CN107029279A - A kind of high power water-absorbing antibacterial anti hemorrhagic uses sponge - Google Patents
A kind of high power water-absorbing antibacterial anti hemorrhagic uses sponge Download PDFInfo
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- CN107029279A CN107029279A CN201710133526.0A CN201710133526A CN107029279A CN 107029279 A CN107029279 A CN 107029279A CN 201710133526 A CN201710133526 A CN 201710133526A CN 107029279 A CN107029279 A CN 107029279A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0036—Porous materials, e.g. foams or sponges
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/0005—Ingredients of undetermined constitution or reaction products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0015—Medicaments; Biocides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/0047—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
- A61L24/0073—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material with a macromolecular matrix
- A61L24/0094—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material with a macromolecular matrix containing macromolecular fillers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/418—Agents promoting blood coagulation, blood-clotting agents, embolising agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
Abstract
A kind of high power water-absorbing antibacterial anti hemorrhagic of disclosure of the invention is made up with sponge of following component：Converted starch, collagen, chitosan Rabdosia rubescens complex, hydrogel.Styptic sponge prepared by the present invention can promote blood coagulation with wound tissue tight adhesion, and sludged blood does not stick together with the gauze dressing pressed, does not result in bleeding again.
Description
Technical field
The present invention relates to medical material technical field, more particularly to a kind of high power water-absorbing antibacterial anti hemorrhagic uses sponge.
Background technology
Extensive bleeding and oozing of blood are the problems for needing to solve in surgical operation and wound.
Collagen is that animal in-vivo content is most, is distributed most wide protein, is the major structural protein of body, is branch
Hold the key component of tissue and connective tissue, it have good biocompatibility, anthemorrhagic performance, promote neoblast formation function and
Cell adhesion.Recent studies indicate that, collagen can rapidly stop blooding in 10 seconds, and granulation can be promoted to grow and hinder
Mouth healing, prevents the generation of bleeding again.The toughness of simple gelfoam or collagen haemostatic sponge is poor, and without only
Pain effect, and antibacterial bacteriostatic effect is poor, and antibacterial stability is low.
The content of the invention
Sponge is used it is an object of the invention to provide a kind of high power water-absorbing antibacterial anti hemorrhagic.
The present invention is achieved through the following technical solutions：
A kind of high power water-absorbing antibacterial anti hemorrhagic is made up of following component by weight with sponge：Converted starch 60-75, collagen egg
White 10-15, chitosan-Rabdosia rubescens complex 3-5, hydrogel 6-8；
The chitosan-Rabdosia rubescens complex preparation method is：
1. use mass fraction to be dissolved for 1.0% hydrochloric acid solution chitosan, obtain the chitosan that mass fraction is 3.5%
Solution；
2. Rabdosia rubescens is rubbed, the snakeheaded fish skull powder of addition Rabdosia rubescens quality 1.5% adds buck and is stirred, and adds alkaline egg
White enzyme, at 52 DEG C, pH is under conditions of 8.5, to digest 3h, then temperature is down to 42 DEG C, and regulation pH is 6.4, adds Papain
Enzyme, 2h is reacted at 55 DEG C, and go out enzyme 5min, then adds nano diatomite, the amount of addition is Rabdosia rubescens quality 1.8%, adjusts pH
It is worth for 4.7, temperature is maintained at 52 DEG C of 5min, is then cooled down, obtains mixed liquor, adds the anhydrous second of mixed liquor quality 85%
Alcohol is precipitated, filtering, collects precipitation, and drying is crushed to 1100 mesh, produces Rabdosia rubescens extract；
3. Rabdosia rubescens extract and chitosan solution are pressed 1：3 mass ratios uniformly after mixing, are heated to 55 DEG C, are incubated 3h, from
Room temperature so is cooled to, is neutralized using sodium hydroxide, 1/5th of original volume is concentrated into, produces；
The method for preparing modified starch is：Cornstarch and soybean starch are pressed 5：1 mass ratio is weighed respectively, and corn is formed sediment
Powder is added in the sodium hydroxide solution that the mass fraction of 20 times of its quality is 0.5%, is tuned into slurry, is subsequently placed at 68 DEG C, is protected
Warm 30min, then 20min is stirred with 1500r/min rotating speeds, then by sodium hydroxide that epoxychloropropane and mass fraction are 0.5%
Solution presses 1：After 10 mass ratios are well mixed, mixed solution A is obtained, mixed solution A is pressed 1：6 mass ratios are added drop-wise to slurry
In, 72 DEG C are heated to, 2h is incubated, is then neutralized using hydrochloric acid, after standing 2 hours, filters, washs, drying is crosslinked
Cornstarch；Soybean starch is mixed with water, the soybean starch that mass fraction is 25% is modulated into and starches, then using mass fraction
For 2% sodium carbonate liquor regulation soybean starch slurry pH to 8.5, it is subsequently placed at 80 DEG C and is incubated 20min, then is slowly added increasing
The sodium hypochlorite of beans starch quality 5%, while adding hydrochloric acid regulation soybean starch slurry pH stabilizations after 6, reaction completely, was carried out
Filter, is washed, drying, obtains aoxidizing soybean starch；Uniformly admixed together, the addition by cross-linked corn starch and oxidation soybean starch
Aoxidize the albumen stone powder of soybean starch quality 2%, after stirring, after handle 20s in 250W microwaves, produce required denaturation
Starch.
Further, the collagen is soft-shelled turtle collagen.
A kind of high power water-absorbing antibacterial anti hemorrhagic preparation method of sponge, including：
(1) collagen is dissolved into the solution A that mass fraction is 1.5-1.8% with distilled water；
(2) solution B for being 1.2-1.6% into mass fraction with diluted hydrochloric acid dissolution by chitosan-Rabdosia rubescens complex, and use matter
Amount fraction is neutralized for 5% sodium carbonate liquor；
(3) solution A is mixed with solution B, then adds converted starch, hydrogel, 20min is stirred with 500r/min rotating speeds
Afterwards, use frequency to carry out processing 30s for 25KHz ultrasonic wave, then 2min is stirred with 1800r/min rotating speeds, then again through vacuum
Deaeration, is then incubated 10min at 2-4 DEG C, and sponge is formed after being then freeze-dried, using Co 60 illumination-based disinfection, you can.
The beneficial effects of the invention are as follows compared with prior art：The present invention is by respectively to cornstarch and soybean starch
The denaturation treatment of different modes is carried out, is then combined so that its water absorbent rate of the styptic sponge prepared and water suction speed
The sponge that rate is made far above single converted starch of the prior art, water absorbent rate averagely improves more than 40%, water suction speed
Rate improves more than 1.5 times, there is obvious performance advantage, by adding certain mass in cornstarch and soybean starch compound tense
Albumen stone flour, can further spread the structure of starch molecule, while enable between starch molecular structure framework to be molded,
Its water absorbing properties not only greatly improved, and stability is high so that sponge structure is porous and hole between be mutually communicated, sea
Soft good toughness, uniform pore diameter, air penetrability and water absorption rate are high；The present invention by chitosan-Rabdosia rubescens by carrying out compound obtained shell
Protonation ammonium in glycan-Rabdosia rubescens complex, obtained chitosan-Rabdosia rubescens complex molecule has electropositive, can inhale
It is accompanied with the bacterium of negative electrical charge so that the negative electrical charge skewness of bacteria cell wall and cell membrane, the synthesis of interference cell wall, from
And antibacterial effect can be reached, meanwhile, part chitosan-Rabdosia rubescens complex can adsorb bacterium, then penetrate porous thin
Enter intracellular in cell wall, form stable compound with DNA, the effect of interference archaeal dna polymerase or RNA polymerase hinders
DNA or RNA synthesis, so as to inhibit the breeding of bacterium, the purpose of antibacterial is reached with this；The present invention is ground by substantial amounts of experiment
Study carefully discovery, coating of the present invention shows the most to the inhibition of Escherichia coli, Candida albicans, staphylococcus aureus and mould
Write, also, fungi is substantially better than to the inhibition of bacterium.
Styptic sponge prepared by the present invention can promote blood coagulation, sludged blood and the gauze of pressing with wound tissue tight adhesion
Dressing does not stick together, and does not result in bleeding again.
Embodiment
Embodiment 1
A kind of high power water-absorbing antibacterial anti hemorrhagic is made up of following component by weight with sponge：Converted starch 60, collagen
10th, chitosan-Rabdosia rubescens complex 3, hydrogel 6；
The chitosan-Rabdosia rubescens complex preparation method is：
1. use mass fraction to be dissolved for 1.0% hydrochloric acid solution chitosan, obtain the chitosan that mass fraction is 3.5%
Solution；
2. Rabdosia rubescens is rubbed, the snakeheaded fish skull powder of addition Rabdosia rubescens quality 1.5% adds buck and is stirred, and adds alkaline egg
White enzyme, at 52 DEG C, pH is under conditions of 8.5, to digest 3h, then temperature is down to 42 DEG C, and regulation pH is 6.4, adds Papain
Enzyme, 2h is reacted at 55 DEG C, and go out enzyme 5min, then adds nano diatomite, the amount of addition is Rabdosia rubescens quality 1.8%, adjusts pH
It is worth for 4.7, temperature is maintained at 52 DEG C of 5min, is then cooled down, obtains mixed liquor, adds the anhydrous second of mixed liquor quality 85%
Alcohol is precipitated, filtering, collects precipitation, and drying is crushed to 1100 mesh, produces Rabdosia rubescens extract；
3. Rabdosia rubescens extract and chitosan solution are pressed 1：3 mass ratios uniformly after mixing, are heated to 55 DEG C, are incubated 3h, from
Room temperature so is cooled to, is neutralized using sodium hydroxide, 1/5th of original volume is concentrated into, produces；
The method for preparing modified starch is：Cornstarch and soybean starch are pressed 5：1 mass ratio is weighed respectively, and corn is formed sediment
Powder is added in the sodium hydroxide solution that the mass fraction of 20 times of its quality is 0.5%, is tuned into slurry, is subsequently placed at 68 DEG C, is protected
Warm 30min, then 20min is stirred with 1500r/min rotating speeds, then by sodium hydroxide that epoxychloropropane and mass fraction are 0.5%
Solution presses 1：After 10 mass ratios are well mixed, mixed solution A is obtained, mixed solution A is pressed 1：6 mass ratios are added drop-wise to slurry
In, 72 DEG C are heated to, 2h is incubated, is then neutralized using hydrochloric acid, after standing 2 hours, filters, washs, drying is crosslinked
Cornstarch；Soybean starch is mixed with water, the soybean starch that mass fraction is 25% is modulated into and starches, then using mass fraction
For 2% sodium carbonate liquor regulation soybean starch slurry pH to 8.5, it is subsequently placed at 80 DEG C and is incubated 20min, then is slowly added increasing
The sodium hypochlorite of beans starch quality 5%, while adding hydrochloric acid regulation soybean starch slurry pH stabilizations after 6, reaction completely, was carried out
Filter, is washed, drying, obtains aoxidizing soybean starch；Uniformly admixed together, the addition by cross-linked corn starch and oxidation soybean starch
Aoxidize the albumen stone powder of soybean starch quality 2%, after stirring, after handle 20s in 250W microwaves, produce required denaturation
Starch.
The collagen is soft-shelled turtle collagen.
A kind of high power water-absorbing antibacterial anti hemorrhagic preparation method of sponge, including：
(1) collagen is dissolved into the solution A that mass fraction is 1.5% with distilled water；
(2) chitosan-Rabdosia rubescens complex diluted hydrochloric acid dissolution is divided into the solution B that mass fraction is 1.2%, and use quality
Number is neutralized for 5% sodium carbonate liquor；
(3) solution A is mixed with solution B, then adds converted starch, hydrogel, 20min is stirred with 500r/min rotating speeds
Afterwards, use frequency to carry out processing 30s for 25KHz ultrasonic wave, then 2min is stirred with 1800r/min rotating speeds, then again through vacuum
Deaeration, is then incubated 10min at 2 DEG C, and sponge is formed after being then freeze-dried, using Co 60 illumination-based disinfection, you can.
Embodiment 2
A kind of high power water-absorbing antibacterial anti hemorrhagic is made up of following component by weight with sponge：Converted starch 75, collagen
15th, chitosan-Rabdosia rubescens complex 5, hydrogel 8；
The chitosan-Rabdosia rubescens complex preparation method is：
1. use mass fraction to be dissolved for 1.0% hydrochloric acid solution chitosan, obtain the chitosan that mass fraction is 3.5%
Solution；
2. Rabdosia rubescens is rubbed, the snakeheaded fish skull powder of addition Rabdosia rubescens quality 1.5% adds buck and is stirred, and adds alkaline egg
White enzyme, at 52 DEG C, pH is under conditions of 8.5, to digest 3h, then temperature is down to 42 DEG C, and regulation pH is 6.4, adds Papain
Enzyme, 2h is reacted at 55 DEG C, and go out enzyme 5min, then adds nano diatomite, the amount of addition is Rabdosia rubescens quality 1.8%, adjusts pH
It is worth for 4.7, temperature is maintained at 52 DEG C of 5min, is then cooled down, obtains mixed liquor, adds the anhydrous second of mixed liquor quality 85%
Alcohol is precipitated, filtering, collects precipitation, and drying is crushed to 1100 mesh, produces Rabdosia rubescens extract；
3. Rabdosia rubescens extract and chitosan solution are pressed 1：3 mass ratios uniformly after mixing, are heated to 55 DEG C, are incubated 3h, from
Room temperature so is cooled to, is neutralized using sodium hydroxide, 1/5th of original volume is concentrated into, produces；
The method for preparing modified starch is：Cornstarch and soybean starch are pressed 5：1 mass ratio is weighed respectively, and corn is formed sediment
Powder is added in the sodium hydroxide solution that the mass fraction of 20 times of its quality is 0.5%, is tuned into slurry, is subsequently placed at 68 DEG C, is protected
Warm 30min, then 20min is stirred with 1500r/min rotating speeds, then by sodium hydroxide that epoxychloropropane and mass fraction are 0.5%
Solution presses 1：After 10 mass ratios are well mixed, mixed solution A is obtained, mixed solution A is pressed 1：6 mass ratios are added drop-wise to slurry
In, 72 DEG C are heated to, 2h is incubated, is then neutralized using hydrochloric acid, after standing 2 hours, filters, washs, drying is crosslinked
Cornstarch；Soybean starch is mixed with water, the soybean starch that mass fraction is 25% is modulated into and starches, then using mass fraction
For 2% sodium carbonate liquor regulation soybean starch slurry pH to 8.5, it is subsequently placed at 80 DEG C and is incubated 20min, then is slowly added increasing
The sodium hypochlorite of beans starch quality 5%, while adding hydrochloric acid regulation soybean starch slurry pH stabilizations after 6, reaction completely, was carried out
Filter, is washed, drying, obtains aoxidizing soybean starch；Uniformly admixed together, the addition by cross-linked corn starch and oxidation soybean starch
Aoxidize the albumen stone powder of soybean starch quality 2%, after stirring, after handle 20s in 250W microwaves, produce required denaturation
Starch.
The collagen is soft-shelled turtle collagen.
A kind of high power water-absorbing antibacterial anti hemorrhagic preparation method of sponge, including：
(1) collagen is dissolved into the solution A that mass fraction is 1.8% with distilled water；
(2) chitosan-Rabdosia rubescens complex diluted hydrochloric acid dissolution is divided into the solution B that mass fraction is 1.6%, and use quality
Number is neutralized for 5% sodium carbonate liquor；
(3) solution A is mixed with solution B, then adds converted starch, hydrogel, 20min is stirred with 500r/min rotating speeds
Afterwards, use frequency to carry out processing 30s for 25KHz ultrasonic wave, then 2min is stirred with 1800r/min rotating speeds, then again through vacuum
Deaeration, is then incubated 10min at 4 DEG C, and sponge is formed after being then freeze-dried, using Co 60 illumination-based disinfection, you can.
Embodiment 3
A kind of high power water-absorbing antibacterial anti hemorrhagic is made up of following component by weight with sponge：Converted starch 68, collagen
12nd, chitosan-Rabdosia rubescens complex 4, hydrogel 7；
The chitosan-Rabdosia rubescens complex preparation method is：
1. use mass fraction to be dissolved for 1.0% hydrochloric acid solution chitosan, obtain the chitosan that mass fraction is 3.5%
Solution；
2. Rabdosia rubescens is rubbed, the snakeheaded fish skull powder of addition Rabdosia rubescens quality 1.5% adds buck and is stirred, and adds alkaline egg
White enzyme, at 52 DEG C, pH is under conditions of 8.5, to digest 3h, then temperature is down to 42 DEG C, and regulation pH is 6.4, adds Papain
Enzyme, 2h is reacted at 55 DEG C, and go out enzyme 5min, then adds nano diatomite, the amount of addition is Rabdosia rubescens quality 1.8%, adjusts pH
It is worth for 4.7, temperature is maintained at 52 DEG C of 5min, is then cooled down, obtains mixed liquor, adds the anhydrous second of mixed liquor quality 85%
Alcohol is precipitated, filtering, collects precipitation, and drying is crushed to 1100 mesh, produces Rabdosia rubescens extract；
3. Rabdosia rubescens extract and chitosan solution are pressed 1：3 mass ratios uniformly after mixing, are heated to 55 DEG C, are incubated 3h, from
Room temperature so is cooled to, is neutralized using sodium hydroxide, 1/5th of original volume is concentrated into, produces；
The method for preparing modified starch is：Cornstarch and soybean starch are pressed 5：1 mass ratio is weighed respectively, and corn is formed sediment
Powder is added in the sodium hydroxide solution that the mass fraction of 20 times of its quality is 0.5%, is tuned into slurry, is subsequently placed at 68 DEG C, is protected
Warm 30min, then 20min is stirred with 1500r/min rotating speeds, then by sodium hydroxide that epoxychloropropane and mass fraction are 0.5%
Solution presses 1：After 10 mass ratios are well mixed, mixed solution A is obtained, mixed solution A is pressed 1：6 mass ratios are added drop-wise to slurry
In, 72 DEG C are heated to, 2h is incubated, is then neutralized using hydrochloric acid, after standing 2 hours, filters, washs, drying is crosslinked
Cornstarch；Soybean starch is mixed with water, the soybean starch that mass fraction is 25% is modulated into and starches, then using mass fraction
For 2% sodium carbonate liquor regulation soybean starch slurry pH to 8.5, it is subsequently placed at 80 DEG C and is incubated 20min, then is slowly added increasing
The sodium hypochlorite of beans starch quality 5%, while adding hydrochloric acid regulation soybean starch slurry pH stabilizations after 6, reaction completely, was carried out
Filter, is washed, drying, obtains aoxidizing soybean starch；Uniformly admixed together, the addition by cross-linked corn starch and oxidation soybean starch
Aoxidize the albumen stone powder of soybean starch quality 2%, after stirring, after handle 20s in 250W microwaves, produce required denaturation
Starch.
The collagen is soft-shelled turtle collagen.
A kind of high power water-absorbing antibacterial anti hemorrhagic preparation method of sponge, including：
(1) collagen is dissolved into the solution A that mass fraction is 1.7% with distilled water；
(2) chitosan-Rabdosia rubescens complex diluted hydrochloric acid dissolution is divided into the solution B that mass fraction is 1.4%, and use quality
Number is neutralized for 5% sodium carbonate liquor；
(3) solution A is mixed with solution B, then adds converted starch, hydrogel, 20min is stirred with 500r/min rotating speeds
Afterwards, use frequency to carry out processing 30s for 25KHz ultrasonic wave, then 2min is stirred with 1800r/min rotating speeds, then again through vacuum
Deaeration, is then incubated 10min at 3 DEG C, and sponge is formed after being then freeze-dried, using Co 60 illumination-based disinfection, you can.
Sterilize stability experiment（37 DEG C of accelerated tests）：
Test group 1：It is 1.4% chitosan-Rabdosia rubescens cooperation that chitosan-Rabdosia rubescens complex is mixed with water and is made into mass fraction
Thing solution；
Test group 2：Rabdosia rubescens extract is mixed with water and is made into mass fraction for 1.4% Rabdosia rubescens extract solution；
Test group 3：Chitosan is subjected to diluted acid dissolving, then neutralized, mass fraction is configured to for 1.4% chitosan solution；
Table 1
As can be seen from Table 1, the chitosan-Rabdosia rubescens complex added in the present invention
With fabulous antibacterial effect, and bactericidal effect lasting stability.
3 prepared by 3 kinds of styptic sponges made from the method for embodiment 1,2,3 and the method for comparative example 1,2,3 stop the sea of blood
Silk floss, its water absorbent rate and rate of water absorption contrast are as shown in the table：
Comparative example 1：The cross-linked corn starch that converted starch in the application is used to equivalent is differed only in embodiment 1, it is made
Preparation Method is identical with cross-linked corn starch preparation method in the application；
Comparative example 2：The oxidation soybean starch that converted starch in the application is used to equivalent is differed only in embodiment 1, it is made
Preparation Method is identical with aoxidizing soybean starch preparation method in the application；
Comparative example 3：The secondary cross-linking cornstarch that the converted starch of the application is used to equivalent is differed only in embodiment 1,
Its preparation method is that the cross-linked corn starch in the application is carried out into crosslinking Treatment again；
Table 2
As can be seen from Table 2, its water absorbent rate of styptic sponge and rate of water absorption that prepared by the present invention are far above in the prior art
Modified starch sponges.
Haemostatic effect of the styptic sponge to liver Hemorrhage Model
Test method：
Liver placenta percreta area 1.5cm × 2.5cm is cut with scalpel in pig liver surface, Wound depth is 0.25cm, is made
The bleeding surface of a wound, the styptic sponge of use has：Embodiment 1,2,3.
The above-mentioned surface of a wound is stopped blooding respectively.After bleeding, styptic sponge is placed on wound and with medical outer immediately
Section's gloves or hemostatic gauze press on sponge, blocking blood flow, gently unclamp gloves or gauze after 1~2min, observation hemostasis
Effect, gloves and gauze whether adhesion sponge and sludged blood, bleeding again is caused when opening.Without converted starch is extra large after hemostasis
Silk floss is taken away or removed, but appropriate infiltration is made of physiological saline and is rinsed.
Result of the test：
Very well, all styptic sponges are met sucks blood and forms viscosity with blood the sponge haemostatic effect of embodiment 1,2,3 immediately after blood
Sponge-sludged blood colloid, can efficiently control the bleeding of the liver surface of a wound within 40s.
Claims (3)
1. a kind of high power water-absorbing antibacterial anti hemorrhagic is with sponge, it is characterised in that be made up by weight of following component：Converted starch
60-75, collagen 10-15, chitosan-Rabdosia rubescens complex 3-5, hydrogel 6-8；
The chitosan-Rabdosia rubescens complex preparation method is：
1. use mass fraction to be dissolved for 1.0% hydrochloric acid solution chitosan, obtain the chitosan that mass fraction is 3.5%
Solution；
2. Rabdosia rubescens is rubbed, the snakeheaded fish skull powder of addition Rabdosia rubescens quality 1.5% adds buck and is stirred, and adds alkaline egg
White enzyme, at 52 DEG C, pH is under conditions of 8.5, to digest 3h, then temperature is down to 42 DEG C, and regulation pH is 6.4, adds Papain
Enzyme, 2h is reacted at 55 DEG C, and go out enzyme 5min, then adds nano diatomite, the amount of addition is Rabdosia rubescens quality 1.8%, adjusts pH
It is worth for 4.7, temperature is maintained at 52 DEG C of 5min, is then cooled down, obtains mixed liquor, adds the anhydrous second of mixed liquor quality 85%
Alcohol is precipitated, filtering, collects precipitation, and drying is crushed to 1100 mesh, produces Rabdosia rubescens extract；
3. Rabdosia rubescens extract and chitosan solution are pressed 1：3 mass ratios uniformly after mixing, are heated to 55 DEG C, are incubated 3h, from
Room temperature so is cooled to, is neutralized using sodium hydroxide, 1/5th of original volume is concentrated into, produces；
The method for preparing modified starch is：Cornstarch and soybean starch are pressed 5：1 mass ratio is weighed respectively, and corn is formed sediment
Powder is added in the sodium hydroxide solution that the mass fraction of 20 times of its quality is 0.5%, is tuned into slurry, is subsequently placed at 68 DEG C, is protected
Warm 30min, then 20min is stirred with 1500r/min rotating speeds, then by sodium hydroxide that epoxychloropropane and mass fraction are 0.5%
Solution presses 1：After 10 mass ratios are well mixed, mixed solution A is obtained, mixed solution A is pressed 1：6 mass ratios are added drop-wise to slurry
In, 72 DEG C are heated to, 2h is incubated, is then neutralized using hydrochloric acid, after standing 2 hours, filters, washs, drying is crosslinked
Cornstarch；Soybean starch is mixed with water, the soybean starch that mass fraction is 25% is modulated into and starches, then using mass fraction
PH is starched to 8.5 for 2% sodium carbonate liquor regulation soybean starch, is subsequently placed at 80 DEG C and is incubated 20min, then is slowly added increasing
The sodium hypochlorite of beans starch quality 5%, while adding hydrochloric acid regulation soybean starch slurry pH stabilizations after 6, reaction completely, was carried out
Filter, is washed, drying, obtains aoxidizing soybean starch；Uniformly admixed together, the addition by cross-linked corn starch and oxidation soybean starch
Aoxidize the albumen stone powder of soybean starch quality 2%, after stirring, after handle 20s in 250W microwaves, produce required denaturation
Starch.
2. a kind of high power water-absorbing antibacterial anti hemorrhagic according to claim 1 is with sponge, it is characterised in that the collagen is
Soft-shelled turtle collagen.
3. a kind of high power water-absorbing antibacterial anti hemorrhagic according to claim 1 or 2 preparation method of sponge, it is characterised in that
Including：
(1) collagen is dissolved into the solution A that mass fraction is 1.5-1.8% with distilled water；
(2) solution B for being 1.2-1.6% into mass fraction with diluted hydrochloric acid dissolution by chitosan-Rabdosia rubescens complex, and use matter
Amount fraction is neutralized for 5% sodium carbonate liquor；
(3) solution A is mixed with solution B, then adds converted starch, hydrogel, 20min is stirred with 500r/min rotating speeds
Afterwards, use frequency to carry out processing 30s for 25KHz ultrasonic wave, then 2min is stirred with 1800r/min rotating speeds, then again through vacuum
Deaeration, is then incubated 10min at 2-4 DEG C, and sponge is formed after being then freeze-dried, using Co 60 illumination-based disinfection, you can.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485897A (en) * | 2008-01-14 | 2009-07-22 | 纪欣 | Biocompatible hemostatic, antiblocking, healing-promoting and surgical wound-closing modified starch material |
| CN101695581A (en) * | 2009-10-26 | 2010-04-21 | 西北大学 | Method for preparing human-like collagen haemostatic sponge in scale |
| CN101785873A (en) * | 2009-01-23 | 2010-07-28 | 纪欣 | Biocompatible bleed-stopping and bone healing promoting material and preparation method thereof |
| CN106867344A (en) * | 2017-03-08 | 2017-06-20 | 安徽省伟业净化设备有限公司 | A kind of endoscope surface antibiotic paint |
| CN106860908A (en) * | 2017-03-08 | 2017-06-20 | 安徽省伟业净化设备有限公司 | A kind of efficient styptic sponge |
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2017
- 2017-03-08 CN CN201710133526.0A patent/CN107029279A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485897A (en) * | 2008-01-14 | 2009-07-22 | 纪欣 | Biocompatible hemostatic, antiblocking, healing-promoting and surgical wound-closing modified starch material |
| CN101785873A (en) * | 2009-01-23 | 2010-07-28 | 纪欣 | Biocompatible bleed-stopping and bone healing promoting material and preparation method thereof |
| CN101695581A (en) * | 2009-10-26 | 2010-04-21 | 西北大学 | Method for preparing human-like collagen haemostatic sponge in scale |
| CN106867344A (en) * | 2017-03-08 | 2017-06-20 | 安徽省伟业净化设备有限公司 | A kind of endoscope surface antibiotic paint |
| CN106860908A (en) * | 2017-03-08 | 2017-06-20 | 安徽省伟业净化设备有限公司 | A kind of efficient styptic sponge |
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