CN107027605A - A kind of method of half live body culture assay cherry chilling requirement - Google Patents
A kind of method of half live body culture assay cherry chilling requirement Download PDFInfo
- Publication number
- CN107027605A CN107027605A CN201710316948.1A CN201710316948A CN107027605A CN 107027605 A CN107027605 A CN 107027605A CN 201710316948 A CN201710316948 A CN 201710316948A CN 107027605 A CN107027605 A CN 107027605A
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- Prior art keywords
- cherry
- branch
- chilling requirement
- live body
- temperature
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
Abstract
The invention provides a kind of method of half live body culture assay cherry chilling requirement, including collection cherry branch；Low- temperature culture cherry branch；Incubated at room temperature cherry branch, statistics bud and leaf bud germination rate, determine cherry chilling requirement, and the method for the collection cherry branch at least leaves 5 buds to cut flat on branch base portion, control 30~50cm of branch length, and every branch.The present invention is by Low- temperature culture and incubated at room temperature alternate application, and simulated environment as far as possible has efficiently controlled temperature, it is to avoid branch is influenceed by ambient temperature uncontrollable factor or records ambient temperature using miscellaneous equipment, provides and facilitates for the calculating of chilling requirement.
Description
Technical field
The invention belongs to field of plant breeding, and in particular to a kind of method of half live body culture assay cherry chilling requirement.
Background technology
Chilling requirement refers to number during effective low temperature needed for deciduous fruit tree natural dormancy, and most deciduous fruit trees need to accumulate certain
Chilling requirement after could breaking dormancy, heated up.Chilling requirement becomes to deciduous fruit tree suitable planting, facility cultivation and prediction weather
Change has great importance.
Cherry fruit appearance is bright in colour, sparkling and crystal-clear beauty, a variety of rich in sugar, protein, vitamin and calcium, iron, phosphorus, potassium etc.
Element, is a kind of high-grade fruit, and economic value is high, is adapted to China's kind value.The maturity period of fruit can be done sth. in advance by facility cultivation,
So as to significantly increase economic benefit, but cherry as other fruit trees, it is necessary to accumulate after certain chilling requirement can just break from
Right dormancy, so as to normally yield positive results.Therefore, the chilling requirement of each kind of cherry is only known, could with correctly selecting facility
Cultivar and overlay film warm-up time.
Existing measurement fruit tree chilling requirement method is directly to gather branch from field at regular intervals after falling leaves mostly, then
Take back laboratory carry out illumination cultivation, observation bud or leaf bud germination rate, then according to local weather bureau monitor temperature or
The temperature that automonitor is determined is calculated, and this method consuming time is long, and branch is easily by ambient temperature uncontrollable factor
Influence, while need using miscellaneous equipment record ambient temperature.(Liu Rendao, Liu Jianjun sweet cherries different cultivars is needed Liu Rendao
The northern gardening of cold research, 2009 (2)：It is directly placed into 4 DEG C of refrigerators and preserves after 84-85) etc. branch is gathered, then carries out
Incubated at room temperature, but the shortcoming of the method is that withered or Signs of Mould occurs in branch storage for a long time, and deposited the beginning in refrigerator
Dark surrounds is in eventually, has certain influence on bud differentiation, it is impossible to accurately determine its chilling requirement.
The content of the invention
The present invention determines the problem of existing for current fruit tree chilling requirement, proposes that one kind utilizes half live body culture mensuration,
In addition to controlling temperature, environmental test condition as far as possible, more precisely to determine the chilling requirement of cherry, for production
Practice provides more structurally sound data.This method can be used for the chilling requirement of other deciduous fruit trees (peach, pears etc.) to determine, or its
The chilling requirement of his plant, which is determined, provides reference.
That is, the first object of the present invention is to provide a kind of method of half live body culture assay cherry chilling requirement, including with
Lower step：
1) cherry branch is gathered；
2) Low- temperature culture cherry branch；
3) incubated at room temperature cherry branch, statistics bud and leaf bud germination rate, determine cherry chilling requirement.
Preferably, in the method for half live body culture assay cherry chilling requirement of the present invention, the step 1) middle collection
Cherry branch be mid or late December cherry tree dormancy after, collection with full bud cherry tree on annotinous branch.
Preferably, in the method for half live body culture assay cherry chilling requirement of the present invention, the step 1) middle collection
The method of cherry branch at least leaves 5 flowers to cut flat on branch base portion, control 30~50cm of branch length, and every branch
Bud.
Preferably, in the method for half live body culture assay cherry chilling requirement of the present invention, the step 2) in low temperature
The method of culture is that the branch insertion of collection is equipped with the container of nutrient solution, is put into 4~7 DEG C of low temperature incubators and cultivates.
It is highly preferred that in the method for half live body culture assay cherry chilling requirement of the present invention, the step 2) in it is low
The illumination condition of temperature culture is day/night=10h/14h, intensity of illumination 1200LX.
Preferably, in the method for half live body culture assay cherry chilling requirement of the present invention, the step 3) in room temperature
The method for cultivating cherry branch is to take out the branch in low temperature incubator every 4 days, cuts off branch base portion, reveals branch bottom
Go out in fresh color, container of the insertion equipped with nutrient solution, the container that then will be equipped with branch is put into 25 DEG C of day temperature, evening temperature
Culture 15 days is carried out in 15 DEG C of incubator.
It is highly preferred that in the method for half live body culture assay cherry chilling requirement of the present invention, the incubated at room temperature
Illumination condition is day/night=14h/10h, intensity of illumination 2500LX.
Preferably, in the method for half live body culture assay cherry chilling requirement of the present invention, what the chilling requirement was determined
Standard is to reach that 50% is leaf bud and bud meets its own chilling requirement with leaf bud and bud germination rate, then with chilling requirement model meter
Calculate cherry chilling requirement.
Preferably, in the method for half live body culture assay cherry chilling requirement of the present invention, the nutrient solution is 25g/L
Sucrose solution；The nutrient solution is changed once for every 5 days during whole culture.
Compared with prior art, the present invention at least has advantages below to the present invention：
By Low- temperature culture and incubated at room temperature alternate application, simulated environment as far as possible has efficiently controlled temperature, kept away
Exempting from branch is influenceed by ambient temperature uncontrollable factor or records ambient temperature using miscellaneous equipment, is provided for the calculating of chilling requirement
It is convenient.
Embodiment
Below in conjunction with the embodiment in the present invention, the technical scheme in the present invention is clearly and completely described.It is aobvious
So, described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Based on the reality in the present invention
Example is applied, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made all belongs to
In the scope of protection of the invention.
Embodiment 1
1) branch is gathered：In mid-December short handle cherry tree and black pearl cherry tree fallen leaves after, collection carry full bud
Annotinous branch, cut flat branch base portion, control branch length 50cm, it is ensured that 5 buds are at least left on every branch.
2) Low- temperature culture：By in container of the branch insertion equipped with 25g/L sucrose nutrient solutions of collection, 4 DEG C of incubators are put into
It is middle to be cultivated, illumination condition day/night=10h/14h, intensity of illumination 1200LX.Nutrient solution was changed once every 5 days.
3) incubated at room temperature：The branch in 4 DEG C of incubators is taken out every 4 days, branch base portion is cut off, exposes branch bottom
Fresh color, insertion equipped with 25g/L sucrose nutrient solutions container in, then will be equipped with branch container be put into 25 DEG C of day temperature,
15 DEG C of evening temperature, day/night=14h/10h, intensity of illumination for 2500LX incubator in cultivated.After 15 days, system
Bud and leaf bud germination rate are counted, reaches that 50% is that leaf bud and bud meet its own chilling requirement with leaf bud and bud germination rate, then
Cherry chilling requirement is calculated with chilling requirement model.
The germination rate (%) of short handle and black pearl cherry leaf bud and bud after 14 DEG C of cultures of table
| Incubation time | 144h | 240h | 336h | 432h | 528h | 624h | 720h | 816h |
| Short handle leaf bud | 16.0 | 30.0 | 45.0 | 79.0 | 100 | |||
| Black pearl leaf bud | 10.0 | 15.0 | 20.0 | 24.0 | 35.0 | 55.0 | 66.7 | 100 |
| Short handle bud | 26.0 | 38.0 | 69.0 | 100 | ||||
| Black pearl bud | 10.0 | 22.0 | 36.0 | 44.0 | 72.7 | 100 |
As can be seen from Table 1, the chilling requirement of short handle leaf bud is 432h, and bud chilling requirement is 336h；Black pearl leaf bud needs cold
Measure as 624h, bud chilling requirement is 528h.
Embodiment 2
1) branch is gathered：In mid-December short handle and black pearl cherry tree fallen leaves after, collection was with 1 year of full bud
Raw branch, cuts flat branch base portion, control branch length 50cm, it is ensured that 5 buds are at least left on every branch.
2) Low- temperature culture：By in container of the branch insertion equipped with 25g/L sucrose nutrient solutions of collection, 7 DEG C of incubators are put into
It is middle to be cultivated, illumination condition day/night=10h/14h, intensity of illumination 1200LX.Nutrient solution was changed once every 5 days.
3) incubated at room temperature：The branch in 7 DEG C of incubators is taken out every 4 days, branch base portion is cut off, exposes branch bottom
Fresh color, insertion equipped with 25g/L sucrose nutrient solutions container in, then will be equipped with branch container be put into 25 DEG C of day temperature,
15 DEG C of evening temperature, day/night=14h/10h, intensity of illumination for 2500LX incubator in cultivated.After 15 days, system
Bud and leaf bud germination rate are counted, reaches that 50% is that leaf bud and bud meet its own chilling requirement with leaf bud and bud germination rate, then
Cherry chilling requirement is calculated with chilling requirement model.
The germination rate (%) of short handle and black pearl cherry leaf bud and bud after 27 DEG C of cultures of table
As can be seen from Table 2, the chilling requirement of short handle leaf bud is 432h, and bud chilling requirement is 336h；Black pearl leaf bud needs cold
Measure as 624h, bud chilling requirement is 528h.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of method of half live body culture assay cherry chilling requirement, it is characterised in that comprise the following steps：
1) cherry branch is gathered；
2) Low- temperature culture cherry branch；
3) incubated at room temperature cherry branch, statistics bud and leaf bud germination rate, determine cherry chilling requirement.
2. the method for half live body culture assay cherry chilling requirement according to claim 1, it is characterised in that the step 1)
The cherry branch of middle collection be mid or late December cherry tree dormancy after, collection with full bud cherry tree cherry branch.
3. the method for half live body culture assay cherry chilling requirement according to claim 1, it is characterised in that the step 1)
The method of middle collection cherry branch at least leaves 5 flowers to cut flat on branch base portion, control branch length 50cm, and every branch
Bud.
4. the method for half live body culture assay cherry chilling requirement according to claim 1, it is characterised in that the step 2)
The method of middle Low- temperature culture is that the branch insertion of collection is equipped with the container of nutrient solution, is put into 4 DEG C of incubators and cultivates.
5. the method for half live body culture assay cherry chilling requirement according to claim 4, it is characterised in that the step 2)
The illumination condition of middle Low- temperature culture：Cryogenic temperature is 4-7 degree, day/night=10h/14h, intensity of illumination 1200LX.
6. the method for half live body culture assay cherry chilling requirement according to claim 1, it is characterised in that the step 3)
The method of middle incubated at room temperature cherry branch is to take out the branch in low temperature incubator every 4 days, cuts off branch base portion, makes branch
Bottom is exposed in fresh color, container of the insertion equipped with nutrient solution, and the container that then will be equipped with branch is put into 25 DEG C of day temperature, evening
Culture 15 days is carried out in the incubator of upper 15 DEG C of temperature.
7. the method for half live body culture assay cherry chilling requirement according to claim 6, it is characterised in that the room temperature training
Foster illumination condition is day/night=14h/10h, intensity of illumination 2500LX.
8. the method for half live body culture assay cherry chilling requirement according to claim 1, it is characterised in that the chilling requirement
The standard of measure is to reach that 50% is that leaf bud and bud meet its own chilling requirement with leaf bud and bud germination rate, then uses chilling requirement
Model calculates cherry chilling requirement.
9. the method for the half live body culture assay cherry chilling requirement according to claim 4~8 any one, its feature exists
In the nutrient solution is 25g/L sucrose solution.
10. the method for half live body culture assay cherry chilling requirement according to claim 9, it is characterised in that the culture
Liquid is changed once for every 5 days during whole culture.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710316948.1A CN107027605B (en) | 2017-05-08 | 2017-05-08 | Method for measuring refrigerating capacity required by cherries through semi-living body culture |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710316948.1A CN107027605B (en) | 2017-05-08 | 2017-05-08 | Method for measuring refrigerating capacity required by cherries through semi-living body culture |
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| CN107027605A true CN107027605A (en) | 2017-08-11 |
| CN107027605B CN107027605B (en) | 2020-09-01 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112067746A (en) * | 2020-08-20 | 2020-12-11 | 广西特色作物研究院 | Method for measuring cold demand of peaches by living body |
Citations (2)
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| CN103176491A (en) * | 2013-03-13 | 2013-06-26 | 山东省果树研究所 | Automatic detecting, selecting and recording display system of cooling capacity required in facility cultivation of sweet cherry |
| CN104041368A (en) * | 2014-06-13 | 2014-09-17 | 四川省农业科学院园艺研究所 | Method for fast detecting cooling capacity needed by peaches |
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2017
- 2017-05-08 CN CN201710316948.1A patent/CN107027605B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103176491A (en) * | 2013-03-13 | 2013-06-26 | 山东省果树研究所 | Automatic detecting, selecting and recording display system of cooling capacity required in facility cultivation of sweet cherry |
| CN104041368A (en) * | 2014-06-13 | 2014-09-17 | 四川省农业科学院园艺研究所 | Method for fast detecting cooling capacity needed by peaches |
Non-Patent Citations (5)
| Title |
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| 冯建荣,康喜亮,樊新民: ""新疆主栽葡萄品种需冷量的研究"", 《中外葡萄与葡萄酒》 * |
| 刘仁道,刘建军: ""甜樱桃不同品种需冷量研究"", 《北方园艺》 * |
| 杨义伶,高洁等: ""猕猴桃不同品种需冷量的研究"", 《中国南方果树》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112067746A (en) * | 2020-08-20 | 2020-12-11 | 广西特色作物研究院 | Method for measuring cold demand of peaches by living body |
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| CN107027605B (en) | 2020-09-01 |
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