary fiber is 87%.
Embodiment 3
（1）Squeeze the juice extraction：1000kg Momordica grosvenori fresh fruits are eluted with water, squeezed the juice at room temperature with spiral squeezing type juice extractor, pomace
Water wash is distilled with 1100kg again, merges fruit juice and leacheate, obtains extract solution 1600kg；
（2）Boil：By step（1）Gained extract solution boils 15min, is cooled to room temperature, under the conditions of rotating speed is 30000r/min
Centrifugation, obtains centrifugate；
（3）Chromatography：By step（2）Gained centrifugate is chromatographed by the alkali alumina chromatographic column and coal mass active carbon of tandem
Post, collects efflux；The mesh number of the alkali alumina is 200 mesh, and consumption is 200kg, and the ratio of height to diameter of alumina chromatographic column is
5:1, the flow velocity of upper prop is 1BV/h；The mesh number of the coal mass active carbon is 400 mesh, and consumption is 150kg, activated carbon chromatography post
Ratio of height to diameter is 6:1, the flow velocity of upper prop is 1BV/h；
（4）Ultrafiltration：By step（3）Gained efflux molecular cut off is 20,000 Da milipore filter, the mistake in the case where pressure is 1.0MPa
Filter, collects ultra-filter retentate and ultrafiltration permeate respectively；
（5）Nanofiltration, concentration：By step（3）The NF membrane that gained ultrafiltration permeate is 2500Da with molecular cut off, be in pressure
Filtered under 1.5MPa, nanofiltration retentate fluid and nanofiltration permeate liquid collected respectively, in vacuum be -0.075MPa by nanofiltration retentate fluid,
At 75 DEG C, it is 35% to be concentrated under reduced pressure into solid content, and in the case where 80 DEG C, vacuum are -0.07MPa, microwave drying 3h obtains no agriculture
Residual mogroside 6.2kg；By gained nanofiltration permeate liquid and step（4）Gained ultra-filter retentate merge, vacuum for-
0.075MPa, at 75 DEG C, it is 35% to be concentrated under reduced pressure into solid content, in the case where 80 DEG C, vacuum are -0.07MPa, microwave drying
3h, obtains the residual Momordica grosvenori water-soluble dietary fiber 71.6kg of no agriculture.
Detected through GC-MS, 400 remainder agricultures are residual in mogroside obtained by the present embodiment and Momordica grosvenori water-soluble dietary fiber
Testing result meets the residual limit standard of European Union's agriculture；Detected through HPLC, glycosides V content is in mogroside obtained by the present embodiment
75.2%, the yield of momordica glycoside V is 93.2%；Through enzymatic gravimetric method-liquid chromatography detection, Momordica grosvenori water obtained by the present embodiment
The purity of soluble dietary fiber is 91.5%, and the yield of water-soluble dietary fiber is 91%.
Claims (9)
1. it is a kind of while the method without the residual mogroside of agriculture and water-soluble dietary fiber of extraction, it is characterised in that including following
Step：
（1）Squeeze the juice extraction：Momordica grosvenori fresh fruit is cleaned, squeezed the juice at room temperature, pomace uses water wash again, merges fruit juice and leacheate,
Obtain extract solution；
（2）Boil：By step（1）Gained extract solution boils, and is cooled to room temperature, centrifugation, obtains centrifugate；
（3）Chromatography：By step（2）Gained centrifugate is collected by the alumina chromatographic column and activated carbon chromatography post of tandem
Efflux；
（4）Ultrafiltration：By step（3）Gained efflux ultrafiltration membrance filter, collects ultra-filter retentate and ultrafiltration permeate respectively；
（5）Nanofiltration, concentration：By step（3）Gained ultrafiltration permeate nanofiltration membrane, collects nanofiltration retentate fluid and nanofiltration respectively
Permeate, nanofiltration retentate fluid is concentrated under reduced pressure, and is dried, is obtained the residual mogroside of no agriculture；By gained nanofiltration permeate liquid and step（4）
Gained ultra-filter retentate merges, and is concentrated under reduced pressure, and dries, obtains the residual Momordica grosvenori water-soluble dietary fiber of no agriculture.
2. extract the method without the residual mogroside of agriculture and water-soluble dietary fiber, its feature simultaneously according to claim 1
It is, step（1）In, the mode squeezed the juice is squeezed the juice for spiral squeezing type.
3. it is according to claim 1 or claim 2 while the method without the residual mogroside of agriculture and water-soluble dietary fiber of extraction, it is special
Levy and be, step（1）In, the consumption for the water of elution is 0.8~1.2 times of fresh fruit quality.
4. extract the method without the residual mogroside of agriculture and water-soluble dietary fiber simultaneously according to one of claims 1 to 3,
Characterized in that, step（2）In, the time boiled is 10~30min；The rotating speed of the centrifugation is 8000~30000r/
min。
5. extract the method without the residual mogroside of agriculture and water-soluble dietary fiber simultaneously according to one of Claims 1 to 4,
Characterized in that, step（3）In, in the alumina chromatographic column consumption of aluminum oxide for Momordica grosvenori fresh fruit quality 0.05~
1.00 again；The particle diameter of the aluminum oxide is 100~300 mesh；The ratio of height to diameter of the alumina chromatographic column is 1~12:1, upper prop
Flow velocity is 0.4~5.0BV/h.
6. extract the method without the residual mogroside of agriculture and water-soluble dietary fiber simultaneously according to one of Claims 1 to 5,
Characterized in that, step（3）In, in the activated carbon chromatography post consumption of activated carbon for Momordica grosvenori fresh fruit quality 0.05~
2.00 again；The particle diameter of the activated carbon is 10~500 mesh；The activated carbon is wood activated charcoal or coal mass active carbon；The work
Property charcoal chromatographic column ratio of height to diameter be 1~10:1, the flow velocity of upper prop is 0.4~5.0BV/h.
7. extract the method without the residual mogroside of agriculture and water-soluble dietary fiber simultaneously according to one of claim 1~6,
Characterized in that, step（4）In, the molecular cut off of the milipore filter is 1~50,000 Da, the pressure of filtering for 0.1~
1.2MPa。
8. extract the method without the residual mogroside of agriculture and water-soluble dietary fiber simultaneously according to one of claim 1~7,
Characterized in that, step（5）In, the molecular cut off of the NF membrane is 1000~5000Da, the pressure of filtering for 0.2~
2.0MPa。
9. extract the method without the residual mogroside of agriculture and water-soluble dietary fiber simultaneously according to one of claim 1~8,
Characterized in that, step（5）In, the vacuum being concentrated under reduced pressure is -0.06~-0.09MPa, and temperature is 65~80 DEG C, dense
It is 20~50% to be reduced to solid content；The drying is microwave drying, and the temperature of microwave drying is 60~80 DEG C, microwave drying it is true
Reciprocal of duty cycle is -0.05~-0.09MPa, and the time of microwave drying is 1~4h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710388435.1A CN107033209B (en) | 2017-05-27 | 2017-05-27 | A kind of while method of the extraction without agriculture residual mogroside and water-soluble dietary fiber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710388435.1A CN107033209B (en) | 2017-05-27 | 2017-05-27 | A kind of while method of the extraction without agriculture residual mogroside and water-soluble dietary fiber |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107033209A true CN107033209A (en) | 2017-08-11 |
| CN107033209B CN107033209B (en) | 2019-03-26 |
Family
ID=59539062
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710388435.1A Active CN107033209B (en) | 2017-05-27 | 2017-05-27 | A kind of while method of the extraction without agriculture residual mogroside and water-soluble dietary fiber |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107033209B (en) |
Cited By (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107602653A (en) * | 2017-10-09 | 2018-01-19 | 晨光生物科技集团股份有限公司 | A kind of method that mogroside is extracted from fresh fructus momordicae |
| CN108094772A (en) * | 2017-12-08 | 2018-06-01 | 桂林莱茵生物科技股份有限公司 | A kind of grosvenor momordica fruit juice and preparation method thereof |
| CN108185104A (en) * | 2018-04-08 | 2018-06-22 | 湖南艾达伦科技有限公司 | One kind smears tea mousse and preparation method |
| CN108184958A (en) * | 2018-04-08 | 2018-06-22 | 湖南艾达伦科技有限公司 | A kind of Cranberry biscuit and preparation method thereof |
| CN108208088A (en) * | 2018-04-08 | 2018-06-29 | 湖南艾达伦科技有限公司 | A kind of Ka Shida sauce and preparation method |
| CN108294142A (en) * | 2018-04-08 | 2018-07-20 | 湖南艾达伦科技有限公司 | A kind of light drink and preparation method thereof of black tea |
| CN108308323A (en) * | 2018-04-08 | 2018-07-24 | 湖南艾达伦科技有限公司 | A kind of light drink and preparation method thereof of green tea |
| CN108323665A (en) * | 2018-04-08 | 2018-07-27 | 湖南艾达伦科技有限公司 | A kind of light drink and preparation method thereof of Siraitia grosvenorii |
| CN108371268A (en) * | 2018-04-08 | 2018-08-07 | 湖南艾达伦科技有限公司 | A kind of Siraitia grosvenorii sweet orange beverage and preparation method thereof |
| CN108402129A (en) * | 2018-04-08 | 2018-08-17 | 湖南艾达伦科技有限公司 | A kind of Siraitia grosvenorii cake and preparation method thereof |
| CN108497248A (en) * | 2018-04-11 | 2018-09-07 | 湖南华诚生物资源股份有限公司 | A kind of preparation method of mangosteen juice and Siraitia grosvenorii fruit powder |
| CN108514010A (en) * | 2018-04-08 | 2018-09-11 | 湖南艾达伦科技有限公司 | A kind of puff and preparation method thereof |
| CN108514008A (en) * | 2018-04-08 | 2018-09-11 | 湖南艾达伦科技有限公司 | A kind of Siraitia grosvenorii toast and preparation method |
| CN108514011A (en) * | 2018-04-08 | 2018-09-11 | 湖南艾达伦科技有限公司 | A kind of vanilla butter sauce and preparation method thereof |
| CN108516996A (en) * | 2018-05-24 | 2018-09-11 | 湖南华诚生物资源股份有限公司 | The extracting method of Siraitia grosvenorii prebiotics and the method for extracting momordica glycoside V simultaneously |
| CN108606149A (en) * | 2018-04-08 | 2018-10-02 | 湖南艾达伦科技有限公司 | A kind of preparation method of Siraitia grosvenorii ice cream |
| CN108606122A (en) * | 2018-04-08 | 2018-10-02 | 湖南艾达伦科技有限公司 | A kind of light drink and preparation method thereof of Siraitia grosvenorii chrysanthemum |
| CN108606287A (en) * | 2018-04-08 | 2018-10-02 | 湖南艾达伦科技有限公司 | A kind of strawberry jam and preparation method thereof |
| CN109247561A (en) * | 2018-09-06 | 2019-01-22 | 湖南绿蔓生物科技股份有限公司 | A kind of method and its application preparing Siraitia grosvenorii sweetener composition from Siraitia grosvenorii |
| CN109320400A (en) * | 2018-09-30 | 2019-02-12 | 湖南华诚生物资源股份有限公司 | A method of natural mannitol is extracted from mogroside production waste liquid |
| CN110521993A (en) * | 2019-08-13 | 2019-12-03 | 湖南艾达伦科技有限公司 | Momordia grosvenori aglycone sweet taste and flavour compositions and the preparation method and application thereof |
| CN111543530A (en) * | 2020-06-22 | 2020-08-18 | 湖南绿果甜品有限公司 | Preparation method of low-calorie fructus momordicae sweet tea light drink |
| CN111588043A (en) * | 2020-06-05 | 2020-08-28 | 湖南华诚生物资源股份有限公司 | Preparation method of dietary fiber based on momordica grosvenori waste |
| CN114634545A (en) * | 2022-04-19 | 2022-06-17 | 湖南华诚生物资源股份有限公司 | Large-scale production method of mogroside with pesticide residues removed |
| CN115124585A (en) * | 2022-08-02 | 2022-09-30 | 湖南华诚生物资源股份有限公司 | Clean production method of momordica grosvenori extract |
| CN115804429A (en) * | 2021-09-13 | 2023-03-17 | 桂林莱茵生物科技股份有限公司 | Fructus momordicae sugar-reduced juice powder and preparation method thereof |
| WO2023173705A1 (en) * | 2022-03-16 | 2023-09-21 | 桂林莱茵生物科技股份有限公司 | Sweetening agent composition, preparation method therefor and use thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1683387A (en) * | 2005-03-16 | 2005-10-19 | 桂林莱茵生物科技股份有限公司 | Method for extracting momordica glycoside from momordica grosvenori |
| CN101007042A (en) * | 2007-02-06 | 2007-08-01 | 桂林莱茵生物科技股份有限公司 | A preparation method of debittered and discolored siraitia grosvenorii extracts |
| CN106306991A (en) * | 2016-08-23 | 2017-01-11 | 湖南华诚生物资源股份有限公司 | Method for removing pesticide residues and plasticizer from fructus momordicae extract at the same time |
| CN106617115A (en) * | 2017-02-20 | 2017-05-10 | 湖南华诚生物资源股份有限公司 | Method for separating soluble dietary fibers from Momordica grosvenori production waste liquid |
-
2017
- 2017-05-27 CN CN201710388435.1A patent/CN107033209B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1683387A (en) * | 2005-03-16 | 2005-10-19 | 桂林莱茵生物科技股份有限公司 | Method for extracting momordica glycoside from momordica grosvenori |
| CN101007042A (en) * | 2007-02-06 | 2007-08-01 | 桂林莱茵生物科技股份有限公司 | A preparation method of debittered and discolored siraitia grosvenorii extracts |
| CN106306991A (en) * | 2016-08-23 | 2017-01-11 | 湖南华诚生物资源股份有限公司 | Method for removing pesticide residues and plasticizer from fructus momordicae extract at the same time |
| CN106617115A (en) * | 2017-02-20 | 2017-05-10 | 湖南华诚生物资源股份有限公司 | Method for separating soluble dietary fibers from Momordica grosvenori production waste liquid |
Cited By (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107602653A (en) * | 2017-10-09 | 2018-01-19 | 晨光生物科技集团股份有限公司 | A kind of method that mogroside is extracted from fresh fructus momordicae |
| CN107602653B (en) * | 2017-10-09 | 2020-06-16 | 晨光生物科技集团股份有限公司 | Method for extracting mogroside from fresh momordica grosvenori |
| CN108094772A (en) * | 2017-12-08 | 2018-06-01 | 桂林莱茵生物科技股份有限公司 | A kind of grosvenor momordica fruit juice and preparation method thereof |
| CN108606122A (en) * | 2018-04-08 | 2018-10-02 | 湖南艾达伦科技有限公司 | A kind of light drink and preparation method thereof of Siraitia grosvenorii chrysanthemum |
| CN108294142A (en) * | 2018-04-08 | 2018-07-20 | 湖南艾达伦科技有限公司 | A kind of light drink and preparation method thereof of black tea |
| CN108606287A (en) * | 2018-04-08 | 2018-10-02 | 湖南艾达伦科技有限公司 | A kind of strawberry jam and preparation method thereof |
| CN108308323A (en) * | 2018-04-08 | 2018-07-24 | 湖南艾达伦科技有限公司 | A kind of light drink and preparation method thereof of green tea |
| CN108323665A (en) * | 2018-04-08 | 2018-07-27 | 湖南艾达伦科技有限公司 | A kind of light drink and preparation method thereof of Siraitia grosvenorii |
| CN108371268A (en) * | 2018-04-08 | 2018-08-07 | 湖南艾达伦科技有限公司 | A kind of Siraitia grosvenorii sweet orange beverage and preparation method thereof |
| CN108402129A (en) * | 2018-04-08 | 2018-08-17 | 湖南艾达伦科技有限公司 | A kind of Siraitia grosvenorii cake and preparation method thereof |
| CN108185104A (en) * | 2018-04-08 | 2018-06-22 | 湖南艾达伦科技有限公司 | One kind smears tea mousse and preparation method |
| CN108514010A (en) * | 2018-04-08 | 2018-09-11 | 湖南艾达伦科技有限公司 | A kind of puff and preparation method thereof |
| CN108514008A (en) * | 2018-04-08 | 2018-09-11 | 湖南艾达伦科技有限公司 | A kind of Siraitia grosvenorii toast and preparation method |
| CN108514011A (en) * | 2018-04-08 | 2018-09-11 | 湖南艾达伦科技有限公司 | A kind of vanilla butter sauce and preparation method thereof |
| CN108208088A (en) * | 2018-04-08 | 2018-06-29 | 湖南艾达伦科技有限公司 | A kind of Ka Shida sauce and preparation method |
| CN108606149A (en) * | 2018-04-08 | 2018-10-02 | 湖南艾达伦科技有限公司 | A kind of preparation method of Siraitia grosvenorii ice cream |
| CN108184958A (en) * | 2018-04-08 | 2018-06-22 | 湖南艾达伦科技有限公司 | A kind of Cranberry biscuit and preparation method thereof |
| CN108497248A (en) * | 2018-04-11 | 2018-09-07 | 湖南华诚生物资源股份有限公司 | A kind of preparation method of mangosteen juice and Siraitia grosvenorii fruit powder |
| CN108516996A (en) * | 2018-05-24 | 2018-09-11 | 湖南华诚生物资源股份有限公司 | The extracting method of Siraitia grosvenorii prebiotics and the method for extracting momordica glycoside V simultaneously |
| CN109247561A (en) * | 2018-09-06 | 2019-01-22 | 湖南绿蔓生物科技股份有限公司 | A kind of method and its application preparing Siraitia grosvenorii sweetener composition from Siraitia grosvenorii |
| CN109320400B (en) * | 2018-09-30 | 2021-08-10 | 湖南华诚生物资源股份有限公司 | Method for extracting natural mannitol from waste liquid of mogroside production |
| CN109320400A (en) * | 2018-09-30 | 2019-02-12 | 湖南华诚生物资源股份有限公司 | A method of natural mannitol is extracted from mogroside production waste liquid |
| CN110521993A (en) * | 2019-08-13 | 2019-12-03 | 湖南艾达伦科技有限公司 | Momordia grosvenori aglycone sweet taste and flavour compositions and the preparation method and application thereof |
| CN111588043A (en) * | 2020-06-05 | 2020-08-28 | 湖南华诚生物资源股份有限公司 | Preparation method of dietary fiber based on momordica grosvenori waste |
| CN111588043B (en) * | 2020-06-05 | 2023-11-10 | 邵阳华诚绿果生物科技有限公司 | Preparation method of dietary fiber based on Siraitia grosvenorii waste |
| CN111543530A (en) * | 2020-06-22 | 2020-08-18 | 湖南绿果甜品有限公司 | Preparation method of low-calorie fructus momordicae sweet tea light drink |
| CN111543530B (en) * | 2020-06-22 | 2023-06-02 | 湖南华诚生物资源股份有限公司 | Preparation method of low-calorie momordica grosvenori sweet tea light drink |
| CN115804429A (en) * | 2021-09-13 | 2023-03-17 | 桂林莱茵生物科技股份有限公司 | Fructus momordicae sugar-reduced juice powder and preparation method thereof |
| WO2023173705A1 (en) * | 2022-03-16 | 2023-09-21 | 桂林莱茵生物科技股份有限公司 | Sweetening agent composition, preparation method therefor and use thereof |
| CN114634545B (en) * | 2022-04-19 | 2023-02-24 | 湖南华诚生物资源股份有限公司 | Large-scale production method of mogroside with pesticide residues removed |
| CN114634545A (en) * | 2022-04-19 | 2022-06-17 | 湖南华诚生物资源股份有限公司 | Large-scale production method of mogroside with pesticide residues removed |
| CN115124585A (en) * | 2022-08-02 | 2022-09-30 | 湖南华诚生物资源股份有限公司 | Clean production method of momordica grosvenori extract |
| CN115124585B (en) * | 2022-08-02 | 2024-05-28 | 湖南华诚生物资源股份有限公司 | Clean production method of momordica grosvenori extract |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107033209B (en) | 2019-03-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107033209B (en) | A kind of while method of the extraction without agriculture residual mogroside and water-soluble dietary fiber | |
| CN103923152B (en) | A kind of extracting method of mogroside V | |
| CN104086614B (en) | A kind of preparation method being applicable to industrial Fructus Monordicae extract | |
| CN106588616B (en) | A kind of preparation method of curcumin | |
| Wang et al. | Separation and purification of amygdalin from thinned bayberry kernels by macroporous adsorption resins | |
| CN107235949A (en) | A kind of extracting method without plasticiser cyanidin extract | |
| CN106279339A (en) | A kind of isolation and purification method of high-purity Momordia grosvenori aglycone V | |
| CN101829210A (en) | Method for preparing apple polyphenol | |
| CN103446195B (en) | The preparation method of Folium Ginkgo extract | |
| CN105053952B (en) | A kind of processing technology of the dried orange peel extracts of no bitter taste | |
| CN107721967A (en) | A kind of green-tea extract production technology of high EGCG content | |
| CN104610417A (en) | Method for extracting ursolic acid and oleanolic acid from hawthorns | |
| CN104926719B (en) | A kind of method that trigonelline is extracted from fructus cannabis | |
| CN104892717B (en) | A kind of technical grade preparative liquid chromatography separation method of momordica glycoside V | |
| CN103880806A (en) | Method for extracting anthocyanin from blueberry | |
| CN1762428A (en) | Method for extracting efficient component from mulberry leaf | |
| CN107556284A (en) | The method that OPC is extracted from litchi rind | |
| CN104910223A (en) | Preparation method of oleuropein | |
| CN109021046B (en) | Method for simultaneously extracting quercetin and kaempferitrin from stem and leaf of momordica grosvenori | |
| CN102293791B (en) | Production method for extracting effective components of gingko leaf | |
| CN102133244B (en) | Preparation method of low-acid and high-quality ginkgo leaf extractive | |
| CN111297936A (en) | Method for extracting and separating total flavone, total triterpenoid saponin and total polysaccharide from momordica grosvenori roots | |
| CN105712896A (en) | Method for efficiently preparing macamides monomers from Lepidium meyenii | |
| CN113603742B (en) | Preparation method of mogroside V | |
| CN104610418A (en) | Method for extracting ursolic acid from cynomorium songaricum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A method for simultaneous extraction of Mogroside and water soluble dietary fiber from Siraitia grosvenorii Effective date of registration: 20210422 Granted publication date: 20190326 Pledgee: CITIC Bank Changsha branch Pledgor: HUNAN HUACHENG BIOTECH, Inc. Registration number: Y2021980002898 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20231127 Granted publication date: 20190326 Pledgee: CITIC Bank Changsha branch Pledgor: HUNAN HUACHENG BIOTECH, Inc. Registration number: Y2021980002898 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A method for simultaneous extraction of mogroside and water-soluble dietary fiber from Momordica grosvenorii without pesticide residues Granted publication date: 20190326 Pledgee: Agricultural Bank of China Limited Changsha Wangcheng District sub branch Pledgor: HUNAN HUACHENG BIOTECH, Inc. Registration number: Y2024980000707 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |