CN106172011A - A kind of dwarf banana high temperature light culture tissue culture and rapid propagation method - Google Patents
A kind of dwarf banana high temperature light culture tissue culture and rapid propagation method Download PDFInfo
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- CN106172011A CN106172011A CN201610711045.9A CN201610711045A CN106172011A CN 106172011 A CN106172011 A CN 106172011A CN 201610711045 A CN201610711045 A CN 201610711045A CN 106172011 A CN106172011 A CN 106172011A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- Developmental Biology & Embryology (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
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Abstract
The present invention relates to field of plant tissue culture technique, particularly a kind of dwarf banana high temperature light culture tissue culture and rapid propagation method.A kind of dwarf banana high temperature light culture tissue culture and rapid propagation method, comprises the following steps: outer implant preparation, successive transfer culture, root culture.The method subculture bud of the present invention is sprouted early, breaks up early, is broken up soon, and breeding coefficient is high, effective bud rate height；Seedling planting percent of taking root is high, plants seedling leaf and cauloid good toughness, root system difficult drop-off, heels in survival rate high.Utilizing high temperature light culture technology, subculture cycle shortens 10～15d, has minimizing energy consumption, improves space availability ratio, improves production efficiency, reduces production cost advantage.
Description
Technical field
The present invention relates to field of plant tissue culture technique, particularly a kind of dwarf banana high temperature light culture tissue-culturing rapid propagation side
Method.
Background technology
Dwarf banana (Musa ABB Pisang Awak), has another name called Annan any of several broadleaf plants, Saigon any of several broadleaf plants and milk any of several broadleaf plants etc., (is called for short perfume with fragrant tooth any of several broadleaf plants
Any of several broadleaf plants) (Musa AAA Cavendish) belong to the two big types of China staple food any of several broadleaf plants.Main flow kind " bronze 1 in the market
Number " owing to fruit skin is thin, sarcocarp color is yellowish or yellow, meat is sliding, and taste is sweet, has micro-perfume (or spice) and is liked by consumers in general.Closely
In the past few years dwarf banana not only rises year by year at south China consumption figure, and its northerly market also begins to open, and dwarf banana sales volume is gradually
Increase.Along with the expansion of dwarf banana industry, the dwarf banana seedling urgent need merchandized handling that high-quality is healthy, the seedling tissue-culturing rapid propagation skill of dwarf banana
Art just seems particular importance.In dwarf banana tissue-culturing rapid propagation in early days, the successive transfer culture stage exist breeding coefficient low, " Buds of The Pineapple " many,
The problems such as bubble bud ratio big, simple bud rate height, cycle length (reaching more than 40d)；The root culture stage exists that rooting rate is low, radical is few,
The problems such as root is long, root is easy to fall off, cause the training of dwarf banana group to produce and occur that variation risk is big, it is high to pollute, production efficiency is low and produces
High in cost of production drawback.Typically breeding coefficient is regulated by the phytohormone concentration in change culture medium in production.Improve plant
Hormone concentration can improve breeding coefficient, but can increase the risk of variation simultaneously；And the concentration reducing phytohormone can reduce change
Different rate, but breeding coefficient also decreases, all it cannot be guaranteed that rapid, high volume breeding health high-quality dwarf banana seedling.
At present, having the impact that tissue culture seedlings of bananas is grown by scholar's research different temperatures and different illumination, research shows temperature
Spend tissue culture seedlings of bananas is bred and taken root and all have pole significant impact, but to dwarf banana tissue cultured seedling illumination and the relevant report of temperature adjusting
Road is little.
The research team at inventor place compares, by regulation " bronze 1 " through practice for many years and test repeatedly
The temperature conditions of dwarf banana Seedling tissue-culturing rapid propagation and illumination condition, explore and obtain a kind of dwarf banana high temperature light culture tissue culture and rapid propagation method, should
Method maintains low aberration rate while obtaining higher breeding coefficient, it is ensured that the quality of seedling, improves production efficiency, enters
One step reduces production cost.
Summary of the invention
It is an object of the invention to provide a kind of dwarf banana high temperature light culture tissue culture and rapid propagation method, the method is obtaining higher breeding
Maintain low aberration rate while coefficient, it is ensured that the quality of seedling, improve production efficiency, reduce further and produce into
This.
Technical scheme provided by the present invention is:
A kind of dwarf banana high temperature light culture tissue culture and rapid propagation method, comprises the following steps:
(1) outer implant prepares and Primary culture: gathers the excellent elite stand of dwarf banana 50～100cm height and inhales bud, rinses through tap water
Totally, excise unnecessary leaves and stems, clean appearance 2～3 times with ethanol, superclean bench is peeled off sheath and is whittled into growing point
Centered by diameter 3cm, the cylinder scapus of high 5cm, then to separate the length of side from cylinder scapus center be 0.3～0.5cm
Blockage, is outer implant budlet block, is inoculated on Primary culture base cultivation 25～30d, obtains clump bud；
(2) successive transfer culture；The clump bud being activated cultivation is inoculated on subculture medium, cultivates under the conditions of high temperature light culture
25～30d；
(3) root culture: be first placed in root media cultivation 13～16d under the conditions of high temperature light culture；Put again
Under natural scattering optical condition, cultivate 15～25d, obtain tissue cultured seedling of taking root；
(4) seedling exercising: wait tissue cultured seedling length paramount 3～5cm of taking root, have 3～4 launch leaf time, it is moved in culturing room
Go out, be placed on and there is the outdoor shady and cool local of natural scattering optical condition, seedling exercising 7～10d, obtain Seedling of taking root；
(5) heel in: take out Seedling of taking root, wash away root culture medium, heel in and on husky bed, drench foot and determine root water, and hide in booth
The moon, periodically sprays water, and relative air humidity is 75～85%, and temperature is 27～35 DEG C.
As preferably, in step (2), step (3), high temperature light culture condition of culture is: temperature 32～35 DEG C, illumination be <
10Lux。
As preferably, step (1) and the Primary culture base of step (2) and subculture medium are MS culture medium+6-BA
2.0～3.0mg/L+IBA0.2mg/L.
As preferably, in step (3), root media is MS culture medium+NAA 1.0mg/L+IBA 0.2mg/L, pH
5.8。
As preferably, having substrate, described substrate in husky bed in step (5) is by pig manure: soil: peat soil is by volume
3:85:12 forms.
As preferably, the preparation method of described substrate is: measure pig manure, soil and peat soil, mixing, spreads according to seedling growth greenhouse
It is set as seedbed, covers the thick river sand of 2cm above, drench with 800 times of carbendazim and execute sterilization.
Compared with prior art, there is advantages that
(1) in the method for the present invention, cylinder scapus is not required to, again through sterilization, directly separate outer implant, improves work effect
Rate, avoids the injury of the external implant of the antiseptic solutions such as mercuric chloride simultaneously.
(2) the method subculture bud of the present invention is sprouted early, breaks up early, is broken up soon, and breeding coefficient is high, effective bud rate height.
(3) what the method for the present invention obtained take root, and Seedling planting percent is high, plants seedling leaf and cauloid good toughness, is not easily broken, and
Heel in survival rate high.
(4) the method subculture cycle of the present invention shortens 10～15d, has minimizing energy consumption, improves space availability ratio, improves raw
Produce efficiency, reduce production cost advantage.
Detailed description of the invention
Below in conjunction with concrete example, the present invention is further detailed explanation.
Embodiment 1:
A kind of dwarf banana high temperature light culture tissue culture and rapid propagation method, comprises the following steps:
(1) outer implant prepares and Primary culture: gathers dwarf banana excellent elite stand 50cm height and inhales bud, rinses well through tap water,
Excise unnecessary leaves and stems, clean appearance 2 times with ethanol, superclean bench is peeled off sheath and is whittled into centered by growing point
Diameter 3cm, the cylinder scapus of high 5cm, then separate, from cylinder scapus center, the blockage that the length of side is 0.3cm, outside being
The little sprout tuber of implant, is inoculated on Primary culture base cultivation 25d, obtains clump bud；Primary culture base is MS culture medium+6-BA
2.0mg/L+IBA0.2mg/L；
(2) successive transfer culture；The clump bud being activated cultivation is inoculated on subculture medium, cultivates under the conditions of high temperature light culture
25d；High temperature light culture condition of culture is: temperature 32 DEG C, and illumination is < 10Lux；Primary culture base and subculture medium are MS training
Support base+6-BA 2.0mg/L+IBA0.2mg/L；
(3) root culture: be first placed in root media cultivation 13d under the conditions of high temperature light culture；It is placed on again certainly
So cultivate 15d under the conditions of scattered light, obtain tissue cultured seedling of taking root；High temperature light culture condition of culture is: temperature 32 DEG C, illumination be <
10Lux；Root media is MS culture medium+NAA 1.0mg/L+IBA 0.2mg/L, pH 5.8；
(4) seedling exercising: wait the paramount 3cm of tissue cultured seedling length of taking root, have 3 launch leaf time, it is removed in culturing room, is placed on
There is the outdoor shady and cool local of natural scattering optical condition, seedling exercising 7d, obtain Seedling of taking root；
(5) heel in: take out Seedling of taking root, wash away root culture medium, heel in and on husky bed, drench foot and determine root water, and hide in booth
The moon, periodically sprays water, and relative air humidity is 75%, and temperature is 27 DEG C；Having substrate, described substrate in husky bed is by pig manure: soil:
Peat soil forms for 3:85:12 by volume；The preparation method of described substrate is: measure pig manure, soil and peat soil, mixing,
It is laid to seedbed according to seedling growth greenhouse, covers the thick river sand of 2cm above, drench with 800 times of carbendazim and execute sterilization.
Embodiment 2:
A kind of dwarf banana high temperature light culture tissue culture and rapid propagation method, comprises the following steps:
(1) outer implant prepares and Primary culture: gathers dwarf banana excellent elite stand 100cm height and inhales bud, rinses well through tap water,
Excise unnecessary leaves and stems, clean appearance 3 times with ethanol, superclean bench is peeled off sheath and is whittled into centered by growing point
Diameter 3cm, the cylinder scapus of high 5cm, then separate, from cylinder scapus center, the blockage that the length of side is 0.5cm, outside being
The little sprout tuber of implant, is inoculated on Primary culture base cultivation 30d, obtains clump bud；Primary culture base is MS culture medium+6-BA
3.0mg/L+IBA0.2mg/L；
(2) successive transfer culture；The clump bud being activated cultivation is inoculated on subculture medium, cultivates under the conditions of high temperature light culture
30d；High temperature light culture condition of culture is: temperature 35 DEG C, and illumination is < 10Lux；Primary culture base and subculture medium are MS training
Support base+6-BA3.0mg/L+IBA0.2mg/L；
(3) root culture: be first placed in root media cultivation 16d under the conditions of high temperature light culture；It is placed on again certainly
So cultivate 25d under the conditions of scattered light, obtain tissue cultured seedling of taking root；High temperature light culture condition of culture is: temperature 35 DEG C, illumination be <
10Lux；Root media is MS culture medium+NAA 1.0mg/L+IBA 0.2mg/L, pH 5.8；
(4) seedling exercising: wait the paramount 5cm of tissue cultured seedling length of taking root, have 4 launch leaf time, it is removed in culturing room, is placed on
There is the outdoor shady and cool local of natural scattering optical condition, seedling exercising 10d, obtain Seedling of taking root；
(5) heel in: take out Seedling of taking root, wash away root culture medium, heel in and on husky bed, drench foot and determine root water, and hide in booth
The moon, periodically sprays water, and relative air humidity is 85%, and temperature is 35 DEG C；Having substrate, described substrate in husky bed is by pig manure: soil:
Peat soil forms for 3:85:12 by volume；The preparation method of described substrate is: measure pig manure, soil and peat soil, mixing,
It is laid to seedbed according to seedling growth greenhouse, covers the thick river sand of 2cm above, drench with 800 times of carbendazim and execute sterilization.
Embodiment 3:
A kind of dwarf banana high temperature light culture tissue culture and rapid propagation method, comprises the following steps:
(1) outer implant prepares and Primary culture: gathers dwarf banana excellent elite stand 80cm height and inhales bud, rinses well through tap water,
Excise unnecessary leaves and stems, clean appearance 3 times with ethanol, superclean bench is peeled off sheath and is whittled into centered by growing point
Diameter 3cm, the cylinder scapus of high 5cm, then separate, from cylinder scapus center, the blockage that the length of side is 0.4cm, outside being
The little sprout tuber of implant, is inoculated on Primary culture base cultivation 28d, obtains clump bud；Primary culture base is MS culture medium+6-BA
2.5mg/L+IBA0.2mg/L；
(2) successive transfer culture；The clump bud being activated cultivation is inoculated on subculture medium, cultivates under the conditions of high temperature light culture
28d；High temperature light culture condition of culture is: temperature 33 DEG C, and illumination is < 10Lux；Primary culture base and subculture medium are MS training
Support base+6-BA 2.2mg/L+IBA0.2mg/L；
(3) root culture: be first placed in root media cultivation 15d under the conditions of high temperature light culture；It is placed on again certainly
So cultivate 20d under the conditions of scattered light, obtain tissue cultured seedling of taking root；High temperature light culture condition of culture is: temperature 33 DEG C, illumination be <
10Lux；Root media is MS culture medium+NAA 1.0mg/L+IBA 0.2mg/L, pH 5.8；
(4) seedling exercising: wait the paramount 4cm of tissue cultured seedling length of taking root, have 3 launch leaf time, it is removed in culturing room, is placed on
There is the outdoor shady and cool local of natural scattering optical condition, seedling exercising 8d, obtain Seedling of taking root；
(5) heel in: take out Seedling of taking root, wash away root culture medium, heel in and on husky bed, drench foot and determine root water, and hide in booth
The moon, periodically sprays water, and relative air humidity is 80%, and temperature is 30 DEG C；Having substrate, described substrate in husky bed is by pig manure: soil:
Peat soil forms for 3:85:12 by volume；The preparation method of described substrate is: measure pig manure, soil and peat soil, mixing,
It is laid to seedbed according to seedling growth greenhouse, covers the thick river sand of 2cm above, drench with 800 times of carbendazim and execute sterilization.
Embodiment 4: the implementation result of the method for the present invention
Condition of culture: 3 thermogrades and 2 lighting gradients, respectively high temperature (32-35 DEG C), middle temperature (28-30 are set
DEG C), room temperature (24-26 DEG C)；Light (2000Lux, illumination 12h/d), dark (< 10Lux hides upper all curtain daytime, does not turn on light i.e.
Can).6 different illumination and treatment of different temperature combination are set altogether, are respectively as follows: high temperature light culture (method of the present invention), high temperature
Light cultivates (comparison 1), middle temperature light culture (comparison 2), middle temperature light cultivates (comparison 3), room temperature light culture (comparison 4) and normal-temperature light training
Support (comparison 5).
It should be noted that in the process of comparison 1-5, the condition of culture in step (2), step (3) is different, other step
Operation all identical with embodiment 1-3.
The method of 4.1 present invention is on subculture cycle and the impact of breeding coefficient
The impact on successive transfer culture breeding coefficient of the method for table 1 present invention
Note: same column difference letter representation exists significant difference (P < 0.05) between processing, lower same.
As shown in Table 1, high temperature light culture processes breeding coefficient and reaches about 2.0 (1.98) at inoculation 12d, and to impinging upon inoculation
Just reaching about 2.0 (respectively 2.01,2.01,1.91,2.02,1.97) during 25d, high temperature light culture processes faster than control treatment
13d, illustrate high temperature light culture can promote the bud to sprout, break up early, differentiation fast.Subculture Bud polarization is fast, and blastogenesis is long at short notice
Time the most, bud is just relatively strong, can shorten subculture cycle, and the production subculture cycle of dwarf banana group training in early days is more than 40d, and sharp
By high temperature light culture technology, subculture cycle shortens to 25～30d, saves the successive transfer culture time of 10～15d.Successive transfer culture
After 25d, the breeding coefficient of embodiments of the invention 1-3 reaches as high as 2.62, is all higher than other 5 control treatment, this is described
The method for tissue culture of bright dwarf banana can improve breeding coefficient.
The impact on subculture bud quality of the method for 4.2 present invention
The method of table 2 present invention becomes the impact of bud rate and growing state to subculture bud
As shown in Table 2, embodiments of the invention 1-3 effective bud rate is maximum, is 96.74%, the dwarf banana of the present invention is described
Method for tissue culture can improve effective bud rate.
Blade and the root grown that subculture bud launches increase the risk of pollution when transferring, increase workload, reduce work effect
Rate.It is 0 that embodiments of the invention 1-3 bud launches the number of blade, more has beneficially subculture, reduces pollution risk during switching, improves
Work efficiency.
Foaming bud is to weigh the important indicator that successive transfer culture bud is abnormal and makes a variation, and foaming bud is the most, and subculture bud is described
The most abnormal, the risk of variation is the biggest, and bud foaming is also unfavorable for further transferring.Embodiments of the invention 1-3 foaming bud rate is
Little, subculture bud quality is good.
The method of 4.3 present invention is on Seedling and the impact heeled in of taking root
The impact on take root Seedling planting percent and growing state of the method for table 3 present invention
As shown in table 3, the planting percent of taking root of the method root culture of the present invention is the highest, all reaches the planting percent of 100%, aobvious
Writing higher than control treatment, Seedling height of seedling of taking root, the radical of average every young plant and the expansion number of blade all can reach production requirement.
The impact on husky seedlings cultivating survival rate of the method for table 4 present invention
Seedling is heeled on preprepared sand bed, after heeling in 3 weeks, survival rate is investigated.As shown in table 4,
The method of the present invention obtains processing shoot survival percent and is 100%, higher than other matched group.
The method of 4.4 present invention is on energy consumption and the impact of space utilization situation
With the culturing rack of a width of 1.5m × 0.5m of monolayer length in production, 4 layers of each shelf.The method of the present invention is trained at subculture
In the stage of supporting, culture bottle can place with stacking, and every frame can put 1008 bottles, and during light cultivation, culture bottle can not stack, and every frame can only
Putting 432 bottles, therefore, the method for the present invention relatively light training method space availability ratio improves 133.33%.Every layer of frame when light is cultivated
Son installs the fluorescent tube of 2 40W, and culturing rack each successive transfer culture cycle (unified by 25d calculating) converts into electricity consumption 96.00 degree,
The method of the present invention then can save this part energy consumption, also enhances production security simultaneously.Therefore, subculture light culture pair
Reduce energy consumption, improve space availability ratio, raising group training production efficiency, reduction production cost and remarkable result of having increased the benefit.
In sum, the method subculture bud of the present invention is sprouted early, breaks up early, is broken up soon, and breeding coefficient is high, effective bud rate
High；Seedling planting percent of taking root is high, plants seedling leaf and cauloid good toughness, root system difficult drop-off, heels in survival rate high.High temperature is utilized secretly to train
The technology of supporting, subculture cycle shortens 10～15d, has minimizing energy consumption, improves space availability ratio, improves production efficiency, reduces raw
Produce cost advantages.
The aforementioned description to the specific illustrative embodiment of the present invention illustrates that and the purpose of illustration.These describe
It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to above-mentioned teaching, can much change
And change.The purpose selected exemplary embodiment and describe is to explain that the certain principles of the present invention and reality thereof should
With so that those skilled in the art be capable of and utilize the present invention various different exemplary and
Various different selections and change.The scope of the present invention is intended to be limited by claims and equivalents thereof.
Claims (6)
1. a dwarf banana high temperature light culture tissue culture and rapid propagation method, it is characterised in that comprise the following steps:
(1) outer implant prepares and Primary culture: gathers the excellent elite stand of dwarf banana 50～100cm height and inhales bud, rinses well through tap water,
Excise unnecessary leaves and stems, clean appearance 2～3 times with ethanol, in superclean bench being peeled off sheath and being whittled into and with growing point be
The diameter 3cm of the heart, the cylinder scapus of high 5cm, the less side that the length of side is 0.3～0.5cm is separated from cylinder scapus center
Block, is outer implant budlet block, is inoculated on Primary culture base cultivation 25～30d, obtains clump bud；
(2) successive transfer culture；The clump bud being activated cultivation is inoculated on subculture medium, under the conditions of high temperature light culture cultivate 25～
30d；
(3) root culture: be first placed in root media cultivation 13～16d under the conditions of high temperature light culture；It is placed on again certainly
So cultivate 15～25d under the conditions of scattered light, obtain tissue cultured seedling of taking root；
(4) seedling exercising: wait tissue cultured seedling length paramount 3～5cm of taking root, have 3～4 launch leaf time, it is removed in culturing room, puts
There is the outdoor shady and cool local of natural scattering optical condition, seedling exercising 7～10d, obtaining Seedling of taking root；
(5) heel in: take out Seedling of taking root, wash away root culture medium, heel in and on husky bed, drench foot and determine root water, and shelter from heat or light in booth, fixed
Phase sprays water, and relative air humidity is 75～85%, and temperature is 27～35 DEG C.
Dwarf banana high temperature light culture tissue culture and rapid propagation method the most according to claim 1, it is characterised in that step (2), step
(3) in, high temperature light culture condition of culture is: temperature 32～35 DEG C, and illumination is < 10Lux.
Dwarf banana high temperature light culture tissue culture and rapid propagation method the most according to claim 1, it is characterised in that step (1) and step
(2) Primary culture base and subculture medium are MS culture medium+6-BA2.0～3.0mg/L+IBA0.2mg/L.
Dwarf banana high temperature light culture tissue culture and rapid propagation method the most according to claim 1, it is characterised in that step is taken root in (3)
Culture medium is MS culture medium+NAA1.0mg/L+IBA0.2mg/L, pH 5.8.
Dwarf banana high temperature light culture tissue culture and rapid propagation method the most according to claim 1, it is characterised in that husky bed in step (5)
In have substrate, described substrate is by pig manure: soil: peat soil forms for 3:85:12 by volume.
Dwarf banana high temperature light culture tissue culture and rapid propagation method the most according to claim 1, it is characterised in that described in step (5)
The preparation method of substrate is: measure pig manure, soil and peat soil, mixing, is laid to seedbed according to seedling growth greenhouse, covers 2cm above thick
River sand, drench with 800 times of carbendazim and execute sterilization.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106688887A (en) * | 2016-12-08 | 2017-05-24 | 广东省农业科学院果树研究所 | Fenza No.1 dwarf banana tissue culture rapid propagation method |
| CN106922537A (en) * | 2017-04-21 | 2017-07-07 | 中国热带农业科学院海口实验站 | A kind of cultural method and its culture medium of kylin fruit seedling |
| CN113142055A (en) * | 2021-04-29 | 2021-07-23 | 广西壮族自治区农业科学院 | In-vitro proliferation preservation method for banana germplasm resources |
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| CN102726302A (en) * | 2012-07-18 | 2012-10-17 | 广西植物组培苗有限公司 | Dark culture method for banana tissue culture |
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| CN102726302A (en) * | 2012-07-18 | 2012-10-17 | 广西植物组培苗有限公司 | Dark culture method for banana tissue culture |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106688887A (en) * | 2016-12-08 | 2017-05-24 | 广东省农业科学院果树研究所 | Fenza No.1 dwarf banana tissue culture rapid propagation method |
| CN106688887B (en) * | 2016-12-08 | 2019-08-20 | 广东省农业科学院果树研究所 | The tissue culture and rapid propagation method of miscellaneous No. 1 dwarf banana of powder |
| CN106922537A (en) * | 2017-04-21 | 2017-07-07 | 中国热带农业科学院海口实验站 | A kind of cultural method and its culture medium of kylin fruit seedling |
| CN113142055A (en) * | 2021-04-29 | 2021-07-23 | 广西壮族自治区农业科学院 | In-vitro proliferation preservation method for banana germplasm resources |
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| Publication number | Publication date |
|---|---|
| CN106172011B (en) | 2018-06-22 |
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